• The primary barriers for invading respiratory pathogens are the airway epithelial cells of the respiratory tract and antimicrobial peptides produced by these cells. (cdc.gov)
  • Significant downregulation of β-defensin1 gene ( DEFB1 ) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • Primary human bronchial epithelial cells HBEp cells. (cdc.gov)
  • In this study, the human bronchial epithelial cells (16HBE) were treated five times with 1 ?M benzo(a)pyrene (BaP), followed by 2 passages culture, and thein vitroBaP-induced malignant transformation of 16HBE cells was established. (eurekamag.com)
  • In this study, we aimed to assess host differentially expressed gene signatures in respiratory tract epithelial cells after influenza A virus pdmH1N1 or H7N9 infection. (aacc.org)
  • In vitro models of RV infection of BEAS-2B and primary normal human bronchial epithelial (NHBE) cells were used. (nih.gov)
  • BUD and FORM suppress RV-induced chemokines and growth factors in bronchial epithelial cells in a concentration-dependent, synergistic or additive manner. (nih.gov)
  • Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)
  • In this study, we utilized primary normal human bronchial epithelial (NHBE) cells that are cultured at an air-liquid interface (ALI) to mimic the in vivo airway characteristics 30 . (nature.com)
  • The interaction between epithelial and stromal cells through soluble factors such as cytokines plays an important role in carcinogenesis. (psu.edu)
  • Methods: Primary human bronchial epithelial (HBE) cells cultured at air-liquid interface were treated with exogenous TGF-β1, TGF-β2, and/or a Th2 cytokine, interleukin (IL)-13. (manchester.ac.uk)
  • Inhaled fluticasone propionate (FP) is widely used to reduce pulmonary inflammation in chronic obstructive pulmonary disease, but the potential effects of FP on airway epithelial cells from patients with cystic fibrosis (CF) are unknown. (ersjournals.com)
  • In CF disease, a nonregulated inflammatory lung response occurs through exaggerated nuclear factor (NF)-κB activation and elevated pro-inflammatory cytokines production by airway epithelial cells. (ersjournals.com)
  • To determine whether FP reduces cytokine production in bronchial epithelial cells via NF-κB, the authors investigated the nonstimulated and the Pseudomonas aeruginosa lipopolysaccharide (LPS) stimulated production of NF-κB-dependent interleukin (IL)-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) along with the activation of NF-κB in non-CF and CF human bronchial gland epithelial cells. (ersjournals.com)
  • It was demonstrated that a relevant concentration of FP (10 −8 M) inhibited constitutive and P. aeruginosa LPS-induced IL-6 and IL-8 production of non-CF and CF bronchial epithelial cells. (ersjournals.com)
  • Interestingly, the expression of two IκB kinases (IKK)-α/β, the degradation of cytosolic IκB-β inhibitor and the NF-κB deoxyribonucleic acid binding activity were markedly reduced after FP treatment in both CF and non-CF bronchial epithelial cells. (ersjournals.com)
  • It was shown by the authors that fluticasone propionate exerts an anti-inflammatory effect by blocking a signal transduction leading to a reduced level of IκB-α/β kinases in bronchial epithelial cells. (ersjournals.com)
  • In particular the strong effect on the IκB-β kinase, which is known to be elevated in bronchial epithelial cells in cystic fibrosis patients, was observed. (ersjournals.com)
  • Cystic fibrosis (CF) is a lethal inherited disorder caused by mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) in airway epithelial cells 1 . (ersjournals.com)
  • One of the major clinical manifestations of CFTR mutations is neutrophil dominated inflammation 2 , 3 , implying that defective CFTR affects the immune function of airway epithelial cells, in particular the production of nuclear factor (NF)-κB-dependent chemokines. (ersjournals.com)
  • Increasing evidence suggests that respiratory epithelial cells with dysfunctional CFTR have exaggerated activation of NF-κB/inhibitor of NF-κB complex associated with upregulated expression of chemokines 4 , 5 . (ersjournals.com)
  • The present authors and previous studies, have previously demonstrated that epithelial expression of interleukin (IL)-8 is excessively elevated in the bronchial epithelial cells of human CF adults 6 . (ersjournals.com)
  • The effects of corticosteroids are predominantly mediated by the inhibition of NF-κB deoxyribonucleic acid (DNA)-binding activity, which controls genes encoding inflammatory cytokines, chemokines and adhesion molecules in respiratory epithelial cells 10 - 13 . (ersjournals.com)
  • In this study, cell toxicity and induction of reactive oxygen species (ROS) by Cr (Ⅵ) (1, 2, 4, 8 ㎛) in cultured human bronchial epithelial cells were investigated. (eaht.org)
  • ENaC is located in the apical membrane of polarized epithelial cells in particular in the kidney (primarily in the collecting tubule), the lung, the skin, the male and female reproductive tracts and the colon. (wikipedia.org)
  • Particulate matter (PM2.5) from biomass combustion induces an anti-oxidative response and cancer drug resistance in human bronchial epithelial BEAS-2B cells. (leibniz-hki.de)
  • Merk R, Heßelbach K, Osipova A, Popadić D, Schmidt-Heck W, Kim GJ, Günther S, Piñeres A G, Merfort I, Humar M (2020) Particulate matter (PM2.5) from biomass combustion induces an anti-oxidative response and cancer drug resistance in human bronchial epithelial BEAS-2B cells. (leibniz-hki.de)
  • Here we demonstrate that long-term exposure to fine particulate matter (PM 2.5 ) from biomass combustion had no impact on cellular viability and proliferation but increased intracellular reactive oxygen species (ROS) levels in bronchial epithelial BEAS-2B cells. (leibniz-hki.de)
  • Our study indicates that exosomal miR-221 derived from HQ-transformed malignant human bronchial epithelial cells is involved in the proliferation of recipient cells. (elsevierpure.com)
  • MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells. (edu.au)
  • Presented at the American Association for Cancer Research annual meeting on 6 April in San Diego, California, the research looked at human bronchial cells that contained some mutations found in smokers at risk of lung cancer. (nature.com)
  • 2. The aim of this work was to investigate how hypoxia affects human lung epithelial cells in combination with profibrotic stimuli and its correlation to pathogenesis. (lu.se)
  • Discussion: In conclusion, our data indicate that bronchial and alveolar epithelial cells respond differently to hypoxia and profibrotic stimuli. (lu.se)
  • Selective expression of RT6 superfamily in human bronchial epithelial cells. (nih.gov)
  • Here we show that SWCNTs dose and time-dependently caused toxicity in cultured human bronchial epithelial (BEAS-2B), alveolar epithelial (A549), and lung fibroblast (WI38) cells. (cdc.gov)
  • We determined the effect of vapour on oxidative stress-induced, PAFR-dependent pneumococcal adhesion to airway epithelial cells in vitro , and on pneumococcal colonisation in the mouse nasopharynx. (ersjournals.com)
  • Since invasive pneumococcal strains adhere to, and translocate across, respiratory tract epithelial cells, adhesion is a prerequisite for establishing pneumococcal disease [ 4 ]. (ersjournals.com)
  • Cilia are antennae-like protuberances that are present on 50-75% of the cells that line human airways. (newswise.com)
  • It is the first to look at the impact of flavoring chemicals in human epithelial cells, which are the type that line the lungs. (newswise.com)
  • In the new study, researchers used novel lab techniques that allowed them to examine the impact of both diacetyl and 2,3-pentanedione on epithelial cells in a system that closely mimicked the human airway epithelium in vivo. (newswise.com)
  • They exposed normal human bronchial epithelial (NHBE) cells to the chemicals for 24 hours. (newswise.com)
  • Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
  • Lung epithelial cells form a barrier that lines airway and alveolar surfaces and constitute an air-liquid interface. (springer.com)
  • Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. (novusbio.com)
  • Laboratory studies suggest that ginger can protect brain cells from the plaques that cause Alzheimer's disease, but this effect has not been studied in humans. (mskcc.org)
  • SCLC is a neuroendocrine tumor derived from bronchial mucosal epithelial argentophilic cells. (hindawi.com)
  • We analysed transcriptome of SARS-CoV-2 infected human lung epithelial cells, compared it with mock-infected cells, used network-based reporter metabolite approach and integrated the transcriptome data with protein-protein interaction network to elucidate the early cellular response. (tubitak.gov.tr)
  • Here, we present a new study on induction of autophagy in human lung epithelial cells by the APD HO53. (researchgate.net)
  • Epithelial cells are likely to modulate inflammation and tissue repair in the airways, but the factors responsible for these processes remain unclear. (epa.gov)
  • NRG1α was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
  • Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
  • 16-membered) on MUC5AC and MUC2 gene expression and secretion from human airway epithelial cells. (karger.com)
  • Confluent NCI-H292 human mucoepidermoid airways epithelial cells were pretreated with AZM or MDM for 2 h and then stimulated with 200 nmol/l phorbol 12-myristate 13-acetate (PMA) for 8 h. (karger.com)
  • The present study demonstrates that AZM and MDM suppress the synthesis of mucin and TNF-α from human airway epithelial cells. (karger.com)
  • Kim YD, Cho JS, Kim HS, Song SH: Effect of macrolide on the interleukin-1 β-mediated MUC2/5AC genes expression and mucin secretion in human airway epithelial cells. (karger.com)
  • Shimizu T, Shimizu S: Azithromycin inhibits mucus hypersecretion from airway epithelial cells. (karger.com)
  • Cigana C, Assael BM, Melotti P: Azithromycin selectively reduces tumor necrosis factor alpha levels in cystic fibrosis airway epithelial cells. (karger.com)
  • This has also been reported to work well in bronchial epithelial cells (UBH16E) and THP-1 cells. (novusbio.com)
  • TLR2 is differentially expressed in human cells. (novusbio.com)
  • For in vitro studies, human bronchial epithelial cells (Beas2b) were treated with waterpipe smoke extract (WPSE, 5%), nicotine (5mM), and/or cysteamine (250muM, an autophagy inducer and anti-oxidant drug), for 6hrs. (who.int)
  • Etiology Human papillomavirus (HPV) infects epithelial cells. (msdmanuals.com)
  • Carcinoid tumors arise from neuroendocrine cells, which are widespread in the human body, especially in the organs derived from the primitive intestine (see the image below). (medscape.com)
  • Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research Journal of Allergy. (nottingham.ac.uk)
  • it is also not known whether the flavoring chemicals similarly damage human airway epithelium. (nature.com)
  • The bronchial epithelium appears more responsive to changes in oxygen levels and remodelling processes compared to the alveoli, suggesting that hypoxia may be a driver of pathogenesis in chronic lung disorders. (lu.se)
  • Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
  • In the present study, it was investigated whether LMW-HA synthesised in the lung, in response to cyclic stretch, increased IL-8 production in the bronchial epithelium. (bvsalud.org)
  • We further show that quercetin, a dietary compound having preventive properties for lung cancer, decreased BPDE-stimulated IL-6 secretion from human lung fibroblasts through inhibition of the NF-κB and ERK pathways. (psu.edu)
  • Although SWCNTs are potentially suitable for a range of novel applications, their extremely small size, fiber-like shape, large surface area, and unique surface chemistry raise potential hazard to humans, including lung toxicity and fibrosis. (cdc.gov)
  • These results indicate that SWCNTs has a potential to induce human lung damage and fibrosis by damaging mitochondria, generating ROS, and stimulating production of proinflammatory and profibrogenic cytokines and growth factors. (cdc.gov)
  • During embryonic stages of lung organogenesis, TGF-β1 is pivotal in epithelial-mesenchymal interaction, during alveolarization and for airway branching morphology. (springer.com)
  • It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
  • Currently, lung carcinoma accounts for 30% of all carcinoma deaths in the world and is one of the most harmful carcinomas to human beings. (hindawi.com)
  • Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
  • Molecular subtypes of small cell lung cancer: a synthesis of human and mouse model data. (cancerindex.org)
  • In addition, normal human lung fibroblasts (NHLF) were exposed to mechanical stretch, which mimics ventilator-induced lung inflation, to evaluate the collagen secretion in culture medium. (bvsalud.org)
  • METHODS: This question was approached by stimulating a transformed human bronchial epithelial cell line with LMW-HA isolated from stretched human lung fibroblasts and probed for the activation of extracellular signal-regulated kinase pathways. (bvsalud.org)
  • Normal human bronchial epithelial (NHBE) cell cultures have been an effective model to assess the viral-host interaction. (aacc.org)
  • Here, we show that benzo[a]pyrene diol epoxide (BPDE, the active metabolite of cigarette smoke carcinogen benzo[a]pyrene)-induced human bronchial epithelial cell (HBEC) transformation was enhanced by IL-6 in vitro. (psu.edu)
  • Validation and application of human in vitro models for investigating bronchial response to cigarette smoke. (uni-muenchen.de)
  • They play a key role in keeping the human airway clear of mucus and dirt and allow people to breathe easily and without irritation. (newswise.com)
  • In addition to being implicated in diseases where fluid balance across epithelial membranes is perturbed, including pulmonary edema, cystic fibrosis, COPD and COVID-19, proteolyzed forms of ENaC function as the human salt taste receptor. (wikipedia.org)
  • Aroylated phenylenediamines (APDs) are novel modulators of innate immunity with respect to enhancing the expression of antimicrobial peptides and maintaining epithelial barrier integrity. (researchgate.net)
  • Fine particulate matter (PM 2.5 ) is the particulate matter with diameter equal to or less than 2.5 μm and has become a serious threat to human health as a number of epidemiological studies have demonstrated marked association between PM 2.5 exposure and increased incidence and aggravation of respiratory and cardiovascular diseases [ 1 , 2 ]. (medsci.org)
  • Public health officials and others concerned with appropriate actions to take at hazardous waste sites may want information on levels of exposure associated with more subtle effects in humans or animals (LOAEL) or exposure levels below which no adverse effects (NOAELs) have been observed. (cdc.gov)
  • Estimates of exposure levels posing minimal risk to humans (Minimal Risk Levels or MRLs) have been made for titanium tetrachloride. (cdc.gov)
  • An MRL is defined as an estimate of daily human exposure to a substance that is likely to be without an appreciable risk of adverse effects (noncarcinogenic) over a specified duration of exposure. (cdc.gov)
  • As these kinds of health effects data become available and methods to assess levels of significant human exposure improve, these MRLs will be revised. (cdc.gov)
  • Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
  • Using a small radiolabeled molecule, diethylene triamine pentaacetic acid (DTPA), we are able to quantify airway surface liquid (ASL) volume changes in both CF and non-CF human bronchial epithelial cell cultures. (confex.com)
  • Human infection with a novel avian-origin influenza A (H7N9) virus. (aacc.org)
  • So, there was a human infection back in 2002 in Virginia, and then another human case that was detected in New York in a person that visited these live poultry markets. (cdc.gov)
  • Chronic bronchitis is due to recurrent inflammation that may be associated with active infection resulting in degenerative changes within the bronchial tubes. (medscape.com)
  • The authors therefore investigated the response of an SV-40/adenovirus-transformed human bronchial epithelial cell line (BEAS-2B) to histamine, a mediator with relevance for airway diseases. (epa.gov)
  • Comparison of the data with published reports for human airway epithelia in primary culture suggests that the BEAS cell line is, in a number of respects, a relevant model for the study of airway epithelial responses to a variety of stimuli. (epa.gov)
  • Although the first epidemic has subsided, the presence of a natural reservoir and the disease severity highlight the need to evaluate its risk for human public health and to understand the possible pathogenesis mechanism. (aacc.org)
  • Response of a Human Bronchial Epithelial Cell Line to Histamine: Intracellular Calcium Changes and Extracellular Release of Inflammatory Mediators. (epa.gov)
  • This occurs because of the inflammatory response of the mucous membranes within the lungs' bronchial passages. (medscape.com)
  • Epithelial sodium channels facilitate Na⁺ reabsorption across the apical membranes of epithelia in the distal nephron, respiratory and reproductive tracts and exocrine glands. (wikipedia.org)
  • Notable human health toxicity effects identified from human and/or animal studies include respiratory cancer, non-cancer toxicity effects following inhalation, dermatitis, and reproductive effects. (mdpi.com)
  • Differences in human toxicity potencies/potentials of different nickel chemical forms are correlated with the bioavailability of the Ni 2+ ion at target sites. (mdpi.com)
  • We conclude that the observed effects contribute to loss of epithelial integrity during atopic asthma. (springer.com)
  • Asthma is a clinical syndrome characterized by episodic reversible airway obstruction, increased bronchial reactivity, and airway inflammation. (medscape.com)
  • Epidemiologic data demonstrate that particularly long-term emissions adversely affect human health. (leibniz-hki.de)
  • The development of the human blood-CSF-brain barrier. (cdc.gov)
  • Within the influenza division, we have a branch, and it's focused on surveillance for all influenza viruses, and that includes both human seasonal strains, as well as influenza viruses that circulate in animal hosts. (cdc.gov)
  • And I'm the team lead of a laboratory that focuses only on the zoonotic influenza viruses, or those strains that are typically found only in animals, but occasionally can infect humans. (cdc.gov)
  • Most of the time those influenza viruses don't infect people, but when they do, we're especially concerned because humans don't have immunity to the viruses that typically circulate in those other animals that I mentioned. (cdc.gov)
  • Cincinnati, OH: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health. (cdc.gov)
  • Infl uenza virus RNA human virus isolates of subtypes H1N1 (A/HongKong/ has been detected in feces, but its role is unknown ( 4 - 7 ). (cdc.gov)
  • This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)
  • Harnessing primary human bronchial epithelial cell culture for studies of airway epithelial injury and regeneration. (uni-muenchen.de)
  • Recently, the development of the HepG2-NTCP sec+ cell line, expressing the sodium taurocholate co-transporting polypeptide receptor (NTCP) and self-assembling co-cultured primary human hepatocytes (SACC-PHHs) has opened new perspectives for a better understanding of HBV and HDV lifecycles and the development of specific antiviral drug targets against HBV/HDV infections. (cdc.gov)
  • We know that there is a lot of different infectious diseases that are caused by viruses, parasites, bacteria that are typically found in animal hosts, but when humans get infected, they can cause disease, and influenza's no exception. (cdc.gov)
  • There was no difference in baseline nasal epithelial PAFR expression between vapers (n=11) and controls (n=6). (ersjournals.com)
  • Metabolic activity was significantly increased by hypoxia in both epithelial cell types. (lu.se)
  • The epithelial sodium channels are structurally and probably evolutionary related to P2X purinoreceptors, pain receptors that activate when they detect ATP. (wikipedia.org)
  • Protoelyzed variants of ENaC also function as human salt taste receptors. (wikipedia.org)
  • A Lectin histochemical analysis and double immuno- lthough infl uenza predominantly causes respiratory fl uorescence staining were used to study the distribution diseases, gastrointestinal signs such as diarrhea of infl uenza virus receptors on human small and large and vomiting are not uncommonly reported, particularly intestinal tissues. (cdc.gov)
  • Production and use of nickel and its compounds can, however, result in additional exposures to humans and the environment. (mdpi.com)
  • Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes. (nih.gov)
  • Title : Effect of Elevated Carbon Dioxide on Bronchial Epithelial Innate Immune Receptor Response to Organic Dust from Swine Confinement Barns Personal Author(s) : Schneberger, D.;Cloonan, D.;DeVasure, J. M.;Bailey, K. L.;Romberger, D. J.;Wyatt, T. A. (cdc.gov)
  • Multi-omics biomarkers of response to the discovered drugs were identified using human breast cancer cell lines. (cdc.gov)
  • This role was first confirmed using human sensory studies to evaluate the effect of 4-propylphenyl 2-furoate on the perception of the salty taste of table salt, sodium chloride (NaCl). (wikipedia.org)