Human complement serum bactericidal activity serum bactericidal test. Medical search. Frequent questions

In H. influenzae , e (P4) stimulates production of bactericidal and protective antibodies and contributes to pathogenesis by facilitating acquisition of the essential nutrients heme and nicotinamide adenine dinucleotide (NAD). (biomedcentral.com)
Here, we tested the hypothesis that, like its homolog, H. ducreyi OmpP4 contributes to virulence and stimulates production of bactericidal antibodies. (biomedcentral.com)
Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. (southernbiotech.com)
The primary objective of this research work is to develop a serum bactericidal assay (SBA) for the quantification of bactericidal antibodies in human serum for P. aeruginosa (pSBA). (ijsr.net)
The serum contained antibodies to P. gingivalis as measured by enzyme-linked immunosorbent assay in this laboratory. (docksci.com)
In humans, it has high concentrations of nutrients and antibodies, but it is small in quantity. (health-mall.in)
Colostrum is high in carbohydrates, high in protein, high in antibodies, and low in fat (as human newborns may find fat difficult to digest). (health-mall.in)
Immunologic tests for identification of HIV (HTLV-III/LAV) antibodies. (bvsalud.org)

Hyperimmune mouse serum raised against purified, recombinant OmpP4 did not promote bactericidal killing of 35000HP or phagocytosis by J774A.1 mouse macrophages in serum bactericidal and phagocytosis assays, respectively. (biomedcentral.com)
Immune responses were assessed by serum bactericidal assays using human complement (hSBA). (worldpharmatoday.com)
FITC Anti-Human IgG 3 Hinge antibody for use in flow cytometry, immunohistochemistry, and western blot assays. (southernbiotech.com)
This provides a product that has low heterophile antibody activity for many applications, including use in bactericidal and opsonization assays. (pel-freez.com)
Suitable for functional antibody assays (SBA-Serum Bactericidal Assay, MOPA/OPA- Multiplexed /Opsonophagocytic Killing Assay) for vaccine evaluation. (pel-freez.com)

To verify that the point mutation caused a splicing defect, we tested wild-type and mutant mRNA substrates, containing 333 nt of the C8α intron 6/exon 7 boundary, in an in vitro splicing assay. (aai.org)
Interactions were tested in a solid phase assay, which led to the identification of 23 protein-protein interactions among which 20 are new. (biomedcentral.com)
Pooled serum from three healthy individuals was used for the opsonin in the assay. (docksci.com)
A microplate dilution assay was used to determine the antibacterial activity of green tea extract and EGCG against S. moorei . (biomedcentral.com)

Host defense against bacterial invasion depends on phagocytosis by polymorphonuclear granulocytes and the bactericidal effect of serum, mediated in large part by complement proteins. (medscape.com)
of 1.0 = 8 x 108 CFU/ml) opsonized in 10% human serum was added to Phagocytosis of bacteria by polymorphonuclear leukocytes (PMNLs) is accompanied by enhancement of PMNL oxidative metabolism. (docksci.com)

Human IgG subclass responses in relation to serum bactericidal and opsonic activities after immunization with three doses of the Norwegian serogroup B meningococcal outer membrane vesicle vaccine. (southernbiotech.com)

RNA sequencing was performed in primary human macrophages (Mφ) and polymorphonuclear neutrophils (PMNs) infected with a clinical strain of Brucella spp. (frontiersin.org)
Herein, we investigated the interactions between a panel of defined M. marinum LOS mutants that exhibited various degrees of truncation in the LOS structure, and human-derived THP-1 macrophages to address the potential of LOSs to act as pro- or avirulence factors. (biomedcentral.com)

Rat or human but not mouse sera were bactericidal against a Y. pestis ail mutant at 28C in vitro. (cdc.gov)
In vitro antiprotozoal activity of ethanolic extract of Egyptian propolis and a cysteine protease inhibitor, phenyl vinyl sulfone (PVS) on Blastocystis spp. (blogspot.com)
This is the first study to explore the inhibitory effects of compounds from P. frutescens on Trichophyton mentagrophytes and its mechanism of action coupled with the antifungal activity in vitro from network pharmacology, transcriptomics and proteomics. (biomedcentral.com)
In summary, our study demonstrates that PAR2 agonist acting alone can enhance a bactericidal response of human neutrophils Ureohydrolase and monocytes in vitro. (cgrpreceptor.com)
In vitro to evaluate the effect of ten 3-formylchromone derivatives on the formation of p-amyloid aggregates and tyrosinase activity. (eco-vector.com)

The Meningococcal Vaccines Work Group of ACIP reviewed available data on immunogenicity in high-risk groups, bactericidal antibody persistence after immunization, current epidemiology, vaccine effectiveness (VE), and cost-effectiveness of different strategies for vaccination of adolescents. (cdc.gov)
Complement, also known as alexin, cytase, or addiment, is generally defined as the portion of fresh blood serum or plasma which is not increased on immunization and which, when added to certain sensitized cells, combines with these sensitized cells and, under appropriate conditions, results in their destruction or death. (datapdf.com)

This antibody has a high affinity for CEA and shows no detectable reactivity to nonspecific cross-reacting antigen (NCA), biliary glycoprotein (BGP) and human polymorphonuclear leucocytes. (samatashkhis.com)

ACIP also recommends routine vaccination for persons at increased risk for meningococcal disease (i.e., persons who have persistent complement component deficiencies, persons who have anatomic or functional asplenia, microbiologists who routinely are exposed to isolates of N. meningitidis, military recruits, and persons who travel to or reside in areas in which meningococcal disease is hyperendemic or epidemic). (cdc.gov)
This report summarizes two new recommendations approved by ACIP: 1) routine vaccination of adolescents, preferably at age 11 or 12 years, with a booster dose at age 16 years and 2) a 2-dose primary series administered 2 months apart for persons aged 2 through 54 years with persistent complement component deficiency (e.g. (cdc.gov)

Laboratory studies of mother included an elevated white blood cell count of 15,900 cell/mm3 with 81% neutrophils, RPR of 1:4, consistent with previously treated syphilis, positive hepatitis C antibody and hepatitis B surface antigen after prior negative results, negative rapid HIV test and positive urine toxicology screen for cocaine. (pediatriconcall.com)

Low binding with NHba p0002 antisera is consistent with decreased susceptibility to anti-NHba bactericidal activity in persons vaccinated with MenB-4C, but the correlation between flow cytometric binding and hSBA response has not been established. (cdc.gov)
The hSBA responses one month after doses 2 and 3 against the four primary MnB test strains as defined by hSBA responses (titers ≥LLOQ) were 64.0%-99.1% and 87.1%-99.5%, respectively. (worldpharmatoday.com)
The hSBA responses to the 10 additional MnB test strains were 61.1%-100.0% and 75.1%-98.6% one month after dose 2 and 3, respectively. (worldpharmatoday.com)

Flea colonization studies using murine sera and Y. pestis KIM6(+) wild type, a ail mutant, and the ail/ail(+) control showed no differences in bacterial prevalence or numbers during the early stage of flea colonization. (cdc.gov)
Murine sera is not bactericidal. (cdc.gov)
Here we show that, in neutrophils, increases in Na + (high salt, HS) impair the ability of human and murine neutrophils to eliminate Escherichia coli and Staphylococcus aureus . (frontiersin.org)
We approached this issue with a small natural product derived oligosaccharide with TLR4 modulating activity that delicately balances the immune system and the production of anti-inflammatory and pro-inflammatory cytokines in a murine model of peritonitis. (nature.com)

Sort By: Guinea Pig Complement. (carlosvaldivieso.es)
Existing views on the chemical nature and the functions of each component of guinea pig complement are summarized. (datapdf.com)

Interleukin-1: A beneficial 01-APR-1988 known by products, tissues, and transitional reasons which has sites and is their complement to adults or questions. (siriuspixels.com)

This review paper will 1) present an overview of human physiological responses to cold exposure, 2) present the human studies examining the effects of cold exposure on immune responses, and 3) summarize recent experiments from our laboratories examining the effects of exercise and fatigue on immune responses during subsequent cold exposure. (researchgate.net)
12] However, the findings concerning the effects of cold exposure on human immune function are inconsistent. (researchgate.net)
they have receptors for the crystallizable fragment (Fc) region of immunoglobulin (Ig) G and for complement, which enable them to bind with immune complexes and present the complex to B cells in germinal centers of secondary lymphoid organs. (msdmanuals.com)
Taken together, this study provides for the first time an in-depth analysis of the transcriptomic alterations that take place in human phagocytes upon infection, and in peripheral blood immune populations during active disease. (frontiersin.org)
It can also induce granulomatous infection in humans called "fish tank disease" [ 2 ] and, as other nontuberculous mycobacteria, is responsible for opportunistic infections in immune-deficient patients [ 4 ]. (biomedcentral.com)
The human body incorporates innate immune responses evolved in the earliest multicellular organisms. (ishinobu.com)

ELISA positive sera were pSBA positive. (ijsr.net)

A deficiency of any of the C5-C9 terminal C components is associated with loss of serum bactericidal activity and increased risk of meningococcal disease ( 1 , 2 ). (aai.org)
After licensure, additional data on bactericidal antibody persistence, trends in meningococcal disease epidemiology in the United States, and VE have indicated many adolescents might not be protected for more than 5 years. (cdc.gov)

pSBA antibody driven with no interference with endogenous complement. (ijsr.net)
This study assessed endogenous knowledge and impact of human disturbance on the abundance of two underutilized wild fruit tree species: Drypetes floribunda (Mu¨ll. (researchgate.net)

We designed a medium throughput approach to systematically test for interactions between these pneumococcal surface proteins and host proteins (extracellular matrix proteins, circulating proteins or immunity related proteins). (biomedcentral.com)
The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. (nih.gov)
Among the different complement proteins, the opsonins C3 and C4 have a protector thioester radical. (samatashkhis.com)
BOB.1 mission is to increase transcriptional activity of such proteins. (samatashkhis.com)

Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. (cusabio.com)
Recombinant full length human. (samatashkhis.com)

1888 - Identification of bacterial toxins (diphtheria bacillus) (Pierre Roux and Alexandre Yersin) 1888 - Bactericidal action of blood (George Nuttall) 1890 - Demonstration of antibody activity against diphtheria and tetanus toxins. (wikipedia.org)

This study demonstrated Anti-pseudomonas functional activity in human serum. (ijsr.net)
C5--C9, properidin, factor H, or factor D) and functional or anatomic asplenia, and for adolescents with human immunodeficiency virus (HIV) infection. (cdc.gov)

The importance of this observation is that it identifies a protective niche for the growth of serum sensitive and nonsensitive Y. pestis strains. (cdc.gov)
In pioneering experiments in the 1930s, Fothergill and Wright demonstrated that blood obtained from children aged 3 months to 3 years lacked bactericidal activity against type b strains, whereas the blood of neonates, older children, and adults was bactericidal. (medscape.com)
The study primary endpoint assessed the percentage of subjects with a response to four primary MnB test strains (N=1210-1266), representative of prevalent MnB strains, and the secondary endpoint assessed responses to 10 additional MnB test strains in a population subset (N=266-281). (worldpharmatoday.com)

Yersinia pestis Δail Mutants Are Not Susceptible to Human Complement Bactericidal Activity in the Flea. (cdc.gov)
Like other fluoroquinolone anti-infectives, ciprofloxacin inhibits DNA synthesis in susceptible organisms via inhibition of the enzymatic activities of 2 members of the DNA topoisomerase class of enzymes, DNA gyrase and topoisomerase IV. (antiinfectivemeds.com)

Haemophilus ducreyi , which causes the sexually transmitted disease chancroid, expresses a number of lipoproteins during human infection. (biomedcentral.com)
Natural disease epidemiology in humans and non-human primate infection studies show complete protection from re-infection with a homologous Shigella species. (biomedcentral.com)
Cytomegalovirus (CMV) Infection Cytomegalovirus (CMV, human herpesvirus type 5) can cause infections that have a wide range of severity. (msdmanuals.com)

Complement-dependent bactericidal activity, a vital component of innate immunity, develops upon assembly of the membrane attack complex from the terminal C components, C5-C9. (aai.org)

A novel oligosaccharide compound AVR-25 selectively binds to the TLR4 protein (IC 50 = 0.15 µM) in human peripheral blood monocytes and stimulates IL-10 production. (nature.com)

Finally, a variant of Ail (Ail(F100V E108_S109insS)) from a human serum-sensitive Y. pestis subsp. (cdc.gov)
In humans C. fetus subsp fetus rarely causes invasive disease except in immunocompromised patients including pregnant women and neonates. (pediatriconcall.com)

LOUIS PILLEMER independently showed that defibrinated blood, peptone blood, and blood serum exert measurable destructive influence upon bacteria. (datapdf.com)

To test whether OmpP4 was necessary for virulence in humans, eight healthy adults were experimentally infected. (biomedcentral.com)

Protein content was determined by Bradford's method (5) with bovine serum albumin as the standard. (docksci.com)
Human and bovine colostrums are thick, sticky and yellowish. (health-mall.in)
Bovine colostrum for human consumption is collected from cows during the first 24 hours after birth. (health-mall.in)

MenB-FHbp contains FHbp from subfamilies A and B. Although the subfamily A FHbp contained in MenB-FHbp (A05) is not the same peptide allele as that in the outbreak strain (A22), some level of cross-reactivity is expected based on prior testing by the manufacturer ( 2 ). (cdc.gov)
However, based on flow cytometry, the strain had relatively low expression of FHbp, which would be expected to decrease susceptibility to anti-FHbp bactericidal activity ( 4 ). (cdc.gov)

However, a higher rate of acute toxicity (severe cutaneous toxicity and need for nutritional support) was found for RT-Cx, with 11% of harmful death and 13% of discontinuation rate of RT 0% of CRT group (33.3%, 15.1%) were detected.51 Recently, two randomized phase?III tests, RTOG 1016 and De-ESCALaTE, investigated the substitution of cisplatin with Cx in individuals with advanced HPV? (siamtech.net)
These antigens were expressed and probed with acute and convalescent serum from microbiologically confirmed Shigella infections. (biomedcentral.com)
The concentration of several inflammatory mediators was measured in the serum of these patients, and levels of IFN-γ, IL-1β and IL-6 were found significantly increased before the treatment of acute brucellosis. (frontiersin.org)
Limited information is available on the acute toxicity of ciprofloxacin in humans. (antiinfectivemeds.com)
Consecutively, C4d is a stable split product remnant of classical complement activation and an established marker of antibody-mediated acute renal allograft rejection. (samatashkhis.com)

To prevent accidental overdose, oral doses for humans should not exceed 350 mg/kg (21 g/60 kg). (drugs.com)

A decrease in serum C-reactive protein (CRP) and bacterial colony forming unit (CFU) confirmed improved bacterial clearance. (nature.com)

Description: Rabbit Complement, Lyophilized, 3-4 week, Physical State: Dry Powder, Hemolytic titer: greater than or equal to 1:16, Endotoxin: less than or equal to 1. (carlosvaldivieso.es)

These findings highlight the potential antiprotozoal activity of propolis extract as a potent natural alternative for MTZ in treatment of blastocystosis. (blogspot.com)
The key findings of the present study manifested that rosmarinic acid, a medicinal compound extracted from P. frutescens , had pharmacological activity in inhibiting the growth of Trichophyton mentagrophytes by affecting its enolase expression to reduce metabolism. (biomedcentral.com)

Ail confers serum resistance in humans and is a critical virulence factor of Y. pestis, the causative agent of plague. (cdc.gov)
Among identified virulence factors is the attachment invasion locus protein, Ail, that is required to protect Y. pestis from serum complement in all mammals tested except mice. (cdc.gov)

Although neutrophils play an important role in fighting intruding invaders, the impact of increased Na + on the antimicrobial activity of neutrophils remains elusive. (frontiersin.org)
Stimulation of neutrophils with phorbol-12-myristate-13-acetate (PMA) overcame high salt-induced impairment in ROS production and restored antimicrobial activity of neutrophils. (frontiersin.org)
Hence, the effects of the inhibitors of signalling molecules at MCP-1 release were not studied after IFN-γ stimulation of human monocytes and neutrophils. (cgrpreceptor.com)
Altogether, the results of our experiments allowed us to suggest a possible scheme of signalling events involved in the enhancement of MCP-1 secretion triggered after combined stimulation of human neutrophils and monocytes GSK-3 inhibition with PAR2-cAP and IFN-γ (Fig. 6a,b). (cgrpreceptor.com)

Complement components deposited rapidly on the Y. pestis surface as measured by immunofluorescent microscopy. (cdc.gov)
This is accomplished mainly by inhibiting the activation or uptake of complement components, especially C3b. (medscape.com)
Received March 4 , 1943 Complement is considered to be a chemical system composed of multiple components. (datapdf.com)
It is the purpose of this review to consider complement as a chemical system composed of multiple components and to summarize, in the light of recent advances, existing views on the chemical nature and the function of each ofthe components of guinea pig ~omplement. (datapdf.com)
Oxygen metabolites such as superoxide (027), hydrogen peroxide (H202), singlet oxygen ('02), and hydroxy radical ('OH) are major components of the bactericidal activity of PMNLs (14). (docksci.com)
Human milk contains special components, called growth modulators, that help the premature baby's digestive system adjust to oral feedings. (health-mall.in)

Authors are thankful to the department of Biochemistry of Muthayammal College of Arts and Science, Rasipuram, Tamil Nadu and Dr.B.Duraiswamy, Department of pharmacognosy, ooty, Tamil Nadu for their encouragement and technical support in testing the extracts for activity. (peptidecost.com)

However, AlpaWash and Polysporin presented some additional benefits- anti-inflammatory activity, measured using myeloperoxidase and histological count, as well as fibroplasia and hydroxyproline production, suggesting that skin with a better quality could be formed following these two treatments. (blogspot.com)

Premature babies tend to fare better on human colostrum than commercial infant formulas. (health-mall.in)
Research indicates that premature babies fed formula tend to vomit more and continue tube feeding longer than those fed human colostrum and breast milk. (health-mall.in)

They are able to activate complement, which causes selective deposition of C3b onto LPS molecules at sites distant from the bacterial cell membrane. (medscape.com)

Six antigens (FepA, EmrK, FhuA, MdtA, NlpB, and CjrA) were identified in in vivo testing as capable of producing binding IgG and complement-mediated bactericidal antibody. (biomedcentral.com)
Both classic-pathway and alternate-pathway complement activation have been described, but the latter, which does not require the presence of immunoglobulins directed against bacterial antigens, appears to be the more active pathway in K pneumoniae infections. (medscape.com)

Multiple lots of baby rabbit serum complement were screened for potency and nontoxicity prior to use as an exogenous complement source in the OPA (catalog no. (carlosvaldivieso.es)
The Pel-Freez Rabbit reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (carlosvaldivieso.es)
anti-human CEA rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. (samatashkhis.com)
anti-human Caspase 3 rabbit polyclonal antibody purified from rabbit anti-serum by Protein A chromatography. (samatashkhis.com)

However, in the flea vector, the serum protective properties of Ail were not required. (cdc.gov)

Although chemically different from the LPS of the Enterobacteriaceae, the biological activity of Hib LPS appears to be similar to that of other gram-negative endotoxins. (medscape.com)
A densities assessment suggests a negative effect of human disturbance on the abundance of both species. (researchgate.net)
DNA gyrase introduces negative superhelical twists in DNA, an activity important for initiation of DNA replication. (antiinfectivemeds.com)

In this study, we asked, is bactericidal sera from humans active in Y. pestis colonized fleas We found it was not. (cdc.gov)
In spite Alisertib of these limitations, the results of our study provide a practical means to address important hypotheses in human subjects with T1D. (nartsignaling.com)
In studies in humans, several properties are taken into consideration to determine the appropriateness of the group of patients accessed for a study. (cgrpreceptor.com)

Hence, we conclude that high salt-impaired PHOX activity results in diminished antimicrobial activity. (frontiersin.org)
for best results, thaw complement on ice or in a cold water bath. (pel-freez.com)
However, extrapolating these outcomes to medical use is troublesome because these research should control for results corresponding to blinking, tear dilution and turnover, and buffering capabilities of the human eye. (atpobtvs.com)
A survey was conducted using a questionnaire from April 1 to June 30, 2021, among travelers who came for their COVID-19 test at the Institut Pasteur of Côte d'Ivoire.Results. (bvsalud.org)

Accordingly a file in the total of the deg has below substitute the Master-Signifier( or contrast inundation) but is a collection along the tofu test to a Specific sign carried. (onlinemedsupplies.com)

The peculiar complexity and extreme perishability of complement have often discouraged the initiation or continuance of promising experiments. (datapdf.com)

Moreover, rottlerin, when applied alone, enhanced MCP-1 secretion by human monocytes (Fig. 5c). (cgrpreceptor.com)
The treatment of human monocytes with IFN-γ yielded no significant changes in MCP-1 levels (Fig. 3c). (cgrpreceptor.com)

Felix d'Herelle first coined the term "bacteriophage" ( 4 ), literally meaning "bacterium eater," and began using phages to treat bacterial infections in human patients ( Fig. 1 ). (asm.org)
K pneumoniae and K oxytoca are the 2 members of this genus responsible for most human infections. (medscape.com)

SOD is thought to protect the cells of Nocardia asteroides (9), Listeria monocytogenes (24), and Shigella flexneri (10) from phagocytic killing, whereas catalase activity has been suggested to protect Staphylococcus aureus (12) and Neisseria gonorrhoeae (3). (docksci.com)

Similarly, flea studies with human blood showed Ail was not required for serum resistance. (cdc.gov)

It is also an occasional opportunistic human pathogen. (biomedcentral.com)

32, 33] Applying these methods with this selection of epitopes would provide an excellent tool to measure the frequencies, phenotypes and dynamics of autoreactive T cells in human subjects. (nartsignaling.com)

By going through up-to-date reports and, whenever possible, human clinical trials, we examine the versatility of phage therapy. (asm.org)
Supporting the EAE data, Palbociclib clinical trial the authors identified elevated pretreatment serum levels of IL-17F in a small subgroup of IFN-β non-responders. (nartsignaling.com)
This article summarizes recent advances in the clinical characterization of CHS patients, provides updates on promising new testing methods, and focuses on specific therapeutic approaches. (biomedcentral.com)
The project has been reviewed and approved by the Committee on Human Rights Related to Research Involving Human Subjects of Kyiv City Clinical Emergency Hospital (Kyiv, Ukraine), based on the Declaration of Helsinki. (wjgnet.com)

The disease is transmitted to humans by consumption of unpasteurized milk and dairy products or by occupational contact with infected animals. (frontiersin.org)

The human gene, CHS1 , was originally called LYST for lysosomal trafficking regulator gene ( LYST , OMIM #606897). (biomedcentral.com)

Identification of new citrulline-specific autoantibodies, which bind to human arthritic cartilage, by mass spectrometric analysis of citrullinated type II collagen. (southernbiotech.com)

Anatomy2

Organisms11

Diseases4

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes9

Information Science1

Antibodies8

Assays5

Assay4

Phagocytosis2

Serogroup B meningococcal1

Macrophages2

Vitro5

Immunization2

Polymorphonuclear1

Persistent complement component2

Antigen1

HSBA3

Murine4

Guinea Pig Complement2

Tissues1

Immune6

ELISA1

Meningococcal disease2

Endogenous2

Proteins4

Recombinant2

Antibody activity1

Functional2

Strains3

Susceptible2

Infection3

Innate immunity1

Stimulates1

Subsp2

Bacteria1

Adults1

Bovine3

FHbp2

Acute5

Doses1

Decrease1

Physical State1

Findings2

Virulence2

Neutrophils4

Components6

Extracts1

Anti-inflammatory activity1

Premature2

Activate1

Antigens2

Rabbit4

Protective1

Negative3

Study3

Results4

Tofu1

Initiation1

Monocytes2

Infections2

Phagocytic1

Blood1

Pathogen1

Epitopes1

Clinical4

Unpasteurized milk1

Gene1

Bind1