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  • assay
  • The protocol features an optimized in situ proximity ligation assay targeting the inositol 1,4,5-triphosphate receptor/glucose-regulated protein 75/voltage-dependent anion channel/cyclophilin D complex at the mitochondria-associated membrane interface. (jove.com)
  • cytoplasm
  • Furthermore, deletion or substitution of the N-terminal 12 amino acids in DJ-1 resulted in re-localization of E18A, M26I and L166P DJ-1 from mitochondria into the cytoplasm. (jove.com)
  • reagents
  • Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. (elifesciences.org)
  • oxidative stress
  • Remarkably, CERKL (but not the RP R257X mutant) exerts a protective role in cells against oxidative stress, consistent with RP mutations impairing the normal protein function in photoreceptors and thus tilting the balance toward apoptosis. (molvis.org)
  • Overexpression
  • And finally, we studied the effects on cell apoptosis after CERKL overexpression in transiently transfected cultured cells by propidium iodide staining and poly-(ADP-ribose)-polymerase (PARP) caspase-dependent cleavage. (molvis.org)
  • The phenotypes of ecm33, aah3 and gaz2 deletion cells were distinct from each other, and Cis4 overexpression suppressed ?ecm33 phenotypes but did not suppress ?aah3 defects. (jove.com)
  • junctions
  • It is released from the presynaptic terminals of inhibitory neurons within highly specialized intercellular junctions known as synapses, where it binds to GABA A receptors (GABA A Rs) present at the plasma membrane of the synapse-receiving, postsynaptic neurons. (jove.com)
  • Apoptosis
  • Conversely, the Bcl-2 antagonist Bak-BH3 peptide or HA14-1 compound abrogates the antiapoptotic effects of Bcl-2 and enhances apoptosis of 468 cells pretreated with EpoB (to induce mitotic arrest). (aacrjournals.org)
  • In synchronized 468 cells, EpoB is more potent in inducing Bax conformational change and apoptosis at G 2 -M phase compared with G 1 -S phase of the cell cycle. (aacrjournals.org)
  • In this paper, we investigated the role of Bax and Bcl-2 in the novel epothilone B analogue BMS-247550 (EpoB)-induced apoptosis of human breast cancer cells. (aacrjournals.org)
  • Retinitis pigmentosa (RP), a retinal neurodegenerative disorder characterized by apoptosis of photoreceptor cells, is caused by mutations in many different genes. (molvis.org)
  • Retinitis pigmentosa (RP) is an inherited retinal neurodegenerative disorder characterized by the progressive attrition of photoreceptor cells through apoptosis. (molvis.org)
  • To avoid entering apoptosis, cells counteract this ceramide increase by activating enzymatic pathways involved in ceramide clearance. (molvis.org)
  • amino acids
  • Subsequent studies showed that (i) every cell has multiple species of tRNA, each of which is associated with a single specific amino acid, (ii) that there are a matching set of enzymes responsible for linking tRNAs with the correct amino acids, and (iii) that tRNA anticodon sequences form a specific decoding interaction with mRNA codons. (wikipedia.org)
  • recombinant
  • One of these advantages resides in the fact that metalation occurs after protein import into mitochondria, which contains pools of iron, zinc, copper and manganese ions that can be utilized in recombinant metalloprotein metalation reactions. (biomedcentral.com)
  • tissue
  • Here the methods for mouse adrenal gland tissue dissection, chromaffin cell culture, basic cell-attached techniques, and subsequent examples of individual traces measuring singular endocytic event are described. (jove.com)
  • interactions
  • Utilize a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait with Thermo Scientificâ„¢ Pierce Magnetic RNA-Protein Pull-Down Kit. (fishersci.com)
  • Bimolecular fluorescence complementation (BiFC) is an in vivo method to monitor such interactions in plant cells. (jove.com)
  • BiFC is ideally suited to study protein-protein interactions in their natural surroundings within the living cell. (jove.com)
  • Mutants
  • Likewise, DJ-1 with mutations of two cysteines located in the dimer interface, C46S and C53A, and pathogenic mutants, M26I and L166P DJ-1, were found to be localized in mitochondria and not to make homodimers. (jove.com)
  • Notably, green fluorescent protein-tagged Ecm33, which was observed at the cell surface in wild-type cells, mostly localized as intracellular dots that are presumed to be the Golgi and endosomes in membrane-trafficking mutants, including ?apm1, ypt3-i5, and chc1-1 mutants. (jove.com)
  • HeLa
  • Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. (elifesciences.org)
  • Two populations of HeLa cells were grown in leucine-deficient DMEM/10% dialyzed FBS. (pnas.org)
  • reporter
  • An advantage of these model systems is the ease of producing modified embryos in which a particular gene is up or downregulated, labeling a group of cells, or introducing a reporter gene. (jove.com)
  • mechanism
  • The spindle assembly checkpoint (SAC) is a mitotic surveillance mechanism that holds back cells at metaphase until every single chromosome has established a stable bipolar attachment to the mitotic spindle 1 . (jove.com)
  • intracellular
  • As such, several independent triggers of egress have been identified which all converge on the release of intracellular Ca 2+ , a signal that is also critical for host cell invasion 6-8 . (jove.com)
  • fluorescent
  • The fluorescent signal can be monitored either directly in epidermal leaf cells or in single protoplasts, which can be easily isolated from the transformed tobacco leaves. (jove.com)
  • Introduction
  • Here, our lab demonstrates a basic introduction to cell-attached capacitance recordings of single endocytic events in the mouse adrenal chromaffin cell. (jove.com)
  • role
  • We also found that the depletion of transcription factor NAI1, a master regulator for ER body formation, suppressed the formation of ER-aggregates in ermo3 cells, suggesting that NAI1 expression plays an important role in the abnormal aggregation of ER. (jove.com)