BUB1InteractsMediates the interactionCDC20KinetochoreBUBR1Beta-propeller structureKnl1KinaseRegulateKinetochoresInteractionComplement C1q bindingMass spectrometryMotifsSpecificityMad2Hydrodynamic propertiesEffectorSpindleAntibodyWD40Source:HGNCChromosomeSaccharomycesBUB1BUbiquitousSubunitCrucialEukaryotesPolyclonalInteractionsUbiquitinEndogenousArrestComplexSerineVitroTrypsinRepeatsRabbitExperimentsMicrobiomeMicrobiotaCellularSpeciesReceptorTherapeuticTranscriptionCellCellsHomoFamilySubsequent
BUB111
- while Bub3 specifically binds Mad3 and Bub1, Rae1 has more promiscuous binding as it binds both the nuclear pore complex and Bub1. (wikipedia.org)
- This indicates a similarity in interaction of Bub3 and Rae1 with Bub1. (wikipedia.org)
- Bub3 has been shown to form complexes with Mad1-Bub1 and with Cdc20 (the interaction of which does not require intact kinetochores). (wikipedia.org)
- Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. (wikipedia.org)
- In both Saccharomyces cerevisiae and metazoans, Bub3 has been shown to bind BubR1 and Bub1. (wikipedia.org)
- The components that are essential for the spindle assembly checkpoint in yeast have been determined to be Bub1, Bub3, Mad1, Mad2, Mad3, and the increasingly important Mps1 (a protein kinase). (wikipedia.org)
- BUB3 forms a complex with BUB1 (BUB1/BUB3 complex) to inhibit the anaphase-promoting complex or cyclosome (APC/C) as soon as the spindle-assembly checkpoint is activated. (wikipedia.org)
- BUB3 also phosphorylates: CDC20 (activator) and thereby inhibits the ubiquitin ligase activity of APC/C. MAD1L1, which usually interacts with BUB1 and BUBR1, and in turn the BUB1/BUB3 complex interacts with MAD1L1. (wikipedia.org)
- Met-Glu-Leu-Thr (MELT) repeats, ubiquitous in evolution, recruit the Bub3/Bub1 complex in a phosphorylation- dependent manner. (unimib.it)
- The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. (icr.ac.uk)
- The BUB1-BUB3 complex interacts with MAD1L1. (icr.ac.uk)
Interacts3
- The middle region of human BubR1 (BubR1M) also interacts with Cdc20, but the nature and function of this interaction are not understood. (elsevierpure.com)
- Interacts with BUB3 and KNL1. (icr.ac.uk)
- The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I. (separase.com)
Mediates the interaction2
- The structural data suggested that Bub3 acts as a platform that mediates the interaction of SAC protein complexes. (wikipedia.org)
- Background The KMN network, a ten-subunit protein complex, mediates the interaction of kinetochores with spindle microtubules and recruits spindle assembly checkpoint (SAC) constituents to halt cells in mitosis until attainment of sister chromatid biorientation. (unimib.it)
CDC205
- At unattached kinetochores, a complex consisting of BubR1, Bub3, and Cdc20 interact with the Mad2-Cdc20 complex to inhibit the APC, thus inhibiting the formation of active APCCdc20. (wikipedia.org)
- When the SAC is activated, the production of the Bub3-Cdc20 complex is activated. (wikipedia.org)
- Mutation of one of these motifs has indicated an impaired ability of Bub3 to interact with Mad2, Mad3, and Cdc20. (wikipedia.org)
- Unattached or tensionless kinetochores activate the checkpoint and enhance the production of the mitotic checkpoint complex (MCC) consisting of BubR1, Bub3, Mad2, and Cdc20. (elsevierpure.com)
- Here we identify two critical motifs within BubR1M that contribute to Cdc20 binding and anaphase-promoting complex/cyclosome inhibition: a destruction box (D box) and a phenylalanine-containing motif termed the Phe box.ABubR1 mutant lacking these motifs is defective in MCC maintenance in mitotic human cells but is capable of supporting spindle-checkpoint function. (elsevierpure.com)
Kinetochore5
- As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorientation. (wikipedia.org)
- After kinetochore attachment is complete, the spindle checkpoint complexes (including the BubR1-Bub3) experience a decrease in concentration. (wikipedia.org)
- Another function of BUB3 is to promote correct kinetochore-microtubule (K-MT) attachments when the spindle-assembly checkpoint is active. (wikipedia.org)
- Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. (icr.ac.uk)
- Fig. 1 ), which in turn attracts to the kinetochore other SAC proteins that collectively prevent mitotic progression ( 3 ). (rupress.org)
BUBR11
- The protein phosphatases PP1 and PP2A-B56 are recruited to kinetochores through binding to KNL1 and BUBR1, respectively, and are thought to silence the SAC through dephosphorylation of the MELT repeats of KNL1, thus antagonizing MPS1 activity ( Fig. 1 ). (rupress.org)
Beta-propeller structure2
- The crystal structure of Bub3 indicates a protein of the seven-bladed beta-propeller structure with the presence of WD40 repeats, with each blade formed by four anti-parallel beta sheet strands that have been organized around a tapered channel. (wikipedia.org)
- WD40 repeats usually assume a 7-8 bladed beta-propeller fold, but proteins have been found with 4 to 16 repeated units, which also form a circularised beta-propeller structure. (embl.de)
Knl12
Kinase5
- A-kinase interacting protein 1 [Source. (gsea-msigdb.org)
- CDC28 protein kinase regulatory subuni. (gsea-msigdb.org)
- Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. (icr.ac.uk)
- The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. (utsouthwestern.edu)
- Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. (utsouthwestern.edu)
Regulate2
- Members of this family include the signal-transducing G protein beta subunit, as well as other proteins that regulate signal transduction, transcription, pre-mRNA splicing, cytoskeletal organization, and vesicular fusion. (embl.de)
- Core fucosylation and O -GlcNAcylation are the two most famous protein glycosylation modifications that regulate diverse physiological and pathological processes in living organisms. (mcw.edu)
Kinetochores2
- Centromere-associated protein-E (CENP-E) is an essential mitotic kinesin that is required for efficient, stable microtubule capture at kinetochores. (rupress.org)
- Silencing of the spindle assembly checkpoint involves two protein phosphatases, PP1 and PP2A-B56, that are thought to extinguish checkpoint signaling through dephosphorylation of a checkpoint scaffold at kinetochores. (rupress.org)
Interaction3
- SETD1A function in leukemia is mediated through interaction with mitotic regulators BuGZ/BUB3. (antibodiesinc.com)
- Present wireless systems have some drawbacks such as interaction profile and protein-protein sharing matrix. (jeckefairsuchung.com)
- Taking into account a crystal structure of Mtr4 interacting with a truncated, short Trf4 fragment (interaction of Mtr4 and short Air2 fragment is shown as well), one can argue for ring-interaction between the phenylalanine position and a tyrosine on the interacting protein (Mtr4). (eu.org)
Complement C1q binding1
- complement C1q binding protein [Source. (gsea-msigdb.org)
Mass spectrometry2
- Then, the enzymatic reaction with galactose oxidase was utilized to specifically oxidize O-GalNAc, but not O -GlcNAc, generating the different tags between glycopeptides with O -GlcNAc and O-GalNAc that can be easily distinguishable by mass spectrometry (MS). Among O -GlcNAcylated proteins commonly identified in three types of human cells, those related to transcription and RNA binding are highly enriched. (mcw.edu)
- Hahne H, Sobotzki N, Nyberg T, Helm D, Borodkin VS, van Aalten DM, Agnew B, Kuster B. Proteome wide purification and identification of O -GlcNAc-modified proteins using click chemistry and mass spectrometry. (mcw.edu)
Motifs1
- Microsatellites (MSI) are nucleotide repeats, repetitive motifs of 1 to 6 nucleotides, scattering widespread the human genome ( Ellegren 2004 ). (medium.com)
Specificity1
Mad21
- Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. (wikipedia.org)
Hydrodynamic properties1
Effector1
- The mechanisms of DNA repair is closely coupled with the DNA damage response (DDR), which involves the recruitment and localization within distinct nuclear foci of DNA damage sensors, mediators, transducers and effector proteins ( Polo and Jackson 2011 ). (medium.com)
Spindle1
- Bub3 is a crucial component in the formation of the mitotic spindle assembly complex, which forms a complex with other important proteins. (wikipedia.org)
Antibody2
- Bulk Order Inquiry for Anti-Green Fluorescent Protein Antibody ------- (please add any order requirements, including desired quantity, timing, etc. (antibodiesinc.com)
- Polyclonal antibodies offers greater sensitivity for detecting proteins that are present in low expression levels, as the target protein will bind more than one antibody molecule on its multiple epitopes (this is less advantageous for quantification experiments). (cusabio.com)
WD406
- Rae1 (an mRNA export factor), another member of the WD40 protein family, shows high sequence conservation with that of Bub3. (wikipedia.org)
- several WD40-containing proteins act as key regulators of plant-specific developmental events. (embl.de)
- There are 1918936 WD40 domains in 345139 proteins in SMART's nrdb database. (embl.de)
- Taxonomic distribution of proteins containing WD40 domain. (embl.de)
- The complete taxonomic breakdown of all proteins with WD40 domain is also avaliable . (embl.de)
- Click on the protein counts, or double click on taxonomic names to display all proteins containing WD40 domain in the selected taxonomic class. (embl.de)
Source:HGNC2
- centromere protein A [Source:HGNC Symb. (gsea-msigdb.org)
- BCL2 related protein A1 [Source:HGNC S. (gsea-msigdb.org)
Chromosome3
- Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. (icr.ac.uk)
- We recently demonstrated that mild DNA replication stress typically seen in cancer cells causes premature centriole disengagement in untransformed mitotic human cells, leading to transient multipolar spindles that favour chromosome missegregation. (bvsalud.org)
- Targeted TPX2 increases chromosome missegregation and suppresses tumor cell growth in human prostate cancer. (separase.com)
Saccharomyces1
- The duplication of the polymerase Trf and the zinc knuckle protein Air arose due to a whole genome duplication event in Saccharomyces cerevisiae ( 15004568 ). (eu.org)
BUB1B1
- BUB3 has been shown to interact with BUB1B, HDAC1 and Histone deacetylase 2. (wikipedia.org)
Ubiquitous1
- GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. (antibodiesinc.com)
Subunit1
Crucial1
- BACKGROUND: Cell division cycle associated 2 (CDCA2), a member of the cell division cycle associated proteins (CDCA) family, is crucial in the regulation of cell mitosis and DNA repair. (bvsalud.org)
Eukaryotes2
- though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. (wikipedia.org)
- WD-repeat proteins are a large family found in all eukaryotes and are implicated in a variety of functions ranging from signal transduction and transcription regulation to cell cycle control and apoptosis. (embl.de)
Polyclonal1
- Briefly, Control MP1 were fabricated from Vandetanib trifluoroacetate poly(lactic-co-glycolic acid) (PLGA) and Rabbit Polyclonal to MYL7 conditioned media of human CSCs which were isolated from human hearts using the cardiosphere method as previously described13,14 (Supplementary Fig. 1). (cckn-ia.org)
Interactions2
- Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions. (antibodiesinc.com)
- Host-microbial interactions in the human microbiota. (eviematilda.art)
Ubiquitin1
- denticleless E3 ubiquitin protein liga. (gsea-msigdb.org)
Endogenous1
- Such observations prompted us to propose that in addition to cytogenetic parameters, the levels of endogenous GSH and p53 protein could act as effective biomarkers for RAN chewers [ 8 ]. (biomedcentral.com)
Arrest1
- LCA significantly up-regulated p53 protein and gene expression, thereby inducing apoptosis and cycle arrest. (bvsalud.org)
Complex2
- in this arrangement, Bub3 acts as a key component of the SAC in the formation of an inhibitory complex. (wikipedia.org)
- The TRAMP complex consists of a RNA helicase (MTR4), a poly(A)polymerase (Trf4 or Trf5) and a RNA-binding zinc knuckle protein (Air1 or Air2). (eu.org)
Serine1
- arginine and serine rich protein 1 [So. (gsea-msigdb.org)
Vitro3
- However, the only studied example of a WD-repeat protein, G beta, synthesized in vitro in a rabbit reticulocyte lysate, is unable to fold into a native structure without its partner protein G gamma. (embl.de)
- It is not known whether all WD-repeat proteins are unable to fold when synthesized in an in vitro system. (embl.de)
- to develop in vitro human-based models for molecular and pre-clinical studies. (ucdavis.edu)
Trypsin2
- Our working definition of folding was that the proteins from globular, trypsin-resistant structures because, except for G beta gamma, their functions are not known or cannot be assayed in reticulocyte lysates. (embl.de)
- On-resin proteolysis using trypsin followed by LC-MS/MS afforded the identification of around 1500 O -GlcNAc proteins from a single cell line. (mcw.edu)
Repeats2
Rabbit1
- Find C10ORF96 Antibodies for a variety of species such as anti-Human C10ORF96, anti-Guinea Pig C10ORF96, anti-Rabbit C10ORF96. (antibodies-online.com)
Experiments1
- The proteins recognized in our experiments are presented in Extra file one Table S1 and Additional file two Table S2. (pafr-inhibitor.com)
Microbiome3
- Fecal microbiota transplant overcomes resistance to diet-induced obesity in germ-free (GF) model organisms is that the human microbiome is distinct from colocalized white subjects and connected to metabolic health. (jeckefairsuchung.com)
- Gut microbiome pattern reflects healthy ageing and predicts survival in humans. (eviematilda.art)
- The human microbiome is altered in aging mice. (eviematilda.art)
Microbiota1
- Sex differences how to buy minipress in usa in the human microbiota. (jeckefairsuchung.com)
Cellular3
- The post-translational modification of proteins with N-acetylglucosamine ( O -GlcNAc) is involved in the regulation of a wide variety of cellular processes and associated with a number of chronic diseases. (mcw.edu)
- To demonstrate the practical utility of the developed approach, we studied the global effects of the O -GlcNAcase inhibitor GlcNAcstatin G on the level of O -GlcNAc modification of cellular proteins. (mcw.edu)
- About 200 proteins including several key players involved in the hexosamine signaling pathway showed significantly increased O -GlcNAcylation levels in response to the drug, which further strengthens the link of O -GlcNAc protein modification to cellular nutrient sensing and response. (mcw.edu)
Species2
- Our range of C-JUN antibodies provides specific detection of C-JUN across a broad spectrum of samples, including Human, Mouse, Rat, Chicken, Cow, and numerous other species. (antibodies-online.com)
- Find C-JUN Antibodies for a variety of species such as anti-Human C-JUN, anti-Mouse C-JUN, anti-Rat C-JUN. (antibodies-online.com)
Receptor1
- adhesion G protein-coupled receptor E5. (gsea-msigdb.org)
Therapeutic2
- These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans. (antibodiesinc.com)
- However, these sporadic events could not explain the therapeutic benefits seen in animal models and human trials7,8. (cckn-ia.org)
Transcription1
- In the present study we used Affymetrix oligonucleotide microarrays to produce gene transcription profiles for the major leukocyte types in humans. (gsea-msigdb.org)
Cell6
- Rubella virus tropism and single-cell responses in human primary tissue and microglia-containing organoids. (antibodiesinc.com)
- Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons. (antibodiesinc.com)
- Identification of cell types in multiplexed in situ images by combining protein expression and spatial information using CELESTA reveals novel spatial biology. (microvolution.com)
- CMMPs (derived from human cells) do not Vandetanib trifluoroacetate stimulate T-cell infiltration in immuno-competent mice. (cckn-ia.org)
- CMMPs (derived from human cells) did not stimulate T-cell infiltration in immuno-competent mice, suggesting their excellent safety profile. (cckn-ia.org)
- Among glycoproteins exclusively in Jurkat cells, those involved in human T-lymphotropic virus type 1 (HTLV-1) infection are overrepresented, which is consistent with the cell line source and suggests that protein O -GlcNAcylation participated in the response to the virus infection. (mcw.edu)
Cells5
- CMMPs carry similar secreted proteins and membranes as genuine cardiac stem cells do. (cckn-ia.org)
- Xu S, Zheng J, Xiao H, Wu R. Simultaneously Identifying and Distinguishing Glycoproteins with O -GlcNAc and O-GalNAc (the Tn Antigen) in Human Cancer Cells. (mcw.edu)
- Glycoproteins with diverse glycans are essential to human cells, and subtle differences in glycan structures may result in entirely different functions. (mcw.edu)
- Furthermore, glycoproteins with the Tn antigen have different subcellular distributions in different cells, which may be attributed to the distinct mechanisms for the formation of protein O-GalNAcylation. (mcw.edu)
- In the present study, we demonstrate a significantly improved O -GlcNAc protein enrichment procedure, which exploits metabolic labeling of cells by azide-modified GlcNAc and copper-mediated Click chemistry for purification of modified proteins on an alkyne-resin. (mcw.edu)