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HistonesHistone DeacetylasesAcetylationHistone AcetyltransferasesHistone Deacetylase InhibitorsChromatinMethylationHistone-Lysine N-MethyltransferaseNucleosomesHistone Deacetylase 1Histone DemethylasesHistone ChaperonesHistone CodeHydroxamic AcidsChromatin Assembly and DisassemblyHistone Deacetylase 2LysineEpigenesis, GeneticProtein MethyltransferasesHeterochromatinChromatin ImmunoprecipitationTranscription, GeneticChromosomal Proteins, Non-HistoneJumonji Domain-Containing Histone DemethylasesGene SilencingAcetyltransferasesMolecular Sequence DataProtein BindingNuclear ProteinsSaccharomyces cerevisiae ProteinsAmino Acid SequenceProtamine KinaseRepressor ProteinsTranscription FactorsProtein Processing, Post-TranslationalDNA-Binding ProteinsDNA MethylationSaccharomyces cerevisiaeHeLa Cellsp300-CBP Transcription FactorsEuchromatinPhosphorylationDNAMethyltransferasesGene Expression RegulationMicrococcal NucleaseCentromereCell LineProtein Structure, TertiaryCell Cycle ProteinsCell CyclePolycomb Repressive Complex 2MutationButyratesBase SequenceGene Expression Regulation, FungalMitosisOxidoreductases, N-DemethylatingRNA Polymerase IINucleosome Assembly Protein 1Binding SitesProtein-Arginine N-MethyltransferasesPolycomb-Group ProteinsCell Line, TumorChickensPromoter Regions, GeneticTranscriptional ActivationDNA DamageRetinoblastoma-Binding Protein 4ChromosomesRNA, MessengerEnzyme InhibitorsDrosophila ProteinsSirtuin 2UbiquitinationSin3 Histone Deacetylase and Corepressor ComplexButyric AcidDNA ReplicationCell NucleusSubstrate SpecificitySirtuinsEpigenomicsBlotting, WesternValproic AcidCells, CulturedElectrophoresis, Polyacrylamide GelRNA InterferenceChromatin Assembly Factor-1Sea UrchinsMultiprotein ComplexesSequence Homology, Amino AcidRibosomal Protein S6 Kinases, 90-kDaSerineNucleoplasminsProtein-Serine-Threonine KinasesDrosophila melanogasterS PhaseCREB-Binding ProteinProtein KinasesHigh Mobility Group Proteins
MethylationLysineProteinsAcetylationPeptideGenesGlobular domainLevels of histoneModulating chromatin structureEnzymesNucleosomeModificationsMitochondrialOctamerVariantsDeacetylaseSpecific histoneRecombinantTetramerAntibodyDemethylaseHighly conservedPhosphoLys9RepressiveLinkerResiduesMammalianSubunitTranscriptionModificationEpigeneticsInhibitorChromatin organizationSpeciesAbsorbanceH3.2PhosphorylationMethylatesRegulationHumanPrimary AntibodiesSynthesisThermodynamic2019Yeast2018HATsHomolog
Methylation16
Lysine9
  • Acetylation of histone H3 at several lysine positions in the histone tail is performed by histone acetyltransferase enzymes (HATs). (wikipedia.org)
  • The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression. (abcam.com)
  • Lysine 4 of Histone H3 (1-20) K4Me3, K9Ac-GG-[Lys(5-FAM)] has been tri-methylated, lysine 9 has been acetylated and the C-terminal has been labelled with 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag. (crbdiscovery.com)
  • Due to histones containing a large number of lysine and arginine residues they have a positive net charge which interacts in an electrostatic manner with the negatively charged phosphate groups in DNA. (crbdiscovery.com)
  • Chandrasekaran, R., and Thompson, M. (2007) Polybromo-1-bromodomains bind histone H3 at specific acetyl-lysine positions. (mtu.edu)
  • Polybromo-1 bromodomains bind histone H3 at specific acetyl-lysine positions. (mtu.edu)
  • Enhancer of zeste homolog 2 (EZH2), one of the core components of PRC2, is a methyltransferase possessing the enzymatic activity to generate di/trimethylated lysine 27 in histone H3 [ 2 ]. (hindawi.com)
  • Ketamine treatment increased the binding of NF‑κB and permissive histone H3 lysine‑4 (H3K4)m3, but caused a decrease in the repressive histone H3K27m3 and H3K36m3 on the COX‑2 promoter ranging from ‑1,522 to ‑1,331 bp as determined by a chromatin immunoprecipitation assay. (spandidos-publications.com)
  • Globally elevated levels of histone H3 lysine 9 trimethylation in early infancy are associated with poor growth trajectory in Bangladeshi children. (cdc.gov)
Proteins9
  • Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. (wikipedia.org)
  • The transcriptional activation or silencing of the chromatin is controlled by ATP-dependent chromatin remodelling factors and histone modifying enzymes which target histone proteins. (crbdiscovery.com)
  • Total proteins were precipitated in 95% ethanol at components, including - and -globin chains, histones, and -20°C and recovered by centrifugation. (cdc.gov)
  • The multi-component Nox2 has both trans-membrane and cytosolic proteins, and the small G-protein, Ras-related C3 botulinum toxin substrate 1 (Rac1), is an integral cytosolic component for its activation 8 . (nature.com)
  • The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). (activemotif.com)
  • Recombinant Histone H3 phospho Ser10 (H3S10ph) proteins are generated using expressed protein ligation (EPL) technology. (activemotif.com)
  • The main proteins found in chromatin are called histones. (hstalks.com)
  • They are small, basic proteins called H3, H4, H2A, and H2B for the core histones and H1 for the linker histone. (hstalks.com)
  • Polycomb Group (PcG) proteins are a family of protein complex that regulate gene expression, especially repressing gene transcription [ 1 ]. (hindawi.com)
Acetylation10
  • Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. (abcam.com)
  • Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. (abcam.com)
  • Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) allows the user to colorimetrically detect and quantify if histone H3 is acetylated. (abcam.com)
  • ab115124 is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells. (abcam.com)
  • Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (ab115124) Nowacka-Woszuk, Joanna et al. (abcam.com)
  • The differences in H3 acetylation level in fetal livers (female fetuses aged 19 dpc) from F1, F2, and F3 generations. (abcam.com)
  • Following acetylation, newly synthesized H3-H4 is directly transferred from the histone chaperone anti-silencing factor 1 (Asf1) to chromatin assembly factor 1 (CAF-1), another histone chaperone that is critical for the deposition of H3-H4 onto replicating DNA. (elsevierpure.com)
  • A thermodynamic model for acetylation-dependent polybromo-histone interactions. (mtu.edu)
  • Thermodynamic analysis of acetylation-dependent Pb1 bromodomain - histone H3 interactions. (mtu.edu)
  • Deciphering the histone acetylation code for human Polybromo-1. (mtu.edu)
Peptide9
  • This peptide is Histone 3, with amino acid residues 21 to 44. (eurogentec.com)
  • Butyryl/Crotonyl-Histone H3 (Lys9) (E9N8C) Rabbit mAb specificity was determined by peptide ELISA. (cellsignal.com)
  • The graph depicts the binding of the antibody to pre-coated crotonyl histone H3 (Lys9) peptide in the presence of increasing concentrations of various competitor peptides. (cellsignal.com)
  • As shown, the butyryl and crotonyl histone H3 (Lys9) peptides completely competed away the antibody and there was partial competition with the crotonyl histone H3 (Lys27) peptide. (cellsignal.com)
  • Synthesized histone H3 peptide corresponding to residues within 1-21 of human histone H3, biotin conjugated. (epigentek.com)
  • This peptide is histone H3 (1-50) biotinylated via the C-terminal GGK linker. (bestbiochem.com)
  • When the peptide purity is greater than 95%, the peptide is dissolved in distilled water at a concentration of 1 mg/ml, and lyophilized again into a powder form. (bestbiochem.com)
  • In EPL, the histone globular domain is ligated to a peptide that contains the N-terminal histone tail with the desired site-specific modification. (activemotif.com)
  • The purified protein is subsequently ligated to a N-terminal histone tail peptide containing phosphorylated serine 10 via a native peptide bond. (activemotif.com)
Genes6
  • Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. (wikipedia.org)
  • Consistent with this idea, we confirm that CAF-1 synchronously binds two H3-H4 dimers derived from two different histone genes in vivo. (elsevierpure.com)
  • Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin. (broadinstitute.org)
  • Active genes are tri-methylated at K4 of histone H3. (broadinstitute.org)
  • Genome-wide studies in Saccharomyces cerevisiae , for example, have revealed that promoters of most actively transcribed genes are depleted of nucleosomes and that histone density is inversely proportional to the transcription rate within coding regions, suggesting that nucleosomes are dynamically disassembled and reassembled at each passage of the polymerase ( 16 ). (asm.org)
  • 5% of GBMs and include disorders such as Li-Fraumeni syndrome (TP53 mutation), Turcot syndrome (biallelic mutation of mismatch repair genes), and neurofibromatosis type 1 (NF1 mutation). (medscape.com)
Globular domain1
  • Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. (wikipedia.org)
Levels of histone1
  • In UV models of ALCs and human lens epithelial cell lines, the expression levels of histone H3, cell apoptosis factors (Bax/Bcl-2, cleaved caspase-3), and inflammation factors (interleukin-6, tumor necrosis factor-α) were all up-regulated. (techscience.com)
Modulating chromatin structure1
Enzymes4
  • Histone H4 is also modified by various enzymes and these modifications have been shown to be important in gene regulation. (neb.com)
  • Histone H4 is also modified by various enzymes and can act as a substrate for them. (neb.com)
  • Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro . (activemotif.com)
  • Chromatin endogenous cleavage (ChEC) and psoralen experiments indicated that active rDNA is devoid of histones ( 23 ), whereas chromatin immunoprecipitation (ChIP) and genetic studies led to the conclusion that active rDNA has at least a few histones and is associated with chromatin-remodeling enzymes ( 15 , 31 , 34 ). (asm.org)
Nucleosome8
  • The N-terminus of H3 protrudes from the globular nucleosome core and is susceptible to post-translational modification that influence cellular processes. (wikipedia.org)
  • Histone H3 (1-20) K4Me3, K9Ac-GG-[Lys(5-FAM)] is derived from Histone 3 (H3) which is one of the four core histones fundamental for compacting eukaryotic DNA into the nucleosome. (crbdiscovery.com)
  • The nucleosome arises when 147 base pairs of DNA wrap around a H3-H4 tetramer and two H2A-H2B dimers, forming the histone octamer core. (crbdiscovery.com)
  • Both H4 and H3 are highly conserved and perform roles in binding to segments of DNA which enter and leave the nucleosome and in chromatin formation. (crbdiscovery.com)
  • Together, the data illustrate a clear mechanism for CAF-1- associated H3-H4 chaperone activity in the context of de novo nucleosome (re)assembly following DNA replication. (elsevierpure.com)
  • Histone H3 is one of the core components of the nucleosome. (activemotif.com)
  • Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. (activemotif.com)
  • First, you have the DNA in the form of a helix, which is wrapped around the core histones to form the nucleosome-the sort of tennis ball that was represented before- which then folds up further to give chromatin fibers, which further fold up and then organize in the nucleus to form different domains. (hstalks.com)
Modifications1
  • Although it is clear that switching among BESs does not involve DNA rearrangements and that regulation is probably epigenetic, it remains unknown why BESs are transcribed by Pol I and what roles are played by chromatin structure and histone modifications. (asm.org)
Mitochondrial1
  • Moreover, deletion of p62 did not change the levels of crystallins, histone H3, the mitochondrial import receptor subunit TOM20 homolog, or the abundance of nuclei and nuclear fragments in the lenses of homozygous Cx50D47A mice. (molvis.org)
Octamer1
  • Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer (1,2). (neb.com)
Variants5
  • The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. (wikipedia.org)
  • Mammalian cells have seven known sequence variants of histone H3. (wikipedia.org)
  • Histone variants from different organisms, their classification and variant specific features can be found in "HistoneDB - with Variants" database. (wikipedia.org)
  • In this talk, I'd like to discuss with you the issue concerning histone dynamics, their heritability, and their existence in the form of variants. (hstalks.com)
  • We describe genetic alterations in isocitrate dehydrogenase, ATRX, the telomerase promoter, and histone H3 variants that promote GBM tumorigenesis and have altered GBM categorization. (medscape.com)
Deacetylase1
Specific histone1
  • specific histone deposition pathways for each variant? (hstalks.com)
Recombinant8
  • Here, we show that CAF-1 binds recombinant H3-H4 with 10- to 20-fold higher affinity than H2A-H2B in vitro, and H3K56Ac increases the binding affinity of CAF-1 toward H3-H4 2-fold. (elsevierpure.com)
  • Recombinant Histone H3S10ph (EPL) has been generated using the patented Expressed Protein Ligation (EPL) technology. (activemotif.com)
  • The ligation reaction maintains the native histone bonds, and the resulting recombinant protein more closely mimics natural histones. (activemotif.com)
  • The recombinant histone is >98% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. (activemotif.com)
  • The molecular weight of the recombinant histone is 15,306 Daltons. (activemotif.com)
  • Recombinant Histone H3 phospho Ser10 tested by SDS-PAGE gel. (activemotif.com)
  • SDS-PAGE analysis of 2 µg Recombinant Histone H3 phospho Ser10 (lane 1) and 1 µg Recombinant Histone H3 phospho Ser10 (lane 2). (activemotif.com)
  • SDS-PAGE gel of Recombinant Histone H3 phospho Ser10. (activemotif.com)
Tetramer4
  • Surprisingly, H3-H4 exists as a dimer rather than as a canonical tetramer at mid-to-low nanomolar concentrations. (elsevierpure.com)
  • A single CAF-1 molecule binds a cross-linked (H3-H4) 2 tetramer, or two H3-H4 dimers that contain mutations at the (H3-H4) 2 tetramerization interface. (elsevierpure.com)
  • These results suggest that CAF-1 binds to two H3-H4 dimers in a manner that promotes formation of a (H3-H4) 2 tetramer. (elsevierpure.com)
  • Histone H4 combines with Histone H3 to form the H3/H4 tetramer. (neb.com)
Antibody1
  • This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70°C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. (novusbio.com)
Demethylase1
  • For detailed information about [histone H3]-dimethyl-L-lysine9 demethylase, go to the full flat file . (brenda-enzymes.org)
Highly conserved1
  • These are denoted as Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.4 (H3T), Histone H3.5, Histone H3.X and Histone H3.Y but have highly conserved sequences differing only by a few amino acids. (wikipedia.org)
Phospho1
  • A análise do ciclo celular com 5-ethynyl-2'-desoxyuridina (EdU) e rotulagem phospho-histone H3 (pH3) é um procedimento de várias etapas que pode exigir uma otimização extensiva. (jove.com)
Lys91
  • Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Butyryl/Crotonyl-Histone H3 (Lys9) (E9N8C) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP ® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. (cellsignal.com)
Repressive1
Linker1
  • Additionally, Histone H3 (1-20) K4Me3, K9Ac-GG-[Lys(5-FAM)] has a C-terminal GKK linker labelled with 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag. (crbdiscovery.com)
Residues1
Mammalian2
  • Histone H3.3 has been found to play an important role in maintaining genome integrity during mammalian development. (wikipedia.org)
  • G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for mouse embryogenesis. (novusbio.com)
Subunit1
  • Thompson, M. (2009) Polybromo-1: the chromatin targeting subunit of the PBAF complex. (mtu.edu)
Transcription1
  • A total of 99 anterior lens capsules (ALCs) of patients with ARC of various nuclear grades, ultraviolet models of ALCs, and two human lens epithelial cell lines (FHL-124 and SRA01/04) were used, and the expression of histone H3, CRYAA, GLB1, and p53 were detected by immunoblotting and reverse transcription and real time-quantitative polymerase chain reaction. (techscience.com)
Modification1
  • Histone H3-K9 (H3-K9) modification status was also closely related to MUC17 expression. (nih.gov)
Epigenetics2
  • Clinical epigenetics 2023 8 15 (1): 129. (cdc.gov)
  • Epigenetics 2023 6 18 (1): 2180585. (cdc.gov)
Inhibitor2
  • SJ901 is a multi-arm, phase 1/2 dose-escalation/dose-finding and early efficacy study of the brain-penetrant inhibitor, mirdametinib in patients with pediatric low-grade glioma (pLGG). (stjude.org)
  • Silencing of the SAC during metaphase activates the ubiquitin ligase APC/C (anaphase-promoting complex, also known as the cyclosome) and results in the proteasomal destruction of the separase inhibitor securin 1 . (nature.com)
Chromatin organization1
Species1
  • 1 species of vertebrate of pooled ticks were separated by 1-dimensional sodium reservoirs. (cdc.gov)
Absorbance2
  • The protein concentration (1 mg/ml, 89 µM) is calculated using the molar extinction coefficient for Histone H4 (5120) and its absorbance at 280 nm (3,4). (neb.com)
  • Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. (activemotif.com)
H3.21
  • Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. (activemotif.com)
Phosphorylation1
Methylates1
  • while DNA methyl transferase 1 (Dnmt1) methylates cytosine forming 5 methyl cytosine (5mC), concomitant activation of Tet2 hydroxymethylates 5mC, forming 5 hydroxymethyl cytosine (5hmC). (nature.com)
Regulation1
  • p62/Sequestosome 1 (p62) is a stress-induced protein that is involved in several different intracellular pathways, including regulation of aspects of protein degradation. (molvis.org)
Human3
  • The enriched DNA was quantified by real-time PCR, using human GAPDH exon 1 primers, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP ® Human α Satellite Repeat Primers #4486. (cellsignal.com)
  • An E. coli strain that carries a plasmid encoding the cloned human histone H4 gene, HIST2H4. (neb.com)
  • Fig. 1: MAD2-dependent binding of human SGO2 to separase. (nature.com)
Primary Antibodies1
  • Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)
Synthesis1
  • GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. (nih.gov)
Thermodynamic2
  • These results provide a quantitative thermodynamic explanation for the specific H3-H4 histone chaperone activity of CAF-1. (elsevierpure.com)
  • Thompson, M. (2009) Thermodynamic and kinetic analysis of bromodomain-histone interactions. (mtu.edu)
20191
Yeast1
  • Methyl-histone binding analysis of the yeast double chromodomain protein Chd1. (mtu.edu)
20182
HATs1
Homolog1
  • fem-1 homolog C (FEM1) and UbiA prenyltransferase domain-containing protein 1 (UbiA). (mdpi.com)