• Nrf2/ARE pathway attenuates oxidative and apoptotic response in human osteoarthritis chondrocytes by activating ERK1/2/ELK1-P70S6K-P90RSK signaling axis. (metu.edu.tr)
  • Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (CPA). (ox.ac.uk)
  • Current work involves relating findings in mouse models of osteoarthritis to aspects of the human disease by examining knockout and transgenic mouse strains in the context of a post-traumatic OA mouse model due to surgical destabilization of the medial meniscus (DMM). (hss.edu)
  • In osteoarthritis (OA) articular chondrocytes undergo phenotypic changes culminating in the progressive loss of cartilage from the joint surface. (uwo.ca)
  • Osteoarthritis (OA) is a multi-factorial disease leading progressively to loss of articular cartilage and subsequently to loss of joint function. (biomedcentral.com)
  • The authors conclude that the quinolone arthropathy induced in rabbits in the present study is consistent with that seen in other experimental animals and represents a good model of osteoarthritis in humans. (cdc.gov)
  • The current view holds that osteoarthritis involves not only the articular cartilage but the entire joint organ, including the subchondral bone and synovium. (medscape.com)
  • Intra-articular knee injections of hyaluronic acid have been shown to provide functional and perceived benefits in knee osteoarthritis for up to 5-6 months. (medscape.com)
  • Intra-articular injection of botulinum neurotoxin A into the knee joint may provide therapeutic pain relief in patients with advanced knee osteoarthritis. (medscape.com)
  • According to a preliminary study, pain and stiffness significantly improved and lasted about 3 months following intra-articular knee joint botulinum toxin A injection, though physical function did not significantly improve based on the Western Ontario McMaster Universities Osteoarthritis Index. (medscape.com)
  • Intra-articular knee injections of homologous platelet-rich plasma (PRP) have been shown to improve function and quality of life in patients with degenerative lesions of the knee cartilage and osteoarthritis at 6 months post injection. (medscape.com)
  • Variations in chondrocyte density and organization in cartilage histology sections are associated with osteoarthritis progression. (cdc.gov)
  • Compared with intact cored discs, dicing of AC tissue into 0.2-1 mm cubes significantly increased the viability and proliferative capacity of surviving chondrocytes after cryopreservation. (ox.ac.uk)
  • In conclusion, modification of appropriate factors, such as the size of the tissue, cryoprotective agent, and isolation protocol, can allow successful isolation of viable chondrocytes with high proliferative capacity from cryopreserved human articular cartilage tissue. (ox.ac.uk)
  • There are two main options for medication therapies, which are the drugs for treating symptomatic pain and intra-articular (IA) injection. (frontiersin.org)
  • Intra-articular injection of hyaluronic acid (HA) or corticosteroids was also shown to improve pain, whereas the HA administration showed more prolonged effect [ 10 , 11 ]. (biomedcentral.com)
  • The most common type of intra-articular knee injection is with corticosteroids, but other agents have been used, including infliximab, hyaluronic acid, botulinum neurotoxin, and platelet-rich plasma (PRP). (medscape.com)
  • Intra-articular infliximab can be used to treat refractory knee monoarthritis/synovitis in patients with rheumatoid arthritis, Behçet disease, and spondyloarthropathy (eg, ankylosing spondylitis) that is resistant to systemic treatment. (medscape.com)
  • Intra-articular hyaluronic acid injection into a rheumatoid arthritic knee can modulate inflammatory changes, though the exact mechanism or mechanisms are unclear. (medscape.com)
  • Currently, the only FDA-approved cell-based therapy for cartilage defects involves autologous chondrocyte implantation: chondrocytes harvested from low-contact areas are expanded in vitro and then re-injected directly into the damaged site 1 . (nature.com)
  • Anteroposterior (AP) radiograph of the hip reveals severe superior migration of the femoral head (which reflects loss of articular cartilage), subchondral sclerosis, prominent osteophytes, and a large Egger cyst in the superior acetabulum. (medscape.com)
  • Co-culture of mesenchymal stromal cells (MSCs) with articular chondrocytes (ACs) has been reported to improve the efficiency of utilization of a small number of ACs for the engineering of implantable cartilaginous tissues. (unibas.ch)
  • MSCs can be differentiated along different cell lineages of mesodermal origin including osteoblasts, chondrocytes, skeletal myocytes or visceral stromal cells 9 . (nature.com)
  • Korpershoek JV, Rikkers M, Wallis FSA, Dijkstra K, Te Raa M, de Knijff P, Saris DBF , Vonk LA. Mitochondrial Transport from Mesenchymal Stromal Cells to Chondrocytes Increases DNA Content and Proteoglycan Deposition In Vitro in 3D Cultures. (mayo.edu)
  • Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. (nature.com)
  • Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
  • Morscheid S., Rey-Rico A., Schmitt G., Madry H., Cucchiarini M., Venkatesan J.K. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-β and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells. (uniklinikum-saarland.de)
  • Our group of scientists and clinicians has been continuously funded by the California Institute of Regenerative Medicine (CIRM) to develop a first-in class pluripotent stem cell-based therapy for focal articular cartilage lesions. (ca.gov)
  • The work described in this proposal is designed to produce a universal treatment for articular cartilage lesions. (ca.gov)
  • One recently developed reagent recognizes a molecule associated with human pancreatic cancer cells as well as precancerous lesions of the pancreas. (ohsu.edu)
  • The use of chondrocytes in cell-based therapies for cartilage lesions is limited by quantity and requires an in vitro 2D expansion, which leads to cell dedifferentiation. (intechopen.com)
  • The model involved the induction of degenerative articular lesions in New-Zealand-White-rabbits by the injection of nalidixic-acid (389082) (NA). (cdc.gov)
  • Histochemical changes in the cartilaginous matrices within the articular lesions were seen at all stages of lesion development. (cdc.gov)
  • Autologous chondrocytes implantation and osteochondral autograft transplantation are limited by scarce cartilage production, low proliferative capacity of chondrocytes, chondrocyte de-differentiation and complications due to donor site morbidity 5 . (nature.com)
  • The purpose of this study is to evaluate the feasibility of human amniotic membrane (HAM) as a chondrocyte carrier by assessing cell proliferation and maintenance of phenotype in vitro and cartilage regeneration in vivo. (nih.gov)
  • These results suggested that denuded HAM was able to support chondrocyte proliferation and maintenance of phenotype in vitro, seemingly more favorable when DHS was used. (nih.gov)
  • This study provides evidence for further testing on the molecular mechanism of the disease and also propose proteins as candidate markers of OA chondrocyte phenotype. (biomedcentral.com)
  • [ 16 ] Additionally, PRP injections have shown greater and longer efficacy than hyaluronic acid injections in reducing pain and symptoms and improved articular function. (medscape.com)
  • We performed a quantitative proteomic analysis in osteoarthritic and normal chondrocytes followed by functional analyses to investigate proteome changes and molecular pathways involved in OA pathogenesis. (biomedcentral.com)
  • In this study we compared the quantitative changes in proteins synthesized in osteoarthritic compared to normal chondrocytes. (biomedcentral.com)
  • Here we investigated the in vitro and in vivo chondrogenic capacities of scaffold-based constructs generated by combining primary human ACs with human bone marrow MSCs (BM-MSCs). (unibas.ch)
  • For AC tissue cryopreservation, intact cored cartilage discs (5 mm diameter) and diced cartilage (0.2-1 mm cubes) from the same sized discs were step cooled and stored in liquid nitrogen for up to 48 h before chondrocyte isolation and in vitro assay of cell viability and proliferative potential. (ox.ac.uk)
  • Mechanistic studies involve in vitro experiments using tissues and cells derived from the mouse strains and human samples retrieved from surgical procedures. (hss.edu)
  • Chondrocyte apoptosis and expression of Bcl-2, Bax, Fas, and iNOS in articular cartilage in patients with Kashin-Beck disease. (jrheum.org)
  • LCN2 was increased by SOX9 and SIRT1 deacetylated SOX9 to promote its nuclear translocation, which further inhibited the viability of human retinal pigment epithelial cells and promoted cell apoptosis and angiogenesis as well as CNV-induced AMD formation. (hindawi.com)
  • 4] Naranda J, Gradišnik L, Gorenjak M, Vogrin M, Maver U. Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA). (metu.edu.tr)
  • Characterization and activation of procollagenase from human polymorphonuclear leucocytes. (wikipedia.org)
  • Further studies are required to determine whether these cells may retain cartilage differentiation capacity and provide sufficient chondrocytes for use as implants in clinical applications. (ox.ac.uk)
  • L-NMA completely reversed the suppression of proteoglycan synthesis imposed by IL-1 in deep chondrocytes, but produced only partial reversal in superficial cells. (elsevierpure.com)
  • In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. (karger.com)
  • While chondrocytes grew in monolayer fashion on the surface of IHE and DHB substrates, the cells seeded in DHS penetrated and spread into the whole thickness of the stromal layer. (nih.gov)
  • The most attractive cell types currently used for CTE are autologous chondrocytes and adult stem cells. (intechopen.com)
  • In the present chapter, we report the development of heparin-, chondroitin sulfate-, decorin-, and poly(ε-caprolactone)-based self-assembling peptide composite scaffolds to promote re-differentiation of expanded human articular chondrocytes and induction of adipose-derived stem cells to chondrogenic commitment. (intechopen.com)
  • Methods Chondrocytes were isolated from the joint cartilages of OA patients (grade 3-4) during total knee arthroplasty with the approval of Kecioren Training and Research Hospital, Clinical Research Ethics Committee (B.10.4.İSM.4.06.68.49), as described previously.⁴ The alterations in cell counts, viability (MTT, NRU), proliferation (RTCA-iCELLigence System), reactive oxygen species (ROS) generation, stress-related signalling proteins and inflammatory progenitors ICE/caspase-1 (ELISA) were determined. (metu.edu.tr)
  • This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. (ox.ac.uk)
  • Methods: Bovine articular chondrocytes were expanded and transferred into pellet culture at passage 3. (bvsalud.org)
  • Histologic and immunohistochemical evaluations indicated a higher differentiation potential of chondrocytes treated with 84-EVi. (bvsalud.org)
  • Summary/conclusion: ADSCs enhances chondrogenesis and matrix synthesis of human articular chondrocytes is mediated by EVsantimicrobial action test, Staphylococcus aureus (S. aureus) was cultured in LB broth medium at 37, O/ N. The seed culture ratio (1/100, 1/1000) and different exosome concentration were inoculated and development was confirmed by time. (achrinhibitor.com)
  • This pilot study aimed to investigate the effect of characterized MSC-EVs on chondrogenesis in a 3D chondrocyte inflammation model with the pro-inflammatory cytokine TNFα. (bvsalud.org)
  • The results showed that 10 % dimethyl sulphoxide in 90 % foetal bovine serum was a successful CPA for chondrocyte cryopreservation. (ox.ac.uk)
  • The proposed candidate is a universal cell therapy designed to generate new articular cartilage in these defects and interrupt the cycle of degeneration. (ca.gov)
  • Chondrocytes were isolated from articular cartilage of ten patients with primary OA undergoing knee replacement surgery and six normal donors undergoing fracture repair surgery without history of joint disease and no OA clinical manifestations. (biomedcentral.com)
  • Successful Fixation of Traumatic Articular Cartilage-Only Fragments in the Juvenile and Adolescent Knee: A Case Series. (mayo.edu)
  • [ 2 ] It can be thought of as primarily a degenerative disorder with inflammatory components arising from the biochemical breakdown of articular (hyaline) cartilage in the synovial joints. (medscape.com)
  • Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. (ca.gov)
  • Light microscopic findings indicated progressive damage with increasing duration of treatment and included, in various degrees of severity, clefts in articular cartilage containing collagen strands, pyknotic nuclei in necrotic chondrocytes, cartilaginous flaps, and vesicles. (cdc.gov)
  • Ultramicroscopic examination confirmed and characterized the degeneration and necrosis of the chondrocytes and demonstrated a decrease in density of collagen fibers in lesion areas along with fragmented fibers. (cdc.gov)
  • Cartilage sections were analyzed by immunohistochemistry for the presence of GrB, and the mRNA expression of GrB in chondrocytes was analyzed by in situ hybridization and nonquantitative and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). (elsevierpure.com)
  • In situ hybridization and RT-PCR confirmed the expression of GrB and PFN mRNA, and semiquantitative RT-PCR showed elevated concentrations of GrB and PFN expression in RA chondrocytes. (elsevierpure.com)
  • ASC (light grey bars), BMSC (white bars), mixed cultures (BMSC and chondrocytes in a ratio of 1:1, dark grey bars) and chondrocytes (black bars) were kept as monocultures (dotted lines at 100%) or as co- and tricultures (FB) with NB or OAB explants in chondrogenic medium. (biomedcentral.com)
  • Articular cartilage is an avascular tissue with low potential for self-repair. (nature.com)
  • Our lab's mission is to develop therapies for regenerating human tissues lost due to diseases or aging, and to build tissue engineered 3D models for understanding disease progression and informing drug discovery. (stanford.edu)
  • Just after induceJOURNAL OF EXTRACELLULAR VESICLESEVs release, the conditioned medium derived from pre-cultured with ADSCs had been isolated, then was utilised to deal with articular chondrocytes. (achrinhibitor.com)
  • CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFbeta and BMP through SMAD signaling. (ca.gov)
  • Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes. (ca.gov)
  • Results Cell viability was increased at lower concentrations of hydroxytyrosol in OA chondrocytes (P1, 30th day). (metu.edu.tr)
  • There may be no major difference in cell proliferation of chondrocytes among three groups. (achrinhibitor.com)
  • Besides the cell source choice, also mechanical stimuli are crucial in the development and maintenance of articular cartilage. (nature.com)
  • Strategies: Human ADSCs have been labelled with CM-DiI and after that pre-cultured in DMEM supplemented with 2 FBS for 48 h to induce EVs release. (achrinhibitor.com)
  • This study used etoposide, to induce DNA damage-related senescence or chronic exposure to IL-1β to entail inflammation-related senescence in human OA chondrocytes. (aging-us.com)
  • Chronic exposure to IL-1β induces only partial expression of senescence markers and does not allow us to conclude on its ability to induce senescence in chondrocytes. (aging-us.com)
  • Etoposide-induced senescence model may help investigate the initiation of cellular senescence in chondrocytes, and provide a useful model to develop therapeutic approaches to target senescence in OA. (aging-us.com)
  • These data confirm the metabolic heterogeneity of human chondrocytes, and suggest that NO may be involved to different degrees as an endogenous modulator of the turnover of the cartilaginous matrix in different zones of articular cartilage. (elsevierpure.com)
  • In summary, human cartilage grafts were successfully generated, culturing BM-MSCs with a relatively low fraction of non-expanded ACs in porous scaffolds. (unibas.ch)
  • H 2 O 2 oxidation of cysteine residues in c-Jun N-terminal kinase 2 (JNK2) contributes to redox regulation in human articular chondrocytes. (bvsalud.org)
  • However, both polyphenols significantly increased proliferation and reduced intracellular ROS generation in OA chondrocytes at lower concentrations. (metu.edu.tr)
  • Since the chondrocytes in adult human cartilage are normally quiescent and maintain the matrix in a low turnover state, understanding how they undergo phenotypic modulation and promote matrix destruction and abnormal repair in OA may lead to identification of critical targets for therapy to block cartilage damage and promote effective cartilage repair. (hss.edu)
  • Adult articular cartilage presents poor intrinsic capacity for regeneration, and after injury, cellular or biomaterial-based therapeutic platforms are required to assist repair promotion. (intechopen.com)
  • On the other hand, etoposide treatment reliably induces DNA damage-related senescence in human articular chondrocytes evidenced by loss of proliferative capacity, DNA damage accumulation, and expression of some SASP components. (aging-us.com)
  • In OA, while it has been shown to reduce the severity of the disease in the murine model [ 8 , 16 ], the senolytic UBX0101 failed to demonstrate clinical efficacy in humans. (aging-us.com)
  • These data demonstrate that MAP3K4-dependent signalling events are required for normal expression of Sry during testis development, and create a novel entry point into the molecular and cellular mechanisms underlying sex determination in mice and disorders of sexual development in humans. (plos.org)
  • Therefore, our study aimed to generate models of chondrocyte senescence. (aging-us.com)
  • Title : Objective Evaluation of Chondrocyte Density & Cloning after Joint Injury using Convolutional Neural Networks Personal Author(s) : Yang, Linjun;Martin, James A.;Brouillette, Marc J.;Buckwalter, Joseph A.;Goetz, Jessica E. (cdc.gov)