Histological technique. Medical search. Frequent questions

The preparation of the sample is much more rapid than with traditional histology technique (around 10 minutes vs 16 hours). (wikipedia.org)
Quantitative histology was used to evaluate 8 biomarkers of photoaged skin, and associations between grades of dermal elastosis and each of the other 7 biomarkers were analysed using ordinal logistic regression models with proportional odds assumption, using histological grades of elastosis as the outcome. (karger.com)
Histology services prepare samples for analysis using a variety of techniques for subsequent examination. (otago.ac.nz)
The unit provides a well-equipped laboratory for performing histological analysis for diagnostic and research purposes, as well as staff with the necessary expertise to offer advice and guidance on histology and cytological techniques. (otago.ac.nz)
With our specialised equipment and expert team of scientists and technicians, we have the flexibility to tailor our histology techniques to suit any research project. (otago.ac.nz)

Other techniques involve dehydration in alcohols and infiltration with paraffin, or some similar agent - a process called embedding. (columbia.edu)
Our main aim was to investigate if the paraffin technique can be a viable alternative. (elsevierpure.com)
Using Mayer's hematoxylin stained sections of 8 mu m thickness, good agreement of ages was obtained from both techniques, with more preparations classified as "good quality" for the paraffin technique. (elsevierpure.com)
Mayer's hematoxylin provided the best contrast of the GLGs when using the paraffin technique. (elsevierpure.com)
We conclude that the paraffin technique is viable and represents a cost-effective alternative to a cryostat microtome when preparing cetacean teeth for age determination. (elsevierpure.com)
The Core offers high-quality mouse dissection, tissue processing, slide preparation from paraffin-embedded and frozen tissues, routine and specialized histological staining, and expert interpretation of slides. (harvard.edu)
HS-300 High-quality ethanol is essential for excellent results in paraffin techniques and related methods. (nationaldiagnostics.com)

This study aims to compare histological and clinical outcomes of two different surgical techniques to seal the post-extractive site with the use of autologous demineralized extracted tooth as graft material. (thieme-connect.com)
Objective: To determine the technical feasibility and clinical outcomes of laparoscopic anterior resection using combined single-port and endoluminal technique. (sages.org)

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It usually requires an electron microscope and biochemistry techniques. (fact-archive.com)
In addition, the tissue can be subsequently examined with histological techniques using light microscopy or electron microscopy. (dtu.dk)
This technique provides information that is difficult to acquire from histological samples without the use of electron microscopy techniques, which may provide higher sensitivity and resolution, but are vastly more resource-intensive and time-consuming than light microscopy. (cdc.gov)
We show that a variety of histological procedures can be carried out (cyto- and myeloarchitectonics, histochemistry, immunohistochemistry, intracellular cell injection, and electron microscopy). (lu.se)
This procedure resulted in similar morphological features to those obtained by intravascular perfusion in experimental animals, provided that the postmortem interval was under 10 h for several of the techniques used and under 4 h in the case of intracellular injections and electron microscopy. (lu.se)

The reason for these microtomes being so commonly used in the laboratory and histological laboratories in general, is that these microtomes are able to produce truly flat sections, unlike rocking microtomes for example which often produce concave sections from the tissue. (histologicaltechniques.com)
How to prepare histological sections of non-decalcified tissue with metal implants? (bvsalud.org)
This technique produces histological slides of excellent quality, with no damage to the bone-implant interface, which allows detailed observation of tissue architecture, cell types present and fluorochromes. (bvsalud.org)
In group A, the pedunculate tissue was used, while in group B ice cone technique. (thieme-connect.com)
The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. (harvard.edu)
The use of National Diagnostics' Histological Grade Ethanol in tandem with National Diagnostics Histo-Clear-our low toxicity clearing agent (HS-200)-ensures excellent tissue processing results. (nationaldiagnostics.com)
For this technique, histological tissue samples are prepared as they would be for light-based microscopy. (cdc.gov)
From a biomechanical standpoint, several factors appear to be important in determining the success of implant placement as they relate to technique: stiffness of the tissue-implant interface, the quality of the supporting tissues, and implant diameter, particularly as it applies to short implants. (medscape.com)
With volumetric imaging of soft tissue samples, virtual slicing and 3D visualization bring histological studies to the next level. (lu.se)

While some techniques only perform the task of cell nuclei segmentation and quantification, techniques that are capable of further detecting and classifying abnormal tumors (cell nuclei) that cause various types of cancer have also been proposed. (hindawi.com)
It is also an important tool of anatomical pathology since accurate diagnosis of tumors and other diseases usually requires histological examination of samples. (fact-archive.com)
E-mail: [email protected] according to molecular and histological features of tumors. (bvsalud.org)

The PCR assay developed here is more sensitive and less ambiguous than standard histological and cytological techniques. (int-res.com)
South Africa) with subsequent histological investigations and attempt to identify predictors of pathology relevant to the clinical management of women with a cytological diagnosis of AGC. (bvsalud.org)

Bone specimens were obtained by trephine bur to achieve histological and histomorphometrical analysis to evaluate the residual grafted material, the percentages of newly-formed bone and marrow spaces. (unict.it)
Histological analysis shows that at low magnification trabecular mature bone was present in all specimens without any pathological inflammatory cell infiltrate. (unict.it)
A bone samples were collected in each site after 4 months for histological analysis. (thieme-connect.com)
The histological analysis revealed 46.1 ± 8.07% of bone volume, 9.2 ± 9.46% of residual graft, and 35.2 ± 12.36% of vital bone in group A, while group B shows 41.22 ± 5.88% of bone volume, 7.94 ± 7.54% of residual graft, and 31.7 ± 7.52% new bone. (thieme-connect.com)
Histological analysis of biopsy samples retrieved from both sides will be performed to evaluate the biological features of the bone. (biomedcentral.com)
The segmentation and quantification of cell nuclei are two very significant tasks in the analysis of histological images. (hindawi.com)
The proposed translational computation system aims to integrate clinical insight and computational analysis by identifying and segmenting objects of interest within histological images. (hindawi.com)
This puts a limit on how much benefit to receive from a research facility like MAX IV because data is not utilized as much as it could with better analysis techniques. (dtu.dk)
The histological analysis revealed viable neurons close to the electrode contacts, minor microglial and astrocytic reactions and no major changes in the vasculature, indicating high biocompatibility. (lu.se)
Materials and Methods: Detailed postmortem was carried out using lettules techniques and bits taken for histological analysis to ascertain the cause of death. (who.int)

Learn to safely manage chemicals, follow procedures, and operate basic equipment with our Certificate IV in Laboratory Techniques. (edu.au)

If some water is remaining in the sample, the embedding process does not properly reach all the sample regions, so that the technique yields poor-quality sections and tissues. (uvigo.es)
Chun P, Yu S, Sui H et al (2015) The use of P45 plastination technique to study the distribution of preseptal and preaponeurotic fat tissues in asian eyelids. (springer.com)
Histological examination of tissues starts with surgery , biopsy or autopsy . (fact-archive.com)

The intraoperative consultation is the name given to the whole intervention by the pathologist, which includes not only frozen section but also gross evaluation of the specimen, examination of cytology preparations taken on the specimen (e.g. touch imprints), and aliquoting of the specimen for special studies (e.g. molecular pathology techniques, flow cytometry). (wikipedia.org)
Histological examination revealed T2N0 moderately differentiated adenocarinoma with 11 lymph nodes harvested. (sages.org)

The objective the student research are to employ advanced MRI techniques and histological methods to better understand the involvement of white matter in animal models of leukoencephalopathies and in pediatric patients with this group of diseases. (kennedykrieger.org)
METHODS: Endocytoscopic images and histological images of duodenal lesions from 70 patients were retrospectively collected. (bvsalud.org)

Objective Post-extractive socket grafting techniques reduce alveolar ridge dimensional changes. (thieme-connect.com)

Histological changes in respiratory and gastrointestinal epithelium have been observed from both inhalation and oral exposures, respectively. (cdc.gov)

To explore the clinical application of orbital fat fascia flap in the correction of sunken upper eyelid in Asian blepharoplasty based on anatomical and histological research. (springer.com)
Based on the anatomical and histological characteristics of orbital fat, 36 patients with mild to moderate sunken upper eyelids were corrected by transposition of orbital fat fascia flap with preservation of fascia vascular pedicle. (springer.com)

ABSTRACT: The development of diagnostic assays more sensitive and specific than traditional histological techniques is important for the management of bonamiasis in flat oysters Ostrea edulis . (int-res.com)

Moreover, correlations in DSMs and glands between endocytoscopy and histological images were analyzed. (bvsalud.org)

A number of embedding substances are available to get specific section thickness and for performing particular techniques. (uvigo.es)

This experiment aimed to investigate the effects of cadmium (Cd) on Semi-lethal concentration (LC 50 ), histological characteristics and liver DNA damage of juvenile largemouth bass. (researchsquare.com)

Conclusion: Laparoscopic anterior resection using this combined single-port and endoluminal technique is feasible for small lesions in the sigmoid colon or upper rectum. (sages.org)

Dermal elastosis is considered the histological 'gold standard' for evaluation of skin photoaging, but the relation of the level of dermal elastosis to other histological indicators of photoaging is not clear. (karger.com)
To investigate how various proposed histological measures of photoaging compare with the level of dermal elastosis. (karger.com)

A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)

Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after formalin fixation, and pathologist Dr William Welch, also at Hopkins, experimented with Cullen's procedure but without clinical consequences. (wikipedia.org)

Transbronchial needle aspiration (TBNA) is a safe and useful sampling technique for the diagnosis of mediastinal adenopathies/masses, but its accuracy seems to be influenced by selected clinical and procedural aspects. (karger.com)

PPC-SRµCT techniques provide important complementary information to the conventional histological method for characterizing the microstructure of the PPTI, and may facilitate in investigations of the repair mechanism of the PPTI after injury and in evaluating the efficacy of a different therapy. (iucr.org)
The use of intravascular fixation of the brain inside the skull provides a fixed whole human brain, perfectly fitted to the skull, with negligible deformation compared to conventional techniques. (lu.se)

Identification of metal oxide nanoparticle s in histological samples by enhanced darkfield microscopy and hyperspectral mapping. (cdc.gov)
The need for tools and techniques that aid in the identification, localization, and characterization of nanoscale materials in biological samples is on the rise. (cdc.gov)
Enhanced darkfield microscopy complemented with a hyperspectral imaging system may provide a solution to this bottleneck by enabling rapid and less expensive characterization of nanoparticle s in histological samples. (cdc.gov)

Observe the histological structure of the orbital fat and its fascia vascularity through cadaver anatomy and histological sections of 10 cadavers. (springer.com)

Results No significant clinical differences among the different sealing techniques were observed. (thieme-connect.com)
Histological results after maxillary sinus augmentation with Straumann® BoneCeramic, Bio-Oss®, Puros®, and autologous bone. (thieme-connect.com)
Results: This technique was attempted in an 82 with an ulcerative tumor growth over the sigmoid colon. (sages.org)
Adapting to robust automated image processing techniques for primary tasks such as cell nuclei segmentation and quantification will not only prove to be time efficient for pathologists, but these techniques will also be capable of producing consistent results. (hindawi.com)
Also techniques and results of reducing speckle contrast by averaging multiple B-scans will be presented. (spie.org)

This technique has greatly increased the ability to identify categories of cells under a microscope. (fact-archive.com)

Each patient gives a written consent for publication of personal surgical and histological data. (thieme-connect.com)

Available at: https://pathologika.com/histological-techniques-perls/ [Acedido: data]. (pathologika.com)
however, the interesting data obtained have shown how these techniques, mixed with the autologous dentin derived graft material, seem to promote bone regeneration and reduce physiological bone resorption during alveolar socket preservation treatments. (thieme-connect.com)

However, as the result of recent advancements in microscopic imaging technology and computational image processing techniques [ 1 ], there has been significant growth of research towards translational computational systems that can detect, analyze, classify, and quantify cell nuclei from microscopic images. (hindawi.com)
Digital cameras are increasingly used to capture histological images. (fact-archive.com)

Histological grading was highly predictive of clinical response, especially in stage IE(1) patients, with complete remissions in 10/12 tumours with purely low-grade (type A) morphology and 1/8 tumours with increased numbers of blasts (type B) (p=0.0046) and was related to the expression of costimulatory markers (p=0.0061). (nih.gov)
Patients were randomly subdivided into two equal groups according to the site closure technique. (thieme-connect.com)
Major predictors in an unselected population, as well as in patients with suspected/known lung cancer, included lymph node size (short axis length ≥2 cm), presence of abnormal endoscopic findings, subcarinal and right paratracheal location, and the use of histological needle by an experienced bronchoscopist. (karger.com)

Other advanced techniques include in situ hybridization to identify specific DNA or RNA molecules, and confocal microscopy. (fact-archive.com)

We considered whether estimated age would be affected by preparation technique, staining method, and section thickness, while controlling for effects of species, body length, and sex. (elsevierpure.com)

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Although the staining method had significant effects on the estimated age using both techniques, the variability of GLG counts was small and appeared to be similar for both techniques. (elsevierpure.com)

The method of processing is usually done with the bread loafing technique. (wikipedia.org)

The clinical and histological evidences show that MgHA/collagen-based scaffold can be used, successfully, for sinus augmentation procedures. (unict.it)

Afin d'étudier l'effet de l'adoucissement de l'eau du robinet (extraction du calcium) sur les colorations à l'hématoxyline et à l'éosine, cinq (5) séries de lames issues de 30 blocs de tissus pathologiques humains différents inclus en paraffine ont été préparées de la même manière, sauf pour le lavage qui a été effectué avec cinq types d'eau différents. (who.int)

The symposium will cover applications of advanced x-ray micro-tomography to complement standard microscopy techniques. (lu.se)

Histology5

Paraffin7

Clinical outcomes2

Electron17

Tissue9

Tumors3

Cytological2

Analysis10

Laboratory1

Tissues3

Examination2

Methods2

Alveolar ridge1

Epithelium1

Anatomical2

Diagnostic1

Glands1

Embedding1

Characteristics1

Lesions1

Dermal2

Approaches1

Frozen1

Diagnosis1

Conventional2

Samples3

Anatomy1

Results5

Microscope1

Publication1

Data2

Images2

Patients3

Situ1

Section thickness1

Light1

Significant1

Method1

Show1

Types1

Standard1