• Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells. (uni-muenchen.de)
  • In this study, we analysed the intracellular carbon metabolism of enteroinvasive Escherichia coli (EIEC HN280 and EIEC 4608-58) and Salmonella enterica Serovar Typhimurium (Stm 14028) replicating in epithelial colorectal adenocarcinoma cells (Caco-2). (uni-muenchen.de)
  • GENERAL INFORMATION Human Colorectal Adenocarcinoma Cells (SW480) was derived from a Colorectal Adenocarcinoma of a 50-year old Caucasian male. (ubpone.com)
  • RFP-Human Colorectal Adenocarcinoma Cells (SW480) are selected from the Colorectal Adenocarcinoma Cells (SW480) (UBP-0074) transfected with RFP expressing lentiviruses resistant to puromycin. (ubpone.com)
  • Human Colorectal Adenocarcinoma Cells (SW480) are tested negative for HIV-1, HBV, HCV, and mycoplasma. (ubpone.com)
  • Part of: AstraZeneca Colorectal cell line (AZCL) panel. (cellosaurus.org)
  • Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. (cellosaurus.org)
  • Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines. (cellosaurus.org)
  • Cell growth, global phosphotyrosine elevation, and c-Met phosphorylation through Src family kinases in colorectal cancer cells. (cellosaurus.org)
  • 5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency. (cellosaurus.org)
  • Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer. (cellosaurus.org)
  • In this study, we have tested whether telomerase might be a target for the antitumor effect of fatty acids using DLD-1 colorectal adenocarcinoma cells. (elsevierpure.com)
  • Eitsuka, T , Nakagawa, K & Miyazawa, T 2004, ' Dual mechanisms for telomerase inhibition in DLD-1 human colorectal adenocarcinoma cells by polyunsaturated fatty acids ', BioFactors , vol. 21, no. 1-4, pp. 19-21. (elsevierpure.com)
  • In this study, we examined CEPA-induced gene expression in DLD-1 colorectal adenocarcinoma human cells carrying a mutant p53, in order to understand the details of CEPA-induced apoptosis via lipid peroxidation. (elsevierpure.com)
  • The diversity of histologic composition reflects the inter- and intra-tumor heterogeneity of lung adenocarcinomas (LUADs) macroscopically. (nih.gov)
  • NR0B1 protein was detected in cancerous tissues of more than 50% of human lung adenocarcinoma (ADCA) cases and tended to be expressed in low-differentiated cancerous tissues obtained from males. (oncotarget.com)
  • We detected miR-27a expression in two lung adenocarcinoma cell lines, A549 and A549/CDDP, and then investigated the effects of miR-27a on the metastasis and the chemosensitivity of cancer cells, using both gain- and loss-of-function studies. (biomedcentral.com)
  • The correlation between miR-27a level and chemoresistance was further investigated in clinical lung adenocarcinoma specimens. (biomedcentral.com)
  • miR-27a was significantly up-regulated in cisplatin-resistant lung adenocarcinoma A549/CDDP cells compared with parental A549 cells. (biomedcentral.com)
  • miR-27a regulates epithelial-mesenchymal transition (EMT) and cisplatin resistance in vitro and modulates response of lung adenocarcinoma cells to cisplatin in vivo . (biomedcentral.com)
  • Small interfering RNA-mediated RKIP knockdown revealed similar effects as that of ectopic miR-27a expression, while overexpression of RKIP attenuated the function of miR-27a in lung adenocarcinoma cells. (biomedcentral.com)
  • Increased miR-27a expression was also detected in tumor tissues sampled from lung adenocarcinoma patients treated with cisplatin-based chemotherapy and was proved to be correlated with low expression of RKIP, decreased sensitivity to cisplatin, and poor prognosis. (biomedcentral.com)
  • Our results suggest that up-regulation of miR-27a could suppress RKIP expression and in turn contribute to chemoresistance of lung adenocarcinoma cells to cisplatin. (biomedcentral.com)
  • In this study, we observed that miR-27a is significantly upregulated in cisplatin-resistant human lung adenocarcinoma A549/CDDP cells compared with parental A549 cells. (biomedcentral.com)
  • Next we explored the roles of miR-27a and its target Raf Kinase Inhibitory Protein (RKIP) in regulating cisplatin resistance and metastasis in lung adenocarcinoma. (biomedcentral.com)
  • Finally, we correlated the expression of miR-27a and RKIP with the chemotherapeutic status and prognosis of lung adenocarcinoma patients. (biomedcentral.com)
  • Our results show that miR-27a has the potential as key regulatory factors for the chemotherapy resistance and metastasis of lung adenocarcinoma. (biomedcentral.com)
  • To better understand the biological mechanisms of chemoresistance in lung adenocarcinoma cells and search for the reversion opportunities, we made use of a cisplatin-sensitive and derived resistant lung cancer cell line pair (A549 and A549/ CDDP). (biomedcentral.com)
  • Here we provide an extremely simple, rapid, direct, sensitive and specific method to quantify for the first time intracellular GSH in human prostate cancer cells (DU145) and human lung adenocarcinoma cells (A549) using an electrochemical detector coupled to a high performance liquid chromatograph (HPLC-ECD). (unict.it)
  • Delivery of siRNA-peptide nanoplexes co-targeting MK2 and XPA to pre-existing p53-deficient tumors in a highly aggressive, immunocompetent mouse model of lung adenocarcinoma improves long-term survival and cisplatin response beyond those of the synthetic lethal p53 mutant/MK2 combination alone. (nature.com)
  • Lung cancer is divided into two major groups: non small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). (scielo.br)
  • 2004) Akt phosphorylation and gefitinib efficacy in patients with advanced non-small-cell lung cancer. (scielo.br)
  • 2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (scielo.br)
  • 2005) Activating mutations in the tyrosine kinase domain of the epidermal growth factor receptor are associated with improved survival in gefitinib-treated chemorefractory lung adenocarcinomas. (scielo.br)
  • Metz H, Busch SE, Hanke ML, Kargl J, Kim KH and Houghton M (2014) Insulin receptor substrate-1 regulates immune cell content in lung adenocarcinoma. (scielo.br)
  • Here, we showed that hepatocyte growth factor (HGF), a ligand of MET oncoprotein, induces gefitinib resistance of lung adenocarcinoma cells with EGFR -activating mutations by restoring the phosphatidylinositol 3-kinase/Akt signaling pathway via phosphorylation of MET, but not EGFR or ErbB3. (aacrjournals.org)
  • Here, we investigate the effects of the photovoltaic activity of p-i-n nanowire arrays on A549 human lung adenocarcinoma cells. (lu.se)
  • Comprehensive investigation of oncogenic driver mutations in Chinese non-small cell lung cancer patients. (cdc.gov)
  • Detection of Novel NRG1, EGFR, and MET Fusions in Lung Adenocarcinomas in the Chinese Population. (cdc.gov)
  • Comprehensive analysis of the clinicopathological features, targetable profile, and prognosis of mucinous adenocarcinoma of the lung. (cdc.gov)
  • A case of multiple primary lung adenocarcinoma with a CD74-NRG1 fusion protein and HER2 mutation benefit from combined target therapy. (cdc.gov)
  • Direct extension can also occur through a vascular route, such as the spread of renal cell cancer or testicular germ cell cancer as a tumor thrombus to the lung via the inferior vena cava and the right side of the heart. (medscape.com)
  • Colchicine, nocodazole and taxol were found to induce differentiation and apoptosis in HT29 cells. (tau.ac.il)
  • Treatment with colchicine or forskolin induced the association of E-cadherin to the cytoskeleton fraction of subconfluent HT29 cells. (tau.ac.il)
  • The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
  • To investigate the mechanism of EESR-mediated cytotoxicity, HT29 cells were treated with various concentrations of EESR and the induction of cell cycle arrest and apoptosis was analyzed by flow cytometry, 4,6-diamidino-2-phenylindole staining, and Western blot analysis. (jcpjournal.org)
  • EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. (jcpjournal.org)
  • Volatile aldehydes are enriched in esophageal adenocarcinoma (EAC) patients' breath and could improve early diagnosis, however the mechanisms of their production are unknown. (nature.com)
  • According to the above explanations, we tried to prepare and formulate a chemotherapeutic drug (gold nanoparticles in aqueous medium using Rhus coriaria L. fruit aqueous extract) for the treatment of several types of esophageal cancer including human esophageal squamous cell carcinoma, human Caucasian esophageal carcinoma, adenocarcinoma of the gastroesophageal junction, and distal esophageal adenocarcinoma. (ac.ir)
  • To survey the cytotoxicity and anti-esophageal cancer effects of HAuCl4, R. coriaria aqueous extract, and gold nanoparticles, dye compound 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) assay was used on the human esophageal squamous cell carcinoma (KYSE-270), human Caucasian esophageal carcinoma (OE33), adenocarcinoma of the gastroesophageal junction (ESO26), and distal esophageal adenocarcinoma (FLO-1) cell lines. (ac.ir)
  • After approving the above results in the clinical trial studies, these gold nanoparticles can be used as a chemotherapeutic drug for the treatment of several types of esophageal cancer such as human esophageal squamous cell carcinoma, human Caucasian esophageal carcinoma, adenocarcinoma of the gastroesophageal junction, and distal esophageal adenocarcinoma. (ac.ir)
  • Human esophageal adenocarcinoma (EAC) cell lines have made a substantial contribution to elucidating mechanisms of carcinogenesis and drug discovery. (eur.nl)
  • Antitumor Effect of Galectin-9 on Cell Proliferation and Tumor Growth of Human Duodenal Adenocarcinoma Cells. (bvsalud.org)
  • Cell proliferation was examined in HuTu-80 cells using a Cell Counting Kit-8 assay. (bvsalud.org)
  • Gal-9 suppressed the proliferation of HuTu-80 via blockade of the G0 to G1 cell cycle transition. (bvsalud.org)
  • Ductal cells have a proliferation rate of about 0.5% in normal adults, but mitotic activity goes up when the pancreas is damaged. (wikipedia.org)
  • Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. (jcpjournal.org)
  • 6 , 7 Therefore, the inhibition of cancer cell proliferation by inducing cell cycle arrest and apoptosis is being an important preventive strategy against cancer. (jcpjournal.org)
  • SOX9 promotes nasopharyngeal carcinoma cell proliferation, migration and invasion through BMP2 and mTOR signaling. (cancerindex.org)
  • Overexpression of SOX9 promoted NPC cell proliferation, migration and invasion. (cancerindex.org)
  • Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. (scielo.br)
  • Results indicated that 1) low dose MW-COOH and MW-NH2 exposure caused significant increases in cell proliferation compared to controls starting at 1 M and persisted over 6 M exposure. (cdc.gov)
  • We showed that overexpression of DKK3 can decrease cell invasion, proliferation, and colony forming ability of GBC cells. (amrita.edu)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • However, there is no difference in the proportion of dormant cells when the p-i-n nanowires are coated with oxide, which suggests that carrier injection in the cell medium (in this case, the release of electrons from the tip of the nanowires) is an important factor for modulating cell proliferation on photovoltaic nanowires. (lu.se)
  • 1980), lates cell proliferation in the rat respiratory tract (Roemer et al. (cdc.gov)
  • This study was designed to investigate the survival difference between pure adenocarcinoma (AC) and squamous cell carcinoma (SCC) in early cervical cancer (FIGO stage IB-IIA) after radical hysterectomy with or without adjuvant therapy performed at a single institution. (nih.gov)
  • This cancer is known as squamous cell carcinoma. (medlineplus.gov)
  • The cause of squamous cell carcinoma of the vagina is unknown. (medlineplus.gov)
  • But a history of cervical cancer is common in women with squamous cell carcinoma of the vagina. (medlineplus.gov)
  • Most women with squamous cell cancer of the vagina are over 50. (medlineplus.gov)
  • However, a significant shortcoming of Pap cytology is that it was developed to detect squamous cell carcinoma, not adenocarcinoma. (medscape.com)
  • SC-1 cells grow in vitro floating free state and tend to from clusters which easily disaggregated into single cell suspension with agitation with a pipette. (e-crt.org)
  • The advantages of using cannabinoids have been evaluated in vitro and in vivo models of several human cancers. (cannabisclinicians.org)
  • Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro. (ac.rs)
  • Unfortunately, at the time of clinical diagnosis, human prostate cancers mostly present themselves as heterogeneous entities - hormone-dependent and hormone-independent, and proliferating and non-proliferating. (biomedcentral.com)
  • Most primary vaginal cancers start in skin-like cells called squamous cells. (medlineplus.gov)
  • Over the past decade, the identification of stem-like cells as drivers of tumor initiation, recurrence, and metastasis spread in a variety of human cancers had a tremendous impact in basic and translational cancer research. (hindawi.com)
  • As mentioned, the gold nanoparticles had significant anti-esophageal cancer effects against the above cancers especially human Caucasian esophageal carcinoma. (ac.ir)
  • The concept of synthetic lethality (SL) holds great promise for the treatment of human cancers, best exemplified by the now widespread use of PARP inhibitors in BRCA mutant cancers. (nature.com)
  • Loss of DKK3 gene is associated with a possible tumor suppressor role in human cancers. (amrita.edu)
  • Cancer registries do not routinely collect information on HPV DNA presence in cancer tissues, and HPV-associated cancers defined by anatomic site and cell type include cancers not caused by HPV. (cdc.gov)
  • It represents 1.64% of intervention could reduce the risk for amination using a long forward viewing all cancers in Egypt, according to the adenocarcinoma of the distal stomach instrument (Fuji, EG 250D video). (who.int)
  • Aims: The aim of this study was to sensitize the resistant breast adenocarcinoma cells towards Tumour Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL)-induced apoptosis. (eurekaselect.com)
  • Objective: Therefore, this research investigated whether the combination of Trichostatin A (TSA) and Zebularine (Zeb) (TZ) followed by TRAIL (TZT) could sensitize the human breast adenocarcinoma cells towards apoptosis. (eurekaselect.com)
  • Methods: The breast adenocarcinoma cells, MDA-MB-231, MCF-7 and E-MDA-MB-231 (E-cadherin re-expressed MDA-MB-231) were treated with TSA, Zeb, TZ, TRAIL and TZT. (eurekaselect.com)
  • Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis, mainly because the tumors are detected too late for effective treatment or for developing suitable therapeutics. (elsevierpure.com)
  • Gastric cancer (GC) is a common malignant tumor originating from gastric mucosa epithelial cells, and its onset is relatively insidious. (frontiersin.org)
  • The cells were subjected to hematoxylin and eosin (H & E) staining and FITC-Annexin V/Propidium Iodide apoptosis detection prior to proteome profiling. (eurekaselect.com)
  • Results: Based on morphological observation, apoptosis was induced in all cells treated with all treatment regimens though it was more evident for the TZT-treated cells. (eurekaselect.com)
  • For E-MDA-MB- 231, down-regulation of catalase, paraoxonase-2 (PON2), clusterin, an inhibitor of apoptosis proteins (IAPs) and cell stress proteins validated the notion that E-cadherin re-expression enhances TZT anti-cancer efficacy. (eurekaselect.com)
  • The objective of the current studies was to examine the role of caspase 3 (CPP32) and NAD(P)H:quinone oxidoreductase (NQO1) in the signaling of genistein-and β-lapachone (bLap)-induced apoptosis in human prostate carcinoma cells PC3. (biomedcentral.com)
  • implying that NQO1 pathway may not be the main target for genistein-induced apoptosis in PC3 cells. (biomedcentral.com)
  • Apoptosis was characterized by detachment of round cells from the cell layer, condensation of nuclear DNA and annexin V binding to cell surfaces. (tau.ac.il)
  • Galectin -9 (Gal-9) induces tumor cell apoptosis in lymphoma and other malignant cell types. (bvsalud.org)
  • Cell cycle analysis , apoptosis array, and microRNA expression analysis were performed to identify the effect of Gal-9 on HuTu-80 cells . (bvsalud.org)
  • Additionally, Gal-9 induced apoptosis , and the expression of cleaved caspase-3 was increased in Gal-9-treated HuTu-80 cells according to the apoptosis array. (bvsalud.org)
  • Regarding PDAC, activation of cannabinoid receptors was found to induce pancreatic cancer cell apoptosis without affecting the normal pancreas cells. (cannabisclinicians.org)
  • CEPA treatment caused up-regulation of expression of genes induced by p53 and activation of the mitochondrial apoptosis pathway via Bax and the death pathway via TRAIL, leading to apoptosis of DLD-1 cells. (elsevierpure.com)
  • This is the first such gene expression analysis of the effects of CEPA, and our results confirm that CEPA induces lipid peroxidation, activates mutant p53, and causes p53-dependent apoptosis in DLD-1 cells. (elsevierpure.com)
  • Cancer cells can grow unusually via limitless number of cell division and can avoid apoptosis. (jcpjournal.org)
  • Frequent NRG1 fusions in Caucasian pulmonary mucinous adenocarcinoma predicted by Phospho-ErbB3 expression. (cdc.gov)
  • Because most tumors are deficient in one or more aspects of the function of the p53 tumor suppressor, either as a consequence of mutations within p53, or impairment of upstream and downstream modulators of p53 activity 19 , targeting MK2 has the potential to selectively enhance tumor cell killing without increasing the genotoxic effects of chemotherapy on normal p53-wild type tissues. (nature.com)
  • We observed low expression of DKK3 in gallbladder adenocarcinoma tumors and highly invasive GBC cell lines. (amrita.edu)
  • The high genomic similarities (but certainly not completely identical) between the EAC cell lines and their original tumors provide rational for their use. (eur.nl)
  • Accordingly, the expression of human HMGCS2 is down-regulated in 90% of Myc-dependent colon and rectum tumors. (aacrjournals.org)
  • Home 1 / Cancer 2 / Cannabidiol and Oxygen-Ozone Combination Induce Cytotoxicity in Human Pancreatic. (cannabisclinicians.org)
  • Herein, we evaluate the anticancer effect of CBD and O2/O3, alone or in combination, on two human PDAC cell lines, PANC-1 and MiaPaCa-2, examining expression profiles of 92 pancreatic adenocarcinoma associated genes, cytotoxicity, migration properties, and cell death. (cannabisclinicians.org)
  • AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. (ac.rs)
  • The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. (ac.rs)
  • Maximum cytotoxicity of Spt on MCF-7 cells was observed at Spt 5 µg/mL. (manipal.edu)
  • However, the heterogeneous initial populations, different cellular states, and active memory of pancreatic epithelial cells challenge for making iPSC-like lines from human PDAC. (elsevierpure.com)
  • Spt did not show any cytotoxic effects on MCF-10 A normal human breast epithelial cells, indicating Spt has no effect on normal cells. (manipal.edu)
  • In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
  • SC-1 cells contain mucinous substance in cytoplasm which is demonstrable upon PAS and mucicarmine staining. (e-crt.org)
  • Comprehensive Molecular and Clinicopathologic Analysis of 200 Pulmonary Invasive Mucinous Adenocarcinomas Identifies Distinct Characteristics of Molecular Subtypes. (cdc.gov)
  • Also, genotoxicity of AuNPL in HaCaT cells was investigated by the single cell alkaline comet assay. (ac.rs)
  • At concentrations required for PPARγ activation and ANGPTL4 induction in colon adenocarcinoma cells, SCFA do not stimulate PPARγ in mouse 3T3-L1 and human SGBS adipocytes, suggesting that SCFA act as selective PPARγ modulators (SPPARM), which is supported by coactivator peptide recruitment assay and structural modeling. (uea.ac.uk)
  • Cell viability for Spt treated cells was studied using MTT assay. (manipal.edu)
  • RAMOS Cell Line human has been used to carry out a MTT (colorimetric assay) cell viability assay to measure calcium ionophore-induced cell death in Ramos cells under different conditions and to determine the expression of ZFP36 ring finger protein like 1 (ZFP36L1) in Ramos B cells during different stages of B cell differentiation. (sigmaaldrich.com)
  • n HeLa cells were demonstrated by flow cytometry. (ac.rs)
  • AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. (ac.rs)
  • Human wild-type HeLa cell line ( ab255928 ). (abcam.com)
  • We have cultured A549 cells on top of vertical arrays of indium phosphide p-i-n nanowires (photovoltaic nanowires), with and without illumination to assess the effects of the nanowire photovoltaic activity. (lu.se)
  • One human colon adenocarcinoma cell line, SC-1 (Seoul National University, Adenocarcinoma of the Colon), has been established from a metastatic tumor mesocolon of distal descending colon of a 71 years old Korean male patient. (e-crt.org)
  • These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in combination with repair of cisplatin-DNA lesions in vivo using RNAi nanocarriers, and motivate further exploration of ASL as a generalized strategy to improve cancer treatment. (nature.com)
  • We showed that when an iPS-like cell line, designated 10-22, derived from human recurrent PDAC, was injected into immunodeficient mice, the cells consistently recapitulated preinvasive, pancreatic intraepithelial neoplasia (PanIN) to invasive stages of human PDAC. (elsevierpure.com)
  • The procedure to derive iPSCs from human PDAC is principally the same as the procedure to generate iPSCs from normal human fibroblast. (elsevierpure.com)
  • Herein, we describe how to create and maintain iPSC-like line from human PDAC by lentiviral transduction of reprogramming factors. (elsevierpure.com)
  • The plasticity and heterogeneity of fibroblasts in the tumor microenvironment of pancreatic ductal adenocarcinoma (PDAC) has emerged as a key factor in determining tumor growth and therapeutic response. (elifesciences.org)
  • Exposure results in pulmonary fibrosis, biopersistence, extrapulmonary transport and promotion of adenocarcinoma and sarcomatous mesothelioma. (cdc.gov)
  • Copper ions bind to biomolecules (e.g., peptides and proteins) playing an essential role in many biological and physiological pathways in the human body. (mdpi.com)
  • Differentiation was characterized by flattening of the cells, formation of brush borders on apical surfaces and tight junctions between adjacent cells. (tau.ac.il)
  • Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. (invivogen.com)
  • Analysis of the genome sequences of the major human bacterial pathogens has provided a large amount of information concerning their metabolic potential. (uni-muenchen.de)
  • It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)
  • Overall, our data suggest that ALKBH6 is required to maintain the integrity of the genome and promote cell survival of pancreatic cancer cells. (frontiersin.org)
  • Treatment of GBC cell lines with demethylating agent 5-Aza- 2'-deoxycytidine enhances its expression, establishing impact of methylation on DKK3 expression. (amrita.edu)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • All seeding densities should be based on cell counts gained by established methods. (abcam.com)
  • Insights into the oncological characteristics and tumor microenvironment (TME) of different histologic subtypes of LUAD at the single-cell level can help identify potential therapeutic vulnerabilities and combinational approaches to improve the survival of LUAD patients. (nih.gov)
  • Remarkably, tumor cells from solid LUADs upregulate energy and substance metabolic activities, particularly the folate-mediated one-carbon metabolism and the key gene MTHFD2, which could serve as a potential therapeutic target. (nih.gov)
  • Duodenal adenocarcinoma is a rare malignancy , and there are insufficient data to determine a standard therapeutic approach. (bvsalud.org)
  • These data demonstrate the therapeutic potential of Gal-9 and provide molecular mechanistic insights into its antitumor effect in HuTu-80 cells . (bvsalud.org)
  • Septilin (Spt), a polyherbal drug and its therapeutic potential is huge but there is a scarcity of studies on its cytotoxic potential on cancer cells. (manipal.edu)
  • Because BRCA mutations are observed in fewer than 10% of cancer patients (cBioPortal: 6.7%) 11 , 12 , 13 the identification of additional genes that share synthetic lethal sensitivity relationships with mutated oncogenes or tumor suppressors would greatly enhance the implementation of tumor cell-specific synthetic lethal sensitivity to improve an anticancer therapeutic response. (nature.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • In the present study, up-regulation of SOX9 was observed in both NPC tissues and different NPC cells. (cancerindex.org)
  • Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells. (cellosaurus.org)
  • Therefore, chemotherapy-induced EMT in tumor cells has been linked to chemotherapeutic resistance and metastasis. (biomedcentral.com)
  • Understanding how cancer stem cells affect tumor heterogeneity, the contribution of resident stem and progenitor cells to the tumor niche, and the search for true cancer stem cell markers are other examples of scientific challenges in this fast-growing research area. (hindawi.com)
  • and renal cell carcinoma. (nih.gov)
  • Tiwari, B.S. Programmed cell death: A process of death for survival-How far terminology pertinent for cell death in unicellular organisms. (eurekaselect.com)
  • In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
  • The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)
  • As an emerging cancer immunotherapy, oncolytic virotherapies (OVTs) can not only selectively lyse cancer cells, but also induce a systemic antitumor immune response. (frontiersin.org)
  • Thus, even a small insulin-like effect of IGF-I could be more important than that of insulin itself, were it not for the IGFBPs that control the availability and activity of IGF-I. In fact, intravenous infusion of recombinant human IGF-I (rhIGF-I) can induce hypoglycemia, especially in the IGFBP3 deficient state. (medscape.com)
  • Several alkylating agents are commonly used in chemotherapy but may induce DNA damage to cancer cells as well as normal cells. (frontiersin.org)
  • Ductal cells comprise about 10% of the pancreas by number and about 4% in volume. (wikipedia.org)
  • While ductal cells are a minor type of cell in the adult pancreas, they have a critical function besides making the network that transfers enzymes from acini to the digestive tract. (wikipedia.org)
  • The primary function of pancreas ductal cells is to secrete a bicarbonate-rich, isotonic fluid. (wikipedia.org)
  • Furthermore, unlike other exocrine glands, the pancreas does not have myoepithelial cells around the ducts. (wikipedia.org)
  • Meanwhile, mature ducts have a restricted ability to transdifferentiate to other types of cells, even when the pancreas is injured. (wikipedia.org)
  • There is disagreement about the plasticity potential of ductal cells in the adult pancreas. (wikipedia.org)
  • In the embryonic pancreas, the endocrine and exocrine cells originate in the pancreatic ducts as progenitor cells. (wikipedia.org)
  • This proposes the idea that there might be a subgroup of ductal cells that have the ability to dedifferentiate and generate endocrine cells when there is an injury to the pancreas. (wikipedia.org)
  • Essentially, ductal cells function in retaining the adult pancreas β cell mass when injured. (wikipedia.org)
  • However, there is a possibility that this capability is limited to a subtype of ductal cells only, meaning this cannot be a main pathway for pancreas regeneration. (wikipedia.org)
  • Multicentre case-control studies clearly demonstrated a cervical cancer risk associated with infection with a variety of human papillomaviruses (HPV) other than types 16 and 18. (who.int)
  • The shift from Papanicolaou (Pap) cytology to direct testing for the human papillomavirus (HPV) represents a major advance in cervical cancer screening. (medscape.com)
  • TRAIL is a potential anticancer candidate for targeted treatment due to its selective killing effects on neoplastic cells. (eurekaselect.com)
  • GSH determination in cancer cells are importance because GSH has been involved in the development of resistance to several anticancer drugs. (unict.it)
  • Our findings suggest that Spt can be used in combination with an anticancer drug Csp to increase its efficacy and/or to minimize its side effects on normal cells. (manipal.edu)
  • Here, we investigated the antitumor effect of Gal-9 in HuTu-80 duodenal adenocarcinoma cells . (bvsalud.org)
  • The hormone secretin stimulates ductal cells and is responsible for maintaining the duodenal pH and preventing duodenal injury from acidic chyme. (wikipedia.org)
  • DNA microarray analysis of 9970 genes was performed by comparison of CEPA-treated DLD-1 cells with untreated DLD-1 cells, thereby allowing determination of the differential gene expression profile induced by CEPA in these cells. (elsevierpure.com)
  • SL originally described a relationship between two genes, where alteration of either gene alone results in viable cells, but alteration (mutation, loss, or inhibition) of both genes simultaneously was lethal. (nature.com)
  • Model research on EAC relies almost entirely on a relatively small set of established tumor cell lines because appropriate animal models are lacking. (eur.nl)
  • Serious adverse effects and low selectivity to cancer cells are the main obstacles of long term therapy with Tamoxifen (Tmx). (springer.com)
  • Moreover, the cytotoxic activity of the optimized formula on both breast cancer cells (MCF-7) and normal cells (BHK) showed enhanced selectivity (9.4 folds) on cancerous cells with IC 50 values 4.7 ± 1.5 and 44.3 ± 1.3 μg/ml on cancer and normal cells, respectively. (springer.com)
  • While, free Tmx exhibited lower selectivity (2.5 folds) than optimized nano-vesicles on cancer cells with IC 50 values of 9.0 ± 1.1 μg/ml and 22.5 ± 5.3 μg/ml on MCF-7 and BHK cells, respectively. (springer.com)
  • Therefore chemotherapeutic strategies should focus on eradicating all cancer cells irrespective of state of growth or sensitivity to hormone. (biomedcentral.com)
  • The facts that prostate cancer cells retain the inherent apoptotic machinery potentially subject them to an appropriate efficacious chemotherapeutic intervention. (biomedcentral.com)
  • Reprogramming cancer cells to pluripotency by induced pluripotent stem cell (iPSC) technology, which can be then programmed back to their original cellular state, allows for studying the dynamic events in the course of the disease progression. (elsevierpure.com)
  • Advances in this field point to a more complex model of tumor development than previously envisioned, in which the stem-like phenotype may be dynamically acquired by cancer cells. (hindawi.com)
  • Unfortunately, patients are already in the terminal stage at this time, and the cancer cells have already invaded the surrounding organs or metastasized far away, which leads to the loss of the curative chance for most patients ( 1 ). (frontiersin.org)
  • Cancer cells that are defective in p53 function are deficient in their ability to transcriptionally upregulate the CDK inhibitor p21 after genotoxic stress. (nature.com)
  • Ribosomal protein L10 in mitochondria serves as a regulator for ROS level in pancreatic cancer cells. (nih.gov)
  • Here, we tested whether ALKBH6 plays a significant role in preventing alkylating DNA damage and decreasing genomic instability in pancreatic cancer cells. (frontiersin.org)
  • Pancreatic ductal adenocarcinoma has a poor clinical outcome and responses to immunotherapy are suboptimal. (elifesciences.org)
  • Here the authors use innovative approaches to combine spatial profiling with single cell transcriptomics to define tumor-proximal populations of fibroblasts that predict clinical outcome. (elifesciences.org)
  • Discrepancies in stromal cell function, evidenced by varying degrees of stromal remodeling and fibrosis, may also contribute to the specific immune phenotype of solid LUADs. (nih.gov)
  • Universal Full Growth Medium (UBP-01B) is recommended to culture the cells. (ubpone.com)
  • The tumor re-growth that occurs after post-treatment remission is largely due to progression of initially androgen-dependent to androgen-independent cancer cell [ 4 ] and/or non-proliferating to proliferating tumor cells. (biomedcentral.com)
  • The human colon adenocarcinoma cell line HT29 displays an undifferentiated phenotype under standard growth conditions. (tau.ac.il)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • Not only do these wrap pancreatic tumours in a dense, protective layer, they also foster complex relationships with the cancerous cells: some fibroblasts may fuel tumour growth, while other may help to contain its spread. (elifesciences.org)
  • EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
  • The abnormal cell growth is one of the critical characteristics of cancer for invasion or spreading out to other parts of the body. (jcpjournal.org)
  • It responds poorly to immunotherapy for instance, despite this approach often succeeding in enlisting immune cells to fight tumours in other organs. (elifesciences.org)
  • Cell Death Dis, 2023 Jun 7. (nih.gov)
  • Ductal cells refer to the epithelial cell lining of the pancreatic duct that deliver enzymes from the acinar cells to the duodenum. (wikipedia.org)
  • Ductal cells mix their production with acinar cells to make up the pancreatic juice. (wikipedia.org)
  • Its function is to secrete bicarbonate and mucins and to form the tubule network that transfers enzymes made by acinar cells to the duodenum. (wikipedia.org)
  • We first asked whether a cell-autonomous mechanism could contribute to exhaled biomarkers, so we began by searching for thematic differences in metabolic gene expression between EAC and relevant control tissue in archived transcriptomic datasets. (nature.com)
  • MiRNA microarrays revealed that Gal-9 altered the expression of miRNAs in HuTu-80 cells . (bvsalud.org)
  • NR0B1 expression was not detected in 293T cells, although it contains a hypomethylated NR0B1 promoter. (oncotarget.com)
  • Treating 293T cells with a histone deacetylase inhibitor increased acetylated histone H4 binding to the NR0B1 promoter and activated NR0B1 expression. (oncotarget.com)
  • EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. (jcpjournal.org)
  • We found that DKK3 expression levels were low in GBC patients and cell lines. (amrita.edu)
  • HT29-Lucia™ AhR cells are engineered from the human HT-29 colon adenocarcinoma cell line for the study of the aryl hydrocarbon receptor (AhR) genomic signaling induction (see details), by monitoring the activity of the Lucia luciferase reporter protein. (invivogen.com)
  • HT29 Cell Line. (invivogen.com)
  • The stability of this cell line for 20 passages following thawing has been verified. (invivogen.com)
  • Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)
  • Re-suspend the cell pellet with 10 or 15ml Full medium and transfer 5 ml each into 2 or 3 pre-coated T25 flasks (for 1/2 to 1/3 subculture ratio). (ubpone.com)
  • Now on the 214th day, October 22, 1984, 41th subculture with floating cells has been done. (e-crt.org)
  • Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry. (cdc.gov)