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Chromatography, High Pressure LiquidMass SpectrometryChromatography, LiquidGas Chromatography-Mass SpectrometrySpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryBiotransformationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationChromatography, Reverse-PhaseMolecular Sequence DataAmino Acid SequenceTerphenyl CompoundsMetabolomicsReproducibility of ResultsMolecular StructureProteomicsMagnetic Resonance SpectroscopyMetabolomeMicrosomes, LiverChromatography, Thin LayerFungicides, IndustrialBileIsotope LabelingPeptide FragmentsChromatography, GasGlucuronidesPeptidesSensitivity and SpecificityStereoisomerismCytochrome P-450 Enzyme SystemProteomeSpectrophotometry, UltravioletCattleChromatography, Ion ExchangeOxidation-ReductionCalibrationChromatography, GelLiverKineticsRats, Sprague-DawleyPeptide MappingChromatography, AffinityCarbohydrate SequenceTime FactorsReference StandardsMixed Function OxygenasesArea Under CurveMolecular WeightElectrophoresis, Polyacrylamide GelGlutathioneAryl Hydrocarbon HydroxylasesTrypsinCytochrome P-450 CYP3AAmino AcidsSpectrometry, Mass, Fast Atom BombardmentOligosaccharidesPlant ExtractsOpiate AlkaloidsRecombinant ProteinsDose-Response Relationship, DrugCarbohydrate ConformationVolatile Organic CompoundsLimit of DetectionSolid Phase MicroextractionChromatographyFatty AcidsIndicators and ReagentsHydroxylationHydrolysisMethanolDrug StabilitySoftwareChemistryHydroxyeicosatetraenoic AcidsBrain ChemistryChemical PhenomenaCyanogen BromideMethylationDisaccharidesBiological MarkersSubstrate SpecificityIsomerismQuality ControlHydrogen-Ion ConcentrationEucommiaceaeCarbon RadioisotopesPolysaccharidesLithocholic AcidPhenolsArachidonic AcidsSubstance Abuse DetectionLipoxygenaseDeuteriumSwineModels, ChemicalElectrophoresis, Gel, Two-DimensionalSolid Phase ExtractionEstersIonic LiquidsEscherichia coli
Tandem Mass SpectroDetectionUHPLCSupercritical fluid chromatoMetabolitesCharacterizationSeparation1995Electrospray ionizationSelectiveProteinsChemical compoundsDeterminationSpectrometerSpectrometricSerumQuantificationElemental compositionDetectorIndicationsAnalyticalMatricesIonizationMethodDetectIonicIdentificationChromatographicAnalysisInstrumentationCompositionAccurateLaboratorySpectraAnalyteConcentrationsStudyMethodsJournalSpectromUltra-highSampleResearchCompoundsValidationContentProductsLevelsAntioxidantActivity
Tandem Mass Spectro15
  • Title : Two-dimensional high performance liquid chromatography separation and tandem mass spectrometry detection of atrazine and its metabolic and hydrolysis products in urine☆ Personal Author(s) : Kuklenyik, Zsuzsanna;Panuwet, Parinya;Jayatilaka, Nayana K.;Pirkle, James L.;Calafat, Antonia M. (cdc.gov)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based measurements for PRA and PAC have become popular. (degruyter.com)
  • Terminal Residue and Dietary Risk Assessment of Atrazine and Isoxaflutole in Corn Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry. (bvsalud.org)
  • High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed to determine neurotransmitters levels due to its distinct advantages. (knaw.nl)
  • In the present work an isocratic enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the separation and quantitative determination of dextro - and levo -methorphan and their pharmacologically relevant metabolites, dextrorphan and levorphanol, respectively, in human blood samples. (univpm.it)
  • Hong, SS, Park, JH & Kwon, SW 2007, ' Determination of proteins in infant formula by high-performance liquid chromatography-electrospray tandem mass spectrometry ', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , vol. 845, no. 1, pp. 69-73. (elsevierpure.com)
  • The method uses on-line solid phase extraction coupled to high performance liquid chromatography and tandem mass spectrometry. (cdc.gov)
  • Liquid-liquid extraction was followed by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. (ojp.gov)
  • Qualitative and quantitative analysis of 4-MEC was performed by validated high performance liquid chromatography-tandem mass spectrometry methods. (erowid.org)
  • Fast atom bombardment tandem mass spectrometry of carotenoids. (oregonstate.edu)
  • Fast atom bombardment tandem mass spectrometry of the anti-parasitic agent pentamidine and its oxygenated metabolites. (oregonstate.edu)
  • Spanos GA, Schwartz SJ, van Breemen RB, Huang CH. High-performance liquid chromatography with light-scattering detection and desorption chemical-ionization tandem mass spectrometry of milk fat triacylglycerols . (oregonstate.edu)
  • Concentrations of fenbendazole and praziquantel were established through high performance liquid chromatography-tandem mass spectrometry. (nel.edu)
  • Screening used a combination of tandem mass spectrometry, molecular technologies and biochemical analysis. (who.int)
  • The most optimal cutoff values for serum cortisol as measured by immunoassay and for salivary cortisol and salivary cortisone as measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were 376, 8.6, and 33.5 nmol/L, respectively, for post-LDSST peak values, and 170, 1.7, and 12.5 nmol/L, respectively, for basal values. (medscape.com)
Detection15
  • A High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) Qualitative Detection Method Developed for In Vivo Analyses of Toxin Orellanine from the Cortinarius orellanus Fr. (hindawi.com)
  • The high-performance liquid chromatography-mass spectrometry (HPLC-MS) qualitative analysis method in negative mode of detection was used to record the presence of orellanine in the stomach contents of rats after ingestion of Cortinarius orellanus . (hindawi.com)
  • Conifer seed oils from European Larch (Larix decidua), Norway Spruce (Picea abies) and European Silver Fir (Abies alba) have been analyzed by non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with atmospheric pressure chemical ionisation (APCI)-MS detection. (unboundmedicine.com)
  • Liquid chromatography coupled with amperometric detection proved to be insufficiently selective to quantify gluconate in radioactive effluents due to the complexity of the matrix. (hal.science)
  • The use of mass spectrometry with high performance liquid chromatography is based on the main ideas of those two techniques, the principles of physical detection of compounds within mixtures applied by liquid chromatography and the capabilities of mass analysis mass spectrometry is aimed to. (studykraken.com)
  • The procedure of the use of mass spectrometry with high-performance liquid chromatography can be explained as follows, the compounds should come through the liquid chromatography process of physical detection and then, the chemical components are measured with mass-to-charge ratio, the instrument of mass spectrometry (Potter 1995). (studykraken.com)
  • Sensitive, selective and reliable high-performance liquid chromatography (HPLC)-diode array detection (DAD)/electrospray ionization multi-stage mass spectrometry (ESI-MS n ) methods have been developed for the characterization of nine 2-glucosyloxycinnamic acid derivatives and quantitative analysis of three of the major 2-glucosyloxycinnamic acids, cis -melilotoside, trans -melilotoside and dihydromelilotoside, present in Dendrobium medicinal plants. (edu.hk)
  • Detection is achieved in the mass spectrometry (MS) via ionization of the analytes in the chromatographic effluent. (pall.com)
  • Often times, oxidized lipid species can co-chromatograph with non-oxidized species making the detection of the former extremely difficult, even with the use of mass spectrometry. (cdc.gov)
  • In this study, a normal-phase and reverse-phase two dimensional high performance liquid chromatography (HPLC)-mass spectrometric system was applied to separate oxidized phospholipids from their non-oxidized counterparts, allowing unambiguous detection in a total lipid extract. (cdc.gov)
  • The first step may also involve a separation technique, such as chromatography , and the second a detection / measuring device. (newworldencyclopedia.org)
  • If the concentration of element or compound in a sample is too high for the detection range of the technique, it can simply be diluted in a pure solvent. (newworldencyclopedia.org)
  • Mass spectroscopy is one of a number of innovative technologies that are being or have been developed for detection and identification of biological warfare and bioterrorism agents. (msdmanuals.com)
  • For a deeper insight into mass spectrometric detection the LifeScience course "Mass spectrometry for small molecules" is recommended.course covers modern liquid chromatography (HPLC) especially for bioanalytical applications. (lu.se)
  • Instrumentation and simple troubleshooting for high-performance liquid chromatography with universal and selective detection. (lu.se)
UHPLC4
  • A 'suspect screening analysis' method for grape metabolomics by ultra-high performance-liquid chromatography (UHPLC) and highresolution quadrupole-time of flight (QTOF) mass spectrometry was recently developed. (unicatt.it)
  • SwRI's instrumentation for GLP 5-batch analysis includes a triple quadrupole (QQQ) mass spectrometer (TQMS) with an ultra-high performance liquid chromatography (UHPLC) separation module. (swri.org)
  • The samples were analyzed using ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). (usda.gov)
  • This course covers modern liquid chromatography (HPLC and UHPLC) that are especially used for bioanalytical applications. (lu.se)
Supercritical fluid chromato2
  • J. Prothmann, S. Palmer, C. Turner, M. Sandahl, Separation of monomeric and dimeric phenolic compounds in lignosulphonate lignin on different stationary phases using ultrahigh-performance supercritical fluid chromatography, J. Chromatogr. (lu.se)
  • Bioanalytical HPLC includes advanced theoretical treatment of liquid chromatographic separation modes and the underlying distribution and adsorption equilibria including reversed-phase chromatography, normal-phase chromatography, ion chromatography, ion-pair chromatography, hydrophilic interaction liquid chromatography (HILIC), size exclusion chromatography and supercritical fluid chromatography. (lu.se)
Metabolites1
  • The study also found that the proportion of these urinary metabolites varied considerably depending on whether the subjects had high or low acute exposures or whether the subjects had general environmental exposures (i.e., not agricultural workers). (who.int)
Characterization1
Separation1
  • Li K, Al-Rudainy B, Sun M, Wallberg O, Hulteberg C, and Tunå P. Membrane Separation of the Base-Catalyzed Depolymerization of Black Liquor Retentate for Low-Molecular-Mass Compound Production. (lu.se)
19951
  • L'article présente les données collectées, depuis le commencement du programme en janvier 1995 jusqu'en décembre 2011, sur l'incidence des troubles dépistés ainsi que la base moléculaire des cas positifs dépistés. (who.int)
Electrospray ionization1
  • About 75% of LC-MS users utilize Electrospray Ionization (ESI) as the interface between liquid chromatography and MS. However, despite the versatility of ESI, this ionization technique suffers from a phenomenon known as the matrix effect. (pall.com)
Selective1
  • Thus, like other lipophilic drugs, fluoxetine has a wide volume of distribution, between 12 and 97 L/Kg, which indicates high accumulation in tissues ( Hiemke, Hartter, 2000 Hiemke C, Hartter S. Pharmacokinetics of selective serotonin reuptake inhibitors. (scielo.br)
Proteins3
  • Mass spectrometry can detect various proteins of different masses in a specimen. (msdmanuals.com)
  • Specific pathogens have unique proteins, and the relative mass and abundance of each protein can sometimes be used to identify a microorganism. (msdmanuals.com)
  • The levels of the binding proteins, cortisol-binding globulin (CBG) and albumin, may also contribute to the variation in cutoff levels and the performance characteristics of the post-SST serum total cortisol. (medscape.com)
Chemical compounds1
Determination3
  • Asati A, Satyanarayana GNV, Panchal S, Thakur RS, Ansari NG, Patel DK (2017) Ionic liquid based vortex assisted liquid-liquid microextraction combined with liquid chromatography mass spectrometry for the determination of bisphenols in thermal papers with the aid of response surface methodology. (springer.com)
  • Castro G, Rodríguez I, Ramil M, Cela R (2019) Direct analysis in real time accurate mass spectrometry determination of bisphenol A in thermal printing paper. (springer.com)
  • Djordjevic S, Kovacevic I, Miljkovic B, Vuksanovic J, Pokrajak M. Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: application to clinical study. (scielo.br)
Spectrometer3
  • The mass spectrometer was operated in scheduled multiple reaction monitoring (MRM) mode, with four transitions for each dextromethorpan, levomethorphan, dextrorphan and dextromethorphan-d3 and two transitions for each levorphanol, levorphanol-d3 and dextrorphan-d3. (univpm.it)
  • Parameters such as paper size, and paper position relative to the mass spectrometer inlet were evaluated. (springer.com)
  • The analyses were performed in selected ion monitoring mode on a linear ion trap mass spectrometer. (springer.com)
Spectrometric3
  • The ability of the two dimensional HPLC-mass spectrometric system to detect and characterize oxidized lipid products will allow new studies to be formulated to probe the answers to biologically important questions with regard to oxidative lipidomics and cellular insult. (cdc.gov)
  • Matrix selection in the FAB mass spectrometric analysis of synthetic dyes. (oregonstate.edu)
  • For accurate quantitative or qualitative analysis, liquid chromatography is today combined with different mass spectrometric techniques. (lu.se)
Serum3
  • 3 mmol/L), lower median numbers of leukocytes in cerebrospinal fluid, and higher median serum levels of alanine aminotransferase and aspartate transaminase than did patients who were positive for enteroviruses. (cdc.gov)
  • We assessed differences between serum vitamin D levels and the risk of developing insulin resistance (interaction test), using a weighted multivariate logistic regression to analyze differences between participants with low and high magnesium intake levels. (frontiersin.org)
  • In addition to assessing genital and systemic safety, we determined TFV concentrations in plasma and cervicovaginal fluid (CVF) and LNG levels in serum using tandem liquid chromatography-mass spectrometry. (cdc.gov)
Quantification1
Elemental composition1
  • Mass spectrometry is used to determine the molecular mass, the elemental composition, structure and sometimes amount of chemical species in a sample by ionizing the analyte molecules and observing their behavior in electric and magnetic fields. (newworldencyclopedia.org)
Detector1
  • It has been widely observed that for many analytes, at high concentrations, ESI exhibits a loss of detector response linearity, perhaps due to reduced charge excess caused by analyte saturation at the droplet surface, inhibiting subsequent ejection of gas phase ions from further inside the droplet. (wikipedia.org)
Indications1
  • Since standard compounds were not available, they were tentatively identified by overlapping various analytical approaches, in agreement with the indications recommended in mass spectrometry (MS)-based metabolomics. (unicatt.it)
Analytical3
  • Mass spectrometry and high-performance liquid chromatography are the analytical techniques used in chemistry and biology for identifying the components of the sample, which use different approaches. (studykraken.com)
  • To understand the advantages of the use of mass spectrometry with high-performance liquid chromatography, it is important to check the main limitations which can be observed in each of these analytical techniques if used separately. (studykraken.com)
  • Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , 845 (1), 69-73. (elsevierpure.com)
Matrices1
  • Biological sample matrices naturally tend to contain many endogenous species with high basicity and surface activity, hence the total concentration of these species in the sample will quickly reach levels at which ion suppression should be expected. (wikipedia.org)
Ionization4
  • In particular, we utilized matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to spatially characterize lipidome changes. (nature.com)
  • A paper spray ionization mass spectrometry (PSI-MS) was developed to detect and quantify bisphenol S in three different brands of thermal papers commercially available. (springer.com)
  • Chen S, Chang Q, Yin K, He Q, Deng Y, Chen B, Liu C, Wang Y, Wang L (2017) Rapid analysis of bisphenol a and its analogues in food packaging products by paper spray ionization mass spectrometry. (springer.com)
  • Currently, a form of mass spectrometry called matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) is being used to identify bacteria (including mycobacteria), yeasts, molds, and potentially viruses. (msdmanuals.com)
Method2
Detect1
  • The main limitation of performance liquid chromatography is that this technique is unable to detect the components of the mixture definitely. (studykraken.com)
Ionic1
Identification3
Chromatographic1
  • Sample preparation of biological samples and optimisation strategies for reversed-phase liquid chromatographic applications including selection of column and mobile phase compositions. (lu.se)
Analysis2
  • Advances in carotenoid analysis: Electrospray liquid chromatography-mass spectrometry using C30 reversed phase HPLC. (oregonstate.edu)
  • Applications of fast atom bombardment mass spectrometry (FAB-MS) and continuous-flow FAB-MS to carotenoid analysis. (oregonstate.edu)
Instrumentation1
  • Opposing to the traditional high performance liquid chromatography, its instrumentation in the combination with mass spectrometry is based on the smaller scales used for both diameters internal one and flow rate. (studykraken.com)
Composition1
  • L'objectif de ce travail était d'étudier la composition des graines de P. vulgaris afin d'en déterminer non seulement le potentiel nutritionnel (teneur en lipides, protéines, minéraux), mais aussi la présence de métabolites spéciaux et d'en déterminer les pouvoirs antioxydant et anti-inflammatoire. (ac.be)
Accurate2
  • TPs of BMPA were identified by fragmentation patterns and accurate mass measurements. (unito.it)
  • Accurate mass and isotopic pattern, MS/MS fragmentation, correlation between fragments observed and putative structures and between liquid chromatography coupled with mass spectrometry (LC/MS) and gas chromatography/ mass spectrometry signals were studied. (unicatt.it)
Laboratory2
  • To make it clear, high-performance liquid chromatography is aimed at separating the individual components of the mixture by means of a laboratory technique called chromatography, whose main principle is to measure an analyte from two phases of molecules, mobile and stationary. (studykraken.com)
  • Laboratory team performance is monitored using several techniques. (cdc.gov)
Spectra2
  • TGs containing Delta5-UPIFAs have a higher retention in comparison with common TGs found in plant oils with single methylene interrupted Delta6(9)-FAs and also significantly changed relative abundances of fragment ions in APCI mass spectra. (unboundmedicine.com)
  • The identities of the latter three major 2-glucosyloxycinnamic acids were confirmed by comparing their retention times, UV and mass spectra with those of the reference standards. (edu.hk)
Analyte4
  • Although the precise chemical and physical factors involved in ion suppression are not fully understood, it has been proposed that basicity, high concentration, mass and more intuitively, co-elution with the analyte of interest are factors which should not be ignored. (wikipedia.org)
  • A higher analyte/matrix ratio can give a reduced effect of ion suppression. (wikipedia.org)
  • At the same time, mass spectrometry can fail to identify the components of a mixture if the analyte of interest is considered to be part of the mixture. (studykraken.com)
  • There is a possibility to lose the analyte while extraction, electrospray is not always available, the sensitivity may depend on a number of factors, the problems may appear with chromatography if the stages of the procedure are confused, and the matrix is added to the mobile phase before the column (Faust 2005). (studykraken.com)
Concentrations1
  • High concentrations of interfering components give rise to an increased surface tension and viscosity, giving a reduction in desolvation (solvent evaporation), which is known to have a marked effect of ionisation efficiency. (wikipedia.org)
Study2
  • This study aimed to explore the metabolic effects of two carbohydrate sources with different degrees of risk in obesity development when associated with a high fat intake. (mdpi.com)
  • To derive the cutoff values and study the performance characteristics of salivary cortisol and salivary cortisone in the diagnosis of adrenal insufficiency. (medscape.com)
Methods1
  • Methods: The transformation pathways of BMPA were investigated using high-performance liquid chromatography (HPLC) combined with a hybrid LTQ-Orbitrap instrument on the basis of mass defect filtering. (unito.it)
Journal3
Spectrom3
Ultra-high1
Sample3
Research1
  • Feature papers represent the most advanced research with significant potential for high impact in the field. (mdpi.com)
Compounds3
  • In contrast, the traces for the other two filter types show that both have contributed leached compounds into the matrix, with knock-on effects on the mass spec results. (pall.com)
  • Despite dramatically higher drug plasma levels after TC compared with curcumin gavage, resulting brain levels of parent compounds were similar, correlating with reduction in LPS-stimulated inducible nitric-oxide synthase, nitrotyrosine, F2 isoprostanes, and carbonyls. (aspetjournals.org)
  • Omega-3 rich oil and an interesting amino acids profile were detected in the seeds as well as the presence of a high content of polyphenolic compounds, including two structurally new compounds (phenylpropane derivatives) which were isolated and structurally characterized. (ac.be)
Validation1
  • Assay validation for three antidepressants in pharmaceutical formulations: Practical approach using capillary gas chromatography. (scielo.br)
Content2
  • Content of 4-methoxyglucobrassicin (4-MGBS) and neoglucobrassicin (NGBS), on the other hand, were higher in chamber condition. (usda.gov)
  • Conclusions: The highest content of bioactive components was obtained from dried blossoms of Sambucus nigra L. for infusions with a total contact time of 30 minutes and for decoctions at a contact time of 45 minutes. (researchgate.net)
Products1
Levels3
  • The overexpression of α-synuclein via AAV lead to more severe dopaminergic degeneration and higher p-Ser129 α-synuclein levels in cPSAP DAT mice compared to WT mice. (nature.com)
  • Brassica vegetables contain many health-enhancing components, such as high levels of polyphenols and glucosinolates (GSLs). (usda.gov)
  • Homocystine metabolism is dependent on pyridoxine, and high homocystine levels can result from pyridoxine deficiency. (medscape.com)
Antioxidant1
  • Results: The infusions of fresh blossoms and fresh leaves of Sambucus nigra L had the highest antioxidant activity at the total contact time of 30 minutes (82.7 mmol TE/100 ml) and 35 minutes (36.5 mmol TE/100 ml), respectively. (researchgate.net)
Activity2
  • Interestingly, antibodies from monkeys vaccinated with the AMA1-RON2L complex had significantly higher neutralizing activity than antibodies from monkeys vaccinated with AMA1 alone. (nature.com)
  • Importantly, we show that antibodies from animals vaccinated with the complex have significantly higher neutralization activity against non-vaccine type parasites. (nature.com)