- Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
- This monoclonal antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. (novusbio.com)
- The following product was used in this experiment: HLA-DR Monoclonal Antibody (LN3), Super Bright™ 436, eBioscience™ from Thermo Fisher Scientific, catalog # 62-9956-42, RRID AB_2744822. (thermofisher.com)
- HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes. (thermofisher.com)
- The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response. (thermofisher.com)
- Methods Homocitrullinated peptides were identified and assessed in vitro for HLA-A2 binding and in vivo in human leukocyte antigen (HLA) transgenic mouse models for immunogenicity. (bmj.com)
- Modified peptides showed enhanced binding to HLA-A2 compared with the native sequences and immunization of HLA-A2 transgenic mice generated high avidity modification specific CD8 responses that killed peptide expressing target cells. (bmj.com)
- Recent observations raise the hypothesis that not only the drug/chemical, but also parts of the haptenated protein or peptides may constitute the important structural determinants for antigen recognition by the TCR. (frontiersin.org)
- Pathogenic human thyroglobulin peptides in HLA-DR3 transgenic mouse model of autoimmune thyroiditis. (musc.edu)
- Peptides are a promising class of gelators, due to their structural simplicity, biocompatibility and versatility. (bvsalud.org)
- Exposure of endothelial cells to anti-b2GPI antibodies and their corresponding peptides leads to the inhibition of endothelial cell activation, as shown by decreased expression of the adhesion molecules E-selectin, intercellular adhesion molecule, and vascular cell adhesion molecule and of monocyte adhesion. (medscape.com)
- In vivo infusion of each of the anti-b2GPI antibodies into BALB/c mice followed by administration of the corresponding specific peptides prevents the peptide-treated mice from developing experimental APS. (medscape.com)
- The allelic variations among different HLA molecules are a major factor accounting for differences in the types of antigenic peptides to which an individual responds or in the types of T cells that are used in an immune response. (musculoskeletalkey.com)
- Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
- Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
- An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*03 alleles. (musc.edu)
- The polymorphisms associated with the "shared epitope" are located on the α-helical rim (DRB1 chain) of the peptide-binding cleft, where they may interact with either the bound peptide antigen or the T cell receptor. (musculoskeletalkey.com)
- The association between aPL antibodies and particular human leukocyte antigen (HLA) alleles and HLA-linked epitopes has been reported in studies of patients with lupus erythematous (eg, HLA-DR7, HLA-DR4). (medscape.com)
- The HLA-DR3 phenotypes seem to predispose to the formation of aCL antibodies and antinuclear antibodies (ANAs), but this has not been confirmed in patients, and particular HLA alleles associated with recurrent miscarriage have not been reported. (medscape.com)
- In the case of systemic lupus erythematosus (SLE) and related illnesses, many of the HLA class II alleles are associated with the presence of specific autoantibodies or clinical phenotypes. (musculoskeletalkey.com)
- Another useful measure of LD is the correlation coefficient, r 2 , between alleles at A and B. Like D′, the value of r 2 can also vary between 0 and 1. (musculoskeletalkey.com)
- In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. (novusbio.com)
- Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. (thermofisher.com)
- Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. (thermofisher.com)
- C-l-LL and C-l-YY, which are among the smallest peptide LMOGs reported to date, are robust gelators with a large radius of gelation (13.44 MPa1/2 and 13.90 MPa1/2, respectively), and even outperformed l-FF (5.61 MPa1/2). (bvsalud.org)
- HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). (thermofisher.com)
- Coexpression of susceptible and resistant HLA class II transgenes in murine experimental autoimmune thyroiditis: DQ8 molecules downregulate DR3-mediated thyroiditis. (musc.edu)
- The HLA molecules and their counterparts in rodents were subsequently shown to be directly responsible for immune response differences between individuals and for determining the likelihood of graft rejection. (musculoskeletalkey.com)
- In each individual, T cells are generally restricted to recognize antigens presented by the person's own HLA molecules. (musculoskeletalkey.com)
- HLA class I molecules consist of a 45-kD α chain encoded within the MHC that is noncovalently associated with the 12-kD β 2 -microglobulin chain (encoded on chromosome 15). (musculoskeletalkey.com)
- HLA class II molecules consist of noncovalently associated α (32 kD) and β (28 kD) chains, both of which are encoded within the MHC. (musculoskeletalkey.com)
- HLA class I and class II molecules are cell surface glycoproteins, anchored to the membrane by hydrophobic transmembrane segments. (musculoskeletalkey.com)
- Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. (thermofisher.com)
- Due to the artificial nature of drug/chemical-T-cell epitopes, it is not clear whether thymic selection of drug/chemical-specific T cells is a common phenomenon or remains limited to few donors or simply does not exist, suggesting T-cell receptor (TCR) cross-reactivity with other antigens. (frontiersin.org)
- When bound to the specific antigens in the kidneys and lungs, the antibodies initiate an inflammatory destruction of tissues by complement activation and recruitment of proinflammatory cells, leading to rapidly proliferative glomerulonephritis, often accompanied by pulmonary hemorrhage. (medscape.com)
- Once the anti-GBM antibodies bind to the specific GBM antigen, complement is activated. (medscape.com)
- Because the antigenic epitope is hidden within the triple helix of the collagen, an environmental factor (eg, smoking, hydrocarbon exposure) presumably is required to unmask the cryptic antigen to the immune system. (medscape.com)
- Coexistence of ANCAs (mostly myeloperoxidase [MPO-ANCAs]) with anti-GBM antibodies is thought to occur when the renal involvement in ANCA vasculitis leads to the exposure of antigens from the basement membrane and the formation of anti-GBM antibodies. (medscape.com)
- The aCL antibodies bind to b2GPI, or a complex formed by this b2GPI is a platelet adhesin glycoprotein and cardiolipin. (medscape.com)
- Background Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (bmj.com)
- The human leukocyte antigen ( HLA ) is a gene complex that encodes the major histocompatibility complex ( MHC ) proteins . (amboss.com)
- Definitions CD is an immune-mediated systemic disorder elicited by gluten and related prolamines in genetically susceptible individuals and characterised by the presence of a variable combination of gluten-dependent clinical manifestations, CD-specific antibodies, Received and accepted September 1, 2011. (vdocuments.net)
- The innate immune system develops in utero and, unlike the adaptive (acquired) immune system , does not require imprinting or adaptation to specific antigens nor does it provide permanent pathogen -specific immunity . (amboss.com)
- Activated B cells and memory T cells can recognize specific antigens on pathogens. (amboss.com)
- Applications Tested: This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
- Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. (thermofisher.com)
- During this time, the perception of CD has changed from a rather uncommon enteropathy to a common multi- organ disease with a strong genetic predisposition that is associated mainly with human leukocyte antigen (HLA)-DQ2 and HLA-DQ8. (vdocuments.net)
- HLA-DR and HLA-DQ polymorphism in human thyroglobulin-induced autoimmune thyroiditis: DR3 and DQ8 transgenic mice are susceptible. (musc.edu)
- Human aCL antibodies cause placental necrosis in BALB/c mice. (medscape.com)
- Animals immunized with aCL or with the cofactor beta-2 glycoprotein I (b2GPI) develop clinical manifestations of APS, including fetal loss, thrombocytopenia, and neurologic and behavioral dysfunction, along with elevated levels of aPL antibodies. (medscape.com)
- HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. (novusbio.com)
- The syndrome was subsequently shown to be caused by an antibody response against antigens present in the alveolar and glomerular basement membranes. (medscape.com)
- HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. (novusbio.com)
- In 1967, in a classic experiment, Lerner and colleagues transferred anti-GBM disease to monkeys by injecting them with kidney-bound antibodies from patients with anti-GBM nephritis. (medscape.com)
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. (thermofisher.com)
- The diagnosis of CD also has changed as a result of the availability of CD-specific antibody tests, based mainly on tissue transgluta- minase type 2 (TG2) antibodies. (vdocuments.net)
- In group 2, the diagnosis of CD is based on positive serology and histology. (vdocuments.net)
- Interestingly, the recent data in RA indicate that the major HLA-DR associations are with anti-CCP antibody positive disease, suggesting that control of autoantibody responses may be a primary mechanism underlying these associations in RA as well. (musculoskeletalkey.com)
- This graph shows the total number of publications written about "HLA-DR3 Antigen" by people in this website by year, and whether "HLA-DR3 Antigen" was a major or minor topic of these publications. (musc.edu)
- These antibodies predispose to clotting in vivo, predominantly by interfering with the antithrombotic role of PLs. (medscape.com)
- Antibodies reacting with the alpha-3 chain of type IV collagen can be detected in the serum and can be eluted from kidneys of patients with anti-GBM nephritis. (medscape.com)
- The 2004 National Institutes of Health/ Agency for Healthcare Research and Quality report and a systematic literature search on antibody tests for CD in paediatric patients covering the years 2004 to 2009 was the basis for the evidence-based recommendations on CD-specific antibody testing. (vdocuments.net)
- Elevation of platelet associated antibody levels in patients with chronic idiopathic thrombocytopenic purpura expressing the B8 and/or DR3 allotypes. (musc.edu)
- Women with the clinical features of APS should be tested for 3 antiphospholipid antibodies that have proven association with the diagnosis of APS: lupus anticoagulant (LAC), anticardiolipin (aCL) antibody, and anti-beta-2glycoprotein I antibody. (medscape.com)
- Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. (novusbio.com)
- A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
- Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity. (musc.edu)
- The fact that the anti-GBM antibodies are directed against the noncollagenous globular domain (NC1 domain) of the alpha-3 chain of type IV GBM collagen is well known. (medscape.com)
- Anti-GBM antibodies are directed against an epitope located at the NC1 domain at the C-terminal of the alpha-3 chain of type IV collagen. (medscape.com)
- Applications Reported: This LN3 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
- children with symptoms suggestive of CD (group 1) and asymptomatic children at increased risk for CD (group 2). (vdocuments.net)
- [ 7 ] Immunofluorescent examination of affected kidneys demonstrated that these antibodies were localized in a continuous linear deposit along the GBM. (medscape.com)