Hours epithelial cells. Medical search. Frequent questions

Then they infected laboratory mice with the self-destructing Salmonella bacteria and found that replication in the cytosol of mouse intestinal epithelial cells is important for colonization of the GI tract and fuels fecal shedding. (sciencedaily.com)
Redistribution of membrane proteins in isolated mouse intestinal epithelial cells. (rupress.org)
Single mouse intestinal epithelial cells (IEC) may be isolated by the use of a combination of methods used for the isolation of IEC from other species. (rupress.org)

One of the first lines of defense in the GI tract is the physical barrier provided by a single layer of intestinal epithelial cells. (sciencedaily.com)
However, in previous studies, NIAID scientists had observed that some Salmonella replicate rapidly in the cytosol -- the fluid portion -- of intestinal epithelial cells. (sciencedaily.com)
We have begun to establish 3D in vitro human infection models based on Caco-2 and primary intestinal cells mimicking the gastrointestinal barrier. (uni-wuerzburg.de)
Next, we plan to use advanced intestinal tissue models using primary human epithelial cells and apply a constant flow of medium in the apical chamber and pulsatile flow in the vascular compartment within a bioreactor. (uni-wuerzburg.de)
However, clear differences in gene and protein expression towards the small intestine and an, at best, fair prediction accuracy of intestinal drug absorption restrict the usefulness of a model for intestinal epithelial cells. (bvsalud.org)
To overcome these limitations, we evaluated a panel of low-passaged patient-derived colorectal cancer cell lines of the HROC collection concerning similarities to small intestinal epithelial cells and their potential to predict intestinal drug absorption. (bvsalud.org)
All lines expressed multiple transporter molecules, with the expression levels in three lines being close to those of small intestinal epithelial cells. (bvsalud.org)

Furthermore, canonical signaling pathway analysis results showed that the interferon signaling and apoptosis pathways were highly activated in avian H7N9 infected NHBE cells compared with the pdmH1N1 infected cells. (aacc.org)
Both cell lines received an hCG and angiotensin peptides 24-hour treatment, in combina tion or alone followed by cell viability, apoptosis and cell cycle assays performed by flow cytometer (GUAVA). (scirp.org)
After hCG, Ang-(1 - 7), hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments, MCF7 displayed cell viability decrease and mid-apoptosis increase. (scirp.org)
These cells had an increase in late apoptosis and necrosis after AngII Toac, hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments. (scirp.org)
In summary, cell viability was decreased and apoptosis (initial, mid and late) was in creased after hCG and/or Ang-(1 - 7) peptides treatments. (scirp.org)
These results point out hCG and Ang-(1 - 7) as effective compounds to inhibit cell proliferation, since they decrease cell viability and increase apoptosis in both normal and in tumoral breast cells, being the effect more pronounced in the tumoral cell line. (scirp.org)
Tunel assay of cells exposed to 2.4 microg/cm2 MW for 24 hours showed no evidence of apoptosis. (cdc.gov)
Although radiation initiates apoptosis in the hematopoietic and gastrointestinal compartments within the first few hours after exposure, alternative mechanisms of cell death may contribute to injury in these radiosensitive tissues. (bioone.org)
RNAi-mediated attenuation of Tnfrsf1b decreased TNF-α-induced netrin-1 production and augmented epithelial cell apoptosis in culture. (aacrjournals.org)
Extending these observations, colon biopsies from E. faecalis -colonized IL-10 −/− mice exhibited crypt hyperplasia and increased staining for macrophages, TNF-α, netrin-1, NF-κB, Tnfrsf1b, and the proliferation marker proliferating cell nuclear antigen while also displaying a reduction in epithelial cell apoptosis. (aacrjournals.org)
Cell apoptosis was investigated using 4',6-diamidino-2-phenylindole (DAPI), annexin V, and propidium iodide (PI) fluorescence flow cytometry, which was also used to investigate the cell cycle. (medscimonit.com)
Instead, they release diffusable cytokines, which mediate the inflammatory reaction and resultant apoptosis of epithelial cells. (medscape.com)

The colonic epithelial turnover is driven by crypt-base stem cells that express the R-spondin receptor Lgr5. (nature.com)
TNF-α-mediated bystander assays used a murine coculture system of primary colonic epithelial cells and E. faecalis -infected macrophages ( in vitro ), with an interleukin 10 (IL-10)-deficient mouse model of colon cancer that involves long-term colonization with E. faecalis ( in vivo ). (aacrjournals.org)

In this study, we utilized primary normal human bronchial epithelial (NHBE) cells that are cultured at an air-liquid interface (ALI) to mimic the in vivo airway characteristics 30 . (nature.com)
In the new study, researchers used novel lab techniques that allowed them to examine the impact of both diacetyl and 2,3-pentanedione on epithelial cells in a system that closely mimicked the human airway epithelium in vivo. (newswise.com)
The 3dGRO™ Human Lung Organoid Culture System is a serum-free, multi-stage culture system for efficient differentiation of human induced pluripotent stem (iPS) cells to mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. (sigmaaldrich.com)

Normal human bronchial epithelial (NHBE) cell cultures have been an effective model to assess the viral-host interaction. (aacc.org)
To gain global and dynamic gene expression profiles, the NHBE cells cultured from a 24 year old donor were challenged by 3.0 m.o.i. avian H7N9 virus (A/Taiwan/4-CGMH/2014), H1N1pdm virus (A/California/07/2009), or mock control. (aacc.org)
The number of upregulated genes was larger than the numbers of downregulated genes in both groups of virus-infected NHBE cells at both time points. (aacc.org)
After 12 hours infection, 2313(5.40%) transcripts in pdmH1N1 infected NHBE cells, and 7125(15.94%) transcripts in H7N9 infected cells showed significant differences. (aacc.org)
There are significantly different gene expression patterns between pdmH1N1 and H7N9 infected NHBE cells. (aacc.org)
The study results provide valuable viral-host interaction between H7N9 and NHBE cells, which improves our understanding of the pathogenic mechanisms that lead to severe complications. (aacc.org)
Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)
Consistent with this, qRT-PCR confirmed that the expression of multiple genes involved in cilia biogenesis was significantly downregulated by diacetyl and 2,3-pentanedione in NHBE cells. (nature.com)
Using RNA-Seq, we performed global transcriptomic profiling in primary NHBE cells exposed to diacetyl or 2,3-pentanedione. (nature.com)
Our data showed that diacetyl and 2,3-pentanedione induced significant transcriptomic changes, including those related to ciliogenesis, in the primary NHBE cells. (nature.com)
They exposed normal human bronchial epithelial (NHBE) cells to the chemicals for 24 hours. (newswise.com)

The recent findings of the pluripotent epithelial cells demonstrates how quickly the world has changed. (ontheissues.org)
1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
Additionally, lung organoids can be derived from small amounts of patient tissues or pluripotent stem cells to create living biobanks that facilitate personalized biomedical research. (sigmaaldrich.com)
Human pluripotent stem cells are differentiated into definitive endoderm cells using a 4-day induction medium. (sigmaaldrich.com)

Thirty-five hours post infection, any unbound virus was removed, and the researchers added peripheral blood mononuclear cells (PBMC) from health donors. (news-medical.net)
For instance, GLP-1R agonist administration decreased TNFα and IL-6 production by peripheral blood mononuclear cells (PBMC) of obese patients with T2D and diabetic mouse adipose tissue. (justia.com)
To create an infection model closely mimicking the immune reactions occurring in native human tissue we have run a pilot experiment with the above described static tissue models colonized with human peripheral blood mononuclear cells (PBMCs). (uni-wuerzburg.de)

In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
This study aimed to investigate the effects of xanthoxyletin, a plant-derived coumarin, on human oral squamous cancer cells in vitro and in mouse xenografts in vivo . (medscimonit.com)
The most common in vitro model for absorption, distribution, metabolism, and excretion (ADME) purposes is currently the Caco-2 cell line. (bvsalud.org)
Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)
Dive into the research topics of 'Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)

Seam cells are linked to the hypodermis by small adherens junctions along their apical borders and by small gap junctions on their lateral membranes ( SeamFIG 2 ).In a newly hatched animal, there are ten seam cells on each side of the animal (H1-H2, V1-V6 and T). All seam cells, except for H0, divide in a stem-cell-like pattern before each molt. (wormatlas.org)
The apical face of each seam cell faces the cuticle and remains in contact with the cuticle along the full length of the cell (a, b). (wormatlas.org)
We found apical accumulation of both Drosophila E-Cadherin (DE-Cad) and the apical cue Bazooka (Baz) as cells first form. (rupress.org)
These structures maintain apical Baz, accumulate apical Crumbs, and organize polarized cytoskeletons, but display abnormal cell morphology and fail to segregate the basolateral cue Discs large from the apical domain. (rupress.org)
AJs localize to the boundary between the apical and basolateral domains, and are thought to be critical landmarks for establishing epithelial polarity ( Nelson, 2003 ). (rupress.org)
The apical epithelial surface represents the luminal side of the gut and the basolateral side the vascular system. (uni-wuerzburg.de)
Our preliminary results with GFP-positive S . Typhimurium applied to the apical compartment followed by flow cytometry demonstrate robust infection of the epithelial cell layer (20-30 % infected cells after 24 hours at an MOI of 10). (uni-wuerzburg.de)

It is not until the final 3 to 4 hours of shell formation that the bulk of the accumulated pigment is transferred to the protein-rich, viscus fluid secretion known as the cuticle. (ufl.edu)

ICC/IF: HeLa cells. (abcam.com)
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. (novusbio.com)
CBP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat CBP Monoclonal Antibody (Catalog # MAB2676) at 8 µg/mL for 3 hours at room temperature. (novusbio.com)
It can be grown on any of these cell lines treated with cycloheximide: McCoy, HEp-2, HeLa, or buffalo green monkey cells. (medscape.com)

Only the outer serosa layer and the inner columnar luminal epithelial cells were affected by the reperfusion. (nih.gov)

These specialized cells absorb nutrients and are a critical barrier that prevent pathogens from spreading to deeper tissues. (sciencedaily.com)
The goal of the project is to develop new strategies for the synthetic morphogenesis of epithelial tissues. (ibecbarcelona.eu)
Unlike traditional immortalized pulmonary cell lines and primary cells, lung organoids contain various differentiated cell types with complex tissue architecture that more closely resemble in vivo tissues and functionality. (sigmaaldrich.com)
Thus, an improved understanding of the metabolic strategies that enable tissue residency by T cells could inform new approaches to empower immune responses in tissues and solid tumours. (bvsalud.org)

In this study, we aimed to assess host differentially expressed gene signatures in respiratory tract epithelial cells after influenza A virus pdmH1N1 or H7N9 infection. (aacc.org)
Exiting through epithelial cells of the upper respiratory tract, measles virus is transmitted primarily through aerosols. (gla.ac.uk)

Differentiated primary cells represent a variety of cell types such as enterocytes, mucus-producing goblet cells and enteroendocrine cells, which all contribute interactions between host and pathogen. (uni-wuerzburg.de)
in this study, CIC with a separation and distortion of epithelial cells specificity of 94% and sensitivity of 93%, and losses of mucin-secreting goblet cells, was used [14]. (who.int)
CIC the presence and density of goblet cells or is not, however, usually used in children mucin spots and density, size and shape of below 24 months due to the inability of the epithelial cells in relation to specimen area child to cooperate. (who.int)

They are called transitional cells, renal (kidney) tubular cells, and squamous cells. (medlineplus.gov)
If there are squamous epithelial cells in your urine, it may mean your sample was contaminated. (medlineplus.gov)

By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. (scielo.org.ar)
Previously, we have reported neurotrophism exhibited by human amniotic epithelial cells when transplanted after spinal cord injury in bonnet monkeys. (scielo.org.ar)
Amniotic epithelial cells were believed to secrete an 'Epidermal Growth Factor (EGF) - like' factor and exact identification was not made. (scielo.org.ar)
At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial cell conditioned medium not only survived but also showed extensive differentiation. (scielo.org.ar)
It is interesting to note that while factor secreted by amniotic epithelial cells resembles EGF and/or FGF-2 in its biological action, known isoforms of them were not detected. (scielo.org.ar)
Considering the biological closeness between EGF and FGF-2, results indicate the possibility of a novel isoform of these growth factors secreted by amniotic epithelial cells. (scielo.org.ar)
Amniotic epithelial cells were known to express some of neuronal and glial cell markers (Sakuragawa et al. (scielo.org.ar)
Transplantation of amniotic epithelial cells in various regions of central nervous system such as caudate nucleus (Bankiewicz et al. (scielo.org.ar)
Amniotic epithelial cells transplantation in Parkinson model of rats was found to reverse the condition and prevent death in neurons (Kakishita et al. (scielo.org.ar)
2001). In our previous report, we concluded the usefulness of amniotic epithelial cells transplantation in spinal cord injury repair research. (scielo.org.ar)
We had also outlined various biological and social advantages by which, amniotic epithelial cells transplantation excels its precedent viz. (scielo.org.ar)
Recently, our conclusion of usefulness of amniotic epithelial cells transplantation to treat spinal cord injuries has been confirmed in rodent models of spinal cord injury (Zhi-yuan et al. (scielo.org.ar)
Based on the neurotropism exerted by amniotic epithelial cells conditioned medium, a diffusible neurotrophic factor produced by them had been suggested as a possible cause apart from direct cell-to-cell effects (Uchida et al. (scielo.org.ar)
2001) had found amniotic epithelial cells conditioned medium showed neurotrophic effect on rat embryonic day 18 (E18) cortical neurons. (scielo.org.ar)
Amniotic fluid stem cells are now available to open a broad new area of research. (ontheissues.org)

Immortalized human lung epithelial cells, BEAS-2B, were exposed to all MW for 24 hours. (cdc.gov)
However, this preliminary evaluation indicates that human lung epithelial cells exposed to AP-MW and their coated counterparts produces a genotoxic response. (cdc.gov)
Human colorectal adenocarcinoma cell line Caco-2, known to be susceptible to SARS-CoV-2 infection, was used in place of primary lung epithelial cells, as these are known to be difficult to expand and manipulate. (news-medical.net)
Lung organoids are useful three-dimensional (3D) cell culture models for studying human lung development and respiratory diseases including viral infections (SARS-CoV, H1N1, MERS), cystic fibrosis, asthma/COPD, exposure to air pollution and the effects of smoking. (sigmaaldrich.com)
AFE cells may be cryopreserved (SCC301) or further differentiated into branching lung bud organoids using 3dGRO™ Lung Organoid Branching Medium (SCM307) and further matured into branching and alveolar lung organoids using 3dGRO™ Lung Organoid Maturation Medium (SCM308). (sigmaaldrich.com)

Lgr5 is a well-established marker of these basal stem cells in the gastrointestinal tract 1 and lineage tracing experiments have demonstrated that Lgr5 + cells give rise to clonal cell populations establishing entire colonic crypts 2 . (nature.com)
The finding of Wnt-5a as a new diabetic corneal marker regulating wound healing and stem cell function may have implications for other diabetes complications involving impaired wound healing, including diabetic foot ulcers, as they share similar neurovascular, sensory, and immunological compromise with diabetic eye disease, Shah and colleagues say. (medscape.com)

The PCA analysis results showed that after 12 and 36 hours infection, there are significant different transcriptome profiling pattern between pdmH1N1 and H7N9. (aacc.org)
ATP12A, KRT4, and CEACAM5 gene expression decreased 1273-540 fold 36 hours after H7N9 infection. (aacc.org)
Epithelial infection levels differed between three different viruses, but all induced sizable numbers of DEGs, IAV and EBOV ~50% more than SARS-CoV-2. (news-medical.net)
While S . Typhimurium constitutes one of the best studied laboratory models for enteric bacterial infections, S . Typhi interactions with human host cells remain little understood due to the limited availability of primate infection models. (uni-wuerzburg.de)
In agreement with the known restriction of S . Typhimurium infection to the human gut epithelium no dissemination into the subjacent endothelial cell layer could be detected. (uni-wuerzburg.de)
Twenty-four hours post infection with GFP-positive S . Typhimurium, the tissue was disrupted by trypsinization and bystander and GFP+ epithelial cells, endothelial cells, CD14+ monocytes CD56+ NK-cells and CD4+ T-cells were purified. (uni-wuerzburg.de)
The vascular compartment will be supplemented with purified PBMC preparations to understand the role of these cells in human Salmonella infection. (uni-wuerzburg.de)

At each original cell body of the seam syncytium, where the nuclei are positioned, the seam completely interrupts hyp 7 (section through B in inset ), whereas at the ends of the cells, the seam is so thin that hyp 7 runs behind it, enfolding it completely (section through C in inset ). (wormatlas.org)
RESULTS: BrdU-beta-galactosidase double-staining revealed an inverse correlation between the number of BrdU-labeled nuclei and beta-galactosidase-labeled cells as a function of population doubling level (PDL). (rechargebiomedical.com)

Metabolism in the body is a collection of chemical reactions that takes place in the body's cells. (markedbyteachers.com)

Well, in 1999, a very interesting study was performed testing the relationship between retinal cells' telomere length, senescence and population doubling counts. (rechargebiomedical.com)
Beta-galactosidase histochemistry and telomere loss in senescent retinal pigment epithelial cells. (rechargebiomedical.com)
PURPOSE: To investigate the relation of senescence-related beta-galactosidase activity and telomere shortening to replicative senescence in cultured human retinal pigment epithelial (RPE) cells. (rechargebiomedical.com)

Using flow cytometry and immunofluorescence they found that nucleocapsid protein from the virus was expressed in approximately 50% of Caco2 cells. (news-medical.net)
Analyze cells by flow cytometry on day 4. (sigmaaldrich.com)

To address this question, the scientists genetically engineered Salmonella bacteria that self-destruct when exposed to the cytosol of epithelial cells but grow normally in other environments, including the lumen of the intestine. (sciencedaily.com)
The scientists hypothesize that, by hijacking the epithelial cell response, Salmonella amplify their ability to invade neighboring cells and seed the intestine for fecal shedding. (sciencedaily.com)
The use of engineered human tissue models will allow us to study the role of ncRNA in the interplay of human cells and host-adapted Salmonella with unprecedented resolution. (uni-wuerzburg.de)

the basolateral side with primary human endothelial cells [4]. (uni-wuerzburg.de)

This constant replacement of the epithelium is driven by cells located in the base of colonic crypts, which constantly divide and give rise to the various differentiated cell types. (nature.com)

Expression of Lgr5 is controlled by Wnt signaling, which plays a critical role for stem cell turnover in the gastrointestinal tract 2 . (nature.com)
Acute radiation syndrome is caused by depletion of bone marrow cells (hematopoietic syndrome) and irreparable damage to the epithelial cells in the gastrointestinal tract (gastrointestinal syndrome). (bioone.org)

When CB is administered along with the allergen to mice, there is a significantly augmented response in the draining popliteal lymph node including increases in weight, cell numbers, cell proliferation, and local lymph node response duration [ 867 ]. (nanomedicine.com)
Here, we report that the central proinflammatory cytokine TNF-α acts as a diffusible mediator of the bystander effects induced by macrophages, an effect caused by a proliferation of macrophages that trigger epithelial cell production of Netrin-1, a neuronal guidance molecule. (aacrjournals.org)
Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
Xanthoxyletin significantly inhibited the proliferation of oral cancer cells (IC₅₀, 10-30 µM) with lower cytotoxicity for normal cells. (medscimonit.com)

Interestingly, internalization and persistence of planktonic bacteria varied significantly between strains, whereas biofilm bacteria were internalized in similar and higher numbers, and all strains persisted beyond 44 hours, showing a more homogenous phenotype. (lu.se)

Thus, we find that endogenous Rspo3 signaling is a critical determinant of cellular fate within the crypt and stimulates rapid recruitment of differentiated cells for epithelial wound healing and crypt regeneration through induction of Wnt signaling. (nature.com)
Diabetic keratopathy involves impairments in epithelial wound healing, barrier function, and tear production, along with epithelial erosions and keratitis. (medscape.com)
The abnormal wound healing is due to impaired limbal epithelial stem cells, and the new research, published online July 18 in Diabetologia , involved isolation of those cells from 30 donor eyes of humans with and 23 without diabetes. (medscape.com)
However, treatment with various approaches, including exogenous WNT5A methylation inhibitors and a nanoconjugate that inhibits WNT5A suppression, improved corneal epithelial wound healing as well as expression of the limbic epithelial stem cells. (medscape.com)
Overall, Wnt-5a is a new corneal epithelial wound healing stimulator that can be targeted to improve wound healing and stem cells in the diabetic cornea," write Ruchi Shah, PhD, of the Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, and colleagues. (medscape.com)
Epithelial cells from the wound edge migrate across the wound shortly after injury. (merckmanuals.com)
In a surgically repaired wound (healing by primary intention), they form an effective protective barrier to water and bacteria in 12 to 24 hours and resemble normal epidermis within 5 days. (merckmanuals.com)
Generally, the wound is covered within 48 hours by a layer of epithelium, which forms a scab to separate the underlying dermis from the fibrin clot above. (woundsource.com)

These findings suggested the usefulness of them as an alternate source of cells for transplantation approaches to treat neurological disorders (Sakuragawa et al. (scielo.org.ar)

The Integrative Cell and Tissue Dynamics Group is looking for Postdoctoral Researcher to work on engineering organoid systems. (ibecbarcelona.eu)
Generally speaking, this type of healing occurs when there has not been much loss of cells and tissue and the incisions have been closed using stitches, staples, tapes, or adhesives. (woundsource.com)
They said that the earliest site of microscopic localisation of FMDV antigens was the lymphoid follicle-associated epithelium of the pharyngeal mucosa-associated lymphoid tissue of the nasopharynx at six hours after the aerosol inoculation. (thebeefsite.com)
To demonstrate feasibility of transcriptional profiling in multiple major components of the host immune system and the pathogen simultaneously, we purified defined cell populations of the tissue model by cell sorting. (uni-wuerzburg.de)
Compared with the Caco-2 model, three HROC lines demonstrated both higher similarity to jejunal epithelial tissue cells and higher regulatory potential of relevant drug transporters. (bvsalud.org)
Tissue resident memory CD8+ T (TRM) cells offer rapid and long-term protection at sites of reinfection1. (bvsalud.org)
We found that memory CD8+ T cells deployed a range of adaptations to tissue residency, including reliance on non-steroidal products of the mevalonate-cholesterol pathway, such as coenzyme Q, driven by increased activity of the transcription factor SREBP2. (bvsalud.org)

Angiotensin II Activates Extracellular Signal Regulated Kinases via Protein Kinase C and Epidermal Growth Factor Receptor in Breast Cancer Cells," Journal of Cellular Physiology, Vol. 196, No. 2, 2003, pp. 370-377. (scirp.org)

Thus, although epithelial polarity develops in the absence of AJs, AJs play specific roles in maintaining epithelial architecture and segregating basolateral cues. (rupress.org)

Quantification of cell density indicated no significant edema in the myometrium or in the endometrium of uteri exposed to 4 hours cold ischemia and then a normothermic ex vivo reperfusion for 48 hours. (nih.gov)
The researchers established a coculture model using ex vivo blood immunocytes that were in direct contact with virus infected epithelial cells in order to assess immunocyte interactions with SARS-CoV-2 infected cells. (news-medical.net)
Cells cultured under these conditions show more in vivo -like characteristics as judged by morphology, barrier function and protein expression. (uni-wuerzburg.de)
Here, to systematically define the basis for the metabolic reprogramming supporting TRM cell differentiation, survival and function, we leveraged in vivo functional genomics, untargeted metabolomics and transcriptomics of virus-specific memory CD8+ T cell populations. (bvsalud.org)

CD14+ monocytes and B cells often show perturbed transcriptomes in COVID-19 patients, so the analysis was focused on these cells. (news-medical.net)
The change in Caco-2 cells was extremely mild in comparison, showing none of the effects the monocytes suffered from. (news-medical.net)
Profiling of monocytes in cocultures with Caco-2 cells infected with IAV revealed that the SARS-CoV-2 down signature was the most marked at an intermediate range, but the up signature could not be seen. (news-medical.net)

Furthermore, immunofluorescence staining showed that the number of ciliated cells was significantly decreased by the flavoring chemicals. (nature.com)

The adult alae are commonly used as an indicator of hypodermal seam cell terminal differentiation. (wormatlas.org)
India ink is often used as a phagocytosis labeling agent or a cell differentiation tracer because CB is easily administered, is relatively nontoxic, and is easily observed in cells that have taken it up. (nanomedicine.com)
Tight junctional complexes and microvilli structures were detected in all lines, anda higher degree of differentiation was observed in 5/10 cell lines. (bvsalud.org)

All MW produced increased amounts of micronuclei after exposure to 2.4 microg/cm2 for 24 hours, however differences from the effect of coating cannot be detected at this time. (cdc.gov)
In recurrent exposure, the reaction occurs within several hours to 1-2 days, which is consistent with the timing of a secondary cell-mediated immune response. (medscape.com)

Histone adsorption on the surface of carbon particles significantly stimulates their ingestion by rat peritoneal macrophages, hamster kidney fibroblasts, and mouse L-cells [ 861 ], and the adsorption of polycyclic aromatic hydrocarbons (PAHs) and their nonpolar metabolites on the surface of carbon particles ingested by rat alveolar macrophages has been studied [ 862 ]. (nanomedicine.com)

During this process, the cells migrate along the crypt wall towards the surface where they are shed into the lumen. (nature.com)
Epithelial changes begin to occur when basal cells proliferate and migrate toward the incision. (woundsource.com)

We also observed cell viability decrease in MCF10A after Ang-(1 - 7), Ang-(1 - 7) Fmoc and hCG + AngII Toac treatments. (scirp.org)

Voted YES on expanding research to more embryonic stem cell lines. (ontheissues.org)
Voted YES on allowing human embryonic stem cell research. (ontheissues.org)
To provide for human embryonic stem cell research. (ontheissues.org)
Last week, the FDA approved an embryonic stem cell trial for treatment of Macular Degeneration, which is very promising since there is currently no adequate treatment for the condition which is known to affect 2% of those over 50, and 30% of people over 75. (rechargebiomedical.com)

We intend to post all responses which are approved by the Editor, within 14 days (BMJ Journals) or 24 hours (The BMJ), however timeframes cannot be guaranteed. (bmj.com)
Following this the researchers compared monocyte responses to epithelial cells infected with SARS-CoV-2 compared to other viruses, including the respiratory pathogen IAV and EBOV, a hemorrhagic disease associated with strong inflammation. (news-medical.net)
IL-33 activates group 2 innate lymphoid cells (ILC2) to produce the type 2 cytokines IL-5 and IL-13 that initiate innate immunity-driven allergic responses. (justia.com)
In addition, IL-33 polarizes naïve CD4 T cells to differentiate into effector T helper 2 (Th2) cells, which produce IL-4, IL-5, and IL-13 that are responsible for adaptive immunity-mediated allergen-induced responses. (justia.com)
Tumour-infiltrating lymphocytes with characteristics of TRM cells maintain enhanced effector functions, predict responses to immunotherapy and accompany better prognoses2,3. (bvsalud.org)

If the specimen is taken from the patient's genital tract, inform the patient not to urinate 3-4 hours before the specimen is taken. (medscape.com)
Obtain specimen from epithelial cells in the infected site. (medscape.com)
[ 1 ] If the time between specimen collection and inoculation into cell culture is more than 24 hours, freeze the 2SP transport medium and send it to the laboratory with dry ice. (medscape.com)

Here, we use primary nasal epithelial cells (NECs) from children and adults, differentiated at an air-liquid interface to show that the ancestral SARS-CoV-2 replicates to significantly lower titers in the NECs of children compared to those of adults. (plos.org)
The classic timing for a primary cell-mediated immune reaction is 9-14 days after the initiation of the offending drug. (medscape.com)

We identify two populations of cells with active Wnt signaling: highly proliferative Lgr5 + /Axin2 + cells, as well as secretory Lgr5 − /Axin2 + cells. (nature.com)
Cells populations can double only 60 times before replicative senescence (via the Hayflick Limit) dooms their lineage. (rechargebiomedical.com)

Modulation of Helicobacter pylori induced interleukin-8 synthesis in gastric epithelial cells mediated by cag PAI encoded VirD4 homologue. (bmj.com)
This is especially true during the final 3 to 4 hours of shell deposition since it is during this time in the egg-laying cycle that cuticle synthesis and accumulation occur most rapidly. (ufl.edu)

When bacteria invade these cells, the cells are ejected into the gut lumen -- the hollow portion of the intestines. (sciencedaily.com)

This was indicated by major accumulation of extracellular fluid, presence of apoptotic-labeled glandular epithelial layer and vascular endothelium. (nih.gov)

Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)
Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)

Placental stem cells. (scielo.org.ar)
The majority of epithelial cells are therefore short-lived, in contrast to a limited number of long-lived stem cells in the base of the crypts. (nature.com)
While Lgr5 + cells are long-lived and act as stem cells during homeostatic conditions, experimental models have demonstrated that Lgr5 + stem cells per se are not essential for maintaining epithelial integrity 3 . (nature.com)
Since 2 years ago, the last Stem Cell bill, public support has surged for stem cells. (ontheissues.org)

Upon Lgr5 + cell depletion, these cells are recruited to contribute to crypt regeneration. (nature.com)

Specifically, the WNT5A gene was hypermethylated at the promotor region in the diabetic cells and its protein was markedly repressed. (medscape.com)
The M3 protein, but not the M1 or M5 proteins, was required for optimal uptake and persistence of both planktonic and biofilm bacteria inside cells. (lu.se)

The most contagious period of measles is thought to occur four days before the development of a rash, and the aerosol droplets may hang in the air for up to two hours. (gla.ac.uk)

On testing the different mouthwashes on the viability of cells, all four of them at 100% dilution were highly cytotoxic. (news-medical.net)
On further analysis, when epithelial cells were exposed to the mouthwashes for 2 hours, 6.3% ( v/v ) diluted Listerine and 1.5% ( v/v ) diluted chlorhexidine gluconate do not affect the viability of cells. (news-medical.net)
The MTT assay assessed cell viability, and autophagy was detected by electron microscopy. (medscimonit.com)

Some suggest that it is Congress' role to tell researchers what kinds of cells to use. (ontheissues.org)
Intraepithelial FMDV-negative, MHCII/CD11c-double-positive dendritic cells were present in close proximity to FMDV-positive cells, the researchers said. (thebeefsite.com)

2003). Similarly, when transplanted into ischemic cortical areas, they were found to differentiate into 'neuron-like' cells (Okawa et al. (scielo.org.ar)
With time, both enzymes are found distributed over the entire cell surface. (rupress.org)
Significant differences were found in DNA methylation between the cells of those two groups. (medscape.com)
At early time points after aerosol inoculation, viral antigens were found together with cytokeratin-positive pharyngeal epithelial cells. (thebeefsite.com)
High correlation was observed between PD-L1 and IDO1 expressions (Pearson correlation co-efficient = 1) and subsequently high IDO1 expression in tumour cells was found to be significant for PFS (p = 0.006) and OS (p = 0.034). (bvsalud.org)

We demonstrate that Rspo3 is essential for epithelial repair via induction of Wnt signaling in differentiated cells. (nature.com)
We demonstrate that while Rspo3 from myofibroblasts maintains colonic Lgr5 + cells during homeostasis, during injury its main function is not to maintain Lgr5 + cells but to interact with more differentiated cells that express Lgr4 but not Lgr5 and are able to regain expression of Wnt target genes and generate new crypts. (nature.com)

Signals that regulate epithelial regeneration upon stem cell injury are largely unknown. (nature.com)
Chemical injury induced by DSS leads to a loss of both Lgr5 + cells and Axin2 + cells and epithelial regeneration is driven by Axin2 − cells, including differentiated Krt20 + surface enterocytes. (nature.com)
Regeneration requires stromal Rspo3, which is present at increased levels upon injury and reprograms Lgr5 − but Lgr4 + differentiated cells. (nature.com)
This Rspo3-driven regeneration program is supported by injury-induced stromal remodeling, and is essential for epithelial recovery. (nature.com)

It's normal to have a small number of certain types of epithelial cells in urine. (medlineplus.gov)
Your provider may have ordered an epithelial cells in urine test as part of your regular checkup or if the results of your visual or chemical urine tests weren't normal. (medlineplus.gov)
A normal result is typically "few" cells. (medlineplus.gov)
The study included SCC-1 human oral cancer cells and EBTr normal embryonic bovine tracheal epithelial cells, which were treated with 0 µM, 5 µM, 10 µM, and 20 µM of xanthoxyletin for 24 hours. (medscimonit.com)

These have an intricated and complex set of mechanics related to immune response, cell mobility and signaling pathways, and the scientists suggest that the changes cells undergo when exposed to infected cells are profound and far-reaching. (news-medical.net)
To investigate which viral proteins could be involved in the SARS-CoV-2 specific response, Caco-2 cells were transfected with a panel of plasmids encoding for likely candidates. (news-medical.net)
IL-33 is predominantly produced by epithelial cells in response to protease containing aeroallergens and its release is mediated by dual oxidase 1 (DUOX1). (justia.com)

Anatomy74

Organisms15

Diseases8

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes48

Disciplines and Occupations2

Information Science3

Mouse intestinal epithelial cells3

Intestinal7

Apoptosis12

Colonic epithelial2

Airway3

NHBE11

Pluripotent4

Peripheral blood mononuclear cells3

Vitro6

Apical7

Accumulated pigment1

HeLa4

Luminal1

Tissues4

Respiratory tract2

Goblet cells3

Squamous2

Amniotic15

Lung5

Marker2

Infection7

Nuclei2

Metabolism1

Retinal3

Flow cytometry2

Salmonella3

Endothelial1

Various differentiated cell types1

Gastrointestinal tract2

Proliferation4

Interestingly1

Wound8

Transplantation1

Tissue7

Cellular1

Basolateral1

Vivo4

Monocytes3

Immunofluorescence1

Differentiation3

Exposure2

Macrophages1

Migrate2

Observed cell viability1

Embryonic stem4

Responses5

Specimen3

Primary2

Populations2

Synthesis2

Lumen1

Vascular1

Stromal cells2

Stem cells4

Crypt1

Protein2

Occur1

Viability3

Researchers2

Found5

Demonstrate2

Regeneration4

Normal4

Response3