High specificity to mitochondria. Medical search. Frequent questions

Unfortunately, the available biomarkers for SKM injury do not fully meet the needs for satisfactory detection of drug-induced damage, both in clinical and research settings, mainly due to their low sensitivity and specificity. (degruyter.com)
This approach aids pharmaceutical research in solving drug discovery problems with high specificity and sensitivity with high temporal and spatial resolutions. (visikol.com)
To test the specificity and sensitivity of the PELE-Seq method, we sequenced control E. coli DNA libraries containing known rare alleles present at frequencies ranging from 0.2-0.4% of the total reads. (umn.edu)
PELE-Seq had higher specificity and sensitivity than standard libraries. (umn.edu)
The instruments are tuned to collect data on only the analytes of interest, optimizing sensitivity, specificity and quantitative accuracy. (cedars-sinai.edu)
It has high sensitivity and specificity in diagnosing HCC and is negatively associated with prognosis. (biomedcentral.com)
A comparison of calls for the HapMap samples to existing genotype data suggested 92% sensitivity and 99.6% specificity, at sites where coverage was 100x or greater. (massgenomics.org)

Although studies suggest that αSyn and its missense mutant, A53T, preferentially accumulate in the mitochondria, the mechanisms by which αSyn and mitochondrial proteins regulate each other to trigger mitochondrial and neuronal toxicity are poorly understood. (springer.com)
Both proteins can localize at the mitochondrion , but the way they control apoptosis still remains unclear. (sdbonline.org)
biochemical abnormal proteins( PUFA) rectifying new synthase specific TREK mitochondria. (familie-vos.de)
In plants, PPR proteins localize almost exclusively to mitochondria and chloroplasts, with functions including RNA, stabilization, splicing and editing. (plantae.org)
used insights about the amino-acid to RNA code to design artificial PPR (aPPRs) proteins that bind target RNAs with high specificity in vivo. (plantae.org)
The details of how proteins are moved around within a cell are being deciphered with high-powered microscopy combined with sophisticated techniques for labeling individual molecules, including fluorescent or "quantum dot" tags and antibodies tailored to adhere to a protein in a particular state of activation. (alzforum.org)
Proteinase K finds application in in-vitro diagnostics, in the treatment of paraffin-embedded tissue sections during antibody labelling, extraction of mitochondria from hepatic cells, digestion of proteins from clinical tissue samples, and the analysis of the localization of enzymes on membranes. (pharmiweb.com)
I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
The two inactive halves, 'Split-DddA tox halves' were also separately tethered to TALE proteins and tagged with a mitochondria targeting amino acid sequence. (wats-on.co.uk)

The results of three enzyme activities reflect significant both tissue- and population specificity. (researchsquare.com)
Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. (nature.com)
Comparing phospholipid profiles of mitochondria and whole tissue: Higher PUFA content in mitochondria is driven by increased phosphatidylcholine unsaturation. (oregonstate.edu)
Finally, the nasal lesions associated with exposure to high concentrations of MeI in rats are best supported by a mode of action that involves glutathione (GSH) depletion in the nasal epithelial tissue. (cdc.gov)

Positively charged CTAC could actively target mitochondria by interacting with the negatively charged mitochondria membrane, and then lead to the dysfunction of mitochondria by decreasing mitochondrial potential and intracellular ATP levels, resulting in the necrosis and apoptosis of MCF-7 cells. (rsc.org)
Mitochondrion is a common membrane-coated organelle that participates in energy conversion, homeostasis of calcium, cell signaling and cell death. (rsc.org)
4 The mitochondrial membrane potential of cancer cells (∼−220 mV) is more negative than that of normal cells (∼−160 mV). 5 Therefore, mitochondria have been regarded as a novel target for cancer therapy. (rsc.org)
9 Although the design of nanoparticles with triphenylphosphonium cation (TPP, the mitochondrion-targeting moiety) offers potential for mitochondrion-targeting therapeutics, it is still a challenge to actively deliver therapeutic agents to mitochondria due to the selective permeability of the mitochondrial membrane. (rsc.org)
At high concentrations piericidin also inhibited succinate oxidation, possibly due to damage of the mitochondrial membrane system. (warwick.ac.uk)

The proton leak of liver mitochondria in the Maduo population account for a lower percentage of state III than that of the Golmud population (11.4~14.6% VS. 22.5~25.1%), which indicate a higher ATP production in liver mitochondria. (researchsquare.com)
Kennedy, E. P. & Lehninger, A. L. Oxidation of fatty acids and tricarboxylic acid cycle intermediates by isolated rat liver mitochondria. (nature.com)
Lardy, H. A. & Adler, J. Synthesis of succinate from propionate and bicarbonate by soluble enzymes from liver mitochondria. (nature.com)
Alcoholic ketoacidosis: Ketone body production is stimulated by altered redox status within the liver mitochondria. (medscape.com)

The aggregation of SNVs in the mitochondria respiratory chain suggests the pivotal role of those SNVs in the pathogenesis of RA with severe erosive arthritis. (nature.com)
These physical changes in mitochondria are brought about rapidly and reversibly by normal reactants of the respiratory chain. (rupress.org)
Their speed, specificity, and reversibility indicate that they are closely geared to the normal function of the respiratory chain and are a true reflection of a mechanochemical coupling process characteristic of the physiology of mitochondria. (rupress.org)
It was shown that piericidin was acting at the same site as rotenone in the NADH dehydrogenase region of the respiratory chain of beef heart mitochondria. (warwick.ac.uk)
This situation may occur at high altitude or during respiratory diseases, including chronic obstructive pulmonary disease, sleep apnoea and fibrosis, and during failure of ventilation due to neurological diseases. (ersjournals.com)

Exploring thermoregulation and metabolic characteristics responses to high altitudes is important for understanding ectothermic animal high-altitude adaptation mechanisms. (researchsquare.com)
ROS may originate from mitochondria and/or NADPH oxidases but the exact oxygen sensing mechanisms, as well as the question of whether increased or decreased ROS cause HPV, are under debate. (ersjournals.com)
This is particularly useful for mtDNA genome editing since the mechanisms to repair such DNA damage are inefficient in mitochondria. (wats-on.co.uk)

Bioactivable nanocarrier systems have favorable characteristics such as high cellular uptake, target specificity, and an efficient intracellular release mechanism. (acs.org)
Mitochondria play an important role in supplying cellular energy, cell signaling and governing cell death. (rsc.org)
Mitochondrial porin , also known as the voltage-dependent anion channel (VDAC), is a multi-functional channel protein that shuttles metabolites between the mitochondria and the cytosol and implicated in cellular life and death decisions. (sdbonline.org)
Mitochondria are multifaceted organelles with key roles in anabolic and catabolic metabolism, bioenergetics, cellular signalling and nutrient sensing, and programmed cell death processes. (nature.com)
However, recent advances in high-content screening have made it possible to get a better understanding of how drugs impact cellular pathways, accelerating the drug discovery process. (visikol.com)
High-content screening is a type of drug discovery method that utilizes high-throughput imaging and analysis to assess multiple endpoints simultaneously at a cellular resolution. (visikol.com)
This protein is found in a cellular compartment called the mitochondria, which is responsible for supplying energy to the rest of the cell and therefore plays an important role in metabolic processes. (elifesciences.org)
2] Oncocytic cells in the thyroid are often called Hürthle cells, and oncocytic change is defined as cellular enlargement characterized by an abundant eosinophilic granular cytoplasm as a result of accumulation of altered mitochondria. (medscape.com)

The protein is found in the nucleus and could also be detected in the cytoplasm or the mitochondria. (wikipedia.org)

IgG autoantibody testing of a subsample of stored serum from 1999-2004 participants was conducted to estimate the prevalence and specificities of selected autoantibodies in the US population. (cdc.gov)

Two types of swelling-shrinkage change manifested by isolated mammalian heart mitochondria have been studied. (rupress.org)

The DOX-loaded mPEG-(ss-TPP) 2 NPs were characterized in terms of their physicochemical and morphological properties, drug-loading and release behaviors, in vitro anticancer effects, and mitochondria-targeting capacity. (acs.org)
To this end, we report cetyltrimethylammonium chloride-loaded mesoporous silica nanoparticles conjugated with human serum albumin (CTAC@MSNs-HSA) as a mitochondrion-targeting agent for anticancer treatment. (rsc.org)

The resulting images showed the effects of the drugs on the ER network, cytoskeleton, and mitochondria. (visikol.com)

PELE-Seq is also well-suited for whole genome sequencing of mitochondria and viruses, and for high-throughput rare mutation screens. (umn.edu)
Mitochondria, the cell's energy producing organelles, have a significant role in biological functions as well a key role in disease, so a method to precisely edit the mtDNA genome has been a long sought-after goal. (wats-on.co.uk)

some biochemical properties of mitochondria and submicroscopic particulate material. (nature.com)

To study the structural basis of pathological remodelling and altered calcium channel functional states in the heart, we sought to re-purpose high-affinity ligands of the cardiac calcium channel, the ryanodine receptor (RyR2), into super-resolution imaging probes. (whiterose.ac.uk)
High‐throughput profiling of the mitochondrial proteome using affinity fractionation and automation. (nature.com)
CAGCCGGGACAACAGTTCAGGAATG-3¢) the authors then introduced the first tetracysteine sequence coding for FLNCCPGCCMEP*, a tetracysteine sequence with optimized flanking amino acids for particularly high affinity for biarsenical ligands (Martin et al. (typeset.io)
The SUR1 isoform encodes the high-affinity sulfonylurea receptor, which is found in pancreatic beta-cells, while the SUR2 isoform encodes the low-affinity sulfonylurea receptor, which is mainly present in mitochondria-rich cardiac, smooth and skeletal muscles. (warf.org)
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. (thermofisher.com)

In our previous study, it was found that GLS1 was highly expressed in tumour tissues, and the expression gradually increased from low-grade dysplastic nodules, to high-grade dysplastic nodules, to early- and advanced-stage HCC [ 14 ]. (biomedcentral.com)

The possible nature, concentration and specificity of this site was examined. (warwick.ac.uk)
This showed hEPVH to be distributed throughout HEK 293 cells but in highest concentration within mitochondria. (figshare.com)

The few dozens of maxicircles carry information equivalent to that of the mtDNA from higher eukaryotes while the several thousand varied minicircles carry info for RNA editing in the form of guidebook RNA (gRNAs) that direct extensive modification of the maxicircle mRNA transcripts [1]. (healthandwellnesssource.org)
Editing technologies based on CRISPR have had little success with mtDNA due to the inability to transport guide RNA, a major component of CRISPR-Cas9 technology, into the mitochondria. (wats-on.co.uk)
Furthermore, TALEN technology cannot be utilised in individuals with a high mutation load as there is a risk that mtDNA copy numbers would reduce to detrimental levels in these individuals. (wats-on.co.uk)
have demonstrated that targeted base edits in mtDNA can be made in human cells with high specificity and low levels of off-target effects. (wats-on.co.uk)

Identification and Origination of the O*-Dominated β-NiOOH Intermediates with High Intrinsic Activity for Electrocatalytic Alcohol Oxidation. (acs.org)
Further studies of specificity of rotenone induced CL oxidation in rat midbrain and correlations with the unique stereo-specific molecular species of CL formed in mitochondria of human and rat lymphocytes in response to rotenone exposure will determine the extent to which these profiles of lymphocyte CL oxidation may lead to reliable biomarkers of mitochondrial dysfunction associated with neurodegenerative disorders. (cdc.gov)

Mitochondrial accumulation of FlAsH/Lumio Green was reversed by addition of low concentrations of thiol-containing reagents during labeling and a subsequent high stringency thiol wash. (typeset.io)

PKmito DEEP RED labels mitochondria in live cells with very high specificity. (cytoskeleton.com)
1 It has become increasingly evident that mitochondria play a key role in the generation, proliferation and metastasis of cancer cells. (rsc.org)
2 Mitochondria of cancer cells are different from those of normal cells in many ways. (rsc.org)
High-content screening differs from traditional drug discovery methods in that it provides richer datasets than traditional assays, allowing for the examination of the specific effect of compound treatments on specific sub-populations of cells, as well as providing access to more complex measurements than can be accomplished with traditional assay formats. (visikol.com)
Visikol's future perspectives include incorporating high-throughput robotics and automation to seed cells and deliver compounds to microplates, improving high throughput technology in imaging and its computational image processing pipeline by integrating the data generated with AI and machine learning tools. (visikol.com)
It has a 24-well capacity per plate for examining cells or isolated mitochondria. (cedars-sinai.edu)
found that when this variant form of ATP5G1 was introduced into the cells of mice, their mitochondria was better at coping with stress conditions, such as low oxygen, low temperature and poisoning. (elifesciences.org)

Using a gene editing tool to selectively substitute some of the building blocks, also known as amino acids, that make up the ATP5G1 protein revealed that improvements to the mitochondria were caused by switching specific amino acids. (elifesciences.org)
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. (ayassbioscience.com)
The use of proteinase K is particularly high among gene therapy for hereditary diseases including diabetes, down syndrome and many others. (pharmiweb.com)

The contents of CL molecular species with polyunsaturated fatty acid residues - C18:2, C20:4 and C22:6 - were as high as 67.7%, 24.7% and 5.4%, respectively. (cdc.gov)
Its easy-to-use touch screen interface and customizable workflow settings allows for rapid high-throughput acquisition of anatomical, hemodynamic, functional and molecular information. (cedars-sinai.edu)
Primarily, this new molecular tool does not require 'traditional' CRISPR RNA guides, exploiting existing transport machinery to transfer protein into mitochondria. (wats-on.co.uk)

This longer isoform may help regulate energy metabolism in the mitochondria. (ayassbioscience.com)

RUNX3 reports with heterodimer organelles previously are PIP3 and direct functional function in the potentiation of specificity acts( Huang et al. (familie-vos.de)

Score>80(red) has high confidence and is suggested to be used for WB detection. (affbiotech.com)

That may be due to the fact that while each cell contains exactly 2 copies of each nuclear chromosome, it contains numerous mitochondria and thus numerous copies of the MT chromosome (possibly 20-25 per cell, by one estimate). (massgenomics.org)

The development of heavy-atom-free photosensitizers (PSs) for photodynamic therapy (PDT) has encountered significant challenges in achieving simultaneous high fluorescence emission and reactive oxygen species (ROS) generation. (bvsalud.org)

Background Tc38 of Trypanosoma cruzi has been isolated as a single stranded DNA binding protein with high specificity for the poly [dT-dG] sequence. (healthandwellnesssource.org)

Finally, we examine the utility of an expanded, functionally annotated mitochondrial proteome in a translational setting for aiding both diagnosis of mitochondrial disease and targeting of mitochondria for treatment. (nature.com)

A set of CDC autoimmune reference sera (12 different specificities) (Chan EKL et al. (cdc.gov)

Widely expressed with high levels in heart, brain and placenta. (affbiotech.com)

In the present study, the selected body temperature, mitochondrial consumption, proton leak and enzyme activity of a lizard Phrynocephalus vlangalii from different altitudes (Maduo and Golmud, with altitude at 4270 and 2750 m, respectively) were analyzed to investigate the thermoregulatory and metabolic regulation strategies of this native high-altitude lizard at Qinghai-Tibet Plateau. (researchsquare.com)
These distinct variable characteristics between two populations of P. vlangalii could be considered important strategies in thermoregulation and metabolic regulation for living at different altitudes and could be especially necessary for lizards to effectively compensate for the negative influence of cold and hypoxia at high altitude. (researchsquare.com)

Cell Painting is a high-content, multiplexed image-based assay used for cytological profiling. (visikol.com)
High-content screening is a powerful tool for drug discovery, and Visikol's Cell Painting Assay is a game-changer in this field. (visikol.com)

Poor specificity (as an RyR2 imaging probe) was confirmed at higher resolution with expansion microscopy (proExM) (~70 nm). (whiterose.ac.uk)
It is one type of super resolution microscopy which allows the capture of images with a higher resolution than conventional light microscopy which is constrained by diffraction of light. (cytoskeleton.com)

Compositional analysis of ZNF548 revealed that Alanine was found at a lower percentage than expected while Histidine was found at a much higher percentage than expected in the human ZNF548. (wikipedia.org)
Two‐dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: toward a human mitochondrial proteome. (nature.com)

high GSH/GSSG ratio. (who.int)

Hürthle cell cancer has the highest incidence of metastasis among the differentiated thyroid cancers. (medscape.com)

These results demonstrate the potential of CTAC@MSNs-HSA in cancer therapeutics as well as providing insights into mitochondrion-targeting treatment. (rsc.org)
Feature papers represent the most advanced research with significant potential for high impact in the field. (mdpi.com)
Moreover, low production cost, increasing healthcare spending, and vast application are inducing high potential growth opportunities for the global proteinase K market participants. (pharmiweb.com)

6 Numerous research studies have been carried out to construct mitochondrion-targeted therapeutics. (rsc.org)
Their terminations contain numerous small vesicles and mitochondria. (cdc.gov)

In addition, factors like good product stability and specificity will underpin the target market growth. (pharmiweb.com)

Additionally, available antibodies yield variable results and have not been rigorously tested for specificity. (warf.org)

A second type of swelling-shrinkage mechanism, designated as phase II or "high amplitude," leads to changes in light scattering, viscosity, and mitochondrial volume which, frequently but not always, are of higher magnitude than the phase I type. (rupress.org)

In both cell types, the biarsenical staining reagent FlAsH/Lumio Green accumulated in active mitochondria and led to mitochondrial swelling. (typeset.io)
its subcellular localization in the initial parasite mitochondrion adjustments based on the cell routine stage. (healthandwellnesssource.org)
Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)

We do this by developing innovative software and high quality services for the global research community. (researchsquare.com)

Combinations of susceptibility genes are associated with higher risk for multiple sclerosis and imply disease course specificity. (cdc.gov)

Despite these findings suggesting that mitochondria might be a key link between αSyn toxicity and neuronal degeneration in PD, the field still lacks an understanding of how αSyn abnormality and mitochondrial functional deficiency influence each other. (springer.com)

Probably as a consequence of its evolutionary range from higher eukaryotes this protozoan shows several unique features. (healthandwellnesssource.org)

Anatomy22

Organisms13

Diseases1

Chemicals and Drugs108

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes63

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science5

Health Care7

Sensitivity7

Proteins9

Tissue4

Membrane5

Liver4

Respiratory5

Mechanisms3

Cellular8

Cytoplasm1

Autoantibodies1

Mammalian1

Anticancer2

Cytoskeleton1

Genome2

Biochemical1

Affinity5

Tissues1

Concentration2

MtDNA4

Oxidation2

Accumulation1

Cells7

Gene3

Molecular3

Regulate1

Organelles1

Detection1

Chromosome1

Fluorescence1

Poly1

Diagnosis1

Sera1

Widely1

Altitude2

Assay2

Microscopy2

Human2

Ratio1

Cancers1

Potential3

Numerous2

Target1

Results1

Mechanism1

Cell3

Research1

Multiple1

Functional1

Unique1