The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). (nih.gov)
The hnRNP proteins have distinct nucleic acid binding properties. (nih.gov)
These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks and hyperdynamic binding of structural chromatin proteins. (biomedcentral.com)
Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose. (fishersci.com)
The protein produced from this gene, called heterogenous nuclear ribonucleoprotein K (hnRNP K), attaches (binds) to DNA or its chemical cousin RNA and to other proteins, acting as a docking station for these molecules so they can interact. (medlineplus.gov)
By bringing certain proteins together with DNA or RNA, the hnRNP K protein helps control the activity of genes and the production of proteins. (medlineplus.gov)
By regulating certain genes and proteins, the hnRNP K protein plays a role in the normal development and function of several body systems, including the brain. (medlineplus.gov)
These events regulate the inclusion of repetitive GY-SLiMs within nearly all members of the heterogeneous nuclear ribonucleoprotein (hnRNP) A and D families of RNA binding proteins. (cam.ac.uk)
There were no polypeptides in the isolated antigen which corresponded in MW to the core proteins of heterogeneous nuclear ribonucleoprotein (hnRNP) particles described by other investigators. (eurekamag.com)
We have determined the effect of seven serine- and arginine-rich (SR) proteins and 15 heterogenous nuclear ribonucleoproteins (hnRNPs) on human papillomavirus type 16 (HPV16) late gene expression. (lu.se)
Of the 15 hnRNP proteins analyzed here, hnRNP A2, hnRNP F, and hnRNP H efficiently induced HPV16 late gene expression, and all of the hnRNPs affected HPV16 L1 mRNA levels or mRNA splicing. (lu.se)
It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. (antibodies-online.com)
This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. (antibodies-online.com)
A family of RNA-BINDING PROTEINS that contain an RNA RECOGNITION MOTIF and two ribonucleoprotein (RNP) domains which bind RNA , in addition to other domains that allow for high affinity binding, sequence specificity, and protein interactions. (nih.gov)
Nab3 and Nrd1 are yeast heterogeneous nuclear ribonucleoprotein (hnRNP)-like proteins that heterodimerize and bind RNA. (chksignal.com)
The self-association property of Nab3 adds to the previously documented interactions between these hnRNP-like proteins, RNA polymerase II, and the nascent transcript, Selleck Entinostat leading to a network of nucleoprotein interactions that define a higher order Nrd1-Nab3 complex. (chksignal.com)
The heterogeneous nuclear ribonucleoprotein (hnRNP) C proteins, among the most abundant pre-mRNA-binding proteins in the eukaryotic nucleus, have a single RNP motif RNA-binding domain. (korea.ac.kr)
Protein12
Heterogeneous nuclear ribonucleoprotein A3 is a protein that in humans is encoded by the HNRNPA3 gene. (wikipedia.org)
Purification and partial sequencing of the nuclear autoantigen RA33 shows that it is indistinguishable from the A2 protein of the heterogeneous nuclear ribonucleoprotein complex. (jci.org)
Sequence analysis of five tryptic peptides revealed that their sequences matched corresponding sequences of the A2 protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. (jci.org)
HNRNPK gene mutations that cause Au-Kline syndrome result in the production of little or no hnRNP K protein. (medlineplus.gov)
The differential inclusion of these exons impacts the formation of hnRNP-driven higher-order protein assemblies required for the regulation of target AS events. (cam.ac.uk)
A maximum of 2.9% of the nuclear RNA and 0.7% of the protein bound to anti-nRNP but not to control IgG columns. (eurekamag.com)
A fraction of the bound antigen, comprising less than 0.15% of the total nuclear protein, was isolated in antigenically active form. (eurekamag.com)
The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. (antibodies-online.com)
Splicing of the NS segment, which encodes the host shutoff protein non-structural protein 1 (NS1), generates the nuclear export protein (NEP/NS2) that is involved in viral RNP export from the host nucleus [ 8 ]. (biomedcentral.com)
Cells stressed by osmotic shock (OSM) activate the mitogen-activated protein kinase kinase(3/6)-p38 signaling pathway, which in turn results in accumulation of hnRNP A1 in the cytoplasm. (pasteur.fr)
This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. (sitoolsbiotech.com)
[ 2 , 4 ] Protein sequencing of highly purified RA33 revealed that it is indistinguishable from heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2B1). (medscape.com)
Anti-hnRNP L antibody1
Use Anti-hnRNP L Antibody, clone 4D11 (Mouse Monoclonal Antibody) validated in western blot, immunoprecipitation to detect hnRNP L also known as heterogeneous nuclear ribonucleoprotein L. (fishersci.com)
The following product was used in this experiment: hnRNP-K Polyclonal Antibody from Thermo Fisher Scientific, catalog # IHC-00088. (thermofisher.com)
Binds2
Antioxidant genes such as ferritin are transcriptionally activated in oxidative stress via the antioxidant responsive element (ARE), to which nuclear factor-E2-related factor 2 (Nrf2) binds and activates transcription. (embl.de)
The identified mutants were further tested for binding to poly(U)-Sepharose, a substrate to which wild type hnRNP C1 binds with high affinity. (korea.ac.kr)
HnRNPs1
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). (nih.gov)
Antigen1
To characterize RA33, the antigen was purified from HeLa cell nuclear extracts to more than 90% homogeneity by affinity chromatography on heparin-Sepharose and by chromatofocusing. (jci.org)
RA333
RA33 is a nuclear autoantigen with an apparent molecular mass of 33 kD. (jci.org)
Furthermore, RA33 was shown to be present in the 40S hnRNP complex and to behave indistinguishably from A2 in binding to single stranded DNA. (jci.org)
RA33 is a nuclear autoantigen with a molecular weight of approximately 33 kDa. (medscape.com)
Motif1
This treatment results in serine-specific phosphorylation within a C-terminal peptide, dubbed the "F-peptide," which is adjacent to the M9 motif that mediates bidirectional transport of hnRNP A1. (pasteur.fr)
Molecule1
https://medicalxpress.com/news/2018-09-molecule-anti-aging-effects-vascular.html β-Hydroxybutyrate Prevents Vascular Senescence through hnRNP A1-Mediated Upregulation of Oct4. (crsociety.org)
Genes1
Arsenic exposure of human HaCaT keratinocytes induced nuclear accumulation of PRMT1 and PRMT4, histone H4R3 and H3R17 methylation proximal to the ARE, but not to the non-ARE regions of ferritin genes. (embl.de)
Tumor5
hnRNP G elicits tumor-suppressive activity in part by upregulating the expression of Txnip. (uclahealth.org)
Heterogeneous nuclear ribonucleoprotein G (hnRNP G) demonstrates tumor suppressive effect against oral squamous cell carcinoma cells. (uclahealth.org)
The recent developments of nuclear medicine in oncology have involved numerous investigations GS-9973 solubility dmso of novel specific tumor-targeting radiopharmaceuticals as a major area of interest for both cancer imaging and therapy. (chksignal.com)
Grgurević, Lovorka (2019) Tumor tissue hnRNP M and HSP 90α as potential predictors of disease-specific mortality in patients with early-stage cutaneous head and neck melanoma: a proteomics-based study . (irb.hr)
Conclusions and Significance: Data suggest that tumor tissue expression of hnRNP M and/or of HSP 90α deserve further investigation and clinical validation as potential novel risk stratification aids in patients with stage I-II cutaneous head and neck malignant melanoma. (irb.hr)
Cytoplasm1
The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. (sitoolsbiotech.com)
Phosphorylation5
This study therefore identifies a cancer-specific mechanism of hnRNP L phosphorylation and subsequent lowering of the caspase-9a/9b ratio, which is required for the tumorigenic capacity of NSCLC cells. (elsevierpure.com)
This effect modulates alternative splicing regulation in vivo and correlates with increased hnRNP A1 phosphorylation. (pasteur.fr)
Analysis of mutants in which the F-peptide serines were replaced by aspartic acids or alanines showed that F-peptide phosphorylation is required for the subcellular redistribution of hnRNP A1 in cells subjected to OSM. (pasteur.fr)
Furthermore, F-peptide phosphorylation modulates the interaction of hnRNP A1 with transportin Trn1. (pasteur.fr)
Our findings suggest that the phosphorylation of F-peptide by cell-signaling pathways regulates the rate of hnRNP A1 nuclear import. (pasteur.fr)
Characterize1
The feasibility of using human autoimmune antibodies to isolate and characterize specific nuclear ribonucleoprotein (nRNP) complexes was investigated. (eurekamag.com)
Identification1
We have devised a phage display-based screening method for the rapid identification of amino acids in hnRNP C1 that are essential for its binding to RNA. (korea.ac.kr)
MRNA2
Importantly, in a mouse xenograft model, downregulation of hnRNP L in NSCLC cells induced a complete loss of tumorigenic capacity that was due to the changes in caspase-9 pre-mRNA processing. (elsevierpure.com)
β-HB binding to hnRNP A1 markedly enhances hnRNP A1 binding with Octamer-binding transcriptional factor (Oct) 4 mRNA, which stabilizes Oct4 mRNA and Oct4 expression. (crsociety.org)
Polymerase1
Each segment is wrapped by a nucleoprotein (NP) into a viral ribonucleoprotein (RNP) complex along with a viral RNA polymerase complex comprising polymerase basic 1 (PB1) polymerase, cap‐binding PB2 subunit, and polymerase acidic (PA) endonuclease. (biomedcentral.com)
Vivo1
The in vivo produced transcripts are heterogeneous (due to any of promoter? (iresite.org)
Accumulation1
We have characterized the molecular mechanism involved in the cytoplasmic accumulation of hnRNP A1 in NIH 3T3 cells subjected to OSM. (pasteur.fr)
5FMC is a nuclear complex that can only be recruited by Chtop when the latter is arginine-methylated by Prmt1. (embl.de)
Expression3
p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G. DNA Repair. (uclahealth.org)
Heterogeneous nuclear ribonucleoprotein L (hnRNP L) interacted with this ESS, and downregulation of hnRNP L expression induced an increase in the caspase-9a/9b ratio. (elsevierpure.com)
Although expression of hnRNP L lowered the caspase-9a/9b ratio in NSCLC cells, expression of hnRNP L produced the opposite effect in non-transformed cells, suggesting a post-translational modification specific for NSCLC cells. (elsevierpure.com)
Splicing factor2
encodes an SC35-like splicing factor of 28 kD localized to the nuclear specks. (or.jp)
Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an alternative splicing factor that is mainly nuclear, although it shuttles rapidly between nuclear and cytoplasmic compartments. (pasteur.fr)
Cells1
We conclude that β-HB exerts anti-aging effects in vascular cells by upregulating an hnRNP A1-induced Oct4-mediated Lamin B1 pathway. (crsociety.org)
Domain1
Its M9 domain acts as both a nuclear localization and nuclear export signal. (antibodies-online.com)