• The NIH Common Fund Protein Capture Program aims to develop over time the capacity to generate a community resource of high quality renewable affinity reagents for all human proteins (see http://nihroadmap.nih.gov/proteincapture ). (nih.gov)
  • This Funding Opportunity Announcement (FOA) is a component of the Protein Capture Program, and is designed to solicit grant applications that would develop and/or improve approaches for obtaining protein affinity reagents at high throughput and low cost. (nih.gov)
  • Protein affinity reagents have been widely used in basic research and clinical applications. (nih.gov)
  • This NIH Common Fund FOA seeks to establish a center or centers to produce renewable, high quality affinity reagents against all human transcription factors. (nih.gov)
  • The resulting set of affinity binding reagents is intended to form a broadly available community resource for researchers with an interest in transcription factors. (nih.gov)
  • However, the scientific application that is the highest priority for this FOA is the use of the reagents in chromatin immunoprecipitation (ChIP) studies. (nih.gov)
  • It is anticipated that awarded applications will propose to create production centers capable of implementing a pipeline that would include acquisition and evaluation of human transcription factor immunogens, production and selection of affinity binding reagents, and the adequate biochemical characterization and validation of the affinity reagents. (nih.gov)
  • At the same time, the production center must maintain a degree of scientific flexibility for example to develop approaches to producing affinity reagents to less tractable transcription factor protein targets as they are identified in the course of production. (nih.gov)
  • The availability of pre-activated supports allows the user to quickly and easily custom synthesize affinity resins without having to handle highly reactive reagents. (sigmaaldrich.com)
  • Memory B cells are marked by longevity, expression of antibodies with high affinity, and ability to generate robust antibody responses upon reencountering pathogens. (umd.edu)
  • Antigen was required for the clonal expansion of antigen-specific memory B cells, the differentiation of mB cells to high affinity IgG secreting plasma cells, and the recall of high affinity antibody responses. (umd.edu)
  • The Human High affinity IgE Receptor Antibody ELISA kit can be used to identify samples from the human species. (elisakit.cc)
  • High affinity IgE Receptor Antibody can also be called FcεRI Ab, Fc epsilon RI Ab, FcεRI Antibody, Fc epsilon RI Antibody. (elisakit.cc)
  • High Affinity K+ transporter HAK5 is a transport protein found on the cell surface membrane of plants under conditions of potassium deprivation. (wikipedia.org)
  • Each plant species has its own HAK5 transporter that is specific to that species and has different levels of affinity to K+. (wikipedia.org)
  • The High Affinity K+ transporter interacts with the following proteins ILK1 CBL9- CIPK23 AKT1 CIPK23 acts to phosphorylate HAK5, the phosphorylation is what activates the HAK5 to take up K+. (wikipedia.org)
  • High Affinity K+ transporter HAK5, is important for the regulation of K+ ions within the cell. (wikipedia.org)
  • Uptake of [H-3]uracil at 22 degrees C was rapid and saturable and appeared to be mediated by a single high-affinity transporter, designated U1, with an apparent K-m of 0.46 +/- 0.09 mu M and a V-max of 0.65 +/- 0.08 pmol. (kent.ac.uk)
  • These data show that uracil uptake in T. b. brucei procyclics is mediated by a single high-affinity transporter with high substrate selectivity and are consistent with a nucleobase-H+-symporter model for this carrier. (kent.ac.uk)
  • Deletion of SLC19A2, the high affinity thiamine transporter, causes selective inner hair cell loss and an auditory neuropathy phenotype. (medlineplus.gov)
  • Here, we report a group of high-affinity non-Region I rabbit monoclonal antibodies. (nih.gov)
  • One pair of antibodies (YP218 and YP223) is suitable to detect soluble mesothelin in a sandwich ELISA with high sensitivity. (nih.gov)
  • In this study, murine-sourced scFv library were screened by phage display technology against human Scl-70, and the scFvs with high affinity were constructed into humanized antibodies for clinical application. (bvsalud.org)
  • Moreover, these humanized antibodies showed high specificity for Scl-70 in diagnostic immunoassays for ANA. (bvsalud.org)
  • Applications should describe an approach for improving and/or developing protein affinity reagent technologies that can be ready for proteome scale projects within three to five years. (nih.gov)
  • This FOA requests applications that will develop an affinity reagent resource of the broadest possible utility, including detection of the target protein in a sample, immuno-histochemical labeling of a tissue sample, or immunoprecipitation or other affinity capture of the target protein. (nih.gov)
  • This FOA requests proposals that will develop an affinity reagent resource for human transcription factors of the broadest possible utility, including possible detection of the target protein in a sample, immuno-chemical labeling of a tissue sample or cells, or immunoprecipitation of the target protein. (nih.gov)
  • In particular, we show how models initially induced from small data sets can extrapolatively identify potent new ligands with novel underlying scaffolds with very high specificity. (springer.com)
  • Human-murine chimeric autoantibodies with high affinity and specificity for systemic sclerosis. (bvsalud.org)
  • This orthopoxvirus MHC class I-like protein (OMCP) is conserved among cowpox and monkeypox viruses, secreted by infected cells, and bound with high affinity by NKG2D of rodents and humans (K(D) approximately 30 and 0.2 nM, respectively). (nih.gov)
  • Desensitization was seen after exposing cells to the agonist quinpirole for 1.5 hr, suggesting that the rapid loss of high affinity binding sites represents an uncoupling of the receptor from the G protein that mediates inhibition of adenylyl cyclase. (aspetjournals.org)
  • Pretreatment of cells with the protein synthesis inhibitor cycloheximide did not block the quinpirole-induced loss of receptors with a high affinity for agonists. (aspetjournals.org)
  • reproducibility and high protein yield. (nih.gov)
  • The protein Avidin has an extremely high affinity for the cofactor Biotin (Kdiss 10-15M). (sigmaaldrich.com)
  • For the FEP approach, affinity predictions are made by estimating the difference in the free energy of protein-ligand complexes between closely-related ligand pairs (typically just a few substituent atoms are different). (springer.com)
  • The physicochemical properties of the amino acid sequence , three-dimensional structural basis, and electrostatic potential distribution of the protein surface of different scFv fragments revealed differences in the electrostatic potential of their CDR regions determined their affinity for Scl-70 and expression. (bvsalud.org)
  • Prominent rare-coin and precious-metals dealer Michael Fuljenz of Beaumont, Texas, an NRA Golden Ring of Freedom member, is the 2023 recipient of the Chester L. Krause Distinguished Service Award-the highest honor bestowed by the congressionally chartered American Numismatic Ass'n, the largest organization of coin-collecting enthusiasts in the United States. (americanrifleman.org)
  • In the past few years, several laboratories have begun to use high-pressure liquid chromatography (HPLC) as a screen for hemoglobinopathies. (medscape.com)
  • Sephadex A-50 ion-exchange chromatography of haemolysate showed that different types of haemoglobin had different affinities for lead. (bmj.com)
  • Further analysis with carboxylmethyl (CM) cellulose chromatography indicated that the gamma globin of fetal origin had a higher affinity for 203Pb than the beta globin, whereas alpha globin appeared to be unimportant in lead binding. (bmj.com)
  • Affinity chromatography is the preferred method of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. (sigmaaldrich.com)
  • These resins may be used directly for adsorpotion of compounds having an affinity for the particular amino acid immobilized, or they can serve as functionalised linkers and allow covalent attachment through their free carboxyl terminus to a ligand containing a free primary amine. (sigmaaldrich.com)
  • QuanSA predictions yield binding affinities, explicit estimates of ligand strain, associated ligand pose families, and estimates of structural novelty and confidence. (springer.com)
  • H-3]Uracil uptake was not inhibited by a broad range of purine and pyrimidine nucleosides and nucleobases (concentrations up to 1 mM), with the exception of uridine, which acted as an apparent weak inhibitor (K-i value of 48 +/- 15 mu M). Similarly, most chemical analogues of uracil, such as 5-chlorouracil, 3-deazauracil, and 2-thiouracil, had little or no affinity for the U1 carrier. (kent.ac.uk)
  • Infigratinib, a selective FGFR1-3 tyrosine kinase inhibitor, alters dentoalveolar development at high doses. (nih.gov)
  • Because the migration of many high-oxygen-affinity variants on alkaline and acid gels does not differ from that of HbA, oxygen-hemoglobin dissociation studies are often used to document their presence. (medscape.com)
  • High-affinity CD16 variants in the human population correlate with better clinical outcome and anti-tumor response. (bmj.com)
  • Master vector illustration in Affinity Designer on the iPad. (udemy.com)
  • Exposure to agonists decreased the proportion of receptors in the high affinity, agonist-preferring state. (aspetjournals.org)
  • Exposure to antagonists, however, led to an increase in the density of receptors with a high affinity for agonists. (aspetjournals.org)
  • The effect of (-)-sulpiride on high affinity binding sites was blocked by cycloheximide, but only after incubation of cells for sufficient time to induce an increase in the total number of receptors. (aspetjournals.org)
  • After incubation of cells with (-)-sulpiride for a short time, the increase in the number of receptors with a high affinity for agonists was unaffected by cycloheximide. (aspetjournals.org)
  • Previous studies showed that whereas the potent 5-HT3 receptor antagonist only labels 5-HT3 receptor binding sites, the , labels these receptors and another class of high-affinity binding sites, named the R sites, in membranes from the rat cerebral cortex and NG 108-15 clonal cells (Kidd et al. (cardiff.ac.uk)
  • We now present a series of peptides, related to and including NPC 18325, that explore the relationship between the length of the carbon chain and the affinity to the human bradykinin B2 receptor. (nih.gov)
  • The results show that there is a structure-activity relationship (SAR) associated with the chain length and that these pseudopeptides have better affinity to the human bradykinin receptor than they have to the guinea pig ileal-derived B2 receptor. (nih.gov)
  • Agonists and antagonists differentially regulate the high affinity state of the D2L receptor in human embryonic kidney 293 cells. (aspetjournals.org)
  • These findings have been extended by using a radiolabeled agonist to investigate agonist- and antagonist-induced regulation of the high affinity state of the D2L dopamine receptor in transfected HEK 293 cells. (aspetjournals.org)
  • Bromine-76-radiolabeled analogues of previously reported high-affinity A(3) adenosine receptor (A(3)AR) nucleoside ligands have been prepared as potential radiotracers for positron emission tomography. (nih.gov)
  • Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. (nih.gov)
  • The high-affinity cellular receptor for the basement membrane component laminin is differentially expressed during tumor invasion and metastasis. (nih.gov)
  • The study of binding affinity is essential in surface plasmon resonance (SPR) sensing because it allows researchers to quantify the affinity between the analyte and immobilised ligands of an SPR sensor. (mdpi.com)
  • Because this FOA will support a production activity, it is anticipated that the center(s) will emphasize the advantages of high throughput and scalability to be achieved within a production effort, and will for example develop process improvements, thereby improving cost and quality over the duration of the award. (nih.gov)
  • Christian ST, CmClain LD, Morin RD, Benington F. "Blockage of LSD Binding at its High Affinity Site on Synaptosomal Membranes by l-Methyl-1,2,5,6-Tetrahydropyridine-N,N-Diethyl- carboxamide" Experientia . (erowid.org)
  • If the soil has a high salinity content, this means Na+ from NaCl competes with K+ for uptake because they are similar ions and use the same transporters. (wikipedia.org)
  • Our study found that mouse mB expressed TLR9 and 4, and responded to their agonists in vitro by differentiating into high affinity IgG secreting plasma cells. (umd.edu)
  • When immunized together with the antigen, TLR agonists not only boosted the antigen-specific titers, but also increased affinity and isotype switching of the immunoglobulin. (umd.edu)
  • The Franchi Affinity Turkey Elite provides a robust inertia-driven operating system wrapped within an aluminum receiver featuring Cerakote's Midnight Bronze finish. (americanrifleman.org)
  • This KB represents an affinity 2 orders of magnitude greater than that of the monometallic analogue [Pt(tpy)(pic)] 2+ for the same dsDNA sequence. (psu.edu)
  • The resultant products exhibited the expected high affinity (K(i) approximately 0.6 nM) and specific binding at the human A(3)AR in vitro. (nih.gov)
  • The high polymorphism of HLA gene is responsible for the complicated ability of the immune system to react to a tremendous variety of different peptides 4 , 5 . (nature.com)
  • We show the DTX3L D3 domain (230-510) mediates the interaction with PARP9 with nanomolar affinity and an apparent 1 : 1 stoichiometry. (portlandpress.com)
  • Unless conditions exist for reducing nitrate to nitrite in the gut (i.e., high pH and proper intestinal microbial flora), ingested nitrate (NO 3 -) is metabolized and excreted without producing apparent adverse effects. (cdc.gov)
  • Affinity Photo's tools and commands have a depth of functionality that will take some time to explore and master. (tomsguide.com)
  • Different activating chemistries will impart unique characteristics to the reactivity and functionality of the affinity matrix. (sigmaaldrich.com)
  • By turning on, it increases the plants affinity (uptake) of potassium. (wikipedia.org)
  • In particular, synthetic analogues of Δ 9 -THC with a longer side chain have shown cannabimimetic properties far higher than Δ 9 -THC itself. (nature.com)
  • New Art/Science Affinities" (2011, 8.5x11 inches, 190 pages, perfect-bound paperback, 232 full-color illustrations) is available for purchase ($45.75) through print-on-demand service Lulu, or for free download via the Miller Gallery website (http://www.cmu.edu/millergallery/nasabook). (cmu.edu)
  • In addition, you gain a +2 competence bonus on all Survival checks made at altitudes of 5,000 feet or higher. (d20pfsrd.com)
  • By the end of this course you will be able to turn your sketches into professional high quality vector illustrations that will blow away your friends and family. (udemy.com)
  • The principal mission of the Light Imaging Section is to give NIAMS scientists access to state-of-the-art equipment and to offer training and assistance in the acquisition and analysis of high quality scientific images by light microscopy. (nih.gov)
  • The Ag- specific B cells that had not yet undergone isotype switching showed a relatively higher expression of TLR4 than memory B cells, which was reflected in a heightened response to its agonist, but in both cases of TLR4 and 9 yielded mostly low affinity IgM secreting plasma cells. (umd.edu)
  • It has previously been thought that a single zinc finger of the Cys2-His2 family is incapable of specific, high-affinity binding to DNA. (nih.gov)
  • While affinity group members felt good about the work being done, the greater corporate community began to view these groups as a type of company-sanctioned social club. (diversityjournal.com)
  • These investigators had postulated, on the basis of structure activity comparisons, that THPC in sufficiently high concentrations should, in fact, cross the blood brain barrier and act as a competitive antagonist of the hallucinatory activity of LSD. (erowid.org)
  • Osmosis Ion Homeostasis Immune Response Growth Higher levels of potassium in the roots creates a greater amount of photosynthesis in the leaves by helping to control osmosis occurring throughout the cells. (wikipedia.org)
  • Furthermore, we have engineered a humanized YP218 Fv that retains full binding affinity for mesothelin-expressing cancer cells. (nih.gov)
  • Conclusions This work demonstrates a powerful new method to drive high-level constitutive hCD16 expression on the surface of iNK cells through transgene knock-in at the GAPDH locus using an engineered AsCas12a. (bmj.com)
  • The high level constitutive hCD16 expression enhances ADCC of iNK cells and enables enhanced serial tumor killing and is expected to exert enhanced anti-tumor activity in the clinic. (bmj.com)
  • Since we have been working for some time inour laboratory characterizing the properties of the high affinity for LSD binding site on synaptosomal memberanes from rat brain, we were intrigued by the idea of obtaining molecular (rather than behavioral) evidence for the blockade of LSD binding by THPC. (erowid.org)
  • We also show that DTX3L and PARP9 assemble into a higher molecular weight oligomer, and that this is mediated by the DTX3L N-terminal region (1-200). (portlandpress.com)
  • This affinity between images in history and images in the present points to the importance of teasing out what architects call the discipline of architecture. (archdaily.com)
  • For the haemolysate from adults, lead was present in both Hb A (alpha 2 beta 2) and Hb A2 (alpha 2 delta 2), whereas, in the haemolysate from new-born infants, the haemoglobin of fetal origin, Hb F (alpha 2 gamma 2) showed a much greater affinity for 203Pb than the adult haemoglobin Hb A (alpha 2 beta 2), obtained from maternal blood. (bmj.com)
  • Water pipes/hookahs, Furthermore, racial/ethnic and sociodemographic differences e-cigarettes, snus, and dissolvable tobacco products did not in the use of any tobacco product were observed, with higher have usage thresholds. (cdc.gov)
  • In addition, a collaboration between federal, state, and local law enforcement partners has resulted in the Stop Fraud Utah campaign, which aims to educate the public about affinity fraud-what people can do to avoid it and how best to report it if they have been victimized. (fbi.gov)
  • While Archive of Affinities is a deeply personal pursuit, it is also extremely public. (archdaily.com)
  • For instance, Serif is currently going through the extensive list of third-party Photoshop-compatible plugins to ensure they work properly in Affinity Photo. (tomsguide.com)
  • Smaller prints need to be scanned at a higher resolution to work effectively with them. (serif.com)
  • On the range, the Franchi Affinity 3 Turkey Elite proved its ability to consistently print patterns on target, and at distance. (americanrifleman.org)
  • In terms of higher-level relationships, "sparassocynids" have been consistently identified as "didelphimorphians" (Forasiepi et al. (springer.com)
  • Solution Architecture For SMP node affinity contains definitions that the reader may find provide helpful background. (whamcloud.com)