We analyzed standard reference RNA samples and RNA samples obtained from human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs). (biomedcentral.com)
Organoids1
Site-directed ncAA mutagenesis will open a wide range of applications to probe and manipulate proteins in brain organoids and other hiPSC-derived cell types and complex tissue models. (nih.gov)
HiPSCs7
Post-thawing viability of HiPSCs is typically higher than 70%, and HiPSC have demonstrated coherent pluripotent behavior over more than 60 passages. (cellapplications.com)
Although it is in theory possible to propagate HiPSCs indefinitely, HiPSC subculturing over passages higher than is usually not recommended, as the chances of karyotypic abnormalities increase. (cellapplications.com)
While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
Our set-up enables automated, user-independent expansion of hiPSCs under fully defined conditions, and could be exploited to generate a large number of hiPSC lines for disease modeling, and drug screening at industrial scale, and quality. (frontiersin.org)
However, to realize the full potential of hiPSCs for disease modeling and drug screening, several challenges still need to be overcome, as there is the high variability between hiPSC lines, the risk of accumulating genetic aberrations when culturing hiPSCs, and the lack of standardized procedures for hiPSC generation as such (reviewed by Volpato and Webber, 2020 ). (frontiersin.org)
This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs. (addgene.org)
To minimize the differences between species and fast screening, hiPSC-derived neurons and a microelectrode array (MEA) that could simultaneously measure the action potentials of the neuronal networks were used. (ijstemcell.com)
Also, this is the first paper that evaluates various illicit drugs and NPS using MEA-based neurotoxicity assessment using hiPSC-derived neurons. (ijstemcell.com)
Our study expanded the scope of application of neurotoxicity assessment using hiPSC-derived neurons to NPS, and accumulated evaluation data of various toxic substances for hiPSC-derived neurons. (ijstemcell.com)
Hypertrophic cardiomyopathy1
Chapter 5 focused on the familial hypertrophic cardiomyopathy (FHC)-associated mutations found in patients, I79N TNNT2, which I generated using CRISPR/Cas9 in hiPSC-CMs. (sfu.ca)
Cells6
This led us to question whether excessive Ca V 3 channels, retained mistakenly in engineered human-induced pluripotent stem cell-derived islet (hiPSC-islet) cells, act as an obstacle to hiPSC-islet maturation. (mdpi.com)
FUJIFILM Cellular Dynamics' expertise in hiPSC development and manufacture, enables standardized and homogenous batches of hiPSC-derived cells, ensuring reproducible large-scale screens and long-term projects. (fujifilm.com)
Our new integrated hiPSC offering will enable scientists and researchers to approach even more drug discovery and early development projects with biologically-relevant cells, which will have a profound impact on the way research is conducted. (fujifilm.com)
We tested the growth and differentiation capacity of human induced pluripotent stem cells (hiPSC)-derived RGCs when grown in interface with such photostimulated device, and found that they were significantly increased. (tmu.edu.tw)
The field of 3D cell culture systems using diverse hiPSC-derived cells is growing and gets steadily advanced. (jneurology.com)
Spatial Environment Affects HNF4A Mutation-Specific Proteome Signatures and Cellular Morphology in hiPSC-Derived β-Like Cells. (uib.no)
Differentiation2
Unguided differentiation confirms HiPSC ability to generate cell derivatives of tissues arising from the three embryonic layers. (cellapplications.com)
However, it is still unclear to what extent hiPSC may be capable of recapitulating in vitro the cellular and molecular features of the native retina, especially regarding photoreceptor differentiation and functional maturation. (nature.com)
Retinal2
Here we report that hiPSC can, in a highly autonomous manner, recapitulate spatiotemporally each of the main steps of retinal development observed in vivo and form three-dimensional retinal cups that contain all major retinal cell types arranged in their proper layers. (nature.com)
Moreover, the photoreceptors in our hiPSC-derived retinal tissue achieve advanced maturation, showing the beginning of outer-segment disc formation and photosensitivity. (nature.com)
Maturation1
We addressed this question by using the anterior chamber of the eye (ACE) of immunodeficient mice as a site for recapitulation of in vivo hiPSC-islet maturation in combination with intravitreal drug infusion, intravital microimaging, measurements of cytoplasmic-free Ca 2+ concentration ([Ca 2+ ] i ) and patch clamp analysis. (mdpi.com)
Cell7
HiPSC differentiate into cell derivatives from the 3 embryonic layers: Neuronal marker beta III tubulin (TUJ1), Smooth Muscle Actin (SMA) and Hepatocyte Nuclear Factor 3 Beta (HNF3b). (cellapplications.com)
Unlike the isogenic control cell line, irregular voltage- and Ca2+ transients and arrhythmic activities were observed in the presence higher rates of stimulation or β-adrenergic agonists in monolayer of R37C+/- TNNI1 hiPSC-CMs. (sfu.ca)
Axxam S.p.A., a leading provider of integrated drug discovery services across life sciences industries, and FUJIFILM Cellular Dynamics, Inc., a leading developer and manufacturer of human induced pluripotent stem cell (hiPSC) models and therapies, announce a strategic alliance to improve the drug discovery process. (fujifilm.com)
The pre-clinical stage company is using its expertise in hiPSC technologies to develop a robust pipeline of cell therapeutics candidates. (fujifilm.com)
Here, we review the latest improvements of 3D hiPSC-based cell culture systems in the field of neuroscience. (jneurology.com)
Human induced pluripotent stem cell (hiPSC) technology has revolutionized studies on human biology. (nih.gov)
hiPSC (human induced pluripotent stem cell) are a source of potential cellular immunotherapies. (aicompanies.com)
Vitro1
By producing LQTS-hiPSC-CMs, we assessed the impact of different drugs on action potential duration (APD), which is used as an in vitro surrogate for QT interval. (worktribe.com)
Human4
Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured. (lu.se)
Human primary cultures exhibited features in between hiPSC-derived and rat primary cultures, although traits from the former predominated. (lu.se)
Here, we describe the StemCellFactory, an automated, modular platform covering the entire process of hiPSC production, ranging from adult human fibroblast expansion, Sendai virus-based reprogramming to automated isolation, and parallel expansion of hiPSC clones. (frontiersin.org)
Therefore, the hiPSC technology is an essential tool to develop human brain-like models. (jneurology.com)
Sendai1
Our HiPSC are generated with the RNA-based Sendai virus to deliver reprogramming factors to donor skin fibroblasts. (cellapplications.com)
Cultures3
We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. (lu.se)
Our study demonstrates that hiPSC-derived cultures are excellent models to investigate development in neuronal assemblies, a hallmark for applications that monitor alterations caused by damage or neurodegeneration. (lu.se)
We found that BD hiPSC-RPE cultures possessed increased protein oxidation, decreased free-ubiquitin levels, and altered rates of exosome secretion, consistent with altered POS processing. (wisc.edu)
Therapeutic1
For its partners, FCDI utilizes its hiPSC platform to advance the progress of therapeutic candidates in the clinic and provides contract development and manufacturing (CDMO) services. (fujifilm.com)
Model4
In sum, hiPSC-CMs have been successfully used to model a growing number of arrhythmogenic disorders, thereby enabling prediction of high-risk populations' susceptibilities to drug-induced cardiotoxicity as a form of personalized medicine. (sfu.ca)
GFAP Mutations in Astrocytes Impair Oligodendrocyte Progenitor Proliferation and Myelination in an hiPSC Model of Alexander Disease. (nygenome.org)
Consistent with this concept, a recently established hiPSC-RPE model of inherited macular degeneration, Best disease (BD), displayed reduced rates of POS breakdown. (wisc.edu)
The utility of hiPSC-RPE for VPA testing was further evident following examination of its efficacy and metabolism in a complementary canine disease model. (wisc.edu)
Mechanisms3
In particular, hiPSC-based models provide a powerful tool to identify genetic risk factors, and to study cellular and molecular mechanisms that contribute to the pathogenesis of a disease. (frontiersin.org)
In this study, we generated hiPSC -CMs and murine models to elucidate the mechanisms underlying CHK1 inhibition combined with gemcitabine -induced cardiotoxicity and identify potential targets for cardioprotection. (bvsalud.org)
Using hiPSC-CMs to Examine Mechanisms of Catecholaminergic Polymorphic Ventricular Tachycardia. (rndsystems.com)
Mice1
SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice. (bvsalud.org)
Disease2
This success brings us one step closer to the anticipated use of hiPSC for disease modelling and open possibilities for future therapies. (nature.com)
Besides disease modeling and drug screening, hiPSC-CMs have emerged as a powerful platform for studying the cardiotoxicity of chemotherapeutic agents. (sfu.ca)
Assessment1
The alliance will provide drug discovery researchers and scientists access to an integrated platform of hiPSC-based assays, through the application of the most advanced drug discovery technologies to enable target assessment, High-Throughput Screening (HTS), and High-Content Screening. (fujifilm.com)
Study1
This study demonstrates the utility of LQTS-hiPSC-CMs in evaluating whether drugs can shorten APD and, importantly, shows that PPAR? (worktribe.com)
Drug1
Today, we are pleased to offer even more hiPSC-based drug discovery solutions through our alliance with Axxam. (fujifilm.com)
Technology1
Specifically, I generated R37C+/- TNNI1 hiPSC-CMs using the genome-editing technology CRISPR/Cas9 and monitored voltage- and Ca2+ transients through optical mapping. (sfu.ca)
Development of new1
As part of the collaboration, Axxam will provide its deep understanding of the screening process, data analysis and hit selection criteria, for the development of new hiPSC-based applications. (fujifilm.com)
Lines1
When analyzing four closely related hiPSC-CM lines, we show that both AmpliSeq and RNA-seq capture similar global gene expression patterns consistent with known sources of variations. (biomedcentral.com)
Early1
The I-V relationships of the postsynaptic currents (hIPSC and GDPSC) had distinct characteristics: the hIPSC and the early GDPSC showed outward rectification, whereas the late GDPSC was reduced with positive voltage steps to zero or beyond (inward rectification), but often no clear reversal was seen. (jneurosci.org)
Field1
As the leading expert in the hiPSC field, FUJIFILM Cellular Dynamics has high-quality standards, which are fundamental for conducting robust discovery projects. (fujifilm.com)
Control1
Unlike control hiPSC-CMs, the mutant hiPSC-CMs developed irregular and arrhythmogenic voltage- and Ca2+ transients at high stimulation rates and in the presence of β-adrenergic agonists. (sfu.ca)
Presence1
right panels) BD-2 hiPSC-RPE in the presence (lower panels) or absence (upper panels) of daily POS feeding for 1.5 months. (wisc.edu)