Human annexins. Medical search. Frequent questions

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. (nih.gov)
Anxa5 is a member of the annexin family of proteins, which consists of 12 structurally related, highly conserved proteins in humans and mice 4 . (nature.com)
This gene encodes a member of the annexin family. (novusbio.com)
Rabbit anti Human Annexin V antibody ( AHP1924 ) recognizes human annexin V (ANXA5), otherwise known as lipocortin V, a calcium-dependent phospholipid-binding protein and member of the annexin family, which binds with high affinity and selectivity to phosphatidylserine (PS). (bio-rad-antibodies.com)
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. (biolegend.com)

The annexins or lipocortins are a new family of calcium-dependent phospholipid-binding proteins. (nih.gov)
Annexins are a family of phospholipid-binding proteins with calcium ion-binding activity, which have many functions. (biomedcentral.com)
Annexins are a family of phospholipid-binding proteins with calcium ion-binding activity, which function in calcium signaling, and are widely distributed in eukaryotic cells [ 9 ]. (biomedcentral.com)
Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. (creativebiomart.net)

In flow experiment, annexin V is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane. (biofargo.com)
Jia L, Gu W, Zhang Y, Jiang B, Qiao X, Wen Y. Activated Yes-Associated Protein Accelerates Cell Cycle, Inhibits Apoptosis, and Delays Senescence in Human Periodontal Ligament Stem Cells. (medsci.org)
To provide insight into the biological effects of activated Yes-associated protein (YAP) on the proliferation, apoptosis, and senescence of human periodontal ligament stem cells (h-PDLSCs). (medsci.org)
Apoptosis was analyzed by Annexin V-APC staining. (medsci.org)
BP-induced apoptosis was demonstrated by Annexin V-FITC staining and TUNEL assay, while the expressions of apoptosis-related proteins were determined by western blot. (biomedcentral.com)
It was demonstrated that pemetrexed inhibited cell survival and induced G0/G1 cell cycle arrest and apoptosis in human ESCC cells. (spandidos-publications.com)
The efficacy of different dose groups (CM, CM5, CM10) was conducted on the proliferation, apoptosis, and α-smooth muscle actin (α-SMA) expression of human keloid fibroblasts (HKFs) in vitro. (biomedcentral.com)
Materials and Method: The roles of GM3 synthase (α2,3-sialyltransferase, ST3Gal V) in attenuating Taxol-induced apoptosis and triggering drug resistance were determined by cloning and overexpressing this enzyme in the SKOV3 human ovarian cancer cell line, treating SKOV3 and the transfectants (SKOV3/GS) with Taxol and determining apoptosis, cell survival, clonogenic ability, and caspase-3 activation. (scirp.org)
RPE viability was assessed by MTT and apoptosis was detected by Annexin-V via flow cytometry. (nih.gov)
Changes in cell viability, apoptosis, lactate dehydrogenase release, and cell cycle analysis were measured by cell counting kit-8 assay, annexin V/ propidium iodide staining, and enzyme-linked immunosorbent assay, respectively. (medsci.org)
Skills in cell cycle analysis using FACS and Annexin V assay for apoptosis analysis by FACS are also required. (nih.gov)
Harpagide up-regulates microRNA-140-5p to inhibit the oxidative stress and apoptosis of oxidized low-density lipoprotein-induced human umbilical vascular endothelial cells. (ijpsonline.com)
Apoptosis was measured by annexin V/PI dual staining. (bvsalud.org)
Apoptosis or necrosis was detected by measuring active Caspase 3/7 and 8, Bcl-2, Bax and Annexin V staining. (ucl.ac.uk)

The calcium-binding sites are required for annexins to aggregate and cooperatively bind anionic phospholipids and extracellular matrix proteins. (wikipedia.org)
Fusion also depended on cell-surface associated phosphatidylserine-binding proteins annexins (A1 and A5 for murine cells and A5 for human cells). (nih.gov)
Proteins from human lung adenocarcinoma cell line A549 lysates were subjected to extensive fractionation. (nih.gov)
Annexins constitute a family of calcium-dependent membrane-binding proteins and can be classified into two groups, depending on the length of the N-terminal domain unique for each individual annexin. (ed.ac.uk)

Annexin A5 (Anxa5) is a candidate autoantigen. (nature.com)

The anti-proliferative effect of BP on human bladder cancer cells was investigated and their cell cycle profiles after BP treatment were determined by flow cytometry. (biomedcentral.com)

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 monoclonal antibody (M02), clone 1G7 Analysis of ANXA2 expression in HepG2. (novusbio.com)
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of monoclonal antibody to ANXA2 on HeLa cell. (novusbio.com)
Annexin A2 (ANXA2) is a pro-metastasis protein, previously identified as a relevant PDAC antigen that is expressed by a GM-CSF-secreting allogenic whole pancreatic tumor cell vaccine (GVAX) to induce an anti-ANXA2 antibody response in patients with PDAC. (biomedcentral.com)

No. 305704) for 4 hours at 37°C, then stained with Annexin V- Pacific Blue™ for 15 minutes at 37°C in Annexin V Binding buffer. (biolegend.com)
Annexin V Binding Buffer (cat. (biolegend.com)
For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). (biolegend.com)
No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. (biolegend.com)
6. Add 400 µL of Annexin V Binding Buffer (Cat. (biolegend.com)

no. 422201) is recommended for use with Annexin V staining. (biolegend.com)
As demonstrated by assays of LDH release, dead red staining and annexin V staining, we found these QDs to be minimally toxic to exposed macrophages. (cdc.gov)
Cell death was assessed by dual staining with Annexin-V-fluorescein isothiocyanate (FITC) and propidium iodide (PI), which allows the discrimination of viable (FITC-/PI-), apoptotic (FITC+/PI-) and necrotic cells (FITC-F/PI+). (boku.ac.at)

Human peripheral blood lymphocytes were stained with CD19 (clone SJ25C1) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram). (biolegend.com)

The annexins are characterised by their related structures, which are composed of four repeats (eight for annexin A6) of approximately 60 amino acids 13 that allow calcium-dependent binding to phospholipid membranes. (nature.com)
The annexin VI gene is approximately 60 kbp long and contains 26 exons. (creativebiomart.net)

The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus, recurrent spontaneous abortions and systemic sclerosis. (bio-rad-antibodies.com)

Recombinant Human ANXA6 Protein 1-673 aa (full-length) with a 6xHis tag was expressed in E.coli. (creativebiomart.net)

Annexin III has been previously identified as inositol 1,2-cyclic phosphate 2-phosphohydrolase (EC 3.1.4.36), an enzyme of inositol phosphate metabolism, and also as placental anticoagulant protein III, lipocortin III, calcimedin 35-alpha, and an abundant neutrophil cytoplasmic protein. (nih.gov)
Chromosomal localization of the human gene for annexin V (placental anticoagulant protein I) to 4q26----q28. (nih.gov)
Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. (bio-rad-antibodies.com)

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - MDA-MB-231 subclones whole cell lysates were loaded with 30 ug/lane. (novusbio.com)

abstract = "Annexin I in the cytosol of resting neutrophils was translocated to the plasma membranes upon addition of opsonized zymosan (OZ). Maximum translocation could be detected 1 min after stimulation with OZ, and decreased thereafter. (bgu.ac.il)

Results from transcriptional profiling during development show that annexin B22 expression is correlated with life stages of the parasite that possess the syncytial tegument layer, and ultrastructural localization by immuno-electron microscopy confirms the occurrence of annexins in the tegument of S. mansoni. (rcsb.org)
Immunofluorescence imaging of HaCaT cells shows an IFNgamma-dependent co-localization of annexin II with Pyk2 in the perinuclear region, suggesting that annexin II mediates the calcium-dependent regulation of Pyk2. (nih.gov)

Methods: Human ARPE-19 and primary mouse RPE were incubated with lipopolysaccharide (LPS), 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), or H2O2. (nih.gov)

Data from membrane binding and aggregation assays indicate the presence of differential molecular mechanisms and support the hypothesis of annexin B22 providing structural integrity in the tegument. (rcsb.org)
The migration inhibitory effect of BP on human bladder cancer cells were shown by trans-well and wound healing assays. (biomedcentral.com)

Here, we present the first structural and immunobiochemical characterization of an annexin from Schistosoma mansoni. (rcsb.org)

Annexin V has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. (biofargo.com)
The binding of peptides to membranes was confirmed by surface pressure (Langmuir film balance) measurements using phosphatidylcholine/phosphatidylserine monolayers, which show a significant increase after injection of rat annexin A1 N-terminal peptides. (ed.ac.uk)

This gene encodes a divergent member of the annexin protein family in which all four homologous type II calcium-binding sites in the conserved tetrad core contain amino acid substitutions that ablate their function. (wikipedia.org)

It is highly similar to human annexins I and II sequences, each of which contain four such repeats. (creativebiomart.net)

7. Tumor-Suppressing Effect of Silencing of Annexin A3 Expression in Breast Cancer. (nih.gov)
Taxol treatment upregulated the expression of human GM3 synthase, an enzyme that transfers a sialic acid to lactosylceramide. (scirp.org)
Applied Biosystems' high-resolution Human Transcriptome Array 2.0 was used to examine gene expression. (bvsalud.org)

2. Downregulation of annexin A3 inhibits tumor metastasis and decreases drug resistance in breast cancer. (nih.gov)
Different doses (20, 40, 80 μmol/l) of harpagide were applied to treat human umbilical vascular endothelial cells induced by oxidized low-density lipoprotein, human umbilical vascular endothelial cells overexpressing microRNA-140-5p were induced by oxidized low-density lipoprotein, and 80 μg/ml harpagide was applied to treat oxidized low-density lipoprotein induced human umbilical vascular endothelial cells with microRNA-140-5p downregulation. (ijpsonline.com)

TGF-β is a very potent growth inhibitor of primary human mammary epithelial cells. (springer.com)

Annexin A9 is a protein that in humans is encoded by the ANXA9 gene. (wikipedia.org)
Human ANXA9 genome location and ANXA9 gene details page in the UCSC Genome Browser. (wikipedia.org)

Two annexin protein family genes [ Echinococcus granulosus annexin B3 ( Eg AnxB3) and Eg AnxB38] were cloned and molecularly characterized using bioinformatic analysis. (biomedcentral.com)

Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. (creativebiomart.net)
The N-terminal domain of annexin A1 can adopt an alpha-helical conformation and has been implicated in mediating the membrane aggregation behavior of this protein. (ed.ac.uk)
The results are consistent with the hypothesis that the N-terminal domain of annexin A1 can serve as a secondary membrane binding site in the process of membrane aggregation by providing a peripheral membrane anchor. (ed.ac.uk)

Chromosomal mapping of the human annexin IV (ANX4) gene. (nih.gov)

Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. (biolegend.com)
Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. (biolegend.com)

In this study, the gene (ANX3) encoding annexin III was localized to human chromosome 4 at band q21 (q13-q22) by (1) polymerase chain reaction analysis of a human-rodent hybrid cell panel, confirmed by genomic Southern blot analysis of the same panel with a cDNA probe and (2) in situ hybridization with a cDNA probe. (nih.gov)
Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death. (bio-rad-antibodies.com)
Human T leukemia cell line Jurkat, treated (left) or non-treated (right) with BioLegend's anti-human CD95 (EOS9.1) mAb (Cat. (biolegend.com)
From sourcing your starting sample to downstream B cell analysis, explore our complete portfolio of products to support your human B cell research workflow. (stemcell.com)
Unsure which human B cell isolation product is right for your research? (stemcell.com)
10. Effects of TGF-β signaling blockade on human A549 lung adenocarcinoma cell lines. (nih.gov)
17. Suppression of cell invasion and migration by propofol are involved in down-regulating matrix metalloproteinase-2 and p38 MAPK signaling in A549 human lung adenocarcinoma epithelial cells. (nih.gov)
h-PDLSCs were isolated successfully and were positive for human mesenchymal stem cell surface markers. (medsci.org)
The cause of penile squamous cell carcinoma (SCC) is unclear, although human papillomavirus (HPV) appears to play a major role in many cases. (medscape.com)
Human spinal cord cell atlas provides foundation to study neurodegeneration, chronic pain, and other diseases. (nih.gov)
The NIH Brain Research Through Advancing Innovative Neurotechnologies® (BRAIN) Initiative Cell Census Network (BICCN) has unveiled an atlas of cell types and an anatomical neuronal wiring diagram for the mammalian primary motor cortex, derived from detailed studies of mice, monkeys, and humans. (nih.gov)
Using advanced live cell microscopy, the researchers found that annexin A11 plays a role in this process by helping RNA granules hitch rides onto traveling lysosomes. (nih.gov)
By testing approaches and technologies in cell lines and animal models, TFGB investigators are making fundamental discoveries that will be critical to the success of human clinical trials. (nih.gov)
Experimental work evaluated the measurement of M2-pyruvate kinase in human pancreatic cancer cell lines with altered microenvironment (hypoxia, acidic pH or glucose-deprived condition). (ucl.ac.uk)

Occurrence of autoantibodies to annexin I, 14-3-3 theta and LAMR1 in prediagnostic lung cancer sera. (nih.gov)
We present evidence for the occurrence in lung cancer sera of autoantibodies to annexin I, 14-3-3 theta, and a novel lung cancer antigen, LAMR1, which precede onset of symptoms and diagnosis. (nih.gov)

Cultured human umbilical vein endothelial cells (HUVECs) were exposed to H 2 O 2 as oxidative stress and a high glucose concentration with palmitate as a glucolipotoxic condition. (medsci.org)
on human umbilical vascular endothelial cells damage induced by oxidized low-density lipoprotein and its possible mechanism. (ijpsonline.com)
Human umbilical vascular endothelial cells were cultured in vitro. (ijpsonline.com)
Detection of Swt1 (pink), a protein that localizes in the nuclear envelope, and mitochondria (yellow) in human glomerular microvascular endothelial cells (HGMECs). (nih.gov)

Recombinant annexin B30 of S . mansoni gave no significant protection against the parasite, which suggested it may not be suitable as a vaccine candidate [ 21 ]. (biomedcentral.com)

Nickel is harmful for humans, but molecular mechanisms of its toxicity are far from being fully elucidated. (waw.pl)
Altogether, our results indicate that human annexins A1, A2, and A8 are potential molecular targets for nickel toxicity and help design appropriate cellular studies. (waw.pl)

This study used circular dichroism spectroscopy to show that a rat annexin A1 N-terminal peptide possesses random coil structure in aqueous buffer but an alpha-helical structure in the presence of small unilamellar vesicles. (ed.ac.uk)

RÉSUMÉ Pour étudier les effets de la silymarine sur le développement folliculaire, nous avons recruté 40 femmes en bonne santé subissant une fécondation in vitro (FIV) en raison d'une infertilité masculine. (who.int)

Members of the annexin family contain 4 internal repeat domains, each of which includes a type II calcium-binding site. (wikipedia.org)
Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. (creativebiomart.net)
It was reported that the mRNA and activity levels of a member of the ST family, α2,3-sialyltransferase ST3Gal V (GM3 synthase) [13], positively correlated with resistance to the synthetic retinoid N-(4-hydroxyphenyl)-retinamide [14] in A2780 human ovarian carcinoma cells. (scirp.org)

Annexins are thought to be of integral importance for the stability of this apical membrane system. (rcsb.org)
Although the calcium-independent interaction of the annexin A1 N-terminal domain has been known for some time, there was no structural information about the membrane interaction of this secondary membrane-binding site of annexin A1. (ed.ac.uk)
Lamellar neutron diffraction with human and rat annexin A1 N-terminal peptides reveals an intercalation of the helical peptides with the phospholipid bilayer, with the helix axis lying parallel to the surface of membrane. (ed.ac.uk)

We used freshly isolated human erythrocytes and ex vivo and in vivo thrombosis models in rats to investigate mercury-induced procoagulant activity. (nih.gov)

Proficiency in cytogenetic techniques is essential, including whole blood culture for metaphase chromosome preparation, G-banding, C-banding, and karyotyping of human chromosomes. (nih.gov)

3. Annexin A3 depletion overcomes resistance to oxaliplatin in colorectal cancer via the MAPK signaling pathway. (nih.gov)

Human monocyte-derived macrophages internalized the submicron-sized Ru(bpy)32+-zeolite crystals, and intracellular oxygen concentrations initiated by zymosan-mediated oxidative burst could be monitored by measuring the emission from Ru(bpy)32+ by confocal fluorescence microscopy. (cdc.gov)

9. Hsa-miR-623 suppresses tumor progression in human lung adenocarcinoma. (nih.gov)
Tumor model in NOD-SCID mice were induced by injection of BFTC human bladder cancer cells. (biomedcentral.com)

Subcellular fractionation studies demonstrated that annexin I could not be detected in the granule fractions in either resting or activated cells, but was found in association with the phagosome fraction. (bgu.ac.il)
Annexin I is not phosphorylated in resting or stimulated cells. (bgu.ac.il)
In this study, we developed new FRET biosensors for dual imaging of intracellular ATP level and caspase-3 activity in single apoptotic cultured human cells. (elifesciences.org)
Deoxynivalenol is known to affect the proliferation and viability of animal and human intestinal epithelial cells. (boku.ac.at)
7-Methylxanthine Influences the Behavior of ADORA2A-DRD2 Heterodimers in Human Retinal Pigment Epithelial Cells. (illumina.com)

In particular, studies performed by our group led to the description of a characteristic proteomic profile of circulating human monocytes in acute coronary syndromes. (medscape.com)

The crystal structure of annexin B22 confirms the presence of the previously predicted α-helical segment in the II/III linker and reveals a covalently linked head-to-head dimer. (rcsb.org)
6. Quantitative proteome analysis reveals annexin A3 as a novel biomarker in lung adenocarcinoma. (nih.gov)

Basic research increases our understanding of human behavior and biology, which is foundational to advancing new and better ways to prevent, diagnose, and treat disease. (nih.gov)

The overexpression of Human Epidermal Growth Factor Receptor 2 (HER2) is usually associated with aggressive and infiltrating breast cancer (BC) phenotype, and metastases. (dovepress.com)

Anatomy1

Organisms2

Chemicals and Drugs13

Member of the annexin family5

Phospholipid-binding4

Apoptosis14

Proteins4

Anxa51

Flow cytometry1

ANXA23

Binding Buffer5

Staining3

Isotype1

Approximately 602

Antibodies1

ANXA61

Anticoagulant protein3

Western Blot1

Abstract1

Localization2

Methods1

Assays2

Schistosoma1

Phosphatidylserine2

Gene encodes1

Sequences1

Expression3

Downregulation2

Inhibitor1

ANXA92

Protein family1

Aggregation3

ANX41

Apoptotic cells2

Cell14

Occurrence2

Endothelial cells4

Mansoni1

Molecular2

Peptide1

Vitro1

Family3

Membrane3

Thrombosis1

Chromosome1

Colorectal cancer1

Microscopy1

Tumor2

Cells5

Description1

Reveals2

Behavior1

Breast Cancer1