Highly soluble highly immunogenic carrier protein carrier proteins. Medical search. Frequent questions

Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH. (fishersci.com)
Thermo Scientific™ Imject Maleimide-Activated mcKLH is sulfhydryl-reactive immunogenic mariculture keyhole limpet hemocyanin (KLH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens. (fishersci.com)
Chicken are good substitute for rabbits when using mammalian antigens especially those that are highly conserved. (biologicscorp.com)
Although the assembled VLPs do not have S protein spikes, they could be developed as formulations that can improve the immunogenicity of vaccines including S antigens, and further could be used as platforms that can carry S antigens of concern for various mutations. (nature.com)
Indeed, hemocyanins have a multivalent nature as highly mannosylated antigens. (exeter.ac.uk)
Adjuvants are generally used with soluble protein antigens to increase antibody titers and induce a prolonged response with accompanying memory. (wikidoc.org)
Most complex protein antigens induce multiple B-cell clones during the immune response, thus, the response is polyclonal. (wikidoc.org)
Immune responses to non-protein antigens are generally poorly or enhanced by adjuvants and there is no system memory. (wikidoc.org)
Proteins that activated immunity are potential antigens for immunization and the multi-omics phylum-spanning prioritization database that was created is a valuable resource for identifying target proteins in a wide array of different parasitic nematodes. (frontiersin.org)
Conjugate vaccines in which polysaccharide antigens are covalently linked to carrier proteins belong to the most effective and safest vaccines against bacterial pathogens. (biomedcentral.com)
Two different periplasmic carrier proteins, AcrA from C. jejuni and a toxoid form of Pseudomonas aeruginosa exotoxin were glycosylated with Shigella O antigens in E. coli . (biomedcentral.com)
Neutralizing activities of antisera to poxvirus soluble antigens. (microbiologyresearch.org)
Multimodal immunogenic cell death (ICD) together with autophagy often induced by OVs not only presents potent danger signals to dendritic cells but also efficiently cross-present tumor-associated antigens from cancer cells to dendritic cells to T cells to induce adaptive antitumor immunity. (biomedcentral.com)

Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
Recent advances in drug design have led to the development of several classes of novel therapeutic macromolecules including peptides, proteins, monoclonal antibodies, immunotoxins, lysozymes, plasmid DNA, antisense oligodeoxynucleotides, and short interfering RNA. (usc.edu)
The development of new varieties lacking immunogenic peptides is one of the strategies currently investigated to address the CD problem. (biomedcentral.com)

A series of studies have revealed that exosomes-mediated intercellular transfer of proteins, nucleic acids, and metabolites plays a crucial role in the initiation and progression of HCC, hypoxia and angiogenesis, chemotherapy sensitivity, and cell death mode and regulates the immune microenvironment. (hindawi.com)
Regardless of the type or the physiological or pathological state of the originating cell, exosomes share common features as enriched with nucleic acids, lipids, and proteins [ 4 ]. (hindawi.com)
In this seminar, I will discuss our research efforts to develop "intelligent" pH-sensitive, membrane-destabilizing, and degradable polymeric carriers that can effectively deliver therapeutic nucleic acids past the endosomal membrane and into the cytoplasm of cancer cells to successfully suppress the expression of targeted genes. (usc.edu)
As such as proteins, lipids, glycans, nucleic acids, and isoporous ultrafiltration membrane they can apparently combination of that. (2medicalcare.com)

Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
The AFFITOPE® formulation, the basis of SAIT, is administered to patients via a subcutaneous injection, which stimulates the generation of antibodies against the target proteins. (affiris.com)

VLPs are highly immunogenic and can induce elevated titers of neutralizing antibodies, even without adjuvants, thanks to the maintenance of the native conformation of viral proteins presenting repetitive epitopes to the host immune system cells. (nature.com)
This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
A closed prefusion conformation or an open (non-prefusion) conformational state of a protein vaccine candidate molecule can determine if it effectively elucidates a desired immunity. (bvsalud.org)
Through the bound monoclonal antibody protein vaccine peak shift in the size-exclusion chromatography profile, this method determines the percent closed (prefusion) conformation present in a sample. (bvsalud.org)
We obtained recombinant MVA expressing the unmodified full-length SARS-CoV-2 spike (S) protein containing the D614G amino-acid substitution (MVA-Sdg) and a version expressing a modified S protein containing amino-acid substitutions designed to stabilize the protein a in a pre-fusion conformation (MVA-Spf). (bvsalud.org)
It is of fundamental importance in determining PROTEIN CONFORMATION. (lookformedical.com)

Strategies, which have been designed to overcome this lack of immunogenicity in infants, include the linking of the polysaccharide to large immunogenic proteins, which provide bystander T-cell help and which induce immunological memory against the polysaccharide antigen to which it is conjugated. (allindianpatents.com)

Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. (fishersci.com)
Among gluten proteins, α-gliadins display the strongest immunogenicity with four main T-cell stimulatory epitopes. (biomedcentral.com)
barley ( Hordeum vulgare L.) and rye ( Secale cereale L.). Oat ( Avena sativa L.) also displays a small fraction of prolamin storage proteins, called avenins, which do not contain any CD-related epitopes [ 3 ]. (biomedcentral.com)
Using its AFFITOME technology, AFFiRiS develops amino acid sequences that mimic the epitopes of self-proteins, which are modified by mutating the original amino acid sequence. (affiris.com)

These protein subunits can assemble into virus particles without viral RNA genome in mammalian cells to study virus-cell entry 2 and to develop vaccines 3 . (nature.com)
represent the only wall component and can be so closely One of the great challenges for nano(bio)technology is associated with the plasma membrane that a hydrophobic the creation of supramolecular materials in which the domain of the constituent subunits is actually integrated into constituent units are highly regular molecular nanostructures. (2medicalcare.com)
In most prokaryotic organisms S-layers surface layers (S-layers) composed of identical protein- have to be considered as non-conservative structures with the aceous subunits represent unique patterning elements and potential to fulfil a broad spectrum of functions [3, 4, 9]. (2medicalcare.com)
Twenty alpha-amino acids are the subunits which are polymerized to form proteins. (lookformedical.com)

MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)

Over one hundred vaccines using varied approaches including protein subunit, viral vector, RNA, DNA, inactivated virus, attenuated virus, and virus like particles (VLPs) platforms are in clinical trials. (nature.com)
Nowadays, growing scientific interest to DT is associated with possibility of its use in medicine as a carrier protein in conjugate vaccines, a component of immunotoxin drugs, and in anticancer therapy. (org.ua)
After induction glycoconjugates generally appeared later than unglycosylated carrier protein, suggesting that glycosylation was the rate-limiting step for synthesis of conjugate vaccines in E. coli . (biomedcentral.com)
Compared to a clinically relevant prime-boost regimen with soluble vaccines formulated with CpG/alum or 3M-052/alum adjuvants, PNP hydrogel vaccines more rapidly generated higher, broader, and more durable antibody responses. (uw.edu)
These anti-viral vaccines are highly effective in curbing virus infections and onset of cancer. (biomedcentral.com)

In addition to our existing immunoassays, we have developed a unique physicochemical approach using size-exclusion chromatography to assess binding between antibody and the structurally desired antigen protein. (bvsalud.org)
A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. (frontiersin.org)
An antibody-protein array of putative immunogenic proteins was developed from a combined bioinformatic, experimental, and literature-based prioritization of homologous parasite proteins. (frontiersin.org)
Using ELISA, we demonstrated that the recombinant protein specifically interacts with the anti-benzo[a]pyrene monoclonal antibody (mAB B2). (actanaturae.ru)
The roles of these proteins in virus morphogenesis and dissemination, and as targets for neutralizing antibody are reviewed. (microbiologyresearch.org)

codon optimization and soluble protein expression available for antigen production. (biologicscorp.com)
Peptide synthesis: Antigen design and conjugate to an immunogenic carrier protein for immunization. (biologicscorp.com)
Mollusk hemocyanins have biomedical uses as carriers/adjuvants and nonspecific immunostimulants with beneficial clinical outcomes by triggering the production of proinflammatory cytokines in antigen-presenting cells (APCs) and driving immune responses toward type 1 T helper (Th1) polarization. (exeter.ac.uk)

Conversion of this insoluble material involves often slow and complex refolding methods, making protein purification difficult. (justia.com)

To cause sensitization, a chemical must bind to macromolecules (proteins) in the skin. (cdc.gov)

The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. (justia.com)
The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions. (justia.com)
The obtained benzo[a]pyrene immunomimetic peptide could only simulate chemical carcinogens in the frame of the pIII protein. (actanaturae.ru)
As a result, we prepared a recombinant protein composed of the benzo[a]pyrene immunomimetic peptide and pIII-encoding sequences. (actanaturae.ru)
Read more about our Recombumin® portfolio of rAlb products, formulated to maintain the unique properties of natural human albumin to enhance protein & peptide formulation , vaccine stabilization and cell therapy processing . (albumedix.com)

For this purpose, we synthesized carcinogen-protein conjugates and prepared mono- and polyclonal antibodies to benzo[a]pyrene. (actanaturae.ru)

Glycoconjugate synthesis, in particular expression of oligosaccharyltransferase PglB, strongly inhibited growth of E. coli cells after induction, making it necessary to separate biomass growth and recombinant protein expression phases. (biomedcentral.com)

This single-chain 535 amino acid long protein causes the symptoms of acute diphtheria infection. (org.ua)
HLA- em DRB1 /em genotypes, high disease activity, high disability scores, and high levels of acute phase proteins [2]. (exposed-skin-care.net)
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. (rndsystems.com)

Celiac disease (CD) is an autoimmune disorder affecting genetically predisposed individuals whose dietary gluten proteins trigger an inflammatory reaction in the small intestine. (biomedcentral.com)

Moreover, TAMs usually display M2-like phenotypes that exert tumor-promoting role in TME, and promote production of related immunosuppressive factors that trigger immunotherapy resistance, including cytokines, chemokines, growth factors and soluble signaling mediators [ 12 ]. (thno.org)

We have previously shown that hemocyanins are internalized by APCs through receptor-mediated endocytosis with proteins that contain C-type lectin domains, such as mannose receptor (MR). However, the contribution of other innate immune receptors to the proinflammatory signaling pathway triggered by hemocyanins is unknown. (exeter.ac.uk)
DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. (uci.edu)

SARS-CoV-2 is composed of four essential structural proteins: the spike (S) glycoprotein, membrane (M) protein, envelope (E) protein, and nucleocapsid (N) protein, as well as multiple accessory and non-structural proteins 1 . (nature.com)
Ribosomal proteins (green) serve in structural and auxiliary roles. (heresy.is)
Unique properties of chitosan may include polyoxy salt formation capability to form of metal ions chelate, film formation and soluble in different media, viscosity, solution formation and optical structural characteristics (Pillai et al. (ijbnb.com)

Gluten proteins are usually classified into soluble monomeric gliadins and insoluble polymeric glutenins according to their alcohol solubility. (biomedcentral.com)
Chitin is highly insoluble and highly hydrophobic in water and organic solvent than compared to chitosan. (ijbnb.com)

Since S-layers rapidly growing scientific and engineering discipline that are composed of a single protein or glycoprotein species crosses the boundaries of numerous existing fields. (2medicalcare.com)

These amino acid sequences, termed AFFITOPEs®, are coupled to a carrier protein and are formulated with an adjuvant to further enhance the immune response. (affiris.com)

There are 90 known serotypes of pneumococci, and the capsule is the principle virulence determinant for pneumococci, as the capsule not only protects the iimer surface of the bacteria from complement, but is itself poorly immunogenic. (allindianpatents.com)

Despite the additional steps which are required to produce a protein of interest as a fusion protein, rather than directly in its active form, the production of hybrid proteins has been found to overcome a number of problems. (justia.com)
The cleavage reaction is performed by contacting the fusion protein with a proteolytic enzyme under appropriate conditions. (justia.com)
No. 4,743,679 which discloses a process for the production of human epidermal growth factor comprising cleavage of a fusion protein by Staphylococcus aureus V8 protease. (justia.com)

In 1937, Creech and Franks first synthesized conjugates of carcinogens with high-molecular carriers - blood serum proteins. (actanaturae.ru)

ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)

Surface (S)-layer proteins and S-layer fusion proteins incorporating functional sequences, self-assemble into monomolecular lattices on solid supports and on various lipid structures. (2medicalcare.com)

Moreover, a majority of chemotherapeutic drugs are highly hydrophobic and need to be diluted in organic solvents, which cause high toxicity, in order to reach effective therapeutic dose. (oaepublish.com)

Based on these S-layer proteins, supramolecular assemblies can be constructed which are envisaged for label-free detection systems, as affinity matrix, as anti-allergic immuno-therapeutics, as membrane protein-based screening devices, and as drug targeting and delivery systems. (2medicalcare.com)
The compound showing the highest binding affinity to the viral S protein was further characterized by surface plasmon resonance (SPR) towards ACE2 and viral S protein binding domain. (bvsalud.org)

Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. (nature.com)

But that is soluble in hexafluoroacetone, hexafluoroisopropanol, chloroalcohols combined with aqueous mineral acids solution and dimethylacetamide along with 5% of lithium chloride. (ijbnb.com)
Chitosan is soluble in diluted acids solution of glacial acetic acid, formic acid and N-methyl morpholine-N-oxide/H 2 O (Dutta et al. (ijbnb.com)

The present invention relates to a combination of 2 or more S. pneumoniae proteins, their manufacture and use in medicine as a vaccine. (allindianpatents.com)
In yet another related aspect is a method for making the vaccine of the invention by selecting and isolating 2 different S. pneumoniae proteins and mixing both proteins with a pharmaceutically acceptable carrier. (allindianpatents.com)

Controlling the biodistribution of protein- and nanoparticle-based therapeutic formulations remains challenging. (uw.edu)
Apart from carrier biodistribution, various other impediments require proper overcoming. (biomedcentral.com)

Albumin is responsible for maintaining oncotic pressure, plasma pH and distribution of a variety of molecules, making it a very well-studied protein. (albumedix.com)
In a world dominated by RNA, one would expect cosubstrates and carrier molecules to contain some nucleotide moieties also. (heresy.is)

Nanomaterials have demonstrated a wide range of applications and recently, novel biomedical studies are devoted to improving the functionality and effectivity of traditional and unmodified systems, either drug carriers and common scaffolds for tissue engineering or advanced hydrogels for wound healing purposes. (researchgate.net)

Here, we describe further development of an in vivo library selection platform based on self-assembling protein nanoparticles encapsulating their own mRNA genomes (synthetic nucleocapsids or synNCs). (uw.edu)

An immunogenic composition comprising 2 S. pneumoniae recombinant proteins wherein one of the proteins is selected from the Poly Histidine Triad family (PhtX) at a dose of between 1-100 µg and optionally comprising an adjuvant. (allindianpatents.com)

Among most immunosuppressive cells, TAMs, as crucial drivers of immunosuppressive TME, account for the largest proportion of immune cells in the TME (approximately 50% of tumor mass) [ 9 , 10 ], which possess highly heterogeneity and play a complex regulatory role in tumor immunity and immunotherapy due to helping tumor evade immune surveillance [ 11 ]. (thno.org)

Enzymes known to the prior art as "proteolytic enzymes" have been found to be particularly well suited for the cleavage of fusion proteins. (justia.com)

On the other hand, DT represents a very interesting molecular machine which allows using this protein in different bioengineering applications, such as ablation of certain cell types in transgenic mice, development of immunotoxins targeted for specific cell markers and also for delivery of certain proteins into the cytosol. (org.ua)

Expert systems containing information about the relationship between the chemical structure and the ability of chemicals to haptenate proteins are available. (cdc.gov)

This leads to an almost complete arrest of protein synthesis in the cell and its death. (org.ua)
In addition, the exosomes released from the same cell line can be highly homogeneous [ 5 ]. (hindawi.com)
Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)
Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
Extracellular vaccinia virus formation and cell-to-cell virus transmission are prevented by deletion of the gene encoding the 37,000-Dalton outer envelope protein. (microbiologyresearch.org)
SARS-CoV-2 binding and entry in human cells involves interactions of its spike (S) protein with several host cell surface factors, including heparan sulfate proteoglycans (HSPGs), transmembrane protease serine 2 (TMPRSS2), and angiotensin-converting enzyme 2 (ACE2). (bvsalud.org)
Efforts have been made to increase drug carrier targeting efficiency by attaching cell receptor-targeted ligands to the particle surface [ 7 ]. (biomedcentral.com)

It was found that efficiency of glycosylation but not carrier protein expression was highly susceptible to the physiological state at induction. (biomedcentral.com)

It is used in protein biochemistry. (lookformedical.com)

Engineering the drug carrier biointerface can help overcome the main biological barriers and optimize the drug delivery in a more personalized manner. (biomedcentral.com)
Although this is a significant step forward in achieving targeted drug delivery, the drug carrier must have to overcome various biological obstacles throughout the body to reach the specific target of interest. (biomedcentral.com)

The preparation of valuable recombinant (genetically engineered) polypeptides, for example pharmaceutical proteins, relies frequently on techniques which involve the production of these polypeptides as fusion or hybrid proteins. (justia.com)

In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)

Lipase activities of p37, the major envelope protein of vaccinia virus. (microbiologyresearch.org)

As expected, the liposomal virus rendered it immunogenic and harder to fight [86,129]. (researchgate.net)
Seven virus-encoded proteins have been identified that are components of IEV, and five of them are present in CEV or EEV. (microbiologyresearch.org)
Loss of immunogenic properties of vaccinia virus inactivated by formaldehyde. (microbiologyresearch.org)
Sequence analysis, expression, and deletion of a vaccinia virus gene encoding a homolog of profilin, a eukaryotic actin-binding protein. (microbiologyresearch.org)
Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly. (uci.edu)

The structure of the ribosome also suggests an evolutionary path along which RNA enzymes may have been replaced by protein enzymes in evolution. (heresy.is)
However, if the above scenario for the evolutionary replacement of RNA enzymes by protein enzymes is correct, why did a similar replacement not occur with cosubstrates? (heresy.is)

AFFiRiS is a clinical-stage biotechnology company located in Vienna, Austria, with a vision of using the immune system to identify and target human proteins central to the development and progression of chronic diseases, based on its proprietary patented AFFITOME® technology. (affiris.com)

In addition, heterogeneity among patients and tumours make drug resistance a highly challenging event [ 5 ] . (oaepublish.com)

Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis ) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. (frontiersin.org)

In one of the well-known chitosan loaded drug delivery system it disturbs adsorption of fat soluble substances. (ijbnb.com)

Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)

Anatomy8

Organisms19

Diseases1

Chemicals and Drugs138

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes58

Technology, Industry, Agriculture3

Information Science4

Named Groups1

Health Care4

Antigens13

Peptides3

Nucleic acids4

Target proteins2

Conformation6

Bystander1

Epitopes4

Subunits4

Lipids1

Vaccines5

Antibody5

Antigen3

Purification1

Macromolecules1

Peptide5

Antibodies1

Recombinant protein1

Acute3

Inflammatory1

Cytokines1

Mannose receptor3

Structural3

Insoluble2

Glycoprotein1

Amino acid1

Poorly1

Fusion protein3

High molecular1

Intercellular1

Sequences1

Hydrophobic1

Affinity2

Viral1

Acids2

Vaccine2

Biodistribution2

Molecules2

Scaffolds1

Vivo1

Composition1

Immunotherapy1

Cleavage1

Molecular1

Chemicals1

Cell8

Glycosylation1

Biochemistry1

Overcome2

Genetically1

Associate1

Envelope1

Virus5

Enzymes2

Immune system1

Heterogeneity1

Specific1

Drug1

Tissue1