• Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
  • The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
  • Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
  • This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
  • Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
  • Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. (rndsystems.com)
  • Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
  • ALA induced erythroid differentiation of human myelogenous leukemia K562 and murine erythroleukemia MEL cells, leads to hemoglobinization and cellular typical maturation. (longdom.org)
  • Hemoglobin synthesis and assembly is largely dependent on coordinated gene expression control, it is well established that the histone deacetylase inhibitor butyric acid activates transcription of heme synthesis an globin mRNA expression, leading to changes in cell morphology and induction of erythroid differentiation of erythroleukemic cell lines. (longdom.org)
  • K562 cells were the first human immortalised myelogenous leukemia cell line to be established. (wikipedia.org)
  • K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. (wikipedia.org)
  • K562 cells can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes and are easily killed by natural killer cells as they lack the MHC complex required to inhibit NK activity. (wikipedia.org)
  • K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
  • The change in phenotype induces a decrease in the growth rate and leads the K562 cells to the terminal path of becoming mature erythroids, monocytes, and mature macrophages. (wikipedia.org)
  • The problem with K562 cells, and many other cancer cell types, is an overabundance of Aurora kinases. (wikipedia.org)
  • Apoptosis is an important mechanism in regulating K562 cells and can be induced by the changes in the metabolic state of the cells. (wikipedia.org)
  • There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
  • The offset of these cellular components from their balance point causes morphological changes, which result in the K562 cells being arrested in the G2/M phase of the cell cycle. (wikipedia.org)
  • The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)
  • Another important group of regulators of the K562 line are Sirtuins, referred to as SIRTS. (wikipedia.org)
  • In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
  • Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
  • Recently we demonstrated that downregulation or over expression of ALA dehydratase (ALAD) and porphobilinogen deaminase (PBGD), the second and the third enzymes of the pathway, by specific shRNAs caused a marked alteration in PpIX synthesis in K562 erythroleukemic cells. (longdom.org)
  • Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. (rndsystems.com)
  • Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody. (rndsystems.com)
  • Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC ) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). (rndsystems.com)
  • Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). (rndsystems.com)
  • Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. (medchemexpress.com)
  • Toxicity evaluation of the pesticide azadirachtin on the insect cells, SF-9 and the murine erythroleukemic cells, GM-86. (lafayette.edu)
  • Fli-1, a known person in the ETS transcription aspect family members, is also the mark of insertional activation by Friend murine leukemia trojan (F-MuLV) and it is preferentially portrayed in vascular endothelial cells and hematopoietic tissue [6]. (columbiagypsy.net)
  • Heme synthesis enhances globin formation, not only because it is the precursor of Hb but also by modulating the machinery of gene expression and globin synthesis and coordinating erythroid cell maturation. (longdom.org)
  • Many ORs are expressed by tumor cells and tissues, suggesting that they may be associated with cancer progression or may be cancer biomarkers. (bmbreports.org)
  • 323. The Neutralization Dose (ND 50 ) is typically 0.5-1.5 µg/mL in the presence of 0.3 units/mL Recombinant Human Erythropoietin/EPO (Tissue Culture Grade). (rndsystems.com)
  • Recombinant protein fragment within Human ATRX. (arigobio.com)
  • 29)), and Eq. Vascular endothelial cell growth factor activates CRE-binding protein by signaling through the KDR receptor tyrosine kinase. (forex-consultant.com)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
  • The genotoxic effects of taxol on mouse erythroleukemia cells. (lafayette.edu)
  • However, the vast majority of biological knowledge has emerged as a consequence of studying cell populations and not individual cells. (justia.com)
  • The IFNA2 (interferon alpha 2) gene is mapped to human chromosome 9p21.3. (sigmaaldrich.com)
  • The gene for human PF4 is located on human chromosome 4 . (wikidoc.org)
  • Isolation of the human cDNA and genomic DNA revealed that the gene was located on chromosome 8p21, a region implicated in numerous leukemias and solid tumors. (edu.au)
  • Inhibiting these is an important regulation mechanism of cancer, because it prevents cells from progressing into mitosis. (wikipedia.org)
  • This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. (arigobio.com)
  • This arrest leads to "shrinkage, blebbing, nuclear fragmentation, chromatin condensing" and other morphological changes that cause the cell to program death at this point. (wikipedia.org)
  • Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. (arigobio.com)
  • Interferon α 2A (IFN-α 2A) belongs to the type I interferon protein family and is produced by many cell types in response to viral infection. (sigmaaldrich.com)
  • Cytotoxic evaluation of Raloxifene, a protein antibreast cancer drug, on human and mouse cancer cell lines. (lafayette.edu)
  • It is the second-most abundant protein after actin, comprising 1-3% of the total protein content in normal growing cells [ 7 , 8 ]. (biomedcentral.com)
  • An accurate white cell count and differential should be performed on CSF and the protein, glucose concentrations should be measured. (forex-consultant.com)
  • Toxic and antitumorigenic potential of tamoxifen, raloxifene, and the combination on 4t1 mouse breast cancer cells in vitro and in balb/c-j female mice in vivo. (lafayette.edu)
  • Where drug pharmacokinetics becomes an important factor, this statement has potential implications for sensitization of cyst cells in vivo. (hdacassay.com)
  • Identification of CP466722 Urogenital pelvic malignancy supplies a new chemical structure that inhibits ATM function in cells and may now be modified to generate more potent and specific agencies that could possibly be able to enhancing cyst cell killing in vivo. (hdacassay.com)
  • Hemopoietic cells, apparently committed to one lineage, can be reprogrammed to display the phenotype of another lineage. (edu.au)
  • Anti-FYa and anti-FYb antibodies define four red blood cell (RBC) phenotypes: FYa + FYb-, FYa-FYb+, FYa + FYb+, and FYa-FYb- [ 2 ]. (biomedcentral.com)
  • IFN-α 2A enhances the expression of MHC proteins by increasing the presentation of antigen peptides and activates cytotoxic T cells. (sigmaaldrich.com)
  • This latter observation highlights components of these DDR paths as possible therapeutic targets for the development of small molecule inhibitors that could enhance the sensitivity of tumefaction cells to the cytotoxic ramifications of radio /chemo therapeutic agents. (hdacassay.com)
  • For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
  • Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
  • Microfluidic devices employing active valving to position and isolate cells have allowed for the isolation and genome amplification of individual microbial cells (32). (justia.com)
  • Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)
  • We report here on the isolation of hemopoietic lineage switch 5 (Hls5), a gene expressed by the monocytoid variant cells, but not the parental J2E cells. (edu.au)
  • By transfection of cell lines mc14 and mc20, the reactivating functions were shown to reside in the pAd2E1A-13 S cDNA clone of Ad2 DNA. (uni-bielefeld.de)
  • The modification of the three 5' CCGG 3' sequences at nucleotides +24, +6 and -215, relative to the cap site in this promoter, sufficed to silence the gene in transient expression either in Xenopus laevis oocytes or in mammalian cells, and after the fixation of the E2A promoter-chloramphenicol-acetyltransferase (CAT) gene construct in the genome of hamster cells. (uni-bielefeld.de)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • The usage of vector modified hematopoietic stem cell therapy by which myelocytotoxic and IS drugs are given to the host to create space in the bone marrow for the homing and development of gene adjusted cells will not be examined. (hdacassay.com)
  • T and B cell tolerance can be recognized or damaged either centrally, at the website of primary lymphocyte growth in the thymus or bone marrow, or peripherally in the lymphoid tissue where antigen recognition and processing occur. (hdacassay.com)
  • LC-06-JCK-bearing mice showed high red blood cell counts and erythropoietin levels as compared to NTB mice, whereas MR16-1 treatment did not affect their levels. (biomedcentral.com)
  • To clarify the hypothesis that overproduction of IL-6 elevates hepcidin levels and contributes to the development of cancer-related anemia, we evaluated anti-IL-6 receptor antibody treatment of cancer-related anemia in an IL-6-producing human lung cancer xenograft model. (biomedcentral.com)
  • Nude mice were subcutaneously inoculated with cells of the IL-6-producing human lung cancer cell line LC-06-JCK and assessed as a model of cancer-related anemia. (biomedcentral.com)
  • Novel human renal proximal tubular cell line for the production of complex proteins. (rndsystems.com)
  • Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. (medchemexpress.com)
  • These changes can also drive the leukemic cells to a state of stress, which allows for increased sensitivity of the cells to drugs that initiate apoptosis. (wikipedia.org)
  • When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
  • The aim of this study was to test for the molecular and functional expression of the non-gastric H + /K + ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM) human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H + /K + ATPase inhibitor SCH28080 in apoptosis. (karger.com)
  • Of these, single cell RT-qPCR provides combined advantages of sensitivity, specificity, and dynamic range, but is limited by low throughput, high reagent cost, and difficulties in accurately measuring low abundance transcripts (6). (justia.com)
  • Microfluidic systems provide numerous advantages for single cell analysis: economies of scale, parallelization and automation, and increased sensitivity and precision that comes from small volume reactions. (justia.com)
  • Studies of cells that are functionally mGluR defective in different components of the DDR pathways}, reduced ability to repair damaged DNA and a heightened sensitivity to IR and other DNA damaging agents. (hdacassay.com)
  • CP466722 is just a fast reversible inhibitor of ATM function and transient coverage used in clonogenic survival assays suggests that temporary inhibition of ATM function is enough to sensitize cells to IR. (hdacassay.com)
  • The immune systems a reaction to antigen depends on the relative frequencies of responding T and Caspase inhibition B cells and on the thresholds of binding affinity that their receptors display, the quantities of antigen present, and the period when the antigen stays in secondary lymphoid tissue, where primary immune responses are initiated. (hdacassay.com)
  • Earlier KLHL1 antibody studies have shown that knocking-down Fli-1 prospects to designated growth inhibition and death in erythroleukemic cells, indicating a possible use of Fli-1 like a restorative target to induce tumor suppression [18C20]. (columbiagypsy.net)
  • Recent achievements highlight the accelerated development of epigenetics, such as the definition of a human DNA methylome at single-nucleotide resolution, the various discoveries of histone variants and modifications, the study of the CpG island in the genome, and the progress of genome-wide nucleosome positioning maps ( Baldi, 2019 ). (frontiersin.org)
  • Single cells represent a fundamental biological unit. (justia.com)
  • IL-6 exerts various biological activities on responding cell populations through its binding to transmembrane IL-6 receptor as well as to soluble IL-6 receptor. (biomedcentral.com)
  • These functions are necessary in cells in order to divide and regenerate tissues, and play a maintenance role in homeostatic functions. (wikipedia.org)
  • In addition, ORs were recently shown to be expressed in many nonolfactory tissues and cells, indicating that these receptors have physiological and pathophysiological roles beyond olfaction. (bmbreports.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Moreover, the device does not allow isolated cells to be washed from the supernatant prior to treatment or analysis. (justia.com)
  • Glycosylation contributes to stability in cell growth media and other applications. (sigmaaldrich.com)
  • Enforced expression of Hls5 in HeLa cells inhibited cell growth, clonogenicity, and tumorigenicity. (edu.au)
  • The ATM signal transduction was also inhibited by the compound pathway in cells, disturbed cell cycle checkpoint function and sensitive tumefaction cells to IR. (hdacassay.com)
  • Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
  • These play a role in cellular stress, metabolism, and autophagy, by interacting with deacetylases activity in the cell. (wikipedia.org)
  • The CEG/DEG database identified 662 essential genes that drastically reduced to 68 genes after non-homology analyses towards human and gut microbiome. (bvsalud.org)
  • Provided herein are methods and compositions useful for the replenishment of blood cells in a mammal after exposure to therapeutic radiation or drugs. (patentsencyclopedia.com)
  • IFNA2 is a type 1 interferon which acts as the first line of defense, and offers protection against pathogenic invasion. (sigmaaldrich.com)
  • Itens: Toxicity evaluation of selenium compounds on human prostate cancer and mouse erythroleukemic cells. (lafayette.edu)
  • Toxicity evaluation of selenium compounds on human prostate cancer and mouse erythroleukemic cells. (lafayette.edu)
  • Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
  • In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
  • Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
  • Prejudice describes the problem whereby T cells neglect to react to a certain antigen. (hdacassay.com)
  • This is due to low degrees of antigen that are insufficient to activate T cells, antigens that are physically separated from T cells. (hdacassay.com)
  • Dysregulated continuous production of IL-6 by a distinct cell population plays a pathological role in various inflammatory autoimmune diseases. (biomedcentral.com)
  • The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. (justia.com)
  • The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. (justia.com)