Human epidermal keratinocytes. Medical search. Frequent questions

Keratinocyte proliferation, differentiation, and programmed cell death are complex and carefully choreographed processes. (3hbiomedical.com)
Collagen-PCL Sheath-Core Bicomponent Electrospun Scaffolds Increase Osteogenic Differentiation and Calcium Accretion of Human Adipose-Derived Stem Cells. (ncsu.edu)
Human adipose-derived stem cells (hASCs) are an abundant cell source capable of osteogenic differentiation, and have been investigated as an autologous stem cell source for bone tissue engineering applications. (ncsu.edu)
We exploit this method to spectroscopically observe chemical changes that occur during the adipogenic differentiation of human-adipose-derived stem cells over a period of 22 days. (ncsu.edu)
Keratinocyte transglutaminase enzymes serve to specifically catalyze the development of the cornified cell envelope, a defining characteristic of epidermal keratinocytes that have undergone the termination of differentiation. (wikipedia.org)
Psoriasin, which is highly expressed in psoriasis, is encoded by a gene located within the epidermal differentiation complex. (medicaljournals.se)
The aim of this study was to investigate the effect of endogenous psoriasin on disturbed keratinocyte differentiation in psoriasis. (medicaljournals.se)
Induction of keratinocyte differentiation caused concurrent expression of psoriasin and the differentiation marker involucrin. (medicaljournals.se)
To establish the skin barrier and the epidermal structure, keratinocytes undergo a tightly regulated process of differentiation. (medicaljournals.se)
In psoriasis, the differentiation process of the epidermal cells is disturbed. (medicaljournals.se)
The keratinocytes in active psoriatic lesions display an altered sequence and altered expression levels of the differentiation markers (2). (medicaljournals.se)
However, the mediators that are responsible for the dysregulated differentiation of psoriatic keratinocytes have not been identified. (medicaljournals.se)
In a spectrum of conditions of dysregulated keratinocyte differentiation, we have previously demonstrated strong psoriasin expression in differentiated squamous cell carcinoma and a lack of expression in undifferentiated basalioma (9). (medicaljournals.se)
In addition, the gene that encodes psoriasin is positioned within the epidermal differentiation complex (EDC) (10-12), a gene cluster that contains several genes of importance in the terminal differentiation of the human epidermis (10, 11). (medicaljournals.se)
The high expression level in psoriatic epidermis in conjunction with the disturbed differentiation process suggests that psoriasin is involved in keratinocyte differentiation. (medicaljournals.se)
Previous studies of the role of psoriasin in keratinocyte differentiation have focused on the effects of extracellular psoriasin, but as the psoriasin in psoriasis is keratinocyte-derived, it is imperative to consider the effects of psoriasin as an endogenously produced intracellular mediator. (medicaljournals.se)
In psoriasis, the over-proliferation of basal layer keratinocytes (KCs) is coupled with differentiation and cornification defects in the upper layers, leading to plaque formation ( 7 ). (frontiersin.org)
This cell line has been known to retain a capacity fornormal differentiation up to multiple passages similar to normal human epidermal keratinocytes (NHEK), and hence offers a suitable model for keratinisation studies. (reprocell.com)
The epidermal growth factor receptor (EGFR) regulates the proliferation and differentiation of keratinocytes. (europa.eu)
To elucidate RIPK4 function in epidermal differentiation and development, we generated epidermis-specific RIPK4 KO mice (RIPK4 EKO). (ugent.be)
As it maintains an epidermal differentiation capacity, it has been used as a model for keratinization studies. (bvsalud.org)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)

They are not approved for human or animal use, or for application in in vitro diagnostic procedures. (3hbiomedical.com)
Water-soluble proteins of royal jelly were fractionated and investigated for the proliferative and migratory effects on human epidermal keratinocytes (HaCaT) in an in vitro wound healing model. (biomedcentral.com)
Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet', Iranian Journal of Dermatology , 9(1), pp. 2-16. (iranjd.ir)
This test uses the EpiDerm™ reconstructed human epidermis model (MatTek) which consists of normal human-derived epidermal keratinocytes (NHEK) and therefore represents in vitro the target organ of the species of interest and closely resembles the biochemical and physiological properties of the upper parts of the human skin, i.e. the epidermis. (europa.eu)
In vitro generation of human pluripotent stem cell derived lung organoids. (nature.com)
Keratinocytes expanded under these conditions have an increased long-term regenerative potential in in vitro epidermal reconstruction models and in vivo grafts2. (cea.fr)
Through in vitro studies, it was found that epi-oxyzoanthamine inhibited the expression of cytokines in TNF-α/IFN-Î³-stimulated human keratinocyte (HaCaT) cells, and it reduced the phosphorylation of MAPK and the NF-κB signaling pathway. (bvsalud.org)

The EpiDerm™ EPI-200 skin model consists of normal human epidermal keratinocytes (NHEK) from one single donor, derived from neonatal-foreskin tissue. (europa.eu)

A CEA-Jacob team has just published a paper in which it demonstrates the central role of the transcription factor KLF4 in regulating the proliferation of epidermal stem cells and their ability to regenerate this tissue. (cea.fr)
The study has shown that manipulation of KLF4 expression is also suitable for these cells, as reducing its expression in keratinocytes derived from embryonic stem cells (ESC) improves their proliferation capacity and their ability to reconstruct skin. (cea.fr)
Sustained hypoxia or lack of nutrients leads to fibroblast dysfunction, decreased keratinocyte migration and proliferation, and tissue loss [ 6 ]. (hindawi.com)
Psoriasis lesions are characterized by hyper-proliferation of epidermal keratinocytes associated with inflammatory cellular infiltrate in both dermis and epidermis. (springer.com)
Due to its role in proliferation and maturation of keratinocytes, vitamin D has become an important local therapeutic option in the treatment of psoriasis. (springer.com)
c-Src potentiates proliferation, survival, and invasiveness in response to epidermal growth factor (EGF) in human mammary carcinoma cells. (aspetjournals.org)

ZIKV infection (African lineage MR766) in human dermal fibroblasts, human epidermal keratinocytes, and HaCaT keratinocytes resulted in distinct expression changes in RIG-I-like receptors, such as RIG-I and MDA5. (korea.ac.kr)
HaCaT is an immortalised human adult keratinocyte cell line. (reprocell.com)

Histologically, the dermatosis is characterized by hyperproliferation of keratinocytes, impaired epidermal barrier function at the sites of skin lesions, and skin infiltration by activated inflammatory cells [ 5 ]. (springer.com)

The predominant cell type in the epidermis is keratinocytes and they are located in the stratified squamous epithelia. (3hbiomedical.com)
Progenitors of keratinocytes reside and divide in the basal layer of the epidermis. (3hbiomedical.com)
In this process, the keratinocytes progress from the basal layer of the epidermis through distinct stages to form the spinous, granular and cornified layers. (medicaljournals.se)
A keratinocyte is the main type of cell that makes up the human epidermis, the outermost layer of skin, he said. (cbs58.com)
Psoriasis is hyperproliferation of epidermal keratinocytes combined with inflammation of the epidermis and dermis. (merckmanuals.com)
Furthermore, our data indicate that epidermis-specific deletion of RIPK4 results in delayed keratinization and stratum corneum maturation and altered lipid organization and is thus indispensable during embryonic development for the formation of a functional inside-out epidermal barrier. (ugent.be)
Human and in the epidermis, form the major protective barrier that pre- animal studies of atopic dermatitis have identified skin epithelial vents loss of water and protects against foreign substances. (cdc.gov)
The barrier dysfunction, which may facilitate allergen entry, as a key epidermis also contains keratinocytes and Langerhans cells, a factor in the development of atopic asthma and sensitization. (cdc.gov)
Psoriasis is a complex, chronic, multifactorial, inflammatory disease that involves hyperproliferation of the keratinocytes in the epidermis, with an increase in the epidermal cell turnover rate (see the image below). (medscape.com)

In Situ Monitoring of Adipogenesis with Human-Adipose-Derived Stem Cells Using Surface-Enhanced Raman Spectroscopy. (ncsu.edu)
Methods capable of nondestructively collecting high-quality, real-time chemical information from living human stem cells are of increasing importance given the escalating relevance of stem cells in therapeutic and regenerative medicines. (ncsu.edu)
This report uses 40-nm colloidal gold nanoparticles to create volumes of surface-enhanced Raman scattering (SERS) within living human-adipose-derived adult stem cells enabling molecular information to be monitored. (ncsu.edu)
Asian lineage ZIKV (PRVABC59) infection also showed a distinct pattern of antiviral immunity in human skin cells, compared with other ZIKV strains. (korea.ac.kr)
Additionally, ZIKV infections in human neural progenitor cells induced the robust activation of RIG-I-like receptor-mediated signaling, followed by highly enhanced IFN-stimulated gene expression. (korea.ac.kr)
Regulation of cyclooxygenase-2 by interferon γ and transforming growth factor α in normal human epidermal keratinocytes and squamous carcinoma cells. (houstonmethodist.org)
The study population comprised 21 symptomatic and ment might be attributable to Zika virus replication in per- consenting Zika virus patients infected during MarchSep- missive cells of the skin (e.g., human dermal fibroblasts tember 2016. (cdc.gov)
Identification of multipotent luminal progenitor cells in human prostate organoid cultures. (nature.com)
Human skin completely renews itself every month thanks to the presence of stem cells in the deepest layer, which generate all the upper layers of this tissue. (cea.fr)
Massive ex vivo expansion of epidermal cells (called keratinocytes) is needed for the production of grafts. (cea.fr)
It is performed using a skin sample from the patient that contains adult keratinocytes and a minority population of keratinocyte stem cells. (cea.fr)
In the future, keratinocytes produced from pluripotent stem cells could be an alternative to adult stem cells in certain reconstructed tissue bio-engineering applications. (cea.fr)
One of the difficulties encountered in this area is that fact that the keratinocytes obtained do not have all the functions of adult stem cells. (cea.fr)
The video shows epidermal cells at the edge of an epithelial sheet in culture. (cellimagelibrary.org)
We previously showed that genotoxic stress induced an active extracellular release of nucleophosmin (NPM) in human cardiac mesenchymal progenitor cells, and that serum deprivation provokes NPM secretion from human endothelial cells, eliciting inflammation via nuclear factor kappa B (NF-kB) transcriptional activation. (frontiersin.org)
Here we show that bivalent domains and chromosome architecture for bivalent genes are dynamically regulated during the cell cycle in human pluripotent cells. (researchgate.net)
Pachyonychia congenita (PC) is a rare disease caused by changes (mutations) in different genes coding for keratins (a type of proteins) expressed in keratinocyte (type of cells found in the outermost layer of the skin). (europa.eu)
This NHK basal cytotoxicity method uses human cells to estimate starting doses for in vivo acute oral toxicity tests, reducing the number of animals used for each test. (aavs.org)
The on-and-off switch is operated by transforming growth factor (TGF) β3 levels, which are undetectable in plasma and high in serum, and by TGFβ receptor (TβR) type II levels, which are low in epidermal cells and high in dermal cells. (rupress.org)
In cultured keratinocytes, CWO stimulated calcium signaling, resulting in calcineurin-dependent activation of nuclear factor of activated T cells (NFAT). (biorxiv.org)
ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. (rndsystems.com)
The skin findings in incontinentia pigmenti represent changes in the epidermal cells. (medscape.com)
Whereas the expression of syndecan-4 and glypican in intact skin was mostly in the pericellular regions of keratinocytes, the epidermal cells from the wound edge contained mostly intracellular PGs. (lu.se)
In the wound edge, syndecan-4 was predominantly expressed by epidermal basal layer cells. (lu.se)
Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)
SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)
Stem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. (bvsalud.org)

An environmental trigger is thought to evoke an inflammatory response and subsequent hyperproliferation of keratinocytes. (merckmanuals.com)

Our recent work focused on developing such markers and showed that necroptosis is activated in the severe drug-induced skin inflammatory disease Toxic Epidermal Necrolysis (TEN). (ukri.org)
Recently, I have identified alarmin-triggered necroptosis as a central mechanism in the pathophysiology of skin inflammation in Toxic Epidermal Necrolysis (TEN). (ukri.org)
The dermatologic manifestations of either toxic epidermal necrolysis or Stevens-Johnson syndrome may constitute a true emergency. (medscape.com)
Stevens-Johnson syndrome and toxic epidermal necrolysis are thought to be a single disease entity with common causes and mechanisms that is a severe cutaneous adverse reaction differentiated by the severity and extent of skin detachment. (medscape.com)
As with toxic epidermal necrolysis, medications are important inciting agents. (medscape.com)
[ 3 ] The mortality rate associated with Stevens-Johnson syndrome is much lower than the rate associated with toxic epidermal necrolysis, approaching 5% of cases. (medscape.com)
Together, Stevens-Johnson syndrome and toxic epidermal necrolysis may represent a spectrum of a single disease process. (medscape.com)
The initial skin lesions of Stevens-Johnson syndrome/toxic epidermal necrolysis are poorly defined, erythematous macules with darker, purpuric centers. (medscape.com)
As with toxic epidermal necrolysis, medications are important inciting agents, although Mycoplasma infections may induce some cases. (medscape.com)
The mortality rate is much lower than in toxic epidermal necrolysis, approaching 5% of cases. (medscape.com)

For example, seeding fibroblasts or keratinocytes results in faster vascularization due to growth factor secretion. (hindawi.com)

Topographical regulation of keratinocyte migration. (cellimagelibrary.org)
2D, 3D, and 1D fibrillar migration of human epidermal keratinocytes. (cellimagelibrary.org)
In this study, we report that human serum selectively promotes epidermal cell migration and halts dermal cell migration. (rupress.org)
In contrast, human plasma promotes dermal but not epidermal cell migration. (rupress.org)

Family history is common, and certain genes and human leukocyte antigens (Cw6, B13, B17) are associated with psoriasis. (merckmanuals.com)
Advances in the pathogenesis of psoriasis: From keratinocyte perspective. (msdmanuals.com)

Redox-cycling of phenols causes depletion of GSH, oxidative stress and cytotoxicity in normal human epidermal keratinocytes (NHEKS). (cdc.gov)
Redox cycling of phenol induces oxidative stress in human epidermal keratinocytes. (cdc.gov)
Oxidative stress and cytotoxicity of phenol and AMVN in human keratinocytes: evidence for enhanced lipid and thiol oxidation and antioxidant depletion. (cdc.gov)

In the present study, we investigated the effects of MPS in combination with clobetasol 17-propionate (CP) on tight junction (TJ) barrier function in human epidermal keratinocytes (HEKa) and 3D skin models.Methods: The expression of claudin-1, which is crucial for TJ barrier function in keratinocytes, and transepithelial electrical resistance (TEER) was measured in CP-treated human keratinocytes incubated with and without MPS. (journaltocs.ac.uk)
Non-invasive skin physiological parameters were assessed, including stratum corneum hydration, skin redness, surface pH, and basal transepidermal water loss (TEWL) as a parameter for epidermal barrier function. (journaltocs.ac.uk)
Results: Skin physiology tests revealed that DE in allergic patients deteriorates the epidermal barrier function, increases the surface pH and skin redness. (journaltocs.ac.uk)
I am a graduate student studying epidermal barrier function in atopic dermatitis. (eaaci.org)

In contrast, mice with keratinocyte-specific RIPK4 deletion during adult life remain viable. (ugent.be)
A spontaneously transformed human epithelial cell line developed from a long-term primary culture of human adult skin KERATINOCYTES. (bvsalud.org)

However, these methods have some drawbacks because the ECM substitutes contained exogenous materials such as bovine collagen, dead human allogenic dermis, or synthetic polymers [ 16 ]. (hindawi.com)

The typical histopathologic pattern of skin lesions is named interface dermatitis and characterized by a lymphocytic infiltrate and necroptotic keratinocytes at the dermo-epidermal junction. (frontiersin.org)
Lupus erythematosus (LE) skin lesions typically feature a histopathologic pattern termed interface dermatitis, defined by the presence of necroptotic keratinocytes and an epitheliotropic cytotoxic lymphocytic infiltrate at the dermo-epidermal junction ( 7 , 8 ). (frontiersin.org)

Mellerio also described a recently completed phase 3 trial with introduction of ex vivo gene-corrected keratinocytes, which has been associated with long-term improvements among patients with recessive dystrophic EB (RDEB). (medscape.com)

Genomic structure of keratinocyte transglutaminase. (wikipedia.org)

Previous reports have shown that VEGF overexpression in keratinocytes augments wound vascularization [ 13 ]. (hindawi.com)
Psoriasin (S100A7) was first identified for its overexpression in psoriatic keratinocytes (3), where it was found to be located to the nucleus and the cytoplasm (4). (medicaljournals.se)

Keratinocyte-Specific Ablation of RIPK4 Allows Epidermal Cornification but Impairs Skin Barrier Formation," JOURNAL OF INVESTIGATIVE DERMATOLOGY , vol. 138, no. 6, pp. 1268-1278, 2018. (ugent.be)

1999. Response of normal human keratinocytes to sulfur mustard (HD): Cytokine release using a non-enzymatic detachment procedure. (cdc.gov)

We fractionated the water-soluble proteins of royal jelly and identified one fraction (Fraction 2) that induced both proliferative and migratory effects on a human epidermal keratinocyte cell line. (biomedcentral.com)

We show here that the epithelial fusions observed in RIPK4 full knockout (KO) mice are E-cadherin dependent, as keratinocyte-specific deletion of E-cadherin in RIPK4 full KO mice rescued the tail-to-body fusion and fusion of oral epithelia. (ugent.be)

Apart from their protective functions, keratinocytes express adhesion molecules and cytokines, further suggesting an implication in skin innate immunity, tissue homeostasis, wound healing, cancer development, and skin-based gene-therapy. (3hbiomedical.com)

Both the innate stimuli, such as lipopolysaccharides and Poly inositol-cytosine and adaptive stimuli, that is, cytokine mix, were able to induce the extracellular release of NPM in immortalized keratinocytes and human skin fibroblasts in the absence of cytotoxicity. (frontiersin.org)
Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. (rndsystems.com)

The results showed that epi-oxyzoanthamine significantly decreased skin barrier damage, scratching responses, and epidermal hyperplasia induced by DNCB. (bvsalud.org)

Protein-glutamine gamma-glutamyltransferase K is a transglutaminase enzyme that in humans is encoded by the TGM1 gene. (wikipedia.org)
It was concluded that those afflicted, display a decrease in activity of the enzyme, as a result of a lessened amount of transcription of the human TGK gene. (wikipedia.org)
Evolution of transglutaminase genes: identification of a transglutaminase gene cluster on human chromosome 15q15. (wikipedia.org)
The epigenetic regulation of spatiotemporal gene expression is crucial for human development. (researchgate.net)

Our findings provide important insights into ZIKV tropism and subsequent antiviral signaling pathways that regulate ZIKV replication in human dermal fibroblasts and human epidermal keratinocytes. (korea.ac.kr)
America and the Caribbean Islands, despite a shorter and of detection was equal between the 2 compartments for 7 lower viremia in humans and a longer replication period (33%, 95% CI 15%-57%) of 21 patients (nos. (cdc.gov)

Cyclic tensile strain increases interactions between human epidermal keratinocytes and quantum dot nanoparticles. (ncsu.edu)

The deciphering of genes that regulate stemness remains an enigma that is only partially resolved, in particular for human skin. (cea.fr)

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. (rndsystems.com)
ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. (rndsystems.com)

In humans, receptor-interacting protein kinase 4 (RIPK4) mutations can lead to the autosomal recessive Bartsocas-Papas and popliteal pterygium syndromes, which are characterized by severe skin defects, pterygia, as well as clefting. (ugent.be)

Although the strategy of ICE is risk (HR) HPV infection in the etiology of beta human papillomavirus (HPV) types global, work is natural y focused on low- head and neck cancer. (who.int)

Keratinocytes are named after keratin, which is the most abundant protein in this cell. (3hbiomedical.com)
The specific cross linkages formed by keratinocyte transglutaminase are between n^ε-(γ-glutamyl)lysine residues which develop into isopeptide protein-protein linkages that adds to the stabilization of the cornified cell envelope. (wikipedia.org)
The robotic finger was first submerged in a solution of collagen, which is a fibrous protein, and human dermal fibroblasts, the two major components that make up human skin. (cbs58.com)
As an example of protein therapy, a completed phase I/II trial associated recombinant human collagen with wound healing and pain reduction in RDEB. (medscape.com)

Cerebral organoids model human brain development and microcephaly. (nature.com)
A TJ permeability assay, using Sulfo-NHS-Biotin as a tracer, was also conducted in a 3D skin model.Results: CP reduced claudin-1 expression and TEER in human keratinocytes, whereas MPS inhibited these CP-induced effects. (journaltocs.ac.uk)
We model central nervous system development using organoids, and select three histone modifications as proxies for dynamic epigenetic change and validate our findings in a primary developing human brain. (researchgate.net)
A model of reconstructed human epithelium, developed to test skin corrosion. (aavs.org)
Used in the study of skin corrosion and toxicology, EpiDermTM is a layered model of human-derived epidermal keratinocytes. (aavs.org)
We tested an essential-oil derivative, camphor white oil (CWO), for anti-tumor activity in a mouse model of keratinocyte-derived skin cancer. (biorxiv.org)
2021). research, from etiology or natural history aim to evaluate the role of infectious through global burden assessment to agents in human cancers through biolog- The overall strategy of ICE is to improve evaluation and model ing of the impact of ical and epidemiological studies. (who.int)

Collagen is a major component of human skin and has healing properties, according to a 2021 study. (cbs58.com)

However, to date, necroptosis has been poorly investigated in human diseases due to the lack of reliable biomarkers. (ukri.org)

Epidermal transglutaminase is the autoantigen, in humans, of dermatitis herpetiformis. (wikipedia.org)

Anatomy15

Organisms2

Diseases10

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes24

Disciplines and Occupations1

Information Science2

Named Groups1

Health Care1

Differentiation23

Vitro7

NHEK1

Proliferation6

HaCaT2

Impaired epidermal barrier function1

Epidermis9

Cells27

Hyperproliferation of keratinocytes1

Toxic Epidermal10

Fibroblasts or keratinocytes1

Migration4

Psoriasis2

Oxidative Stress3

Barrier function4

Adult2

Dermis1

Lesions2

Vivo1

Genomic1

Overexpression2

Cornification1

Normal1

Proteins1

Epithelia1

TISSUE1

Extracellular2

Skin barrier1

Gene4

Replication2

Increases1

Genes1

Receptors2

Mutations1

Infection1

Protein4

Model7

20211

Poorly1

Dermatitis1