• The idea of myocardial regeneration with stem cell transplantation after myocardial infarction receives tremendous interest. (escardio.org)
  • Several preliminary reports have demonstrated that local stem cell transplantation in patients with acute myocardial infarction is safe and may lead to improved myocardial function and perfusion. (escardio.org)
  • In experimental and clinical studies unselected mononuclear bone marrow cells as well as specific subpopulations have been used for transplantation. (escardio.org)
  • Early transplantation of bone marrow mononuclear cells promotes neuroprotection and modulation of inflammation after status epilepticus in mice by paracrine mechanisms. (fiocruz.br)
  • The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
  • Three patients with severe chronic lower limb ischemia accompanied by Burger's disease or collagen arteritis were enrolled in a pilot clinical trial of the novel cell therapy by transplantation of ex-vivo expanded immature erythroid cells. (bvsalud.org)
  • Stem-cell transplantation into the frontal motor cortex in amyotrophic lateral sclerosis patients. (cellsurgicalnetwork.com)
  • RESULTS: The autologous transplantation of CD133(+) stem cells into the frontal motor cortex is a safe and well-tolerated procedure in ALS patients. (cellsurgicalnetwork.com)
  • CONCLUSIONS: Stem-cell transplantation in the motor cortex delays ALS progression and improves quality of life. (cellsurgicalnetwork.com)
  • Pilot studies suggest that intracoronary transplantation of progenitor cells derived from bone marrow (BMC) or circulating blood (CPC) may improve left ventricular function after acute myocardial infarction. (pharmaceuticalintelligence.com)
  • The effects of cell transplantation in patients with healed myocardial infarction are unknown. (pharmaceuticalintelligence.com)
  • Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
  • Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
  • Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
  • The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
  • Regulatory T cells (T(regs)) manipulated ex vivo have potential as cellular therapeutics in autoimmunity and transplantation. (lu.se)
  • Although it is possible to expand naturally occurring T(regs), an attractive alternative possibility, particularly suited to solid organ and bone marrow transplantation, is the stimulation of total T cell populations with defined allogeneic antigen-presenting cells (APCs) under conditions that lead to the generation or expansion of donor-reactive, adaptive T(regs). (lu.se)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases, including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses. (medscape.com)
  • In this review we recorded several representative in vitro and in vivo studies performed in culture cell lines, in animal or clinical trials, indicating that the regular intake of EVOO is associated with enhanced neuroprotective, antioxidant, anti-inflammatory, anti-atherosclerotic, anti-cancer and anti-microbial properties. (researchgate.net)
  • Subsequent studies described a VEGFR2 and AC133 expressing subpopulation of these CD34 positive circulating cells that could form endothelial colonies in vitro [ 14 , 15 ]. (hindawi.com)
  • The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1). (nih.gov)
  • Specifically, the study focused on Kupffer cell proinflammatory cytokines IL-6 and TNF-α and activation of NF-κB after chronic ethanol feeding followed by in vitro stimulation with lipopolysaccharide (LPS). (uni-frankfurt.de)
  • In the paper titled 'Characterization of Bone Marrow Mononuclear Cells on Biomaterials for Bone Tissue Engineering In Vitro,' there was an error in the Acknowledgments. (uni-frankfurt.de)
  • The effect of IL-15 stimulation on IL-17 production by BM mononuclear cells was examined in vitro. (biomedcentral.com)
  • In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
  • Perfusion cultures of human bone marrow mononuclear cells (BMMNC) provide a unique in vitro model of hematopoiesis, supporting growth of both accessory and hematopoietic elements. (ucsd.edu)
  • There is evidence that 2D or 3D culturing conditions have an impact on the cells' secretome, and here we aimed to compare the secretion of cytokines and growth factors in human MSCs from different sources cultured in 2D and 3D conditions and assess their effect on human macrophages polarization in vitro. (biomedcentral.com)
  • The initial bone marrow blast percentage correlated well with the in vitro growth number. (docksci.com)
  • Beside bone m a r r o w morphology and k a r y o t y p e [4, 5, 6], bone m a r r o w cultures have attracted great interest [7-10], since the disturbed haematopoiesis in M D S patients is reflected in their in vitro bone m a r r o w growth patterns. (docksci.com)
  • Previous studies have proven that rodent and human MSCs can differentiate into HLCs in vitro and in vivo , which is one of the mechanisms of MSCs in the treatment of liver failure. (springer.com)
  • More recently, ontogenetic, transcriptional, and proteomic research approaches uncovered major developmental differences between Flt3L-dependent conventional DCs as compared with Mphs and monocyte-derived DCs (MoDCs), the latter mainly generated in vitro from murine bone marrow-derived DCs (BM-DCs) or human CD14\(^{+}\) peripheral blood monocytes. (uni-wuerzburg.de)
  • Conversely, in vitro GM-CSF-dependent monocyte-derived Mphs largely resemble MoDCs whereas tissue-resident Mphs show a common embryonic origin from yolk sac and fetal liver with Langerhans cells (LCs). (uni-wuerzburg.de)
  • Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
  • These cells inhibited T cell proliferation in a standard in vitro mixed lymphocyte assay and, moreover, attenuated the development of vasculopathy mediated by autologous peripheral blood mononuclear cells in a functionally relevant humanized mouse transplant model. (lu.se)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
  • This study tested the hypothesis that double overexpression of miR-19a & miR-20a (dOex-mIRs) in human induced pluripotent stem cell (iPS)-derived mesenchymal stem cells (MSCs) effectively preserved left-ventricular-ejection-fraction (LVEF) in dilated cardiomyopathy (DCM) (i.e., induced by doxorubicin) rat. (researchsquare.com)
  • Bone marrow mononuclear cells (BMMNCs) consist of hemopoietic stem cells and mesenchymal stem cells together with progenitors of endothelial cells or somatic cells [ 1 , 2 ]. (biomedcentral.com)
  • Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
  • In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
  • mesenchymal stem cells are most commonly used because they are easy to obtain and present no ethical problems. (springer.com)
  • Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)
  • The bone morrow contains several stem cell types including hematopoetic stem cells, endothelial progenitor cells, mesenchymal (stromal) stem cells and multipotent adult progenitor cells. (escardio.org)
  • Although there is no definitive evidence, combinations of progenitor cells seem to be more benefical than specific stem cell type for cardiac repair (3). (escardio.org)
  • Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)
  • Vasculogenesis is the coalescence of new blood vessels from individual endothelial cells or progenitor cells. (hindawi.com)
  • These cells were therefore described as bone marrow derived endothelial progenitor cells (EPCs). (hindawi.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • These secreted factors may stimulate vascular growth and redesigning, attenuate fibrosis, modulate swelling, regulate cell differentiation and survival, and sponsor resident come or progenitor cells.12,13 Activation of these pathways may blunt reperfusion injury or attenuate adverse remodeling in individuals suffering from acute myocardial infarction (AMI) or HF, respectively. (bioinf.org)
  • Type I collagen, fibrin and PuraMatrix matrices provide permissive environments for human endothelial and mesenchymal progenitor cells to form neovascular networks. (lonza.com)
  • In this study, the author investigated the capability of human endothelial progenitor cells (EPCs) and mesenchymal progenitor cells (MPCs) to form microvascular networks on various substrates and in immune-deficient mice. (lonza.com)
  • We tested whether human endothelial progenitor cells (EPCs) and mesenchymal progenitor cells (MPCs) could form microvascular networks in type I collagen, fibrin and an engineered peptide hydrogel, PuraMatrix, in 7 days in vivo in immune-deficient mice. (lonza.com)
  • Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
  • Mycobacterium tuberculosis causes chronic infection of mononuclear phagocytes, especially resident (alveolar) macrophages, recruited macrophages, and dendritic cells. (escholarship.org)
  • We used a combination of congenic monocyte adoptive transfer, and pulse-chase labeling of DNA, to determine the kinetics and characteristics of trafficking, differentiation, and infection of mononuclear phagocytes during the chronic, adaptive immune phase of M. tuberculosis infection in mice. (escholarship.org)
  • Mononuclear phagocytes: tissue distribution and functional heterogeneity. (ox.ac.uk)
  • In the liver, KASV replicated in the cytoplasm of hepatocytes, to a lesser extent in mononuclear phagocytes, and rarely in presumptive endothelial cells. (cdc.gov)
  • These landmark publications have been the basis for the new field of autologous bone marrow stem cell therapy for heart disease. (wikipedia.org)
  • Although tumor cells were first thought to drive the cellular events underpinning tumor angiogenesis and growth, considerable evidence has now emerged for the central role of tumor infiltrating myeloid cells such as monocytes, macrophages, and neutrophils in this phenomenon [ 8 - 12 ]. (hindawi.com)
  • We found that Ly6Chi monocytes traffic rapidly to the lungs, where a subpopulation become Ly6Clo and remain in the lung vascular space, while the remainder migrate into the lung parenchyma and differentiate into Ly6Chi dendritic cells, CD11b+ dendritic cells, and recruited macrophages. (escholarship.org)
  • in contrast, cells that differentiate from Ly6Chi monocytes turn over more slowly, but diminish in frequency in less than one week. (escholarship.org)
  • Our findings that mononuclear phagocyte populations at the site of M. tuberculosis infection are highly dynamic provide support for specific approaches for host-directed therapies directed at monocytes, including trained immunity, as potential interventions in TB, by replacing cells with limited antimycobacterial capabilities with newly-recruited cells better able to restrict and kill M. tuberculosis. (escholarship.org)
  • These cells are closely related to neutrophils and monocytes. (aacrjournals.org)
  • Mononuclear myeloid cells include terminally differentiated macrophages and dendritic cells (DC), as well as monocytes, which under inflammatory conditions differentiate in tissues to macrophages and DCs. (aacrjournals.org)
  • Myeloid cells generated under these conditions, although similar to neutrophils and monocytes in morphology and phenotype, have different genomic and biochemical profiles and functional activity. (aacrjournals.org)
  • The field started changing in the late 1990s when the Gr1 + CD11b + cellular phenotype was suggested as defining the immune-suppressive myeloid cells in spleens of mice and when these cells were shown to be phenotypically similar but functionally distinct from monocytes and neutrophils ( 2, 3 ). (aacrjournals.org)
  • Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than the erythrocytes and the polymorphonuclear (PMN) leukocytes (granulocytes). (aralbio.com)
  • Human peripheral blood mononuclear cells-derived macrophages were then treated with umbilical cord-derived MSCs' conditioned media and their effect on macrophages polarization was assessed. (biomedcentral.com)
  • Peripheral blood mononuclear cells were obtained after admission by leukapheresis. (cellsurgicalnetwork.com)
  • In this study production levels of interleukins (IL)-12 and IL-13 were measured by commercial ELISA in culture supernatants of mitogen-stimulated peripheral blood mononuclear cells from 30 non-splenectomized beta-thalassaemia cases with iron overload and 20 age- and sex-matched healthy individuals. (who.int)
  • Adult-male SD rats (n=32) were equally divided into groups 1 (sham-operated control), 2 (DCM), 3 (DCM + iPS-MSCs/1.2 x 10 6 cells/administered by post-28 day's DCM induction) and 4 (DCM + iPS-MSC dOex-mIRs /1.2 x 10 6 cells) and euthanized by day 60 after DCM induction. (researchsquare.com)
  • Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
  • In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
  • Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
  • Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
  • There is a growing body of evidence that multipotent mesenchymal stromal cells' (MSCs') remarkable therapeutic potential is attributed not only to their differentiation and regenerative capacity, but also to the paracrine effect, underlying their immunomodulatory properties. (biomedcentral.com)
  • Umbilical cord-derived MSCs' conditioned media hold great potential for therapeutic use, demonstrating significant anti-inflammatory effect on human macrophages. (biomedcentral.com)
  • Multipotent mesenchymal stromal cells (MSCs) are an extensively researched yet still understudied tool for treating a wide range of pathological conditions, one particular area of research interest being their immunomodulatory properties. (biomedcentral.com)
  • MSCs are reported to contribute both to the inflammation initiation and to its resolution via complex cross talk with immune cells, most importantly macrophages [ 1 ]. (biomedcentral.com)
  • MSCs have been shown to suppress both the transition of human macrophages from the M0 to M1 state and the pro-inflammatory activity of macrophages already polarized into the M1 state [ 4 ]. (biomedcentral.com)
  • Thus, bone marrow is the first established and still one of the most commonly used sources of MSCs [ 7 ]. (biomedcentral.com)
  • however, relatively low cell yield and high invasiveness of the harvesting procedure [ 9 ] have urged the quest for alternative MSCs sources. (biomedcentral.com)
  • Despite this imperfect knowledge, sufficient medical encounter offers been accumulated from the several medical tests using numerous adult come cell populations, including bone tissue marrow-derived mononuclear cells (BMMNCs), mesenchymal come cells (MSCs), and come cells separated from cardiac cells (Number 1). (bioinf.org)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
  • To verify this hypothesis, we examined the frequency of Th1, Th2 and Th17 cell populations as well as the concentrations of cytokines involved in Th17 cell differentiation and migration in BM from RA and OA patients. (biomedcentral.com)
  • To address this question we first developed human oligonucleotide microarrays with 30.000 elements and then performed large-scale expression profiling of long-term expanded MSC and MSC during differentiation into osteoblasts. (biomedcentral.com)
  • Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
  • We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
  • Epidermal appendages are intradermal epithelial structures lined with epithelial cells with the potential for division and differentiation. (medscape.com)
  • It is involved in cell differentiation, maintenance of cell differentiation state, tumorigenesis, and cell senescence, mainly through DNA methylation, histone acetylation, and RNA-related silencing [ 6 ]. (hindawi.com)
  • Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
  • Repair of scar tissue constitutes a challenge for cardiac stem cell therapy due to lack of adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
  • In order to characterize more fully and assess its possible functions, 15 samples of surgical and autopsy human thyroid tissue were studied by classical histology, immunohistochemistry, transmission electron microscopy, electron microscopic immunohistochemistry, and scanning electron microscopy. (nih.gov)
  • The main constituent of the human thyroid stromal tissue was a CD34 positive reticular network involving fibroblasts, mononuclear cells and nerve terminals. (nih.gov)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Bone marrow mononuclear cells (BMCs) are suitable for bone tissue engineering. (uni-frankfurt.de)
  • Ly6C high MC is the pro-inflammatory subset and the counterpart of human CD14 ++ CD16 + intermediate MC which contributes to systemic and tissue inflammation in various metabolic disorders, including hyperhomocysteinemia (HHcy). (frontiersin.org)
  • In the course of RA, the BM microenvironment can promote the development of Th17 cell responses and overproduction of IL-17AF that may lead to increased inflammation and tissue destruction in RA BM. (biomedcentral.com)
  • One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
  • Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
  • Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
  • Despite improved surgical techniques, the tendon-to-bone healing rate is unsatisfactory due to difficulties in restoring the delicate transitional tissue between bone and tendon. (wjgnet.com)
  • This led to the first human applications using cryopreserved aortic allografts that present key advantages because they are available in tissue banks and do not require immunosuppressive therapy. (biomedcentral.com)
  • At the time Grillo wrote his reference textbook, tracheal replacement using the tissue-engineered technique had not yet been applied to malignant tumors, as it required the use of the patient's own cells and because several months were required to construct the graft. (biomedcentral.com)
  • eg, bone, bone marrow, and skin grafts) Genetically identical (syngeneic [between monozygotic twins]) donor tissue (isografts) Genetically. (msdmanuals.com)
  • The innate immune system is mainly composed of myeloid lineage cells, such as macrophages, neutrophils, and mast cells [ 2 ]. (hindawi.com)
  • Dendritic cells (DCs) and macrophages (Mph) share many characteristics as components of the innate immune system. (uni-wuerzburg.de)
  • More recently, ontogenetic, transcriptional, and proteomic research approaches uncovered major developmental differences between Flt3L-dependent conventional DCs as compared with Mphs and monocyte-derived DCs (MoDCs), the latter mainly generated in Dendritic cells (DCs) and macrophages (Mph) share many characteristics as components of the innate immune system. (uni-wuerzburg.de)
  • Prepare specimen mixtures in separate 15-mL tube: Mix 5 mL of blood/bone marrow aspirate with 5 mL of PBS. (mdanderson.org)
  • BMCs were purified from human bone marrow aspirate samples. (uni-frankfurt.de)
  • Human MSC are easy to isolate from small aspirate of bone marrow via their adherence ability. (biomedcentral.com)
  • Further enrollment of patients with chronic severe lower limb ischemia is necessary to confirm the efficacy and safety of this novel cell therapy, and to estimate the necessary amount of bone marrow aspirate. (bvsalud.org)
  • The criteria to classify the multitude of subsets within the mononuclear phagocyte system are currently phenotype, ontogeny, transcription patterns, epigenetic adaptations, and function. (uni-wuerzburg.de)
  • In this study, formalin-fixed paraffin-embedded tissues from a previous experiment in which KASV infection was confirmed in 18 experimentally infected ERBs were used for an in-depth analysis using histopathology, in situ hybridization (ISH) for detection of viral RNA, immunohistochemistry (IHC) to assess the mononuclear phagocyte system response, and quantitative digital image analysis to investigate virus clearance from the liver and spleen within a spatial context. (cdc.gov)
  • Revision accepted 3 September 1989) Abstract--Bone marrow cultures and survival time were studied in 39 patients with primary myelodysplastic syndromes. (docksci.com)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • It is now known that new blood vessels originate from preexisting vessels by activation, proliferation and migration of endothelial cells through a process named "angiogenesis" [ 4 ]. (hindawi.com)
  • Specific growth factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), stimulate the proliferation and migration of naturally quiescent endothelial cells, resulting in the formation of new vessel structures during embryonic development and tumor growth [ 7 ]. (hindawi.com)
  • This suggested that EPCs are able to differentiate into endothelial cells and that such cells are incorporated into sites of active angiogenesis including ischemia, tumor angiogenesis, and metastasis in adult organisms [ 16 , 17 ]. (hindawi.com)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Granulocytic myeloid cells include populations of terminally differentiated polymorphonuclear neutrophils, eosinophils, basophils, and mast cells. (aacrjournals.org)
  • Suspension culture of human mast cells/basophils from umbilical cord blood mononuclear cells. (virascience.com)
  • Objective(s) Human umbilical cord blood (HUCB) is now considered as a valuable source for stem cell-based therapies. (virascience.com)
  • Here we demonstrate that stimulation of mouse CD4(+) T cells by immature allogeneic dendritic cells combined with pharmacological inhibition of phosphodiesterase 3 (PDE) resulted in a functional enrichment of Foxp3(+) T cells. (lu.se)
  • Preclinical and some small-scaled clinical trials have suggested feasibility and safety of cardiac stem cell therapy. (escardio.org)
  • Cell therapy is currently emerging as a potential new treatment for post MI patients with the assumption that recolonization of the areas of scarred myocardium with exogenously supplied surrogates or precursors of cardiomyocytes can restore function and ultimately affect clinical outcomes. (escardio.org)
  • Various cell types have been tested experimentally for cardiac repair so far, but only those of autologous origin have yet undergone clinical testing due to immune competency. (escardio.org)
  • However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. (biomedcentral.com)
  • Effective medical translation, however, remains demanding due to inconclusive study results concerning come cell regenerative capacity and their ability to improve cardiac function.2C6 Here we will evaluate the proposed mechanisms of action for originate cell regenerative therapy, review various originate cell sources, and discuss the merits and limitations of recently published adult originate cell clinical tests. (bioinf.org)
  • Come Cells Utilized in Clinical Tests For Heart Diseases 329045-45-6 Although animal studies support the idea that the beneficial effects observed from treatment with adult come cells are attributable to paracrine effect,13,16,17, the precise mechanism of action in humans remains ambiguous. (bioinf.org)
  • Next, we used methylation-specific polymerase chain reaction (MSP) technology for detecting the methylation of GRHL2 in clinical samples with acute leukemia and cell lines. (hindawi.com)
  • More recently, few clinical attempts have offered encouraging results with ex vivo or stem cell-based engineered airways and tracheal allografts implanted after heterotopic revascularization. (biomedcentral.com)
  • Tickborne rickettsial diseases in humans often share similar clinical features yet are epidemiologically and etiologically distinct. (cdc.gov)
  • Initial management consisted of en una paciente con topical corticosteroids and oral antihistamines with little clinical response. (bvsalud.org)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. (nih.gov)
  • Our results showed that the treatment with A. cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. (nih.gov)
  • The results of our preliminary study demonstrated that 6-OAP inhibits the proliferation of human MM cells by inducing the arrest of mitosis and inhibiting specific key pathways ( 5 ). (spandidos-publications.com)
  • Finally, we studied the effects of arsenide trioxide and 5-azacitidine on the proliferation of leukemia cells and the TGF- β signaling pathway. (hindawi.com)
  • At the same time, arsenide trioxide and 5-azacitidine are associated with the inhibition of cellular proliferation of acute leukemia cells and also promote the elevated expression of TGF- β signaling pathway-linked proteins, including TGF- β , Smad2, Smad3, and Smad4. (hindawi.com)
  • Without further manipulation or selection, the resultant population delayed skin allograft rejection mediated by polyclonal CD4(+) effectors or donor-reactive CD8(+) T cell receptor transgenic T cells and inhibited both effector cell proliferation and T cell priming for interferon-γ production. (lu.se)
  • Ly6C low MC manifested activated T-cell signaling pathways and potentially can adapt the function of lymphocytes. (frontiersin.org)
  • Lymphoprep is specifically formulated to be used for the isolation and purification of lymphocytes, which are a type of white blood cell that plays an important role in the immune system. (aralbio.com)
  • subtypes of cutaneous T-cell lymphoma, SS is a malignant neoplasm originating the most common of which are mycosis from T lymphocytes, which involves the fungoides and Sézary syndrome (SS). (bvsalud.org)
  • They can skin and can extend to the bone marrow, simulate benign skin disorders, making them blood lymphocytes, lymph nodes, and a diagnostic challenge for dermatologists1,2. (bvsalud.org)
  • Tumors exhibit significant myeloid cell infiltrates, which are actively recruited to the tumor microenvironment. (hindawi.com)
  • Finally, we will comment on the mechanisms regulating myeloid cell recruitment to the tumor microenvironment and on the potential of myeloid cells as new targets for cancer therapy. (hindawi.com)
  • In the present study we hypothesized that the RA BM microenvironment supports the development of Th17 cells and Th17 cell response. (biomedcentral.com)
  • Effective immunotherapy of stromal-rich tumors requires simultaneous targeting of cancer cells and immunosuppressive elements of the microenvironment. (aacrjournals.org)
  • As such, stem cell therapy cannot be regarded as a valid therapeutic option for patients with cardiovascular disease in the present era of evidenced-based medicine, yet it holds great hope for the future. (escardio.org)
  • With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
  • Therefore, targeting these cells may represent an attractive therapeutic opportunity. (aacrjournals.org)
  • Oddly enough, recent studies possess demonstrated that these factors may become clustered into extracellular membrane vesicles, including exosomes and microsomes, which can then transfer proteins, lipids, RNA, and microRNAs to mediate cardioprotection.14,15 Although further studies are needed to confirm that these vesicles can substitute for originate cell therapy, delivering these vesicles rather than cells themselves may present a clinically attractive therapeutic option from a regulatory and commercial perspective. (bioinf.org)
  • The development of animal models of dengue virus (DENV) infection and disease has been challenging, as epidemic DENV does not naturally infect non-human species. (mdpi.com)
  • Despite the importance of these cells in tuberculosis (TB) pathogenesis and immunity, little is known about the population dynamics of these cells at the sites of infection. (escholarship.org)
  • New cells (identified by pulse-chase labeling) acquire bacteria within 1-3 days of appearance in the lungs, indicating that bacteria regularly encounter new cellular niches, even during the chronic stage of infection. (escholarship.org)
  • 13] The inflammation and necrosis observed during HAV infection does not appear to be a direct viral effect but rather an effect of the immune cell response induced by the viral infection. (medscape.com)
  • It is indicated for the treatment of human immunodeficiency virus-1 (HIV-1) infection in adults and adolescents weighing at least 35 kg. (who.int)
  • Cell therapy is a potential new treatment for post MI patients, but the main challenges are : the necessity to preserve immune competency and to gain adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
  • The human immune system is composed of an innate and an adaptive branch. (hindawi.com)
  • Importantly, during tumor progression, myeloid cells are implicated in promoting tumor angiogenesis, causing resistance against antiangiogenic therapies in cancer, and suppressing the immune response during cancer [ 3 - 5 ]. (hindawi.com)
  • Non-human primates (NHPs) can sustain viral replication in relevant cell types and develop a robust immune response, but they do not develop overt disease. (mdpi.com)
  • In addition, recent evidence indicates that IL-17 could also be produced by several innate immune cells and activated or inflammatory T cells [ 7 ]. (biomedcentral.com)
  • The main functional characteristic of these cells is their potent ability to suppress various types of immune responses. (aacrjournals.org)
  • Reports on the accumulation of immune-suppressive myeloid cells associated with tumor progression were published sporadically beginning in the early 1970s ( 1 ). (aacrjournals.org)
  • During the 1980s and early 1990s, work from the laboratories of Diana Lopez, Jim Talmadge, M. Rita Young, and Hans Schreiber demonstrated that various types of myeloid cells could inhibit immune function in cancer. (aacrjournals.org)
  • The observations of the accumulation in spleens and tumors of large numbers of these cells with potent immune-suppressive activity were readily reproducible in most murine tumor models. (aacrjournals.org)
  • Our team of expert immunologists have optimized protocols for accurate detection of immune cell subsets across sample types. (teiko.bio)
  • Resuspend the cells by gentle aspiration with a Pasteur pipette. (mdanderson.org)
  • Bone marrow aspiration was conducted from the patients' bilateral anterior iliac crest under general anesthesia in an operating theater. (biomedcentral.com)
  • Bone marrow aspiration was conducted from the patients' bilateral anterior iliac crest under general anesthesia in an operating theater at Vinmec Times City International Hospital. (biomedcentral.com)
  • Preparation of cells Bone marrow cells were obtained by aspiration into sterile tubes containing 2 ml Iscove's modified Dulbecco's medium (IMDM) (Gibco) with 200 IU preservative-free heparin. (docksci.com)
  • Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
  • Use this density gradient medium for rapid, simple, and reliable cell isolation from most blood samples obtained from normal individuals and patients. (aralbio.com)
  • A simple and effective method for the isolation of mononuclear cells from human blood was reported by Dr. Arne Bøyum in 1968. (aralbio.com)
  • We illustrated for the first time in a human model the three main stages of osteogenic development, and we could show the diverse regulation of the SMAD pathways by TGF-β2 and BMPs. (biomedcentral.com)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • Given the complexity of human transcriptional regulation in vivo and the multitude of potential epigenetic mechanisms cooperating to regulate transcriptional activity, questions remain about the interplay of regulatory mechanisms in patients with CMML. (nature.com)
  • In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
  • This system provides a useful model for studying relationships within GF networks and for elucidating the conditions that result in primitive cell expansion ex vivo. (ucsd.edu)
  • We then tried to establish a novel cell therapy by implantation of ex vivo expanded immature erythroblasts cultured from hematopoietic stem/precursor cells. (bvsalud.org)
  • The in vivo angiogenic effects of implantation of the ex vivo expanded cells were stronger than that of BMI in mouse limb ischemia model. (bvsalud.org)
  • These data establish a method for the ex vivo generation of graft-reactive, functional mouse and human T(regs) that uses a clinically approved agent, making pharmacological PDE inhibition a potential strategy for T(reg)-based therapies. (lu.se)
  • Myeloid cells promote tumor growth by stimulating tumor angiogenesis, suppressing tumor immunity, and promoting metastasis to distinct sites. (hindawi.com)
  • In this review, we discuss the role of myeloid cells in promoting tumor angiogenesis. (hindawi.com)
  • In 1971, Dr. Judah Folkman observed that neovascularization occurs around tumors and proposed that new blood vessel growth is necessary to supply nutrients and oxygen to tumor cells during exponential tumor growth [ 6 ]. (hindawi.com)
  • However, in cancer, myeloid cells are largely converted to serve a new master-tumor cells. (aacrjournals.org)
  • These cells have emerged as an important contributor to tumor progression. (aacrjournals.org)
  • Next, we developed targeted MS assays, and applied these to cell lines and primary patient samples to refine the list of candidate tumor markers. (bmj.com)
  • Bone marrow loss and a high proclivity to leukemic progression are the characteristics of myelodysplastic syndrome (MDS). (techscience.com)
  • More specifically, Epo consumption was correlated (r = 0.92 and 0.96) with the number of glycophorin A-positive (glyA+) cells produced, and the rate of Epo consumption varied with the progression of cells through the erythroid lineage. (ucsd.edu)
  • Preeminent among these proteins are the cyclin dependent kinases, which upon binding to cyclins, phosphorylate numerous targets to trigger cell cycle progression. (smadpathway.com)
  • Compared with follicular epithelial cells, there are a few morphological studies on the stroma of human thyroid gland. (nih.gov)
  • Diagnosis of MM is based on morphological features, imaging studies, analysis of myeloma cell-produced proteins, and testing of the blood, urine, and bone marrow (BM). (bmj.com)
  • The cells were cultured in RPMI-1640 medium supplemented with 10% (for U266) or 15% (for RPMI 8226 and MM.1S) fetal bovine serum (Hyclone Laboratories, Inc., Logan, UT, USA) and incubated in a humidified atmosphere with 5% CO 2 at 37°C. (spandidos-publications.com)
  • CFU-GM assay CFU-GM were cultured in a medium containing 20% fetal calf serum (FCS), 10% conditioned medium from the human bladder carcinoma cell line 5637 [12] or 10% human placental conditioned medium (HPCM) as a source of CSF [13], 0.3% agar and IMDM in a fully humidified atmosphere at 37°C and 5% CO 2. (docksci.com)
  • Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
  • Murine Ly6C high and Ly6C middle MC subsets perform pro-inflammatory functions, which are considered the counterpart of human CD14 ++ CD16 + intermediate MC or CD14 + CD40 + inflammatory MC ( 4 , 5 ). (frontiersin.org)
  • mononuclear cells (PBMCs). (cdc.gov)
  • It represents a highly ordered stroma, with potential structural and functional similarities to the stroma of bone marrow (Yamazaki and Allen, 1990). (nih.gov)
  • Kolman A, Chovanec M, Osterman-Golkar S. Genotoxic effects of ethylene oxide, propylene oxide and epichlorohydrin in humans Update review (1990-2001). (who.int)
  • Out of the 313 unique human cytokines evaluated, 11 were found to expand cell numbers ≥2-fold in a 7-day culture. (haematologica.org)
  • Cytokines are essential for the function and maintenance of cells, and altered cytokine levels influence not only leukemic cells, but also the normal HSC within the BM. (haematologica.org)
  • Growth factor consumption and production in perfusion cultures of human bone marrow correlate with specific cell production. (ucsd.edu)
  • Immunohistochemistry using a monoclonal antibody against Cx43 confirmed the distribution of gap junctions between stromal fibroblastic cells, which was compatible with the ultrastructural findings. (nih.gov)
  • While his early research was into hypertensive heart disease, his notable contributions were his novel approach to improving cardiac function by bone-marrow derived stem cell treatment involving injections into the coronary arteries with consistent large favorable effects. (wikipedia.org)
  • These serve an important role as the source of epithelial cells that are responsible for reepithelialization if overlying epidermis be removed or destroyed in certain situations, such as partial-thickness burns, chemical peeling, dermabrasion, or traumatic abrasions. (medscape.com)
  • Secondly, we employed qRT-PCR and Western blotting to detect the mRNA and protein levels of GRHL2 in leukemia cell lines. (hindawi.com)
  • Then we investigated the demethylating effect of arsenic trioxide and 5-azacitidine on the mRNA and protein expression levels of GRHL2 in cell lines of acute leukemia. (hindawi.com)
  • The methylation level of the GRHL2 promoter region in acute leukemia patients and cell lines was significantly higher than the normal control group, and we found the elevated mRNA and protein levels of GRHL2 in acute leukemia cell lines after the use of the demethylation drug arsenic trioxide and 5-azacitidine. (hindawi.com)
  • Immature to mature erythroblasts were purified from human bone marrow, and mRNA expression were analyzed. (bvsalud.org)
  • This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. (nih.gov)
  • Identification of human immunodeficiency virus type 1 envelope genes recombinant between subtypes B and F in two epidemiologically linked individuals from Brazil. (fiocruz.br)
  • Furthermore, we describe a subset of myeloid cells with immunosuppressive activity (known as myeloid-derived suppressor cells). (hindawi.com)
  • This process is epitomized by myeloid-derived suppressor cells (MDSC). (aacrjournals.org)
  • The Working Group was not aware of any good rodent models that simulate the occurrence of acute myeloid leukemia in humans. (who.int)
  • Is stem cell therapy ready for patients? (wikipedia.org)
  • Stem Cell Therapy for Cardiac Repair. (wikipedia.org)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • Importance Come cell therapy is a promising treatment strategy for individuals with heart failure, which accounts for over 10% of deaths in the U. marrow mononuclear cells, have found only a humble benefit in individuals receiving come cell therapy. (bioinf.org)
  • Findings and Relevance Although come cell therapy for cardiovascular disease is definitely not yet ready for routine medical software, significant progress continues to become made. (bioinf.org)
  • Proposed Mechanisms of Action to Improve Heart Function Over the last decade, investigators possess proposed three fundamental mechanisms to support the assertion that come cell therapy can become used as an effective treatment for HF (Number 1). (bioinf.org)
  • Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
  • Angiogenesis therapy by bone marrow-mononuclear cell implantation (BMI) has been utilized. (bvsalud.org)
  • Moreover this novel cell therapy required only a small amount of bone marrow collection. (bvsalud.org)
  • Chronic myeloid neoplasms are malignant clonal hematopoietic stem cell disorders driven by recurrent genetic events, with an inherent risk of transformation to acute myeloid leukemia (AML) 1 , 2 . (nature.com)
  • Myeloid cells are a heterogeneous population of bone marrow-derived cells that play a critical role during growth and metastasis of malignant tumors. (hindawi.com)
  • Background Multiple myeloma (MM) is characterized by clonal expansion of malignant plasma cells in the bone marrow. (bmj.com)
  • A dermatology work-up was initiated, and the síndrome de Sézary initial diagnosis of malignant T-cell neoplasm was confirmed. (bvsalud.org)
  • The highest density of MNCs in each ml of bone marrow was found in patients with CP due to OD, whereas the percentage of CD34+ cells was the highest among patients with CP related to NI. (biomedcentral.com)
  • Otherwise, the POI was not significantly correlated with the peripheral blood white blood cell (WBC) count, monocyte percentage (M%) or haemoglobin (HGB) content. (biomedcentral.com)
  • High-risk MDS cases had a significantly higher percentage of apoptotic CD34+ cells and anti-apoptotic survivin+ cells than controls, particularly for trisomy 8 cases. (who.int)
  • 15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
  • INTRODUCTION THE MYELODYSPLASTIC syndromes (MDS) are a group of haematological disorders characterized by dysplasia in the h a e m a t o p o i e t i c cell lines [1]. (docksci.com)