Homozygous transgenic mice mice knockout. Medical search. Frequent questions

Loss of the PPM1H phosphatase that is specific for LRRK2-phosphorylated Rab GTPases yields the same cilia loss phenotype seen in mice expressing pathogenic LRRK2 kinase, strongly supporting a connection between Rab GTPase phosphorylation and cilia loss. (elifesciences.org)
Hemizygous transgenic phenotype is expected to be similar to homozygote phenotype. (mmrrc.org)
Heterozygote knockout phenotype is expected to be similar to the wild-type phenotype. (mmrrc.org)
PHENOTYPE: Homozygous mutation of this gene results in increased bone density. (utsouthwestern.edu)
Mutations of follicle stimulating hormone-beta and its receptor in human and mouse: genotype/phenotype. (nih.gov)
BALB/c nude mice: A spontaneous mutation in Foxn1 nu induces abnormal hair growth (nude phenotype). (ncard.org.au)
Mice homozygous for the severe combined immune deficiency spontaneous mutation Prkdcscid, commonly referred to as scid, are characterized by an absence of functional T cells and B cells, lymphopenia, hypogammaglobulinemia, and a normal hematopoietic microenvironmentsevere combined immune deficient phenotype on Non obese diabetic genetic background. (ncard.org.au)
Moreover, hEGFR KI/KI mice display accelerated chondrocyte and osteoblast differentiation, a phenotype that is also present in Egfr -/- mice and has not been previously described. (silverchair.com)

Genetic ablation technology may be able to produce mice with mutations in just about every gene present in their germ line. (wikipedia.org)
Dr. Jackson's primary purpose in creating these mice was to demonstrate experimentally what most prion scientists had already believed for decades: that prion infectivity is encoded in the conformation of misfolded PrP, and therefore can be generated spontaneously from genetic mutations. (cureffi.org)
To our knowledge, there's never been a homozygous human with FFI, but there have been a handful of homozygous E200K CJD cases suggesting that homozygous mutations lead to an earlier age of onset [ Simon 2000 ]. (cureffi.org)
The molecular basis of impaired follicle-stimulating hormone action: evidence from human mutations and mouse models. (nih.gov)

Homozygous knockout mice or mice heterozygoous for a point mutation in exon 7 display an abnormal gait. (jax.org)
An A1903G point mutation in exon 7 results in homozygous lethality. (jax.org)
The invention provides transgenic non-human animals, such as transgenic rodents and transgenic non-human mammalian cells harboring a transgene encoding an APP polypeptide comprising the Swedish mutation. (justia.com)
the invention also provides non-human animals and cells comprising a transgene encoding an APP comprising the Swedish mutation and further comprising functionally disrupted endogenous APP gene loci, transgenes and targeting constructs used to produce such transgenic cells and animals, transgenes encoding human Swedish mutation APP polypeptide sequences, and methods for using the transgenic animals in pharmaceutical screening and as commercial research animals for modeling neurodegenerative diseases (e.g. (justia.com)
2018). Here, we show that cilia loss is seen as early as 10 weeks of age in these mice and also in two other mouse strains carrying the most common human G2019S LRRK2 mutation. (elifesciences.org)
homozygous for the Psen1 mutation and homozygous for the co-injected APPSwe and tauP301L transgenes (Tg(APPSwe,tauP301L)1Lfa)) are viable, fertile and display no initial gross physical or behavioral abnormalities. (mmrrc.org)
Lindquist's lab had studied scrapie and CJD for years, so to demonstrate conclusively that prion infectivity could arise spontaneously from a genetic mutation, Jackson needed to be able to rule out the possibility that any mice in the experiment had simply acquired prion disease from prions left in the mouse cages from other experiments. (cureffi.org)
ki stands for knock-in: these mice carry the 3F4 epitope and FFI mutation as engineered changes to what is otherwise mouse PrP. (cureffi.org)
He also created control ki-3F4-WT mice as a control: mice with the 3F4 knock-in but no FFI mutation. (cureffi.org)
You inject the mutated cells into a blastocyst and as it develops, parts of the mouse carry the mutation and parts don't. (cureffi.org)
If the mutation winds up in the germline , you can breed the mouse and get offspring which carry the mutation in every cell in their bodies. (cureffi.org)
Then you have to start crossing these mice to the genetic background that you want the mutation on, at a cost of about 9 or 10 weeks per generation at best. (cureffi.org)
in adult acinar cells harboring oncogenic mutation accelerated acinar to ductal reprogramming resulting in mucinous PDAC precursor lesions in mice. (morainetownshipdems.org)
Mice homozygous for the Rag1 tm1Mom mutation produce no mature T cells or B cells. (ncard.org.au)
Our data indicate, for the first time, a requirement for MAPK signaling in the developing XY gonad in order to facilitate normal expression of SRY and the downstream testis-determining genes and also suggest that reduced dosage of MAP3K4 may be the cause of a previously described autosomal sex-reversing mutation in the mouse. (plos.org)

There are over 400 documented inbred strains of mice. (uwm.edu)

Transgenic mice carrying an Nrl-EGFP allele and homozygous for either knock-out of rhodopsin (Nrl-EGFP, Rho-/-) or heterozygous for knock-in of P23H mutant rhodopsin (Nrl-EGFP, RhoP23H/+) were used in this study. (ox.ac.uk)
Cyagen Knockout Catalog Models 라이브러리는 전 세계 연구자들에게 3개월 이내 빠르게 전송되는 바로 사용할 수 있는 16,000마리 이상의 하이브리드 녹아웃(KO) 마우스(heterozygous gene knockout mice)를 제공했습니다. (cyagen.com)
표준 하이브리드 모델(heterozygous model) 결과물(deliverables) 외에도 동형 접합 번식( homozygous breeding) 및 표현형 분석과 같은 다양한 동물 모델 지원 서비스를 제공할 수 있습니다. (cyagen.com)
In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
Two-month-old wild-type ( BACE1 +/+), heterozygous ( BACE1 +/-) and homozygous BACE1 knock-out ( BACE1 -/-) mice were injected with 5-bromo-2'-deoxyuridine (BrdU), a thymidine analog that is incorporated into replicating cells. (eneuro.org)

Mice homozygous for a knock-out allele exhibit abnormalities in retinal rod cell outer segment morphology and rod electrophysiology. (jax.org)
Cell populations derived from these transgenic mice can be distinguished from syngeneic host and other mice with the alternate allele via flow cytometry. (ncard.org.au)

The development of tools to manipulate the mouse genome, including knockout and transgenic technology, has revolutionized our ability to explore gene function in mammals. (elifesciences.org)

To explore VHL as a regulator defining nephron progenitor self-renewal versus differentiation, we bred Six2-TGC tg mice with VHL lox/lox mice to generate mice with a conditional deletion of VHL from Six2 + nephron progenitors. (lww.com)
To determine its biological function in reproduction, we used Cre (cAMP response element)/LoxP technology to generate GnRH neuron-specific Jak2 conditional knock-out (Jak2 G −/− ) mice. (jneurosci.org)
Conditional knockout of Gli3 causes a significant larger brain size, which can be recovered by knockdown of miR-7. (frontiersin.org)
We show that the conditional depletion of the m 6 A reader protein Ythdf2 in mice causes lethality at late embryonic developmental stages, with embryos characterized by compromised neural development. (biomedcentral.com)
By employing a conditional knock-in approach using the human EGFR cDNA mice humanised for EGFR ( hEGFR KI/KI )were generated. (silverchair.com)
We and others have synthesized light-activatable cMOs that allow spatiotemporal control of RNA splicing or translation, complementing the use of conditional knockouts to study stage- and tissue-specific differences in gene function. (cdc.gov)

To elucidate differences in the functions of these two proteins, comprehensive survival studies with Xpa −/− , Xpc −/− and wild-type control female mice in a pure C57BL/6J background were done. (aacrjournals.org)
On the C57BL/6J background, embryos homozygous for byg exhibit consistent XY gonadal sex reversal. (plos.org)
The model was created by targeting the Ccr5 gene in E14TG2a embryonic stem cells derived from 129P2/Ola mice and injecting the targeted cells into C57BL/6 blastocysts. (taconic.com)
response to influenza infection has been well characterized in C57BL/6 (B6,H2b) mice. (cdc.gov)

This causes the genes to become homozygous (same strain) at nearly all the loci on the chromosomes. (uwm.edu)
Expression of a number of neuroinflammatory and synaptic plasticity genes was reduced by HFD-C diet in control mice, and less affected by HFD-C diet in Stat4ΔLysMLdlr-/- mice. (bvsalud.org)
The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)
Transgene expression using viral vectors provides an alternative, which allows for temporal expression of genes in mice with reduced cost and effort. (nature.com)

and 3) characterization of functional domains in different sperm ligands (fertilin-α and fertilin-β in the mouse and lysin in the abalone) that recognize cognate binding sites on the egg surface. (bioone.org)

Established in response to a need for in-house mouse services, the LUTCF provides expertise in cryopreservation of embryos by IVF or natural matings, sperm cryopreservation, rederivation services, strain expansion by IVF, ES morula/blastocyst injections, pronuclear DNA microinjections, and injection of CRISPR edited DNA. (lu.se)
This is currently the most popular method for the generation of targeted knock-out and knock-in models by ES-cell injections into 8-cell morulae and/or blastocysts of mouse embryos. (lu.se)

By embryonic day 15.5, kidneys of nephron progenitor cell-specific VHL knockout mice begin to exhibit reduced maturation of nephron progenitors. (lww.com)
VHL knockout nephron progenitors also exhibit persistent Six2 and Wt1 expression, as well as decreased mitochondrial respiration and prolonged reliance on glycolysis. (lww.com)
Homozygous knockout mice for this gene exhibit increased bone density and volume, while overexpression of this gene in a transgenic mouse causes bone defects resulting in spontaneous rib fractures. (utsouthwestern.edu)
BACE1 -/- mice exhibit significantly more BrdU+ cells compared to BACE1 +/+ or BACE1 +/- mice. (eneuro.org)

Floxed EGFR mice were bred with homozygous LysM-Cre (LMC) transgenic mice to yield myeloid-specific EGFR knockout (mKO) mice. (bvsalud.org)
Mice lacking the epidermal growth factor receptor (EGFR) develop epithelial defects and a neurodegenerative disease and die within the first month of birth. (silverchair.com)
However, these mice are growth retarded and show skin and hair defects similar to Egfr -/- mutants. (silverchair.com)

From these mice, a homozygous colony can be created through breeding. (wikipedia.org)
For example, the pancreatic cancers cell series MIA PaCa-2 includes a homozygous deletion of and ectopic appearance of ARID1B significantly inhibited colony development and anchorage unbiased growth from the Rabbit Polyclonal to MAEA cells [84]. (morainetownshipdems.org)
The colony was maintained by incrossing homozygous mice. (taconic.com)

Likewise, deletion in mice PDAC tumors (mutant and hemizygous deletion in mice with pancreatic appearance of turned on KRAS led to IPMN that advanced to PDAC [49,82]. (morainetownshipdems.org)

This is accomplished through the administration of one or more transgenes into a fertilized mouse oocyte's pronucleus. (wikipedia.org)

To study the potential role in this metabolic shift of von Hippel-Lindau (VHL), a protein component of a ubiquitin ligase complex, the authors generated nephron progenitor cell-specific VHL knockout mice. (lww.com)
Knockdown of microRNA (miRNA) miR-7 can rescue abnormal brain morphology in Gli3 knockout mice by recovering progenitor specification, neuronal production and migration through a counter-balance of the Gli3 activity. (frontiersin.org)

16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

One naturally occurring genetic defect in mice results in an animal with no hair that is called a nude. (uwm.edu)
homozygous nude mice lack T cells and suffer from a lack of cell-mediated immunity. (ncard.org.au)

This manuscript reports data consistent with a new and unanticipated phenomenon: that Cre or its mRNA may be transmitted between tissues in the mouse and that the male reproductive tract (epididymis) appears to be the most common target of such transported molecules. (elifesciences.org)
The histology of the reproduction members, seminiferous tubules and the epididymis of transgenic mice were observed with coloring. (separase.com)

Nuclear positioning, germinal vesicle breakdown, spindle migration, spindle rotation, chromosome segregation, and polar body extrusion are the most critical cellular processes during oocyte meiosis I and II, and a growing number of studies primarily using the mouse oocyte model revealed that actin filaments were critical for these processes, especially for spindle migration. (bioone.org)

When you mutate embryonic stem cells to create a mouse model, you don't get a purely mutant animal right away. (cureffi.org)

Plastic restraint devices can be used to hold mice and other rodents for longer periods of time. (uwm.edu)

동형 접합적 돌연변이 마우스(homozygous mutant mice )는 손상, 불임, 치명적 등 다양한 영향을 미칠 수 있기 때문입니다. (cyagen.com)
Previously, we showed that cholinergic interneurons of the dorsal striatum lose their cilia in R1441C LRRK2 mutant mice (Dhekne et al. (elifesciences.org)
our data support a model in which LRRK2 and PPM1H mutant mice show altered responses to critical Hedgehog signals in the nigrostriatal pathway. (elifesciences.org)

Superior specificity is achieved by combining Cre-mediated AAVR overexpression with whole-body knockout of endogenous Aavr , which is demonstrated in heart cardiomyocytes, liver hepatocytes and cholinergic neurons. (nature.com)

We used histologic, immunofluorescence, RNA sequencing, and metabolic assays to characterize kidneys from these mice and controls during development and up to postnatal day 21. (lww.com)
Compared with controls, VHL knockout kidneys are smaller and developmentally delayed by postnatal day 1, and have about half the number of glomeruli at postnatal day 21. (lww.com)
The present study showed that Polo-like kinase 1 (Plk1) was upregulated in the kidneys of CKD patients and mice subjected to unilateral ureteral obstruction (UUO) with location in proximal tubules and tubulointerstitial fibroblasts. (nature.com)
In CKD patients or obstructive mice model, we observed that Plk1, a critical mitotic regulator, was upregulated in kidneys. (nature.com)

We demonstrate that transgenic AAVR overexpression greatly increases the efficiency of transduction of many diverse cell types, including muscle stem cells, which are normally refractory to AAV transduction. (nature.com)
The system allows for the generation of mice with specific overexpression of AAVR through breeding with available Cre mouse lines, as well as the option to detarget AAV from other tissue when paired with Aavr -KO mice. (nature.com)
Sepase is oncogene and overexpression is sufficient to induce mammary tumors in mice. (separase.com)
Acute or chronic overexpression of sepaase in the mute glands leads to aneuploidy and tumorigenesis and the inhibition of the enzymatic separation activity decreases the growth of the xenograms of the human chest tumor in the mouse. (separase.com)

The mice with Knockout Ricor also showed an aberrant location of the BTB associated proteins, which were dispersed over the entire seminiferous epithelium, but the expression levels of the protein remained stable. (separase.com)

deletion in mice induced irritation, development of PanINs, and mucinous cysts [49]. (morainetownshipdems.org)

Preovulatory LH surge levels were blunted in Jak2 G −/− mice, which was correlated with reduced GnRH neuronal activation as assessed by c-Fos. (jneurosci.org)
That need motivated two decisions about the mouse model he generated: first, he chose FFI as the disease to study because FFI (in humans) was known to have phenotypes distinct from CJD and other prion diseases: insomnia, hyper- or hypothermia, astrogliosis and neuronal loss localized in the thalamus. (cureffi.org)
Compared to BACE1 +/+ mice, fewer BrdU+ cells colocalized with the neuronal marker, NeuN, in the dentate gyrus of BACE1 -/- mice. (eneuro.org)

Here, we show that GABAergic neurotransmission and spontaneous network activity is perturbed in the hippocampus of neonatal mice lacking GluK1 KARs selectively in GABAergic neurons. (biomedcentral.com)

Breeding is necessary not only to perpetuate your mouse line but even just to get a knock-in (or transgenic) mutant in the first place. (cureffi.org)

Electroporation of primary T cells with Alt-R RNP complex targeted to alpha chain of the T-cell receptor routinely results in over 80% knockout of surface TCR complex, without detectable toxicity ( Figure ). (genengnews.com)
BALB/c based mouse model in which the administration of diphtheria toxin (DT) leads to specific depletion of regulatory T cells (Treg) due to expression of DT receptor-enhanced GFP under the control of the Foxp3 promoter. (ncard.org.au)

GnRH mRNA levels were reduced in Jak2 G −/− mice when compared with controls, while the number of GnRH neurons was equivalent, indicating a reduction in GnRH gene expression. (jneurosci.org)
These results demonstrate an essential role for Jak2 signaling in GnRH neurons for normal reproductive development and fertility in female mice. (jneurosci.org)
In adult male mice, the absence of GluK1 in GABAergic neurons led to stronger hippocampal gamma oscillations and enhanced theta-gamma cross frequency coupling, coinciding with faster spatial relearning in the Barnes maze. (biomedcentral.com)
examined the proliferation, survival, and differentiation of newborn neurons in the dentate gyrus of mice deficient in BACE1 . (eneuro.org)
The reduced number of differentiated neurons in the dentate gyrus of BACE1 -/- mice was not countered by increased differentiation of NPCs into astrocytes or oligodendrocytes. (eneuro.org)
BACE1 -/- mice displayed increased NPC proliferation, but overall, these cells do not mature into neurons that could potentially compensate for the learning and memory defects associated with AD. (eneuro.org)

In studies of in vitro hippocampal slices from control Stat4fl/flLdlr-/- mice fed a HFD-C diabetogenic diet, we show that Schaffer collateral-CA1 synapses exhibited larger reductions in activity-dependent, long-term potentiation (LTP) of synaptic transmission, compared to mice fed a standard diet. (bvsalud.org)

More laboratory mice are used in research every year than any other animal species. (uwm.edu)
Second, he engineered an additional change to the mice's Prnp DNA sequence to introduce a species barrier between his mice and previous mice studied in the lab. (cureffi.org)

Synaptic transmission and long-term potentiation are demonstrably impaired in mice 6 months of age. (mmrrc.org)

Female Jak2 G −/− mice exhibited significantly delayed puberty and first estrus, abnormal estrous cyclicity, and impaired fertility. (jneurosci.org)

CD4 TCR transgenic mouse whose CD4 T cells predominantly recognise the MHC class II (I‑A d )‑restricted TCR specific for the PR8 influenza HA peptide 126-138 (HNTngvtaacshe) ( PMID: 7889402 ). (ncard.org.au)

Cyagen 의 번식 서비스는 유전자 공학 mouse/rat 모델( genetically engineered mouse/rat models) 을 설계하고 맞춤하는 것 외에도 신뢰할 수 있고 경제적이며 효율적인 mouse/rat 번식군을 제공하여 당신의 연구 요구를 지원합니다. (cyagen.com)
Inbred lab mice are designed to be as genetically identical as possible so that experiments are more tightly controlled. (cureffi.org)
Influenza-specific CD8+ T cells recognize multiple of reagents and genetically modified mouse models has viral epitopes on target cells and antigen-presenting cells. (cdc.gov)

Dynamic in vivo quantification of rod photoreceptor degeneration using fluorescent reporter mouse models of retinitis pigmentosa. (ox.ac.uk)
Here we describe a novel technique for the in vivo visualisation of rod photoreceptors which permits semiquantitative assessment of outer retinal degeneration, and validate this approach in two mouse models of retinitis pigmentosa (RP). (ox.ac.uk)
Additionally, Xpc -deficient mice exhibited a strong increase in mutant frequency in lung compared with Xpa −/− mice, whereas in both models mutant frequency is increased in liver. (aacrjournals.org)
Mouse models of influenza A virus pneumonia provide to heterologous viral strains. (cdc.gov)

The frustrating thing about being a postdoc doing mouse experiments-or a rare disease advocate hoping for progress in drug testing-is that it takes years to get any results. (cureffi.org)
To speed up results from the mouse model, Dr. Jackson bred the mice to be homozygous for the knock-in disease gene. (cureffi.org)
These results indicate that complete, but not partial loss, of BACE1 activity increases cell proliferation in the dentate gyrus of young adult mice. (eneuro.org)

Asbestos induced mesothelioma mouse model in which expression of the Simian Virus 40 large T antigen (SV40 TAg) is controlled by mesothelin tissue‑specific promoter. (ncard.org.au)

CD8 TCR transgenic mouse whose CD8 T cells predominantly recognise the MHC class I (H-2K d ) restricted CL4 epitope of PR8 influenza HA protein. (ncard.org.au)
The testicular sertoli cells are essential for maintaining the integrity and function of BTB and ensuring normal spermatogenesis in the mouse. (separase.com)
The number of BrdU-positive (BrdU+) cells was significantly higher in the dentate gyrus of BACE1 -/- mice compared to both BACE1 +/- and BACE1 +/+ mice ( Fig. 1 ). (eneuro.org)
Emerging evidence indicates that findings from from PR8 that are processed to generate peptides recog- mouse studies are pertinent to immunopathology in human nized by influenza-specific CD8+ T cells. (cdc.gov)

Xpc −/− mice displayed a significant increase in lung tumors and a trend toward increased liver tumors compared with Xpa -deficient or wild-type mice. (aacrjournals.org)
Xpa −/− mice showed a significant elevation in liver tumors. (aacrjournals.org)

Interestingly, the neurodegeneration is fully rescued in hEGFR KI/KI mice, however, they develop a severe heart hypertrophy with semilunar valve abnormalities. (silverchair.com)

Although inactivation or 'knockout' of VMAT2 (brain isoform of VMATs) in mice held promise as an animal model to test the hypothesis, the complete inactivation (homozygous) in mice is lethal shortly after birth, preventing their use in the study of aging related neuropathogenesis. (michaeljfox.org)
The goal of this project is to generate an animal model that conditionally inactivates VMAT2 in adult or aging mice. (michaeljfox.org)
Specifically, we will establish a transgenic animal model in that we can achieve a spatial and temporal inactivation of VMAT2 gene in the brain. (michaeljfox.org)
The enhanced efficacy and exquisite specificity of SELECTIV has broad utility in development of new mouse model systems and expands the use of AAV for gene delivery in vivo. (nature.com)
2022년 12월 31일까지 Knockout Catalog Model 라이브러리에서 주문하여 혜택을 잠급니다. (cyagen.com)
As Prion Alliance has recently decided to make testing candidate therapeutic molecules in these mice one of its research priorities , I've spent a lot of time educating myself on this mouse model and am going to document the key points in this post. (cureffi.org)

Pharmacological inhibition, genetic silencing or knockout of Plk1 attenuated obstructive nephropathy due to suppressed fibroblast activation mediated by reduced autophagic flux. (nature.com)

These data demonstrate that MAP3K4-dependent signalling events are required for normal expression of Sry during testis development, and create a novel entry point into the molecular and cellular mechanisms underlying sex determination in mice and disorders of sexual development in humans. (plos.org)

Temporal control of gene expression and ablation can be attributed to evolving transgenic and gene-therapy technologies. (wikipedia.org)
Importance of the filamin A-Sav1 interaction in organ size control: evidence from transgenic mice. (nih.gov)
Stat4ΔLysMLdlr-/- mice, which lack Stat4 under control of the LysMCre promoter, were resistant to HFD-C induced impairments in LTP. (bvsalud.org)
In contrast, Schaffer collateral-CA1 synapses in Stat4ΔLysMLdlr-/- mice fed the HFD-C diet showed larger LTP than control Stat4fl/flLdlr-/- mice. (bvsalud.org)

and report here a role for the Jak2 signaling molecule in the regulation of reproductive development and function in female mice. (jneurosci.org)
Brigid Hogan is a developmental biologist who has worked extensively on the early stages of mouse development and is now unravelling the mysteries of lung organogenesis. (biologists.com)
We identified a mouse mutant that causes embryonic gonadal sex reversal: the development of ovaries in an XY embryo. (plos.org)

Immunodeficient mouse strains are invaluable when assessing the role of immune cell subsets in anti‑tumour immunity. (ncard.org.au)
Methods AMH CRE positive mice are homozygous for RICTOR LOXP with a specific RICTOR sertoli cell removal have been obtained by AMH CRE cross-high mice with RICTOR LOXP mice. (separase.com)
Cell survival was similar in BACE1 +/+, BACE1 +/-, and BACE1 -/- mice that received a single BrdU injection at two months of age and were killed four weeks later. (eneuro.org)
In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)

Skeptics wouldn't be able to argue that mice with these phenotypes had simply acquired prion disease from other mice or laboratory contaminants. (cureffi.org)
The resulting blockade of RNA splicing or translation allows loss-of-function phenotypes to be determined within days, contrasting the months that can be required to obtain homozygous mutants. (cdc.gov)

Common laboratory inbred mouse strains such as BALB/c and C57Bl6/J form the backbone of much of our pre-clinical work. (ncard.org.au)
The Ccr5 Knockout mouse was developed by William A. Kuziel in the laboratory of Nobuya Maeda at the University of North Carolina. (taconic.com)

The mGV of superior retina was significantly greater than that of inferior retina in RhoP23H/+ (P = 0.0024) but not in age-matched Rho+/+ (P = 0.45) or Rho-/- (P = 0.65) mice reflecting histological findings. (ox.ac.uk)
Secretion of GnRH is also reduced as basal serum luteinizing hormone (LH) levels were significantly lower in female Jak2 G −/− mice while the pituitary responded normally to exogenous GnRH. (jneurosci.org)

Immunohistochemical observation of NOS expression In H&E stained tissues, centrally nucleated fibers, which represent muscle regeneration, were observed in only mdx mice (Figs. (Figs.7a-e).7a-e). (cxcrinhibitor.com)
Figure 7 nNOS expression and localization in vascular endothelium and cremaster muscles of mice. (cxcrinhibitor.com)

MPS1 Kinase dependent on the location of median kinase-dependent centromere Protection of coheche in mice meiosis of mice I. (separase.com)
Here we report a role for the phylogenetically ancient mitogen-activated protein kinase (MAPK) signalling pathway in mouse sex determination. (plos.org)

The immunohistochemical analysis showed that nNOS was observed mainly Inhibitors,research,lifescience,medical at the sarcolemma rather than in the endothelium and vascular smooth muscle in B10 and eNOS-/- mice (Figs. (Figs.7b7b and and7h).7h). (cxcrinhibitor.com)
In particular, mdx and nNOS-/- mice showed impaired vasodilation in parallel occlusion, Inhibitors,research,lifescience,medical whereas responses to ACh and SNP were unaltered. (cxcrinhibitor.com)

This mutant mouse exhibits plaque and tangle pathology associated with synaptic dysfunction, traits similar to those observed in Alzheimer's disease patients. (mmrrc.org)

The data serve as a timely warning to mouse researchers about an unexpected complication of Cre-mediated gene manipulation. (elifesciences.org)
The Lund University Transgenic Core Facility (LUTCF) is a core resource whose primary goal is to deliver most up-to-date methodologies in transgenic technology to both our researchers and external users. (lu.se)

Organisms6

Diseases1

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes5

Phenotype8

Mutations4

Mutation15

Inbred strains1

Heterozygous5

Allele2

Genome1

Conditional6

C57BL4

Genes4

Ligands1

Embryos2

Exhibit4

EGFR3

Colony3

Hemizygous1

Transgenes1

Progenitor2

Zebrafish1

Nude2

Epididymis2

Chromosome1

Embryonic stem1

Rodents1

Mutant mice3

Endogenous1

Kidneys4

Overexpression4

Proteins1

Deletion1

Neuronal3

Hippocampus1

Breeding1

Receptor2

Neurons6

Hippocampal1

Species2

Long-term potenti1

Abnormal1

PMID1

Genetically3

Models4

Results3

Tissue-specific1

Cells4

Tumors2

Abnormalities1

Model6

Fibroblast1

Demonstrate1

Control4

Development3

Cell4

Phenotypes2

Laboratory2

Significantly2

Expression2

Kinase2

Inhibitors2

Pathology1

Researchers2