• This study aims to clarify the time-dependent molecular pathways by which TMAO mediates endothelial dysfunction through transcriptomics and metabolomics analyses in human microvascular endothelial cells (HMEC-1). (nature.com)
  • SNAI2, an angiogenesis-related factor, was highly expressed under the high glucose condition and also led to viability and migration of human brain microvascular endothelial cells (HBMECs) [ 13 ]. (hindawi.com)
  • The aim of this study was to assess whether activation of nrf2 by sulforaphane in human microvascular endothelial cells prevents metabolic dysfunction in hyperglycemia. (diabetesjournals.org)
  • Studies evaluating the pharmacological effects of defibrotide on endothelial cells (ECs) were conducted primarily in the human microvascular endothelial cell line. (medscape.com)
  • Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure. (cdc.gov)
  • Endothelial dysfunction is a critical initiating factor contributing to cardiovascular diseases, involving the gut microbiome-derived metabolite trimethylamine N-oxide (TMAO). (nature.com)
  • The discrepancies between studies on TMAO-induced endothelial dysfunction may be attributed to different cell types and animal models used, including different metabolic backgrounds, as well as to distinct TMAO treatment concentrations and durations. (nature.com)
  • Recently, endothelial dysfunction (ED) has been demonstrated in these patients, but the mechanisms remain elusive. (mdpi.com)
  • Endothelial dysfunction during diabetes has been previously reported to be at least in part attributed to increased oxidized low‑density lipoprotein (oxLDL) levels mediated by high glucose (HG) levels. (spandidos-publications.com)
  • These results suggest that rosmarinic acid can exert a protective effect against oxLDL‑mediated endothelial dysfunction under HG conditions by reducing the interaction between monocytes and ECs in addition to preventing monocyte diapedesis. (spandidos-publications.com)
  • A number of studies have previously suggested that vascular endothelial dysfunction caused by increased junctional permeability is a key event in the development and progression of atherosclerosis and atherosclerotic cardiovascular diseases ( 3 , 4 ). (spandidos-publications.com)
  • Chronic metabolic disorders, such as hyperglycemia and dyslipidemia, promote endothelial dysfunction and eventually leakage, resulting in increased monocyte diapedesis ( 5 - 7 ). (spandidos-publications.com)
  • Endothelial dysfunction during diabetes is closely associated with increased oxidized low-density lipoprotein (oxLDL) levels mediated by high glucose (HG) conditions ( 8 ). (spandidos-publications.com)
  • Systemic autoimmune diseases, such as rheumatoid arthritis (RA), exhibit accelerated atherosclerosis (AS) [ 1 - 4 ] as a consequence of endothelial dysfunction, leading to higher incidence of cardiovascular (CV) disease (at least 2-fold enhanced CV risk) and premature and higher mortality [ 5 , 6 ]. (hindawi.com)
  • SAA was shown to exhibit causal properties in AS, as a consequence of endothelial dysfunction (elevating tissue factor, as well as a variety of cytokines/chemokines) and early lesions (biglycan synthesis) [ 15 ] to plaque destabilization by inducing matrix metalloproteinases [ 16 ]. (hindawi.com)
  • CONCLUSIONS- We conclude that activation of nrf2 may prevent biochemical dysfunction and related functional responses of endothelial cells induced by hyperglycemia in which increased expression of transketolase has a pivotal role. (diabetesjournals.org)
  • It is indicated for adults and children with hepatic veno-occlusive disease (VOD), also known as sinusoidal obstruction syndrome (SOS), with renal or pulmonary dysfunction following hematopoietic stem cell transplantation (HSCT). (medscape.com)
  • Endothelial-Derived Extracellular Vesicles Induce Cerebrovascular Dysfunction in Inflammation. (open.ac.uk)
  • This has also been reported to work well in bronchial epithelial cells (UBH16E) and THP-1 cells. (novusbio.com)
  • For over 50 years, glucose has been recognised to cross the lung epithelial barrier and be transported by lung epithelial cells. (ersjournals.com)
  • In primary cultures of human bronchial epithelial cells (HBECs) with 16.6±0.4 mM glucose in the basolateral medium, ASL glucose was 2.2±0.5 mM [ 11 ]. (ersjournals.com)
  • Aquaporin-5 (AQP5) is a water-specific channel located on the apical surface of airway epithelial cells. (plos.org)
  • In contrast, overexpression of AQP5 increased assembly of microtubules, with evidence of increased MT stability, and promoted the formation of long straight microtubules in the apical domain of the epithelial cells. (plos.org)
  • Aquaporin-5 (AQP5) is a water-specific channel located on the apical membrane of epithelial cells in several sites in mammals, including corneal and pancreatic epithelium, secretory cells in salivary and lacrimal glands, and airway submucosal glands, bronchial epithelium and type I pneumocytes of the respiratory tract. (plos.org)
  • Airway epithelial cells can dynamically regulate paracellular permeability in response to both physiologic and pathologic stimuli [6] , [11] . (plos.org)
  • AQP5 can regulate paracellular permeability in primary airway epithelial cells [6] . (plos.org)
  • In airway epithelial cells, altered AQP5 abundance is associated with changes in actin organization and in desmoplakin localization, which could contribute to the changes in paracellular permeability. (plos.org)
  • The neutralizing antibody response to PC-positive DBs was superior to that of PC-negative DBs, as tested on fibroblasts, epithelial cells, and endothelial cells and for both animal species used. (asm.org)
  • In addition, at 48H, BPs linked to inflammatory phenotypes were activated (confirming KEGG results), while BPs linked to extracellular matrix (ECM) structural organisation, endothelial cell proliferation, and collagen metabolism were repressed. (nature.com)
  • VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
  • It was postulated that cell proliferation may also be enhanced by fibrinogen binding to CD54 ( 21 ). (iiarjournals.org)
  • We demonstrate that UCN-01, at concentrations lower than those necessary to inhibit cancer cell growth, inhibit proliferation of human endothelial cells in vitro. (karger.com)
  • We conclude that UCN-01, at clinically relevant concentrations, exerts an anti-neovascularization effect by blocking two important steps in vessel formation: (1) the response of cancer cells to hypoxia, and (2) endothelial cell proliferation. (karger.com)
  • In addition, cell proliferation and angiogenesis will be stimulated, enabling better oxygenation of the tissue. (bmj.com)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • Processes involved in atherosclerosis include coagulation, inflammation, lipid metabolism, intimal injury, and smooth muscle cell proliferation (see the image below). (medscape.com)
  • In addition, oxLDL‑stimulated ECs under HG conditions exhibited increased phosphorylated VE‑cadherin protein levels and decreased ZO‑1 protein expression levels compared with those in untreated ECs, suggesting increased endothelial permeability. (spandidos-publications.com)
  • Knockdown of miR-10 led to premature truncation of intersegmental vessel growth (ISV) in the trunk of zebrafish larvae, while overexpression of miR-10 promoted angiogenic behavior in zebrafish and cultured human umbilical venous endothelial cells (HUVECs). (ca.gov)
  • They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis). (wikipedia.org)
  • Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
  • Methods and Results: We show that the highly conserved microRNA family encoding miR-10 regulates the behavior of endothelial cells during angiogenesis by positively titrating pro-angiogenic signaling. (ca.gov)
  • The Proteome Profiler Human Angiogenesis Array Kit is a membrane-based sandwich immunoassay. (rndsystems.com)
  • Since hypoxia activates hypoxia-inducible factor (HIF-1)-dependent transcription in cancer cells that, in a paracrine fashion, drive tumor angiogenesis, we investigated the effects of UCN-01 on HIF-1-responsive promoter constructs. (karger.com)
  • HPAEC-BMI1 are immortalized human pulmonary endothelial cells that can be used in angiogenesis studies, drug discovery and development, and high throughput screening. (atcc.org)
  • We show that a microRNA called miR-10 regulates the behavior of endothelial cells during blood vessel formation by encouraging signals that promote blood vessel growth. (ca.gov)
  • The lack of binding of mIgG to hFcRn explains why murine antibodies are rapidly removed from the circulation in humans, despite long half-life in mice 32 . (nature.com)
  • Cell tracking using MRI, PET and qPCR in a murine model of limb ischemia demonstrated that hESC-ECP were detectable up to day 7 following injection. (ed.ac.uk)
  • Evaluation of the toxicity of welding fume particles on murine macrophage (RAW 264.7) and human placental (HTR- 8/SVneo) cell lines. (cdc.gov)
  • Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. (nature.com)
  • Cell viability was assessed using the Cell Counting Kit‑8 (CCK‑8) assay. (spandidos-publications.com)
  • The qRT-PCR was used to measure the expression levels of the miR-429 in the serums of the patients or cells, and CCK-8, wound healing assay, and tube formation assay were used to observe the effects of miR-429 on the phenotype of HBMECs. (hindawi.com)
  • The MCP-1 and vWF activity in cell supernatants was measured with an enzyme-linked immunosorbent assay (ELISA) kit. (iiarjournals.org)
  • These luciferase expressing cells can be used as a target cancer cell for in vitro killing assay by CD20 CAR-T cells. (atcc.org)
  • Genetic experiments in zebrafish revealed that miR-10 functions, in part, by directly regulating the level of a protein called FLT1, which inhibits the behavior of endothelial cells that promotes new blood vessel growth. (ca.gov)
  • A human-derived neurovascular unit in vitro model to study the effects of cellular cross-talk and soluble factors on barrier integrity. (open.ac.uk)
  • The neurovascular unit (NVU) contains endothelial cells joined by tight junctions that shield the brain from the blood via the blood brain barrier (BBB). (lu.se)
  • Tissue engineering, also known as regenerative medicine, is a field aimed at integrating advances in stem cell biology and materials science to grow transplantable replacement tissues and organs. (sciencedaily.com)
  • While tissue engineers have found dozens of ways to coax stems cells into forming specific kinds of cells and tissues, they still cannot grow tissues with vasculature -- capillaries and the larger blood vessels that can supply the tissues with life-giving blood. (sciencedaily.com)
  • Glaucoma leads to the loss of retinal ganglion cells and their axons but also to tissue remodelling which involves both the optic nerve head and the retina. (molvis.org)
  • Hundreds of thousands of those organ breeze blocks are mixed into a slurry and compacted, at low temperature, to form a matrix of cells with roughly the density of human tissue. (cosmosmagazine.com)
  • Immunohistochemistry-Paraffin: MORF4L2 Antibody [NBP2-47370] - Staining of human colon tissue. (novusbio.com)
  • The idea is that companies can use the printed tissue to test whether experimental drugs are safe for human livers. (popsci.com)
  • The result is a tissue that's supposed to react more like human livers do to experimental drugs. (popsci.com)
  • In the periapical granuloma cases, mast cells were found in eight cases (50%), located in the granulation tissue. (bvsalud.org)
  • Mast cells were detected in both cysts and periapical granuloma, located in the capsule and granulation tissue, respectively. (bvsalud.org)
  • Mast cells are cells that reside in the connective tissue and contain a large number of granules, rich in histamine, heparin, chymase, serotonin, and also cytokines. (bvsalud.org)
  • In a paper published online in the journal Biomaterials Science , a team from the laboratories of Rice bioengineer Jordan Miller and Baylor College of Medicine biophysicist Mary Dickinson showed how to use a combination of human endothelial cells and mesenchymal stem cells to initiate a process called tubulogenesis that is crucial to the formation of blood-transporting capillaries. (sciencedaily.com)
  • It is also expressed on mesenchymal stem cells, erythroid precursors, activated macrophages, pre-B cells, and some tumor cells and cell lines including U937 cells. (bdbiosciences.com)
  • The clinical-grade cell line RC11 was used to generate hESC-ECP which were identified as mostly endothelial (60% CD31+/CD144+), with the remainder of the subset expressing various pericyte/mesenchymal stem cell markers. (ed.ac.uk)
  • ATCC offers well-characterized cell lines, organoids, primary and hTERT-immortalized cells, iPSCs, and CRISPR/Cas9 genome-edited cells that support oncology drug development. (atcc.org)
  • ATCC (American Type Culture Collection) is a nonprofit, global biological resource center and standards organization and the leading developer and supplier of authenticated cells lines and microorganisms. (atcc.org)
  • U937 cells (ATCC, Cat No. CRL-1593.2) were stained with PE Mouse anti-Human CD105 antibody (Cat. (bdbiosciences.com)
  • Furthermore, both the mouse and human receptors bind more strongly to mouse serum albumin (MSA) than to HSA. (nature.com)
  • PCS (n = 17), PCS/CFS (n = 13), and healthy controls (HC, n = 14) were screened for serum anti-endothelial cell autoantibodies (AECAs) and dysregulated cytokines. (mdpi.com)
  • Altered functional and molecular responses of endothelial cells treated with serum collected from rats exposed to different welding fumes. (cdc.gov)
  • We report that, in addition to direct inhibitory effects on endothelial cell growth, UCN-01 abrogates hypoxia-mediated transactivation of HIF-1-responsive promoters in a prostate cancer cell line. (karger.com)
  • The severity of hypoxia determines whether cells become apoptotic or adapt to hypoxia and survive. (bmj.com)
  • Cells adapt to this environmental stress, so that after repeated periods of hypoxia, selection for resistance to hypoxia induced apoptosis occurs. (bmj.com)
  • Mammalian cells have developed a range of adaptations to survive acute and even prolonged hypoxia. (bmj.com)
  • Hypoxia reduces the ability of a cell to maintain its energy level, because less ATP will be obtained from glycolysis than from oxidative phosphorylation. (bmj.com)
  • 3- 5 Therefore, during severe hypoxia or anoxia, the cell initiates a cascade of events that leads to apoptotic cell death, thereby preventing the accumulation of cells with hypoxia induced mutations. (bmj.com)
  • 7 Severe hypoxia in the presence of energy stimulates cells to undergo apoptosis, whereas oxygen levels above 0.5% prevent cell death. (bmj.com)
  • However, changes in cell signalling in response to hypoxia that are mediated by RGS5 stroke. (lu.se)
  • Therefore, the present study aimed to elucidate the mechanisms involved in oxLDL‑induced endothelial permeability and monocyte diapedesis under HG conditions, in addition to the potential effects of rosmarinic acid. (spandidos-publications.com)
  • Furthermore, monocyte transmigration through the endothelial monolayer was significantly increased by oxLDL treatment under HG conditions. (spandidos-publications.com)
  • Subsequently, rosmarinic acid treatment significantly reversed oxLDL‑induced overexpression of adhesion molecules and monocyte‑EC adhesion, oxLDL‑induced endothelial junction hyperpermeability and monocyte transmigration through the endothelial monolayer under HG conditions, in a dose‑dependent manner. (spandidos-publications.com)
  • We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
  • Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
  • miR-10 Regulates the Angiogenic Behavior of Zebrafish and Human Endothelial Cells by Promoting VEGF Signaling. (ca.gov)
  • We found that miR-10 functions, in part, by directly regulating the level of fms-related tyrosine kinase 1 (FLT1), a cell-surface protein that sequesters VEGF, and its soluble splice variant sFLT1. (ca.gov)
  • Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
  • Various signaling pathways control the behavior of endothelial cells-the cells that line the interior surface of blood vessels. (ca.gov)
  • Various signaling pathways control the behavior of endothelial cells, but their post-transcriptional dose-titration by miRNAs is poorly understood. (ca.gov)
  • This reporter cell line is useful for monitoring the activity of IFNγ-induced GAS signal transduction pathways. (atcc.org)
  • Endothelial cell (EC) monolayers located in the inner lining of blood vessels serve as a semipermeable barrier between circulating blood and surrounding tissues. (rsc.org)
  • In vitro studies in resistive human lung epithelial cell monolayers grown at an air-liquid interface have supported these in vivo observations. (ersjournals.com)
  • Stem Cell Rev. 2 (2): 93-102. (wikipedia.org)
  • The work is an important step with fragile endothelial cells (ECs) made from "induced pluripotent stem cells," or iPSCs, a type of cell that can potentially be made from the cells of any human patient. (sciencedaily.com)
  • Incidence, survival and risk factors for the development of veno-occlusive disease in pediatric hematopoietic stem cell transplant recipients. (medscape.com)
  • A prospective survey on incidence, risk factors and therapy of hepatic veno-occlusive disease in children after hematopoietic stem cell transplantation. (medscape.com)
  • Stem cell transplantation in children with infantile osteopetrosis is associated with a high incidence of VOD, which could be prevented with defibrotide. (medscape.com)
  • They start by making "organ building blocks" from human stem cells, which they chemically cajole into becoming mini hearts and brains. (cosmosmagazine.com)
  • A hydrogel model of the human blood-brain barrier using differentiated stem cells. (open.ac.uk)
  • Pluripotent stem cell-derived differentiated endothelial cells offer high potential in regenerative medicine in the cardiovascular system. (ed.ac.uk)
  • With the aim of translating the use of a human stem cell-derived endothelial cell product (hESC-ECP) for treatment of critical limb ischemia (CLI) in man, we report a GMP-compatible protocol and detailed cell tracking and efficacy data in multiple pre-clinical models. (ed.ac.uk)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • In polarized epithelium, adhesion between adjacent cells is mediated by intercellular junctions, namely, tight junctions, adherens junctions, and desmosomes [14] . (plos.org)
  • This line represents a human in vitro model to study the genetic basis of DCM. (bvsalud.org)
  • Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
  • This cell line can be successfully differentiated into cardiomyocytes and endothelial cells. (bvsalud.org)
  • Transfected cells were stained with HLA-E and/or HLA-G antibody. (iupui.edu)
  • Immunocytochemistry/ Immunofluorescence: MORF4L2 Antibody [NBP2-47370] - Staining of human cell line A-431 shows localization to nucleoplasm. (novusbio.com)
  • Western Blot: MORF4L2 Antibody [NBP2-47370] -Analysis in human cell line MCF-7. (novusbio.com)
  • Immunohistochemistry-Paraffin: MORF4L2 Antibody [NBP2-47370] - Staining of human cerebral cortex shows strong nuclear positivity in neurons. (novusbio.com)
  • Detection of TLR2 in transfected cell lysate using this antibody. (novusbio.com)
  • This antibody was developed against a mixture of synthetic peptides containing amino acids 180-196, 353-370, and 473-489 of human TLR2 (NP_003255). (novusbio.com)
  • Using a subviral particle vaccine candidate, we show here that one protein complex of HCMV, termed the pentameric complex (PC), enhances the neutralizing antibody response against viral infection of different cell types. (asm.org)
  • The HBMECs were treated with oxygen and glucose deprivation (OGD) to establish the ischemic cell model. (hindawi.com)
  • RESEARCH DESIGN AND METHODS- Human microvascular HMEC-1 endothelial cells were incubated in low and high glucose concentrations (5 and 30 mmol/l, respectively), and activation of nrf2 was assessed by nuclear translocation. (diabetesjournals.org)
  • Our understanding of the role of glucose transport in the lung and the mechanisms that regulate glucose movement across the human lung epithelium lags far behind that of the gut and kidney. (ersjournals.com)
  • In immortalised human airway cells (line H441) with 10 mM glucose in the basolateral medium, apical ASL glucose was 0.24±0.07 mM [ 10 ]. (ersjournals.com)
  • Current model of the mechanisms controlling glucose concentrations in the surface liquid lining the airway and distal lung epithelium. (ersjournals.com)
  • Background: Regarding recent findings on the proven influence of laser light on healing and endothelial regeneration processes, this study was conducted in order to examine the influence of low-power laser illumination on the endothelial inflammatory response. (iiarjournals.org)
  • The complement system is a group of proteins that work together to destroy foreign invaders (such as bacteria and viruses), trigger an inflammatory response, and remove debris from cells and tissues. (medlineplus.gov)
  • Our results provide insights into the molecular mode of action by which TR-lincRNAs contribute to complex human traits. (nih.gov)
  • A coordinated network of molecular processes providing cells with nearly flawless surveillance, maintenance, and repair capabilities exemplifies the "perfection" of the human body. (the-scientist.com)
  • Yet, if the molecular, cellular, and genetic machinery used to conceive, develop, and operate a human were designed rather than the result of evolution, humans would be different and life would look different. (the-scientist.com)
  • Methods: This study aims to demonstrate the use of HLA class I molecules in inducing human NK cell tolerance. (iupui.edu)
  • Normally, labs and companies perform liver-safety tests in lab rats and in single layers of liver cells called hepatocytes, grown in Petri dishes. (popsci.com)
  • Genetically modified pECs were co-cultured with human peripheral blood mononuclear cells (PBMCs) for E:T ratio of 10:1 for 2 hours. (iupui.edu)
  • Studies have shown that periodontitis is associated with altered expression of inflammatory mediators in gingival fibroblasts and periodontal membrane cells ( 3 , 4 ). (spandidos-publications.com)
  • Natural killer (NK) cells, with inhibitory and activating receptors, play unique roles in AXR by promoting graft rejection or tolerance. (iupui.edu)
  • Cells will adapt by activating the expression of genes involved in metabolic adaptation, such as those involved in glycolysis. (bmj.com)
  • It has shown that miR-429 could inhibit the development of colorectal cancer via targeting in large tumor suppressor kinase (LATS2), and decreased miR-429 could promote the expression of SRY-box transcription factor 2 (SOX2) and B-cell lymphoma 2 (BCL2) to attenuate the neuronal injury induced by accumulation of amyloid β -protein [ 12 ]. (hindawi.com)
  • The ability to support living human tissues with vascular channels is a huge step toward the goal of creating functional human organs outside of the body," says Wyss Institute Founding Director Donald Ingber. (cosmosmagazine.com)
  • The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
  • TMAO treatment for either 24H or 48H induces reduced cell viability and enhanced oxidative stress. (nature.com)
  • Low ATP concentrations in hypoxic tumour cells disable the apoptotic cascade and induce cell death by necrosis. (bmj.com)
  • Les concentrations sériques de sCD40L circulant et d'interleukine 10 circulante ont été analysées à l'aide de la méthode immuno-enzymatique chez 30 patients positifs pour le VHC avec un CHC, chez 30 patients patients positifs pour le VHC avec une cirrhose du foie, et chez 30 volontaires d'âge correspondant en bonne santé avec des anticorps anti-VHC négatifs servant de groupe témoin. (who.int)
  • Dr. Pienta received his B.A. in human biology from the Johns Hopkins University. (hopkinsmedicine.org)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • We asked experts in gerontology, neuroscience, genetics, cell biology, development, and health and fitness science to devise a human that would stand the test of time. (the-scientist.com)
  • In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
  • The obtained EURACi015-A line, showed the typical morphology of pluripotent cells, normal karyotype and exhibited pluripotency markers and a trilineage differentiation potential. (bvsalud.org)
  • Dynamic changes in airway epithelial paracellular permeability allow for receptor-ligand access across the airway epithelium, and thereby regulate cell signaling [11] . (plos.org)
  • NK cell tolerance occurs naturally in utero where human leukocyte antigen (HLA)-E and HLA-G are present. (iupui.edu)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • But the fragility of endothelial cells during laboratory growth has limited the utilization of this critical cell type, which is found in all vasculature. (sciencedaily.com)
  • Consideration of the genetic identity of both the Dengue and Zika flaviviruses as well as the cross-reactivity of relevant T cells along with the actions of CD4+ cytotoxic cells in these infections are also presented. (frontiersin.org)
  • Expressing HLA-E/G in xenografts may provide immune protection from human NK cell cytotoxicity. (iupui.edu)
  • Endothelial cells (ECs) form a semi-permeable monolayer that separates the bloodstream from underlying tissues and regulate the infiltration of blood cells and proteins through the vessel wall ( 1 , 2 ). (spandidos-publications.com)
  • Ultimately, we'd like to 3-D print with living cells, a process known as 3-D bioprinting, to create fully vascularized tissues for therapeutic applications," said Miller, assistant professor of bioengineering. (sciencedaily.com)
  • For the analysis of GSLs, they are extracted from tissues and cells using organic solvents. (springer.com)
  • 3-D printing helps researchers build living tissues one cell at a time. (popsci.com)
  • Apoptosis refers to the self-regulated and orderly death of cells controlled by genes in order to maintain the stability of internal environment ( 8 ). (spandidos-publications.com)
  • The apoptosis of endothelial cells is a key event that may impair the integrity of the vessel wall and lead to the formation of atherosclerotic plaques ( 9 ). (spandidos-publications.com)
  • A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)
  • UCN-01, a protein kinase C (PKC) inhibitor, induces growth arrest and apoptosis in cancer cells and was recently introduced in a phase I clinical trial. (karger.com)
  • A hypoxic environment devoid of nutrients prevents the cell undergoing energy dependent apoptosis and cells become necrotic. (bmj.com)
  • In HERA, rescue from degradation depends on FcRn, and engineered ligands are recycled in a manner that correlates with their half-lives in human FcRn transgenic mice. (nature.com)
  • Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)