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  • levels of hsp90
  • At low levels of hsp90, ligand-dependent activation of the chimeric dioxin receptor construct was almost completely inhibited, whereas the activity of a similar chimeric construct containing the structurally related Arnt factor was not affected. (pnas.org)
  • molecular
  • With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)
  • Using a sophisticated methodology, a team led by Professor Thorsten Hugel, head of the Department for Molecular Machines at the TU Muenchen and member of the Excellence Cluster Nanosystemns Initiative Munich (NIM), has now managed to observe this reaction in detail for the first time - step for step in an Hsp90 molecule, P23 protein and ATP. (phys.org)
  • Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. (mybiosource.com)
  • Wee1-Hsp90 inhibitor combination treatment: molecular therapy with potentially broad applicability. (biomedsearch.com)
  • These observations support that HSP90α, HA, and, PGE2 could be potential molecular markers for prediction of delayed wound healing due to pressure. (nih.gov)
  • Here we describe two functional screens for proteins required for the invasion of fibrosarcoma cells that identified the molecular chaperone heat shock protein 90 (hsp90). (semanticscholar.org)
  • The transcriptional response included differential expression of genes encoding stress response components such as the antioxidant network and molecular chaperones, cellular components such as core photosynthesis machinery, integral light-harvesting protein complexes and enzymes such as fatty acid desaturases. (frontiersin.org)
  • actin
  • After 18 hrs incubation, cells were lysed with the Laemmli buffer added with 7 mM β-mercaptoethanol, and whole lysates analysed in Western blotting for total and phosphorylated forms of ERK1/2 (A) and Akt proteins (B). Membranes were then probed for β actin for normalization of protein content. (nih.gov)
  • scaffold
  • As a whole, our data indicate that Hsp90 may serve as scaffold to keep the CB2 receptor and its signaling components, including Gα i2 , in proximity, thus facilitating CB2-mediated signaling to cell migration through the G i -Rac1 pathway. (aspetjournals.org)
  • A purine scaffold Hsp90 inhibitor destabilizes BCL-6 and has specific antitumor activity in BCL-6-dependent B cell lymphomas. (nih.gov)
  • recombinant
  • Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
  • Small volumes of HSP90AA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
  • Peptides
  • Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
  • Mutation
  • Similarly, K287Q acetylation mimicking mutation repressed Hsp90α1 function in myosin thick filament organization. (zfin.org)
  • The system relies on detection of Lac + revertants using MMR-proficient or MMR-deficient E. coli strains carrying a lac +1 frameshift mutation expressing hMLH1 proteins. (biochemj.org)
  • Conversely, expression of proteins encoded by alleles deriving from hereditary-non-polyposis-colon-cancer (HNPCC) families decreases mutation rates, depending on the specific amino acid substitutions. (biochemj.org)
  • transcriptional
  • Here we have replaced the bHLH motif of the dioxin receptor with a heterologous DNA-binding domain to create fusion proteins that mediate ligand-dependent transcriptional enhancement in yeast (Saccharomyces cerevisiae). (pnas.org)
  • expression
  • To investigate the role of hsp90 in dioxin signaling we have studied receptor function in a yeast strain where hsp90 expression can be down-regulated to about 5% relative to wild-type levels. (pnas.org)
  • Cid C, Regidor I, Poveda PD, Alcazar A (2009) Expression of heat shock protein 90 at the cell surface in human neuroblastoma cells. (usp.br)
  • Thus, a growing amount of evidence has connected the action of Hsp90 to post-translational modifications of soluble nuclear factors, DNA, and histones, which epigenetically affect gene expression upon the onset of an unfriendly environment. (biochemsoctrans.org)
  • The protective role of Hsp90 not only elevates Bcl-2/Bax and Bcl-xL/Bax expression and attenuates cleaved caspase-3 expression via down-regulating membrane TLR-4 and ErbB2 receptors and then activating their downstream PI3K/Akt and ERK1/2 pathways, but also enhances the paracrine effect of MSCs. (biomedsearch.com)
  • Expression of heat shock proteins in brain tumors. (semanticscholar.org)
  • Here we show that even sublethal concentrations of DNA-damaging drugs, such as etoposide and cisplatin, induce the expression of autophagy-related protein 5 (ATG5), which is both necessary and sufficient for the subsequent induction of mitotic catastrophe. (nih.gov)
  • A simple genetic system has been developed to test the effect of over-expression of wild-type or mutated human MutL homologue 1 (hMLH1) proteins on methyl-directed mismatch repair (MMR) in Escherichia coli . (biochemj.org)
  • We report that expression of wild-type hMLH1 protein causes an approx. (biochemj.org)
  • machinery
  • Four-colour FRET reveals directionality in the Hsp90 multicomponent machinery, C. Ratzke, B. Hellenkamp, T. Hugel, Nature Communications 5, Article number: 4192. (phys.org)
  • depletion
  • Since a proteosome inhibitor only partially rescued PU-H71 depletion of Bcl6 (Fig. 2d), we wondered whether Hsp90 might also block degradation of Bcl6 mRNA. (nih.gov)
  • Function
  • Systematic Mutant Analyses Elucidate General and Client-Specific Aspects of Hsp90 Function. (umassmed.edu)
  • More recently, interest in Hsp90 has expanded to include an apparently mechanistically distinct function. (aspetjournals.org)
  • In contrast, K206R and K608R hypomethylation mimicking mutations had not effect on Hsp90α1 function in thick filament organization. (zfin.org)
  • Targets
  • G protein-coupled receptors (GPCRs) are of vital importance for cellular functions, and they are important targets for drug discovery ( Gao and Jacobson, 2006 ). (aspetjournals.org)
  • thereby
  • Using this approach, the Munich researchers successfully demonstrated in detail that the P23 protein strengthens ATP bonding, thereby significantly increasing the amount of energy exploited. (phys.org)
  • They also showed that the two substances bond with Hsp90 this effectively only as a team, thereby allowing ATP splitting to be used so successful. (phys.org)
  • The mutator phenotype was due to the ability of hMLH1 protein to interact with bacterial MutL and MutS proteins, thereby interfering with the formation of complexes between MMR proteins and mismatched DNA. (biochemj.org)
  • role
  • Crucial role of HSP90 in the Akt-dependent promotion of angiogenic-like effect of glucose-regulated protein94 (Grp94)-IgG complexes. (nih.gov)
  • Heat shock protein 90α plays a key role in myosin folding and thick filament assembly in muscle cells. (zfin.org)
  • interact
  • To determine exactly how the components Hsp90, P23 and ATP interact with each other, the biophysicists observed the positions and bonding sequences of the individual molecules over a span of several minutes. (phys.org)
  • investigate
  • In the near future the biophysicists want to investigate in detail how Hsp90 uses the exploited energy. (phys.org)
  • crucial
  • Disruption of the CB2/Hsp90 interaction by treatment with GA or reducing Hsp90 levels with specific short interfering RNAs markedly inhibited 2-AG-induced cell migration, demonstrating that Hsp90 is crucial for 2-AG-induced cell migration. (aspetjournals.org)
  • specific
  • This relationship was also supported by the presence of two indels, specific insertions or deletions within conserved protein regions , one of which encompasses a charged domain . (jbsdonline.com)
  • indicate
  • Taken together these results indicate that CFTR turnover is initiated by calpain activation, which is induced by an increased Ca 2+ influx and, following internalization of the cleaved channel protein, and completed by the lysosomal proteases. (biochemj.org)