• RNase P is unique from other RNases in that it is a ribozyme - a ribonucleic acid that acts as a catalyst in the same way that a protein-based enzyme would. (wikipedia.org)
  • Bacterial RNase P has two components: an RNA chain, called M1 RNA, and a polypeptide chain, or protein, called C5 protein. (wikipedia.org)
  • This crystal structure confirms earlier models of substrate recognition and catalysis, identifies the location of the active site, and shows how the protein component increases RNase P functionality. (wikipedia.org)
  • RNA from bacterial RNase Ps retains its catalytic activity in the absence of the protein subunit, i.e. it is a ribozyme. (wikipedia.org)
  • Although the archaeal and eukaryotic holoenzymes have a much greater protein content than the eubacterial ones, the RNA cores from all the three lineages are homologous-helices corresponding to P1, P2, P3, P4, and P10/11 are common to all cellular RNase P RNAs. (wikipedia.org)
  • In archaea, RNase P ribonucleoproteins consist of 4-5 protein subunits that are associated with RNA. (wikipedia.org)
  • The structures of protein subunits of archaeal RNase P have been resolved by x-ray crystallography and NMR, thus revealing new protein domains and folding fundamental for function. (wikipedia.org)
  • Five of these protein subunits exhibit homology to archaeal counterparts. (wikipedia.org)
  • These protein subunits of RNase P are shared with RNase MRP, a catalytic ribonucleoprotein involved in processing of ribosomal RNA in the nucleolus. (wikipedia.org)
  • Thus, human RNase P differs in the details of its protein composition, and perhaps in the functions of some of these proteins, from the yeast enzyme. (elsevierpure.com)
  • Dive into the research topics of 'Rpp14 and Rpp29, two protein subunits of human ribonuclease P'. Together they form a unique fingerprint. (elsevierpure.com)
  • RNase protection assays and chemokine protein production analysis validated the microarray results, as cross-linking the Ly-49D mouse NK receptor induced high levels of IFN-γ, lymphotactin, macrophage-inflammatory protein (MIP)1α, and MIP1β. (aai.org)
  • These Ly-49 inhibitory receptors, as well as inhibitory KIRs, contain cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIMs) that are phosphorylated upon stimulation, leading to the recruitment of Src homology 2 domain-containing protein tyrosine phosphatase (SHP-1) and attenuation of intracellular signals ( 1 , 4 , 5 ). (aai.org)
  • Another viral sequence encoded a protein with 70% amino acid homology to the predicted RdRp. (virology.ws)
  • Differential association of protein subunits with the human RNase MRP and RNase P complexes. (rfam.org)
  • Gupta A, Rath PC (2012) Expression, purification and characterization of the interferon-inducible, antiviral and tumor-suppressor protein, human RNase L. J Biosci 37: 103-111. (jnu.ac.in)
  • It has been shown that human nuclear RNase P is required for the normal and efficient transcription of various small noncoding RNAs, such as tRNA, 5S rRNA, SRP RNA and U6 snRNA genes, which are transcribed by RNA polymerase III, one of three major nuclear RNA polymerases in human cells. (wikipedia.org)
  • The Pyrobaculum and Caldivirga RNase P RNAs are the smallest naturally occurring form yet discovered to function as trans-acting ribozymes. (wikipedia.org)
  • The evolution of the RNase P- and RNase MRP-associated RNAs: phylogenetic analysis and nucleotide substitution rate. (rfam.org)
  • Further, RNase P is one of two known multiple turnover ribozymes in nature (the other being the ribosome), the discovery of which earned Sidney Altman and Thomas Cech the Nobel Prize in Chemistry in 1989: in the 1970s, Altman discovered the existence of precursor tRNA with flanking sequences and was the first to characterize RNase P and its activity in processing of the 5' leader sequence of precursor tRNA. (wikipedia.org)
  • The crystal structure of a bacterial RNase P holoenzyme with tRNA has been recently resolved, showing how the large, coaxially stacked helical domains of the RNase P RNA engage in shape selective recognition of the pre-tRNA target. (wikipedia.org)
  • Ribonuclease P (EC 3.1.26.5, RNase P) is a type of ribonuclease which cleaves RNA. (wikipedia.org)
  • RNA interference (RNAi) is an ancient antiviral response that processes dsRNA and associates it into a nuclease complex that identifies RNA with sequence homology and specifically cleaves it. (scienceopen.com)
  • Isolated eukaryotic and archaeal RNase P RNA has not been shown to retain its catalytic function, but is still essential for the catalytic activity of the holoenzyme. (wikipedia.org)
  • This new DNA-binding motif shares structuralhomology with T4 RNase H, E. coli endonuclease III and Bacillus subtilisDNA polymerase I. The structural homology suggests a mechanism forsubstrate recognition by these enzymes. (embl.de)
  • The overall prised our first group of SARS patients and had a clear his- percentage of amino acid sequence homology between tory of exposure. (cdc.gov)
  • Sequence analysis revealed one of these components to be bovine RNase A, originating from the culture medium and explaining the RNA hydrolyzing activities of Sso7 preparations previously described. (ox.ac.uk)
  • RNase-resistant, noninfectious virus-like particles containing exogenous RNA sequences (armored RNA) are good candidates as RNA controls and standards in RNA virus detection. (politicalespionage.com)
  • Fig. 1 Crystal structures of human -lactal- bumin (PDB file 1HML), lysozyme (2LZT) and ribonuclease A (7RSA) showing potentially long-lived internal water molecules, disul- fide bonds (yellow), and the Ca2+ ion (green) in -lactalbumin. (lu.se)
  • Solution hybridization/ribonuclease protection analysis of total RNA isolated from serum-free monolayer cultures of porcine granulosa cells revealed that insulin (3 μg/ml) increased HMG-CoA messenger RNA (mRNA) concentrations corrected for constitutive 18S ribosomal RNA expression in a time-dependent fashion, with significant effects observed at 12 h and a 6-fold increase by 48 h. (elsevierpure.com)
  • Polyclonal rabbit antibodies raised against recombinant Rpp14 and Rpp29 recognize their corresponding antigens in HeLa cells and precipitate catalytically active RNase P. Rpp29 shows 23% identity with Pop4p, a subunit of yeast nuclear RNase P and the ribosomal RNA processing enzyme RNase MRP. (elsevierpure.com)
  • Rpp14, by contrast, exhibits no significant homology to any known yeast gene. (elsevierpure.com)
  • The N-terminal propeptide region shows homology with bacterial subtilisin. (sigmaaldrich.com)
  • In this work, we have tested the human canonical members of the RNase family using a spot-culture growth inhibition assay based mycobacteria-infected macrophage model for evaluating their anti-tubercular properties. (frontiersin.org)
  • Treatment of HIV-infected activated CD4(+) T cells with a fluorine-derivatized siRNA that is resistant to RNase A yielded similar inhibition of HIV infection. (scienceopen.com)
  • Hier de conclusie van deze review en daaronder een studie abstract met ranpirnasse uit 2002 met bijzonder goede resultaten op de mediane overlevingstijd. (kanker-actueel.nl)
  • Enables poly(A)-specific ribonuclease activity and snRNA binding activity. (nih.gov)
  • Contribution of the enzymatic activity was discarded by site-directed mutagenesis at the RNase catalytic site. (frontiersin.org)
  • Fibrillarin Ribonuclease Activity is Dependent on the GAR Domain and Modulated by Phospholipids. (nih.gov)
  • Identification and analysis of ribonuclease P and MRP RNA in a broad range of eukaryotes. (rfam.org)
  • RNase protection assays and semiquantitative PCR analysis indicated that, although PKCθ transcripts are expressed ubiquitously, the highest levels are found in hematopoietic tissues and cell lines, including T cells and thymocytes. (bgu.ac.il)
  • As serum-free monolayer culture of (swine) granulosa cells offers an in vitro model of hormonally responsive HMG-CoA reductase, we generated a (porcine) complementary DNA and homologous complementary RNA to investigate by sensitive and specific ribonuclease protection assay the hormonal regulation of HMG-CoA reductase gene expression in ovarian cells from immature Graafian follicles. (elsevierpure.com)
  • Using comparative genomics and improved computational methods, a radically minimized form of the RNase P RNA, dubbed "Type T", has been found in all complete genomes in the crenarchaeal phylogenetic family Thermoproteaceae, including species in the genera Pyrobaculum, Caldivirga and Vulcanisaeta. (wikipedia.org)
  • 2009;6: The RNase P family. (rfam.org)
  • Conserved and variable domains of RNase MRP RNA. (rfam.org)
  • Pancreatic ribonuclease also known as ribonuclease A (RNase A) or ribonuclease 1 (RNase1) is an enzyme that catalyzes the breakdown of RNA and plays a role in the digestion of RNA in vertebrate species. (pancreapedia.org)
  • Early work focused on bovine pancreatic RNase because of the large amount present in the pancreas. (pancreapedia.org)
  • The first member to be described is RNase 1 which in addition to being a pancreatic enzyme is produced in a variety of cells including vascular endothelial cells where after secretion it degrades vascular polymeric RNA and has anti-HIV-1 activity (15). (pancreapedia.org)
  • Mammalian ribonuclease inhibitor (RI) is a cytosolic protein of 50 KDa that binds with high affinity and 1:1 stoichiometry to pancreatic ribonuclease thereby inactivating it (8, 17). (pancreapedia.org)
  • One example of such convergence, documented by Zhang (2006) , is the case of the pancreatic RNase in the African colobine monkeys and Asian colobine monkeys (thought to have diverged some 13 million years ago). (evolutionnews.org)
  • RNase P is unique from other RNases in that it is a ribozyme - a ribonucleic acid that acts as a catalyst in the same way that a protein-based enzyme would. (wikipedia.org)
  • Polyclonal rabbit antibodies raised against recombinant Rpp14 and Rpp29 recognize their corresponding antigens in HeLa cells and precipitate catalytically active RNase P. Rpp29 shows 23% identity with Pop4p, a subunit of yeast nuclear RNase P and the ribosomal RNA processing enzyme RNase MRP. (huji.ac.il)
  • Thus, human RNase P differs in the details of its protein composition, and perhaps in the functions of some of these proteins, from the yeast enzyme. (huji.ac.il)
  • Eukaryotic RNase H2 is a heterotrimeric enzyme. (wustl.edu)
  • abstract = "Eukaryotic RNase H2 is a heterotrimeric enzyme. (wustl.edu)
  • RT is a multifunctionnal enzyme which possesses RNA-dependent and DNA-dependent DNA polymerase activities as well as RNase H activity that specifically degrades the RNA strand of RNA-DNA duplexes. (cnrs.fr)
  • The first description of an active form of a recombinant Ty1 enzyme with polymerase and RNase H activities is reported here. (cnrs.fr)
  • This protein appears to be an aldose-1-epimerase-like enzyme (otherwise known as mutarotase) from its homology to bacterial forms of mutarotase. (massey.ac.nz)
  • Isolated eukaryotic and archaeal RNase P RNA has not been shown to retain its catalytic function, but is still essential for the catalytic activity of the holoenzyme. (wikipedia.org)
  • Although the archaeal and eukaryotic holoenzymes have a much greater protein content than the eubacterial ones, the RNA cores from all the three lineages are homologous-helices corresponding to P1, P2, P3, P4, and P10/11 are common to all cellular RNase P RNAs. (wikipedia.org)
  • The catalytic subunit of eukaryotic RNase H2, RNASEH2A, is well conserved and similar to the monomeric prokaryotic RNase HII. (wustl.edu)
  • Unlike Escherichia coli RNase HII, eukaryotic RNase H2 acts processively and hydrolyzes a variety of RNA/DNA hybrids with similar efficiencies, suggesting multiple cellular substrates. (wustl.edu)
  • It has been shown that human nuclear RNase P is required for the normal and efficient transcription of various small noncoding RNAs, such as tRNA, 5S rRNA, SRP RNA and U6 snRNA genes, which are transcribed by RNA polymerase III, one of three major nuclear RNA polymerases in human cells. (wikipedia.org)
  • the RNase H, and a fourth domain, the spacer, which can be partially deleted without any effect on the polymerase activity. (medscape.com)
  • The HBV reverse transciptase polymerase contains five well conserved regions A, B, C, D and E, which show homology with the regions of the reverse transcriptase of HIV and other retroviruses. (medscape.com)
  • Our results suggest that the distance between the RNase H and polymerase active sites corresponds to the length of a 14-nucleotide RNA-DNA heteroduplex. (cnrs.fr)
  • In archaea, RNase P ribonucleoproteins consist of 4-5 protein subunits that are associated with RNA. (wikipedia.org)
  • The structures of protein subunits of archaeal RNase P have been resolved by x-ray crystallography and NMR, thus revealing new protein domains and folding fundamental for function. (wikipedia.org)
  • Five of these protein subunits exhibit homology to archaeal counterparts. (wikipedia.org)
  • These protein subunits of RNase P are shared with RNase MRP, a catalytic ribonucleoprotein involved in processing of ribosomal RNA in the nucleolus. (wikipedia.org)
  • Dive into the research topics of 'Rpp14 and Rpp29, two protein subunits of human ribonuclease P'. Together they form a unique fingerprint. (huji.ac.il)
  • Rpp14, by contrast, exhibits no significant homology to any known yeast gene. (huji.ac.il)
  • Subsequent studies demonstrated that RNase III is also involved in the regulation of gene expression in bacteria and the maturation of non-coding RNAs in eukaryotes. (encyclopedia.pub)
  • The rnc105 mutation was then mapped around 49 min on the chromosome of E. coli , identifying the rnc gene, which was subsequently confirmed to encode Ec-RNase III [ 17 ] . (encyclopedia.pub)
  • Catalytic activity of the recombinant protein was detected only when amino acid residues encoded by the integrase gene were added to the N-terminus of the reverse transcriptase-RNase H domain. (cnrs.fr)
  • The cobC gene codes for a predicted polypeptide of 26 kDa with striking homology to phosphoglycerate mutase, fructose-2,6-bisphosphatase, and acid phosphatase enzymes. (rhea-db.org)
  • The RNAse A superfamily consists of eight "canonical" ribonucleases with enzymatic activity and structural homology to RNase A (15). (pancreapedia.org)
  • Pentatricopeptide repeats of protein-only RNase P use a distinct mode to recognize conserved bases and structural elements of pre-tRNA. (nih.gov)
  • 7. A role of human RNase P subunits, Rpp29 and Rpp21, in homology directed-repair of double-strand breaks. (nih.gov)
  • Additionally, in silico studies such as the prediction of protein structure, homology modeling, and molecular interactions were carried out. (bvsalud.org)
  • Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability. (igred.fr)
  • The overall prised our first group of SARS patients and had a clear his- percentage of amino acid sequence homology between tory of exposure. (cdc.gov)
  • RNase 1 forms dimers by domain swapping of amino termini by sulfhydryl bond formation in such a way as to keep each active site active (18). (pancreapedia.org)
  • Here, we show that the biochemical composition and stoichiometry of the human RNase H2 complex is consistent with the properties previously deduced from genetic studies. (wustl.edu)
  • RNase III was first identified for its role in the initial step of rRNA maturation in the model organism Escherichia coli [ 12 ] . (encyclopedia.pub)
  • Isolation of an RNase III-deficient derivative of E. coli in 1973 [ 15 ] revealed its importance in the initial steps of the maturation of rRNA precursors [ 12 ] . (encyclopedia.pub)
  • In contrast, the RNASEH2B and RNASEH2C subunits from human and Saccharomyces cerevisiae share very little homology, although they both form soluble B/C complexes that may serve as a nucleation site for the addition of RNASEH2A to form an active RNase H2, or for interactions with other proteins to support different functions. (wustl.edu)
  • It also exhibits more limited similarity to other nucleases, RNase H and RuvC. (rcsb.org)
  • homology modelling, comparative analysis , and de novo methods. (biorxiv.org)
  • A combination of homology and de novo methodologies were applied to identify both conserved and novel repeats. (plos.org)
  • One would not expect to see the pervasive occurrence of a high degree of similarity - what would normally be regarded as "homology" - that decidedly cannot be accounted for within the framework of common descent. (evolutionnews.org)
  • Using comparative genomics and improved computational methods, a radically minimized form of the RNase P RNA, dubbed "Type T", has been found in all complete genomes in the crenarchaeal phylogenetic family Thermoproteaceae, including species in the genera Pyrobaculum, Caldivirga and Vulcanisaeta. (wikipedia.org)
  • It is also glycosylated on asparagine residues with the glycosylated form originally termed RNase B. Because of the glycosylation and tertiary structure it runs on most SDS gels at 18 or 19 kDa. (pancreapedia.org)
  • 6. CDYL1 fosters double-strand break-induced transcription silencing and promotes homology-directed repair. (nih.gov)
  • By RNase protection analysis, we identified two major and several minor transcription start-site clusters over a 300-bp region. (nih.gov)
  • It has recently been argued that the archaebacteriium Nanoarchaeum equitans does not possess RNase P. Computational and experimental studies failed to find evidence for its existence. (wikipedia.org)
  • Recent findings also reveal that RNase P has a new function. (wikipedia.org)
  • This crystal structure confirms earlier models of substrate recognition and catalysis, identifies the location of the active site, and shows how the protein component increases RNase P functionality. (wikipedia.org)
  • RNase 4 is present in multiple tissues but it's physiologic role is unclear. (pancreapedia.org)
  • Assuming that Ec-RNase III could have an important role in cell physiology, Kindler et al. (encyclopedia.pub)