• Laminin-10/11 was highly adhesive to lineage-committed myelomonocytic and erythroid progenitor cells and most lymphoid and myeloid cell lines studied, whereas laminin-8 was less adhesive. (ox.ac.uk)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • Vasculogenesis is the coalescence of new blood vessels from individual endothelial cells or progenitor cells. (hindawi.com)
  • We demonstrated for the first time that gene silencing of endothelial E-selectin using siRNA transfection in human umbilical vein endothelial cells (HUVECs) causes inhibition of EPC adhesion under flow conditions. (scirp.org)
  • The pellet is the stromal vascular fraction containing endothelial cells, immune cells, blood cells and progenitor cells. (jove.com)
  • Additionally, our research approach proved to be a powerful tool in the identification of novel biomarkers for cardiovascular and metabolic disease (e.g. circulating endothelial cells and progenitor stem cells [EPCs] for better prognosis). (nymc.edu)
  • Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. (biolegend.com)
  • Peripheral blood monocytosis >1 x 109/L. Less than 20% blasts (including promonocytes) in the blood and bone marrow (blast count is less than 2% on average) Splenomegaly At least one of: Mutation in RAS or PTPN11 Diagnosis of neurofibromatosis 1 Chromosome 7 monosomy Or two or more of the following criteria: Hemoglobin F increased for age. (wikipedia.org)
  • Peripheral blood contamination in human bone marrow aspirates was calculated from the ratios between the erythrocyte and nucleated cell counts in both the bone marrow aspirate and a simultaneously obtained peripheral blood sample. (karger.com)
  • 13 ] isolated mononuclear cells from human peripheral blood that were enriched for expression of the hematopoietic stem cell marker CD34 [ 13 ]. (hindawi.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • It is also intended for use in testing pools of human plasma and pools of human serum composed of equal aliquots of not more than 16 individual specimens from donors of whole blood, blood components, hematopoietic stem/progenitor cells sourced from bone marrow, peripheral blood or cord blood, and from donors of donor lymphocytes for infusion. (fda.gov)
  • These include bone marrow-derived cells, peripheral blood derived stem cells and skeletal myoblasts (3). (escardio.org)
  • Chromosomal aberrations (hypo- and hyperdiploidy, deletions, breaks, and gaps) in peripheral lymphocytes and bone marrow cells are the predominant effects seen in humans. (cdc.gov)
  • This disorder is characterized by reduction of all cellular elements in the peripheral blood and in bone marrow, leading to fibrosis, an irreversible replacement of bone marrow. (cdc.gov)
  • 1 This includes human cells for transplantation such as haematopoietic stem cells from bone marrow, peripheral blood or cord blood. (who.int)
  • Aplastic anemia is a syndrome of bone marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. (medscape.com)
  • Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)
  • Transplantation of ALDHhl mixed progenitor cells or culture- expanded multipotent stromal cells (MSC) into streptozotocin-treated NOD/SCID mice revealed endogenous islet regeneration occurred via distinct mechanisms. (uwo.ca)
  • Bone marrow stromal cells (BMSC) are multipotent stem cells and are considered good candidates for cell restoration in injured brain tissue. (sld.cu)
  • Bone marrow stromal cells (BMSC) are adult stem cells with characteristics that make them an attractive candidate in cellular therapy for neurological diseases (1). (sld.cu)
  • Endothelial progenitor cells (EPCs) are bone marrow-derived haematopoietic progenitor cells that have been implicated in vasculogenesis. (scirp.org)
  • Globally, it is estimated that 120 000 corneal transplantations and 18 000 transplantations of allogeneic haematopoietic progenitor cells took place in the year 2000. (who.int)
  • These cells were therefore described as bone marrow derived endothelial progenitor cells (EPCs). (hindawi.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Stimulates proliferation and differentiation of megakaryocytes from bone marrow progenitor cells, resulting in an increased production of platelets. (medscape.com)
  • Interacts with transmembrane domain of human TPO receptor and induces megakaryocyte proliferation and differentiation from bone marrow progenitor cells. (medscape.com)
  • Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages. (jove.com)
  • Retinoic acid modulates IL-5 receptor expression and selectively inhibits eosinophil-basophil differentiation of hemopoietic progenitor cells. (dadamo.com)
  • Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • Both human and animal models of diabetes and obesity were utilized to examine the use of stem cell intervention and gene therapy to amplify HO-1 levels. (nymc.edu)
  • Prolonged rhNELL-1 protein release was achieved using an apatite-coated β-tricalcium phosphate carrier, resulting in a local influx of stem cell antigen-1-positive (Sca-1+) mesenchymal progenitor cells (MPCs), and complete osseous fusion across all samples (100% spinal fusion rate). (nih.gov)
  • The AML that developed from these different cells of origin had different penetrance and aggressiveness when engrafted in mice, with the stem cell-derived lines being the most aggressive and the committed progenitor lines the least. (news-medical.net)
  • Together they identified open chromatin signatures and gene expression patterns in AML samples that may allow stem cell-derived AML to be distinguished from progenitor cell of origin AML. (news-medical.net)
  • So far, this has been impossible, as these cells retain their stem cell properties in their natural environment only, i.e. in their niche of the bone marrow. (disabled-world.com)
  • Stem cell reproduction therefore requires an environment similar to the stem cell niche in the bone marrow. (disabled-world.com)
  • The bone morrow contains several stem cell types including hematopoetic stem cells, endothelial progenitor cells, mesenchymal (stromal) stem cells and multipotent adult progenitor cells. (escardio.org)
  • Although there is no definitive evidence, combinations of progenitor cells seem to be more benefical than specific stem cell type for cardiac repair (3). (escardio.org)
  • The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)
  • It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola). (stemcell.com)
  • Human bone marrow CD34(+) hematopoietic progenitor cells (HPC) were exposed in vitro to 1,4-BQ to assess cytotoxicity, genotoxicity, and DNA damage responses and the molecular mechanisms associated with these events. (rti.org)
  • Granulocyte-macrophage colony-stimulating factor (GM-CSF) hypersensitivity of myeloid progenitors in vitro. (wikipedia.org)
  • The Procleix® Ultrio Elite Assay is a qualitative in vitro nucleic acid amplification test to screen for human immunodeficiency virus type 1 (HIV-1) RNA,hepatitis C virus (HCV) RNA, and/or hepatitis B virus (HBV) DNA, and detect human immunodeficiency virus type 2 (HIV-2) RNA in plasma and serum specimens from individual human donors, including donors of whole blood, blood components, and source plasma, and from other living donors. (fda.gov)
  • In vivo and in vitro data from both humans and animals indicate that benzene and/or its metabolites are genotoxic. (cdc.gov)
  • Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
  • Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
  • In experimental settings, the ability of human HSCs to reconstitute hematopoiesis is measured by transplantation into genetically immunocompromised mice (e.g. (stemcell.com)
  • Contrary to mixed-progenitor cells, transplantation of cultured MSC stably reduced hyperglycemia via an increase in islet number directly associated with the ductal epithelium without evidence of NGN3+ endocrine precursor activation. (uwo.ca)
  • In experimental and clinical studies unselected mononuclear bone marrow cells as well as specific subpopulations have been used for transplantation. (escardio.org)
  • Transplantation of human organs and tissues1 saves many lives and restores essential functions in circumstances when no medical alternative of comparable effectiveness exists. (who.int)
  • The number of human tissue transplants is increasing in both developed and developing countries, but global data on this form of transplantation are less complete. (who.int)
  • They began with five distinct, normal cell types found in the bone marrow in both mice and humans: long-term hematopoietic stem cells (HSCs), short-term HSCs, multipotent progenitors, common myeloid progenitors and granulocyte macrophage progenitors. (news-medical.net)
  • We show that a population of multipotent progenitors can be derived successfully from perivascular adipose tissue from patients with cardiovascular disease. (jove.com)
  • In preclinical and clinical studies, progenitor cell therapy (cord blood and mesenchymal stem cells) has shown promise in reversing the underlying pathology of SNHL, the loss of cochlear sensory hair cells. (intechopen.com)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
  • Nishiwaki, Y., Yoshida, M., Masuda, H. and Isobe, M. (2004) Recruitment of bone marrow-derived endothelial progenitor cells to vascular endothelium involves E-selectin dependent mechanism. (scirp.org)
  • We now need to study this pathway in humans, explore additional mechanisms by which proper sleep maintains vascular health and further explore this newly identified neuro-immune axis. (sciencedaily.com)
  • This protocol is used to answer key questions related to the function of human adipose progenitors in the vascular microenvironment. (jove.com)
  • The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. (beckman.com)
  • The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. (stemcell.com)
  • METHODS: Progenitor cells were obtained from bone marrow aspirates and cord blood samples. (dadamo.com)
  • To enrich for analogous human progenitor cells, we purified human umbilical cord blood (UCB) and BM based on high aldehyde dehydrogenase activity (ALDHhl), a conserved function in multiple progenitor lineages. (uwo.ca)
  • Then, the researchers introduced hematopoietic stem cells isolated from cord blood into this artificial bone marrow. (disabled-world.com)
  • The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. (jove.com)
  • Currently, little is known concerning regulation of IL-5Ralpha expression in the context of commitment of hemopoietic progenitor cells to the eosinophil and basophil lineages. (dadamo.com)
  • About 35% to 40% of CD34(+) and CD34(+)CD38(-) stem and progenitor cells adhered to laminin-10/11, and 45% to 50% adhered to fibronectin, whereas they adhered less to laminin-8 and laminin-1. (ox.ac.uk)
  • Human KG-1a cells (blue) and mouse EL4 T cells (red) stained with 148Nd-anti-CD34 (581). (biolegend.com)
  • In addition, the p21 mRNA level was elevated in 1,4-BQ-treated cells, suggesting that human CD34(+) cells utilize the p53 pathway in response to 1,4-BQ-induced DNA damage. (rti.org)
  • These results show that human CD34(+) cells are sensitive targets for 1,4-BQ toxicity that use the p53 DNA damage response pathway in response to genotoxic stress. (rti.org)
  • RESULTS: In semisolid methylcellulose cultures of normal human bone marrow, ATRA (10(-6) mol/L) selectively suppressed eosinophil-basophil colony-forming units but had no effect on granulocyte-macrophage colony-forming units. (dadamo.com)
  • Nivestym (filgrastim-aafi) is a recombinant human granulocyte colony-stimulating factor biosimilar to Neupogen indicated for the treatment of neutropenia associated with chemotherapy and related conditions. (drugs.com)
  • Recombinant human collagen XV regulates cell adhesion and migration. (nih.gov)
  • Here, recombinant human NELL-1 (rhNELL-1) implantation was examined in a clinically relevant nonhuman primate lumbar spinal fusion model. (nih.gov)
  • A) The biological activity of Human Recombinant G-CSF, ACF was tested by its ability to promote the proliferation of mouse NFS-60 cells. (stemcell.com)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • Different populations of progenitors have been shown to have conflicting functions. (rotrf.org)
  • This study could be important in developing risk-based surveillance for early outbreak detection to limit the spread of RVFV in both human and animal populations. (cdc.gov)
  • Obtain a 500 milligram piece of Human Perivascular Adipose Tissue or PVAT from the operating room as described in the text protocol. (jove.com)
  • Low power, H and E showing a hypocellular bone marrow with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
  • CD45 antibodies react with leucocyte progenitors in bone marrow. (beckman.com)
  • Murine studies revealed that Nell-1 haploinsufficiency results in marked reductions in the numbers of Sca-1+CD45-CD31- bone marrow MPCs associated with low bone mass. (nih.gov)
  • Conversely, rhNELL-1 systemic administration in mice showed a marked anabolic effect accompanied by increased numbers of Sca-1+CD45-CD31- bone marrow MPCs. (nih.gov)
  • The human bone marrow harbors a CD45− CD11B+ cell progenitor permitting rapid microglia‐like cell derivative approaches. (lu.se)
  • Three human cases were confirmed by polymerase chain reaction, and subsequent serological investigations revealed an overall IgG seropositivity of 13% in humans and 13% in animals. (cdc.gov)
  • The human immune system is composed of an innate and an adaptive branch. (hindawi.com)
  • Jubelirer SJ, Harpold R. The role of the bone marrow examination in the diagnosis of immune thrombocytopenic purpura: case series and literature review. (medscape.com)
  • As the main regulator of eosinopoiesis, eosinophil maturation and activation, and IgA production, IL5 contributes in several ways to human immune defenses against various pathogens, including helminths and infectious agents of the digestive and respiratory tracts. (dadamo.com)
  • The critical role of the immune system has been found in the discovery that there are reported high morbidity and mortality rates of human CoV infection in immunocompromised host as well as patients with comorbidities [ 3 - 6 ]. (medsci.org)
  • Blood cells, such as erythrocytes or immune cells, are continuously replaced by new ones supplied by hematopoietic stem cells located in a specialized niche of the bone marrow. (disabled-world.com)
  • In the second part of this review, we provide evidence that frequent exercise enhances-rather than suppresses-immune competency, and highlight key findings from human vaccination studies which show heightened responses to bacterial and viral antigens following bouts of exercise. (frontiersin.org)
  • Damage to both the humoral and cellular components of the immune system has been known to occur in humans following inhalation exposure. (cdc.gov)
  • We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
  • In summary, NELL-1 effectively induces bone formation across small and large animal models either via local implantation or intravenous delivery. (nih.gov)
  • NELL-1 induces an expansion of a bone marrow subset of MPCs with Sca-1 expression. (nih.gov)
  • Progenitor cells derived from bone marrow have been shown to respond following lung injury in both mice and humans. (rotrf.org)
  • We studied human bone marrow cell adhesion to laminin-10/11 (alpha5beta1gamma1/alpha5beta2gamma1), laminin-8 (alpha4beta1gamma1), laminin-1 (alpha1beta1gamma1), and fibronectin. (ox.ac.uk)
  • Bone marrow biopsy is performed in addition to aspiration to assess cellularity qualitatively and quantitatively. (medscape.com)
  • Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. (ox.ac.uk)
  • Since all these processes depend on the recruitment of cells with the potential to be committed to the osteoblastic lineage, we studied possible effects of IGF-I and -II on migration of human mesenchymal progenitor cells (MPC) using a modified Boyden chamber assay. (neuroprobe.com)
  • In conclusion, our results show that IGF-I and -II are chemotactic factors for MPC and indicate that IGFBP-5 both modulates the IGF-I effect and directly stimulates migration of human mesenchymal progenitor cells. (neuroprobe.com)
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • Further experiments revealed that the sleep-deprived mice had a nearly two-fold increase in the production in their bone marrow of stem cells that give rise to white blood cells. (sciencedaily.com)
  • While normally produced at high levels when animals -- including humans -- are awake, hypocretin levels were significantly reduced in the sleep-deprived mice. (sciencedaily.com)
  • High-dimensional approaches have revealed heterogeneity amongst dendritic cells (DCs), including a population of transitional DCs (tDCs) in mice and humans. (stanford.edu)
  • Relevant of mutagenicity and clastogenici- angiosarcomas of the liver, which carcinogens discussed in this chap- ty, including the induction of sister are rare tumours, were identified in ter do not include pharmaceutical chromatid exchange (SCE), chro- humans, rats, and mice exposed to drugs classified in Group 1, which mosomal aberrations (CA), and mi- vinyl chloride. (who.int)
  • or each of these agents, carcinogenicity in rats and/or mice, els, differences in exposure con- there was sufficient evidence of car- for example for the liver (aflatoxins, ditions between studies in animals cinogenicity from studies in rats and/ trichloroethylene [TCE], and vinyl and in humans, or limitations in Part 1 · Chapter 1. (who.int)
  • leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. (stemcell.com)
  • In addition we will examine the effect of immunosuppressive drugs on lung progenitor cell mobilization from the bone marrow using both clinical patient data and animal models of drug delivery, with the aim of determining specific protocols that optimize the patients own regenerative cell capacity and minimize the contribution of fibrotic cells. (rotrf.org)
  • A common clinical finding in benzene hematotoxicity is cytopenia, which is a decrease in various cellular elements of the circulating blood manifested as anemia, leukopenia, or thrombocytopenia in humans and in animals. (cdc.gov)
  • These criteria are identified through blood tests and bone marrow tests. (wikipedia.org)
  • This method is based upon experimental data which showed that the erythrocytes in bone marrow aspirates are mainly derived from the intravascular blood compartment. (karger.com)
  • We have discovered that sleep helps to regulate the production in the bone marrow of inflammatory cells and the health of blood vessels and that, conversely, sleep disruption breaks down control of inflammatory cell production, leading to more inflammation and more heart disease," says Filip Swirski, PhD, of the MGH Center for Systems Biology, senior author of the Nature paper. (sciencedaily.com)
  • Researchers are investigating several approaches, such as enzymatic treatments of RBCs, the generation of RBCs from human induced pluripotent stem cells, and the development of artificial oxygen carriers, all with the goal of advancing universal blood. (cap.org)
  • With the help of synthetic polymers, the scientists created a porous structure simulating the sponge-like structure of the bone in the area of the blood-forming bone marrow. (disabled-world.com)
  • The company has so far tested the PKU drug in animals and in human blood in the lab, and it aims to begin clinical testing next year. (technologyreview.com)
  • The new technology draws on recent advances in the ability to genetically modify and grow human red blood cells from stem cells in culture. (technologyreview.com)
  • Using established molecular biology techniques, Rubius's scientists can engineer progenitor cells taken from human bone marrow and grow blood cells that produce specific therapeutic proteins on their surface or inside the cell. (technologyreview.com)
  • Human red blood cells circulate for as long as four months, meaning they could potentially form the basis of long-term therapies. (technologyreview.com)
  • This disorder primarily affects the bone marrow, which produces new blood cells. (medlineplus.gov)
  • Studies indicate that a shortage of functioning ribosomes may increase apoptosis of blood-forming cells in the bone marrow, resulting in a low number of red blood cells (anemia). (medlineplus.gov)
  • Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
  • Antibodies are found in many vertebrates, including humans. (medscape.com)
  • In October, 2009, 23 scientists from six countries met at the International Agency for Research on Cancer (IARC) to reassess the carcinogenicity of several chemical and occupational exposure circumstances previously classified as "carcinogenic to humans" (Group 1) and to identify additional tumour sites and mechanisms of carcinogenesis. (cdc.gov)
  • In addition, carcinogenic to humans . (who.int)
  • Calpin C, Dick P, Poon A, Feldman W. Is bone marrow aspiration needed in acute childhood idiopathic thrombocytopenic purpura to rule out leukemia? (medscape.com)
  • Myeloid cells are a heterogeneous population of bone marrow-derived cells that play a critical role during growth and metastasis of malignant tumors. (hindawi.com)
  • 1- Immediately after receiving my Ph.D. degree, my postdoctoral fellowship at the Rockefeller University focused on the purification of a human liver protein (heme oxygenase, HO-1) and its gene sequence, including the promoter region. (nymc.edu)
  • In addition, they added protein building blocks similar to those existing in the matrix of the bone marrow for the cells to anchor. (disabled-world.com)
  • Specifically, Clara cell secretory protein (CCSP) + epithelial progenitors contribute to repair and lung maintenance, while collagen-1 + fibroblast progenitors ( fibrocytes ) are associated with lung fibrosis, impaired lung function, and reduced survival. (rotrf.org)
  • Her lab worked with a mouse model of acute myeloid leukemia (AML) driven by expression of MLL-AF9, a fusion oncogene formed by a chromosome translocation between human chromosomes 9 and 11. (news-medical.net)
  • In the past dozen years, the outbreak of the novel coronavirus is the third on record spillover of an animal coronavirus to humans after occurring of severe acute respiratory syndrome (SARS) started in 2002 and Middle East respiratory syndrome coronavirus (MERS-CoV) in 2014, which is resulting in a pandemic in global. (medsci.org)
  • Benzene has also been associated with acute non-lymphocytic leukemia in humans, and aplastic anemia may be an early indicator of developing acute non-lymphocytic leukemia in some cases. (cdc.gov)
  • Nipah virus (NiV) causes a highly lethal disease in humans who present with acute respiratory or neurological signs. (cdc.gov)
  • Benzene also causes a life-threatening disorder called aplastic anemia in humans and animals. (cdc.gov)
  • The clinical presentation of patients with aplastic anemia includes symptoms related to the decrease in bone marrow production of hematopoietic cells (see the image below). (medscape.com)
  • Paul Ehrlich introduced the concept of aplastic anemia in 1888 when he studied the case of a pregnant woman who died of bone marrow failure. (medscape.com)