• Les IgIV intéragissent avec de nombreux composants du système immunitaire comme les récepteurs Fc, le complément, les cytokines, les lymphocytes T et B, les cellules dendritiques, les granulocytes et les cellules NK, ce qui explique en partie leurs effets anti-inflammatoires. (academie-medecine.fr)
  • 2 The interaction of IVIG with a large number of components of the immune system including Fc receptors, complement molecules, cytokines, B and T lymphocytes, neutrophils and NK cells, may explain at least in part their anti-inflammatory effects. (academie-medecine.fr)
  • It inhibited the induction of pro-inflammatory cytokines in both cell types by targeting the NFκB signal transduction pathways in a mechanism that may involve the upregulation of SOCS1 and SOCS3. (dcu.ie)
  • This triggers the formation of ASC-GFP specks, the release of cytokines (eg IL-16 and IL-18) and pyroptotic cell death. (invivogen.com)
  • Innate lymphoid cells (ILCs) lack antigen receptors but are equipped with receptors for cytokines, hormones, and paracrine signals enabling them to sense their environment and respond quickly by producing factors that influence parenchymal cells and other resident immune cells. (elifesciences.org)
  • The α7 nicotinic acetylcholine receptor (α7 nAChR) negatively regulates the synthesis and release of pro-inflammatory cytokines by immune cells. (listlabs.com)
  • The treatment reduced encephalitogenic T cell proliferation and the production of pro-inflammatory cytokines, as well as increased the production of the anti-inflammatory cytokine IL-10. (listlabs.com)
  • Different subpopulations of immune cells interplay to directly or indirectly regulate other subpopulations by releasing different cytokines and other mediators of inflammation, thereby contributing to the progression of the systemic disease. (biomedcentral.com)
  • Results: In vitro, Rapa-mDCs exhibit a semi-mature phenotype given by intermediate levels of co-stimulatory molecules and cytokines, and inhibit CD4 + T-cell proliferation. (uandes.cl)
  • Finally, a number of biologics that target CD4 + T cell differentiation into inflammatory subsets or their byproducts (i.e., cytokines) have shown efficacy in treating patients with IBD ( 5 - 7 ). (frontiersin.org)
  • Given the importance of CD4 + Th cells in the disease process, this review will focus on how Th cells differentiate in the inflamed intestinal tract during IBD and how the Th lineage-specific cytokines and transcription factors (TFs) contribute to disease. (frontiersin.org)
  • Interleukin (IL)-12 and IL-23, cytokines induced during early stages of IBD, play important roles in differentiation of interferon (IFN)-γ/tumor necrosis factor (TNF)-producing Th1 cells as well as IL-17-producing Th17 cells. (frontiersin.org)
  • The culture supernatants of all of the strains exerted inhibitory effects on the growth of E. coli , Salmonella , Listeria and Yersinia , and a variable, strain-dependent induction was observed of both pro- and anti-inflammatory cytokines in human DCs. (biomedcentral.com)
  • Continuous ligation of LILRB1 modulated cellular differentiation, conferred a unique phenotype upon the resultant cells, induced a profound resistance to CD95-mediated cell death, and inhibited secretion of cytokines IL-10, IL-12p70, and TGF-β. (ashpublications.org)
  • Natural-killer (NK) cells are specialized lymphocytes that have cytotoxic properties in addition to their ability to produce cytokines that assist in the orchestration of adaptive immunity. (medscape.com)
  • 0.5 IU of virus-neutralizing antibodies after administration), vaccine matching, seroconversion testing with an ELISA after completion of postexposure prophylaxis (PEP) in humans, virus-neutralizing antibody titration in vaccinated animals because of inferior vaccines, and other related issues. (cdc.gov)
  • Continuous cultures of fused cells secreting antibody of predefined specificity. (scriptiebank.be)
  • Goat Anti-Mouse IgG 2a -HRP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blot, and ELISpot assays. (southernbiotech.com)
  • Each cell line was incubated with Anti-ACE2-PE antibody for 1 hour at 4°C. Cell surface staining was analyzed by flow cytometry. (invivogen.com)
  • Lysates from A549-ASCoV2 (1) and A549-ASCoV2-NLRP1 (2) cells were analyzed by western blot using an anti‑human NLRP1 and an anti-human ASC antibody, followed by HRP conjugated secondary antibody. (invivogen.com)
  • The development of new methods to predict the protein reactive potential of sensitizing substances indicated that such compounds are able to react with proteins on the cell surface and that this reaction impacts the detection of such proteins by antibody staining. (tu-darmstadt.de)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • Although these studies warrant independent confirmation, they support the notion that apoptotic cells could harbor the immunogens responsible for antinuclear antibody formation. (biomedcentral.com)
  • The aim of the project was to investigate how CD11c is distributed in the blood, how 2 CD11c antibody clones affect dendritic cell maturation and if they are internalized by the dendritic cells. (aau.dk)
  • Neither of the CD11c antibody clones induced maturation of dendritic cells. (aau.dk)
  • PE conjugated Mouse anti Human CD25 antibody, clone MEM-181 ( MCA2127PE ) used for the identification of human Tregs by flow cytometry. (bio-rad-antibodies.com)
  • NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)
  • However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)
  • Background: Tumor-specific cytotoxic T lymphocytes (CTL) can be activated in vivo by vaccination with dendritic cells (DC). (uni-muenchen.de)
  • In the context of cancer, appropriately activated DCs can induce anti-tumor immunity by activating innate immune cells and tumor-specific lymphocytes that target cancer cells. (mdpi.com)
  • In addition, diabetic mice presented a reduced influx of TCD4+ cells, TCD8+ cells, B lymphocytes, NK cells, and dendritic cells compared to control infected groups. (hindawi.com)
  • Insulin treatment restored the leukocyte number in PeLF and restored the presence of B lymphocytes, dendritic cells, and NK cells in lungs of diabetic animals. (hindawi.com)
  • The data suggest that diabetic mice present impaired immunological response to Pb18 infection and insulin modulates inflammation by reducing IL-6 levels in lung and CINC-1 levels in spleen and liver homogenates, restoring leukocyte concentrations in PeLF and also restoring populations of dendritic cells and B lymphocytes in lungs of diabetic mice, permitting the host to better control the infection. (hindawi.com)
  • Paradoxically, while we have known for a long time the key role played by these cells in the activation of B and T lymphocytes, and we identified the sensors that allow them to detect danger signals emitted by pathogens, operation of this interface remains partly a mystery. (univ-mrs.fr)
  • To deliver these immunological instructions, antigen (in practice a peptide a few amino acids in length) is not presented in isolation to T lymphocytes by dendritic cells but is nestled in a pocket formed by a molecule, known as the Major Histocompatibility Complex (called HLA in humans). (univ-mrs.fr)
  • CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. (thermofisher.com)
  • CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes. (thermofisher.com)
  • The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
  • Macrophages and dendritic cells are usually the first point of contact with pathogens, including lentiviruses. (wikipedia.org)
  • Once inhaled by the host, the fungus interacts with alveolar macrophages and dendritic cells present in the lungs, where it can form a pulmonary focus or spread through the blood and lymphatic vessels [ 6 ]. (hindawi.com)
  • Macrophages and dendritic cells are both key players in the induction, propagation and resolution of inflammatory responses, and are known to actively contribute to the pathogenesis of many inflammatory diseases. (dcu.ie)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • For prophylactic vaccination, mice were vaccinated three times in weekly intervals prior to tumor challenge with Panc02 cells. (uni-muenchen.de)
  • In contrast, neither knockout nor overexpression of PD-L1 in tumor cells had an effect on PD-L1 blockade efficacy. (nih.gov)
  • Human and murine studies showed high levels of functional PD-L1 expression in dendritic cells and macrophages in the tumor microenvironments and draining lymph nodes. (nih.gov)
  • Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. (ancell.com)
  • We here determined using CMV as a vaccine platform whether critical thresholds of vaccine-specific T cell responses can be established that relate to tumor protection, and which factors control such thresholds. (bmj.com)
  • The magnitude, kinetics and phenotype of the circulating tumor-specific CD8 + T cell response were determined. (bmj.com)
  • Mice were subsequently challenged with tumor cells, and the tumor protection was monitored. (bmj.com)
  • 0.3% of the total circulating CD8 T cell population fully protects mice against lethal tumor challenge. (bmj.com)
  • However, low dose inoculations via the IP or SC route or IN vaccination elicited vaccine-induced CD8 + T cell responses that did not reach protective thresholds for tumor protection. (bmj.com)
  • In addition, whereas weak pre-existing immunity did not alter the protective thresholds of the vaccine-specific T cell response following subsequent immunization with CMV-based vaccine vectors, strong pre-existing immunity inhibited the development of vaccine-induced T cells and their control on tumor progression. (bmj.com)
  • Conclusions This study highlight the effectiveness of CMV-based vaccine vectors, and shows that demarcated thresholds of vaccine-specific T cells could be defined that correlate to tumor protection. (bmj.com)
  • These cells primarily produce interferon (IFN)-γ and tumor necrosis factor (TNF) that, respectively, activate macrophages and direct cytotoxic CD8 + T cell responses, that in turn promote elimination of intracellular pathogens such as viruses and bacteria ( 8 ). (frontiersin.org)
  • This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). (jove.com)
  • The overall goal of this study is to compare two different methods of human monocyte isolation for dendritic cell generation in vitro and to characterize the CT11c, CT14 cell surface expression of the resulting monocyte derived populations by imaging flow cytometry. (jove.com)
  • We report that SWCNT coating with a phospholipid ''eat-me'' signal, phosphatidylserine (PS), makes them recognizable in vitro by different phagocytic cells - murine RAW264.7 macrophages, primary monocyte-derived human macrophages, dendritic cells, and rat brain microglia. (cdc.gov)
  • All kappa-casein induced phenotypes significantly suppressed the production of IL-2 from CD4+ T-cell in-vitro & in in-vivo, a key cytokine required for effector T-cell responses. (dcu.ie)
  • More recently, ontogenetic, transcriptional, and proteomic research approaches uncovered major developmental differences between Flt3L-dependent conventional DCs as compared with Mphs and monocyte-derived DCs (MoDCs), the latter mainly generated in vitro from murine bone marrow-derived DCs (BM-DCs) or human CD14\(^{+}\) peripheral blood monocytes. (uni-wuerzburg.de)
  • Conversely, in vitro GM-CSF-dependent monocyte-derived Mphs largely resemble MoDCs whereas tissue-resident Mphs show a common embryonic origin from yolk sac and fetal liver with Langerhans cells (LCs). (uni-wuerzburg.de)
  • Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)
  • The subunits are held together and connected to the underlying cell surface by non-covalent interactions, and they spontaneously reassemble in vitro by an entropy-driven process, i.e. the subunit proteins are very poorly water-soluble [ 8 ]. (biomedcentral.com)
  • Taken together, our results indicate that LV vectors can serve to design pDC-based vaccines in humans, and they are also useful in vitro to evaluate the immunogenicity of the vector preparations, and the specificity and safety of given promoters used in gene therapy protocols. (biomedcentral.com)
  • These in vitro, and their expression in these human tumour virus. (who.int)
  • Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. (southernbiotech.com)
  • To investigate the role of SDF-1 in recirculation and homing in vivo we have developed a model in which human peripheral lymph nodes (huPLN) are transplanted into SCID mice. (biomedcentral.com)
  • This is the first description of huPLN transplantation into SCID mice, and of the functional effects of SDF-1 regarding the migration of human cells into huPLN in vivo . (biomedcentral.com)
  • Here, we evaluate a potential protocol using a murine in vivo transplant model. (uandes.cl)
  • Moreover, The use of animals as surrogate rine host, can provide a platform for animal models for tumour viruses in hosts for the study of human tu- in vivo infection. (who.int)
  • For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)
  • These monocytes isolation protocols can contribute to the development of reliable methods for the purification of human dendritic cells for research and clinical applications. (jove.com)
  • The main advantage of these protocols is that they facilitate the isolation of human monocytes and their differentiation into viable and functional monocyte derived cells. (jove.com)
  • To isolate monocytes by the adherence method, culture five times 10 to the seven cells of the resulting PBMC pellet per 10 milliliters of complete medium in a new T75 flask for two hours at 37 degrees celsius and five percent carbon dioxide in a humidified incubator. (jove.com)
  • Results showed that CD11c wasexpressed by both dendritic cells and monocytes in the blood. (aau.dk)
  • Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)
  • The human Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species including plant Hsp60 (known as Rubisco binding protein), and bacterial GroEL, a major antigen of mycobacteria. (enzolifesciences.com)
  • Interleukin (IL)-12, which is secreted by antigen-presenting cells, acts via signal transducer and activator of transcription (STAT)4 to promote the differentiation of naïve T cells into Th1 cells ( 9 - 11 ). (frontiersin.org)
  • T helper cells recognize antigen presented in the context of major histocompatibility complex II on antigen-presenting cells in a T cell receptor-dependent fashion (not shown). (frontiersin.org)
  • In addition to the direct cytotoxic effect on tumour cells, dolastatins efficiently promoted antigen uptake and migration of tumour-resident DCs to tumour-draining lymph nodes. (biomedcentral.com)
  • Dendritic cells (DCs) are professional antigen-presenting cells that have multiple subpopulations with different phenotypes and immune functions. (biomedcentral.com)
  • Finally, mononuclear cells isolated from lung (L-MCs), which are used as precursors for L-DCs, expressed more antigen-presenting cell-associated markers such as MHC class II and CD172α compared to their counterparts from blood. (biomedcentral.com)
  • Dendritic cells (DCs) are the most important antigen-presenting cells (APCs) in the body. (biomedcentral.com)
  • Gene modified dendritic cells (DC) are able to modulate DC functions and induce therapeutic immunity or tolerance in an antigen-specific manner. (biomedcentral.com)
  • Dendritic cells have been studied intensely for a few decades for their excellent antigen-presenting capabilities, and lately the focus has been on the idea of utilizing them for therapeutic cancer vaccines. (aau.dk)
  • Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
  • CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (3). (ancell.com)
  • Figure A. Alexa®647 conjugated mouse anti human CD4 ( MCA1267A647 ) and RPE conjugated Mouse IgG1 isotype control ( MCA928PE ). (bio-rad-antibodies.com)
  • In this sequence of events, the team of Philippe Pierre and Evelina Gatti is particularly interested in the key stage of maturation of dendritic cells. (univ-mrs.fr)
  • We found that efficient gene transfer into pDC can be achieved with VSVG-pseudotyped lentiviral vectors (LV) under the control of phoshoglycerate kinase (PGK) and elongation factor-1 (EF1α) promoters (28% to 90% of E-GFP + cells, respectively) in the absence of phenotypic and functional maturation. (biomedcentral.com)
  • CD11c distribution was analyzed by flow cytometry, dendritic cell maturation by flow cytometry and microscopy and CD11c internalization by confocal microscopy and flow cytometry. (aau.dk)
  • Although defects may occur in many steps in B-cell development and maturation, resulting in agammaglobulinemia, the most common and well-described defect is the impaired maturation of the pro-B cells to pre-B cells. (medscape.com)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • 2 Previous studies have evaluated the opioid receptors on a wide array of immune cells. (uspharmacist.com)
  • Our team was also able to show that brain and DC-associated LAMP associated molecule (BAD-LAMP, C20orf103, LAMP5) is a chaperone for endocytic toll-like receptors (TLR) specifically expressed in the human type-I Interferon producing plasmacytoïd dendritic cells, which are specialized in the detection of nucleic acids potentially of viral or bacterial origin. (univ-mrs.fr)
  • A549-ASCoV2 NLRP1 cells and their control cell line overexpress the genes encoding the SARS-CoV-2 human receptors, ACE2 and TMPRSS2 [1], and are thus highly permissive to SARS-CoV-2 infection. (invivogen.com)
  • In this study, we investigated the expression of serotoninergic receptors (5-HTR), the signaling pathway, and biological activity of 5-HT on human dendritic cells (DC), showing that immature and mature DC expressed mRNA for different serotoninergic receptors. (aai.org)
  • 1 Activation of DCs through toll-like receptors (TLRs) for "infectious nonself" or other "danger" signals normally initiates a process of cellular differentiation resulting in "mature" DCs capable of stimulating T-cell and natural killer (NK)-cell responses. (ashpublications.org)
  • Recent studies have suggested a role for human leukocyte Ig-like receptors (LILRs, also known as ILT, LIR, or CD85) in regulating the function of myeloid cells, potentially implicating these molecules in the control of immune responses. (ashpublications.org)
  • The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
  • Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
  • Loss of USP18 results in increased responsiveness to type I interferons and life-threatening autoinflammatory disease in humans due to the negative regulatory function of USP18 in interferon signal transduction. (wikipedia.org)
  • To achieve a proper separation of the peripheral blood mononuclear cells, load the blood onto the density gradient solution quickly, but carefully and without mixing the layers. (jove.com)
  • Lymphoid lineage DCs primarily differentiate into plasmacytoid DCs and occupy approximately 0.5% of peripheral blood mononuclear cells (PBMCs) in humans [ 2 ], but the cell population percentage is unclear in horses. (biomedcentral.com)
  • Finally, co-incubation of human peripheral blood mononuclear cells with mitochondria-treated ECs results in increased numbers of effector (IFN-γ+ , TNF-α+ ) CD8+ T cells. (duke.edu)
  • Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
  • CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. (ancell.com)
  • While no clinical data establishes causal link between HIV acquisition and use of this injectable progestin, prior work from our laboratory showed that DMPA comparably lowers genital levels of the cell-cell adhesion molecule desmoglein-1 (DSG1) and weakens genital epithelial barrier function in female mice and humans. (stanford.edu)
  • Indeed, the NLRP1 sensor is activated upon cleavage by the SARS-CoV-2 3CL protease NSP5 in lung epithelial cells [2]. (invivogen.com)
  • Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells. (invivogen.com)
  • We demonstrated adhesive and significant pathogen inhibitory efficacies among the swine intestinal L. amylovorus strains studied, pointing to their potential use as probiotic feed supplements, but no independent role could be demonstrated for the major S-layer proteins in adherence to epithelial cells. (biomedcentral.com)
  • The results indicate that many intestinal bacteria may coexist with and confer benefits to the host by mechanisms not attributable to adhesion to epithelial cells or mucus. (biomedcentral.com)
  • Dendritic cells (DCs) and macrophages (Mph) share many characteristics as components of the innate immune system. (uni-wuerzburg.de)
  • More recently, ontogenetic, transcriptional, and proteomic research approaches uncovered major developmental differences between Flt3L-dependent conventional DCs as compared with Mphs and monocyte-derived DCs (MoDCs), the latter mainly generated in Dendritic cells (DCs) and macrophages (Mph) share many characteristics as components of the innate immune system. (uni-wuerzburg.de)
  • Determined by its ability to chemoattract using human peripheral blood eosinophils using a concentration of 50.0-100.0 ng/ml. (peprotech.com)
  • The neurotransmitter 5-hydroxytryptamine (5-HT), commonly known as serotonin, is stored at peripheral sites in mast cells and released from this peripheral source upon IgE cross-linking. (aai.org)
  • These similarities are supported by studies where the single-ring human mitochondrial homolog, Hsp60 with its cochaperonin, Hsp10 were expressed in an E. coli strain, engineered so that the GroE operon is under strict regulatory control. (enzolifesciences.com)
  • The mature protein, which also includes a C-terminal truncation, contains 78 amino acid residues (92 amino acid residues for the murine homolog, without C-terminal truncation). (peprotech.com)
  • Establishment and validation of the methods was performed with 54 test substances of known sensitizing potential, and the findings were compared to available human patch test data and murine local lymph node assay data. (tu-darmstadt.de)
  • Stromal cell derived factor-1 (CXCL12) induces cell migration into lymph nodes transplanted into SCID MICE. (biomedcentral.com)
  • Logically, the team is interested in finding out whether and by what means the dendritic cell redirects the transport of MHC molecules depending on the nature of microbial products that it has detected. (univ-mrs.fr)
  • By looking under the microscope at the behavior of MHC molecules in dendritic cells we found that the addition of microbial products induces dramatic changes in all cells in our culture," says Evelina Gatti, co-leader on this team subject. (univ-mrs.fr)
  • Initially on the inside of the cells, MHC molecules were suddenly exposed on the outside of the cell. (univ-mrs.fr)
  • We tried to understand how the dendritic cell organized trafficking of MHC molecules. (univ-mrs.fr)
  • Dendritic cell activation (MHC II molecules in green, Lysosomes in red, nuclei in grey). (univ-mrs.fr)
  • This allows the molecules to have a "ticket" for restricted access into specialized compartments of the cell. (univ-mrs.fr)
  • Change in the distribution of MHC class II molecules within a dendritic cell in the presence (top) or absence (bottom) of the ubiquitin ligase MARCH1. (univ-mrs.fr)
  • It is increasingly recognized that endogenous molecules released from tissue injury and cell death are potent activators of innate immunity. (duke.edu)
  • Additionally, mitochondrial exposure causes dendritic cells to upregulate costimulatory molecules. (duke.edu)
  • CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)
  • In the blood, mucous membranes and lymphoid organs, dendritic cells provide a dual role as sentinels, but also as conductors of the immune orchestra. (univ-mrs.fr)
  • This is the moment when the cells detect the microbes or danger, change their biological functions and begin their migration to secondary lymphoid organs. (univ-mrs.fr)
  • This model provides a powerful tool to investigate the pathways involved in cell-migration into lymphoid organs and potentially to target them for therapeutic purposes. (biomedcentral.com)
  • Additionally, GAT107 was found to directly activate α7 nAChR in murine macrophage RAW264.7 cells and in human PBMCs derived from MS patients and healthy donors. (listlabs.com)
  • Morphine has been shown to negatively impact macrophage function by impairing the proliferation of macrophage progenitor cells, inhibiting cytokine secretion leading to decreased chemotaxis, hindering the ability of macrophages to directly phagocytize pathogens, and inhibiting nitric oxide production. (uspharmacist.com)
  • Aim: To date, there is no human dendritic cell (DC) based therapy to prevent allograft rejection in transplanted patients. (uandes.cl)
  • Infusion of isolated mitochondria into heart donors leads to significant increase in allograft rejection in a murine heterotopic heart transplantation model. (duke.edu)
  • Background The capacity of cytomegalovirus (CMV) to elicit long-lasting strong T cell responses, and the ability to engineer the genome of this DNA virus positions CMV-based vaccine vectors highly suitable as a cancer vaccine platform. (bmj.com)
  • A recent study reported that oral antibiotic treatment caused dysbiosis of the vaginal microbiota, resulting in increased IL-33 secretion, leading to impaired antiviral T cell responses and enhanced susceptibility to lethal HSV2 infection 23 . (nature.com)
  • However, analogous effects of NET-EN treatment on antivirus immunity and host susceptibility to genital infection are much less explored. (stanford.edu)
  • Group 2 innate lymphoid cells (ILC2s) adapt to tissue physiology and contribute to immunity, inflammatory pathology and metabolism. (elifesciences.org)
  • This important study describes the protective role of group 2 innate lymphoid cells (ILC2s) in tissue physiology and contributes to immunity, inflammatory pathology, and metabolism in maintaining homeostasis during pregnancy. (elifesciences.org)
  • Our adaptive immunity, also called acquired immunity, is composed of lymphocyte cells that are triggered when a specific pathogen enters the body. (squareonepublishers.com)
  • Dendritic cells (DCs) link innate and adaptive immunity, initiating and regulating effector cell responses. (ashpublications.org)
  • Innate immunity resides in the skin, mucous membranes, polymorphonuclear (PMN) cells, complement system, and a select group of cells that possess cytotoxic capabilities. (medscape.com)
  • Here, the utility of OX40, a costimulatory molecule mainly expressed on activated effector T cells known to play an important role in eliminating cancer cells, was evaluated as a PET imaging biomarker to quantify and track response to immunotherapy. (aacrjournals.org)
  • Critical factors in the differentiation of effector Th cells during inflammatory bowel disease (IBD). (frontiersin.org)
  • 3-5 LILRs are encoded by a set of genes within the leukocyte receptor cluster on chromosome 19q13.4, adjacent to the killer Ig-like receptor ( KIR ) genes that are responsible for controlling NK- and CD8 T-cell survival and effector functions. (ashpublications.org)
  • Potency of Treg-suppression is dependent on strength of stimulus and on Effector:Treg cell (E:T) ratio. (bio-rad-antibodies.com)
  • B) Mean counts per minute (CPM) (+SEM) uptake of tritiated thymidine in CD25− effector T cells from healthy controls (N = 27) stimulated with mitogenic CD3/28 beads at 2:1 and 0.2:1 bead: cell ratios. (bio-rad-antibodies.com)
  • D) Mean (+SEM) percentage suppression of effector cell proliferation following activation with CD3/28 beads at 2:1 (closed boxes) and 0.2:1 (open boxes) bead: cell ratios. (bio-rad-antibodies.com)
  • They use these instructions not only for themselves - because the encounter with microbial products is not a trivial event and generates natural stress in the cells - but also for immune cells with which they cooperate. (univ-mrs.fr)
  • Uterine ILC2s are key immune cells during normal and complicated pregnancies. (elifesciences.org)
  • Both heterogeneity and tissue specialization of immune cells may hold the key to understanding the pathophysiology of some important pregnancy complications, such as miscarriage and fetal growth restriction (FGR), which is defined as the failure of the fetus to achieve its genetically determined growth potential. (elifesciences.org)
  • Despite often comprising only a small proportion of tissue resident immune cells, ILCs are present in most tissues and have been shown to orchestrate inflammatory responses to viruses, intracellular bacteria and parasites. (elifesciences.org)
  • Clinical assessment and immunological evaluation of immune cells and cytokine production was performed. (listlabs.com)
  • Here, we will review the present understanding - as garnered from studying mouse models - about the roles of various immune cells in lupus pathogenesis. (biomedcentral.com)
  • There were also significant differences in microbial composition in dust samples and in innate immune cells between the two groups of children. (medscape.com)
  • Programmed death-1 receptor (PD-L1, B7-H1) and programmed cell death protein 1 (PD-1) pathway blockade is a promising therapy for treating cancer. (nih.gov)
  • Thus, PD-L1-expressing dendritic cells and macrophages may mechanistically shape and therapeutically predict clinical efficacy of PD-L1/PD-1 blockade. (nih.gov)
  • We also show that SDF-1 is capable of inducing the migration of an SDF-1 responsive cell-line (U-937) and human PBL's from the murine circulation into the grafts in a dose dependant manner which is inhibitable by CXCR4 blockade. (biomedcentral.com)
  • CD4 + T cells are enriched in lesional tissue from patients with CD and UC and blockade or depletion of CD4 + T is effective in treating patients with IBD. (frontiersin.org)
  • Ligated DCs exhibited poor stimulatory activity for primary and memory T-cell proliferative responses, but this was substantially reversed by blockade of CD80 or its preferred ligand CTLA-4, or by depleting CD4 + CD25 + CD127 lo regulatory T cells. (ashpublications.org)
  • Additionally, expression of PD-L1 on dendritic cells and macrophages in ovarian cancer and melanoma patients correlated with the efficacy of treatment with either anti-PD-1 alone or in combination with anti-CTLA-4. (nih.gov)
  • A549-ASCoV2-NLRP1 cells have been additionally engineered to stably express the human NLRP1 inflammasome sensor. (invivogen.com)
  • Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells. (peprotech.com)
  • Additionally, organs from animal sources could be transplanted into patients currently excluded from the human organ transplantation list. (medscape.com)
  • In humans, at least two subsets of DC are known in the blood, myeloid DC (also known as interstitial or dermal DC), and plasmacytoid DC (pDC) and Langerhans cells (LC) in the tissues [ 3 ]. (biomedcentral.com)
  • 3] Porcine islet cells of Langerhans have been injected into patients with type 1 diabetes mellitus. (medscape.com)
  • Xenotransplantation involves the transplantation of nonhuman tissues or organs into human recipients. (medscape.com)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • STAT4 also signals activation of the TF T-bet, a lineage-defining factor for Th1 differentiation, which upregulates the IL-12 receptor, IFN-γ expression, and causes further expansion of Th1 cells (Figure 1 ) ( 12 ). (frontiersin.org)
  • CD80/86-CD28 interaction and others), these signals initiate a program of cell division and differentiation. (frontiersin.org)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • Thus, PS-coating can be utilized for targeted delivery of SWCNT with specified cargoes into professional phagocytes, hence for therapeutic regulation of specific populations of immune-competent cells. (cdc.gov)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
  • 13 Morphine has been shown to decrease IL-23 production in murine models, decreasing dendritic cell antimicrobial protein production. (uspharmacist.com)
  • SDS-PAGE analysis: Lane 1: MWM, Lane 2: 1.0 µg of purified Human Hsp60 protein. (enzolifesciences.com)
  • Mapping cytoskeletal protein function in cells by means of nanobodies. (scriptiebank.be)
  • A truncated C-terminal fragment of Mycobacterium tuberculosis HSP70 enhances cell-mediated immune response and longevity of the total IgG to influenza A virus M2e protein in mice. (southernbiotech.com)
  • Recombinant Human Eotaxin-2 is an 8.8 kDa protein containing 78 amino acid residues. (peprotech.com)
  • Three steps of the skin sensitizing process were depicted: protein reactivity of chemicals, activation of keratinocytes and dendritic cell like cells have been investigated. (tu-darmstadt.de)
  • The work on a new cell based protein reactivity assay showed a correlation between reduced level of detectable protein and the exposure of cells with sensitizers, although more investigation is required. (tu-darmstadt.de)
  • Moreover, the role of the L. amylovorus surface (S) -layers - symmetric, porous arrays of identical protein subunits present as the outermost layer of the cell envelope - in adherence to IPEC-1 cells was assessed using a novel approach which utilized purified cell wall fragments of the strains as carriers for the recombinantly produced S-layer proteins. (biomedcentral.com)
  • They are composed of numerous identical (glyco)protein subunits (with a 25-71 kDa size in lactobacilli), which form a regular, symmetric and porous array, completely covering the bacterial cell surface. (biomedcentral.com)
  • Since these observations, our team has collected an enormous amount of new data on the biochemical pathways involve with the acquisition by dendritic cells of unmatched immunomodulatory functions. (univ-mrs.fr)
  • In humans, it is encoded by the USP18 gene. (wikipedia.org)
  • 2007. Adenoviral gene transfer of the endogenous inhibitor IkappaBalpha into human osteoarthritis synovial fibroblasts demonstrates that several matrix metalloproteinases and aggrecanases are nuclear factor-kappaB-dependent . (cardiff.ac.uk)
  • • Cellectis SA , of Paris, announced that the Institut Gustave Roussy cancer center in Villejuif succeeded in inducing the correction of a mutation in a cell line isolated from a xeroderma pigmentosum (XP) patient by means of meganuclease-induced gene correction. (bioworld.com)
  • Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)
  • A Murine Frailty Index Based on Clinical and Laboratory Measurements: Links Between Frailty and. (deepdyve.com)
  • We aimed to develop a laboratory test-based murine FI tool (FI-Lab) and to investigate the effects of age and sex on FI-Lab scores, FI-Clinical scores, and the combination (FI-Combined), as well as to explore links between frailty and inflammation. (deepdyve.com)
  • Combination with other treatment modalities such as chemotherapy may overcome immunoresistance of cancer cells. (uni-muenchen.de)
  • The current standard of care for locally advanced non-small cell lung cancer (NSCLC) includes radiation, chemotherapy, and surgery in certain individualized cases. (frontiersin.org)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
  • In this combined immunodeficiency (SCID) cancer is low in these species (as it chapter, some aspects of this issue mice, in which the human target is in humans), which renders cancer are discussed. (who.int)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • Methods: Bone marrow-derived DC from C57BL/6 mice were loaded with UV-irradiated, syngeneic Panc02 carcinoma cells and were administered subcutaneously. (uni-muenchen.de)
  • Murine models of PCM have been used to study the several aspects of Pb infection, the outcomes varying from cure to a progressive granulomatous disease, according to the susceptibility of the strain of mice [ 15 ]. (hindawi.com)
  • Providing an underlying mechanism for this connection, however, is recent work that showed DMPA weakens genital mucosal barrier function in mice and humans and respectively promotes susceptibility of wild-type and humanized mice to genital infection with HSV type 2 and HIV type 1. (stanford.edu)
  • In this study, we show that compared with mice in estrus, treatment of mice with DMPA or NET-EN significantly decreased genital levels of the cell-cell adhesion molecule desmoglein-1 and increased genital mucosal permeability. (stanford.edu)
  • These effects, however, were more pronounced in DMPA- versus NET-EN-treated mice. (stanford.edu)
  • Likewise, we detected comparable mortality rates in DMPA- and NET-EN-treated wild-type and humanized mice after intravaginal infection with HSV type 2 or cell-associated HIV type 1, respectively, but NET-EN treatment was associated with slower onset of HSV-induced genital pathology and lower burden of systemic HIV disease. (stanford.edu)
  • In mice, LT b etaR signaling is important for development and function of HEV(2), Dendritic cells(3), and Mast cells(4). (ancell.com)
  • Importantly, PSA binding by B cells is essential for induction of regulatory CD4 + and CD8 + T cells secreting IL-10 to control innate inflammatory responses, consistent with the lack of PSA mediated protection in Rag −/− , B cell- and IL-10-deficient mice. (nature.com)
  • B cell-targeted therapies are effective for treating multiple different kidney diseases in humans and also protect mice from Adriamycin nephropathy. (bvsalud.org)
  • Because glomerular IgM is frequently seen in both humans and mice with "nonimmune" forms of glomerular disease, we hypothesized that natural IgM binds to epitopes displayed in the injured glomerulus, exacerbating injury. (bvsalud.org)
  • An FI based on abnormalities in standard lab test results, analogous to the FI-Lab used in humans (6), has not yet been developed in mice. (deepdyve.com)
  • Animal models of human BTK mutations are confined to mice at this time. (medscape.com)
  • It was reported that human USP18 is a novel factor potentially contributing to HIV replication by blocking the antiviral function of p21 in differentiated human myeloid cells. (wikipedia.org)
  • N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer. (thermofisher.com)
  • Previous studies have shown great potential of using the CD11c molecule as a target on the dendritic cells. (aau.dk)
  • However, studies are focusing on the murine model and not much is known about the potential of targeting CD11c in human models. (aau.dk)
  • 5. Arnaboldi PM, Roth-Walter F, Mayer L. Suppression of Th1 and Th17, but not Th2, responses in a CD8 + T cell mediated model of oral tolerance. (southernbiotech.com)
  • CD4 + T cells are key in mediating the host protective and homeostatic responses. (frontiersin.org)
  • Dendritic cells (DCs) play pivotal roles in the initiation, regulation, and maintenance of immune responses. (ashpublications.org)
  • Hiding in the entryways used by pathogens, dendritic cells locate infectious agents, ingest them and release biochemical signals to alert the first line of defensive cells in the body and draw them to the site of the infection. (univ-mrs.fr)
  • Th1 cells are important for protecting against infectious pathogens. (frontiersin.org)
  • These cells learn to identify the invading pathogens and hunt them down. (squareonepublishers.com)
  • Exposure of murine and human DCs to dolastatins significantly increased their capacity to prime T cells. (biomedcentral.com)
  • These features remained stable even after exposure of the cells to bacterial LPS. (ashpublications.org)
  • We have shown that huPLN transplants are viable and are vascularised by the murine circulation, forming functional anastomoses with transplant vessels. (biomedcentral.com)
  • LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification. (lu.se)
  • In the absence of functional Btk, mature B cells that express surface Ig and the marker CD19 are few to absent. (medscape.com)
  • SDF-1 (CXCL12), a CXC chemokine, has a primary role in signalling the recruitment of haematopoietic stem-cell precursors to the bone marrow during embryonic development. (biomedcentral.com)
  • Here, we used a murine pancreatic carcinoma model to investigate whether combination with gemcitabine increases therapeutic efficacy of DCbased vaccination. (uni-muenchen.de)
  • At that time, data were insufficient to assess the efficacy of ZDV as a prophylactic agent in humans or the toxicity of this drug in persons not infected with HIV. (cdc.gov)
  • GFP with the NF-κB inducer TNF-α and stimulation using SARS-CoV-2 infectious particles, A549-ASCoV2-NLRP1 cells display ASC specks, visible as fluorescent green dots and undergo pyroptosis, readily measurable by the LDH assay. (invivogen.com)
  • Pemphigus vulgaris (PV) is a life-threate- disorders and to shed a light on the role of ning blistering skin disease in which pa- mast cells in autoimmune diseases [ 7 ]. (who.int)