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  • immunology
  • The solution of peptide/MHC-I structures answered fundamental questions in immunology, including how a single receptor can simultaneously recognize both self (MHC) and non-self (peptide) in antigen recognition ( 2 ). (frontiersin.org)
  • epitope
  • Expression of this monochain restores a sizable peripheral CD8(+) T cell repertoire essentially educated on the transgenic human molecule.Interestingly, the CTL response to influenza A virus is mostly, if not exclusively, directed to the 58-66 matrix peptide which is the HLA-A2.1-restricted immunodominant epitope in humans.Such mice might constitute a versatile animal model for the study of HLA-A2.1-restricted CTL responses of vaccine interest. (nih.gov)
  • Interestingly, the CTL response to influenza A virus is mostly, if not exclusively, directed to the 58-66 matrix peptide which is the HLA-A2.1-restricted immunodominant epitope in humans. (nih.gov)
  • HLA-A2 and B35 restricted hantaan virus nucleoprotein CD8+ T-cell epitope-specific immune response correlates with milder disease in hemorrhagic fever with renal syndrome. (nih.gov)
  • However, the T cell epitopes of the HTNV restricted by the HLA alleles and the role of epitope-specific T cell response after HTNV infection remain largely unexplored. (nih.gov)
  • Two epitopes aa129-aa137 and aa131-aa139 restricted by HLA-A2 and B35, respectively, were selected to evaluate the epitope-specific CD8(+) T-cell response. (nih.gov)
  • C) The associations between the frequencies of the epitope-specific CD8+ T cells at the acute stage and the peak level of serum creatinine or nadir platelet counts during the hospitalization in 18 donors with HLA-A2 and in 8 donors with HLA-B35 (Spearman correlation test). (nih.gov)
  • We compared the epitope-specific CD8+ T-cell frequencies between patients with mild/moderate HFRS and those with severe/critical HFRS at the earliest available acute stage time point in the 18 patients with HLA-A2 and in the 8 patients with HLA-B35 from whom PBMCs had been collected. (nih.gov)
  • HCV1406 was subsequently shown to recognize the HCV nonstructural protein 3 (NS3):1406-1415 epitope with high specificity when presented by HLA-A2. (pnas.org)
  • We show that NS3/HLA-A2 recognition by the HCV1406 TCR is critically dependent on features unique to both the allo-MHC and the NS3 epitope. (pnas.org)
  • Human transporters associated with antigen processing (TAPs) select epitope precursor peptides for processing in the endoplasmic reticulum and presentation to T cells. (nih.gov)
  • Low TAP affinities preclude minimal epitope access to the endoplasmic reticulum (ER) and assembly with HLA-A2 in vitro, as well as presentation by minigene-expressing cells to cytotoxic T lymphocytes. (nih.gov)
  • It has been previously demonstrated that circulating CD8 + cytotoxic T lymphocytes (CTLs) in patients with HAM/TSP react against HTLV-1 protein products ( 10 ), and an immunodominant HLA-A2-restricted epitope (HTLV-1 Tax11-19) has been well characterized ( 11 ). (pnas.org)
  • To address a destructive role for autoreactive CD8 T-cells in human disease, we assessed the pathogenicity of a CD8 T-cell clone derived from a T1D donor and specific for an HLA-A2-restricted epitope of islet-specific glucose-6-phosphatase catalytic-subunit related protein (IGRP). (jove.com)
  • Epitopes
  • Five well-conserved novel CD8(+) T-cell epitopes of the HTNV nucleoprotein restricted by the most popular HLA alleles in Chinese Han population were defined with interferon-γ enzyme-linked immunospot assay in 37 patients infected with HTNV during hospitalization. (nih.gov)
  • The novel HLA class I restricted HTNV nucleoprotein epitopes-specific CD8(+) T-cell responses would be closely related with the progression and the severity of the disease, which could provide the first step toward effective peptide vaccine development against HTNV infection in humans. (nih.gov)
  • Recently, we identified two HLA-A2-restricted T-cell epitopes derived from the MUC1 protein. (aacrjournals.org)
  • To extend the possible use of MUC1-derived T-cell epitopes in immunotherapeutic approaches, we screened the expression of MUC1 on normal hematopoietic cells (14) as well as on various hematological malignancies using monoclonal antibodies specific for the MUC1 tumor antigen. (aacrjournals.org)
  • To prove the presentation of T-cell epitopes by the malignant cells, we induced MUC1-specific CTLs in vitro using peptide-pulsed DCs as antigen-presenting cells. (aacrjournals.org)
  • We studied two naturally immunodominant viral epitopes presented by HLA-A2 that display very low affinities for human TAP. (nih.gov)
  • Analysis of TAP affinities of a panel of viral epitopes suggests that TAP selection of precursors may be a common phenomenon for HLA-A2-presented epitopes. (nih.gov)
  • 250 target cells can be assessed at once, the technique allows the monitoring of T cell responses against several antigen epitopes at several concentrations and in multiple replicates. (jove.com)
  • Molecule
  • Complexes of five peptides (from HIV-1, influenza A virus, HTLV-1, and hepatitis B virus proteins) bound to the human class I MHC molecule HLA-A2 have been studied by X-ray crystallography. (rcsb.org)
  • That figure shows a little bit of undefined, unresolved mist (in pink) in the middle of the reasonably well-defined HLA-A2 molecule. (iayork.com)
  • Here we describe the x-ray crystal structure of a human class I MHC molecule, HLA-Aw68, from which the alpha 3 domain has been proteolytically removed. (rcsb.org)
  • A decameric peptide complexed with the intact HLA-Aw68 is seen to bind to the proteolized molecule in the conventional manner, demonstrating that the alpha 3 domain is not required for the structural integrity of the molecule or for peptide binding. (rcsb.org)
  • melanoma
  • Although such tolerance mechanisms have emerged as formidable barriers to immunotherapy, as discussed below, our knowledge of tumor-associated antigens for melanoma and other tumor types has provided potential molecular targets for T cell-based immunotherapy, and has allowed for the design of strategies to break immune tolerance for patients with cancer. (aacrjournals.org)
  • murine
  • The role of lipoprotein-associated phospholipase A2 in a murine model of experimental autoimmune uveoretinitis. (jove.com)
  • gene
  • A first antigen recognized by such CTL clones was previously shown to be encoded by the tyrosinase gene. (rupress.org)
  • We report here the identification of another gene that also directs the expression of an antigen recognized on most melanomas by CTL clones that are restricted by HLA-A2. (rupress.org)
  • HLA class I histocompatibility antigen, A-2 alpha chain (365 aa, ~41 kDa) is encoded by the human HLA-A gene. (healthmatics.info)
  • We included in the assay the same tumor transduced with the CD80 gene to provide "costimulation" for T cell activation (MS-A2/CD80). (nih.gov)
  • We and others have successfully generated and infused T-cells specific for Epstein Barr virus (EBV), cytomegalovirus (CMV) and Adenovirus (Adv) using monocytes and EBV-transformed lymphoblastoid cell (EBV-LCL) gene-modified with an adenovirus vector as antigen presenting cells (APCs). (jove.com)
  • The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways. (jove.com)
  • peripheral blood
  • Although such approaches are relatively nontoxic and have resulted in increased numbers of circulating antigen-specific T cells in peripheral blood (especially after long series of vaccinations over many months), rarely have they led to objective regressions of bulky, established solid tumors ( 18 - 23 ). (aacrjournals.org)
  • Objectives: The main aim of this study was to investigate the rate of HLA-A2 expression on peripheral blood mononuclear cells (PBMCs) of patients with OBI. (ac.ir)
  • HLA-A2/IGRP tetramer staining revealed a higher frequency of IGRP-specific CD8 T-cells in the peripheral blood of recent onset human individuals than of healthy donors. (jove.com)
  • PBMCs
  • Results: Flow cytometric analysis indicated that 19 (33.3%) OBI patients and 28 (28%) controls expressed HLA-A2 antigen on their PBMCs. (ac.ir)
  • Conclusions: According to these results it can be concluded that decreased intensity of HLA-A2 on the PBMCs of OBI patients may lead to resistance of HBV in the patients. (ac.ir)
  • Presentation
  • Conflicts between TAPs and MHC preferences for COOH-terminal peptide residues in rodent cells strongly reduce the efficiency of MHC class I antigen presentation. (nih.gov)
  • bind
  • Recently, tetrameric major histocompatibility complex (MHC) -peptide complex crosslinked by streptavidin were shown to bind stably to antigen-specific T cells based on the increased avidity afforded by polyvalency ( 19 ). (pnas.org)
  • metabolism
  • Upon metabolism and degradation of HLA, β2M is dissociated from the heavy chain and is released in its free form into the extracellular fluids ( 6 ). (aacrjournals.org)
  • specific
  • Therapeutic prostate-specific antigen (PSA) -targeted poxviral vaccines for prostate cancer have been well tolerated. (nih.gov)
  • activation
  • Macrophage activation is, in part, regulated via hydrolysis of oxidised low density lipoproteins by Lipoprotein-Associated phospholipase A2 (Lp-PLA2), resulting in increased macrophage migration, pro-inflammatory cytokine release and chemokine expression. (jove.com)
  • alpha
  • A large groove between the alpha-helices provides a binding site for processed foreign antigens. (nih.gov)