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GelsChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, Ion ExchangeChromatography, GasChromatography, Thin LayerChromatography, LiquidChromatography, DEAE-CelluloseMolecular WeightMolecular Sequence DataMass SpectrometryElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelAmino Acid SequenceChromatography, AgaroseKineticsGas Chromatography-Mass SpectrometryChromatography, Reverse-PhaseHydrogen-Ion ConcentrationSpectrophotometry, UltravioletAmino AcidsElectrophoresis, Gel, Pulsed-FieldSubstrate SpecificityIsoelectric FocusingElectrophoresis, Agar GelChromatography, PaperSepharoseDrug StabilitySpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryEscherichia coliChemical FractionationBase SequenceCattlePeptide FragmentsChemistryRabbitsMagnetic Resonance SpectroscopyChemical PhenomenaIndicators and ReagentsTemperatureTrypsinLiverHydrolysisProtein BindingCarbohydratesCalibrationCarbohydrate SequenceReproducibility of ResultsMethodsPeptidesElectrophoresisRecombinant ProteinsCountercurrent DistributionReference StandardsOligosaccharidesChromatography, Micellar Electrokinetic CapillaryAcetonitrilesCloning, MolecularRadioimmunoassayPlant ExtractsBinding SitesBacterial ProteinsStereoisomerismHydroxyapatitesIsoelectric PointMolecular StructureCell LineSolubilitySwineHot TemperatureSpecies SpecificityPolysaccharidesChemical PrecipitationMicrochemistrySensitivity and SpecificityBiological AssayDNACentrifugation, Density GradientCarbohydrate ConformationLimit of DetectionMacromolecular SubstancesIsoenzymesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationProteinsMethanolUltracentrifugationFatty AcidsSequence Homology, Amino AcidOxidation-ReductionSolventsBrain ChemistryTime FactorsAmmonium SulfateGlycoproteinsSpectrometry, Mass, Fast Atom BombardmentImmunodiffusionCells, Cultured
Thin layer chromatoLiquid ChromatographySize-exclusion chroExchange chromatographyReverse Phase HPLCSilica gel columnPurificationStationaryChromatographicPaper ChromatographyPermeationCapillary electrophoresisHydrophilicSeparationsSolventPurityHydrophobicAnalyticalIonsMolecules are separated basedPrinciplePolyacrylamide gel electrophorPolymersArray DetectorMethodsPhaseColumnsLiquidsPolarColumnMolecularFractionsParticlesElectrosprayMixturesAssayAnalysisCompoundsCompositionOligosGlycoproteinsWidelyDetectionMixture are separatedAcidsProteinsSpeciesHighProtein
Thin layer chromato10
Liquid Chromatography17
Size-exclusion chro2
Exchange chromatography6
Reverse Phase HPLC1
  • Oligo purification Gene Link provides polyacrylamide gel electrophoresis purification (PAGE) and Reverse Phase Cartridge (RPC) (as a substitute for Reverse Phase HPLC (RP-HPLC). (genelink.com)
Silica gel column5
  • Separation of the n-hexane fraction using silica gel column chromatography yielded the chloroform:methanol (50:1) fraction, which showed the highest tyrosinase inhibitory activity. (ijpsonline.com)
  • The n -hexane soluble fraction (10 g) was chromatographed on a silica gel column and eluted with CHCl 3 -MeOH (CM, 100:0~1:1) to yield 6 fractions. (ijpsonline.com)
  • 2.Too polar or acidic sample: silica gel column, CN, NH2, HILIC (hydrophilic chromatography) can be adopted. (specartridge.com)
  • 3.If the sample is alkaline: choose a high-purity silica gel column (high-purity silica gel lacks metal impurities and the end-capping of the silica gel) or some modified C18 columns (such as polar embedding technology or alkaline deactivation technology), which will reduce the tailing of alkaline compounds. (specartridge.com)
  • The advantage is that the method is simple to develop, and the disadvantage is fewer supplier and high price or choose HILIC column (silica gel column is used under reversed-phase conditions, a classic method to detect alkaline samples). (specartridge.com)
Purification14
  • Purification services offered include PAGE and various types of HPLC. (idtdna.com)
  • Originally developed for isolating RNA oligos, RNase-free HPLC purification has been extended to DNA oligos for use in applications in which ribonucleases can be problematic. (idtdna.com)
  • RNase-free HPLC purification is performed in an RNase-free environment: reagents, equipment, and lab surfaces are monitored for RNase contamination using RNaseAlertâ„¢ reagent. (idtdna.com)
  • For applications that demand the high oligo purity, we offer dual HPLC purification or dual PAGE and HPLC purification. (idtdna.com)
  • Dual HPLC purification can be used to purify dual-labeled probes and Molecular Beacons bearing NHS ester modifications. (idtdna.com)
  • Reverse osmosis ( RO ) is a water purification system or process that uses to separate ions, unwanted molecules , and larger particles from drinking water by a semi-permeable membrane. (priyamstudycentre.com)
  • Gel purification routinely yields 99% pure full-length product and RPC and RP-HPLC purification yields a purity of 85%-96% depending on the oligo length, base composition and sequence. (genelink.com)
  • Gel purification is the gold standard of oligo purification methods and routinely yields ~99% full length product and is not dependent on size, base composition or sequence. (genelink.com)
  • For oligos over 40mer or shorter oligos to be used in cloning or other precision downstream applications, we recommend gel purification. (genelink.com)
  • Gel purification separates exclusively by charge, which is equivalent to size in linearized DNA. (genelink.com)
  • Last purification by reversed-phase HPLC gave genuine 2, determined by 1H and MS NMR and comparison with literature prices [10]. (lacbiosafety.org)
  • Techniques include protein isolation, purification and characterization, protein assays, immunoassay: Enzyme-Linked Immunosorbent Assay (ELISA), Western Blot, Sodium Dodecyl Sulfate Polyacrylamide (SDS-PAGE), agarose gel electrophoresis, and Polymerase Chain Reaction (PCR) technology. (elac.edu)
  • The Core also provides theoretical and technical expertise for the separation and purification of proteins and peptides and performs "Proteomics" type projects including the identification of proteins separated by 1- and 2-D PAGE gels and identification of protein phosphorylation sites. (nih.gov)
  • Agilent Technologies Model 1100 series HPLC Modular System equipped with refrigerated autosampler, diode array UV detector and refrigerated fraction collector for protein and peptide isolation and purification . (nih.gov)
Stationary17
  • Chromatography is the separation of mixtures into their components as a result of differential distribution of the solutes in order for identification, analyses, purify and/or quantity of the mixture for a mobile medium and for a stationary absorbing medium. (majortests.com)
  • The silica gel is the stationary phase, TLC uses a thin uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic. (majortests.com)
  • The modern definition of chromatography is, it is a physicochemical technique of separation in which the compounds that required to be separated are distributed between two phases, one is called stationary phase (which remains stationary), and the other is a mobile phase (which moves through the stationary phase). (tech-publish.com)
  • Stationary phase, Mobile Phase, and a sample from which components need to be separated. (tech-publish.com)
  • Instead, it retains and reduces the flow of the components within the sample to be tested based on its affinity to the stationary phase, and the compound gets separated at different times. (tech-publish.com)
  • As it continuously flows through the stationary phase, it takes the compounds with it to separate the components of the sample. (tech-publish.com)
  • Normal phase chromatography uses polar particles usually silica as a stationary phase and non polar solvants like Hexane, ethyl acetate, n-butanol and methanol in order of ascending strength. (whatishplc.com)
  • It is another form of chromatography were the stationary phase is also a liquid but held in place using centrifugal force. (whatishplc.com)
  • In this procedure, different analytes in a sample are separated based on Coulombic interactions with a stationary phase. (genelink.com)
  • Normal Phase HPLC uses a polar stationary phase and a non-polar mobile phase, whereas RP-HPLC uses a non-polar stationary phase and a polar mobile phase. (genelink.com)
  • Like other chromatographic processes, HPLC is also a continuous multiple exchange process of solutes between stationary and mobile phases. (hawachhplccolumn.com)
  • Most stationary phases used in reversed phase chromatography are natural hydrophobic substances. (hawachhplccolumn.com)
  • Therefore, the analytes are separated according to their hydrophobic interaction with stationary phases, and the hydrophobic mechanism can also be separated in the same way. (hawachhplccolumn.com)
  • There are also a few substances on the stationary phase, such as the mixed-phase like phenyl hexyl and some other substances, which also exist on these bonded silica gel. (hawachhplccolumn.com)
  • Put it in short, the more similar the separated substance to the stationary phase, the longer the residence time on it. (specartridge.com)
  • This technique separates molecules using the interaction of the protein, glycoprotein or peptide with a hydrocarbon coated stationary phase. (biopharmaspec.com)
  • Chromatography is essentially a separation technique - the sample is carried along by a mobile phase (either gas or liquid) and the components of the sample mixture are separated due to their affinity for the stationary phase. (chromatographytoday.com)
Chromatographic6
  • The Column Chromatography or Liquid Chromatographic systems were a time-consuming method of separation due to the lower solvent flow rate because it was mainly dependent on gravitational force. (tech-publish.com)
  • When the HPLC column is polluted, its chromatographic result is different from that of an unpolluted column. (hawachhplccolumn.com)
  • In this way, different substances in a complex mixed solution can be separated according to time, and the separated substances are sequentially entered into the detector to be detected by the detector, and then the scientific researchers can use these detected signals (chromatographic peaks) to calculate the concentration and content of a substance. (specartridge.com)
  • Liquid chromatographic profiling is the use of chromatographic techniques to separate molecules by different physicochemical properties. (biopharmaspec.com)
  • BioPharmaSpec has extensive experience in employing a comprehensive range of chromatographic techniques to separate molecules by different physicochemical properties. (biopharmaspec.com)
  • There are many different chromatographic techniques - from the relatively simple paper chromatography used in primary schools to separate ink spots - to the complex LC/MS/MS techniques used in advanced research to identify drug metabolites. (chromatographytoday.com)
Paper Chromatography1
Permeation4
  • Gel permeation chromatography (GPC) analysis allows to separate monomers from larger PCO molecules. (wikipedia.org)
  • What is the basic principle for separation in normal phase, reversed phase, ion-exchange and gel permeation chromatography? (majortests.com)
  • The improved drug permeation and higher flux across nasal mucosa with enhancement ratio of 2.75 was observed for flexible liposomal thermoreversible gel F2 compared to normal thermoreversible hydrogel of drug. (ijpsonline.com)
  • To find out about SEC read Characterisation of Polymers Using Gel Permeation Chromatography and Associated Techniques . (chromatographytoday.com)
Capillary electrophoresis1
  • Each Rapid HPLC DNA Oligo is quality controlled by electrospray ionization-mass spectrometry and capillary electrophoresis or analytical HPLC. (idtdna.com)
Hydrophilic4
  • 5) Packing material is hydrophilic methacrylate gel chemically bonded with quaternary ammonium group. (hplc.com)
  • GFC is used to separate water-soluble high polymers such as polysaccharides, proteins, and nucleic acids according to their molecular sizes by using hydrophilic packing materials and aqueous mobile phase. (shimadzu.com)
  • Shim-pack WAX/WCX series columns are chemically-bonded hydrophilic silica gel based ion exchange columns. (shimadzu.com)
  • Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. (shimadzu.com)
Separations2
  • Most of the HPLC separations (over 90%) are RP-HPLC today. (whatishplc.com)
  • This combination enables us to perform automated capillary (0.17 mm columns) RP-HPLC separations of proteolytic digests obtained from minute amounts of proteins (30 ng) in SDS PAGE 1&2D gel slices. (nih.gov)
Solvent6
  • Separation of dried liquid samples with a liquid solvent and a glass plate covered with a thin layer of silica gel. (majortests.com)
  • This separates liquid samples with a liquid solvent and a column composed of solid beads. (majortests.com)
  • His studies mainly focused on separating leaf pigments using a solvent in a column packed with particles. (tech-publish.com)
  • Upon exposure to dry air, N-vinylcarbazole produced an unidentified product that reacted with the methanol extraction solvent to produce a compound that could be separated from other reactants and N-vinylcarbazole by HPLC. (cdc.gov)
  • A mixture of 16:0 MAG, DAG and TAG (in which the MAG and DAG have been converted to the di- and monoacetates, respectively) was readily separated utilizing a system of two Chrompack Chromspher 5 Lipids (TM) columns connected in series, a solvent system of 1.2% acetonitrile in hexane, and flame ionization detection. (usda.gov)
  • ExtractionCIsolation of BengamidesCBengazoles Some from the MeOH supernatant from the sort test was separated by intensifying solvent partition, as well as the CH2Cl2-soluble small fraction was additional purified by silica gel adobe flash Piboserod chromatography to produce a small fraction containing an assortment of bengazoles (2aCg). (lacbiosafety.org)
Purity1
  • Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns. (shimadzu.com)
Hydrophobic2
  • IDT uses HPLC to purify unmodified oligos as well as oligos with complex modifications such as linkers, spacers, modified bases, and hydrophobic modifications. (idtdna.com)
  • Reverse phase chromatography occured in the 70's were chemist were able to install some hydrophobic chains initially C8 and then C18 (a chain of 18 Carbons long) on the silica particles. (whatishplc.com)
Analytical3
  • HPLC is an analytical and as well a preparative technique where a liquid is pumped through a bed of very finely packed particles. (whatishplc.com)
  • Different instruments can be used to purify compounds on a preparatory scale, separate compounds on an analytical scale, or study the ions present in a mixture. (monmouthcollege.edu)
  • Along with spectroscopy, chromatography is one of the major analytical techniques at the disposal of any chemical or biochemical researcher. (chromatographytoday.com)
Ions1
  • In particular, chloride and nitrate ions can be separated well, and the peak for sulfate ion sharpens. (hplc.com)
Molecules are separated based3
  • During SEC, molecules are separated based on size as they pass through a gel column composed of beads with a defined pore size. (biopharmaspec.com)
  • During HIC, molecules are separated based on hydrophobicity/ hydrophilicity . (biopharmaspec.com)
  • As the technique name suggests, molecules are separated based on their size or molecular weight. (chromatographytoday.com)
Principle1
Polyacrylamide gel electrophor2
Polymers2
  • The beginning of HPLC goes back in the 60's where Jim Waters designed a refractive index detector for Dow Chemicals permirring to work on the separation of polymers. (whatishplc.com)
  • And there are also many fillers for reversed-phase chromatography, including polymers, polymer surfaces coated with silica and alumina, inorganic-organic mixtures, coated zirconia, and graphitized carbon. (hawachhplccolumn.com)
Array Detector1
  • Fluorescence in several wavelengths can be detected by an array detector, to detect compounds from HPLC flow. (newworldencyclopedia.org)
Methods3
  • Chlorpyrifos and its oxon can be determined directly using gas chromatography (GC) and selective detection methods (see below). (cdc.gov)
  • MATERIALS AND METHODS: Polyphenol profiles of LE and SE were dereplicated by HPLC-DAD-ESI-MS/MS, aided by standards. (bvsalud.org)
  • To this end, we made use of both culturing and the culture-independent methods of PCR followed by denaturing gradient gel electrophoresis (PCR-DGGE) and pyrosequencing of 16S rRNA gene amplicons. (who.int)
Phase9
  • Normal and Reversed Phase Chromatography What is the relationship between polarity of the mobile phase vs. elution time and resolution in normal phase and reversed phase chromatography? (majortests.com)
  • Reverse-phase (RP) HPLC separates full-length oligo product from truncated products based on relative hydrophobicity. (idtdna.com)
  • This is called reverse phase because it is exactly the reverse of normal phase chromatography. (whatishplc.com)
  • Shim-pack MAqC-ODS I reversed-phase columns are packed with a silica gel containing metal and added octadecylsilyl group. (shimadzu.com)
  • This is similar to the temperature rise of the gas chromatography procedure, except that the GC changes the temperature, while the HPLC changes the mobile phase polarity, allowing the sample components to be separated under optimum conditions. (hawachhplccolumn.com)
  • Reversed phase chromatography is the most widely used technology in HPLC, mainly because it is suitable for the analysis of a large number of non-polar substances and many ionizable and ionic compounds. (hawachhplccolumn.com)
  • Reversed phase chromatography can be used in many applications by using various mobile phases and additives. (hawachhplccolumn.com)
  • 1. The HPLC column is easily plugged by tiny particle impurities, which makes the operating pressure rise and cannot be used, so the mobile phase must be carefully distilled or filtered with a filter membrane. (hawachhplccolumn.com)
  • On the second day, the four compounds being measured are concentrated via online SPE and then chromatographically separated from each other and from other urine biomolecules using reversed phase HPLC. (cdc.gov)
Columns1
  • You also can choose C8-Universal HPLC Column , Phenyl-Ether HPLC Columns , Silica HPLC Column , and other HPLC Columns using. (hawachhplccolumn.com)
Liquids2
  • To separate and analyse complex mixtures or solutions which include liquids and solids of both organic and inorganic origins. (sctimst.ac.in)
  • Every year, approximately one-third of the U.S. population uses DEET-containing insect repellent products with concentrations ranging from 10 to 100% in a variety of liquids, lotions, gels, sprays, sticks and impregnated materials and more than 30 million packages of DEET-containing products are sold annually. (cdc.gov)
Polar2
  • Thus, substrate interactions with the more polar silica backbone of the AG-HPLC column packing (some 30% of the original hydroxy groups of the silica remain unreacted) may contribute to the observed differences in retention. (usda.gov)
  • 1. The sample is polar and weakly acidic: you can choose C18 for detection under the condition of 100% acidic aqueous solution, that is, select a chromatography column that withstands 100% pure water and retains polar compounds well. (specartridge.com)
Column10
Molecular2
Fractions1
  • We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry. (hindawi.com)
Particles1
  • Serum low-density lipoprotein particles (LDL) and high-density lipoprotein particles (HDL) were separated using non-denaturing polyacrylamide gradient gel (3-31%) electrophoresis. (biochemia-medica.com)
Electrospray1
  • Mass Spectrometers: A) ThermoFinnigan ProteomeX HPLC/ELECTROSPRAY IONIZATION MASS SPECTROMETER (HPLC/ESI/MS) system consisting of a Surveyor Capillary HPLC interfaced to a LCQ Deca XP Plus ion trap ESI Mass spectrometer. (nih.gov)
Mixtures1
  • This course provides fundamental concepts and techniques to separate and analyze chemical mixtures, such as biomolecules. (elac.edu)
Assay2
  • Combined with TLC bioautography assay and HPLC comparison, the four antioxidant components separated by silica gel chromatography and further purified by Sephadex LH-20 were determined to be caffeic acid, rosmarinic acid, luteolin and apigenin, respectively. (rsc.org)
  • SEC-HPLC (or UHPLC) is a very robust assay and typically has very good resolution for HMW species, but it is often difficult to resolve LMW species," Moscariello says. (pharmtech.com)
Analysis6
  • Monomers of procyanidins can be characterized by HPLC analysis. (wikipedia.org)
  • Protein and peptide chromatography prior to MS analysis can partly address this issue. (hindawi.com)
  • This is coupled to RP-HPLC to create a second dimension of separation prior to MS analysis. (hindawi.com)
  • Then, HPLC fingerprints of these samples were established and four active components were deduced by multiple correlation analysis. (rsc.org)
  • Paulo is a Graduate Scientist with technical experience in biopharmaceutical analysis, including HPLC and Mass Spectrometry, which he applies to projects from BioPharmaSpec's global client base. (biopharmaspec.com)
  • The separated substances can be used later for further analysis (e.g. (carlroth.com)
Compounds2
  • Using this technique, he had separated different compounds. (tech-publish.com)
  • The ethyl acetate extraction part of the ethanol extraction was separated and six monomer compounds were obtained. (kjcx.ac.cn)
Composition1
  • The type of HPLC selected is dependent on oligo sequence composition and characteristics. (idtdna.com)
Oligos1
  • HPLC-purified oligos offer speed without comprising the quality of your oligos. (idtdna.com)
Glycoproteins1
Widely1
Detection1
  • Examples of when chromatography is used are in pharmaceutical companies in order to find out the amount of chemicals used in each new product, hospitals for detection of sugar/blood levels and law enforcement for comparisons of samples found at a crime scene. (majortests.com)
Mixture are separated1
  • Chromatography is common way in which components of a mixture are separated. (monmouthcollege.edu)
Acids1
  • Three representative flavonoids and two triterpenic acids were quantified by HPLC. (bvsalud.org)
Proteins1
  • Plasma has a significant dynamic range, with more than 10 orders of magnitude separating albumin concentration and the rarest measurable proteins identified to date [ 10 ]. (hindawi.com)
Species1
  • Ion-exchange (IE) HPLC separates full-length oligonucleotides from truncated species based on relative charge difference. (idtdna.com)
High2
Protein1
  • Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)