None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase. (jacksonimmuno.com)
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. (antikoerper-online.de)
Antibody3
For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells. (jacksonimmuno.com)
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with peroxidase from horseradish roots. (licor.com)
Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). (cellimagelibrary.org)
Antigen2
An enzyme isolated from horseradish which is able to act as an antigen. (umassmed.edu)
Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. (jacksonimmuno.com)
Enzymes2
In addition to biomedical applications, horseradish peroxidase is one of the enzymes with important environmental applications. (wikipedia.org)
Enzymes are not used up or permanently altered by their environment Peroxidases are found in a range of organisms and function to break down alcohol (H2O2) and creates byproducts of oxygen and water. (ipl.org)
Oxidation of luminol2
Horseradish peroxidase catalyses the oxidation of luminol to 3-aminophthalate via several intermediates. (wikipedia.org)
In vitro , the chemiluminescence-producing oxidation of luminol by the enzyme horseradish peroxidase yields three metabolites, one of which is 3-aminophthalic acid. (nih.gov)
Conjugates5
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. (jacksonimmuno.com)
Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. (jacksonimmuno.com)
20. Histochemical evaluation of mouse and rat kidneys with lectin-horseradish peroxidase conjugates. (nih.gov)
This horseradish peroxidase (HRP) stabilizing buffer is ready-to-use for peroxidase conjugates and as negative control in immunoassays. (immunochemistry.com)
It is to be used as a diluent for peroxidase-conjugates. (immunochemistry.com)
Horse Radish Peroxidase1
Enzymatic Iodination of Protein: Kinetics of Iodine Formation and Protein Iodination Catalyzed by Horse-Radish Peroxidase. (nih.gov)
Substrate1
Structural interactions between horseradish peroxidase C and the substrate benzhydroxamic acid determined by X-ray crystallography. (nih.gov)
Biochemistry1
The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. (wikipedia.org)
Hydrogen peroxide2
Peroxidase, a heme-containing oxidoreductase, is a commercially important enzyme which catalyses the reductive cleavage of hydrogen peroxide by an electron donor. (wikipedia.org)
Horseradish peroxidase is ideal in many respects for these applications because it is smaller, more stable, and less expensive than other popular alternatives such as alkaline phosphatase. (wikipedia.org)
Electron5
Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways. (nih.gov)
Peroxidase was localized, with the electron microscope in the intercellular clefts of the mesothelium, often along their entire lengths, in vesicles adjoining or contiguous with the clefts, and along the peritoneal and basal surfaces of the cell, and also in intracytoplasmic vacuoles. (rupress.org)
Electron-nuclear double resonance of horseradish peroxidase compound I. Detection of the porphyrin pi-cation radical. (northwestern.edu)
Electron-nuclear double resonance spectra of horseradish peroxidase Compound I exhibit resonances from 14N and beta-protons, and most probably alpha-protons as well. (northwestern.edu)
Activation by horseradish peroxidase, which catalyzes one-electron oxidation, produced seven adducts, including a major one (30%) that coeluted with an adduct observed with microsomal (2%) and nuclear (14%) activation. (nih.gov)
ELISA1
Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry due to its monomeric nature and the ease with which it produces coloured products. (wikipedia.org)
Stability1
High concentrations of phosphate severely decrease stability of horseradish peroxidase. (wikipedia.org)
Substrates1
Numerous substrates for horseradish peroxidase have been described and commercialized to exploit the desirable features of HRP. (wikipedia.org)
Vitro2
Peritoneal mesothelium was exposed for 2-60 min to solutions of horseradish peroxidase by incubation in vitro, or after intraperitoneal injection in vivo. (rupress.org)
Benzo[a]pyrene (BP) was bound to DNA by horseradish peroxidase, rat liver microsomes, and rat liver nuclei in vitro and in mouse skin in vivo. (nih.gov)
Bound1
Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). (nih.gov)
Catalytic1
Catalytic polymerization of bisphenol A using a horseradish peroxidase immobilized microporous membrane reactor. (nih.gov)
Catalyze1
The enzyme of turnip peroxidase is added in the equation to catalyze the oxidation. (ipl.org)
MeSH1
Horseradish Peroxidase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
Polymerization1
Herein, the first enzymatic polymerization of horseradish peroxidase (HRP) is reported. (elsevierpure.com)
Signal1
Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. (cdc.gov)
High1
The temperature in our experiment was not very high which didn't result in denaturation of peroxidase. (ipl.org)
Major2
Moreover, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase has become a major tool. (wikipedia.org)
This graph shows the total number of publications written about "Horseradish Peroxidase" by people in this website by year, and whether "Horseradish Peroxidase" was a major or minor topic of these publications. (umassmed.edu)
ORDER1
Bulk Order Inquiry for Peroxidase Stabilizing Buffer ------- (please add any order requirements, including desired quantity, timing, etc. (immunochemistry.com)