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  • mutations
  • From a dominant loss-of-function genetic screen, this study discovered that mutations of a Dbl-family member, the guanine nucleotide exchange factor DRhoGEF2 ( DRhoGEF2 2(l)04291 ), suppressed the PTEN-overexpression eye phenotype. (sdbonline.org)
  • Loss-of-function (lof) mutations in Drosophila Rap1 cause severe morphogenetic abnormalities during embryonic development, while cell proliferation and cell fate determination, processes that rely heavily on regulation by Ras, appear to be unaffected. (genetics.org)
  • Based on the recent crystal structure of the complex of Rap1A with the Ras-binding domain of Raf, mutations were introduced in c-Raf-1 and their effects on Ras/Raf binding affinity in vitro and Ras/Raf regulated gene expression in vivo were analysed. (embl-heidelberg.de)
  • Cdc42
  • Characterisation of the nucleotide exchange factor ITSN1L: evidence for a kinetic discrimination of GEF-stimulated nucleotide release from Cdc42. (genes2cognition.org)
  • The selectivity in nucleotide exchange kinetics for GDP over GTP is even more pronounced when a Cdc42 mutant, F28L, is used, which is characterised by fast intrinsic dissociation of nucleotides. (genes2cognition.org)
  • We suggest a novel model for selective nucleotide exchange residing on a conformational change of Cdc42 upon binding of GTP, which enables effector binding to the Cdc42.GTP complex but, at the same time, excludes efficient modulation by the GEF. (genes2cognition.org)
  • interaction
  • Although the FLVRES motif and Arg βB5 play a dominant role in SH2-mediated phosphopeptide binding, they do not fully account for the specificity of SH2 domain substrate interaction. (asm.org)
  • subtypes
  • These experiments highlight differences in desensitization between iGluR subtypes and the highly specific contribution of the GluN2 subunit to this process. (jneurosci.org)
  • occur
  • These data provide a new insight into the evolution of transposons in the genomes of hematophagous insects and bring additional evidences that lateral exchanges of mobile genetics elements occur frequently in the R. prolixus genome. (biomedcentral.com)
  • molecular
  • When it was found that the antibiotic rapamycin was a potent inhibitor of the proliferation of virtually every eukaryotic cell type examined (e.g., fungi, T cells, and tumor cells) [ 1 , 2 , 3 ], it became clear that the molecular target of rapamycin must be highly conserved and its function critical for cell survival. (mdpi.com)
  • Golgi
  • Ras Signalling on the Endoplasmic Reticulum and the Golgi Nature Cell Biology. (jove.com)