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Acyl Carrier ProteinCarrier ProteinsApoproteinsFatty Acid Synthase, Type II3-Oxoacyl-(Acyl-Carrier-Protein) SynthasePantetheineHoloenzymesApoenzymesMolecular Sequence DataAmino Acid SequenceEscherichia coliAcyl-Carrier Protein S-MalonyltransferaseEnoyl-(Acyl-Carrier-Protein) Reductase (NADH)Carrier StateModels, MolecularProtein BindingBacterial Proteins3-Oxoacyl-(Acyl-Carrier-Protein) ReductaseGram-Negative Chemolithotrophic BacteriaProtein ConformationTransferases (Other Substituted Phosphate Groups)TriclosanBinding SitesKineticsAcyltransferasesPolyketide SynthasesCarbon-Sulfur LigasesProtein Structure, TertiaryCrystallography, X-RayFatty Acid SynthasesSequence Homology, Amino AcidDrug CarriersReduced Folate Carrier ProteinBase SequenceFatty AcidsSubstrate SpecificityCloning, MolecularCoenzyme ARecombinant ProteinsThiolester HydrolasesMutationProtein Structure, SecondaryMembrane Transport ProteinsEnoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific)Heterozygote DetectionLigasesMitochondrial ADP, ATP TranslocasesAcylationMembrane ProteinsCarbon-Nitrogen LigasesAcetyl-CoA C-AcetyltransferaseProtein FoldingSequence AlignmentPeptide SynthasesCeruleninPantothenic AcidEscherichia coli ProteinsHeterozygoteBiological TransportCircular DichroismMalonyl Coenzyme ABiotinAcetyl-CoA CarboxylaseTemperatureMalonatesPlant ProteinsGalactosidesRecombinant Fusion ProteinsElectrophoresis, Polyacrylamide GelGenetic Complementation TestMitochondriaStreptomycesMolecular WeightLigandsDNA PrimersProtein DenaturationDNA, ComplementaryRNA, MessengerVaccines, ConjugateCell LineUbiquitin-Conjugating EnzymesHaptensProton-Coupled Folate TransporterCell MembraneAcetyl Coenzyme AMultigene FamilySpectrometry, Mass, Electrospray IonizationProtein TransportPlasmidsCoriandrumProteinsThermodynamicsCalorimetry, Differential ScanningHemocyaninMultienzyme ComplexesSaccharomyces cerevisiaeMitochondrial ProteinsModels, BiologicalDicarboxylic Acid TransportersSterols
SynthaseAcyl carrierACPSLyaseEscherichiaEnzymeTransferrinTransferaseTranscobalaminSynthetaseSequencesTUMOR PROTEINFattyCharacterizationPeptidylGenePhosphopantetheineReactionComplexFunctionalInteractionsMembraneConformationalStructuresAnticancerBoundExperimental DataSpeciesBiochemicalReliesFormTransferasesFamilyInteractionActivityCellularDynamicsStructureDomains
Synthase8
  • In enzymology and molecular biology, a holo-[acyl-carrier-protein] synthase (ACPS, EC 2.7.8.7) is an enzyme that catalyzes the chemical reaction: CoA-[4'-phosphopantetheine] + apo-acyl carrier protein ⇌ {\displaystyle \rightleftharpoons } adenosine 3',5'-bisphosphate + holo-acyl carrier protein This enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. (wikipedia.org)
  • Other names in common use, disregarding the synthetase/synthase spelling difference, include acyl carrier protein holoprotein synthetase, holo-ACP synthetase, coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine, acyl carrier protein synthetase (ACPS), PPTase, acyl carrier protein synthase, P-pant transferase, and CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase. (wikipedia.org)
  • Dihydromonacolin L acid is synthesized while bound to an acyl-carrier protein domain of the lovastatin nonaketide synthase (EC 2.3.1.161 ). (genome.jp)
  • citrate lyase holo-[acyl-carrier protein] synthase (EC 2.7.7.61). (lbl.gov)
  • Both the precursor and mature forms are substrates for phosphopantetheine attachment by a soluble chloroplast holo-acyl carrier protein synthase. (uchicago.edu)
  • Acyl carrier protein (ACP) import into chloroplasts does not require the phosphopantetheine: evidence for a chloroplast holo-ACP synthase. (uchicago.edu)
  • Cpr37, which belongs to the adenylation domain protein in the nonribosomal peptide synthase (NRPS), subsequently activates PABA and loads it to holo-Cpr36 Two proteins Cpr34 and Cpr35 work in concert to catalyze decarboxylative condensation between a thioester linked PABA and malonyl- S -acyl carrier protein (ACP) during aromatic polyketide biosynthesis catalyzed by type II polyketide synthases. (uky.edu)
  • We found that sphingomyelin is enriched in migrasomes and identified sphingomyelin synthase 2 (SMS2) as an essential protein for migrasome biogenesis. (bvsalud.org)
Acyl carrier14
  • It transfers a 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to an invariant serine in an acyl carrier protein (ACP), a small protein responsible for acyl group activation in fatty acid biosynthesis. (wikipedia.org)
  • The systematic name of this enzyme class is CoA-[4'-phosphopantetheine]:apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase. (wikipedia.org)
  • Acyl carrier protein. (wikipedia.org)
  • Acyl carrier proteins are critical components of fatty acid and polyketide biosynthesis. (rsc.org)
  • Selectivity for the holo over apo -form of acyl carrier proteins is demonstrated indicating that only the phosphopantetheine - thiol is modified. (rsc.org)
  • UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (EC 2.3.1.191, UDP-3-O-acyl-glucosamine N-acyltransferase, UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase, acyltransferase LpxD, acyl-ACP:UDP-3-O-(3-hydroxyacyl)-GlcN N-acyltransferase, firA (gene), lpxD (gene)) is an enzyme with systematic name (3R)-3-hydroxymyristoyl-(acyl-carrier protein):UDP-3-O-((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine N-acetyltransferase. (wikipedia.org)
  • 3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-3-O-((3R)- hydroxytetradecanoyl)-alpha-D-glucosamine = UDP-2-N,3-O-bis((3R)-3- hydroxytetradecanoyl)-alpha-D-glucosamine + a holo-[acyl-carrier- protein]. (cathdb.info)
  • LpxD from Escherichia coli prefers (R,S)-3-hydroxymyristoyl-[acyl- carrier protein] over (R,S)-3-hydroxypalmitoyl-[acyl-carrier protein]. (cathdb.info)
  • Acyl carrier protein import into chloroplasts. (uchicago.edu)
  • Lipoate protein ligase B primarily recognizes the C8-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein. (du.ac.in)
  • Catalyzes the formation of 2-(5''-triphosphoribosyl)-3'- dephosphocoenzyme-A, the precursor of the prosthetic group of the holo- acyl carrier protein (gamma chain) of citrate lyase, from ATP and dephospho-CoA. (string-db.org)
  • Transfers 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A on a serine residue to the apo-acyl carrier protein (gamma chain) of the citrate lyase to yield holo-acyl carrier protein. (string-db.org)
  • Activation of the acyl carrier protein (ACP) domain of the SwfA protein to its holo-form by co-expression with Svp is the first functional proof of swf type genes in marine sponges. (cirn-na.com)
  • Tries to crystallize the YchM STAS area resulted in spontaneous co-crystallization with acyl carrier proteins (ACP) liganded with malonyl coA (Babu et al. (exposed-skin-care.net)
ACPS2
  • All ACPS enzymes known so far are evolutionally related to each other in a single superfamily of proteins. (wikipedia.org)
  • This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP. This superfamily consists of two subtypes: the trimeric ACPS type such as E. coli ACPS and the monomeric Sfp (PCP-synthesizing) type such as B. subtilis SFP. (wikipedia.org)
Lyase1
  • Cpr12, encoded by a gene that was originally proposed to be outside the gene cluster and sharing similarity to proteins annotated as ADC lyase, was revealed to catalyze the elimination of pyruvate to form PABA. (uky.edu)
Escherichia1
Enzyme4
  • The adenylation domain of the enzyme catalyzes the activation of L-arginine to (L-arginyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. (expasy.org)
  • GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)
  • Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
  • In the human body, iron is present in all cells and has several vital functions -- as a carrier of oxygen to the tissues from the lungs in the form of hemoglobin (Hb), as a facilitator of oxygen use and storage in the muscles as myoglobin, as a transport medium for electrons within the cells in the form of cytochromes, and as an integral part of enzyme reactions in various tissues. (cdc.gov)
Transferrin1
  • PurMa TM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage. (purmabiologics.com)
Transferase1
  • We further demonstrate that this conformational diversity is an essential prerequisite for the directed movement of the 4'-PP cofactor and its interaction with externally acting proteins such as thioesterases and 4'-PP transferase. (rcsb.org)
Transcobalamin1
  • In a pilot randomized controlled trial of the Mediterranean diet (MedDiet) and MET, whose participants were characterized by different components of metabolic syndrome, we tested the effect of MET on serum levels of B 12 , holo transcobalamin II (holo-TC-II), and methylmalonic acid (MMA). (mdpi.com)
Synthetase2
  • Inherent to synthetase chemistry is the thioester templated mechanism that relies on protein/protein interactions and interdomain dynamics. (rcsb.org)
  • Here we report the structure of the epimerization domain/peptidyl carrier protein didomain construct from the first module of the cyclic peptide antibiotic gramicidin synthetase. (rcsb.org)
Sequences1
  • Open up in another window Physique 1 Phylogenetic romantic relationship of BIIE 0246 supplier human being SLC26 polypeptides generated with Jalview (http://www.jalview.org) using NCBI proteins sequences listed in Desk 1. (exposed-skin-care.net)
TUMOR PROTEIN1
  • Description: Quantitativesandwich ELISA kit for measuring Human p53/tumor protein, p53/TP53 in samples from serum, cell culture supernates, urine, cerebrospinalfluid (CSF), tissue homogenates, cell lysates. (ksiazkiwnauce.pl)
Fatty1
  • Built YchM deletion exhibited artificial lethality with multiple fatty acidity biosynthesis genes, as well as the YchM polypeptide bodily interacted numerous proteins from the fatty acidity biosynthetic pathway. (exposed-skin-care.net)
Characterization1
  • Here we present the functional assignment and biochemical characterization of seven proteins, Cpr33-38 and Cpr12, that initiate the biosynthesis of the polyamide. (uky.edu)
Peptidyl1
  • The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. (expasy.org)
Gene2
  • There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Bacillus subtilis genome using these genes and the sgRNAs available from the Bacillus Genetic Stock Center. (freegenes.org)
  • Phylogenetic analysis of the cif protein, which is encoded by the cif gene, shows that the evolution of cifA and cifB is related to each other. (biomedcentral.com)
Phosphopantetheine1
  • Both apo-PCP (without the bound 4'-phosphopantetheine cofactor) and holo-PCP exist in two different stable conformations. (rcsb.org)
Reaction1
  • In 1992, Saito and co-workers showed that the reaction of holo-NCS with small thiols, such as β-mercaptoethanol (BME), takes a different course, to form a product that is formally a 1:1:1 adduct of thiol, 1, and water. (caltech.edu)
Complex1
  • Structures of the protein-chromophore complex and the apoprotein form of neocarzinostatin were determined at 1.8 angstrom resolution. (caltech.edu)
Functional1
  • Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. (purmabiologics.com)
Interactions3
  • In drug discovery, efficient screening of protein-drug interactions (PDIs) is hampered by the limitations of current biophysical approaches. (nature.com)
  • Using this YaxAB nanopore, we demonstrate label-free, single-molecule detection of interactions between the anticancer Bcl-xL protein and small-molecule drugs as well as the Bak-BH3 peptide. (nature.com)
  • Although protein-protein interactions (PPIs) are promising targets, drugging them is one of the key challenges in drug discovery 19 . (nature.com)
Membrane1
  • membrane associated protein [Ensembl]. (ntu.edu.sg)
Conformational1
  • The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
Structures1
  • Both holo (phosphopantethiene post-translationally modified) and apo structures were determined, each representing catalytically relevant conformations of the two domains. (rcsb.org)
Anticancer1
  • Oncogenic Ras genes and the G-proteins which they encode are important targets for anticancer research. (caltech.edu)
Bound1
  • The chromophore displays marked nonlinearity of the triple bonds and is bound noncovalently in a pocket formed by the two protein domains. (caltech.edu)
Experimental Data1
  • Hits to curated proteins without experimental data as to their function are never considered high confidence. (lbl.gov)
Species1
  • P. knowlesi carriers were significantly younger than those infected with other malaria parasite species. (cdc.gov)
Biochemical1
  • Their ability to function as potent inhibitors of oligosaccharide synthesis in eukaryotic cells has established them as unique biochemical probes of the role of glycosylation on protein structure and function. (caltech.edu)
Relies1
  • GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)
Form3
  • We show that one of the apo conformations and one of the holo conformations are identical, whereas the two remaining conformations are only detectable by nuclear magnetic resonance spectroscopy in either the apo or holo form. (rcsb.org)
  • Cpr36 is demonstrated to function as a free-standingpeptidyl carrier protein (PCP), which is activated to form holo-protein from the apo-form. (uky.edu)
  • In their oncogenic form, the natural GTP-ase activity of Ras proteins is inhibited, leading to overstimulation of the signaling pathway for cell growth. (caltech.edu)
Transferases1
  • 4'-Phosphopantetheinyl transferases (PPTases) play an essential role in activating the carrier protein domains of mega-synthases involved in primary and secondary metabolism and have been validated as promising drug targets in multiple pathogens. (bvsalud.org)
Family3
  • Uncharacterised protein family UPF0052 [Interproscan]. (ntu.edu.sg)
  • Conserved hypothetical protein, UPF0052 family. (ntu.edu.sg)
  • UPF0178 family protein. (string-db.org)
Interaction1
  • The therapeutic efficacy of drugs is mediated by physical interaction with their cognate targets (mainly proteins). (nature.com)
Activity2
  • Neocarzinostatin is composed of a labile chromophore with DNA-cleaving activity and a stabilizing protein. (caltech.edu)
  • The transportation activity of the holoproteins is not expressed, however they are presumed to provide as HCO3? (exposed-skin-care.net)
Cellular1
Dynamics1
  • Protein dynamics plays an important role in protein function. (rcsb.org)
Structure2
  • CATH: Protein Structure Classification Database by I. Sillitoe, N. Dawson, T. Lewis, D. Lee, J. Lees, C. Orengo is licensed under a Creative Commons Attribution 4.0 International License . (cathdb.info)
  • The long funnel-shaped structure and nanofluidic characteristics of the YaxAB nanopore enable the electro-osmotic trapping of diverse folded proteins and high-resolution monitoring of PDIs. (nature.com)
Domains1
  • Small molecules capable of acylating this prosthetic group will provide a simple and reversible means of introducing novel functionality onto carrier protein domains. (rsc.org)