Holo acyl carrier protein fatty carrier proteins. Medical search. Frequent questions

Acyl carrier proteins are critical components of fatty acid and polyketide biosynthesis. (rsc.org)
E.coli lipid A acyltransferases do not have an absolute specificity for 14-carbon hydroxy fatty acids but can transfer fatty acids differing by one carbon unit if the fatty acid substrates are available. (cathdb.info)
When grown on 1% propionic acid, lipid A also contains the odd-chain fatty acids tridecanoic acid, pentadecanoic acid, hydroxytridecanoic acid, and hydroxypentadecanoic acid. (cathdb.info)
In this scenario, hydride is predicted to be added to the re face to generate the S configuration resulting in the same stereochemical outcome as other 3-oxoacyl-ACP reductase (FabG) from bacterial type II fatty acid synthases.These findings are critical advancement for interrogating the biosynthesis of the unusual chemical components of the family of antibiotics of capuramycin. (uky.edu)
The enzyme is capable of transferring the 4'-phosphopantetheine moiety of coenzyme A to a conserved serine residue in both the acyl carrier protein domain of the human cytosolic multifunctional fatty acid synthase and the acyl carrier protein associated independently with human mitochondria. (rhea-db.org)

Cpr37, which belongs to the adenylation domain protein in the nonribosomal peptide synthase (NRPS), subsequently activates PABA and loads it to holo-Cpr36 Two proteins Cpr34 and Cpr35 work in concert to catalyze decarboxylative condensation between a thioester linked PABA and malonyl- S -acyl carrier protein (ACP) during aromatic polyketide biosynthesis catalyzed by type II polyketide synthases. (uky.edu)

Selectivity for the holo over apo -form of acyl carrier proteins is demonstrated indicating that only the phosphopantetheine - thiol is modified. (rsc.org)

We found that sphingomyelin is enriched in migrasomes and identified sphingomyelin synthase 2 (SMS2) as an essential protein for migrasome biogenesis. (bvsalud.org)

Here we present the functional assignment and biochemical characterization of seven proteins, Cpr33-38 and Cpr12, that initiate the biosynthesis of the polyamide. (uky.edu)

The human enzyme, which is expressed mainly in the cytosolic compartment in a wide range of tissues, is a 329-residue, monomeric protein. (rhea-db.org)

Cpr12, encoded by a gene that was originally proposed to be outside the gene cluster and sharing similarity to proteins annotated as ADC lyase, was revealed to catalyze the elimination of pyruvate to form PABA. (uky.edu)

Phylogenetic analysis of the cif protein, which is encoded by the cif gene, shows that the evolution of cifA and cifB is related to each other. (biomedcentral.com)

Cpr36 is demonstrated to function as a free-standingpeptidyl carrier protein (PCP), which is activated to form holo-protein from the apo-form. (uky.edu)

Search proteins in UniProtKB for this molecule. (rhea-db.org)

Small molecules capable of acylating this prosthetic group will provide a simple and reversible means of introducing novel functionality onto carrier protein domains. (rsc.org)
4'-Phosphopantetheinyl transferases (PPTases) play an essential role in activating the carrier protein domains of mega-synthases involved in primary and secondary metabolism and have been validated as promising drug targets in multiple pathogens. (bvsalud.org)

14478 3-oxoacyl-[acyl-carrier protein] reductase fabG BBZA01000001 CDS ARMA_0014 complement(14430. (go.jp)

Also, the phosphopantetheinyl moiety of coenzyme A is required for the biological activity of several proteins , including the acyl-carrier protein involved in fatty acid synthesis. (oregonstate.edu)
Mitochondrial fatty acid synthesis (mtFAS) is essential for respiratory function. (nature.com)
MtFAS generates the octanoic acid precursor for lipoic acid synthesis, but the role of longer fatty acid products has remained unclear. (nature.com)
Recently the mitochondrial fatty acid synthesis pathway (mtFAS) has been identified as a mechanism to regulate mitochondrial respiratory chain (RC) function to meet the substrate availability (acetyl-CoA) to tricarboxylic acid cycle 4 , 5 . (nature.com)
Fig. 1: Schematic representation of the mitochondrial fatty acid synthesis (mtFAS) pathway, wild-type and engineered MECR and the reaction catalyzed by MECR/Etr1. (nature.com)

This enzyme catalyzes the transacylation of malonate from MALONYL CoA to activated holo-ACP, to generate malonyl-(acyl-carrier protein), which is an elongation substrate in FATTY ACIDS biosynthesis. (nih.gov)
It is an essential enzyme in the biosynthesis of FATTY ACIDS in all BACTERIA . (nih.gov)
Coenzyme A and its derivatives inhibit the synthesis of 4'-phosphopantothenic acid, but the inhibition can be reversed by carnitine , required for the transport of fatty acids into the mitochondria (3) . (oregonstate.edu)
Our in vitro and in vivo analyses indicate that the MECR G165Q variant allows synthesis of octanoyl groups but not long chain fatty acids, confirming the validity of our computational approach to engineer substrate length specificity. (nature.com)
Fatty acids serve a living organism as building blocks of biomembranes, energy storage, cell signaling molecules and ligands in post-translational protein modification. (nature.com)
MtFAS is capable of generating fatty acids longer than eight carbons, but the precise function of these longer fatty acids has remained unclear. (nature.com)

Coenzyme A reacts with acyl groups, giving rise to thioester derivatives, such as acetyl-CoA, succinyl-CoA, malonyl-CoA, and 3-hydroxy-3-methylglutaryl (HMG)-CoA. (oregonstate.edu)
Most acetylated proteins in the body have been modified by the addition of an acetate group that was donated by the coenzyme A thioester derivative, acetyl-CoA. (oregonstate.edu)
MtFAS follows the prokaryotic type II mode where individual enzymatic steps are carried out by separate enzymes (Fig. 1a ) Acyl groups synthesized by mtFAS are linked to 4-phosphopantetheine moiety of the acyl carrier protein (ACP) via a thioester bond. (nature.com)

In addition, coenzyme A in the form of acetyl-CoA and succinyl-CoA is involved in the citric acid cycle , in the synthesis of essential fats, cholesterol , steroid hormones , vitamins A and D, the neurotransmitter acetylcholine, and in the fatty acid β- oxidation pathway. (oregonstate.edu)
As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins. (elsevierpure.com)

We identified proteins whose amounts were altered in a temperature-sensitive dnaA46 mutant of Escherichia coli. (elsevierpure.com)
protein_coding" "AAC73502","phoB","Escherichia coli","response regulator in two-component regulatory system with PhoR [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC73720","dpiB","Escherichia coli","sensory histidine kinase in two-component regulatory system with CitB [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC73789","kdpD","Escherichia coli","fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74078","torS","Escherichia coli","hybrid sensory histidine kinase in two-component regulatory system with TorR [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74079","torT","Escherichia coli","periplasmic sensory protein associated with the TorRS two-component regulatory system [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74080","torR","Escherichia coli","response regulator in two-component regulatory system with TorS [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74155","flgM","Escherichia coli","anti-sigma factor for FliA (sigma 28) [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74158","flgC","Escherichia coli","flagellar component of cell-proximal portion of basal-body rod [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74163","flgH","Escherichia coli","flagellar protein of basal-body outer-membrane L ring [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC73262","fhuC","Escherichia coli","iron(3+)-hydroxamate import ABC transporter ATPase [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC73263","fhuD","Escherichia coli","iron(3+)-hydroxamate import ABC transporter periplasmic binding protein [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC73829","ybgE","Escherichia coli","putative inner membrane protein in cydABX-ybgE operon [Ensembl]. (ntu.edu.sg)
protein_coding" "AAC74417","ogt","Escherichia coli","O-6-alkylguanine-DNA:cysteine-protein methyltransferase [Ensembl]. (ntu.edu.sg)

Coenzyme A and its acyl derivatives are required for reactions that generate energy from the degradation of dietary fat, carbohydrates , and proteins . (oregonstate.edu)
9/3/2005) TOTAL DESCRIPTORS = 935 MH - 1-Acylglycerol-3-Phosphate O-Acyltransferase UI - D051103 MN - D8.811.913.50.173 MS - An enzyme that catalyzes the acyl group transfer of ACYL COA to 1-acyl-sn-glycerol 3-phosphate to generate 1,2-diacyl-sn-glycerol 3-phosphate. (nih.gov)

Also, protein acetylation, like other posttranslational modifications, has been shown to regulate the subcellular localization, the function, and the half-life of many signaling molecules, transcription factors , and enzymes . (oregonstate.edu)
PTD forms hydrogen bonds with the components of the membrane through TAT, which has a high density of positive charges and reduces the degree of membrane order through Tryptophan (Trp)-zipper binding to the acyl tails of phospholipid molecules. (bvsalud.org)
Two acyl-ACP molecules can be found associated with LYRM proteins as components of complex I in the mitochondrial RC. (nature.com)
Especially in the drug discovery field, knowledge of how small molecules associate with proteins plays a fundamental role in understanding how drugs or metabolites can affect cells, tissues, and human metabolism. (uni-freiburg.de)

Recombinant Fc-fusion proteins including our variants are superior to those based on H9 superkine in terms of expression levels in mammalian cells, aggregation resistance, stability, in vivo enhancement of immune effector responses, and anti-tumour effect. (bvsalud.org)
High yield purification of Ulp1 is required during the isolation and purification of SUMO-tagged recombinant proteins. (bvsalud.org)
Structural and functional characterization of recombinant human haemoglobin as an artificial oxygen carrier. (du.ac.in)

Glutathione-s-transferase (GST) protein is a widely used affinity tag for affinity purification. (bvsalud.org)

The existence of iron-sulfur cluster proteins was investigated through various sources such as photosynthetic organisms, nitrogen-fixing bacteria, mammalian mitochondrial codons. (alliedacademies.org)

Periplasmic binding proteins and sugar binding domain of LacI family [Interproscan]. (ntu.edu.sg)
Periplasmic binding protein [Interproscan]. (ntu.edu.sg)
Copper binding periplasmic protein CusF [Interproscan]. (ntu.edu.sg)

Acyl-ACPs form complexes with adapter proteins that contain a leucine-tyrosine-arginine motif (LYRM) 6 . (nature.com)
Acyl-ACP-LYRM protein complexes facilitate assembly of mitochondrial respiratory complexes. (nature.com)
First, the pocket, conservation, structural and energy profiles, and physicochemical properties of (non)covalently bound cysteines were collected from both protein sequences and 3D structures of protein-ligand complexes. (uni-freiburg.de)

AN - coordinate IM with ADENOMA (IM) HN - 2006 BX - Corticotroph Adenoma BX - Pituitary Adenoma, ACTH-Secreting BX - Pituitary Corticotropin-Secreting Adenoma MH - Actin Capping Proteins UI - D051344 MN - D5.750.78.730.32 MN - D12.776.220.525.32 MS - Actin capping proteins are cytoskeletal proteins that bind to the ends of ACTIN FILAMENTS to regulate actin polymerization. (nih.gov)
The stability and transfer of Fe-S clusters mediated by proteins like NAF-1 regulate cellular resistance to oxidative stress. (alliedacademies.org)
CRP appears to interact with the Sxy protein to regulate some genes related to DNA uptake (natural competence). (unam.mx)

Chetna Dhembla, Ravindra D. Makde, Monica Sundd and Suman Kundu (2021), "Insights into the structure and function of Fatty Acid Pathway Proteins of Leishmania major to identify potent anti-leishmanials", International conference on Infections and Immunity, 8th-10th October 2021, Department of Zoology, Daulat Ram college, University of Delhi, India. (du.ac.in)

Cyd operon protein YbgE (Cyd_oper_YbgE) [Interproscan]. (ntu.edu.sg)

chlorate resistance protein [Ensembl]. (ntu.edu.sg)

10614) putative ABC transport system permease protein BBZA01000001 CDS ARMA_0010 complement(10625. (go.jp)
41314) energy-coupling factor transport system permease protein ecfT BBZA01000003 CDS ARMA_0064 complement(41356. (go.jp)

Binding-protein-dependent transport system inner membrane component [Interproscan]. (ntu.edu.sg)

11353) putative ABC transport system ATP-binding protein BBZA01000001 CDS ARMA_0011 complement(11350. (go.jp)

Sigma-54 interaction domain, Bacterial regulatory protein, Propionate catabolism activator [Interproscan]. (ntu.edu.sg)
Transcriptional regulatory protein, Response regulator receiver domain [Interproscan]. (ntu.edu.sg)
Bacterial regulatory proteins, Response regulator receiver domain [Interproscan]. (ntu.edu.sg)
Protein of unknown function (DUF1439) [Interproscan]. (ntu.edu.sg)
FlgN protein [Interproscan]. (ntu.edu.sg)
Flagella basal body rod protein, Flagellar basal body rod FlgEFG protein C-terminal [Interproscan]. (ntu.edu.sg)
FlgD Ig-like domain, FlgD Tudor-like domain, Flagellar hook capping protein - N-terminal region [Interproscan]. (ntu.edu.sg)
Flagellar L-ring protein [Interproscan]. (ntu.edu.sg)
Rod binding protein, Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase [Interproscan]. (ntu.edu.sg)
Peroxide stress protein YaaA [Interproscan]. (ntu.edu.sg)
HlyD membrane-fusion protein of T1SS, Barrel-sandwich domain of CusB or HlyD membrane-fusion [Interproscan]. (ntu.edu.sg)
YdfZ protein [Interproscan]. (ntu.edu.sg)

HN - 2006(1981) BX - Cofilins MH - Actin-Related Protein 2 UI - D051377 MN - D5.750.78.730.246.500 MN - D12.776.220.525.246.500 MS - A PROFILIN binding domain protein that is part of the Arp2-3 complex. (nih.gov)
HN - 2006(1998) MH - Actin-Related Protein 2-3 Complex UI - D051376 MN - D5.750.78.730.246 MN - D12.776.220.525.246 MS - A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. (nih.gov)
Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments. (nih.gov)
HN - 2006 BX - Arp2-3 Complex MH - Actin-Related Protein 3 UI - D051378 MN - D5.750.78.730.246.750 MN - D12.776.220.525.246.750 MS - A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. (nih.gov)
In the mid-1960s, it was revealed that these proteins contain a complex of iron and cysteine sulfur atoms [ 1 ].More data on their electronic and geometric structure was obtained later and it was shown that they are analogs of the active site of proteins. (alliedacademies.org)

The NEET family protein NAF-1 plays emerging roles in iron homeostasis, autophagy, cell death pathways, and regulating cellular resistance to oxidative stress. (alliedacademies.org)

Pull-down assay is a technique to analyze direct protein-protein interaction under in vitro condition. (bvsalud.org)
Also, this technique is appropriate for investigating the direct interaction between two purified proteins. (bvsalud.org)
In this chapter, we explain the widely used GST pull-down assay to identify the protein-protein interaction between purified proteins. (bvsalud.org)

Iron-Sulfur (Fe-S) clusters are ancient, versatile protein cofactors that are critical for various cellular processes including electron transfer, regulation of gene expression, and metabolism. (alliedacademies.org)

HN - 2006(1981) BX - Actin-Capping Proteins MH - Actin Depolymerizing Factors UI - D051339 MN - D5.750.78.730.212 MN - D12.776.220.525.212 MS - A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. (nih.gov)

Protein acetylation alters the overall charge of proteins, modifying their three-dimensional structure and, potentially, their function. (oregonstate.edu)
Furthermore, our data imply that restoring lipoylation in mtFAS deficient yeast strains is not sufficient to support respiration and that long chain acyl-ACPs generated by mtFAS are required for mitochondrial function. (nature.com)
Aside from commercial value, appropriately designed proteins can also be helpful in the dissection of the physiological function of particular pathways. (nature.com)
We are interested in studying the function of these long-chain acyl groups synthesized by mtFAS and associated with ACPs. (nature.com)

Initially in the 1960s, information about them was at the level of a group of unknown proteins that contained iron atoms and had a role in redox functions. (alliedacademies.org)

However, when expressed as a soluble protein, Ulp1 is toxic to E. coli host cells and most of the protein forms inclusion bodies. (bvsalud.org)

Iron-sulfur (Fe-S) clusters are essential protein cofactors involved in various cellular processes. (alliedacademies.org)

14787 ABC-2 type transport system ATP-binding protein BBZA01000002 CDS ARMA_0027 14784. (go.jp)

The acetylation of histones was found to result in structural changes of the chromatin, which affect both DNA -protein and protein-protein interactions. (oregonstate.edu)

The result of these findings showed that the active site does not necessarily have to have a protein structure ( Figure 1 ). (alliedacademies.org)

In this study, we develop a core-shell nanoparticle using a hyaluronic acid (HA)-coated protein transduction domain (PTD) derived from the human immunodeficiency virus (HIV). (bvsalud.org)

note = "Funding Information: We are grateful to Drs. Y. Ishii and Y. Itoh (Kyushu University) for assistance with performing two-dimensional gel electrophoresis and protein sequencing, respectively, and M. Ohara for helpful comments. (elsevierpure.com)

Modulating proteins involved in Fe-S cluster transfer represents a potential therapeutic approach for cancer treatment. (alliedacademies.org)
It is anticipated that HyperCys will be an effective tool for discovering new potential reactive cysteines in a wide range of nucleophilic proteins and will provide an important contribution to the design of targeted covalent inhibitors with high potency and selectivity. (uni-freiburg.de)

Anatomy2

Organisms10

Diseases1

Chemicals and Drugs62

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes22

Technology, Industry, Agriculture1

Information Science3

Health Care1

Acid5

Polyketide biosynthesis1

Selectivity1

Synthase1

Biosynthesis1

Enzyme1

Lyase1

Gene1

Form1

Search1

Domains2

Reductase1

Acid synthesis5

ACIDS6

Thioester3

Synthesis2

Escherichia14

Generate2

Molecules4

Recombinant3

Transferase1

Iron-sulfur1

Periplasmic3

Complexes3

Regulate3

Pathway1

Operon1

Ensembl1

Permease2

Inner membrane1

Putative1

Interproscan12

Complex5

Resistance1

Interaction3

Including electron1

Family1

Function4

Role1

Coli1

Essential1

Type1

Found1

Structure1

Domain1

Helpful1

Potential2