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Embryonic Stem CellsCell DifferentiationStem CellsPluripotent Stem CellsCell Culture TechniquesEmbryo, MammalianOctamer Transcription Factor-3Embryo ResearchCell LineageStem Cell TransplantationCell LineInduced Pluripotent Stem CellsTeratomaCells, CulturedGene Expression Regulation, DevelopmentalEmbryoid BodiesStage-Specific Embryonic AntigensHematopoietic Stem CellsEndodermHomeodomain ProteinsSOXB1 Transcription FactorsCell ProliferationNeural Stem CellsFeeder CellsGerm LayersTotipotent Stem CellsGene Expression ProfilingBone Morphogenetic Protein 4Reverse Transcriptase Polymerase Chain ReactionTranscription FactorsSignal TransductionAdult Stem CellsKaryotypingCoculture TechniquesGene Expression RegulationActivinsFlow CytometryBlastocystBiological MarkersMesenchymal Stromal CellsStem Cell ResearchMultipotent Stem CellsNeuronsMice, SCIDImmunohistochemistryGreen Fluorescent ProteinsCell SeparationRegenerative MedicineRNA, MessengerMyocytes, CardiacCell SurvivalNeural PlateNuclear ReprogrammingGene ExpressionTretinoinEmbryonic DevelopmentMesodermEpigenesis, GeneticGene TargetingPhenotypeNodal ProteinFibroblastsHematopoiesisMice, Inbred C57BLGerm CellsDNA-Binding ProteinsCell DivisionCulture Media, Serum-FreeBone Morphogenetic ProteinsModels, BiologicalBase SequenceEmbryo Culture TechniquesOligonucleotide Array Sequence AnalysisStem Cell NicheEmbryonal Carcinoma Stem CellsTime FactorsLentivirusMolecular Sequence DataCulture Media, ConditionedTissue EngineeringMesenchymal Stem Cell TransplantationHematopoietic Stem Cell TransplantationSOXF Transcription FactorsCell ShapeTransgenesChimeraCloning, OrganismCarcinoma, EmbryonalPromoter Regions, GeneticTranscription, GeneticAntigens, DifferentiationCulture MediaLeukemia Inhibitory FactorMice, TransgenicEctodermMicroRNAsCell DedifferentiationX Chromosome InactivationGene Knockdown TechniquesCell Transplantation
VitroHESCsPluripotencyCapacity to differentiateRegenerativeMesenchymal stemProgenitorHESC differentiationLineagesHematopoieticAnalysis of human embryonicCardiomyocytesFibroblastsGerm layersExtra-embryonicTissuesTissueLineageAdultEmbryoESCsTransplantationGenesFunctionalSelf-renewalOptimized differentiation protocolVivoInduceRegulateTherapeuticPluripotent stem cells deProliferation and differentiationAbstractAssayPrecursorsTerminally differentiatedSomaticNeurons and gliaProtocolsEmbryosCardiomyocyteHepatocytesGenomePromotesNeuronalProgenitorsUpon differentiationFACSCharacterizationMethodsProteinsCellularExperimentalReproducibleResearch on embryonMaturationGene expressionTherapyPopulationsProliferativeOccursBiologyOsteoblastsTypes
Vitro20
  • Little is known about early trophoblast differentiation in the human embryo, owing to lack of a proper in vitro model system. (ca.gov)
  • Accelerated uptake of ESC based cell therapy on in vivo and in vitro principle is expected to fuel growth through to 2024. (grandviewresearch.com)
  • When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
  • This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
  • The comprehensive miRNA profiling presented here reveals novel sets of miRNAs differentially expressed during human neural cell fate determination in vitro. (muni.cz)
  • Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
  • The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
  • These old cells were reprogrammed in vitro to induced pluripotent stem cells (iPSC) and to rejuvenated and human embryonic stem cells (hESC): cells of all types can again be differentiated after this genuine "rejuvenation" therapy. (eurekalert.org)
  • They then completed the in vitro reprogramming of the cells. (eurekalert.org)
  • These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. (rndsystems.com)
  • These ESCs have the ability to remain undifferentiated and proliferate indefinitely in vitro , while maintaining the potential to differentiate into all three embryonic germ layers [1,2]. (bioinbrief.com)
  • The in vitro differentiation of ESCs into cardiomyocytes provides an opportunity to study the developmental aspects of cardiomyogenesis. (bioinbrief.com)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • Among them is the first international research on human pluripotent stem cells in vitro hematopoietic differentiation of human embryonic stem cells under space conditions. (pdnews.cn)
  • Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
  • With the formation of neural rosettes, neural tube development and neural differentiation can be modeled using human pluripotent stem cells (hPSCs) in vitro . (creative-biolabs.com)
  • The hPSCs-based neural rosettes have been widely used as models for the research of neural tube development as well as neural differentiation in vitro . (creative-biolabs.com)
  • Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation. (creative-biolabs.com)
  • The discovery of murine norovirus (MNV) in 2003 and the fact that this virus successfully replicated in a murine macrophage cell line in vitro and in primary immune cells in vivo suggested that immune cells may also support replication of human norovirus ( 7 ). (cdc.gov)
  • In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
HESCs26
  • Here, we show that BMP4-treated hESCs differentiate into bona fide CTB by direct comparison with primary human placental tissues and isolated CTB through gene expression profiling. (ca.gov)
  • Finally, we illustrate that downregulation of p63 by shRNA inhibits differentiation of hESCs into functional trophoblast. (ca.gov)
  • Taken together, our results establish that BMP4-treated hESCs are an excellent model of human trophoblast differentiation, closely mimicking the in vivo progression from p63+ CTB stem cells to terminally differentiated trophoblast subtypes. (ca.gov)
  • To bridge this gap, we used three model cell types to study miRNA expression patterns: human embryonic stem cells (hESCs), hESCs-derived self-renewing neural stem cells (NSCs), and differentiating NSCs. (muni.cz)
  • Our data also indicates that miRNA clusters enriched in NSCs share the target-determining seed sequence with cell cycle regulatory miRNAs expressed in pluripotent hESCs. (muni.cz)
  • Cell replacement using human embryonic stem cells (hESCs) derivatives may offer new therapeutic opportunities to replace degenerated neurons and repair damaged circuits. (lu.se)
  • Human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) are promising candidate cell sources for regenerative medicine. (jci.org)
  • However, despite the common ability of hiPSCs and hESCs to differentiate into all 3 germ layers, their functional equivalence at the single cell level remains to be demonstrated. (jci.org)
  • Here, we used single cell analysis to resolve the gene expression profiles of 362 hiPSCs and hESCs for an array of 42 genes that characterize the pluripotent and differentiated states. (jci.org)
  • hiPSCs also displayed slower growth kinetics and impaired directed differentiation as compared with hESCs. (jci.org)
  • Our results suggest that caution should be exercised before assuming that hiPSCs occupy a pluripotent state equivalent to that of hESCs, particularly when producing differentiated cells for regenerative medicine aims. (jci.org)
  • Human embryonic stem cells (hESCs) retain the extraordinary capacity to differentiate into different cell types of an adult organism, including pancreatic β-cells. (ox.ac.uk)
  • Our results indicate that treatment of hESCs (HS-181) with activin-A induced definitive endoderm differentiation as detected by the expression of SOX17 and FOXA2. (ox.ac.uk)
  • In conclusion, our strategy allows the efficient differentiation of hESCs into pancreatic endoderm capable of generating β-cell-like cells and demonstrates that RSV improves the maturation process. (ox.ac.uk)
  • As a newly discovered acylation modification, crotonylation of non-histone proteins remains largely unexplored, particularly in human embryonic stem cells (hESCs). (bioinfor.com)
  • Finally, we used knocked-down hESCs by shRNA, wild GAPDH and GAPDH mutants to explore potential role of GAPDH crotonylation in regulating human embryonic stem cell differentiation and metabolic switch. (bioinfor.com)
  • We found that induced crotonylation in hESCs resulted in hESCs of different pluripotency states differentiating into the endodermal lineage. (bioinfor.com)
  • We further discovered GAPDH as a key glycolytic enzyme regulated by crotonylation during endodermal differentiation from hESCs. (bioinfor.com)
  • Crotonylation of GAPDH decreased its enzymatic activity thereby leading to reduced glycolysis during endodermal differentiation from hESCs. (bioinfor.com)
  • Our data set up that hemogenic endothelium populations endowed with primitive and definitive hematopoietic potential are specified simultaneously from your mesoderm in differentiating hESCs. (bioskinrevive.com)
  • derivation of this specialized endothelium Ezetimibe enzyme inhibitor from human being embryonic stem cells (hESCs) provides an priceless platform to study and dissect blood specification and the emergence of hematopoietic stem and progenitor cells. (bioskinrevive.com)
  • In the last decade, there has been an increased desire for the characterization of this precursor from differentiating hESCs using several methods, generally through three-dimensional embryoid body (EB) differentiation (Ditadi et?al. (bioskinrevive.com)
  • 2014). Using OP9 stromal cells to differentiate hESCs, both Rafii et?al. (bioskinrevive.com)
  • We studied human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) measuring the SNPs and CNVs with Affymetrix SNP 6 microarrays and expression values with Affymetrix Exon microarrays. (biomedcentral.com)
  • Here, we engrafted hESCs differentiated toward a ventral midbrain DA phenotype into the midbrain of a preclinical rodent model of Parkinson's disease. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
Pluripotency25
  • The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. (nih.gov)
  • CHD1 is essential for embryonic stem cell pluripotency in mice by maintaining an open euchromatic chromatin state. (wikipedia.org)
  • And while in human pluripotent stem cells, miRNAs have been directly linked to the core pluripotency network, including the cell cycle regulation and the maintenance of the self-renewing capacity, their role in the onset of differentiation in other contexts, such as determination of neural cell fate, remains poorly described. (muni.cz)
  • Discoveries in fundamental basic research have revealed how human embryonic stem cells regulate gene expression to maintain pluripotency, how induced pluripotent stem cells retain a "memory" of their origin, and how adult stem cells differentiate in response to their environmental milieu. (ca.gov)
  • Pluripotency means that the cells can differentiate into any cell type of the human body. (bayer.com)
  • To account for this high level of functional variation, verifying the pluripotency of stem cells-from the generation of a new cell line through its expansion for an experiment-is crucial. (genengnews.com)
  • To address these difficulties, R&D Systems has developed new tools that aid in the verification of stem cell pluripotency during derivation, maintenance, and expansion. (genengnews.com)
  • Moreover, colony morphology is not truly indicative of pluripotency, since cells that are not fully reprogrammed can have similar morphology. (genengnews.com)
  • The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
  • Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
  • Despite its broad use as the most common assay for assessing the pluripotency of stem cells, the teratoma assay has limitations. (genengnews.com)
  • Studies showed that any given population of ESCs could be very heterogeneous and that their potential pluripotency, or capacity to differentiate into other cell types, could vary both among cells from the same embryo and from one lineage to another. (eurekalert.org)
  • Researchers at the Center for Cell-Based Therapy (CTC) in Ribeirão Preto, São Paulo State, have now investigated the functioning of 31 miRNAs observed in human ESCs and identified signaling pathways involved in both pluripotency and differentiation. (eurekalert.org)
  • According to Panepucci, the human ESCs used in scientific research are usually in the intermediate development stage of primed pluripotency, when they have not yet differentiated but are primed, i.e., a step closer to assuming a distinct cell identity. (eurekalert.org)
  • After culture for three to four?days, automated image acquisition and analysis were performed to evaluate the effects on pluripotency maintenance and differentiation. (eurekalert.org)
  • Based on our analysis of this material, we established multiparameter phenotype profiles to determine the pluripotency stage of the cells and the effects of the miRNAs. (eurekalert.org)
  • Extremely, nevertheless, our outcomes confirmed that the bioreactor differentiated ESCs maintained their capability to CL 316243 disodium salt manufacture exhibit pluripotency indicators, to type ESC-like colonies, and to generate teratomas upon transplantation, whereas the cells differentiated in adherent lifestyle dropped these features. (bioinbrief.com)
  • The advancement of effective ESC difference protocols within suspension system bioreactors needs a even more comprehensive understanding of the affects of shear factors on the control of pluripotency and difference in pluripotent control cells. (bioinbrief.com)
  • Nevertheless, since we have previously observed that the suspension bioreactor environment enhances ESC pluripotency, while suppressing differentiation effectiveness [17], we wanted to investigate whether this trend would also happen in during cardiomyocyte differentiation. (bioinbrief.com)
  • This study demonstrates that suspension bioreactor tradition systems do indeed possess the ability to prevent differentiation, and actually induce 'transient' pluripotency within a defined differentiation protocol, presumably due to influence of shear stress on the cells. (bioinbrief.com)
  • Our data shows that in contrast to static tradition, ESC ethnicities caused to differentiate toward cardiomyocytes in suspension bioreactors retain some capability to exhibit pluripotency indicators, to type. (bioinbrief.com)
  • Promotes self-renewal and maintains pluripotency of human embryonic stem cells and mouse Epi-stem cells when used in combination with CHIR99021 (Kim et al. (stemcell.com)
  • Background: Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties: self renewal and pluripotency. (lu.se)
  • Also, under/over-expression studies of the master regulator Oct4 have revealed that some self-renewal/pluripotency as well as differentiation genes are expressed in a biphasic manner with respect to the concentration of Oct4. (lu.se)
  • TFs being expressed and the downstream differentiation target been established as candidates to regulate both pluripotency and genes being shut off [11]. (lu.se)
Capacity to differentiate2
  • Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
  • Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)
Regenerative21
Mesenchymal stem4
  • The ERK-MAPK signaling pathway plays a role in the differentiation of mesenchymal stem cells (MSCs) into adipocytes [1] [2] . (cusabio.com)
  • p38 pathway plays a very important role in the osteogenic differentiation of mesenchymal stem cells (MSCs). (cusabio.com)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)
Progenitor10
  • Small hepatic progenitor cells (SHPCs) derived from these hepatocytes have shown mature metabolic functions as well as engraftment in immunodeficient mice. (warf.org)
  • 1) Hepatocytes generated in this system display late fetal markers (both albumin and alpha-fetoprotein, AFP) and give rise to mature and engraftable small hepatic progenitor cells (SHPCs, which express albumin but not AFP). (warf.org)
  • Conclusions: The data support the long-term therapeutic potential of ESC-derived human striatal progenitor grafts for the replacement of degenerated striatal neurons in HD and suggest that EE can effectively accelerate the maturation and promote the integration of human striatal cells. (lu.se)
  • Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)
  • However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
  • Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
  • For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
  • Addition of retinoic acid (RA), Noggin and Cyclopamine promoted pancreatic differentiation as indicated by the expression of the early pancreatic progenitor markers ISL1, NGN3 and PDX1. (ox.ac.uk)
  • It is the developmental characteristic of neural progenitor cells in differentiated embryonic stem cell cultures. (creative-biolabs.com)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
HESC differentiation5
  • We also establish that, similar to in vivo events, hESC differentiation into trophoblast is characterized by a p63+/KRT7+ CTB stem cell state, followed by formation of functional KLF4+ STB and HLA-G+ EVT. (ca.gov)
  • Extensive reorganization of higher-order chromatin structure occurs during hESC differentiation. (nih.gov)
  • We investigated the role of crotonylation in hESC differentiation by introduce crotonate into the culture medium of GFP tagged LTR7 primed H9 cell and extended pluripotent stem cell lines. (bioinfor.com)
  • To time, it really is still as yet not known whether HE subsets with different hematopoietic potentials emerge in successive waves during hESC differentiation, whether HE populations are preserved inside the differentiating lifestyle as time passes, or whether one exclusive population of He's produced early from mesoderm and steadily differentiates inside the lifestyle. (bioskinrevive.com)
  • Following hemogenic potential of endothelium cell populations frequently during the period of hESC differentiation would address a few of these problems but to time this has hardly ever been reported. (bioskinrevive.com)
Lineages7
  • We describe high resolution, genome-wide dynamic chromatin interaction maps in human embryonic stem cells (hESC) as they differentiate into four distinct embryonic cell lineages. (nih.gov)
  • In addition, they do not require feeder layers and can differentiate into many neural lineages under the appropriate conditions (1). (ddw-online.com)
  • What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
  • These stem cells are considered committed to certain cell lineages. (biomedcentral.com)
  • Such human fully integrated and complete SEMs recapitulate the organization of nearly all known lineages and compartments of post-implantation human embryos, including the epiblast, the hypoblast, the extra-embryonic mesoderm and the trophoblast layer surrounding the latter compartments. (bvsalud.org)
  • Background Embryonic stem cells (ESCs) can proliferate endlessly and are capable to differentiate into every cell lineages that make up the mature organism. (bioinbrief.com)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Hematopoietic12
  • The inventors have developed a novel system for co-differentiation of hepatocytes and hematopoietic progenitors from human embryonic stem cells (ES cells) that mimics a late fetal liver undergoing hematopoiesis. (warf.org)
  • The differentiated hematopoietic precursors express mature markers that show promise for engraftment (study in progress). (warf.org)
  • For example, hematopoietic stem cells differentiate into various immune cells. (cusabio.com)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • Hematopoietic (blood forming) stem cells have been investigated for several decades and is currently by far the best characterized stem cell system. (biomedcentral.com)
  • Until recently it was considered a fact that, for example, hematopoietic stem cells did not have the potential to differentiate into cells of other types than the cells constituting the blood system. (biomedcentral.com)
  • In an interview with CCTV News, Lei Xiaohua, a researcher at the Institute of Biomedicine and Technology of Chinese Academy of Sciences, stated that the taikonauts aboard the Shenzhou-15 have successfully differentiated a hematopoietic stem cell similar to a cobblestone in orbit. (pdnews.cn)
  • This stem cell will further mature and differentiate to form a group of hematopoietic stem cells resembling a cluster of grapes. (pdnews.cn)
  • From the experimental results, China has successfully achieved the first hematopoietic differentiation of human embryonic stem cells under space conditions, accomplishing its first experimental goal. (pdnews.cn)
  • Lei said that in the next phase, the research team needs to conduct comprehensive detection and analysis of this group of hematopoietic stem cells, and screen out the relevant genes affecting the early hematopoiesis differentiation of human pluripotent stem cells in the space environment by comparing with the ground control group. (pdnews.cn)
  • 2013). The performance of hematopoietic differentiation differs between your two methodologies because of parameters such as for example serum, stromal maintenance, or EB size, amongst others elements (Kardel and Eaves, 2012, Vodyanik et?al. (bioskinrevive.com)
  • 2006). Using EB differentiation protocols, the onset of hematopoietic commitment was also defined from the manifestation of CD43, growing from a CD34+ endothelial precursor human population (Kennedy et?al. (bioskinrevive.com)
Analysis of human embryonic1
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Cardiomyocytes5
  • Cardiomyocytes are terminally differentiated muscle mass cells in the adult mammalian heart, which do not divide. (bioinbrief.com)
  • Such cell transplantation therapies would need the effective seeding of as many as 1 108 donor cardiomyocytes per individual [4]. (bioinbrief.com)
  • Presently, most protocols make use of stationary lifestyle to differentiate ESCs into cardiomyocytes [5-12]. (bioinbrief.com)
  • Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al. (stemcell.com)
  • the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)
Fibroblasts3
  • To define better cellular events that are specifically vulnerable to WRN deficiency, I used RNA interference (RNAi) to silence the expression of Wrn gene and other RecQ helicases, linked to different clinical phenotypes, in primary human fibroblasts. (yale.edu)
  • Researchers first multiplied skin cells (fibroblasts) from a 74 year-old donor to obtain the senescence characterized by the end of cellular proliferation. (eurekalert.org)
  • Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. (rndsystems.com)
Germ layers4
Extra-embryonic5
  • Its main functional component is the trophoblast, which is derived from extra-embryonic ectoderm. (ca.gov)
  • However, they cannot form an organism because they are unable to give rise to extra embryonic tissue essential for normal development in the uterus. (biomedcentral.com)
  • Embryo-like models with spatially organized morphogenesis and structure of all defining embryonic and extra-embryonic tissues of the post-implantation human conceptus (that is, the embryonic disc, the bilaminar disc, the yolk sac, the chorionic sac and the surrounding trophoblast layer) remain lacking1,2. (bvsalud.org)
  • Mouse naive embryonic stem cells have recently been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured stem-cell-based embryo models with spatially organized morphogenesis (called SEMs)3. (bvsalud.org)
  • These include embryonic disc and bilaminar disc formation, epiblast lumenogenesis, polarized amniogenesis, anterior-posterior symmetry breaking, primordial germ-cell specification, polarized yolk sac with visceral and parietal endoderm formation, extra-embryonic mesoderm expansion that defines a chorionic cavity and a connecting stalk, and a trophoblast-surrounding compartment demonstrating syncytium and lacunae formation. (bvsalud.org)
Tissues15
  • Stem cells derived through such techniques can be converted into liver, nerve, and heart tissues. (grandviewresearch.com)
  • These cells then form specific tissues and organs in the human body. (labroots.com)
  • 27 Jun, 2007 06:08 pm Stem cells have the potential to become all the cells and tissues in the human body. (scitizen.com)
  • Embryonic stem cells (ESCs) can give rise to many different types of tissues and organs. (eurekalert.org)
  • This research paves the way for the therapeutic use of iPS, insofar as an ideal source of adult cells is provided, which are tolerated by the immune system and can repair organs or tissues in elderly patients. (eurekalert.org)
  • A few of the cells in the inner cell mass will develop into the foetus, whereas the rest will form the placenta and other supporting tissues needed for foetal development in the uterus. (biomedcentral.com)
  • Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
  • Adult stem cells are found in different tissues and organs and are more limited in their differentiation potential. (globalstemcelltherapy.com)
  • Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
  • In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)
  • The first steps toward a new cell culture system for human norovirus included the detection of human norovirus antigen in duodenal and jejunal enterocytes in tissue sections from human norovirus-infected transplant patients ( 11 ) and the development of human intestinal enteroids (HIEs) derived from nontransformed small intestine and colonic tissues ( 12 ). (cdc.gov)
  • As greater understanding of the growth factors involved in wound healing emerges, future patient care may include scarless wound healing and transplant of tissues engineered from stem cell progenitors. (medscape.com)
  • Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
  • According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)
  • The stem cells suits human needs, does not cause harm and can be obtained from both adult and fetal does not conflict with religious beliefs, it has tissues, umbilical cord and early embryos. (who.int)
Tissue24
  • Controlled differentiation that can be achieved in tissue committed cells through the co-culturing with basic fibroblast growth factor is expected to induce growth. (grandviewresearch.com)
  • Embryonic stem cells are intriguing because they can differentiate into any of the specialized tissue cells of the body. (latimes.com)
  • This may allow scientists to culture healthy tissue cells in the lab to replace damaged ones in the human body. (latimes.com)
  • 8 Jun, 2007 04:13 pm Stem cells provide the starting material for the development and repair of every organ and tissue in the body and they are present in all stages of life. (scitizen.com)
  • Their capacity to originate any type of tissue is greater at that stage," said Rodrigo Alexandre Panepucci ( https://bv.fapesp.br/en/pesquisador/57383/rodrigo-alexandre-panepucci/ ), a researcher at the Ribeirão Preto Blood Center and principal investigator of the study. (eurekalert.org)
  • Methods - Use seamstress to design anatomical fit for glove and booty, and tissue culture strategies to incorporate stem and cardiomyocyte cells into 3D matrices. (webmedcentral.com)
  • This differentiation capacity makes ESCs an attractive cell source for cell/tissue alternative therapies for the treatment of human degenerative diseases. (bioinbrief.com)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • In recent years, foreign scientists have also reported several times on research regarding stem cell growth and tissue regeneration carried out during space flight missions, such as blood stem cells for astronauts with anemia. (pdnews.cn)
  • Experts said that the unique space microgravity environment may be a new way to maintain undifferentiated proliferation of stem cells, enhance the efficiency of induced differentiation, and improve the level of three-dimensional tissue construction. (pdnews.cn)
  • Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
  • The expression and regulation of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) in neuroectodermal precursor cells is undocumented. (uea.ac.uk)
  • Several genome-wide studies have reported genomic variants that correlate with gene expression levels in various tissue and cell types. (biomedcentral.com)
  • In explant method, the dental tissue is placed on a plastic surface and the cells migrate out from the tissue fragment adhering to culture flasks or dishes ( Figure 1 ). (bvsalud.org)
  • Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
  • Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)
  • Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. (cdc.gov)
  • The HIEs, or "mini-guts," are generated from stem cells present in the intestinal crypts isolated from human intestinal tissue and cultured indefinitely as ex vivo, 3-dimensional (3D) cultures in growth-factor-enriched media ( 13 - 15 ). (cdc.gov)
  • After injury to tissue occurs, the cell membranes, damaged from the wound formation, release thromboxane A2 and prostaglandin 2-alpha, potent vasoconstrictors. (medscape.com)
  • Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
  • By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)
  • Furthermore, pathways controlling the self-renewal capacity of normal tissue SCs are frequently deregulated in human cancer. (medscape.com)
  • One of the greatest controversies triggered tissue, a stem cell encoding for heart tissue by the rapid pace of evolution in biology, will eventually develop into heart tissue particularly in genomics and biotechnology, and so on. (who.int)
Lineage9
  • The ability of human parthenogenetic (PG) embryonic stem cells (hpESCs) to undergo neural lineage and cell type-specific differentiation is undefined. (uni-wuerzburg.de)
  • Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)
  • Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
  • For this particular lineage, although a lot of effort has been made in the last ten years to achieve an efficient and reproducible differentiation protocol, it was not until recently that this aim was roughly accomplished. (ox.ac.uk)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
  • Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. (ku.dk)
  • Through morphological changes, qPCR of pluripotent and germ layer-specific gene markers and flow cytometry analysis, we determined that the induced crotonylation resulted in hESC differentiating into the endodermal lineage. (bioinfor.com)
  • In particular, an excess of Cdx2 over Oct4 determines the trophectoderm lineage whereas an excess of Gata-6 over Nanog determines differentiation into the endoderm lineage. (lu.se)
  • Citation: Chickarmane V, Peterson C (2008) A Computational Model for Understanding Stem Cell, Trophectoderm and Endoderm Lineage Determination. (lu.se)
Adult12
Embryo7
  • The effect of the DAZ family members on the embryonic stem cells varied according to whether the cells were derived from a male or a female embryo. (sciencedaily.com)
  • However, implementation remains a controversial topic due to diverse views in context to the moral and legal status of early embryo cell lines. (grandviewresearch.com)
  • A fertilized egg is totipotent, meaning that its potential is total, and as the fertilized egg divides all the cells in the embryo remain totipotent until the fertilized egg has reached the 8/16-cell stage. (biomedcentral.com)
  • In theory, and to some extent in practice, an 8-cell embryo can be divided into eight single cells, and each cell has the potential of generating an individual if implanted in a woman's uterus. (biomedcentral.com)
  • The totipotent embryo initiates transcription during zygotic or embryonic genome activation (EGA, ZGA). (bvsalud.org)
  • 3,4 The zygote and cells derived from the first two cellular divisions constitute the most primitive cells (totipotent cells) that are capable of forming the embryo and the embryonic annexes (e.g. placenta, amniotic membranes etc). (bvsalud.org)
  • PBK model allows the prediction of dose-response curves for implantation rat whole-embryo culture test, the rat limb bud human developmental toxicity. (cdc.gov)
ESCs10
  • These cells have been described as embryonic stem cells (ESCs) and. (scitizen.com)
  • At the turn of the present century, these cells were believed to offer hope of treatment for several health problems, but as research advanced, scientists realized that understanding and controlling the behavior of ESCs would be a more daunting challenge than initially imagined. (eurekalert.org)
  • At this stage, they are less versatile than mouse ESCs, which are typically isolated when the cells are naive and hence widely used as a research model. (eurekalert.org)
  • From hundreds of morphological parameters observed in the images, we selected approximately ten that enabled us to classify the differentiation stage of the ESCs," Panepucci said. (eurekalert.org)
  • History Embryonic control cells (ESCs) are made from the internal cell mass (ICM) of pre-implantation embryos [1]. (bioinbrief.com)
  • Moreover, ESCs can also be used as a model system for understanding human genetic disease by elucidating the pathophysiology of specific genetic disorders, including but not limited to cardiac abnormalities. (bioinbrief.com)
  • Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
  • Moreover, the combination of gene modification and directed differentiation of ESCs provides perfect tool for disease modelling and drug discovery. (benthamscience.com)
  • What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)
  • However, the derivation of human NT-ESCs goes with the destruction of clone embryos, leading to fierce ethical disputes. (benthamscience.com)
Transplantation10
  • This study describes for the first time, a differentiation system that does not require co-culture with animal cells or products, thus allowing the production of pathogen-free RPE cells suitable for transplantation into patients. (news-medical.net)
  • Results: Human striatal progenitors survived up to 6 months after transplantation and showed morphological and neurochemical features typical of human MSNs. (lu.se)
  • Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
  • in 1956 and Till and McCulloch in 1961, demonstrating that lethally irradiated mice could be rescued with cells from freshly isolated bone marrow [ 10 ] and that BM cells formed spleen clonies (CFU-S) following transplantation to irradiated recipients [ 30 ], respectively. (biomedcentral.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • Transplantation in Parkinson's disease using human embryonic stem cell (hESC)-derived dopaminergic (DA) neurons is a promising future treatment option. (lu.se)
  • This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
  • We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
  • The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
  • Treatment selection takes into account patient age, fitness, and whether autologous stem cell transplantation (ASCT) is planned. (medscape.com)
Genes13
  • Until now we've relied on studies in mice to understand human germ cell differentiation, but the reproductive genes are not the same. (sciencedaily.com)
  • In the current study, the researchers treated human embryonic stem cells with proteins known to stimulate germ cell formation and isolated those that began to express germ-cell-specific genes -- about 5 percent of the total. (sciencedaily.com)
  • In addition to expressing key genes, these cells also began to remove modifications, or methyl groups, to their DNA that confer cell-specific traits that would interfere with their ability to function as germ cells. (sciencedaily.com)
  • It is thought that human CHD1 preferentially binds this histone modification, which is primarily located at the 5' regions of genes, as a mechanism of recruitment to those genomic loci. (wikipedia.org)
  • Our results demonstrate that despite the lack of a paternal genome, hpESCs generate proliferating NSCs that are capable of differentiation into physiologically functional neuron-like cells and maintain allele-specific expression of imprinted genes. (uni-wuerzburg.de)
  • My research interest focuses on how genes and genetic mechanisms contribute to childhood developmental or late-onset disorders of the human nervous system. (yale.edu)
  • The inventors have previously shown that such SHPCs express high levels of mature hepatic genes, perform xenobiotic metabolism at rates equal to primary human hepatocytes, and support engraftment in immunodeficient mice (P180325). (warf.org)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • Our results revealed altogether 217 genes and 584 SNPs whose genomic alterations affect the transcriptome in the same cells. (biomedcentral.com)
  • Our results revealed that in the human pluripotent stem cells, the expression values of several genes, transcripts and exons were affected due to the genomic variation. (biomedcentral.com)
  • The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
Functional12
  • This is the first evidence that you can create functional human germ cells in a laboratory. (sciencedaily.com)
  • Using this knowledge, we have determined that human embryonic stem cells, when treated with the chemical BMP4, undergo this 'cytotrophoblast stem cell state' before becoming fully functional placental cells. (ca.gov)
  • Such analyses can reveal detailed expression dynamics and functional relationships for differentiation. (keio.ac.jp)
  • 10 Oct, 2007 12:18 pm The field of functional genomics explores the various functions of genetic sequences within the human genome. (scitizen.com)
  • Inserm's AVENIR "Genomic plasticity and aging" team, directed by Jean-Marc Lemaitre, Inserm researcher at the Functional Genomics Institute (Inserm/CNRS/Université de Montpellier 1 and 2), has recently succeeded in rejuvenating cells from elderly donors (aged over 100). (eurekalert.org)
  • Using this new "cocktail" of six factors, the senescent cells, programmed into functional iPSC cells, re-acquired the characteristics of embryonic pluripotent stem cells. (eurekalert.org)
  • Signs of aging were erased and the iPSCs obtained can produce functional cells, of any type, with an increased proliferation capacity and longevity," explains Jean-Marc Lemaitre who directs the Inserm AVENIR team. (eurekalert.org)
  • The aim of this study was to optimize an efficient differentiation protocol that mimics in vivo pancreatic organogenesis and to investigate whether RSV may improve the final maturation step to obtain functional insulin-secreting cells. (ox.ac.uk)
  • Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
  • After sequencing the human genome, numerous projects have focused on characterizing genomic alterations and associating them with the different diseases or functional elements of the genome. (biomedcentral.com)
  • Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
  • A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
Self-renewal3
  • The involvement of microRNAs (miRNAs) in orchestrating self-renewal and differentiation of stem cells has been revealed in a number of recent studies. (muni.cz)
  • In particular, they recovered their capacity for self-renewal and their former differentiation potential, and do not preserve any traces of previous aging. (eurekalert.org)
  • SCs are not only capable of maintaining themselves (self-renewal) but also give rise to a proliferative 'transit amplifying' compartment and ultimately to postmitotic differentiated cells with specialized functions. (medscape.com)
Optimized differentiation protocol1
  • Resveratrol ameliorates the maturation process of β-cell-like cells obtained from an optimized differentiation protocol of human embryonic stem cells. (ox.ac.uk)
Vivo2
  • This is the first application of transcriptomics to assess ES cell derivatives and their in vivo counterparts -- a 'differentiomics' outlook. (news-medical.net)
  • Advances in bioengineering have addressed problems of how to control growth and differentiation of stem cells using novel biomaterials, how to sort and purify specific stem cell products, and how to deliver and sustain stem cell grafts in vivo. (ca.gov)
Induce3
  • JNK can change the mRNA level of osteocalcin , so the activation of JNK can induce osteoblast differentiation. (cusabio.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Regulate1
Therapeutic7
  • Rapid advancements in this vertical include protocols for directed differentiation, defined culture systems, demonstration of applications in drug screening, the establishment of various disease models, and evaluation of therapeutic potential in treating incurable diseases. (grandviewresearch.com)
  • With therapeutic cloning, or the creation of banks of stem cells generated using parthenogenesis (perhaps as few as 40-400 lines), a limitless supply of immune-matched cells could be generated for most of the population. (news-medical.net)
  • The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
  • The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
  • This cell then has therapeutic cloning: the global the capacity to divide and grow into an exact replica of the original from whom the debate somatic cell was taken. (who.int)
Pluripotent stem cells de1
Proliferation and differentiation2
  • This pathway can also affect the proliferation and differentiation of red blood cells. (cusabio.com)
  • In 2017, the research team used the Tianzhou-1 cargo spacecraft to conduct research on the proliferation and differentiation of embryonic stem cells on a mouse. (pdnews.cn)
Abstract1
  • abstract = 'Mitochondrial dysfunction has been demonstrated to result in premature aging due to its effects on stem cells. (ku.dk)
Assay4
  • This results in differing cytotoxic responses that are influenced by the nature of target cells, assay types and concentrations of ATZ. (frontiersin.org)
  • These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)
  • The teratoma assay is also inherently variable, depending on the site of cell inoculation and the mouse strain used. (genengnews.com)
  • showed that the differentiation assay of benchmark dose. (cdc.gov)
Precursors3
  • Researchers at the school have devised a way to efficiently coax the cells to become human germ cells -- the precursors of egg and sperm cells -- in the laboratory. (sciencedaily.com)
  • We have previously shown that p63, a member of the p53 family of nuclear proteins, is expressed in proliferative cytotrophoblast (CTB), precursors to terminally differentiated syncytiotrophoblast (STB) in chorionic villi and extravillous trophoblast (EVT) at the implantation site. (ca.gov)
  • Hence, regulation of selective MMPs and TIMPs occurs during differentiation of human neural precursors suggesting that MMP-2 and TIMP-4 in particular may perform regulatory roles in the developing CNS. (uea.ac.uk)
Terminally differentiated1
  • An important aspect of ESC research focuses on elucidating the mechanisms of differentiation from the pluripotent ESC to numerous terminally differentiated cell types. (bioinbrief.com)
Somatic3
  • Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
  • Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). (rndsystems.com)
  • Here we characterized the persistence and emergence of these epigenetic differences by performing genome-wide DNA methylation profiling throughout primed and naive reprogramming of human somatic cells to hiPS cells. (bvsalud.org)
Neurons and glia2
Protocols10
  • Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
  • Finally, differentiation protocols incorporating RSV treatment yielded numerous insulin-positive cells, induced significantly higher PDX1 expression and were able to transiently normalize glycaemia when transplanted in streptozotocin (STZ) induced diabetic mice thus promoting its survival. (ox.ac.uk)
  • Basic Cell Culture Protocols 4th Ed. By Helgason, Cheryl D., Miller, Cindy L. (Eds. (dattanibookagency.in)
  • Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. (dattanibookagency.in)
  • Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success. (dattanibookagency.in)
  • To date, a great deal of data describing the emergence of blood cells from human being HE have been acquired using stromal co-culture protocols Ezetimibe enzyme inhibitor (Choi et?al. (bioskinrevive.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
Embryos3
  • Human embryonic stem cells derived from excess IVF embryos may help scientists unlock the mysteries of infertility for other couples struggling to conceive, according to new research. (sciencedaily.com)
  • For instance, Science University and Oregon Health are engaged in developing blastocyst staged embryos of around 150 cells in order to provide hESC source. (grandviewresearch.com)
  • Unicellular for those cells that are derived from human organisms are primed to replicate (clone) pre-embryos, which seem to have a high themselves by nature. (who.int)
Cardiomyocyte1
  • A conclusion This scholarly research demonstrates that although cardiomyocyte difference can CL 316243 disodium salt manufacture end up being attained in stirred suspension system bioreactors, the addition of moderate boosters is certainly not really sufficient to power comprehensive difference as liquid shear factors show up to maintain a subpopulation of cells in a transient pluripotent condition. (bioinbrief.com)
Hepatocytes5
Genome1
  • Parent of origin imprints on the genome have been implicated in the regulation of neural cell type differentiation. (uni-wuerzburg.de)
Promotes3
  • Studies in both yeast and humans have found that Chd1 is recruited to DNA damage sites, where it promotes the opening of chromatin and the recruitment of DNA repair factors, thus facilitating DNA repair by homologous recombination. (wikipedia.org)
  • This kit, used in combination with the analysis of colony morphology, promotes the selection of high-quality, undifferentiated stem cell colonies, thereby reducing wasted time. (genengnews.com)
  • In the early stage of adipocyte differentiation, ERK1/2 promotes adipocyte differentiation by promoting the expression of C/EBPα and PPARγ. (cusabio.com)
Neuronal2
  • Here we use cellular and animal models as well as human biospecimens to show that AD-related stressors mediate global disturbances in dynamic intra- and inter-neuronal networks through pathologic rewiring of the chaperome system into epichaperomes. (nature.com)
  • State-of-the art technologies are used to investigate global molecular changes that arise during neuronal fate specification and differentiation from pluripotent embryonic stem (ES) cells. (yale.edu)
Progenitors1
  • 2012). At later EB stage, most CD43+ cells upregulated the manifestation of CD41a and CD235a, and were enriched for megakaryocyte and erythroid progenitors, respectively (Klimchenko et?al. (bioskinrevive.com)
Upon differentiation2
  • Analysis of imprinting in hpESCs and in hpNSCs revealed that maternal-specific gene expression patterns and imprinting marks were generally maintained in PG cells upon differentiation. (uni-wuerzburg.de)
  • Interestingly, TIMP-4 also differed from the other TIMPs in that it was cell- associated in the stem cells, where this fraction remained unchanged upon differentiation. (uea.ac.uk)
FACS1
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
Characterization3
  • Mechanical manipulation and characterization of an individual biological cell is currently one of the most exciting research areas in the field of medical robotics. (umd.edu)
  • We hypothesize that force feedback plays an important role in cell manipulation and possibly helps in cell characterization. (umd.edu)
  • Thus, we hypothesize that mechanical characterization studies will potentially pave the way for developing a high throughput system with force feedback capability, to understand and predict the differentiation path a particular pluripotent cell will follow. (umd.edu)
Methods5
  • Advanced culturing methods can make it possible to direct the differentiation of the iPSCs to a certain type of cell, like specific brain cells or heart cells. (bayer.com)
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
Proteins6
  • Overexpressing the three proteins together allowed the researchers to generate haploid cells -- those with only one copy of each chromosome -- expressing proteins found in mature sperm. (sciencedaily.com)
  • This suggests that BOULE may play a larger role than the other proteins in the development of female germ cells. (sciencedaily.com)
  • Humans and mice, by contrast, have ten CHD proteins that are homologous to CHD1, but each have their own characteristic functions. (wikipedia.org)
  • One family of microdomains harbors the scaffolding flotillin protein FloA that selectively tethers proteins specialized in regulating cell envelope turnover and primary metabolism. (uni-wuerzburg.de)
  • Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
  • Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)
Cellular4
  • Atrazine (ATZ) is an environmental pollutant that interferes with several aspects of mammalian cellular processes including germ cell development, immunological, reproductive and neurological functions. (frontiersin.org)
  • Exploring the genetic, molecular and biological features of neural differentiation process from embryonic and patient-specific stem cells may offer an unprecedented chance to understand normal and abnormal human brain development and diseases, with implications for drug development and potential cellular replacement therapy. (yale.edu)
  • The researchers have successfully rejuvenated cells from elderly donors, some over 100 years old, thus demonstrating the reversibility of the cellular aging process. (eurekalert.org)
  • They have the physiological characteristics of "young" cells, both from the perspective of their proliferative capacity and their cellular metabolisms. (eurekalert.org)
Experimental6
  • ATZ also induces morphological changes similar to apoptosis and initiates mitochondria-dependent cell death in several experimental models. (frontiersin.org)
  • Unfortunately, properly characterizing these cells in order to maintain experimental consistency is proving to be a time-consuming and difficult task. (genengnews.com)
  • Our experimental results on zebrafish egg cells show that a system with force feedback capability when combined with vision feedback can lead to potentially higher success rates in cell injection tasks. (umd.edu)
  • This SEM platform will probably enable the experimental investigation of previously inaccessible windows of human early post implantation up to peri-gastrulation development. (bvsalud.org)
  • 2006). Moreover, in both these experimental strategies, the hemogenic potential of endothelium precursor people has been examined at differing times from the differentiation procedure, with or with out a prior purification stage of this people (Ditadi et?al. (bioskinrevive.com)
  • 2014). Jointly these variants in experimental strategies make it tough to reach apparent conclusions and consensus about the type and potential of HE cells. (bioskinrevive.com)
Reproducible2
  • This is an important step in placental biology, because it provides a reproducible cell culture model for the study of human placental development and disease. (ca.gov)
  • A major barrier to studying the pathogenesis, virus-host interactions, and effect of control measures to prevent and treat norovirus gastroenteritis has been the lack of a robust and reproducible cell culture system. (cdc.gov)
Research on embryon1
Maturation3
  • Moreover, during maturation in suspension culture, differentiating cells assembled in islet-like clusters, which expressed specific endocrine markers such as PDX1, SST, GCG and INS. (ox.ac.uk)
  • Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation. (ku.dk)
  • However, many of the mechanisms that govern their differentiation, maturation, and integration into the host circuitry remain elusive. (lu.se)
Gene expression2
  • Using an isogenic system, we demonstrate that TNT reprogramming can correct the transposable element overexpression and differential gene expression seen in conventional hiPS cells, and that TNT-reprogrammed hiPS and hES cells show similar differentiation efficiencies. (bvsalud.org)
  • The results showed that the space microgravity environment provided favorable conditions for the 3D growth and maintenance of stemness of mouse embryonic stem cells, and stem cells cultured in space showed a better 3D growth mode than on the ground and maintained a higher level of pluripotent gene expression. (pdnews.cn)
Therapy20
  • California voters received, instead, a TV campaign promising cures tomorrow for a host of diseases, some of which may never respond to stem cell therapy. (latimes.com)
  • Gene therapy and monoclonal antibodies didn't arrive with even a fraction of the hype surrounding embryonic stem cell research. (latimes.com)
  • This is the basic idea of cell therapy. (bayer.com)
  • Depending on the type of therapy, the cells are collected either from donors or from the individual patients. (bayer.com)
  • When it comes to the development of innovative cell therapy treatments, the development of pluripotent stem cells (PSCs) constitutes a quantum leap. (bayer.com)
  • By acquiring BlueRock Therapeutics (BlueRock) in 2019, Bayer ventured into PSC derived cell therapy. (bayer.com)
  • To create next-generation cell therapies, we are also incorporating gene editing with an aim to increase potency and persistence, further reduce immunogenicity, or build safeguard mechanisms into the cells, making cell therapy an even more powerful tool to help patients suffering from intractable diseases. (bayer.com)
  • The study was supported by São Paulo Research Foundation - FAPESP , and the results are published in Stem Cell Research & Therapy . (eurekalert.org)
  • Single cell manipulation is an important process in intracytoplasmic sperm injection (ICSI), pro-nuclei DNA injection, gene therapy, and other biomedical areas. (umd.edu)
  • Stem Cell Research & Therapy 14.1 (2023): 63. (bioinfor.com)
  • However, in recent years, stem cell therapy has emerged as a promising treatment option for TBI. (globalstemcelltherapy.com)
  • How stem cell therapy helps to treat Traumatic Brain Injuries? (globalstemcelltherapy.com)
  • Stem cell therapy for traumatic brain injuries (TBI) works by promoting the regeneration of damaged cells in the brain. (globalstemcelltherapy.com)
  • One of the benefits of stem cell therapy for TBI is its non-invasive nature. (globalstemcelltherapy.com)
  • Despite its potential benefits, stem cell therapy for TBI is still in the early stages of development, and there is limited evidence on its safety and efficacy in humans. (globalstemcelltherapy.com)
  • In addition, there are many factors that can affect the outcome of stem cell therapy, including the type of stem cells used, the method of delivery, and the stage of the injury. (globalstemcelltherapy.com)
  • Overall, stem cell therapy offers a promising new approach to treating TBI and its associated symptoms. (globalstemcelltherapy.com)
  • If you or a loved one is suffering from TBI, it's important to work with a doctor or specialist to determine the best course of action, which may include a combination of traditional medical treatments and alternative therapies like stem cell therapy. (globalstemcelltherapy.com)
  • One such idea is cell replacement therapy (CRT). (lu.se)
  • [ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)
Populations4
  • Moreover, single cell heterogeneity amongst stem cell populations may underlie important cell fate decisions. (jci.org)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • [ 2 ] In addition, some tumor types do not appear to adhere to the CSC concept at all [ 3 , 4 ] or may have multiple independent tumor-initiating cell populations. (medscape.com)
Proliferative1
  • Specific properties, such as proliferative and differentiation potential, are slightly different among DSCs. (bvsalud.org)
Occurs2
  • Cell differentiation occurs throughout life. (cusabio.com)
  • After a short period, capillary vasodilatation occurs secondary to local histamine release, and the cells of inflammation are able to migrate to the wound bed. (medscape.com)
Biology4
  • We have developed and renovated a 1400 sq. ft. shared-use stem cell laboratory called the Laboratory for Stem Cell Biology and Engineering. (ca.gov)
  • Past and current users of the Laboratory for Stem Cell Biology and Engineering include 52 researchers from 18 research groups, representing collaborations with 12 companies and 17 different universities and research institutes. (ca.gov)
  • Past and current users of the Laboratory for Stem Cell Biology and Engineering include 58 researchers (6 new researchers in the past year) from 18 research groups, representing collaborations with 12 companies and 17 different universities and research institutes. (ca.gov)
  • At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)
Osteoblasts2
  • It has also been shown that CHD1 is important in dictating the transcriptional landscape by promoting differentiation of osteoblasts, or differentiating bone cells. (wikipedia.org)
  • Among them, Runx2 is a key target gene affecting osteogenic activity, and Cbfa1 regulates the differentiation of MSCs into osteoblasts at the transcriptional level. (cusabio.com)
Types20
  • Particularly, pathological and toxicology modeling can be performed by the provision of physiological models for different human cell types. (grandviewresearch.com)
  • Human embryonic stem cells are so exciting because they provide a pathway of making many desperately-needed cell types for use in medicine," said Michael D. West, President & C.E.O. at ACT and an author on the paper. (news-medical.net)
  • Two biological replicates of Hi-C experiment and one replicate of CTCF ChIP-Seq experiment in embryonic stem cells and 4 other differentiated cell-types from H1 cell line. (nih.gov)
  • Cells are the building blocks of life - more than 200 highly distinct types make up every part of our bodies, ranging from brain and heart cells to bone and blood cells. (bayer.com)
  • Cell differentiation is the process where a cell changes from one type to many different types. (cusabio.com)
  • Acute and chronic wound management may also be advanced by incorporating stem or other cell types into biosynthetic 3D Matrices to enhance the ideal properties required for skin substitutes (10, 11, 12 and 13). (webmedcentral.com)
  • This kind of reprogramming makes it possible to reform all human cell types without the ethical restrictions related to using embryonic stem cells. (eurekalert.org)
  • The researchers proved that the iPSC cells thus obtained then had the capacity to reform all types of human cells. (eurekalert.org)
  • In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits. (rndsystems.com)
  • There are different types of stem cells that best can be described in the context of normal human development [ 23 ]. (biomedcentral.com)
  • It has been suggested that adults contain 20 different types of stem cells. (biomedcentral.com)
  • Moreover, TNT reprogramming enhances the differentiation of hiPS cells derived from multiple cell types. (bvsalud.org)
  • In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • Calcium signaling mediates five types of cell morphological changes to form neural rosettes. (creative-biolabs.com)
  • The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. (dattanibookagency.in)
  • Stem cells are unique in that they have the ability to differentiate into various cell types, including nerve cells, and can potentially replace damaged cells in the brain. (globalstemcelltherapy.com)
  • Both types of stem cells have shown promise in animal studies, but more research is needed to determine their safety and efficacy in humans. (globalstemcelltherapy.com)
  • When stem cells are infused into the bloodstream or directly implanted into the brain, they have the ability to differentiate into various cell types, including nerve cells. (globalstemcelltherapy.com)
  • Objective: the aim of this study was to conduct a literature review of the types of stem cells of dental origin and their applications in Dentistry. (bvsalud.org)