• citrate lyase holo-[acyl-carrier protein] synthase (EC 2.7.7.61). (lbl.gov)
  • Catalyzes the formation of 2-(5''-triphosphoribosyl)-3'- dephosphocoenzyme-A, the precursor of the prosthetic group of the holo- acyl carrier protein (gamma chain) of citrate lyase, from ATP and dephospho-CoA. (string-db.org)
  • Transfers 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A on a serine residue to the apo-acyl carrier protein (gamma chain) of the citrate lyase to yield holo-acyl carrier protein. (string-db.org)
  • Cpr12, encoded by a gene that was originally proposed to be outside the gene cluster and sharing similarity to proteins annotated as ADC lyase, was revealed to catalyze the elimination of pyruvate to form PABA. (uky.edu)
  • Cpr37, which belongs to the adenylation domain protein in the nonribosomal peptide synthase (NRPS), subsequently activates PABA and loads it to holo-Cpr36 Two proteins Cpr34 and Cpr35 work in concert to catalyze decarboxylative condensation between a thioester linked PABA and malonyl- S -acyl carrier protein (ACP) during aromatic polyketide biosynthesis catalyzed by type II polyketide synthases. (uky.edu)
  • Other names in common use, disregarding the synthetase/synthase spelling difference, include acyl carrier protein holoprotein synthetase, holo-ACP synthetase, coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine, acyl carrier protein synthetase (ACPS), PPTase, acyl carrier protein synthase, P-pant transferase, and CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase. (wikipedia.org)
  • The human 4'-phosphopantetheine transferase is also capable of phosphopantetheinylation of peptidyl carrier and acyl carrier proteins from prokaryotes. (rhea-db.org)
  • In enzymology and molecular biology, a holo-[acyl-carrier-protein] synthase (ACPS, EC 2.7.8.7) is an enzyme that catalyzes the chemical reaction: CoA-[4'-phosphopantetheine] + apo-acyl carrier protein ⇌ {\displaystyle \rightleftharpoons } adenosine 3',5'-bisphosphate + holo-acyl carrier protein This enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. (wikipedia.org)
  • All ACPS enzymes known so far are evolutionally related to each other in a single superfamily of proteins. (wikipedia.org)
  • This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP. This superfamily consists of two subtypes: the trimeric ACPS type such as E. coli ACPS and the monomeric Sfp (PCP-synthesizing) type such as B. subtilis SFP. (wikipedia.org)
  • The enzyme is capable of transferring the 4'-phosphopantetheine moiety of coenzyme A to a conserved serine residue in both the acyl carrier protein domain of the human cytosolic multifunctional fatty acid synthase and the acyl carrier protein associated independently with human mitochondria. (rhea-db.org)
  • The codon table used was the FreqB table from " Design Parameters to Control Synthetic Gene Expression in Escherichia coli ", which was used because it was shown to express proteins well and was recommended to us by JCVI. (freegenes.org)
  • It transfers a 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to an invariant serine in an acyl carrier protein (ACP), a small protein responsible for acyl group activation in fatty acid biosynthesis. (wikipedia.org)
  • In this scenario, hydride is predicted to be added to the re face to generate the S configuration resulting in the same stereochemical outcome as other 3-oxoacyl-ACP reductase (FabG) from bacterial type II fatty acid synthases.These findings are critical advancement for interrogating the biosynthesis of the unusual chemical components of the family of antibiotics of capuramycin. (uky.edu)
  • The systematic name of this enzyme class is CoA-[4'-phosphopantetheine]:apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase. (wikipedia.org)
  • GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)
  • Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
  • The human enzyme, which is expressed mainly in the cytosolic compartment in a wide range of tissues, is a 329-residue, monomeric protein. (rhea-db.org)
  • It is a conjecture that the ε subunit regulates ATP hydrolytic function of the F1Fo ATP synthase in bacteria. (tudelft.nl)
  • Here we present the functional assignment and biochemical characterization of seven proteins, Cpr33-38 and Cpr12, that initiate the biosynthesis of the polyamide. (uky.edu)
  • Functional characterization of Cpr38, which has sequence similarity to the gene products encoded by pabA and pabB from E. coli , revealed that it functions as a 4-amino-4-deoxychorismate (ADC) synthase catalyzing a two-step reaction involving amidohydrolysis of L-Gln with ammonia channeled and incorporated into chorismic acid to generate ADC. (uky.edu)
  • There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Bacillus subtilis genome using these genes and the sgRNAs available from the Bacillus Genetic Stock Center. (freegenes.org)
  • GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)
  • Functional reintegration into lipid environments represents a major challenge for in vitro investigation of integral membrane proteins (IMPs). (tudelft.nl)
  • Hits to curated proteins without experimental data as to their function are never considered high confidence. (lbl.gov)
  • Cpr36 is demonstrated to function as a free-standingpeptidyl carrier protein (PCP), which is activated to form holo-protein from the apo-form. (uky.edu)
  • Search proteins in UniProtKB for this molecule. (rhea-db.org)
  • High yield purification of Ulp1 is required during the isolation and purification of SUMO-tagged recombinant proteins. (bvsalud.org)
  • The enterocin enzymes holo-EncC26 EncA-EncB26 EncD6 and EncN27 from had been ready as His-tagged recombinant proteins as previously defined6 26 The plasmid encoding FabD was supplied by Teacher K. A. Reynolds. (cell-metabolism.com)
  • 14478 3-oxoacyl-[acyl-carrier protein] reductase fabG BBZA01000001 CDS ARMA_0014 complement(14430. (go.jp)
  • Recently discovery appearance and biochemical analyses from the enterocin biosynthetic gene cluster including reconstitution from the metabolic pathway confirmed further participation of the sort II polyketide synthase EncABC as well as the NADPH-dependent reductase EncD6 7 9 (Fig. 1). (cell-metabolism.com)
  • While type II polyketide synthase pathways typically produce polycyclic aromatic items just like the antibiotic tetracycline as well as the SCH 54292 anticancer agent doxorubicin10 aromatic polyketides known as wailupemycins are produced only as minimal items from the enterocin biosynthetic pathway7. (cell-metabolism.com)
  • It transfers a 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to an invariant serine in an acyl carrier protein (ACP), a small protein responsible for acyl group activation in fatty acid biosynthesis. (wikipedia.org)
  • Proteins whose amounts were increased in the mutant were serine hydroxymethyltransferase, β-ketoacyl [acyl carrier protein] synthase II, long-chain fatty acid transport protein, and UDP-glucose 4-epimerase, while the decreased ones were flagellin and D-ribose-binding protein. (elsevierpure.com)
  • Coenzyme A reacts with acyl groups, giving rise to thioester derivatives, such as acetyl-CoA, succinyl-CoA, malonyl-CoA, and 3-hydroxy-3-methylglutaryl (HMG)-CoA. (oregonstate.edu)
  • This enzyme catalyzes the transacylation of malonate from MALONYL CoA to activated holo-ACP, to generate malonyl-(acyl-carrier protein), which is an elongation substrate in FATTY ACIDS biosynthesis. (nih.gov)
  • Universally conserved, the ribosome is a complex ribonucleoparticle that acts as the catalyst for protein synthesis. (encyclopedia.pub)
  • In the mid-1960s, it was revealed that these proteins contain a complex of iron and cysteine sulfur atoms [ 1 ].More data on their electronic and geometric structure was obtained later and it was shown that they are analogs of the active site of proteins. (alliedacademies.org)
  • Two acyl-ACP molecules can be found associated with LYRM proteins as components of complex I in the mitochondrial RC. (nature.com)
  • HN - 2006(1981) BX - Cofilins MH - Actin-Related Protein 2 UI - D051377 MN - D5.750.78.730.246.500 MN - D12.776.220.525.246.500 MS - A PROFILIN binding domain protein that is part of the Arp2-3 complex. (nih.gov)
  • HN - 2006(1998) MH - Actin-Related Protein 2-3 Complex UI - D051376 MN - D5.750.78.730.246 MN - D12.776.220.525.246 MS - A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. (nih.gov)
  • Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments. (nih.gov)
  • HN - 2006 BX - Arp2-3 Complex MH - Actin-Related Protein 3 UI - D051378 MN - D5.750.78.730.246.750 MN - D12.776.220.525.246.750 MS - A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. (nih.gov)
  • 10614) putative ABC transport system permease protein BBZA01000001 CDS ARMA_0010 complement(10625. (go.jp)
  • 11353) putative ABC transport system ATP-binding protein BBZA01000001 CDS ARMA_0011 complement(11350. (go.jp)
  • Also, protein acetylation, like other posttranslational modifications, has been shown to regulate the subcellular localization, the function, and the half-life of many signaling molecules, transcription factors , and enzymes . (oregonstate.edu)
  • MtFAS follows the prokaryotic type II mode where individual enzymatic steps are carried out by separate enzymes (Fig. 1a ) Acyl groups synthesized by mtFAS are linked to 4-phosphopantetheine moiety of the acyl carrier protein (ACP) via a thioester bond. (nature.com)
  • Most acetylated proteins in the body have been modified by the addition of an acetate group that was donated by the coenzyme A thioester derivative, acetyl-CoA. (oregonstate.edu)
  • 9/3/2005) TOTAL DESCRIPTORS = 935 MH - 1-Acylglycerol-3-Phosphate O-Acyltransferase UI - D051103 MN - D8.811.913.50.173 MS - An enzyme that catalyzes the acyl group transfer of ACYL COA to 1-acyl-sn-glycerol 3-phosphate to generate 1,2-diacyl-sn-glycerol 3-phosphate. (nih.gov)
  • Also, the phosphopantetheinyl moiety of coenzyme A is required for the biological activity of several proteins , including the acyl-carrier protein involved in fatty acid synthesis. (oregonstate.edu)
  • Coenzyme A and its acyl derivatives are required for reactions that generate energy from the degradation of dietary fat, carbohydrates , and proteins . (oregonstate.edu)
  • 5181 tryptophan synthase beta chain trpB BBZA01000003 CDS ARMA_0032 5369. (go.jp)
  • PTD forms hydrogen bonds with the components of the membrane through TAT, which has a high density of positive charges and reduces the degree of membrane order through Tryptophan (Trp)-zipper binding to the acyl tails of phospholipid molecules. (bvsalud.org)
  • Aside from commercial value, appropriately designed proteins can also be helpful in the dissection of the physiological function of particular pathways. (nature.com)
  • Ribosomal RNAs (rRNAs) and ribosomal proteins are assembled in a stepwise manner involving a plethora of protein and RNA effectors. (encyclopedia.pub)
  • 23209 ribosomal protein S12 methylthiotransferase rimO BBZA01000003 CDS ARMA_0051 23344. (go.jp)
  • The existence of iron-sulfur cluster proteins was investigated through various sources such as photosynthetic organisms, nitrogen-fixing bacteria, mammalian mitochondrial codons. (alliedacademies.org)
  • Acyl-ACP-LYRM protein complexes facilitate assembly of mitochondrial respiratory complexes. (nature.com)
  • Fatty acids serve a living organism as building blocks of biomembranes, energy storage, cell signaling molecules and ligands in post-translational protein modification. (nature.com)
  • Chetna Dhembla, Ravindra D. Makde, Monica Sundd and Suman Kundu (2021), "Insights into the structure and function of Fatty Acid Pathway Proteins of Leishmania major to identify potent anti-leishmanials", International conference on Infections and Immunity, 8th-10th October 2021, Department of Zoology, Daulat Ram college, University of Delhi, India. (du.ac.in)
  • Especially in the drug discovery field, knowledge of how small molecules associate with proteins plays a fundamental role in understanding how drugs or metabolites can affect cells, tissues, and human metabolism. (uni-freiburg.de)
  • First, the pocket, conservation, structural and energy profiles, and physicochemical properties of (non)covalently bound cysteines were collected from both protein sequences and 3D structures of protein-ligand complexes. (uni-freiburg.de)
  • HN - 2006(1981) BX - Actin-Capping Proteins MH - Actin Depolymerizing Factors UI - D051339 MN - D5.750.78.730.212 MN - D12.776.220.525.212 MS - A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. (nih.gov)
  • The stability and transfer of Fe-S clusters mediated by proteins like NAF-1 regulate cellular resistance to oxidative stress. (alliedacademies.org)
  • AN - coordinate IM with ADENOMA (IM) HN - 2006 BX - Corticotroph Adenoma BX - Pituitary Adenoma, ACTH-Secreting BX - Pituitary Corticotropin-Secreting Adenoma MH - Actin Capping Proteins UI - D051344 MN - D5.750.78.730.32 MN - D12.776.220.525.32 MS - Actin capping proteins are cytoskeletal proteins that bind to the ends of ACTIN FILAMENTS to regulate actin polymerization. (nih.gov)
  • HlyD membrane-fusion protein of T1SS, Barrel-sandwich domain of CusB or HlyD membrane-fusion [Interproscan]. (ntu.edu.sg)
  • Modulating proteins involved in Fe-S cluster transfer represents a potential therapeutic approach for cancer treatment. (alliedacademies.org)
  • The acetylation of histones was found to result in structural changes of the chromatin, which affect both DNA -protein and protein-protein interactions. (oregonstate.edu)
  • Initially in the 1960s, information about them was at the level of a group of unknown proteins that contained iron atoms and had a role in redox functions. (alliedacademies.org)
  • Recombinant Fc-fusion proteins including our variants are superior to those based on H9 superkine in terms of expression levels in mammalian cells, aggregation resistance, stability, in vivo enhancement of immune effector responses, and anti-tumour effect. (bvsalud.org)
  • The NEET family protein NAF-1 plays emerging roles in iron homeostasis, autophagy, cell death pathways, and regulating cellular resistance to oxidative stress. (alliedacademies.org)
  • The result of these findings showed that the active site does not necessarily have to have a protein structure ( Figure 1 ). (alliedacademies.org)
  • However, when expressed as a soluble protein, Ulp1 is toxic to E. coli host cells and most of the protein forms inclusion bodies. (bvsalud.org)
  • 14787 ABC-2 type transport system ATP-binding protein BBZA01000002 CDS ARMA_0027 14784. (go.jp)
  • 41314) energy-coupling factor transport system permease protein ecfT BBZA01000003 CDS ARMA_0064 complement(41356. (go.jp)
  • In this chapter, we explain the widely used GST pull-down assay to identify the protein-protein interaction between purified proteins. (bvsalud.org)
  • It is anticipated that HyperCys will be an effective tool for discovering new potential reactive cysteines in a wide range of nucleophilic proteins and will provide an important contribution to the design of targeted covalent inhibitors with high potency and selectivity. (uni-freiburg.de)
  • Iron-sulfur (Fe-S) clusters are essential protein cofactors involved in various cellular processes. (alliedacademies.org)
  • In this study, we develop a core-shell nanoparticle using a hyaluronic acid (HA)-coated protein transduction domain (PTD) derived from the human immunodeficiency virus (HIV). (bvsalud.org)
  • note = "Funding Information: We are grateful to Drs. Y. Ishii and Y. Itoh (Kyushu University) for assistance with performing two-dimensional gel electrophoresis and protein sequencing, respectively, and M. Ohara for helpful comments. (elsevierpure.com)
  • In cells, RNAs are protected by RNA-binding proteins (RBP) and RNA chaperones and are processed by ribonucleases (RNases). (encyclopedia.pub)