• In enzymology and molecular biology, a holo-[acyl-carrier-protein] synthase (ACPS, EC 2.7.8.7) is an enzyme that catalyzes the chemical reaction: CoA-[4'-phosphopantetheine] + apo-acyl carrier protein ⇌ {\displaystyle \rightleftharpoons } adenosine 3',5'-bisphosphate + holo-acyl carrier protein This enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. (wikipedia.org)
  • All ACPS enzymes known so far are evolutionally related to each other in a single superfamily of proteins. (wikipedia.org)
  • This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP. This superfamily consists of two subtypes: the trimeric ACPS type such as E. coli ACPS and the monomeric Sfp (PCP-synthesizing) type such as B. subtilis SFP. (wikipedia.org)
  • Other names in common use, disregarding the synthetase/synthase spelling difference, include acyl carrier protein holoprotein synthetase, holo-ACP synthetase, coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine, acyl carrier protein synthetase (ACPS), PPTase, acyl carrier protein synthase, P-pant transferase, and CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase. (wikipedia.org)
  • citrate lyase holo-[acyl-carrier protein] synthase (EC 2.7.7.61). (lbl.gov)
  • Catalyzes the formation of 2-(5''-triphosphoribosyl)-3'- dephosphocoenzyme-A, the precursor of the prosthetic group of the holo- acyl carrier protein (gamma chain) of citrate lyase, from ATP and dephospho-CoA. (string-db.org)
  • Transfers 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A on a serine residue to the apo-acyl carrier protein (gamma chain) of the citrate lyase to yield holo-acyl carrier protein. (string-db.org)
  • The systematic name of this enzyme class is CoA-[4'-phosphopantetheine]:apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase. (wikipedia.org)
  • UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (EC 2.3.1.191, UDP-3-O-acyl-glucosamine N-acyltransferase, UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase, acyltransferase LpxD, acyl-ACP:UDP-3-O-(3-hydroxyacyl)-GlcN N-acyltransferase, firA (gene), lpxD (gene)) is an enzyme with systematic name (3R)-3-hydroxymyristoyl-(acyl-carrier protein):UDP-3-O-((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine N-acetyltransferase. (wikipedia.org)
  • GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)
  • Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
  • The human enzyme, which is expressed mainly in the cytosolic compartment in a wide range of tissues, is a 329-residue, monomeric protein. (rhea-db.org)
  • The enzyme is capable of transferring the 4'-phosphopantetheine moiety of coenzyme A to a conserved serine residue in both the acyl carrier protein domain of the human cytosolic multifunctional fatty acid synthase and the acyl carrier protein associated independently with human mitochondria. (rhea-db.org)
  • Cpr12, encoded by a gene that was originally proposed to be outside the gene cluster and sharing similarity to proteins annotated as ADC lyase, was revealed to catalyze the elimination of pyruvate to form PABA. (uky.edu)
  • LpxD from Escherichia coli prefers (R,S)-3-hydroxymyristoyl-[acyl- carrier protein] over (R,S)-3-hydroxypalmitoyl-[acyl-carrier protein]. (cathdb.info)
  • The codon table used was the FreqB table from " Design Parameters to Control Synthetic Gene Expression in Escherichia coli ", which was used because it was shown to express proteins well and was recommended to us by JCVI. (freegenes.org)
  • The human 4'-phosphopantetheine transferase is also capable of phosphopantetheinylation of peptidyl carrier and acyl carrier proteins from prokaryotes. (rhea-db.org)
  • Selectivity for the holo over apo -form of acyl carrier proteins is demonstrated indicating that only the phosphopantetheine - thiol is modified. (rsc.org)
  • It transfers a 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to an invariant serine in an acyl carrier protein (ACP), a small protein responsible for acyl group activation in fatty acid biosynthesis. (wikipedia.org)
  • Lipoate protein ligase B primarily recognizes the C8-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein. (du.ac.in)
  • Acyl carrier proteins are critical components of fatty acid and polyketide biosynthesis. (rsc.org)
  • Cpr37, which belongs to the adenylation domain protein in the nonribosomal peptide synthase (NRPS), subsequently activates PABA and loads it to holo-Cpr36 Two proteins Cpr34 and Cpr35 work in concert to catalyze decarboxylative condensation between a thioester linked PABA and malonyl- S -acyl carrier protein (ACP) during aromatic polyketide biosynthesis catalyzed by type II polyketide synthases. (uky.edu)
  • Structures of the protein-chromophore complex and the apoprotein form of neocarzinostatin were determined at 1.8 angstrom resolution. (caltech.edu)
  • Here we present the functional assignment and biochemical characterization of seven proteins, Cpr33-38 and Cpr12, that initiate the biosynthesis of the polyamide. (uky.edu)
  • Both the precursor and mature forms are substrates for phosphopantetheine attachment by a soluble chloroplast holo-acyl carrier protein synthase. (uchicago.edu)
  • Acyl carrier protein (ACP) import into chloroplasts does not require the phosphopantetheine: evidence for a chloroplast holo-ACP synthase. (uchicago.edu)
  • This island contains ribosomal proteins or RNA related elements and may indicate a False Positive Prediction! (up.ac.za)
  • acyl-[SfmA_non-ribosomal_peptide_synthase] = 2 reactions were found. (brenda-enzymes.org)
  • Functional reintegration into lipid environments represents a major challenge for in vitro investigation of integral membrane proteins (IMPs). (tudelft.nl)
  • There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Bacillus subtilis genome using these genes and the sgRNAs available from the Bacillus Genetic Stock Center. (freegenes.org)
  • GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)
  • Phylogenetic analysis of the cif protein, which is encoded by the cif gene, shows that the evolution of cifA and cifB is related to each other. (biomedcentral.com)
  • In 1992, Saito and co-workers showed that the reaction of holo-NCS with small thiols, such as β-mercaptoethanol (BME), takes a different course, to form a product that is formally a 1:1:1 adduct of thiol, 1, and water. (caltech.edu)
  • Conserved hypothetical protein [Ensembl]. (ntu.edu.sg)
  • Conserved hypothetical protein, UPF0052 family. (ntu.edu.sg)
  • hypothetical protein [Ensembl]. (ntu.edu.sg)
  • Activation of the acyl carrier protein (ACP) domain of the SwfA protein to its holo-form by co-expression with Svp is the first functional proof of swf type genes in marine sponges. (cirn-na.com)
  • membrane associated protein [Ensembl]. (ntu.edu.sg)
  • Dihydromonacolin L acid is synthesized while bound to an acyl-carrier protein domain of the lovastatin nonaketide synthase (EC 2.3.1.161 ). (genome.jp)
  • The chromophore displays marked nonlinearity of the triple bonds and is bound noncovalently in a pocket formed by the two protein domains. (caltech.edu)
  • Their ability to function as potent inhibitors of oligosaccharide synthesis in eukaryotic cells has established them as unique biochemical probes of the role of glycosylation on protein structure and function. (caltech.edu)
  • Uncharacterised protein family UPF0052 [Interproscan]. (ntu.edu.sg)
  • UPF0178 family protein. (string-db.org)
  • The tunicamycins are a family of natural products represented generally by structure 1, wherein R indicates one of several long-chain branched, linear, saturated or unsaturated acyl substituents. (caltech.edu)
  • Ras proteins have been implicated to function as components of the cellular signal transduction pathways related to cell proliferation and differentiation. (caltech.edu)
  • Search proteins in UniProtKB for this molecule. (rhea-db.org)
  • In their oncogenic form, the natural GTP-ase activity of Ras proteins is inhibited, leading to overstimulation of the signaling pathway for cell growth. (caltech.edu)
  • Neocarzinostatin is composed of a labile chromophore with DNA-cleaving activity and a stabilizing protein. (caltech.edu)
  • Cpr36 is demonstrated to function as a free-standingpeptidyl carrier protein (PCP), which is activated to form holo-protein from the apo-form. (uky.edu)
  • Small molecules capable of acylating this prosthetic group will provide a simple and reversible means of introducing novel functionality onto carrier protein domains. (rsc.org)
  • 4'-Phosphopantetheinyl transferases (PPTases) play an essential role in activating the carrier protein domains of mega-synthases involved in primary and secondary metabolism and have been validated as promising drug targets in multiple pathogens. (bvsalud.org)
  • 3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-3-O-((3R)- hydroxytetradecanoyl)-alpha-D-glucosamine = UDP-2-N,3-O-bis((3R)-3- hydroxytetradecanoyl)-alpha-D-glucosamine + a holo-[acyl-carrier- protein]. (cathdb.info)
  • The five-membered pyrrolidine ring has an envelope conformation and the silacyclopentyl ring has an en-type conformation with C17 and C18 on opposite sides of the C16-Si-C19 plane. (caltech.edu)