Human epidermal keratin keratin 20. Medical search. Frequent questions

The local inflammatory reaction following perturbation of the epidermal barrier (either by exposure to a physical or chemical irritant) can appear within minutes to hours after the insult, and is believed to be initiated by the release of primary cytokines from keratinocytes [ 9 10 ]. (ispub.com)
These keratins are constitutively expressed in keratinocytes of the nail, palmoplantar skin, mucosa, and hair, leading to the manifestations of the disorder in these sites. (medscape.com)
Hair is made up of 95% keratin, synthesized by the epidermal keratinocytes. (naturalmedicinejournal.com)
Monoclonal Antibody Analysis of Keratin Expression in Epidermal Diseases: A 48- and 56-kdalton Keratin as Molecular Markers for Hyperproliferative Keratinocytes. (novodiax.com)
More recent skin substitutes combine epidermal and dermal layers by introducing fibroblasts and keratinocytes into an acellular matrix [ 5 ]. (hindawi.com)
This cell line has been known to retain a capacity fornormal differentiation up to multiple passages similar to normal human epidermal keratinocytes (NHEK), and hence offers a suitable model for keratinisation studies. (reprocell.com)
Psoriasis lesions are characterized by hyper-proliferation of epidermal keratinocytes associated with inflammatory cellular infiltrate in both dermis and epidermis. (springer.com)
Histologically, the dermatosis is characterized by hyperproliferation of keratinocytes, impaired epidermal barrier function at the sites of skin lesions, and skin infiltration by activated inflammatory cells [ 5 ]. (springer.com)
The thickness of the epidermis is about 0.1MM, mainly composed of two types of cells, keratinocytes and dendritic cells, and keratinocytes account for more than 80% of epidermal cells. (forimi.com)
In the process of differentiation, keratin is produced, and the proliferation of keratinocytes has a certain regularity. (forimi.com)
Keratinocytes develop from stem cells at the base of the epidermis and begin to produce and store the protein keratin. (innerbody.com)
Keratin makes the keratinocytes very tough, scaly and water-resistant. (innerbody.com)
The function of AQP5 in epidermal keratinocytes is currently unknown, but the diffuse NEPPK patient phenotype indicates that AQP5 has a role in the establishment and/or maintenance of the epidermal barrier, particularly in the highly specialised epidermis of the palms and soles (palmoplantar) which is exposed to increased levels of mechanical stress. (qmul.ac.uk)
In this research, we verified the effect of GMP on treatment for acne, including P.acnes suppression, anti-inflammation on human SZ-95 sebocytes and HaCaT keratinocytes, as well as beneficial effects on follicular hyperkeratosis. (cosmeticsbusiness.com)
Renews epidermal keratinocytes and prevents trans-epidermal water loss. (amareaesthetics.com)
To establish the skin barrier and the epidermal structure, keratinocytes undergo a tightly regulated process of differentiation. (medicaljournals.se)

This research can be extended to make it possible to define the dermal exposure to any hazardous chemical that reacts with keratin-rich epidermis, in either occupational or environmental exposure conditions. (cdc.gov)
Immunolocalization of Keratin Polypeptides in Human Epidermis Using Monoclonal Antibodies. (novodiax.com)
It is derived from cells within the epidermis that make keratin - the horny protein that makes up skin, hair and nails. (idahonewsupdates.com)
It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K10). (progen.com)
At about 8% of epidermal cells, melanocytes form the second most numerous cell type in the epidermis. (innerbody.com)
Langerhans cells are the third most common cells in the epidermis and make up just over 1% of all epidermal cells. (innerbody.com)
In addition, the gene that encodes psoriasin is positioned within the epidermal differentiation complex (EDC) (10-12), a gene cluster that contains several genes of importance in the terminal differentiation of the human epidermis (10, 11). (medicaljournals.se)
Human and in the epidermis, form the major protective barrier that pre- animal studies of atopic dermatitis have identified skin epithelial vents loss of water and protects against foreign substances. (cdc.gov)

In the present study, the effect of very low nanomolar concentrations of Sb(III), able to mimicking chronic human exposure, was evaluated in 3T3-L1 murine cells during the differentiation process. (bvsalud.org)
1-5 Accumulated knowledge in the past decade has demonstrated the importance of tear components such as epidermal growth factor (EGF) and vitamin A in the regulation of proliferation, differentiation, and maturation of the ocular surface epithelium. (bmj.com)
After 21 days, cell differentiation is noticeable within the construct, indicated by intense pink staining of the cell cytoplasm due to increased keratin production, anucleation and flattening of cell morphology. (reprocell.com)
This is further supported by the expression of late differentiation markers (keratin 10 and involucrin) which occur towards the air exposed surface of the culture with increasing expression from 7-21 days (Figure 3). (reprocell.com)
Ks 2.342.7.4 represents an excellent marker to study terminal epidermal differentiation. (progen.com)
The modulation of epidermal differentiation by retinoids led to its use in premalignant lesions, such as actinic keratoses (Verschoore et al. (medscape.com)
Psoriasin, which is highly expressed in psoriasis, is encoded by a gene located within the epidermal differentiation complex. (medicaljournals.se)
In psoriasis, the differentiation process of the epidermal cells is disturbed. (medicaljournals.se)

Keratins are heteropolymeric structural proteins which form the intermediate filament. (icbinhibitor.com)
Newly synthesized proteins in the endoplasmic reticulum (ER) are sorted by coat protein complex II (COPII) at the ER exit site en route to the Golgi. (bvsalud.org)
Keratins are key structural proteins that form the cytoskeleton of epithelial cells. (medscape.com)
These keratin spindles are arranged in a hierarchy, starting with the smallest structure, the keratin proteins, to the largest and final structure, the cortex. (naturalmedicinejournal.com)
Keratin-associated proteins are initially produced in the cytoplasm among keratin bundles that accumulate in cortical and cuticle cells of hair. (naturalmedicinejournal.com)
Keratin ( / ˈ k ɛr ə t ɪ n / [1] [2] ) is one of a family of structural fibrous proteins also known as scleroproteins . (wikipedia.org)
The health and appearance of our skin, hair and nails depends on the health of several structural proteins they share, namely collagen, keratin and elastin. (lifeextension.com)
The appearance and integrity of our skin, hair, and nails are dependent upon a framework of structural proteins, especially keratin, collagen, and elastin. (lifeextension.com)
To aid our understanding of the physiology of the epidermal barrier, we perform cell and molecular biology studies on proteins identified in association with rare skin diseases that manifest as barrier defects. (qmul.ac.uk)
Implanting something made from naturally synthesized proteins, such as a keratin based implant, has the potential to be recognized as natural tissue by the body. (calflameblog.com)
The human body is armed with the proper binding proteins and enzymatic machinery to metabolize these retinoids appropriately (Prystowsky, 2001). (medscape.com)
A cor- support an important role for skin exposure in initiating and nified layer of lipids and proteins, such as keratins and filaggrin driving Th2-like immune responses and asthma. (cdc.gov)

Keratin, type I cytoskeletal 17 is a protein that in humans is encoded by the KRT17 gene. (wikipedia.org)
Storage Buffer: 20 mM Tris-Borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% sodium azide, pH 7.5. (icbinhibitor.com)
Keratin intermediate filament assembly begins with the pairing of a type I keratin protein and type II keratin protein to form an alpha helical heterodimer. (medscape.com)
The basic protein structure of a keratin filament consists of an alpha-helical rod that is divided into 4 domains (1A, 1B, 2A, 2B) connected together by nonhelical linkers (L1, L12, L2). (medscape.com)
The keratin protein is a helicoidal group of cysteine-containing protein complexes with primary, secondary, and tertiary structures held together by peptide bonds, disulfide bonds, and hydroxide bonds. (naturalmedicinejournal.com)
Spider silk is classified as keratin, [9] although production of the protein may have evolved independently of the process in vertebrates. (wikipedia.org)
The new term corneous beta protein (CBP) has been proposed to avoid confusion with α-keratins. (wikipedia.org)
This project aimed to develop a noninvasive procedure for collection of an epidermal tissue sample and to detect the deposition and penetration of a multifunctional acrylates into the stratum corneum and the formation of acrylate-keratin protein adducts as a biomarker of exposure. (cdc.gov)
Although a reliable and reproducible tape-stripping procedure was developed for tripropylene glycol diacrylate (TPGDA) exposed human skin, synthesis of a TPGDA-protein haptenic complex failed to produce epitope specific antisera for quantification. (cdc.gov)
This method can be used for isolation of keratin protein for identification and quantification of adducts by an enzyme-linked immunoabsorbent assay (ELISA). (cdc.gov)
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. (abcam.co.jp)
These "horns" are not really true horns, but are epidermal derivatives, composed of a solid mass of thickly matted hair-keratin, a hair protein-that grows from the skull without skeletal support. (newworldencyclopedia.org)
The naming convention for these keratins follows that for protein structures: alpha keratin is helical and beta keratin is sheet-like. (calflameblog.com)
Differentially regulated genes were validated at the level of gene and protein expression, with genes identified as estrogen-regulated in human confirmed as estrogen-dependent in young estrogen depleted mice in vivo . (biomedcentral.com)

Mutations in signalling pathways affect the epidermal growth factor receptor, RAS, Fyn, and p16INK4a signalling. (idahonewsupdates.com)

Keratin is insoluble in water, thus conferring impermeability and protection for the hair. (naturalmedicinejournal.com)
Keratin is extremely insoluble in water and organic solvents. (wikipedia.org)

It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. (wikipedia.org)
Keratin monomers assemble into bundles to form intermediate filaments , which are tough and form strong unmineralized epidermal appendages found in reptiles , birds , amphibians , and mammals . (wikipedia.org)

Human skin has four functions of barrier, regulation, self-stabilization and metabolism. (forimi.com)
My research is centred around understanding epidermal barrier function, primarily through the identification of the underlying cause of rare inherited skin disorders, coupled with functional studies to aid understanding of both the underlying disease mechanism as well as the normal biology of the skin. (qmul.ac.uk)
epidermal barrier pulmonary immune diseases. (cdc.gov)

Keratin 10 (mouse monoclonal), Ki67 (rabbit polyclonal) and involucrin (mouse monoclonal) expression of HaCaT cells cultured on Alvetex Scaffold in 12-well inserts (AVP005) by immunohistochemical analysis of 4 % formaldehyde fixed, paraffin embedded sections (secondary antibodies, Alexa Fluor 488 donkey, anti-mouse and Alexa Fluor 488 goat, anti-rabbit). (reprocell.com)

[ 4 ] Pachyonychia congenita type 2, or the Jackson-Lawler type (MIM entry 167210), was attributed to mutations in keratin 6B (KRT6B) or keratin 17 (KRT17) and could be distinguished from type 1 by the development of natal teeth, widespread steatocystomas, and occasionally pili torti. (medscape.com)
The mutations in pachyonychia congenita are found in the genes encoding keratin 6A (KRT6A), keratin 16 (KRT16), keratin 6B (KRT6B), and keratin 17 (KRT17). (medscape.com)

In addition, a reliable and reproducible tape-stripping procedure for sampling and isolation of naphthalene-keratin adducts (antigen) from the stratum corneum under defined laboratory conditions was developed and tested on human volunteers exposed to jet fuel instead of TPGDA. (cdc.gov)

We also provide a summary of several genes abundantly or uniquely expressed in the human corneal epithelium, namely clusterin, keratin 3, keratin 12, aldehyde dehydrogenase 3 (ALDH3), troponin-I fast-twitch isoform, ßig-h3, cathepsin L2 (cathepsin V), uroplakin Ib, and Ca2+-activated chloride channel. (cardiff.ac.uk)

The subsequent wound healing processes aim to restore dermal and epidermal integrity and functionality. (biomedcentral.com)

Pachyonychia congenita is a rare genodermatosis due to mutations in one of four keratin genes. (medscape.com)
Pachyonychia congenita type 1, or the Jadassohn-Lewandowsky type (Mendelian Inheritance in Man (MIM entry 167200), was attributed to mutations in genes encoding keratin 6A (KRT6A) or keratin 16 (KRT16) and constituted the most common form of the disorder. (medscape.com)
Fifty four different keratin genes have been identified. (medscape.com)
Using the source a bit more to explain what each gene and each zone of genes mean will be helpful, as we currently have no particular examples of a hair keratin. (wikipedia.org)
The human genome encodes 54 functional keratin genes , located in two clusters on chromosomes 12 and 17. (wikipedia.org)

As with most other keratin disorders, most mutations in pachyonychia congenita occur in these highly conserved helix boundary domains at the end of the rod domain. (medscape.com)
Hafner C, van Oers JM, Vogt T, et al: Mosaicism of activating FGFR3 mutations in human skin causes epidermal nevi. (karger.com)
Rothnagel JA, Dominey AM, Dempsey LD, et al: Mutations in the rod domains of keratins 1 and 10 in epidermolytic hyperkeratosis. (karger.com)

Inhibition of Xfoxi1a activities by injection of antisense oligonucleotides leads to suppression of non-CNS ectodermal markers (e.g. keratin ) and expansion of the anterior expression domain of the CNS marker Sox2 . (biologists.com)
Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. (neobiotechnologies.com)

Each hair derives from an epidermal invagination called the hair follicle, which possesses a terminal hair bulb, located in the dermis or hypodermis, from which the hair shaft grows. (hsimm.ru)

However, these methods have some drawbacks because the ECM substitutes contained exogenous materials such as bovine collagen, dead human allogenic dermis, or synthetic polymers [ 16 ]. (hindawi.com)
Fortunately, lifestyle changes and targeted nutritional interventions-particularly collagen peptides, solubilized keratin, and Polypodium leucotomos extract-can support skin, hair and nails from the inside out. (lifeextension.com)
Bulletproof Collagen Peptides are the best collagen peptides because they deliver 20 grams of collagen per serving, and they're hydrolyzed to make digestion and absorption easier. (look-closer.net)

Briefly, the model utilizes non-transformed normal primary human esophageal fibroblasts and epithelial cells grown within a porcine-derived acellular esophageal scaffold. (docksci.com)
Keratin also protects epithelial cells from damage or stress. (wikipedia.org)
For example, mouse thymic epithelial cells react with antibodies for keratin 5, keratin 8, and keratin 14. (wikipedia.org)
The additive effect of human serum for cultured conjunctival epithelial cells showed significant MUC-1 upregulation on the cell surface. (bmj.com)
Finally, in situ hybridization analyses revealed the presence of RP11-295G20.2 in the nuclei of corneal epithelial cells, but not in the stromal cells of the human cornea, while RP11-322M19.1 was present in epithelial and non-epithelial cells. (molvis.org)
This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. (neobiotechnologies.com)
Human Papillomavirus (HPV) Infection Human papillomavirus (HPV) infects epithelial cells. (msdmanuals.com)

Epidermal nevi(EN)represent a heterogeneous group of mosaic skin lesions frequently following the lines of Blaschko. (karger.com)
Warts are common, benign, epidermal lesions caused by human papillomavirus infection. (msdmanuals.com)

A natural, highly purified and concentrated blend of monogalactosylceramide, cholesterol and phospholipids that are analogous to human epidermal lipids. (amareaesthetics.com)

Duct portions of sebaceous glands are composed of stratified squamous epithelium that is continuous with the hair cat and epidermal surface. (hsimm.ru)

[11] Recent scholarship has shown that sauropsid β-keratins are fundamentally different from α-keratins at a genetic and structural level. (wikipedia.org)

RNA and DNA in situ hybridization probes for 25 commensal β-HPVs revealed a significant reduction in viral activity and load in human skin cancer compared with the adjacent healthy skin, suggesting a strong immune selection against virus-positive malignant cells. (nature.com)
Consistently, E7 peptides from β-HPVs activated CD8 + T cells from unaffected human skin. (nature.com)
Fig. 3: Reduced β-HPV transcripts in skin cancer cells and presence of β-HPV-specific CD8 + T cells in healthy human skin indicates a selective pressure by antiviral immunity against malignant cells with active HPV. (nature.com)
A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture. (hindawi.com)
Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. (progen.com)
Finally, Merkel cells make up less than 1% of all epidermal cells but have the important function of sensing touch. (innerbody.com)
Hairs are long, filamentous projections consisting of dead keratini-zed epidermal cells. (hsimm.ru)

Along the dorsoventral (DV)axis, the ectoderm becomes subdivided into the dorsal (CNS or neural plate),intermediate (e.g. presumptive neural crest, placodes and cement gland) and ventral (epidermal) ectoderm. (biologists.com)
In contrast to the CNS, relatively little is known about the AP specification of the non-CNS (intermediate and epidermal) ectoderm. (biologists.com)
The tetramers subsequently aggregate to form larger order polymers that give rise to a keratin intermediate filament. (medscape.com)
Keratins (also described as cytokeratins ) are polymers of type I and type II intermediate filaments that have been found only in chordates ( vertebrates , amphioxus , urochordates ). (wikipedia.org)

Happle R, Rogers M: Epidermal nevi. (karger.com)
Waltz KM, Helm KF, Billingsley EM: The spectrum of epidermal nevi: a case of verrucous epidermal nevus contiguous with nevus sebaceus. (karger.com)
Solomon LM, Esterly NB: Epidermal and other congenital organoid nevi. (karger.com)
Epidermal nevi, neoplasms, and cysts. (medlineplus.gov)

The mab does not react with palate keratin K76. (progen.com)

This gene is located within a cluster of keratin family members on chromosome 17q21. (icbinhibitor.com)
The neutral-basic keratins are encoded on chromosome 12 (12q13.13). (wikipedia.org)
The acidic keratins are encoded on chromosome 17 (17q21.2). (wikipedia.org)

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 40kDa (CK19). (neobiotechnologies.com)

Expression of keratin 5 as a distinctive feature of epithelial and biphasic mesotheliomas. (novodiax.com)
Using an Affymetrix microarray-based approach we show that the differences in gene expression between male elderly and young human wounds are almost exclusively estrogen regulated. (biomedcentral.com)
Since the major variable contributing to chronic wounds in humans is being an aged male [ 4 , 5 ], our initial approach was to compare acute wound gene expression between young and old male human subjects via Affymetrix microarray. (biomedcentral.com)

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model. (docksci.com)
These epidermal and connective tissue layers protect against mechanical and chemical harm and repel pathogenic invaders [ 19 ]. (biomedcentral.com)
Classification of natural and synthetic fibres (Jawaid and Khalil 2011 - With Permission) Table 1 shows mechanical properties of different types of natural fibers for composite applications as compared with human tissue. (calflameblog.com)

The model also facilitates extended culture periods not achievable with conventional 2D cell culture, enabling, inter alia, the study of the impact of repeated exposure of a mature epithelium to the agent of interest for up to 20 days. (docksci.com)
Keratin concentration as well as naphthalene concentration (as a marker for jet fuel exposure) from the adhesive tape strips were determined. (cdc.gov)

Compared to the hairy skin covering the majority of the human body, palmoplantar skin found on our palms and soles is highly specialized to withstand increased levels of mechanical stress. (qmul.ac.uk)

A model of the human esophageal mucosa has been developed in the MacNeil laboratory which, unlike conventional 2D cell culture systems, recapitulates the cell-cell and cell-matrix interactions present in vivo and produces a mature, stratified epithelium similar to that of the normal human esophagus. (docksci.com)
An appreciation of the biological characteristics of the human ocular surface epithelium affords us a great insight into the physiology of the human ocular surface in health and disease. (cardiff.ac.uk)
Here, we review five important aspects of the human ocular surface epithelium. (cardiff.ac.uk)

6 Keratin is especially rich in cysteine, which is a sulfur-containing amino acid capable of forming disulfide bonds between molecules, thus adding strength and elasticity to the entire structure (Figure 1). (naturalmedicinejournal.com)
This successful demonstration of an antigenic epitope using this method provides a model for synthesis of an acrylated cysteine for synthesis of an antigenic keratin-lor keratin-lO peptide for preparation of an immunogenic complex. (cdc.gov)

Despite the fact that a common and clear definition of "berries" is not shared among different scientific areas, the phytochemical pattern of these fruits is mainly characterized by anthocyanins, flavanols, flavonols, and tannins, which showed antioxidant and anti-inflammatory properties in humans. (mdpi.com)

The cortex is in the center of each hair fiber and consists of long, tightly packed keratin spindles stabilized by disulfide bonds. (naturalmedicinejournal.com)

The stability of the serum was assessed by measuring the concentration of EGF, TGF-β, and vitamin A in fresh serum samples, and after preservation in the freezer (−20°C) and refrigerator (4°C) for up to 3 months. (bmj.com)

HaCaT is an immortalised human adult keratinocyte cell line. (reprocell.com)

Keratin 17 is a type I cytokeratin. (wikipedia.org)
They are classified based on their biochemical properties into either type I (K9-K28, K31-K40) or type II keratins (K1-K8, K71-K86). (medscape.com)
Alpha-keratin (α-keratin) is a type of keratin found in vertebrates . (wikipedia.org)
Nevus Marginatus: A Distinct Type of Epidermal Nevus or Merely a Variant of Nevus Sebaceus? (karger.com)
Herein we report on a 35-year-old man with a rather unusual type of EN. (karger.com)
Paller AS, Syder AJ, Chan YM, et al: Genetic and clinical mosaicism in a type of epidermal nevus. (karger.com)

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here . (abcam.com)

The horns of the impala are made of keratin covering a core of bone . (wikipedia.org)

Hufbauer, M. & Akgül, B. Molecular mechanisms of human papillomavirus induced skin carcinogenesis. (nature.com)

Anatomy5

Organisms2

Diseases2

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes3

Technology, Industry, Agriculture1

Information Science4

Named Groups1

Health Care4

Keratinocytes16

Epidermis8

Differentiation8

Proteins12

Protein14

Growth factor r1

Insoluble in water2

Appendages2

Barrier3

Monoclonal1

KRT172

Stratum corneum1

Corneal epithelium1

Dermal1

Genes5

Mutations3

Markers2

Hair follicle1

Collagen3

Epithelial cells7

Lesions2

Lipids1

Squamous1

Structural1

Cells8

Intermediate4

Nevi4

React1

Chromosome3

Acidic1

Expression3

Tissue3

Exposure2

Palmoplantar skin1

Epithelium3

Cysteine2

Anti-inflammatory1

Tightly1

Concentration1

Keratinocyte cell line1

Type6

Antibody1

Horns1

Papillomavirus1