PeptidesHumansAllelesAllelePeptideTissue AntigensHumanFactors of the HLA systemEpitopesMoleculesProteinsGenotypeLociGenesImmunotherapyProteinSpecificityAnkylosingCHAINSMonoclonalEpitopeClassTransplantationSerotypeAntibodyExpressionCross reactivityCellsGenePsoriaticAssaysOrganLigandsHaplotypeReceptorsMelanomaDescriptorGenotypesDisordersPolymorphismTumorMolecularImmuneFrequenciesTransplantSpecific primersMethodsLocusMajor0.01
Peptides15
- Class I HLAs present peptides from inside the cell whereas class II HLAs present antigens from outside of the cell to T-lymphocytes. (antibodies-online.com)
- A recent study shows that individuals with the allele HLA-B*46:01 have the fewest predicted binding peptides for SARS-CoV-2, suggesting they may be particularly vulnerable to COVID-19, as they were previously shown to be for SARS. (antibodies-online.com)
- A different allele, HLA-B*15:03, showed the greatest capacity to present highly conserved SARS-CoV-2 peptides that are shared among common human coronaviruses, suggesting it could enable cross-protective T-cell based immunity. (antibodies-online.com)
- Pathogenic human thyroglobulin peptides in HLA-DR3 transgenic mouse model of autoimmune thyroiditis. (musc.edu)
- Some class I MHC genes encode nonclassical MHC molecules, such as HLA-G (which may play a role in protecting the fetus from the maternal immune response) and HLA-E (which presents peptides to certain receptors on natural killer [NK] cells). (msdmanuals.com)
- Antigens are peptides or recombinant or native dependent on the production method.Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. (hla-a.com)
- Antigens are peptides or recombinant or native dependent on the production method. (hla-a.com)
- The Recombinant HLA class I histocompatibility antigen, alpha chain G protein is a α- or alpha protein sometimes glycoprotein present in blood.Antigens are peptides or recombinant or native dependent on the production method. (histoready.com)
- BACKGROUND: Autoantibodies to cyclic citrullinated peptides (anti-CCP) are present in most patients with rheumatoid arthritis (RA), and associate with HLA-DRB1 shared epitope (SE) alleles. (ox.ac.uk)
- OBJECTIVE: To investigate reactivities of anti-CCP to various citrullinated proteins/peptides, which represent potential autoantigens in RA, and to examine the relationship between such antibodies, and their association with genetic variants within HLA-DRB1 SE alleles. (ox.ac.uk)
- In this study, the binding of flucloxacillin to immune cells was characterized and the nature of the peptides presented by HLA-B*57:01 was analyzed using mass spectrometric-based immunopeptidomics methods. (bioinfor.com)
- Of the peptides eluted from flucloxacillin-treated C1R-B*57:01 cells, 6 putative peptides were annotated as flucloxacillin-modified HLA-B*57:01 peptide ligands (data are available via ProteomeXchange with identifier PXD020137). (bioinfor.com)
- We found that HLA-C *0602 predominantly presents nonamer peptides with dominant arginine anchors at the P2 and P7 positions and a preference for small hydrophobic residues at the C terminus (PΩ). (bvsalud.org)
- To determine the structural basis of this selectivity, we determined crystal structures of HLA-C *0602 in complex with two self - peptides (ARTELYRSL and ARFNDLRFV) and an analogue of a melanocyte autoantigen (ADAMTSL5, VRSRR-abu-LRL) implicated in psoriasis . (bvsalud.org)
- A functioning TAP (transporter associated with antigen processing) molecule is required to transport these peptides into the endoplasmic reticulum, where they can interact with HLA-E. HLA-E then migrates to the cell surface, where it interacts with CD94/NKG2A receptors on natural killer cells. (ox.ac.uk)
Humans3
- Human leukocyte antigen: the major histocompatibility complex of humans. (medlineplus.gov)
- In humans, MHC proteins are encoded by the Human Leukocyte Antigen (HLA), a group of more than 200 genes located closely together on the short arm of chromosome 6. (antibodies-online.com)
- The human leukocyte antigen (HLA) system (the major histocompatibility complex [MHC] in humans) is an important part of the immune system and is controlled by genes located on chromosome 6. (msdmanuals.com)
Alleles7
- An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*03 alleles. (musc.edu)
- Alleles defined by DNA sequencing are named to identify the gene, followed by an asterisk, numbers representing the allele group (often corresponding to the serologic antigen encoded by that allele), a colon, and numbers representing the specific allele (eg, A*02:01, DRB1*01:03, DQA1*01:02). (msdmanuals.com)
- Multiple antibody reactivities to citrullinated antigens in sera from patients with rheumatoid arthritis: association with HLA-DRB1 alleles. (ox.ac.uk)
- Anti-CCP and anti-citrullinated protein antibodies were associated with HLA-DRB1*04 rather than with HLA-DRB1*01 alleles. (ox.ac.uk)
- All specific reactivities were primarily associated with the HLA-DRB1*04 alleles, suggesting common pathways of anti-citrulline immunity. (ox.ac.uk)
- Genetic susceptibility to CBD is associated with alleles of the major histocompatibility gene, human leukocyte antigen DP (HLA-DP) containing glutamic acid at the 69th position of the beta chain (HLA-DPbeta-E69). (cdc.gov)
- HLA-DRB1 alleles associate with hypercalcemia in sarcoidosis. (cdc.gov)
Allele6
- The serotype identifies the B*38 allele products of the HLA-B gene-locus. (wikipedia.org)
- Linkage studies indicate a factor in the HLA-class I region is more greatly associated, with HLA-B38 so far the only linked allele Marsh, S. G. (wikipedia.org)
- HLA-F Allele-Specific Peptide Restriction Represents an Exceptional Proteomic Footprint. (nih.gov)
- Human leukocyte antigen (HLA)-C*0602 is identified as the allele associated with the highest risk for the development of the autoimmune skin disease psoriasis . (bvsalud.org)
- The cross-linked sample DNA was hybridized with sequence-specific oligonucleotide probes to identify HLA-B27 allele sequences. (cdc.gov)
- Phenotypic and HLA-DRB1 allele characterization of Swedish cardiac sarcoidosis patients. (cdc.gov)
Peptide14
- Antigen Peptide CMV IE-1 - HLA-A*0301 (KLGGALQAK) for stimulation of antigen-specific T cells in T cell assays such as ELISPOT, ICS, cytotoxity or proliferation assays. (jpt.com)
- Find your antigen peptide and select the connected PepMix™ Peptide Pool and MHC Multimer for efficient immune monitoring, mapping of T cell epitopes or development of immunotherapy and vaccines. (jpt.com)
- The molecular basis for peptide repertoire selection in the human leucocyte antigen (HLA) C*06:02 molecule. (bvsalud.org)
- However, the diversity and mode of peptide presentation by the HLA-C *0602 molecule remains unclear. (bvsalud.org)
- Here, we describe the endogenous peptide repertoire of â ¼3,000 sequences for HLA-C *0602 that defines the peptide -binding motif for this HLA allomorph. (bvsalud.org)
- These structures revealed that HLA-C *0602 possesses a deep peptide -binding groove comprising two electronegative B- and E-pockets that coincide with the preference for P2 and P7 arginine anchors. (bvsalud.org)
- Collectively, our results provide the structural basis for understanding peptide repertoire selection in HLA-C *0602. (bvsalud.org)
- Peptide and peptide mimetic inhibitors of antigen presentation by HLA-DR class II MHC molecules. (rcsb.org)
- Molecular features of ligand binding to MHC class II HLA-DR molecules have been elucidated through a combination of peptide structure-activity studies and structure-based drug design, resulting in analogues with nanomolar affinity in binding assays. (rcsb.org)
- These studies illustrate the complementary roles played by phage display library methods, peptide analogue SAR, peptide mimetics substitutions, and structure-based drug design in the discovery of inhibitors of antigen presentation by MHC class II HLA-DR molecules. (rcsb.org)
- It binds to the leader peptide derived from the polymorphic classical major histocompatibility molecules HLA-A, HLA-B and HLA-C. This peptide binding is highly specific and stabilizes the HLA-E protein, allowing it to migrate to the cell surface. (ox.ac.uk)
- This interaction inhibits natural killer cell-mediated lysis of a cell displaying HLA-E. If the leader peptide is not present in the endoplasmic reticulum, HLA-E is unstable and is degraded before it reaches the cell surface. (ox.ac.uk)
- In damaged cells, such as virally infected or tumour cells, down-regulation of HLA-A, HLA-B and HLA-C production or inhibition of TAP prevents stabilization of HLA-E by the leader peptide. (ox.ac.uk)
- Tedopi is composed of synthetic tumoral neo-epitopes (peptide fragments) that target five tumoral antigens, permitting the activation of tumor-specific T-lymphocytes for patients who are HLA-A2 positive. (medscape.com)
Human26
- The protein is called human leukocyte antigen B27 (HLA-B27). (medlineplus.gov)
- Human leukocyte antigens (HLAs) are proteins that help the body's immune system tell the difference between its own cells and foreign, harmful substances. (medlineplus.gov)
- Histocompatibility laboratories test compatibility of transplant candidate and organ donor tissues using the human leukocyte antigen (HLA) complex. (hrsa.gov)
- Classical class I and class II Human Leukocyte Antigen (HLA) are leading candidates for infectious disease susceptibility. (antibodies-online.com)
- for example the MHC Class II antibody based on clone IVA12 recognizes the shared epitopes of human leucocyte antigen (HLA) class II molecules HLA-DP, HLA-DQ and HLA-DR heterodimeric cell surface glycoproteins comprised of an α (heavy) chain and a β (light) chain. (antibodies-online.com)
- Human Leukocyte Antigen Susceptibility Map for Severe Acute Respiratory Syndrome Coronavirus 2. (antibodies-online.com)
- HLA-DR and HLA-DQ polymorphism in human thyroglobulin-induced autoimmune thyroiditis: DR3 and DQ8 transgenic mice are susceptible. (musc.edu)
- A recent study found that patients who had a specific human leukocyte antigen (HLA) showed a "positive and statistically significant dose-dependent treatment response" to the Diamyd vaccine, especially when given four doses rather than two. (diabetesresearchconnection.org)
- Evidence for a novel HLA antigen found on human extravillous trophoblast and a choriocarcinoma cell line. (ox.ac.uk)
- Why take HLA B27 (Human Leucocyte Antigen B27)? (metropolisindia.com)
- This test helps determine whether you have human leukocyte antigen B27 (HLA-B27) on the surface of your cells and in turn it helps assess the likelihood that you may be suffering from an autoimmune disorder. (metropolisindia.com)
- The full form of HLA is Human Leukocyte Antigen and helps the body identify between its own cells and foreign body. (metropolisindia.com)
- For long term storage, keep the Recombinant human HLA class I histocompatibility antigen, alpha chain G protein frozen at -20 or -80 degrees Celsius. (histoready.com)
- The Human HLA class I histocompatibility antigen, alpha chain G protein is shipped on ice packs. (histoready.com)
- Class II Human Leukocyte Antigen (HLA) play an important role in host protection from foreign. (hla-dr.com)
- Background: Monocytic human leukocyte antigen DR (mHLA-DR) expression levels have been reported to be a marker of immunosuppression and predictors of sepsis and death. (hla-dr.com)
- Limited expression of human being leucocyte antigen-G (HLA-G) to fetal extravillous trophoblast cells which invade the decidua during implantation suggests a role for HLA-G in placentation. (biotech2012.org)
- Although expression of human leukocyte antigen (HLA)-B*57:01 increases susceptibility, little is known of the pathological mechanisms involved in the induction of the clinical phenotype. (bioinfor.com)
- Molecular basis of human natural killer cell recognition of HLA-E (human leucocyte antigen-E) and its relevance to clearance of pathogen-infected and tumour cells. (ox.ac.uk)
- HLA-E (human leucocyte antigen-E) is a conserved class I major histocompatibility molecule which has only limited polymorphism. (ox.ac.uk)
- These genes are called HLA (human leukocyte antigen) class II genotypes. (cdc.gov)
- The disease appears to be associated with the human leukocyte antigen B27 (HLA-B27) haplotype. (medscape.com)
- The technology uses the human leukocyte antigen (HLA) system, one of the keys for presenting antigens to T-lymphocytes. (medscape.com)
- An association between Human Leukocyte Histocompatibility Antigen B-27 (HLA-B27) and major arthritis syndromes such as Ankylosing Spondylitis and Reactive Arthritis is well documented (Khan et al. (cdc.gov)
- Introduction: Coeliac disease is a lifelong immune-mediated enteropathy manifested as gluten intolerance in individuals carrying specific human leukocyte antigen (HLA) molecules. (lu.se)
- Sarcoidosis development during ulcerative colitis remission in a patient with a susceptible human leukocyte antigen serotype. (cdc.gov)
Factors of the HLA system2
- A new nomenclature system for HLA antigens was recently devised by the WHO Nomenclature Committee for Factors of the HLA System. (bvsalud.org)
- See Nomenclature for Factors of the HLA System for further information on how HLA antigens are named. (bvsalud.org)
Epitopes8
- In this study, we comprehensively screened HLA-A2 restricted MAGE-A3 tumor epitopes and characterized the corresponding TCRs using in vitro artificial antigen presentation cells (APC) system, single-cell transcriptome and TCR V(D)J sequencing, and machine-learning. (thno.org)
- We identified the HLA-A2 restricted T cell epitopes from MAGE-A3 that could effectively induce the activation and cytotoxicity of CD8+ T cells using artificial APC in vitro . (thno.org)
- A cohort of HLA-A2+ NSCLC donors demonstrated that the number of epitope specific CD8+ T cells increased in NSCLC than healthy controls when measured with tetramer derived from the candidate MAGE-A3 epitopes, especially epitope Mp4 (MAGE-A3: 160-169, LVFGIELMEV). (thno.org)
- With current knowledge of epitopes, it is more accurate to describe that antibodies bind to their target epitopes on the surface of HLA molecular chains. (mundial-brasil2014.net)
- The phenomenon of cross-reactivity in HLA testing, often explained as cross-reactive groups (CREGs) of antigens with antibody, can be clearly explained now by public epitopes. (mundial-brasil2014.net)
- Since 2006, we defined and reported 194 HLA class I unique epitopes, including 56 cryptic epitopes on dissociated HLA class I heavy chains, 83 HLA class II epitopes, 60 epitopes on HLA-DRB1, 15 epitopes on HLA-DQB1, 3 epitopes on HLA-DQA1, 5 epitopes on HLA-DPB1, and 7 MICA epitopes. (mundial-brasil2014.net)
- however, it is more accurate to describe the reactivity of the antibody as binding to specific epitopes on the surface of HLA antigensepitopes are conformational amino acid arrangements and are the targets of antibodies. (mundial-brasil2014.net)
- others are public epitopes shared by two or more antigens. (mundial-brasil2014.net)
Molecules5
- Coexpression of susceptible and resistant HLA class II transgenes in murine experimental autoimmune thyroiditis: DQ8 molecules downregulate DR3-mediated thyroiditis. (musc.edu)
- Because every nucleated cell expresses class I MHC molecules, all infected cells can act as antigen-presenting cells for CD8 T cells (CD8 binds to the nonpolymorphic part of the class I heavy chain). (msdmanuals.com)
- However, the redistribution of HL-A antigens fails to provoke the redistribution of all detectable ß2-µ molecules. (silverchair.com)
- These results suggest that HL-A antigens may be associated with ß2-µ at the cell surface, but that all ß2-µ molecules are not bound to HL-A antigens. (silverchair.com)
- Amino acid sequences of the HLA molecules which greatly contributed to our understanding of antibody and antigen reactivity has been introduced since 1963 [4C12]. (mundial-brasil2014.net)
Proteins2
- This test looks for HLA-B27, which are proteins called antigens. (tidelandshealth.org)
- Flucloxacillin modification of multiple proteins was observed, providing a potential source of neoantigens for HLA presentation. (bioinfor.com)
Genotype1
- METHODS: HLA-DRB1 genotype frequencies assigned by polymerase chain reaction (PCR) amplification and sequence specific oligonucleotide probes were compared between 49 black SLE patients from Baragwanath Hospital and 87 ethnically matched controls. (ox.ac.uk)
Loci5
- Many observations point to a major role for classical HLA loci in determining susceptibility to viral infections 1 . (antibodies-online.com)
- The heavy chain of the class I molecule is encoded by genes at HLA-A, HLA-B, and HLA-C loci. (msdmanuals.com)
- Individual serologically defined antigens encoded by the class I and II gene loci in the HLA system are given standard designations (eg, HLA-A1, -B5, -C1, -DR1). (msdmanuals.com)
- The data indicate that lymphoblastoid cells express at least three sets of HLA-D region antigens that are encoded by different loci including HLA-DR and DC. (ox.ac.uk)
- HLA-B27 testing was performed on these samples using a polymerase chain reaction (PCR) to specifically replicate the DNA sequences encoding the HLA loci of interest. (cdc.gov)
Genes1
- Both polypeptide chains are encoded by genes in the HLA-DP, -DQ, or -DR region of chromosome 6. (msdmanuals.com)
Immunotherapy2
- The possibility of interactions between HLA type and outcome should be considered in future immunotherapy trials. (northwestern.edu)
- These findings may help to advance the development of antigen-specific immunotherapy options for individuals with type 1 diabetes leading to improved treatment or management of the disease. (diabetesresearchconnection.org)
Protein2
- HLA-B27 is a blood test to look for a protein that is found on the surface of white blood cells. (medlineplus.gov)
- HLA-B27 is a specific protein that is found on cell surfaces. (metropolisindia.com)
Specificity2
- that is, different beta-2 polypeptides were associated with the same HLA-DR specificity in different geographical populations. (ox.ac.uk)
- Determining specificity of the anti-HLA antibody has advanced in recent years using recombinant CHMFL-ABL-039 HLA single antigens (SA) coated on color-coded Luminex beads [3]. (mundial-brasil2014.net)
Ankylosing1
- People with HLA-B27 have an increased likelihood of developing autoimmune diseases such as ankylosing spondylitis, reactive arthritis, juvenile rheumatoid arthritis and isolated acute anterior uveitis. (metropolisindia.com)
CHAINS5
- Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. (nih.gov)
- Figure J. Epitope 2018 shared by the alpha chains of the DQ4, 5, 6 antigens and defined by Glutamine (Q) at position 53. (mundial-brasil2014.net)
- Figure K. Epitope 2002 shared exclusively by the beta chains of the DQ4 antigen and defined by Leucine (L) in position 56. (mundial-brasil2014.net)
- Figure L. Epitope 2010 shared by the beta chains of DQ antigens DQ4, 5, 6, 8, 9 and defined by 45G+46V. (mundial-brasil2014.net)
- Figure N. Epitope 2006 shared by DQB1?03:01 (DQ7), DQB1?03:02 (DQ8), DQB1?03:03 (DQ9) and defined by Proline (P) at position 55 on the beta chains of the DQ antigens. (mundial-brasil2014.net)
Monoclonal3
- Two-dimensional gel electrophoresis of material immunoprecipitated from radiolabeled cells by anti-HLA class II monoclonal antibodies has been a useful technique but the patterns observed have varied considerably between laboratories. (ox.ac.uk)
- This antigen is an electrophoretically non-polymorphic glycoprotein of approximately 40,000 molecular weight, which is found in association with beta 2 microglobulin, and which is detected by monoclonal antibodies recognizing monomorphic determinants of HLA Class I. Elucidation of the nature and origin of this molecule may provide valuable information regarding the immune barrier that exists between mother and fetus. (ox.ac.uk)
- Circulation cytometry Monoclonal antibodies (mAbs) used in this study to bind HLA-G were G233 (Loke (1993) by amplifying a 141 or 155 bp region spanning the polymorphism using the primers 5′-GTAGTGTGAAACAGCTGCCC-3′ and 5′-AAGGAATGCAGTTCAGCATGA-3′ and resolving products by electrophoresis having a 3% low-melting-point agarose gel. (biotech2012.org)
Epitope4
- Figure G. Epitope 5037 shared by the HLA C-locus antigens Cw4, Cw6, Cw17, Cw18 and defined by 73A+77N. (mundial-brasil2014.net)
- Figure H HLA class II DQB epitope 2007 shared by DQ4,5,6 antigens and define by 52P+55R on the beta chain of the DQ antigens. (mundial-brasil2014.net)
- Figure M. Epitope 2022 exclusive to DQB1?05:01 chain on the DQ5 antigen and defined by 125S+126Q. (mundial-brasil2014.net)
- Structure of TCR and antigen complexes at an immunodominant CTL epitope in HIV-1 infection. (expasy.org)
Class9
- HLA class II antigens associated with systemic lupus erythematosus in black South Africans. (ox.ac.uk)
- OBJECTIVE: To assess the associations of HLA class II antigens with systemic lupus erythematosus (SLE) in black South Africans. (ox.ac.uk)
- Simplification of two-dimensional gel patterns of HLA class II antigens. (ox.ac.uk)
- classical HLA Class I antigens are not expressed on these cells. (ox.ac.uk)
- The number, class, and cumulative strength of HLA-DSA determined by SAFB, and prior sensitizing events were not predictive for the occurrence of AMR. (unibas.ch)
- Given these indications that the reported findings of IFN-γ and IL-8 production by dNK cells are in response to stimulation we tested dNK cell degranulation when cultured with HLA class I-null target cells (Fig.?4C). (biotech2012.org)
- This gene belongs to the HLA class I heavy chain paralogues. (nih.gov)
- HLA class II variants defined by next generation sequencing are associated with sarcoidosis in Korean patients. (cdc.gov)
- HLA class I and class II antigens in sarcoidosis. (cdc.gov)
Transplantation2
- Hence the outcome of reduced intensity HSCT with lymphodepleting antibodies in older patients with intermediate/high-risk hematological malignancies appears comparable to that obtained with fully ablative transplantation in younger patients, even when these older recipients lack HLA-identical sibling donors. (nih.gov)
- 1. Introduction Sensitization to HLA antigens in organ transplant patients causes graft rejection, according to the humoral theory of transplantation [1]. (mundial-brasil2014.net)
Serotype1
- HLA-B38 (B38) is an HLA-B serotype. (wikipedia.org)
Antibody4
- The aims of this study were to investigate to which extent HLA-DSA detected by SAFB represent a risk for antibody-mediated rejection (AMR) and diminished allograft survival, and to define HLA-DSA characteristics predictive for AMR. (unibas.ch)
- The antibody-induced redistribution of ß2-microglobulin (ß2-µ) and HL-A antigens on the surface of living lymphocytes was studied by immunofluorescence. (silverchair.com)
- Advances in testing assays and amino acid sequencing of HLA along with computer software contributed further to the understanding of antibody-antigen reactivity. (mundial-brasil2014.net)
- Tests for antibodies to liver-kidney microsome type 1 (anti-LKM-1), soluble liver antigen (anti-SLA), and liver cytosol type 1 (anti-LC1) may be helpful if antinuclear antibody (ANA) and anti-smooth muscle antibody (ASMA) test results are negative. (medscape.com)
Expression3
- The effects of treatment on relapse-free survival (RFS) and overall survival (OS) were evaluated, and prespecified analyses investigated associations between treatment and HLA expression. (northwestern.edu)
- There was no impact of HLA-A2 and/or HLA-Cw3 expression on observation arm patients. (northwestern.edu)
- Correlation of alteration of HLA-F expression and clinical characterization in 593 brain glioma samples. (nih.gov)
Cross reactivity2
- they were specific for their respective antigen and displayed limited cross reactivity. (ox.ac.uk)
- The reactivity of anti-HLA antibodies with HLA antigens and the phenomenon of cross-reactivity has been the subject of investigation for decades. (mundial-brasil2014.net)
Cells11
- The results confirm that the positional variation of one beta polypeptide (namely beta-1) correlated with the HLA-DR allospecificity and was not influenced by the ethnic origin of the individual donating the cells. (ox.ac.uk)
- that is, apparently the same beta-3 polypeptide was associated with different HLA-DR specificities provided the cells were of the same ethnic origin. (ox.ac.uk)
- We then measured functional DB07268 reactions of dNK cells with LILRB1 when stimulated by HLA-G in both monomeric and dimeric conformations. (biotech2012.org)
- Degranulation interferon-γ and interleukin-8 production by dNK cells freshly isolated from your 1st trimester implantation site were either undetected or not affected by HLA-G. These findings should be considered when inferring the activity of cells NK cells from results acquired with cell lines peripheral NK or cultured dNK cells. (biotech2012.org)
- Pulse chase and immunoprecipitation 721.221 cells transfected with HLA-G were starved for 30 min in the medium lacking cysteine and methionine labelled for 20 min with 0.1 mCi/ml l-[35S]methionine and l-[35S]cysteine (Promix GE Healthcare) and chased in a regular medium for the indicated times. (biotech2012.org)
- IL-8 production was primarily seen in HLA-DR+ cells which are predominantly macrophages in our decidual leucocyte preparations (Gardner and Moffett 2003 IFN-γ was produced after 6 h stimulation with PMA confirming that dNK cells can produce this cytokine. (biotech2012.org)
- Decidual leucocytes from normal first trimester pregnancies were analysed immediately after isolation by intracellular flow cytometry for the production of IFN-γ … We then sought to investigate modulation by HLA-G of dNK cell degranulation responses to target cells. (biotech2012.org)
- Neither monomeric nor dimeric forms of HLA-G affected degranulation of dNK cells (Fig.?5D). (biotech2012.org)
- Degranulation of PBNK cells was inhibited by HLA-G consistent with previous reports (Perez-Villar by flow cytometry on EVT cells isolated from 70 normal first trimester pregnancies. (biotech2012.org)
- To conclude, we have characterized naturally processed drug-haptenated HLA ligands presented on the surface of antigen presenting cells that may drive drug-specific CD8+ T-cell responses. (bioinfor.com)
- Under these circumstances, HLA-E does not reach the cell surface and the cell is then vulnerable to lysis by natural killer cells. (ox.ac.uk)
Gene3
- Another gene, possibly HLA-DQB1*02, not linked to DR2 is involved in the subset of patients exhibiting Ro antibodies. (ox.ac.uk)
- Variation in the HLA-F gene locus with functional impact is associated with pregnancy success and time-to-pregnancy after fertility treatment. (nih.gov)
- The vaccine is effective for patients who express the HLA-A2 gene, which is present in around half of the population. (medscape.com)
Psoriatic1
- A higher frequency of HLA-B38 was noted psoriatic arthritis patients with erythroderma. (wikipedia.org)
Assays1
- BACKGROUND: Defining the clinical relevance of donor-specific HLA-antibodies detected by single-antigen flow-beads (SAFB) is important because these assays are increasingly used for pretransplant risk assessment and organ allocation. (unibas.ch)
Organ4
- HLA testing is also used to match donated tissue with a person's tissue who is getting an organ transplant. (medlineplus.gov)
- There is no current requirement to communicate HLA typing changes to transplant programs or Organ Procurement Organizations (OPOs). (hrsa.gov)
- If you need an organ or tissue transplant and your HLA antigens are not compatible with those of your donor, your body could reject the transplant. (tidelandshealth.org)
- CONCLUSIONS: These results support the utility of SAFB for pretransplant risk assessment and organ allocation, and suggest that improvement of the positive predictive value of HLA-DSA defined by SAFB will require an enhanced definition of pathogenic factors of HLA-DSA. (unibas.ch)
Ligands2
Haplotype1
- 2 These observations point towards a potential influence of different HLA composition - the haplotype - in the present SARS-CoV-2 pandemic. (antibodies-online.com)
Receptors1
Melanoma2
- An analysis of mature data from S9035 indicates a significant OS benefit from adjuvant vaccine therapy for patients with HLA-A2- and/or HLA-Cw3-expressing melanoma. (northwestern.edu)
- Further investigations of melanoma-associated antigens present in Melacine and presented by HLA-A2 and HLA-Cw3 may be warranted. (northwestern.edu)
Descriptor2
- HLA-DR3 Antigen" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (musc.edu)
- In order to make 2012 MeSH conform to the new HLA system over 370 MeSH concepts were updated and 25 new HLA-related descriptor classes were created. (bvsalud.org)
Genotypes1
- HLA-DQA1 and -DQB1 genotypes were also assigned in 45 of the SLE patients and 74 controls by PCR using sequence specific primers. (ox.ac.uk)
Disorders2
- 2009). Some studies indicate an increase in mortality among persons who have HLA-B27 associated disorders, however there are no mortality studies that relate directly to the HLA-B27 trait itself (Zochling and Braun, 2008). (cdc.gov)
- A U.S. national-level HLA-B27 prevalence estimate is required for public health planning purposes because of the recent significant improvements in the medical therapy for HLA-B27 related disorders. (cdc.gov)
Polymorphism3
- Biochemical analysis of the polymorphism of three HLA-D region antigens. (ox.ac.uk)
- The polymorphism has been examined using lymphoblastoid cell lines that were derived from different geographical populations (North European and Italian) and that expressed various HLA-DR alloantigenic specificities. (ox.ac.uk)
- The single-nucleotide polymorphism in the 3′-UTR of HLA-G in the miR-148a/b-binding site was typed by PCR with the primers 5′-TCTCCTGCAACAAATCAGCAC-3′ and 5′-AAGGGGCTGGGATGTC-TCCG-3′ and sequencing of products using the same primers and an ABI 3730 DNA analyzer (Applied Biosystems). (biotech2012.org)
Tumor1
- This work identified candidate TCRs potentially suitable for TCR-T design targeting HLA-A2 restricted MAGE-A3 tumor antigen. (thno.org)
Molecular1
- The molecular mechanisms underlying this function of HLA-E have been revealed by crystallographic studies of the structure of HLA-E. (ox.ac.uk)
Immune3
- However, systematic analysis of T cell immune responses to MAGE-A3 antigen and corresponding antigen-specific TCR is still lacking. (thno.org)
- The aim of integrating these five antigens is to prevent immune escape. (medscape.com)
- They had an HLA-A2 phenotype, as determined by a blood draw to determine whether their immune system could respond to the vaccine. (medscape.com)
Frequencies1
- Frequencies of HLA and Gm immunogenetic markers in Kaposi's sarcoma. (musc.edu)
Transplant7
- Patient safety is at risk when transplant programs are not aware of HLA typing changes. (hrsa.gov)
- If a histocompatibility lab becomes aware of a discrepancy in a candidate or recipient's HLA typing, they will notify the transplant program within five days of determining the correct typing and provide documentation of the corrected typing. (hrsa.gov)
- This is an important policy to improve communication between HLA laboratories, OPO's and transplant programs and ASHI strongly supports this policy. (hrsa.gov)
- Your donor's HLA antigens must match yours for the transplant to have a chance to be successful. (tidelandshealth.org)
- METHODS: In this retrospective study of 334 patients with negative complement-dependent cytotoxicity crossmatches, day-of-transplant sera were analyzed by SAFB, HLA-DSA determined by virtual crossmatching, and the results correlated with the occurrence of AMR and allograft survival. (unibas.ch)
- Sensitization is almost ubiquitous as anti-HLA antibodies are found in almost all sera of transplant recipients. (mundial-brasil2014.net)
- Sensitization is almost ubiquitous as it is evident in the detection of anti-HLA antibodies in the sera of recipientsin one study, almost all patients waiting for regraft of a kidney transplant have anti-HLA antibodies [2]. (mundial-brasil2014.net)
Specific primers1
- Exons 2 and 3 of the HLA-B locus were amplified with locus specific primers and the amplified DNA was arrayed onto seven replicate nylon membranes and immobilized by UV cross-linking. (cdc.gov)
Methods1
- Please see the HLA-B27 component Laboratory Manual for more detailed documention of laboratory testing methods and quality control procedures. (cdc.gov)
Locus1
- The positional variation of a second beta polypeptide (namely beta-2) was consistent with strong linkage dis-equilibrium between the corresponding genetic locus and the HLA-DR locus. (ox.ac.uk)
Major2
- This graph shows the total number of publications written about "HLA-DR3 Antigen" by people in this website by year, and whether "HLA-DR3 Antigen" was a major or minor topic of these publications. (musc.edu)
- A group of antigens that includes both the major and minor histocompatibility antigens. (bvsalud.org)
0.011
- HLA-DQB1*0201 was not associated with development of the disease itself, but was associated with the presence of Ro antibodies (p = 0.01). (ox.ac.uk)