The FPIA method and the two remaining HPLC methods (HPLC with fluorescence detection using either TCEP or TBP as a reducing agent and SBD-F as a labeling agent) showed no deviation compared with results of the GC-MS method. (cdc.gov)
Elevated plasma homocysteine (Hcy), generally defined as fasting plasma Hcy levels greater than 15 µmol/L, is an independent risk factor for vascular diseases (1,2). (cdc.gov)
The dosage of N -acetylcysteine used in the current study was identical to that used by Scholze and colleagues, [ 19 ] and in the range that has been reported to efficiently reduce plasma homocysteine levels in healthy subjects. (medscape.com)
Abstract The present study was designed to evaluate the strength of association of raised plasma homocysteine concentration as a risk factor for coronary heart disease independent of conventional risk factor. (bvsalud.org)
Plasma homocysteine in polycystic ovary syndrome: does it correlate with insulin resistance and ethnicity? (unboundmedicine.com)
To compare total plasma homocysteine (Hcy) in PCOS with controls from ethnic groups at high and low risk of insulin resistance. (unboundmedicine.com)
Fasting total plasma Hcy concentration was analysed by fluorescence polarization immunoassay and examined for any correlation with age, body mass index (BMI), central obesity, fasting insulin and insulin sensitivity [calculated by the Quantitative Insulin Sensitivity Check Index (QUICKI) method], lipids and testosterone in each ethnic group. (unboundmedicine.com)
Elevation of fasting plasma homocysteine in PCOS varies with ethnicity and correlates significantly with fasting insulin. (unboundmedicine.com)
For example, serum folate is a good indicator of folate intake, red blood cell (RBC) folate is a good status indicator, and plasma total homocysteine is a good functional indicator of one-carbon metabolism. (cdc.gov)
Correlation1
The correlation between reduction of homocysteine and change in pulse pressure was analysed using linear regression. (medscape.com)
Outcomes1
The objective was to determine the effect of different cutoff choices on outcomes and of the different degrees of serum vitamin B-12 status, definable by the concurrent use of a functional and circulating marker as the first steps to developing a data-based consensus on the biochemical diagnosis of vitamin B-12 deficiency. (nih.gov)
Total1
Total 600 serum and 600 swab samples were collected, 200 from each broiler, layers and breeders poultry in Rawalpindi and Abbottabad districts. (bvsalud.org)
Levels1
These data will be used to estimate deficiencies and toxicities of specific nutrients in the population and subgroups, to provide population reference data, and to estimate the contribution of diet, supplements, and other factors to serum levels of nutrients. (cdc.gov)
Two of the HPLC methods (HPLC with electrochemical detection and HPLC with fluorescence detection using sodium borohydride as a reducing agent and monobromobimane as a labeling agent) and the EIA method produced results that were, on average, 7.5%, 8.1%, and 7.4% higher than GC-MS results. (cdc.gov)
Groups1
Differences between groups were analysed using the Wilcoxon Mann-Whitney test (homocysteine data) and the two-sample t-test (haemodynamic/pulse pressure data). (medscape.com)
Purposes1
Laboratories that used HPLC were further subdivided based on the type of detection each laboratory used (electrochemical or fluorescence) and by each of the types of reducing and labeling reagent each used to convert protein-bound and oxidized Hcy into free Hcy and to attach a fluorescent tag to the free Hcy for detection purposes. (cdc.gov)