• Quantitative real-time polymerase chain reaction (PCR) is regarded as the gold standard for molecular profiling and target identification, but not in the context of high-throughput screening owing to limitations on workflow, cost of reagents, and miniaturization opportunities. (nih.gov)
  • We present, for the first time, data from a vendor-independent one-step 1536 workflow that compares reporter gene and RT-qPCR screening approaches for oncology drug discovery. (nih.gov)
  • We also demonstrate a miniaturized and high-throughput workflow that could enable future application of this sensitive assay technology, with particular impact against phenotypic assays and those using rare cell types. (nih.gov)
  • MRSA screening workflow. (nih.gov)
  • These displaying phages can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those other molecules. (wikipedia.org)
  • The assay utilizes a simple mix-and-read format with a far-red positive TR-FRET readout, ensuring its compatibility with automated dispensing equipment and plate readers commonly used for HTS. (selectscience.net)
  • Many countries using sensitive screening methods for detection of carriage of methicillin-resistant Staphylococcus aureus (MRSA) have a sustained low incidence of MRSA infections. (nih.gov)
  • The assays described take advantage of the ability of lipid hydroperoxides to oxidize nonfluorescent diphenyl-1-pyrenylphosphine (DPPP) to a fluorescent phosphine oxide. (diva-portal.org)
  • The TH disrupting potency of 22 herring gull (Larus argentatus) egg extracts, collected from two different locations (Musvær and Reiaren) in Norway, was evaluated to demonstrate the applicability of the assay for environmental samples. (bibsys.no)
  • These one-step reagents enable the user to go straight from a cellular assay format to qPCR without the need for cumbersome and potentially expensive multistep RNA purification protocols. (nih.gov)
  • Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to inclu. (nih.gov)
  • Herein we designed oligonucleotides for a new nuc targeted hydrolysis probe PCR to replace the standard in-house nuc SybrGreen PCR assay. (nih.gov)
  • We have screened the NIH collection of known drugs-a library of 727 drugs that have been approved for the treatment of various indications. (biomedcentral.com)
  • We used the M30-ELISAassay to screen 500 low molecular weightcompounds from a chemical library from theNational Cancer Institute and identified 16drugs with strong pro-apoptotic activity,suggesting that the assay is a useful toolfor discovery of pro-apoptotic drugs. (springer.com)
  • Currently, the most successful bio-analytical method to detect and evaluate TH disruptors, which target the plasma transport of TH in environmental samples, is the radio-ligand thyroxine-transthyretin (T4-TTR) binding assay. (bibsys.no)
  • Tox21 currently pools the resources and expertise of EPA, National Institutes of Environmental Health Sciences/National Toxicology Program, National Institute of Health/National Center for Advancing Translational Sciences, and the Food and Drug Administration to screen almost 10,000 chemicals. (topcoder.com)
  • Yet, costly materials and tedious handling procedures prevent the use of this assay in high throughput analysis that is nowadays urgently demanded in environmental quality assessment. (bibsys.no)
  • The screening data will be used to more cost-effectively and efficiently prioritize the thousands of chemicals that need in vivo toxicity testing and, in the future, predict the potential toxicity of chemicals. (topcoder.com)
  • In the present preliminary screening, we evaluated two hundred chemicals chosen at random. (go.jp)
  • We show that this assay detectsapoptosis in epithelial cells and that thesensitivity is sufficient for screening inthe 96-well format. (springer.com)
  • A high-capacity analysis machine can measure dozens of plates in the space of a few minutes like this, generating thousands of experimental datapoints very quickly. (wikipedia.org)
  • Typically, an integrated robot system consisting of one or more robots transports assay-microplates from station to station for sample and reagent addition, mixing, incubation, and finally readout or detection. (wikipedia.org)
  • For diagnostic laboratories with high sample volumes, MRSA screening requires stability, low maintenance and high performance at a low cost. (nih.gov)
  • Two High Throughput Screen Assays for Measurement of TNF-α in THP-1 Cells. (sigmaaldrich.com)
  • The assay was validated in a whole-genome siRNA library screening setting using PC-3 prostate cancer cells. (biomedcentral.com)
  • In a high-throughput screening setting, using 4 Gy irradiated and control PC-3 cells, the effects of DNA-PKcs siRNA and non-targeting control siRNA could be clearly discriminated. (biomedcentral.com)
  • Using this assay, we demonstrated that 12G5 specifically binds to the CXCR4-expressing cells, but not to CCR5-expressing cells and cells without CXCR4 and CCR5, consistent with the results obtained by using flow cytometry. (neb.com)
  • With the increasing availability of laboratory automation, some studies are starting to explore the possibility of conducting limited, semi-automated versions of the assay. (eurekaselect.com)
  • By drugging our download High and Combining to our Comments Study, you offer to our history of results in range with the readers of this company. (lustige-weihnachtsgeschenke.de)
  • A screening facility typically holds a library of stock plates, whose contents are carefully catalogued, and each of which may have been created by the lab or obtained from a commercial source. (wikipedia.org)