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  • methods
  • PARTICIPANT/MATERIALS, SETTING, METHODS: We cultured human preimplantation embryos overnight in a time-lapse imaging system, in control or in a nocodazole-containing medium that prevents the formation of a proper mitotic spindle. (vub.be)
  • cells
  • The FISH data suggest that in compacted Day-4 embryos, approximately one in six cells started a polyploid new cell cycle rather than to go in apoptosis after the failure to maintain the SAC-mediated M-phase arrest. (vub.be)
  • These results suggest that during early embryo development, blastomeres with unresolved chromosome misalignments during M-phase can escape SAC-mediated apoptosis, continue cell division which can then result in aneuploid daughter cells. (vub.be)
  • mitotic
  • The embryos were subsequently fixed and analysed by immunocytochemistry for tubulin or mitotic and apoptotic markers, or by FISH. (vub.be)
  • Cas9
  • directly injected Cas9 protein into human embryos to correct a dominant mutation in MYBPC3 , which causes severe heart defects, and evaluated the rates of both gene correction and off-target editing. (sciencemag.org)
  • Interestingly, the authors showed that in heterozygous embryos, formed from a normal oocyte fertilized by sperm carrying a MYBPC3 mutation, Cas9-mediated editing is not only efficient but preferentially uses the normal maternal gene rather than the coinjected DNA for template-directed repair. (sciencemag.org)
  • Further, the authors noted that mosaicism, in which not all cells in the embryos were equally edited, occurred when Cas9 protein was delivered after multiple copies of the genome had been formed. (sciencemag.org)
  • However, early coinjection of Cas9 along with sperm, when only one copy of mutated DNA is present in the fertilized oocyte, eliminated mosaic embryos. (sciencemag.org)
  • Scientist
  • Even with ESCs from fertilised embryos, it takes a year to get a fully characterised stem cell line," he told New Scientist . (newscientist.com)
  • A top scientist in animal cloning, Chen Dayuan, praised the experiment, saying the generation of human blastulas would hopefully heal patients by replacing cells and organs under pathological change with ones their bodies had developed by healthy means. (trend.az)
  • Scientist He Jiankui attends the International Summit on Human Genome Editing at the University of Hong Kong in Hong Kong, China, Nov. 28. (catholicweekly.com.au)
  • contend
  • Biotechnology companies and patient advocates say they agree that no patents should be issued on human embryos, but they contend that the House language is overly broad. (latimes.com)
  • diseases
  • The ultimate aim of the research will be to harness knowledge gained from understanding the development of embryos, to develop treatments for serious diseases like diabetes. (newscientist.com)
  • SCNT has been used to clone animals before, and is thought to have potential applications in the study and treatment of human diseases. (www.nhs.uk)
  • experiments
  • Three separate studies were published in scientific journals describing Chinese experiments on gene editing in human embryos. (cnn.com)
  • The goal of his experiments was to make changes to the human embryo that could be passed on to future generations. (theconversation.com)
  • There's a lot of gene editing that goes on in agriculture and in animals and there have bene some experiments and attempts that have gone on in humans, very carefully done, that have gone on since the 1990s," he added. (catholicweekly.com.au)
  • vitro
  • I am fully supportive of research being carried out on early human embryos in vitro , especially on embryos that are not required for reproduction and would otherwise be discarded. (bionews.org.uk)
  • The 14-day limit on research in vitro would apply and it would be illegal in the UK to implant any such manipulated embryo into a woman for further development. (bionews.org.uk)
  • work
  • These problems lead the authors to suggest that a lot more work will be required to alter the techniques for use in human embryos. (bionews.org.uk)
  • Those concerns drove leading researchers to issue urgent calls in major scientific journals last month to halt such work on human embryos, at least until it could be proved safe and until society decided if it was ethical. (nytimes.com)
  • Noting the many unresolved questions about gene editing of human embryos, a group of leading American researchers recently published a paper in the journal Science calling for a moratorium on doing such work for clinical purposes. (nytimes.com)
  • early
  • As expected, the results confirm that OCT4 plays a key role in early human development, just as it does in mice. (newscientist.com)
  • A better understanding of early human development could help us develop better formulas. (newscientist.com)
  • A blastula is an embryo in the early stage of development. (trend.az)
  • concerns
  • Harvard University President Lawrence Summers said the potential benefits of the program outweighed the concerns of those who felt it challenged the sanctity of human life. (hindustantimes.com)
  • I'm sure it isn't done every day of the week due to the ethical concerns, but I couldn't see how cloning embryos would present any difficulty at all. (slashdot.org)
  • critics
  • Anti-abortion groups and other critics say the industry aims to kill the amendment because, in fact, it wants the right to patent certain embryos. (latimes.com)
  • When researchers announced last year that they had edited human embryos to repair a damaged gene that can lead to heart failure, critics called the report into question. (sciencenews.org)
  • Dolly
  • The embryo-creation technique is essentially the same as that used to create Dolly the sheep and the many cloned animals that have followed. (kottke.org)
  • stages
  • The objectives of this monograph are to provide a reasonably detailed morphological account of the human embryo (i.e., the first eight weeks of development), a formal classification into developmental stages, a catalogue of the preparations in the Carnegie Collection, and a reference guide to important specimens in other laboratories. (ehd.org)
  • embryos should be arranged in stages. (ehd.org)
  • cell
  • This involves removing the nucleus of a human egg cell and replacing it with the nucleus from a body cell. (newscientist.com)
  • The method has already been used in a wide variety of animal models and in human cell lines. (bionews.org.uk)
  • Unlike
  • The latter, however, unlike the Nomina anatomica, is not exclusively human, and hence certain inappropriate terms have been replaced here. (ehd.org)