Hscs reconstitute the hematopoietic hematopoietic stem cell transplantation bone marrow transplantation. Medical search. Frequent questions

Stem and progenitor cells can be taken from the pelvis, at the iliac crest, using a needle and syringe. (wikipedia.org)
Single-cell transcriptomic analysis confirmed inhibition of lineage-committed progenitor cells in physioxic cultures. (stanford.edu)
Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
The Gerbaulet group studies the biology of hematopoietic stem and progenitor cells in their native environment. (digs-bb.de)
For decades, hematopoietic stem and progenitor cells have been studied primarily through such transplantation-based assays. (digs-bb.de)
Preconditioning of recipients by lethal irradiation or chemotherapy forces transplanted hematopoietic stem and progenitor cells to realize their maximum potential, but this does not necessarily reflect their fate and behavior in the native bone marrow environment. (digs-bb.de)
To investigate the proliferation and differentiation of hematopoietic stem and progenitor cells in their physiologic environment, we use state-of-the-art mouse models. (digs-bb.de)
At steady-state, HSC infrequently divide and slowly but continuously spawn rapidly dividing progenitor cells. (digs-bb.de)
We want to understand how the hierarchical organization of mature blood cell regeneration from stem and progenitor cells shapes the accumulation of somatic mutations and hence the risk of developing blood cancer. (digs-bb.de)
The key difference between hematopoietic stem cells and progenitor cells is, Think of the hematopoietic stem cell as the ultimate multitasker. (thenoveldifference.com)
On the other hand, progenitor cells are like specialists. (thenoveldifference.com)
Hematopoietic stem cells are the all-in-one package, while progenitor cells are like specialists who focus on their specific roles. (thenoveldifference.com)
Hematopoietic stem cells give rise to progenitor cells, which, in turn, differentiate into specific blood cell lineages. (thenoveldifference.com)
What are Progenitor Cells? (thenoveldifference.com)
Progenitor cells, also known as precursor cells, are a type of cell that falls somewhere between stem cells and fully differentiated, specialized cells in terms of their differentiation potential. (thenoveldifference.com)
Progenitor cells are more specialized than stem cells. (thenoveldifference.com)
They have already committed to a particular differentiation pathway, meaning they are destined to become a specific type of cell, such as a red blood cell, white blood cell, or platelet in the case of hematopoietic progenitor cells. (thenoveldifference.com)
While progenitor cells can divide and replicate, their proliferation potential is more restricted compared to stem cells. (thenoveldifference.com)
Progenitor cells play a crucial role in tissue repair and regeneration. (thenoveldifference.com)
Progenitor cells are often located within specific tissues, close to where they will ultimately function. (thenoveldifference.com)
For example, hematopoietic progenitor cells are found in the bone marrow, as this is where blood cell production occurs. (thenoveldifference.com)
Progenitor cells are being explored for various therapeutic applications, including tissue engineering and regenerative medicine. (thenoveldifference.com)
Progenitor cells are partially specialized cells that serve as intermediaries between stem cells and fully differentiated cells. (thenoveldifference.com)
Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
Blood cells are produced within bone marrow by specialized stem cells and progenitor cells. (elifesciences.org)

In vertebrates, the vast majority of hematopoiesis occurs in the bone marrow and is derived from a limited number of hematopoietic stem cells that are multipotent and capable of extensive self-renewal. (wikipedia.org)
In experimental settings, the ability of human HSCs to reconstitute hematopoiesis is measured by transplantation into genetically immunocompromised mice (e.g. (stemcell.com)
However, more primitive cells capable of reconstituting hematopoiesis did not express LFA-1. (elsevierpure.com)
Similar to tissue-resident macrophages, mast cells are of dual ontogeny and are generated during both fetal and adult ("definitive") hematopoiesis. (digs-bb.de)
The generation of HSCs has been a long-standing goal of stem cell biologists working in the field of developmental hematopoiesis. (utoronto.ca)
The concept of the existence of an HSC that is capable of reconstituting hematopoiesis in vivo was first introduced more than 60 years ago, when Jacobson et al. (longdom.org)
Hematopoiesis is the process of blood cell formation. (thenoveldifference.com)
To address this knowledge gap, we developed an in vivo hematopoietic stem cell (HSC)-based large-scale CRISPR knockout screening platform to enable the genetic interrogation of hematopoiesis and broad aspects of immune cell function in vivo. (biorxiv.org)
Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
Mouse hematopoiesis was thought to develop initially in the yolk sac, where primitive hematopoietic development occurs [ 4 ]. (biomedcentral.com)

The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (wikipedia.org)
We hypothesize that the continuous but rare differentiation of quiescent HSCs into rapidly dividing progenitors limits mutation accumulation in the hematopoietic system. (digs-bb.de)
Early in murine embryonic development, hematopoietic progenitors can be found in the para-aortic splanchnopleura region on embryonic day 7 (as depicted in Figure 1 A), but these progenitors are not LT reconstituting although they do possess lymphoid potential [ 11 - 13 ]. (biomedcentral.com)
These more advanced hematopoietic progenitors isolated from the aorta-gonad-mesonephros (AGM) region at embryonic day 9.5 to 10 can give rise to lymphoid lineages and are LT reconstituting definitive HSCs, but since this time point is after the establishment of the circulatory system in the embryo their source was difficult to determine [ 13 ]. (biomedcentral.com)
Cumano and colleagues' approach was to culture explants containing hematopoietic progenitors isolated from the yolk sac and the embryo proper before the onset of circulation, and to determine their LT reconstitution potential [ 10 ]. (biomedcentral.com)
In a murine competitive reconstitution model, Mybl2 knockdown by RNAi to 20-30% of normal levels in multipotent hematopoietic progenitors resulted in clonal dominance of these 'sub-haploinsufficient' cells, which was reflected in all blood cell lineages. (elifesciences.org)
We conclude that downregulation of MYBL2 activity below levels predicted by classical haploinsufficiency underlies the clonal expansion of hematopoietic progenitors in a large fraction of human myeloid malignancies. (elifesciences.org)

Myeloid and lymphoid lineages both are involved in dendritic cell formation. (wikipedia.org)
This experimental paradigm revealed the existence of a rare population of somatic stem cells that can stably reconstitute all blood lineages lifelong. (digs-bb.de)
The future direction of the study of hematopoietic stem cells will probably make use of pluripotent stem cells to yield specific immune cell lineages and eventual clinical applications. (biomedcentral.com)
The cells gave rise to all major blood lineages, including red blood cells, B cells, and T cells. (nih.gov)

Hematopoietic stem cells are found in the bone marrow of adults, especially in the pelvis, femur, and sternum. (wikipedia.org)

Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. (stanford.edu)
At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)
The term stem cells dates back to the late 1800s at which time it referred to the fertilized egg, a pluripotent cell able to give rise to all of the complex cell types that comprise our body. (pas.va)
Induced pluripotent stem cells (iPSC) can now be generated from skin or blood of mice or humans by overexpressing four key transcription factors. (pas.va)
Two new studies by NIH-funded research teams bring us closer to achieving this feat. In the first study, researchers developed a biochemical "recipe" to produce HSC-like cells from human induced pluripotent stem cells (iPSCs), which were derived from mature skin cells. (nih.gov)
Bypassing the pluripotent state, in which stem cells are still capable of producing several different cell types, the researchers showed that expression of only four transcription factors was sufficient to convert the adult endothelial cells into long-lasting HSCs. (nih.gov)

Stem cells are not specialized and the process of their specialization is called differentiation. (benthamscience.com)
Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
moreover, they also promote the hematopoietic differentiation of mouse ESCs and favor the development of definitive HSCs from pre-HSCs in the mouse aorta-gonad-mesonephros (AGM) region [ 8 ]. (biomedcentral.com)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)

Hematopoietic stem cells can replenish all blood cell types (i.e., are multipotent) and self-renew. (wikipedia.org)
We recently unraveled an alternative pathway of thrombopoiesis, in which HSCs directly differentiate into megakaryocytes, bypassing the classical multipotent progenitor intermediates (Morcos, Li, Munz et al. (digs-bb.de)
Hematopoietic stem cells (HSCs) are a type of multipotent stem cell found primarily in the bone marrow and, to a lesser extent, in peripheral blood. (thenoveldifference.com)

We have tested the feasibility of using the granulocyte colony-stimulating factor (G-CSF), which is known to mobilize hematopoietic stem cells (HSCs) from the bone marrow into the peripheral blood, as a therapeutic agent for AD. (silverchair.com)
Our data indicate that, also in this transplantation setting, NK cells are the first lymphoid population detectable in peripheral blood. (ashpublications.org)

While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)

Allogeneic HSC transplantation (HSCT) is used clinically as a curative therapy for a range of hematolymphoid diseases, but remains a high-risk therapy due to potential side effects including poor graft function and graft-vs-host disease (GvHD). (stanford.edu)
Furthermore, we provide evidence that HSC-selective ex vivo cultures deplete GvHD-causing T cells and that this approach can be combined with genotoxic-free antibody-based conditioning HSCT approaches. (stanford.edu)
Worldwide, 50,000 hematopoietic stem cell transplant (HSCT) procedures are undertaken annually to replace a person's blood-forming cells - hematopoietic stem cells (HSCs) - to treat blood disorders and cancers, yet two-thirds of patients who need HSCT lack matched donor tissue. (utoronto.ca)
In addition to limiting HSC numbers, HSCT is inefficient, as many HSC do not get to the relevant areas in the body and/or die following transplantation. (utoronto.ca)
Since then, allogeneic hematopoietic stem cell transplantation (HSCT) has evolved to become a frequently used and effective therapy for many hematologic malignancies. (longdom.org)

Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
Hematopoietic stem cells (HSCs) are at the top of the hematopoietic hierarchy and can differentiate into all types of mature blood cells throughout life. (digs-bb.de)
Bone marrow is the place for the production of the hematopoietic cells which differentiate in to three groups: red blood cells, white blood cells and platelets. (longdom.org)
They differentiate into very specific cells. (thenoveldifference.com)
HSCs have the remarkable ability to differentiate into a wide range of specialized blood cells. (thenoveldifference.com)

HSCs were first identified by transplantation, in which recipient mice were preconditioned with ionizing radiation and reconstituted with donor bone marrow. (digs-bb.de)
Despite advances in using umbilical cord blood, donor material remains restricted by limited stem cells and the lack of ethnic diversity to provide sufficiently matched material. (utoronto.ca)
The Allogeneic bone-marrow transplantation represents the only curative treatment and requires a compatible donor HLA. (longdom.org)
We initially search a potential donor within the siblings of the patient by determining HLA type, true genetic identity card of the cells. (longdom.org)
Bone marrow transplants offer a way to cure leukemia, sickle cell disease, and a variety of other life-threatening blood disorders.There are two major problems, however: One is many patients don't have a well-matched donor to provide the marrow needed to reconstitute their blood with healthy cells. (nih.gov)
A much more powerful option would be to develop a means for every patient to serve as their own bone marrow donor. (nih.gov)

Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
We found that when we transplant endothelial cells with this limited number of bone marrow cells the mice survived and recovered faster," said lead author Dr. Michael Poulos , an instructor of regenerative medicine at Weill Cornell Medicine. (cornell.edu)
Hematopoietic stem cells are pivotal in maintaining the body's blood cell supply, and their versatility makes them invaluable in both medical treatments and scientific research related to blood disorders and regenerative medicine. (thenoveldifference.com)

The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
In a study published online on Oct. 16 and appearing in the November issue of The Journal of Clinical Investigation, Dr. Butler and his colleagues report that the age of blood vessel cells in the bone marrow strongly influences the function of blood stem cells. (cornell.edu)
This approach is appealing because endothelial cell infusions can potentially supplement a number of currently available bone marrow transplantation therapies to improve the overall clinical outcomes in both young and aged patients. (cornell.edu)
This sort of transplantation is most commonly performed in three clinical scenarios: (1) to treat malignancies such as cancer in which the immune system is weakened or impaired, (2) to replace an absent or poorly functioning hematopoietic or immune system, and (3) to treat genetic diseases caused by insufficient gene expression by performing a transplantation from donors with normal gene expression. (sagepub.com)
Currently, allogeneic HSC transplantation has been widely used in a clinical setting, yet allogeneic transplantation often leads to graft versus host disease (GVHD) [ 1 ]. (biomedcentral.com)

There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
The methods proposed are cutting-edge and no other team in the world has this combination of technologies, approaches and expertise to make advances in hematopoietic cell therapies. (utoronto.ca)
Stem cell therapies, once a dream, are now becoming a reality. (pas.va)

If one of the brothers and sisters is compatible, we speak about geno-identical allogeneic transplantation having the advantage of reducing the complications post-transplantation. (longdom.org)
By 6 months post-transplantation, the reconstituted mice had developed a clonal myeloproliferative/myelodysplastic disorder originating from the cells with aberrantly reduced Mybl2 expression. (elifesciences.org)

Source material for hematopoietic (bone marrow) transplantation is in great demand as at least 20,000 allogeneic transplants are performed each year. (utoronto.ca)
These observations lay a foundation for developing strategies to improve bone marrow transplants and blood cancer treatment regimens in an aging population. (cornell.edu)
More than 50,000 stem cell transplants are performed each year worldwide. (sagepub.com)

Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. (stanford.edu)
Leukocyte function-associated antigen-1 (LFA-1) (CD11a/CD18) was expressed on some populations of hematopoietic cells and contributed to the proliferation by interacting with stromal cells. (elsevierpure.com)

In this case we will speak about pheno-identical allogeneic transplantation. (longdom.org)

The very first hematopoietic stem cells during (mouse and human) embryonic development are found in aorta-gonad-mesonephros region and the vitelline and umbilical arteries. (wikipedia.org)
As reported in Nature , both teams took advantage of earlier evidence showing that HSCs are formed during embryonic development from budding endothelial cells in the aorta. (nih.gov)

In vertebrates, the very first definitive HSCs arise from the ventral endothelial wall of the embryonic aorta within the (midgestational) aorta-gonad-mesonephros region, through a process known as endothelial-to-hematopoietic transition. (wikipedia.org)

Another CFU, the colony-forming unit-spleen (CFU-S), was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. (wikipedia.org)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
Shortly thereafter, it was demonstrated that intravenous injection of BM cells also provided radioprotection of lethally irradiated mice. (longdom.org)
Transplantation of young blood vessel cells into older mice makes their aged stem cells take on the characteristics of young stem cells. (cornell.edu)
Transplanting young blood vessel cells into older mice can make their aged stem cells take on the characteristics of young stem cells, leading to healthier blood systems and promoting better recovery from cancer treatment side effects, according to new research from Weill Cornell Medicine. (cornell.edu)
When transplanted back into mice, these stem cells grew and functioned better and created healthy blood systems. (cornell.edu)
The investigators then transplanted young blood vessel endothelial cells into mice that had been given a strong dose of whole body radiation. (cornell.edu)
They noted that the blood systems in the mice recovered quickly, indicating that the young endothelial cells boosted the function of the blood stem cells. (cornell.edu)
In another experiment, mice received radiation therapy, similar to what patients undergo prior to bone marrow transplant. (cornell.edu)
Dr. Butler's team then transplanted very low numbers of bone marrow cells into mice, some of which also received young endothelial cells. (cornell.edu)
Further studies in mice by Cumano and colleagues sought to address the issue of an anatomical origin of the long-term (LT) reconstituting hematopoietic stem cell (HSC) found in adult animals [ 10 ]. (biomedcentral.com)
Moreover, injection of modified cells from these animals into healthy mice also induced symptoms in the recipients. (elifesciences.org)
When these HSCs were transplanted into mice, they fully reconstituted the animals' blood systems with healthy red and white blood cells. (nih.gov)
In the parallel study, Raphael Lis and Shahin Rafii from Weill Cornell Medicine, New York, began with readily accessible endothelial cells taken from the organs of adult mice. (nih.gov)
Daley's team matured those human HSC-like cells by transplanting them directly into the bone marrow of living mice. (nih.gov)
Lis and Rafii instead grew the emerging mouse HSCs on a layer of endothelial cells in a dish, where they acquired the attributes of fully functional HSCs, and later were transplanted into mice. (nih.gov)
In the mice, stem cells generated using both approaches took up residence in the bone marrow as expected. (nih.gov)
Importantly, Lis and Rafii found no evidence of cancer for many months after mice were transplanted with the lab-derived HSCs. (nih.gov)

Similarly, a reduction in tacrolimus dose and dosing rate of recurrence from 2?mg twice daily to 0.5?mg every 7 days is expected to maintain Ctrough levels within the range observed before initiation of 3D treatment at 12 months after transplantation. (informationalwebs.com)
Indeed, in a group of patients, a relevant fraction of NK cells expressed a mature phenotype characterized by the KIR + NKG2A − signature 3-6 months after transplantation. (ashpublications.org)

Phenotypic and functional heterogeneity of human NK cells developing after umbilical cord blood transplantation: a role for human cytomegalovirus? (ashpublications.org)
In this study, we analyzed the development of NK cells after intrabone umbilical cord blood (CB) transplantation in 18 adult patients with hematologic malignancies. (ashpublications.org)
Our data support the concept that cytomegalovirus infection may drive NK-cell development after umbilical CB transplantation. (ashpublications.org)

Ex vivo HSC expansion has been suggested as an approach to improve hematopoietic reconstitution from low-cell dose grafts. (stanford.edu)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)

This team, which brings together expertise in stem cell biology, cell biology, developmental signalling pathways, and genetic screens, aims at improving methods to achieve generation of HSC. (utoronto.ca)

Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
Hematopoietic transplantation refers to the therapeutic procedure that involves the introduction of hematopoietic stem cells into a patient to reconstitute his or her immune system. (sagepub.com)
The biology and therapeutic application of mesenchymal cells. (edu.au)

Moreover, NR4A1 deficiency made HT29 xenograft tumors sensitive to necroptotic cell death in vivo. (bvsalud.org)
Long-term physioxic expansion also afforded culture-based ex vivo HSC selection from whole bone marrow, spleen, and embryonic tissues. (stanford.edu)
The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)

Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
This initial wave of fetal hematopoietic development appears to have short-term reconstituting function, as demonstrated by Dieterlen-Lievre using chicken-quail chimeras [ 6 ]. (biomedcentral.com)

Hematopoietic stem cells give rise to different types of blood cells, in lines called myeloid and lymphoid. (wikipedia.org)
Lymphoid cells include T cells, B cells, natural killer cells, and innate lymphoid cells. (wikipedia.org)

Only severe myeloablative perturbations including high dose ionizing radiation or chemotherapy robustly activate HSCs. (digs-bb.de)
In addition, their aged blood stem cells make it hard for them to recover after cancer treatments like chemotherapy and radiation therapy that destroy blood cells. (cornell.edu)
Her innovative research has already led to several discoveries, such as a novel strategy to protect normal Haematopoietic Stem Cells from chemotherapy or radiation damage. (edu.au)

Hematopoietic stem cells constitute 1:10,000 of cells in myeloid tissue. (wikipedia.org)
However, tissue rejection following ESCs derivatives transplantation greatly hinders its application. (benthamscience.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
The hematopoietic system is the most regenerative mammalian tissue. (digs-bb.de)
When specific tissue or organ damage occurs, these cells can rapidly generate new cells of the desired type to facilitate the healing process. (thenoveldifference.com)
They are committed to a specific cell lineage but retain the ability to generate a limited range of related cell types, making them essential for tissue repair, regeneration, and potential medical interventions. (thenoveldifference.com)
embryonic stem cells , resulting from the early divisions of the egg, characterized by their "pluripotency", i.e. the capacity, that they share with the egg cell itself, to produce all the cell types found in the adult organism, and the tissue-specific stem cells present in the tissues and organs of the adult. (pas.va)
These adult stem cells are highly specialized and can only produce the tissue in which they reside. (pas.va)
In addition, there has been remarkable progress in developing approaches to stimulate these tissue-resident stem cells in situ in the tissue. (pas.va)
Importantly, Rafii's team showed they could infuse the lab-derived HSCs, nurtured on the endothelial tissue, directly into a mouse, just as a person would now receive a bone marrow transplant. (nih.gov)

Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
Despite extensive efforts in this area over the past two decades, it is not yet possible to derive HSCs in vitro. (utoronto.ca)
Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)

As people age, their HSCs age as well, leading to greater numbers of poorly-functioning blood cells, a decline in the ability to fight infections, and a predisposition to blood cancers like acute myeloid leukemia (AML), which is characterized by an excess of immature blood cells. (cornell.edu)
They ultimately narrowed down the recipe to a combination of seven transcription factors that were sufficient to convert the iPSC-derived endothelial cells to immature HSC-like cells. (nih.gov)
Both groups relied on external signals to encourage the immature HSCs or HSC-like cells to mature into self-renewing stem cells that no longer needed any assistance from the researchers to continue growing and producing various types of blood cells. (nih.gov)
In other patients, most NK cells maintained an immature phenotype even after 12 months. (ashpublications.org)

A small number of Hematopoietic stem cells can expand to generate a very large number of daughter Hematopoietic stem cells. (wikipedia.org)
Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
These cells are committed to developing into a specific type of cell but still retain the capacity to proliferate and generate a limited range of related cell types. (thenoveldifference.com)

Arrow in first panel points to an endothelial cell induced to become hematopoietic stem cell (HSC). (nih.gov)

What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)

Here, they reside in specialized niches where they remain quiescent until needed to replenish and repair the blood cell population. (thenoveldifference.com)
However, these adult stem cells have more restricted potential - they are specialized to replenish, rejuvenate, and repair the tissues in which they reside. (pas.va)

In shape, hematopoietic stem cells resemble lymphocytes. (wikipedia.org)
Natural-killer (NK) cells are specialized lymphocytes that have cytotoxic properties in addition to their ability to produce cytokines that assist in the orchestration of adaptive immunity. (medscape.com)
The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)

Bouhassira, Eric E. "Hematopoietic Transplantation: Cancer. (sagepub.com)
Associate Professor Ingrid Winkler is a Senior Research Fellow and head of the Stem Cells and Cancer group at Mater Research, Brisbane, Australia. (edu.au)
New ways to sensitise cancer cells to standard cancer therapy. (edu.au)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
3) We are defining the properties of cancer stem cells that initiate and sustain the unique disease features of acute leukemias through the use of various adoptive animal models. (stanford.edu)

The depletion of NR4A1 significantly enhanced the sensitivity of CRC cells to TSZ-induced necroptosis, while NR4A1 overexpression suppressed these effects, as evidenced by the LDH assay, flow cytometry analysis of cell death, PI staining, and expression analysis of necrosome complexes (RIPK1, RIPK3, and MLKL). (bvsalud.org)

In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)

Primitive and definitive hematopoietic development. (biomedcentral.com)
Distinct anatomical regions of primitive and definitive hematopoietic development in both the mouse and human. (biomedcentral.com)

These data provide a rationale for the administration of anti-LFA-1 antibody after bone marrow transplantation for reducing the graft failure. (elsevierpure.com)

Allogeneic bone marrow transplantation, as we understood it, allows saving lives, but cannot take place without donors. (longdom.org)
If the geno-identical transplantation is impossible, we will direct ourselves towards the national and international register donors of bone marrow. (longdom.org)

Blood stem cells, also known as hematopoietic stem cells (HSCs), are located within the bone marrow and give rise to all mature blood cells in the body throughout a person's life. (cornell.edu)

Innate immunity resides in the skin, mucous membranes, polymorphonuclear (PMN) cells, complement system, and a select group of cells that possess cytotoxic capabilities. (medscape.com)
Natural killer (NK) cells play a crucial role in early immunity after hematopoietic stem cell transplantation because they are the first lymphocyte subset recovering after the allograft. (ashpublications.org)

The endothelial cells help a very small number of stem cells proliferate and reconstitute the entire animal. (cornell.edu)

Hematopoietic stem cells (HSCs) are the stem cells that give rise to other blood cells. (wikipedia.org)
Haematopoiesis is the process by which all mature blood cells are produced. (wikipedia.org)
It must balance enormous production needs (the average person produces more than 500 billion blood cells every day) with the need to regulate the number of each blood cell type in the circulation. (wikipedia.org)
citation needed] Hematopoietic stem cells can be identified or isolated by the use of flow cytometry where the combination of several different cell surface markers (particularly CD34) are used to separate the rare Hematopoietic stem cells from the surrounding blood cells. (wikipedia.org)
Hematopoietic stem cells are essential to haematopoiesis, the formation of the cells within blood. (wikipedia.org)
Hematopoietic stem cells (HSCs) are a rare hematopoietic cell type that can entirely reconstitute the blood and immune systems following transplantation. (stanford.edu)
HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
A human being produces approximately 3 × 10 11 mature blood cells per day, representing 90% of all cells regenerated on a daily basis. (digs-bb.de)
According to this concept, HSCs are activated by an increased demand for mature blood cells in situations such as systemic infection or severe blood loss. (digs-bb.de)
Loss of mature blood cells as well as systemic inflammation does not stimulate additional HSC contribution. (digs-bb.de)
HSCs are characterized by their unique ability to self-renew and give rise to the entirety of the blood and immune system throughout the lifetime of an individual. (longdom.org)
Dr. Jason Butler and his team discovered that aged blood vessels strongly influence the functional characteristics of blood stem cells. (cornell.edu)
This image shows a blood-vessel defect that occurs as a result of aging: the increased presence of reactive oxygen species (magenta) in aged bone marrow-derived blood vessels (depicted in red and green with blue nuclei). (cornell.edu)
The excess buildup of reactive oxygen species can interfere with the ability of blood vessels to support normal stem cell function. (cornell.edu)
We're trying to figure out how we can rejuvenate blood stem cells in order to help people recover faster from these treatment regimens. (cornell.edu)
Additionally, they found a way to harness cells from young mouse blood vessels to rejuvenate old blood stem cells and restore their function. (cornell.edu)
The researchers isolated young and old blood vessel cells from the bone marrow - called bone marrow endothelial cells - and grew them in petri dishes with young or old HSCs. (cornell.edu)
They are like giants, capable of turning into any blood cell in our body. (thenoveldifference.com)
These remarkable cells play a crucial role in the production and maintenance of the body's blood and immune cell supply. (thenoveldifference.com)
These include red blood cells (erythrocytes), white blood cells (leukocytes), and platelets (thrombocytes), all of which are vital for various functions in the body. (thenoveldifference.com)
Hematopoietic stem cells have the unique capacity to continuously self-renew and maintain a pool of stem cells for the lifelong production of blood cells. (thenoveldifference.com)
This regenerative ability ensures a constant supply of blood cells throughout an individual's lifetime. (thenoveldifference.com)
While ER Clark first reported the observation of vascular generation in tadpoles in 1909, and Stockard described blood-vessel development and coined the term angioblast in fish embryos in 1915, it was not until 1920 that F Sabin described the formation of blood cells through the angioblast 'liquefying' within the aorta [ 1 - 3 ]. (biomedcentral.com)
They are particularly active in tissues and organs in which the lifespan of the differentiated cells is short, like blood, skin and the inner cell layer covering the intestinal cavity, as well as in the repair of skeletal muscle after exercise of injury. (pas.va)
Abnormalities in this process lead to a group of diseases known as myeloid malignancies, which include acute myeloid leukaemia-in which the bone marrow produces abnormal white blood cells-and myelodysplastic syndromes, which are caused by too few mature blood cells being produced. (elifesciences.org)
To address this challenge, researchers have been trying to develop reliable, lab-based methods for making the vital, blood-producing component of bone marrow: hematopoietic stem cells (HSCs). (nih.gov)
In the second, researchers employed another approach to convert mature mouse endothelial cells, which line the inside of blood vessels, directly into self-renewing HSCs. (nih.gov)
Those HSCs ultimately find their way into the bone marrow, where they produce a lifetime supply of blood cells. (nih.gov)

What started as a series of microscopic observations in different animal model systems has since evolved into studies of gene expression and regulation, and subsequent protein-protein interactions, cell surface protein expression profiling, and functional mapping of cell fates. (biomedcentral.com)

HSCs are thought to be a reserve population residing in a deeply quiescent ("dormant") state that protects them from metabolic and proliferative stress. (digs-bb.de)

We want to elucidate how hematopoietic perturbations and stressors are resolved on a clonal level by employing genetic barcoding. (digs-bb.de)

These receptors allow NK cells to spare autologous normal cells and to kill cells in which MHC class I expression is down-regulated (eg, by tumor transformation or viral infection) or cells expressing non-self MHC class I alleles unable to engage these receptors (eg, allogenic transplantation settings). (ashpublications.org)

More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
Significant advances have been made in isolating, culturing and reintroducing adult stem cells into tissues. (pas.va)

With this, we analyzed the phenotypes of CD34 + HSCs and showed that primitive HSCs or CD34 + CD33 - cells expressed adhesion molecules such as CD43, CD44, CD11a, CD11c, CD18, and leukocyte adhesion molecule (LAM-1). (elsevierpure.com)
Among CD34 + CD33 - cells, CD18 + cells gave rise to colony-forming cells (CFCs) on stromal layers, but reached a maximum at week 2, after which the number of generated CFCs decreased. (elsevierpure.com)
When CD18 or CD11a antibody was added to a coculture system of CD34 + CD33 - cells with stromal layers, the number of generated CFCs decreased significantly compared with the no antibody control. (elsevierpure.com)

In adults, haematopoiesis occurs in the red bone marrow, in the core of most bones. (wikipedia.org)

The human body is made up of about 220 different kinds of specialized cells such as nerve cells, muscle cells, fat cells and skin cells. (benthamscience.com)
In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research. (stanford.edu)
We also have a long-standing interest in mast cells and the rare human disease mastocytosis, which is characterized by an abnormal accumulation of these cells in various organs. (digs-bb.de)
During human being carcinogenesis or malignancy development, cancerous cells acquire the capability to move important nuclear protein that can impact treatment efficiency. (cancerhugs.com)
Components and Strategies Cell lines and reagents The human being NSCLC cell lines A549, L1650, L1975, L2228, and HCC827 had been attained from American Type Lifestyle Collection (ATCC, Manassas, Veterans administration, USA). (cancerhugs.com)
To test them out, the researchers first used a previously defined protocol to convert human iPSCs into endothelial cells similar to those known to produce HSCs during development. (nih.gov)
They also note that, while their new findings were in animals, they already have some evidence from a previous study to suggest a similar approach might work in human cells [3]. (nih.gov)
Human NK cells also express on their cell surface non-MHC-specific inhibitory receptors, including p75/AIRM1 (siglec-7) that is expressed virtually by all NK cells. (ashpublications.org)

The endothelial cells also protected other organs affected by radiation throughout the body, including the gut and spleen. (cornell.edu)

Hematopoietic stem cells are widely used in medical treatments, particularly in bone marrow transplantation for individuals with hematological disorders like leukemia or aplastic anemia. (thenoveldifference.com)
These inhibitors are selectively inhibitors of nuclear move (SINE), and possess been demonstrated to end up being effective for dealing with specific types of malignancies, including pancreatic tumor, severe myeloid leukemia, mantle cell lymphoma, causing in significant development apoptosis and inhibition of tumour cells with no serious toxicity [20]C[22]. (cancerhugs.com)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)

Advances in fluorescence-activated cell sorter technology have brought about multicolor analysis of cell phenotypes. (elsevierpure.com)

Anatomy34

Organisms16

Diseases54

Chemicals and Drugs37

Analytical, Diagnostic and Therapeutic Techniques and Equipment75

Psychiatry and Psychology2

Phenomena and Processes36

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena2

Information Science4

Named Groups6

Health Care17

Geographicals1

Progenitor cells29

Hematopoiesis10

Progenitors7

Lineages4

Found in the bone ma1

Pluripotent8

Differentiation7

Multipotent3

Peripheral blood2

Role of bone marrow1

HSCT5

Differentiate6

Donor6

Regenerative medicine4

Clinical12

Therapies3

Post-transplantation2

Transplants3

Proliferation2

Allogeneic transplantation1

Embryonic development2

Aorta-gonad-meson1

Mice18

Months after transplantation2

Umbilical3

Reconstitution3

Developmental signalli1

Therapeutic3

Vivo3

Fetal2

Lymphoid2

Chemotherapy3

Tissue12

Vitro4

Immature4

Generate3

Endothelial cell1

ESCs1

Replenish2

Lymphocytes3

Cancer5

Assay1

Malignant1

Definitive2

Graft1

Donors2

Person's1

Immunity2

Proliferate1

Blood28

Functional1

Quiescent1

Clonal1

Allogenic1

Adult stem2

CD343

Occurs1

Human8

Spleen1

Leukemia3

Advances1