Ht29 human colon cancer cells ht29 cells. Medical search. Frequent questions

Croton lechleri sap and isolated alkaloid taspine exhibit inhibition against human melanoma SK23 and colon cancer HT29 cell lines. (rain-tree.com)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
Activation of TRPV1 by capsaicin caused significant inhibition of cancer cell growth and induced apoptosis and necrosis. (dovepress.com)
The extract also showed a dose dependent growth inhibition of all tested cell lines, IC 50 values against HCT-116, HUVEC, CCD-18CO and HT-29 were 14, 23, 50 and 52 μg mL -1 , respectively. (scialert.net)
Activation of AMPK by low energy supply and phosphorylation of LKB1 results in an inhibition of mTOR , thus decreasing energy-consuming processes, in particular translation and, thus, cell growth. (sdbonline.org)
Pharmacologic inhibition of GLI1/GLI2 results in decreased hTERT protein expression in human cancer cells. (urat1inhibitor.com)
BDNF/TrkB signaling protects HT-29 human colon cancer cells from EGFR inhibition. (natprobiotech.com)
PARP inhibition and pharmacological ascorbate demonstrate synergy in castration-resistant prostate cancer. (jefferson.edu)
Inhibition of JNK Sensitizes Hypoxic Colon Cancer Cells to DNA-Damaging Agents. (jefferson.edu)
In comparison to monotherapy, CTX + IL-2 significantly limited tumor growth, via tumor cell proliferation inhibition and increased apoptosis. (bvsalud.org)

We also discovered that the invasiveness of metastatic colon cancer cells was alleviated following drug treatment. (spandidos-publications.com)
These findings suggest that formononetin inhibits angiogenesis and tumor cell invasion, and thus support its use in the treatment of advanced and metastatic colon cancers. (spandidos-publications.com)
When targeting cancer cells, both animal and plant proteins may be used in a formulation process to create self-assembled viruses and platforms that can successfully eradicate metastatic cancer cells. (benthamscience.com)
Although these therapeutic regimens achieve somewhat satisfactory local disease control, effective regimens for metastatic CRC patients with liver metastases from colorectal cancer are limited. (hindawi.com)
CCR5 blockage by maraviroc: a potential therapeutic option for metastatic breast cancer. (cancerindex.org)
Paclitaxel enhances the effects of the anti-epidermal growth factor receptor monoclonal antibody ImClone C225 in mice with metastatic human bladder transitional cell carcinoma. (natprobiotech.com)

Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
MMPs have been implicated in these processes and a previous study has suggested that the plasma level of MMP-2 and MMP-9 may be viewed as a non-invasive indicator of invasiveness and metastasis in colon cancer ( 3 ). (spandidos-publications.com)
Bone metastasis is observed in up to 70% of breast cancer patients. (cancerindex.org)
Such cells are capable of tumour initiating, self-renewal, invasion and metastasis, resulting in tumour relapse and resistance. (researchgate.net)

It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)
We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
Enhancement by Nano-Diamino-Tetrac of Antiproliferative Action of Gefitinib on Colorectal Cancer Cells: Mediation by EGFR Sialylation and PI3K Activation. (umassmed.edu)
Colorectal cancer cells are resistant to anti-EGFR monoclonal antibody through adapted autophagy. (natprobiotech.com)

In vivo, NTSR1 mRNA expression was undetectable in superficial differentiated epithelial cells in histological specimens of normal human colonic epithelium, but there was moderate and strong expression in adenomas and adenocarcinomas respectively. (springer.com)

MFE and doxorubicin exert a cytotoxic effect on human colon cancer HT-29 cell line by probably promoting or induction of apoptosis. (cellmolbiol.org)
From these assays, it is concluded that D. viscosa does not possess any detectable cytotoxic effect on epidermal cells from mouse (3T3), and a slight cytotoxic effect against HT-29 tumor cells compared to 5-FU. (edu.pe)
The primary goal of this research was to investigate the apoptotic, autophagic, and cytotoxic effects of cetuximab (CTX), a monoclonal antibody used to treat colorectal cancer. (natprobiotech.com)
Interleukin-2 (IL-2) benefits some cancer patients by promoting the proliferation of cytotoxic effector T cells, but this process is limited by the expansion of regulatory T cells (Tregs). (bvsalud.org)
Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)

A novel angiogenesis inhibitor impairs LoVo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. (rain-tree.com)
In vitro, it was found that adiponectin directly controls malignant potential (cell proliferation, adhesion, invasion and colony formation) and regulates metabolic (AMPK/S6), inflammatory (STAT3/VEGF) and cell cycle (p21/p27/p53/cyclins) signalling pathways in both mouse MCA38 and human HT29, HCT116 and LoVo colon cancer cell lines in a LKB1-dependent way. (nuthealth.org)

In this context, we assess the reliability of the neurotensinergic system to cancer progression as well as the regulation and mechanism of the system in order to determine its potential in colorectal cancer diagnosis, surveillance and screening. (springer.com)
Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)
2 - 4 Expression profiling on a genomic scale led to the classification of the intrinsic subtypes of breast cancer, thus allowing for the prediction of disease progression and patient stratification. (dovepress.com)
Background: Obesity and a high-fat diet are associated with the risk and progression of colon cancer. (nuthealth.org)
OBJECTIVE: Yes-associated protein (Yes-associated protein 1 [YAP1]) is an important oncogene that is related to the pathogenesis and progression of liver cancer. (bvsalud.org)

In this study, we investigated a plausible therapeutic synergism of a triple combination of CBD/CBG, curcumin, and piperine in the colon adenocarcinoma using HCT116 and HT29 cell lines. (frontiersin.org)
Our findings revealed that different genetic backgrounds of HCT116 and HT29 cell lines resulted in divergent responses to the combination treatments. (frontiersin.org)
Triple treatment showed synergism in terms of exhibiting anti-tumorigenic effects by activating the Hippo YAP signaling pathway in the HCT116 cell line. (frontiersin.org)

Updated national referral guidelines for the recognition of cancer were introduced in 2016. (springer.com)

Cormasine is thought to kill tumor cells by direct cell lysis, showing selectivity for cancer cells as opposed to healthy cells via a rhamnose binding protein. (wikipedia.org)

Breast cancer is one of the most common cancers affecting women worldwide. (dovepress.com)

The mechanistic basis for the biological properties of Morus alba flavonoid extract (MFE) and chemotherapy drug of doxorubicin on human colon cancer HT-29 cell line death are unknown. (cellmolbiol.org)
According to the stage of cancer and the degree of the complication, current treatment methods rely on the success of chemotherapy either alone or combined with surgical resection or radiation therapy. (frontiersin.org)
Recent advances in cancer research have seen significant advances in therapies such as chemotherapy and immunotherapy, although both have limitations in effectiveness and toxicity. (benthamscience.com)
Colorectal cancer (CRC) is one of the most frequent cancers, and chemotherapy plays an important role in its treatment. (natprobiotech.com)

In in vitro studies, the addition of NT to human colon cancer cell lines resulted in significantly increased cancer cell growth and the growth of xenografted human colon cancer cells in mice [ 9 ]. (springer.com)
Recently we showed that a single oral dose of Salmonella enterica serovar Typhimurium expressing a truncated human interleukin-2 gene (SalpIL2) is avirulent, immunogenic, and reduces hepatic metastases through increased natural killer cell populations in mice. (weeksmd.com)
This study addresses the effects of two nutraceutical compounds on the expression of COX2 and tumor-associated inflammation in human papillomavirus type 16 (HPV16)-transgenic mice. (iiarjournals.org)
Mice appeared to be more similar to humans than rats to humans in the glucuronidation of EGCG and EGC. (aspetjournals.org)
Salutary effects of adiponectin on colon cancer: in vivo and in vitro studies in mice. (nuthealth.org)
Objective: To investigate the effects of adiponectin on the growth of colorectal cancer in adiponectin-deficient or wild-type-C57BL/6 mice fed a low-fat or high-fat diet. (nuthealth.org)
Proliferation index was calculated as the quantity of Ki67 positive cells divided by the whole amount of cells.Survivin gene deletion in the prostate of male mice was attained utilizing the Cre-Lox P technique. (urat1inhibitor.com)
Epidermal growth factor receptor blockade with C225 plus gemcitabine results in regression of human pancreatic carcinoma growing orthotopically in nude mice by antiangiogenic mechanisms. (natprobiotech.com)

Potential synergistic effects of various combinations including these compounds were tested by measuring cancer cell proliferation and apoptosis. (frontiersin.org)

5 Triple-negative breast cancer is characterized by limited treatment options and frequent local recurrence. (dovepress.com)

A natural anticancer agent taspine targets human topoisomerase IB. (rain-tree.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells. (hindawi.com)
Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
Recently, adenine has been explored for its anticancer property in several types of cancer cell lines. (hindawi.com)
Motilva, V. Anticancer Activities of Meroterpenoids Isolated from the Brown Alga Cystoseira usneoides against the Human Colon Cancer Cells HT-29. (ualg.pt)
It was found that miR-424-5p expression was significantly decreased in liver cancer tissues, revealing its anticancer effect. (bvsalud.org)

Effects of taspine on proliferation and apoptosis by regulating caspase-3 expression and the ratio of Bax/Bcl-2 in A431 cells. (rain-tree.com)
This study investigated the role of miR-424-5p in regulating YAP1 expression and affecting hepatoma cell proliferation and apoptosis. (bvsalud.org)

Generation of inhibitory peptides against bacteria and HT29 human colon cancer cells improved with [pineapple peel powder] supplementation of yogurt. (mdlinx.com)
Our previous findings demonstrated that formononetin initiates growth-inhibitory and pro-apoptotic activities in human colon cancer cells. (spandidos-publications.com)
Subsequent study using multiple human cancer cell lines, including those of the colon, stomach and liver, further indicated the universal growth-inhibitory and pro-apoptotic effects of AST. (spandidos-publications.com)
The ethanol extrac of leaves exhibited a major inhibitory effect compared to different fractions in human colon cancer cells (HT-29). (edu.pe)

Epidermal growth factor receptor blockade with C225 modulates proliferation, apoptosis, and radiosensitivity in squamous cell carcinomas of the head and neck. (natprobiotech.com)
Afatinib vs Placebo as Adjuvant Therapy After Chemoradiotherapy in Squamous Cell Carcinoma of the Head and Neck: A Randomized Clinical Trial. (jefferson.edu)

1 A series of high-throughput methods have changed cancer research and therapy. (dovepress.com)
17-19 Developed by scientists at the USDA Human Nutrition Research Center on Aging at Tufts, the ORAC test is considered one of the most sensitive and reliable methods for measuring the ability of antioxidants to absorb free radicals. (lifeextension.com)

citation needed] Animal studies and in vitro studies showed positive results, however Cham decided to focus his energies on developing the glycoalkaloid mixture, patented as BEC, as a topical cream for non-melanoma skin cancer. (wikipedia.org)
Using animal models, in vivo and in vitro studies have suggested that green tea polyphenols interact with a number molecular targets that are active in cellular processes involved in a range of diseases such as certain cancer (further discussed in Role of green tea in preventing and fighting cancer). (lumminary.com)

Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)

KSO-SFE at 600/40 showed the strongest cytotoxicity towards HT29 with IC 50 of 200 µg/mL. (hindawi.com)
Therefore, this study determined the cytotoxicity of KSO-SFE on colon cancer cells. (hindawi.com)

Relative cell viability was assessed using MTT assays. (wjgnet.com)

Sangre de grado ( Croton palanostigma ) induces apoptosis in human gastrointestinal cancer cells. (rain-tree.com)
We may conclude that CTX induces apoptosis and autophagy in HT-29 KRK cells through raising the expression of p21, p27 , and p57 genes, as well as ATG4A, LC3A , and BECN1 genes. (natprobiotech.com)

Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. (wjgnet.com)
Cell apoptosis was detected by flow cytometry. (bvsalud.org)

Our results indicated that AST inhibits cell proliferation through phase-specific cell cycle arrest and caspase-dependent apoptosis. (spandidos-publications.com)
Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)
10 TRPV1 agonist, capsaicin, is capable of inducing apoptosis 11 , 12 and inhibiting cancer cell growth by cell cycle arrest in many different types of cancer, for example, osteosarcoma, colon, and pancreatic cancer cells, while normal cells remained unharmed. (dovepress.com)
Wu X, Song M, Qiu P, Li F, Wang M, Zheng J, Wang Q, Xu F, Xiao H. A metabolite of nobiletin, 4'-demethylnobiletin and atorvastatin synergistically inhibits human colon cancer cell growth by inducing G0/G1 cell cycle arrest and apoptosis. (umassmed.edu)
Induction of cell cycle arrest and apoptosis with downregulation of Hsp90 client proteins and histone modification by 4? (umassmed.edu)

Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses. (hindawi.com)
Transient receptor potential (TRP) channels contribute to the regulation of intracellular calcium, which can promote cancer hallmarks in cases of dysregulation of gene transcription and calcium-dependent pro-proliferative or anti-apoptotic mechanisms. (dovepress.com)
At 50 μg mL -1 , the extract had potently induced apoptotic cell death of HCT-116 colon cancer cell line by inducing caspases 3 and 7 activity. (scialert.net)
These results showed that n-hexane extract of S. koetjape possess both anti-angiogenic and apoptotic properties on colon cancer cell lines making it a good candidate for further studies. (scialert.net)
The process of systemically destroying damaged cell is called apoptosis and is signaled for and controlled by apoptotic proteins. (lumminary.com)

The master kinase LKB1 is a key regulator of several cellular processes, including cell proliferation, cell polarity and cellular metabolism. (sdbonline.org)

Although, non-psychoactive cannabidiol (CBD) and cannabigerol (CBG) display antineoplastic effects by repressing tumor growth and angiogenesis both in cell line and animal models, their use as chemotherapeutic agents is awaiting further investigation. (frontiersin.org)
A novel taspine analog, HMQ1611, inhibits growth of non-small cell lung cancer by inhibiting angiogenesis. (rain-tree.com)
In the present study, we aimed to further examine the potential of formononetin in controlling angiogenesis and tumor cell invasiveness in human colon cancer cells and tumor xenografts. (spandidos-publications.com)

RNA sequencing was performed on Hematopoietic Stem and Progenitor Cells, and DNA damage was assayed using immunofluorescence staining and comet electrophoresis. (bvsalud.org)

These images are of human HT29 colon cancer cells, a cell line that has been widely used for the study of many normal and neoplastic processes. (broadinstitute.org)
used the HT29 cell line to screen 4,903 shRNAi constructs (targeting 1,028 human genes) in order to find mitotic regulators. (broadinstitute.org)
The effect of doxorubicin and flavonoid extract on colon cancer HT-29 cell line death and identification of APC gene expression and PARP concentration of HT-29 cell line were investigated. (cellmolbiol.org)
The results showed that flavonoid extract and doxorubicin induce a dose dependent cell death in HT-29 cell line. (cellmolbiol.org)
To investigate the effect of gambogic acid (GA) on apoptosis in the HT-29 human colon cancer cell line. (wjgnet.com)
Proanthocyanidin derived from the leaves of Vaccinium virgatum suppresses platelet-derived growth factor-induced proliferation of the human hepatic stellate cell line LI90. (rain-tree.com)
This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)

Cell Oncol (Dordr). (cancerindex.org)

Adenine is involved in a variety of cell biological processes and has been explored for pharmacological uses. (hindawi.com)
Mutation of cell-cycle regulators biological and clinical implications for human neoplasia. (natprobiotech.com)
Herein, we demonstrated the biological effects and mechanisms of asymmetrical loss in hematopoietic stem cells (HSCs). (bvsalud.org)

Apart from these, the results from clinical studies have shown that Astragalus polysaccharides counteract against the side-effects of chemotherapeutic drugs, including a significant attenuation of myelosuppression in cancer patients ( 7 ). (spandidos-publications.com)
These data indicate that AST may be an effective chemotherapeutic agent in colon cancer treatment, and may also be used as an adjuvant in combination with other orthodox chemotherapeutic drugs to reduce the side-effects of the latter compounds. (spandidos-publications.com)
Differential roles of RIPK1 and RIPK3 in TNF-induced necroptosis and chemotherapeutic agent-induced cell death. (umassmed.edu)

Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)

To understand the role of autophagy in the pathogenesis of glial tumors in vivo, an established Drosophila melanogaster model of glioma was used based on overexpression in larval glial cells of an active human EGFR and of the PI3K homolog Pi3K92E /Dp110. (sdbonline.org)
Anti-EGFR antibody sensitizes colorectal cancer stem-like cells to Fluorouracil-induced apoptosis by affecting autophagy. (natprobiotech.com)
EGFR blockade by cetuximab alone or as combination therapy for growth control of hepatocellular cancer. (natprobiotech.com)

It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)

These benefits show hTERT to be a transcriptional target of the HH signaling pathway and identify a formerly unfamiliar position of the HH/GLI axis in regulating the replication possible of cancer cells. (urat1inhibitor.com)

Indeed, cranberry juice may prevent the adherence of bacteria to uroepithelial cells, and suppress inflammatory responses due to infection. (mdlinx.com)

We abrogated the HH/GLI signaling axis in multiple human cancer cell lines with pharmacological Figure 1. (urat1inhibitor.com)

Among its different constituents, Astragalus polysaccharides have been most widely studied particularly for their immunopotentiating properties, such as stimulation of murine B cell proliferation and cytokine production ( 6 ). (spandidos-publications.com)

Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS. (umassmed.edu)
Role of p27(Kip1) in human intestinal cell differentiation. (natprobiotech.com)

A: Mushroom extracts have gained popularity as remedies for a variety of ailments, including seasonal allergies, insomnia, cancer, the common cold, and inflammation. (todaysdietitian.com)

whereas human epidermal growth factor receptor 2 (HER2)-positive breast cancers can be targeted by specific drugs, the triple-negative breast cancer phenotype is defined by chromosomal instability and low expression levels of estrogen receptor (ER) alpha, progesterone receptor (PR), and HER2, rendering treatment rather difficult. (dovepress.com)

It is part of the membranes (outer coverings) of all cells, and plays a role in helping the liver process fats as well as contributing to the function of muscles and nerves. (empowher.com)
Adenine is a purine derivative which is synthesized in the liver in humans. (hindawi.com)
Liver cancer HCCLM3 and MHCC97-L cells and normal liver HL-7702 cells were cultured in vitro to compare expression levels of miR-424-5p and YAP1. (bvsalud.org)
RESULTS: Compared with normal liver tissue, miR-424-5p expression was significantly decreased, while YAP1 mRNA and protein levels were obviously upregulated in liver cancer tissues, which were related to the clinical stage. (bvsalud.org)

Our findings suggest that adenine inhibits the growth of colon cancer cells. (hindawi.com)
These findings expose a new operate of HH signaling in increasing the replicative ability of most cancers cells. (urat1inhibitor.com)

EGCG interacts with important cell cycle regulatory proteins, such as cyclins D1 and E and their cyclin-dependent kinases 2,4 and 6. (lumminary.com)

Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. (cancerindex.org)
Furthermore, it is possible to say that reducing EGF and KRAS gene expression inhibits cancer cell growth. (natprobiotech.com)

The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)

Cancer Lett. (wikipedia.org)

HT29 Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)

Earlier detection of colorectal cancer (CRC) results in improved survival. (springer.com)
Adenine improves the survival of rat Purkinje cells and enhances the storage of erythrocyte in whole blood [ 6 - 8 ]. (hindawi.com)
Oral cancer has a poor survival rate despite comprehensive therapy. (researchgate.net)

EGCG is especially important and active in many essential cellular pathways including the cell cycle. (lumminary.com)

Cell Death Dis. (rain-tree.com)

Myeloid immune cells, specifically tumor-associated macrophages (TAMs), may represent potential novel therapeutic targets in HCC, complementing current ablative or immune therapies. (cancerindex.org)

The infiltration of CD8+ T cells in tumor tissues was significantly increased in the CTX + IL-2 group. (bvsalud.org)

The tumor size and the number of proliferating cells were reduced in the tumor tissues obtained from the formononetin-treated group. (spandidos-publications.com)
Next-generation sequencing analyses revealed the expression of TRPV1 in several native breast cancer tissues, which was subsequently validated via reverse transcriptase-polymerase chain reaction. (dovepress.com)
In conclusion, the current study revealed the expression profiles of human TRP channels in 60 different breast cancer tissues and cell lines and furthermore validated the antitumor activity of TRPV1 against SUM149PT breast cancer cells, indicating that activation of TRPV1 could be used as a therapeutic target, even in the most aggressive breast cancer types. (dovepress.com)

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Inhibition11

Metastatic6

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Colorectal cancer cells4

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HCT1163

20161

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Chemotherapy4

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Inhibitory4

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Apoptosis was detected2

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