RibosomalMammalianRecombinationGenesMembrane transporPathwayHomologStrainsPathogensRecombinantRecAPutativeEscherichiaSecretion systemEukaryoticGenomesStructurallyPeptidoglycanBacteriaInvolved in innate immunityGenomicCharacterizationBRCA2External identifiers for the proteinExtracellularMolecularSolubleHeterologousUnexpectedlyMetabolicSuperfamilyGeneIntracellularPrecursorSubunitCellSpeciesReceptorsFlagellarAnalysis revealReplicationAntigensStructuralAcuteStructuresSequencesSimilaritiesChromosomeApproachInfectionCytokinesHumanYeastImmuneKinase
Ribosomal6
- The ribosomal operons mainly 16S rDNA has proven to be a stable and specific molecular marker for bacterial identification. (ispub.com)
- Ribosomal protein S6 (p-RPS6) is up-regulated in how to buy cheap pentasa online DRG sections stained with anti-RFP (in magenta). (lancasterarchitecture.com)
- p-RPS6, phosphorylated ribosomal protein S6 differentially affects mRNA translation based on unpaired two-tailed t test. (lancasterarchitecture.com)
- Violin plots show distribution of expression levels for 60S ribosomal protein L13a (SMED30019903) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)
- Expression of 60S ribosomal protein L13a (SMED30019903) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells. (stowers.org)
- Violin plots show distribution of expression levels for 60S ribosomal protein L13a (SMED30019903) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells. (stowers.org)
Recombination9
- Streptococcus pneumoniae presents a particularly acute problem in this respect, as it is capable of rapid evolution by homologous recombination with related species. (nih.gov)
- Furthermore, AtRAD54, which performs an important role in the homologous recombination repair pathway, was involved in the pairing of homologous loci with γ-irradiation. (nature.com)
- We found that AtRAD54, which performs an important role in the homologous recombination (HR) repair pathway, was involved in the approach of two homologous loci under γ-irradiation. (nature.com)
- RecA is a widely conserved cytoplasmic protein that performs a range of functions in Escherichia coli, including: DNA repair, homologous recombination, and induction of the SOS pathway. (wisc.edu)
- Botchan's work further indicates that Cdc45 has a structure similar to the bacterial recombination protein RecJ, linking replication and recombination. (biomedcentral.com)
- The breast cancer suppressor protein BRCA2 has an important role in double-strand break repair via homologous recombination. (biomedcentral.com)
- Mutational analysis of BRCA2 by Schlacher and her colleagues enabled the separation of functions required for homologous recombination and for fork protection. (biomedcentral.com)
- Thus, BRCA2 maintains genomic integrity both through homologous recombination and by the protection of nascent strands during DNA replication. (biomedcentral.com)
- To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
Genes6
- Bioinformatics strategies were used to find other homologous genes and proteins, which can also degrade HCH present in various bacteria for controlling of pollution and offer clue to prevent its entry in the food chain. (ispub.com)
- A Drug Target Protein (DTP) Database has been developed having mainly in silico predicted potential drug target proteins and non human homologous genes in bacterial human pathogens. (scialert.net)
- The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)
- The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
- TMs decreased the association of HuR with genes involved in chemotaxis and immune response, including Cxcl10, Il1b and Cd40, reducing their expression and protein secretion in primary murine bone marrow-derived macrophages and in an LPS-induced peritonitis model. (bvsalud.org)
- Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OPP degradation were identified via genomic and proteomic analysis. (nature.com)
Membrane transpor2
- We have determined the sequences of the arabinose-H+ and xylose-H+ membrane transport proteins of Escherichia coli. (ox.ac.uk)
- The major facilitator superfamily (MFS) is a superfamily of membrane transport proteins that facilitate movement of small solutes across cell membranes in response to chemiosmotic gradients. (wikipedia.org)
Pathway3
- Our results suggest that the transient reduction in inter-allelic distance and increase in pairing frequency of homologous loci after DSB result in partial chromatin reorganisation of interphase nuclei and that AtRAD54 contributes to the subcellular movement of homologous loci in the HR repair pathway. (nature.com)
- I'm pretty sure each of those proteins play a very useful role in that pathway. (evcforum.net)
- Currently, her projects mainly focus on studying BRCA2 and Tel1 related protein-protein interactions involved in DNA damage response pathway, using Cryo-EM and other biophysics methods. (imperial.ac.uk)
Homolog2
- Stephen Kowalczykowski (University of California, Davis, USA) described for the first time the purification of this impressive 3,418-amino-acid protein and reported that it binds RAD51 (the human homolog of bacterial RecA) and promotes RAD51 assembly onto single-stranded DNA. (biomedcentral.com)
- The papers are split about evenly on the subject, but I personally find the evidence in favor of (b) to be more compelling (particularly since we know of an actual example of a secretion system evolving from bacterial flagella, namely, the Buchnera homolog of the flagellar system). (evcforum.net)
Strains4
- Recently, 2 atypical, presumably sporadic forms of BSE have been associated with 2 distinct prion strains that are characterized mainly by distinct Western blot profiles of abnormal protease-resistant prion protein (PrP res ), named high-type (BSE-H) and low-type (BSE-L), that also differed from classical BSE. (cdc.gov)
- Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)
- The BLASTP search results showed that the homologous proteins of glycosidases related to raffinose utilization are widely distributed in 196 of the 528 gut bacterial strains. (rsc.org)
- For comparison, recombinant strains of the native bacterial producer S. davaonensis accumulated near one-order less of roseoflavin while no recombinant producers of aminoriboflavin was reported at all. (biomedcentral.com)
Pathogens1
- Currently 10 bacterial pathogens are considered as initials of the database with their general information (disease caused, common symptoms, available drugs etc). (scialert.net)
Recombinant7
- We report here for the first time the in vitro assembly of peptidoglycan using recombinant penicillin-binding proteins from pneumococcus and the precursor lipid II. (nih.gov)
- We checked the expression at the transcript and protein level, cloned the gene and expressed the recombinant protein. (bvsalud.org)
- The X-ray and NMR structural characterization of the recombinant AB-Elavl revealed that the protein maintained the typical ß1α1ß2ß3α2ß4 and three-dimensional organization of eukaryotic RRMs. (bvsalud.org)
- Yeasts have many advantages as biotechnological producers relative to bacteria, however, no recombinant producers of bacterial antibiotics in yeasts are known. (biomedcentral.com)
- Yeast recombinant producers of bacterial antibiotics aminoriboflavin and roseoflavin were constructed and evaluated. (biomedcentral.com)
- The assay uses a recombinant vesicular stomatitis virus expressing NiV glycoprotein and a fluorescent protein. (cdc.gov)
- The recombinant virus propagates as a replication-competent virus in a cell line constitutively expressing NiV fusion protein, but it is restricted to a single round of replication in wild-type cells. (cdc.gov)
RecA1
- Recently, the researchers have discovered that the spatial organization of phospholipids at the curved regions of bacterial cytoplasmic membranes has an important physiological role in bacteria, including the regulation and positioning of the universal enzyme RecA. (wisc.edu)
Putative3
- The mGluRs possess the seven putative transmembrane domains which are characteristic of GPCR proteins. (bdbiosciences.com)
- Of the four putative novel defence proteins (DFPs) annotated by PSI-BLAST three showed similarity to extracellular matrix proteins from vertebrates implicated in innate immunity, while the fourth was similar to, yet distinct from, the anti-microbial protein cecropin. (biomedcentral.com)
- In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
Escherichia1
- Amino-acid sequence homology of a polymorphic cellular protein from human lymphocytes and the chaperonins from Escherichia coli (groEL) and chloroplasts (Rubisco-binding protein). (wikidata.org)
Secretion system1
- Previous work in this area has been on modeling the assembly structure of the type III secretion system and prediction of the high-resolution structures of homo-oligomeric proteins using limited experimental data from Nuclear Magnetic Resonance (NMR) in order to speed up the process of structure determination. (lu.se)
Eukaryotic1
- While the initial MFS structures solved were of bacterial transporters, recently structures of the first eukaryotic structures have been published. (wikipedia.org)
Genomes3
- All bacterial cells must duplicate their genomes prior to dividing into two identical daughter cells. (mdpi.com)
- We also widen the research to the genomes of all the bacteria, finding 227 entries in 12 bacterial phyla. (bvsalud.org)
- Casposons are a recently discovered group of large DNA transposons present in diverse bacterial and ar-chaeal genomes. (hal.science)
Structurally1
- These low and high-resolution data sets originates from T3SSes from different species and can also be collected from other structurally homologous systems, such as the flagella. (lu.se)
Peptidoglycan4
- Animal peptidoglycan recognition proteins homologous to Bacteriophage T3 lysozyme. (embl-heidelberg.de)
- This domain is found in a family of animal peptidoglycan recognition proteins homologous to Bacteriophage T3 lysozyme [ ( PUBMED:9707603 ) ], and some bacterial homologues. (embl-heidelberg.de)
- A peptidoglycan recognition protein in innate immunity conserved frominsects to humans. (embl-heidelberg.de)
- Drosophila Toll is activated by Gram-positive bacteria through a circulating peptidoglycan recognition protein. (sdbonline.org)
Bacteria6
- PCR using the new primer pair on a set of template DNAs of different bacterial genera showed that the method was selective for bacteria belonging to the family Sphingomonadaceae . (ispub.com)
- One bacteria-induced gene encodes a proteinthat, after expression in the baculovirus system, was shown to be apeptidoglycan recognition protein (PGRP). (embl-heidelberg.de)
- Given the urgent need for new antibiotics to combat drug-resistant bacteria, it is logical to evaluate whether or not unexploited bacterial processes, such as orisome assembly, should be more closely examined for sources of novel drug targets. (mdpi.com)
- Bacterial recognition by PGRP-SA and downstream signalling by Toll/DIF sustain commensal gut bacteria in Drosophila. (sdbonline.org)
- In GRAM NEGATIVE BACTERIA, multiprotein complexes that function to translocate pathogen protein effector molecules across the bacterial cell envelope, often directly into the host. (bvsalud.org)
- Several of the systems have homologous components functioning similarly in GRAM POSITIVE BACTERIA. (bvsalud.org)
Involved in innate immunity1
- From our results and the sequence homology, weconclude that PGRP is a ubiquitous protein involved in innate immunity,conserved from insects to humans. (embl-heidelberg.de)
Genomic2
- Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
- This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. (embl-heidelberg.de)
Characterization1
- For bacterial in vivo characterization, we evaluate the biological relevance of our targets in the host-microbe interaction: we use genome site directed mutagenesis, homologous and heterologous complementation with wild-type and mutant versions of the proteins, classical microbiological determination assays (i.e. biofilm formation, EPS content, flagellar apparatus production, motility, survival to stresses, growth, etc). (leloir.org.ar)
BRCA21
- The purification of BRCA2 will allow the study of the functions of the different domains in this large protein. (biomedcentral.com)
External identifiers for the protein1
- List of external identifiers for the protein from various database sources. (yeastgenome.org)
Extracellular2
- The two halves of the protein pack against each other in a clam-shell fashion, sealing via interactions at the ends of the transmembrane helices and extracellular loops. (wikipedia.org)
- The sizable extracellular N-terminal domain is homologous to bacterial periplasmic binding proteins and serves as the glutamate binding site. (bdbiosciences.com)
Molecular4
- We characterized 5 atypical BSE-H isolates by analyzing their molecular and neuropathologic properties during transmission in transgenic mice expressing homologous bovine prion protein. (cdc.gov)
- Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
- Understanding the molecular basis of bacterial cell wall assembly is of paramount importance in addressing the threat of increasing antibiotic resistance worldwide. (nih.gov)
- A model is proposed where the relative levels of active antagonist (RsbV1) and switch-protein anti-sigma factor (RsbW) control the availability of σ 66 and subsequently act as a molecular 'throttle' for Chlamydia growth and development. (plos.org)
Soluble3
- Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)
- Biochemical fractionation showed that IglA is a soluble cytoplasmic protein and immunoprecipitation experiments demonstrate that it interacts with the downstream-encoded IglB. (biomedcentral.com)
- A soluble C1b protein and its regulation of soluble type 7 adenylyl cyclase. (uchicago.edu)
Heterologous1
- A flavin-dependent monoxygenase (OppA1), one of the most up-regulated proteins in the OPP-growing cells, was isolated via heterologous expression and its catabolic activity was verified in vitro . (nature.com)
Unexpectedly1
- They are homologous with each other and, unexpectedly, with the glucose transporters of human hepatoma and rat brain cells. (ox.ac.uk)
Metabolic1
- Our conceptual approach is to identify protein targets involved in bacterial sensory or metabolic pathways important in the infectious process and to describe their function both at the atomic level and at the host-microbe interaction scale. (leloir.org.ar)
Superfamily2
- The major facilitator superfamily (MFS) are membrane proteins which are expressed ubiquitously in all kingdoms of life for the import or export of target substrates. (wikipedia.org)
- MazG is a homodimeric alpha-helical protein that belongs to the superfamily of all-alpha NTP pyrophosphatases. (shengsci.com)
Gene3
- [ 4 ] The emm gene encodes the M protein. (medscape.com)
- IglA is a 21 kDa protein encoded by a gene that is part of an iglABCD operon located on the Francisella pathogenicity island (FPI). (biomedcentral.com)
- Choose Gene Evolution libraries for screening projects to improve the properties of your protein of interest. (eurofinsgenomics.eu)
Intracellular2
- Coupling with G proteins provides the metabotropic glutamate receptors (mGluRs) with the capacity for intracellular signal transduction. (bdbiosciences.com)
- The second intracellular loop of metabotropic glutamate receptor 1 cooperates with the other intracellular domains to control coupling to G-proteins. (bdbiosciences.com)
Precursor3
- The MCM2-7 protein complex is the precursor of the replicative helicase that unwinds DNA in front of the active replication complex. (biomedcentral.com)
- The effects of RoF and its immediate biosynthetic precursor AF on the bacterial growth were studied. (biomedcentral.com)
- Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)
Subunit2
- This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
- In particular, we are interested in a subclass of protein assemblies, homomeric assemblies, which are produced by the repetition of single type of subunit. (lu.se)
Cell10
- As originally described by Lancefield, beta-hemolytic streptococci can be divided into many groups based on the antigenic differences in group-specific polysaccharides located in the bacterial cell wall. (medscape.com)
- BacA is a bacterial lysin from Enterococcus faecalis that degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. (nih.gov)
- The flagellum is an ion-powered rotary motor, anchored in the membranes surrounding the bacterial cell. (biblestudymanuals.net)
- One live-cell imaging technique, a chromatin-tagging system that is based on the bacterial operator/repressor system, has been a powerful technique for analysing chromatin dynamics and arrangement in real time 11 , 12 . (nature.com)
- We revealed that the homologous loci kept a constant three-dimensional distance in the nucleus using live-cell imaging with a bacterial operator/repressor system. (nature.com)
- Since the flagellar filament is external to the cell membrane, this poses an engineering problem: namely, how can we design the flagellum such that a large number of proteins assemble outside the cell? (evcforum.net)
- Studies have demonstrated that food allergens are transported in large quantities across the epithelium by binding to cell surface IgE/CD23, which opens a gate for intact dietary allergens to transcytose across the epithelial cells that protect the antigenic protein from lysosomal degradation in enterocytes. (medscape.com)
- Non-IgE mediated food allergies involve T-cell mediated immunity to certain food proteins. (medscape.com)
- He is working on developing a workflow for prototyping proteins using cell-free systems and machine learning. (imperial.ac.uk)
- This macromolecular assembly comprises more than 20 conserved proteins that form a series of ring-like structures and a needle-shaped protrusion, which allows the transport of proteins into a host cell. (lu.se)
Species1
- Information about cross-species functional complementation between yeast and other species, curated by SGD and the Princeton Protein Orthology Database (P-POD). (yeastgenome.org)
Receptors2
- Eight metabotropic glutamate receptors (mGluR1-8) and several Ca[2+] sensing receptors belong to a novel G-protein coupled receptor (GPCR) family. (bdbiosciences.com)
- [ 3 ] Genetic variations in receptors for bacterial products are likely to be related to allergic sensitizations. (medscape.com)
Flagellar1
- This schematic diagram highlights the assembly process of the bacterial flagellar filament and the cap-filament complex. (biblestudymanuals.net)
Analysis reveal2
- Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source. (frontiersin.org)
- Protein-protein docking and analysis reveal that two homologous bacterial adenylyl cyclase toxins interact with calmodulin differently. (uchicago.edu)
Replication2
- Chromosome replication is triggered when a nucleoprotein complex, termed the orisome, assembles, unwinds the duplex DNA, and recruits the proteins required to establish new replication forks. (mdpi.com)
- Obviously, the initiation of chromosome replication is essential to bacterial reproduction, but this process is not inhibited by any of the currently-used antimicrobial agents. (mdpi.com)
Antigens1
- Many food proteins can act as antigens in humans. (medscape.com)
Structural8
- Environmental sequences of ten novel plastid lineages and structural innovations in plastid proteins confirm that plastids in apicomplexans and their relatives are widespread and share a common, photosynthetic origin. (elifesciences.org)
- Her work here is focussed on providing structural and mechanistic insight into the activity of diadenylate cyclase DacA, an essential membrane protein responsible for the production of c-di-AMP in Staphylococcus aureus . (imperial.ac.uk)
- Luke completed his D.Phil in Clinical Medicine at Oxford University (Merton College), but his research career has predominantly focused on investigating protein function using structural biology as a means to provide a mechanistic understanding in life and in disease. (imperial.ac.uk)
- The fast sulfide association rate constant can be related to the presence of Gln(64)E7, as the heme distal residue, together with the protein structural properties in the CD-E distal region. (shengsci.com)
- Structural analysis of the rate-limiting transition states in the folding of Im7 and Im9: similarities and differences in the folding of homologous proteins. (shengsci.com)
- Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein. (shengsci.com)
- Fasciculin 2 and toxin alpha proteins belong to the same structural family of three-fingered snake toxins. (shengsci.com)
- Our goal is to use high-resolution structural modeling techniques guided by constraints taken from lower resolution experimental data to generate structural models of important biological protein assemblies for which high resolution structural determination is unfeasible. (lu.se)
Acute2
- A ubiquitous organism, S pyogenes is the most common bacterial cause of acute pharyngitis , accounting for 15-30% of cases in children and 5-10% of cases in adults. (medscape.com)
- PSA levels are usually elevated in men with acute bacterial prostatitis. (medscape.com)
Structures5
- The first structures were of the glycerol 3-phosphate/phosphate exchanger GlpT and the lactose-proton symporter LacY, which served to elucidate the overall structure of the protein family and provided initial models for understanding the MFS transport mechanism. (wikipedia.org)
- This shift has been made possible in part by a growing number of solved protein structures, often in complex with DNA, and elegant single-molecule approaches. (biomedcentral.com)
- Many biological processes are controlled by large multi-component protein assemblies, the size and complexity of which typically precludes the determination of their high-resolution structures. (lu.se)
- Currently we are working with development of methods to determine structures of fibrillar proteins by a combination of structure modeling and X-ray fiber diffraction interference. (lu.se)
- These effectors are involved in producing surface structures for adhesion, bacterial motility, manipulation of host functions, modulation of host defense responses, and other functions involved in facilitating survival of the pathogen. (bvsalud.org)
Sequences1
Chromosome1
- For integration into the host chromosome , casposons employ an endonuclease that is homologous to the Cas1 protein involved in proto-spacer integration by the CRISPR-Cas adaptive immune system. (hal.science)
Approach2
- We also found that DNA double-strand breaks (DSBs) induce the approach of the homologous loci with γ-irradiation. (nature.com)
- These results suggest that homologous loci approach each other to repair DSBs and AtRAD54 mediates these phenomena. (nature.com)
Infection2
- Chlamydia trachomatis is the leading cause of both bacterial sexually transmitted infection and infection-derived blindness world-wide. (plos.org)
- [ 1 , 2 ] The clinical picture is characterized by marked leukocytosis and localized bacterial infections that are difficult to detect until they have progressed to an extensive level secondary to lack of leukocyte recruitment at the site of infection. (medscape.com)
Cytokines1
- HuR (ELAVL1) is an RNA-binding protein that regulates cytokines and chemokines transcripts containing AU/U-rich elements (AREs) and mediates the LPS-induced response. (bvsalud.org)
Human1
- Here, we browsed the A. baumannii proteome for homologous proteins to the human HuR(ELAVL1), an RNA binding protein containing three RRMs. (bvsalud.org)
Yeast1
- However, it has previously been shown that deletion of the amino-terminal part of the Pol2 protein containing the Pol ε catalytic domain resulted in sick but viable yeast cells, while deletion of the carboxy-terminal part of Pol2 was lethal. (biomedcentral.com)
Immune2
- We also identified several unknown proteins, some of which could have probable immune-related functions based on the results of the ProDom analysis. (biomedcentral.com)
- In this study, we have constructed and analysed an immune transcriptome following bacterial challenge of the Indian tasar wild silkmoth, Antheraea mylitta , an economically important lepidopteran cultivated for silk production. (biomedcentral.com)
Kinase1
- Mitogen-activated protein kinase p38alpha and retinal ischemic preconditioning. (uchicago.edu)