• Grebe T , Hakenbeck R . Penicillin-binding proteins 2b and 2x of Streptococcus pneumoniae are primary resistance determinants for different classes of beta-lactam antibiotics. (cdc.gov)
  • A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene. (nih.gov)
  • Aberrant promoter methylation of insulin-like growth factor binding protein-3 gene in human cancers. (nih.gov)
  • In summary, our results showed that IGFBP-3 methylation played an important role in the silencing of its expression, suggesting that IGFBP-3 may act as a tumor suppressor gene in several human cancers examined. (nih.gov)
  • Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. (wikipedia.org)
  • Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. (wikipedia.org)
  • This gene is also a candidate tumor suppressor gene for human breast cancer. (wikipedia.org)
  • We identified a novel SNP variant, Chr12:g.53494591T>C. c.T430C (p.S144P) in the insulin-like growth factor binding protein-6 (IGFBP6) gene. (cusabio.com)
  • This gene encodes a protein with a conserved RNA-binding motif. (beds.ac.uk)
  • There are two alternate translation start codons with this gene, which result in two forms of the protein. (beds.ac.uk)
  • Recombinant adeno-associated viruses (rAAVs) hold enormous potential for human gene therapy. (duke.edu)
  • Mutations in the human MECP2 gene cause the autism spectrum disorder Rett Syndrome. (portlandpress.com)
  • Surprisingly, these symptoms are efficiently reversed by delayed activation of a 'stopped' Mecp2 gene, raising hopes that human Rett syndrome may also be reversible. (portlandpress.com)
  • Gene regulation in the human genome is controlled by distal enhancers that activate specific nearby promoters 1 . (nature.com)
  • Together, this systematic assessment of enhancer-promoter compatibility suggests a multiplicative model tuned by enhancer and promoter class to control gene transcription in the human genome. (nature.com)
  • The TNFRSF1A gene provides instructions for making a protein called tumor necrosis factor receptor 1 (TNFR1). (medlineplus.gov)
  • Additionally, because only one copy of the TNFRSF1A gene has a mutation, some normal TNFR1 proteins are produced and can bind to the TNF protein, leading to additional inflammation. (medlineplus.gov)
  • The Legionella SH2 domains feature the SH2 fold and a pTyr-binding pocket, but lack a specificity pocket found in a typical mammalian SH2 domain for recognition of sequences flanking the pTyr residue. (rcsb.org)
  • Broad Genomics Platform sequences a whole human genome every four minutes. (broadinstitute.org)
  • It is a nuclear protein that is largely chromatin-bound and has a strong preference for binding to methylated DNA sequences in vivo . (portlandpress.com)
  • Here we design a high-throughput reporter assay called enhancer × promoter self-transcribing active regulatory region sequencing (ExP STARR-seq) and applied it to examine the combinatorial compatibilities of 1,000 enhancer and 1,000 promoter sequences in human K562 cells. (nature.com)
  • The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)
  • abstract = "Secreted noggin protein regulates bone morphogenetic protein activity during development. (elsevierpure.com)
  • In the end, what they found is that MMS does, in fact, inactivate the binding of covid variant spike proteins to the human ACE2 receptor protein, "indicating that this strategy may be useful in blocking the transmission of variant SARS-CoV-2 viruses. (naturalnews.com)
  • For their experiments, the team sprayed a 50 ?L aliquot of chlorine dioxide or chlorine dioxide spray solution diluted to the appropriate concentration onto microtiter plates containing purified Receptor-Binding Domain (RBD) spike proteins. (naturalnews.com)
  • Here we report two monoclonal antibodies (mAbs) cloned from memory B cells of patients recently recovered from COVID-19, and both mAbs specifically bind to the spike (S) protein of SARS-CoV-2, block the binding of receptor binding domain (RBD) of SARS-CoV-2 to human angiotensin converting enzyme 2 (hACE2), and effectively neutralize S protein-pseudotyped virus infection. (medrxiv.org)
  • Engagement of the highly polymorphic domain II of the P. vivax Duffy-binding protein (DBPII) with the erythrocyte's Duffy Ag receptor for chemokines (DARC) is essential. (tropmedres.ac)
  • This suggests that the immunophilin-heat shock protein-steroid receptor interaction is ubiquitous and not limited to immune systems. (broadinstitute.org)
  • We report that serotypes rAAV-1, -5, and -6 but not serotypes rAAV-2, -7, -8, -9, and -10 interact in human sera with galectin 3 binding protein (hu-G3BP), a soluble scavenger receptor. (duke.edu)
  • The invasion of reticulocytes by Plasmodium vivax merozoites is dependent on the interaction of the Plasmodium vivax Duffy Binding Protein (PvDBP) with the Duffy antigen receptor for chemokines (DARC). (bvsalud.org)
  • Second messenger signaling bypasses CGRP receptor blockade to provoke migraine attacks in humans. (medscape.com)
  • Interaction of organochlorine compounds with thyroid receptor, transthyretin, and thyroid-binding globulin. (cdc.gov)
  • The antibodies bind with high affinity in the central region of the surface-bound M protein. (lu.se)
  • In this study, the binding affinity and mechanism of human odor-binding protein OBP2a to 14 typical odorants in water were first assessed using fluorescent competitive binding assays and molecular docking techniques. (springeropen.com)
  • The binding affinity of OBP2a to fatty aldehydes including hexanal, heptanal, 2-octenal, decanal, and β-cyclocitral increased with the increase of hydrophobicity of aldehydes. (springeropen.com)
  • The conformation of the active site of the enzyme is a key determinant of binding affinity ( K m ), so we also wanted to investigate metabolism in an intact cellular system in which the enzyme is maintained as close as possible to the environment in situ . (aspetjournals.org)
  • Relative penicillin G resistance in Neisseria meningitidis and reduced affinity of penicillin-binding protein 3. (cdc.gov)
  • To better understand the functional characteristics and fine specificity of protective human Abs to DBPII, we sorted single DBPII-specific IgG+ memory B cells from three individuals with high blocking activity to DBPII. (tropmedres.ac)
  • The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
  • The hybridization LC-MS/MS platform provided unique advantages, including minimal matrix effects and high specificity, compared with traditional ligand binding assays or liquid scintillation approaches, which enabled efficient and reliable in vitro protein binding assay. (aspetjournals.org)
  • We demonstrate for the first time that the peroxisomal ABC proteins can be expressed in Sf21 membranes maintaining their nucleotide-binding abilities and ATPase activities, and the expressed proteins will be of use for further characterization. (nih.gov)
  • Identification and Characterization of Functional Human Monoclonal Antibodies to Plasmodium vivax Duffy-Binding Protein. (tropmedres.ac)
  • Olfactory sensors based on odor-binding proteins (OBPs) have been utilized to analyze pollutants in food and air samples, while their application for the detection of typical odor-causing compounds in drinking water is rarely reported, partly due to the lack of knowledge about the binding properties of odorants. (springeropen.com)
  • Odorant binding proteins (OBPs) are involved in either olfactory or nonolfactory cue sensation depending on their spatial and temporal expression. (bioone.org)
  • Remarkably, this antibody primarily interacts through a bivalent dual-Fab cis mode, where the Fabs bind to two distinct epitopes in the M protein. (lu.se)
  • FABP3 is known to interact with TNNI3K in the context of interacting with cardiac troponin I. The protein also interacts with, VPS28, KIAA159, NUP62, PLK1, UBC, and Xpo1. (wikipedia.org)
  • Scholars@Duke publication: Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6. (duke.edu)
  • It interacts also with anticoagulant protein S and with serum amyloid P component. (lu.se)
  • Group A streptococci have evolved multiple strategies to evade human antibodies, making it challenging to create effective vaccines or antibody treatments. (lu.se)
  • In contrast, other antibodies binding with normal single-Fab mode to the same region cannot bypass the M protein's virulent effects. (lu.se)
  • Monoclonal antibodies specific for HSP47, type III collagen and type IV collagen were used to assess the relative expression of their proteins in paraffin-embedded renal sections by immunohistochemistry. (scienceopen.com)
  • Moreover, antibodies elicited by the two adjuvant formulations had similar functional properties such as avidity, isotype profile and inhibition of PvDBPII-DARC binding. (bvsalud.org)
  • Furthermore, the anti-PvDBPII antibodies were able to block the interaction of DARC with the homologous PvDBPII SalI allele as well as the heterologous PvDBPII PvW1 allele from a Thai clinical isolate that is used for controlled human malaria infections (CHMI). (bvsalud.org)
  • A broadly binding, protective monoclonal antibody could be a candidate for anti-streptococcal therapy. (lu.se)
  • Our findings highlight the concept of dual-Fab cis binding as a means to access conserved, and normally non-opsonic regions, regions for protective antibody targeting. (lu.se)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Calcyclin Binding Protein (CACYBP) in Tissue homogenates, cell lysates and other biological fluids. (glideruniversity.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Calcyclin Binding Protein (CACYBP) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (glideruniversity.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) in Tissue homogenates, cell lysates and other biological fluids. (worldcarecouncil.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (worldcarecouncil.org)
  • The bacterial SH2 domains are not only capable of binding proteins or peptides in a Tyr phosphorylation-dependent manner, some bind pTyr itself with micromolar affinities, a property not observed for mammalian SH2 domains. (rcsb.org)
  • Description: CACYBP Human Recombinant produced in E.Coli is a single, non-glycosylated,_x000D_ polypeptide chain containing 205 amino acids (1-185a.a.) and having a molecular mass of 23.4 kDa. (glideruniversity.org)
  • Sample: Recombinant FKBP5, Human. (uscnk.com)
  • This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)
  • The commercial introduction of recombinant human growth hormone (rhGH) in 1985 dramatically changed the field of therapy for growth hormone (GH). (medscape.com)
  • They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. (wikipedia.org)
  • The effect of bovine serum albumin (BSA) on human liver metabolism, in vitro , of 14 C-phenytoin (PHT) was studied. (aspetjournals.org)
  • Heat shock protein (HSP) 47 has been identified as collagen-binding stress protein, shown to have a specific role in the intracellular processing of procollagen molecules during collagen assembly. (scienceopen.com)
  • The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. (wikipedia.org)
  • 1994). High-res- belongs to the family of intracellular lipid bind- olution crystal structures (Sacchetti ni et al. (lu.se)
  • In HIV, a synthetic peptide corresponding to the immunosuppressive domain (amino acids 574-592) of HIV-1 gp41 downregulates the expression of fatty acid binding protein 3 (FABP3) in peptide-treated PBMCs. (wikipedia.org)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • Recently, we showed that p59 purified from human lymphocytes is an immunophilin (FKBP59) which binds both FK506 and rapamycin. (broadinstitute.org)
  • A FKBP59 homologue complexed with hsp90-hsp70 was also detected in yeast, which suggests that the immunophilin-heat shock protein association has been evolutionarily conserved. (broadinstitute.org)
  • Molecular docking studies indicated that all three complexes primarily bind to HSA in subdomain IIA with amino acid residues such as Arg218 and Lys199 which are located at the entrance of Sudlow's site I. The in vitro cytotoxicities of complexes 1-3 against HeLa cells showed promising anticancer activity (IC 50 = 3.5, 3.9 and 16.9 μM for 1 , 2 and 3 , respectively). (rsc.org)
  • We identified 12 DBPII-specific human mAbs from distinct lineages that blocked DBPII-DARC binding. (tropmedres.ac)
  • All mAbs were P. vivax strain transcending and targeted known binding motifs of DBPII with DARC. (tropmedres.ac)
  • The N-terminal cysteine -rich region II of PvDBP (PvDBPII), which binds DARC, is a leading P. vivax malaria vaccine candidate. (bvsalud.org)
  • Owing to their physical properties, such as relatively high molecular weight, linear structure, and nonspecific binding, ASOs present unique challenges in ƒ u determination through traditional techniques. (aspetjournals.org)
  • SIGNIFICANCE STATEMENT A novel hybridization LC-MS/MS-based approach was successfully developed for the determination of ASO in vitro protein binding in plasma, and for the first time brain and CSF. (aspetjournals.org)
  • The K m u values determined in the presence of albumin in both microsomes and slices were similar to those based on in vivo human steady state data ( K m u = 2-3 μM), and the intersubject variation, in vitro , was decreased in the presence of BSA. (aspetjournals.org)
  • Because phenytoin is principally bound to albumin, we also explored the effect of albumin on the V max and K m values obtained, in vitro . (aspetjournals.org)
  • We utilized this reaction to determine the amount of cysteine-bound styrene-7,8-oxide (SO) which reacted with Hb in vitro and in vivo. (cdc.gov)
  • In addition, we found evidence to indicate that conformation as well as sequence was important for good hexon binding in our assays. (microbiologyresearch.org)
  • Inside the cell, these proteins clump together and are thought to trigger alternative pathways that initiate inflammation. (medlineplus.gov)
  • The clumps of protein constantly activate these alternative inflammation pathways, leading to excess inflammation in people with TRAPS. (medlineplus.gov)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • In mice, a complete loss of noggin protein leads to multiple malformations including joint fusion, whereas mice heterozygous for Nog loss-of-function mutations are normal. (elsevierpure.com)
  • In humans, heterozygous NOG missense mutations have been found in patients with two autosomal dominant disorders of joint development, multiple synostosis syndrome (SYNS1) and a milder disorder proximal symphalangism (SYM1). (elsevierpure.com)
  • These data indicate that the human disease-causing mutations are hypomorphic alleles that reduce secretion of functional dimeric noggin. (elsevierpure.com)
  • Most of these mutations lead to changes in single protein building blocks (amino acids), typically involving the amino acid cysteine. (medlineplus.gov)
  • In November 2022, Broad's Genomics Platform sequenced its 500,000th whole human genome, a mere four years after sequencing its 100,000th. (broadinstitute.org)
  • However, a comprehensive genome-wide map of human S/MARs is yet not available. (researchgate.net)
  • Toward this objective, ChIP-Seq data of 14 S/MAR binding proteins were analyzed and the binding site coordinates of these proteins were used to prepare a non-redundant S/MAR dataset of human genome. (researchgate.net)
  • An integrated encyclopedia of DNA elements in the human genome. (nature.com)
  • CSL controls telomere maintenance and genome stability in human dermal fibroblasts. (harvard.edu)
  • MIR-1265 regulates cellular proliferation and apoptosis by targeting calcium binding protein 39 in gastric cancer and, thereby, impairing oncogenic autophagy. (nih.gov)
  • Signaling from the TNFR1 protein can trigger either inflammation or self-destruction of the cell (apoptosis). (medlineplus.gov)
  • Apoptosis is initiated when the TNFR1 protein, bound to the TNF protein, is brought into the cell and starts a process known as the caspase cascade. (medlineplus.gov)
  • Homo sapiens (Human) USCN(Wuhan USCN Business Co., Ltd. (uscnk.com)
  • Historically, ƒ u determination for small-molecule drug candidates has been determined by ultrafiltration, ultracentrifugation, or equilibrium dialysis, which are techniques based on the physical separation of unbound drug molecules from those bound to proteins ( Pacifici and Viani, 1992 ). (aspetjournals.org)
  • Human serum albumin (HSA) binding of anticancer plant alkaloid ellipticine has been studied in conjunction with assessing its association to serum a1-acid-glycoprotein (AAG). (jascoinc.com)
  • C(H)-C 6 H 4 OH- o , o -phen = 1,10-phenanthroline, and OAc = CH 3 COO − ) with human serum albumin (HSA) was studied using fluorescence quenching, synchronous and 3D fluorescence measurements and UV-vis spectroscopy. (rsc.org)
  • The interaction of the TNF protein with the TNFR1 protein causes the TNFR1 protein to bind to two other TNFR1 proteins, forming a three-protein complex called a trimer. (medlineplus.gov)
  • When cysteines within the TNFR1 protein are replaced with other amino acids, the disulfide bonds are not formed, and the protein is misfolded. (medlineplus.gov)
  • It shares homology with the mating-type protein, Mc, from the fission yeast Schizosaccharomyces pombe and a conserved DNA-binding motif present in the nuclear high-mobility-group proteins HMG1 and HMG2. (nih.gov)
  • These agents are characterized by their ability to bind covalently to nucleophilic sites in proteins and DNA. (cdc.gov)
  • Immunogenicity of a Plasmodium vivax vaccine based on the duffy binding protein formulated using adjuvants compatible for use in humans. (bvsalud.org)
  • Signaling within the cell initiates a pathway that turns on a protein called nuclear factor kappa B, which triggers inflammation and leads to the production of immune system proteins called cytokines. (medlineplus.gov)
  • Src homology 2 (SH2) domains play a critical role in signal transduction in mammalian cells by binding to phosphorylated Tyr (pTyr). (rcsb.org)
  • In mice, rAAV-6 interacted with another protein of the innate immune system, C-reactive protein (CRP). (duke.edu)
  • Outside the cell, the TNFR1 protein attaches (binds) to another protein called tumor necrosis factor (TNF). (medlineplus.gov)
  • A paper published in the journal Annals of Pharmacology and Pharmaceutics explains how MMS blocks the spike proteins associated with SARS-CoV-2 that are said to bind with human receptors such as Angiotensin-Converting Enzyme 2 (ACE2), allowing disease to form. (naturalnews.com)
  • The stated purpose of the study was to see if chlorine dioxide directly inhibits the binding of the Chinese Virus Spike protein (S protein) to ACE2 receptors. (naturalnews.com)
  • It may also be useful in helping the vaccinated and those who test "positive" for the China Virus to rid themselves of spike proteins by preventing them from attaching to ACE2 receptors. (naturalnews.com)
  • P59 [also known as FK506 binding protein 59 (FKBP59) or heat shock protein 56 (hsp56)] and heat shock proteins 90 and 70 (hsp90 and hsp70) associate with steroid receptors and are believed to maintain the receptors in an inactive state. (broadinstitute.org)
  • Here we provide evidence that rabbit uterine p59 also binds FK506 and rapamycin and that p59 or its homologue is associated with nontransformed progesterone receptors of rabbit uterus and chicken oviduct. (broadinstitute.org)
  • 3D fluorescence studies showed that the HSA structure was altered at the secondary and tertiary levels upon binding with the complexes. (rsc.org)
  • The average binding distance ( r ) between HSA and the complexes was obtained by Förster's resonance energy transfer theory. (rsc.org)
  • In addition, we found that the FKBP59-hsp complexes are more complicated than previously thought, involving other proteins such as actin and a 63-kDa protein, p63. (broadinstitute.org)
  • HFABP, heart muscle fatty acid binding binding and release is well understood. (lu.se)
  • This trimer formation is necessary for the TNFR1 protein to be functional. (medlineplus.gov)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • Description: A sandwich ELISA kit for detection of High Density Lipoprotein Binding Protein from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (tuberculosistest.net)
  • Description: A sandwich ELISA kit for detection of Cold Shock Domain Containing Protein E1, RNA Binding from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (worldcarecouncil.org)
  • Binding Elisa Laboratories manufactures the s-protein binding elisa reagents distributed by Genprice. (mugen-noe.org)
  • The S-Protein Binding Elisa reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (mugen-noe.org)
  • Description: A competitive ELISA for quantitative measurement of Human True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (mugen-noe.org)
  • Description: A sandwich ELISA for quantitative measurement of Goat Mannma binding protein/mannan binding lectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (mugen-noe.org)
  • The valuable information to the binding of OBP2a to typical odorants in this study would provide a theoretical foundation for the development of OBP-based odor detection biosensors to achieve quick detection in drinking water, further helping the improvement of water treatment processes in the water industry. (springeropen.com)
  • The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
  • Eleven mAbs competed with each other for binding, indicating recognition of the same or overlapping epitopes. (tropmedres.ac)
  • Our work expands the boundary of phosphotyrosine signalling to prokaryotes, suggesting that some bacterial effector proteins have acquired pTyr-superbinding characteristics to facilitate bacterium-host interactions. (rcsb.org)
  • Adenovirus protein- protein interactions: hexon and protein VI. (microbiologyresearch.org)
  • Deciphering protein–protein interactions. (crossref.org)
  • Based on these data, we propose that species-specific interactions of AAVs with blood proteins may differentially impact vector distribution and efficacy in different animal models. (duke.edu)
  • Although ASO protein binding has been previously characterized in plasma, there were no studies that quantitated ASO ƒ u in brain or cerebral spinal fluid (CSF). (aspetjournals.org)
  • Taking advantage of the ability of chiral protein environments to induce optical activity, circular dichroism (CD) spectroscopy was employed to characterize the alkaloid-protein non-covalent adducts. (jascoinc.com)
  • CACYBP protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography. (glideruniversity.org)
  • Binding of serum folic acid activity (FAA) to the protein fractions during and after pregnancy was studied by DEAE Sephadex A-50 gel chromatography. (karger.com)
  • We screened for serum proteins interacting with different AAV serotypes in humans, macaques, dogs, and mice. (duke.edu)
  • MeCP2 is most highly expressed in neurons, and mice lacking this protein show symptoms that strikingly parallel those of Rett patients. (portlandpress.com)
  • Heart-type Fatty Acid-Binding Protein (H-FABP) is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following an ischemic episode. (wikipedia.org)
  • This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. (nih.gov)
  • Spike protein coated onto a microtiter plate was treated with chlorine dioxide aqueous solution or chlorine dioxide spray solution," the study explains. (naturalnews.com)
  • Can chlorine dioxide protect your cells against a spike protein invasion? (naturalnews.com)
  • For everyone else, taking MMS could provide a shield of protection against the type of spike protein damage that is causing some people to suffer cardiovascular events or worse. (naturalnews.com)
  • The higher the concentration of chlorine dioxide, the fewer spike protein binding events were observed. (naturalnews.com)