High malignant cloning clone cells. Medical search. Frequent questions

Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. (sigmaaldrich.com)
Six diatom species displayed specific anti-inflammatory, anticancer (blocking human melanoma cell proliferation), and anti-biofilm (against the bacteria Staphylococcus epidermidis ) activities whereas, none of the other microalgae were bioactive against the conditions tested for. (frontiersin.org)
Overexpression of SLAMF1 or SLAMF7 in IGHV mutated CLL cell models resulted in reduced proliferation and impaired responses to BCR ligation, whereas the knockout of both receptors showed opposing effects and increased sensitivity toward inhibition of components of the BCR pathway. (nature.com)
B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
The proliferation and growth of PDAC cells were detected by Celigo analysis, MTT, and clone formation assay. (jcancer.org)
PYCR1 interference was able to inhibit cell proliferation and promote cell apoptosis of pancreatic cancer. (jcancer.org)
It is unclear whether intraclonal V gene diversification by somatic hypermutation, which is strictly dependent on the enzyme activation induced cytidine deaminase (AID), is restricted to the early phase of lymphoma clone expansion and later silenced, or whether it remains active throughout malignant proliferation. (bmj.com)

In hematology, plasma cell dyscrasias (also termed plasma cell disorders and plasma cell proliferative diseases) are a spectrum of progressively more severe monoclonal gammopathies in which a clone or multiple clones of pre-malignant or malignant plasma cells (sometimes in association with lymphoplasmacytoid cells or B lymphocytes) over-produce and secrete into the blood stream a myeloma protein, i.e. an abnormal monoclonal antibody or portion thereof. (wikipedia.org)
Plasma cells develop from B lymphocytes (i.e. (wikipedia.org)
B cells) which are stimulated to undergo this maturational development by T lymphocytes during the latter cells' processing of these antigens. (wikipedia.org)
Lymphocytes do not show histologic atypia to suggest malignant transformation. (medscape.com)
Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)

These are independent clonal derivatives of a spontaneous malignant melanoma tumour in the mouse. (biologists.com)
To analyse whether AID is expressed in L&H cells as an indicator of active somatic hypermutation in the tumour cells. (bmj.com)

The relationship between the 'spontaneous' frequency of sister-chromatid exchanges (SCE) and tumorigenicity was studied in a series of hybrids between a C57BL melanoma cell line and diploid cells, but no correlation was found between the 2 variables. (biologists.com)

Multiple dominant clones can be detected by polymerase chain reaction (PCR) of T-cell receptor gene usage, which supports a reactive process. (medscape.com)
L&H cells from lymphocyte predominant HL cases and centroblasts from lymphadenites were micromanipulated and analysed for AID expression by quantitative real time polymerase chain reaction. (bmj.com)

this disorder is a form of plasma cell dyscrasia in which no myeloma protein is detected in serum or urine (at least as determined by conventional laboratory methods) of individuals who have clear evidence of an increase in clonal bone marrow plasma cells and/or evidence of clonal plasma cell-mediated tissue injury (e.g. plasmacytoma tumors). (wikipedia.org)
Two indicators of clonal complexity were used, namely, the number of foster clones, described as clones with similar D-N 2 -J rearrangements but other V-rearrangement and N 1 -joining, and the maximum across all foster clones of the number of evolved clones from one foster clone. (frontiersin.org)
1. IgH high-throughput sequencing allows new insights into the clonal architecture of BCP-ALL. (frontiersin.org)
It is a clonal genetic heterogeneous disease generally thought to arise from the malignant transformation and expansion of a single lymphoid progenitor at various stages of development ( 3 - 5 ). (frontiersin.org)

As the nomenclature and description of the disease spectrum under the descriptive term parapsoriasis evolved, the primary focus has been on the distinction of whether the disorder progresses to mycosis fungoides (MF) or cutaneous T-cell lymphoma (CTCL). (medscape.com)
The initiating cause of parapsoriasis is unknown, but the diseases likely represent different stages in a continuum of lymphoproliferative disorders from chronic dermatitis stimulated by activated T cells to frank malignancy of cutaneous T-cell lymphoma (CTCL). (medscape.com)
Early patch-stage cutaneous T-cell lymphoma. (medscape.com)

The spontaneous immortalization of primary malignant cells is frequently assigned to their genetic instability during in vitro culturing. (biomedcentral.com)

Cell lines from various tumor types have been developed for use as a tumor model for controlled laboratory studies. (biomedcentral.com)
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignant tumor with an extremely poor prognosis in digestive tumors. (jcancer.org)
Furthermore, the effect of PYCR1 interference on tumor growth was evaluated in vivo through injecting tumor cells subcutaneously into nude mice. (jcancer.org)
Pancreatic cancer is a highly malignant tumor with poor diagnosis in the digestive system. (jcancer.org)

The in vitro culture was established with cells isolated from ascites of a 60-year-old female patient with recurrent ovarian cancer. (biomedcentral.com)
The problem we have been concerned with is to find an in vitro correlate with tumorigenicity in a system of cell hybrids in the mouse. (biologists.com)

Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)

At the other end of this spectrum, detection of the myeloid protein is due to a hematological malignancy, i.e. multiple myeloma, Waldenström macroglobulinemia, or other B cell-associated neoplasm, that has developed, often in a stepwise manner, from their MGUS precursors. (wikipedia.org)
Hybrids in which malignancy was suppressed and malignant segregants derived from them showed virtually identical SCE frequencies. (biologists.com)

11 To clarify this issue for lymphocyte predominant HL we quantitatively examined the expression of AID in the L&H cells of six patients with lymphocyte predominant HL at the transcription level, because suitable antibodies for immunohistochemistry are not yet available. (bmj.com)

Peripheral T cell lymphomas (PTCLs) are generally chemotherapy resistant and have a poor prognosis. (mdpi.com)

Immunoglobulin Heavy Chain High-Throughput Sequencing in Pediatric B-Precursor Acute Lymphoblastic Leukemia: Is the Clonality of the Disease at Diagnosis Related to Its Prognosis? (frontiersin.org)
3. Patients with a higher number of foster clones were patients in the better prognosis group. (frontiersin.org)

Genotypic pattern observed in small plaque parapsoriasis is similar to that observed in chronic dermatitis, and the pattern of clonality of T cells is consistent with the response of a specific subset of T cells that have been stimulated by an antigen. (medscape.com)
We identified a subset of Chronic Lymphocytic Leukemia (CLL) patients with high Signaling Lymphocytic Activation Molecule Family (SLAMF) receptor-related signaling that showed an indolent clinical course. (nature.com)

These genomic changes can go awry by placing a gene that controls cell growth an/or survival adjacent to a normally highly active antibody gene promoter and/or by causing the formation of extra chromosomes (see trisomy) or chromosomes with large deletions that result in the overexpression or under-expression, respectively, of genes that control cell growth and/or survival. (wikipedia.org)
Southern blot analysis of T-cell receptor genes from parapsoriasis does not identify a dominant clone of T cells. (medscape.com)

Indeed, our experiments showed significantly decreased degranulation capacity of primary NK cells from CLL patients expressing low levels of SLAMF1 and SLAMF7. (nature.com)
The maximum number of evolved clones was significantly higher in patients with t (12;21)/ ETV6:RUNX1 . (frontiersin.org)

The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)

Its lethality is a consequence of the lack of symptoms or biomarkers enabling the early diagnosis of disease, and the propensity of malignant cells to seed the peritoneal cavity. (biomedcentral.com)
Meanwhile, a high mortality rate is due to late diagnosis. (jcancer.org)
2. A higher number of evolved clones at diagnosis of BCP-ALL was associated with the presence of t (12;21)/ ETV6:RUNX1 . (frontiersin.org)

Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)

As they are stimulated to become plasma cells, B cells refashion parts of their genome in efforts to create a new gene that encodes a functional antibody. (wikipedia.org)
Eventually he cloned the GFP gene, but gave up on work to express the gene in mammalian cells. (pharmaceuticalintelligence.com)
The lymphocytic and histiocytic (L&H) cells of lymphocyte predominant Hodgkin lymphoma (HL) originate from germinal centre B cells and carry mutated V gene rearrangements, usually with intraclonal diversity. (bmj.com)
1 Analysis of V gene rearrangements in single L&H cells revealed the presence of somatic mutations with intraclonal diversity in functional monoclonal V gene rearrangements, in line with a GC B cell origin. (bmj.com)
7 Several studies analysing AID expression and mutation patterns in V gene rearrangements in various B cell non-HLs showed that, although somatic hypermutation activity is strictly dependent on AID, the expression of this protein does not necessarily indicate ongoing somatic hypermutation. (bmj.com)
Intraclonal diversity in V gene rearrangements as a result of active somatic hypermutation is a typical feature of GC B cells. (bmj.com)

Since the SLAMF low signature was strongly associated with an unmutated CLL immunoglobulin heavy chain (IGHV) status in large datasets, we investigated the impact of SLAMF1 and SLAMF7 on the B cell receptor (BCR) signaling axis. (nature.com)

10] Nevertheless, a hint to the verity of this hypothesis is the recent identification of increased telomerase activity in T cells from CTCL at low-grade stages, high-grade lymphoma, and parapsoriasis, which is activity not exhibited in normal T cells. (medscape.com)
Among the changes to CTCL classification were the addition of primary cutaneous acral CD8 + T-cell lymphoma as a new provisional entity. (medscape.com)

Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)

and may accumulate "secondary genomic changes" that cause them to become malignant. (wikipedia.org)
Additionally, comparative genomic hybridization was employed to compare genomic alterations between the CAISMOV24 cell line and its primary malignant cells. (biomedcentral.com)
Although the cell line had developed alongside the accumulation of genomic alterations (28 CNV in primary cells and 37 CNV in CAISMOV24), most of them were related to CNVs already present in primary malignant cells. (biomedcentral.com)

The first human, trophoblastic endocrine cell type to be maintained in continuous culture. (sigmaaldrich.com)

However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)

In addition, for three diatoms we also tested two different clones to disclose diversity in clone bioactivity. (frontiersin.org)

Furthermore, none of the 6 diatom species tested were toxic on normal human cells. (frontiersin.org)
Culturing conditions (i.e., nutrient starvation conditions) greatly influenced bioactivity of the majority of the clones/species tested. (frontiersin.org)
In summary, Dr. Prather had been working at Wood's Hole in Massachusetts trying to discover, isolate, then clone the protein which allowed a species of jellyfish living in the cold waters of the North Pacific, Aequorea victoria , to emit a green glow. (pharmaceuticalintelligence.com)

A previous study demonstrated that SLAMF1, SLAMF2, and SLAMF7 receptors are rather downregulated on CLL cells as compared to their normal B cell counterpart [ 12 ], suggesting that high expression of these molecules may have detrimental (e.g., antiproliferative) effects in the CLL context. (nature.com)
The use of cell lines as "biological minipumps" to chronically deliver antinociceptive molecules into the pain-processing centers of the spinal cord represents a newly developed technique for the treatment of pain [ 3 ]. (hindawi.com)
The CAISMOV24 line was assessed for cell growth, production of soluble biomarkers, expression of surface molecules and screened for typical mutations found in serous ovarian carcinoma. (biomedcentral.com)
The cell surface molecules EpCAM, PVR and CD73 are overexpressed on CAISMOV24 cells compared to the primary malignant cells. (biomedcentral.com)

B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most common pediatric neoplasm ( 1 , 2 ). (frontiersin.org)

Additionally, CAISMOV24 cell line was characterized as a low-grade serous ovarian carcinoma, which still resembles its primary malignant cells. (biomedcentral.com)
However, cell lines are known for their susceptibility to genetic and metabolic alterations, which can alter their characteristics in relation to the primary malignant cells. (biomedcentral.com)
Ideally, the more a cell line resembles the primary malignant cells, the more useful it will be for scientific research. (biomedcentral.com)

CAISMOV24 cell line harbored KRAS mutation with wild type TP53 , therefore it is characterized as low-grade serous carcinoma. (biomedcentral.com)

In this study, the new epithelial ovarian cancer cell line CAISMOV24 was described and compared with its original low-grade serous ovarian carcinoma. (biomedcentral.com)
One such guest was one of my colleagues Dr. Leonard Cohen, who worked with Dr. Irwin Rose and Avram Hershko at the Institute for Cancer Research in Philadelphia a cell-free system from clams to discover the mechanism how cyclin B is degraded during the exit from the cell cycle ( from A. Hershko's Nobel speech ). (pharmaceuticalintelligence.com)

Here, a clone of plasma cells refers to group of plasma cells that are abnormal in that they have an identical genetic identity and therefore are descendants of a single genetically distinct ancestor cell. (wikipedia.org)

A great many noxious agents capable of inducing SCE in tissue culture cells are carcinogenic (see, for example, Wolff, 1977 ). (biologists.com)

Somatic hypermutation may remain active in L&H cells in a considerable proportion of cases, increasing the risk of acquiring further transforming mutations. (bmj.com)

High-throughput sequencing (HTS) of the immunoglobulin heavy chain ( IgH ) locus is a recent very efficient technique to monitor minimal residual disease of B-cell precursor acute lymphoblastic leukemia (BCP-ALL). (frontiersin.org)

The lymphocytic and histiocytic (L&H) cells of lymphocyte predominant HL express B cell markers and also the germinal centre (GC) marker BCL6, indicating that they are derived from GC B cells. (bmj.com)

Plasma cells are key effector elements of the adaptive immune system. (wikipedia.org)
Together, our data indicate that SLAMF receptors are important modulators of the BCR signaling axis and may improve immune control in CLL by interference with NK cells. (nature.com)
Signaling Lymphocytic Activation Molecule Family (SLAMF) receptors are a group of nine type I transmembrane receptors that are mainly expressed on a variety of immune cells. (nature.com)

A lower number of foster clones was associated with a higher risk group after prephase and t (12;21)/ ETV6:RUNX1 genetic type. (frontiersin.org)

El-Darouti et al reported on a 7-year study of a hypopigmented disorder that the researchers believe should be classified as a new variant of parapsoriasis en plaque. (medscape.com)

1] With the range of histology detected, treatments are variable but therapies that affect T cells can be effective. (medscape.com)

RNA interference was used to inhibit the expression of PYCR1 in PANC- 1 and AsPC-1 cells. (jcancer.org)

this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)

Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner. (hindawi.com)
The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain. (hindawi.com)

Feature papers represent the most advanced research with significant potential for high impact in the field. (mdpi.com)

6 Recent studies of AID protein expression using western blot analysis showed that AID expression is largely restricted to normal and transformed GC B cells and correlates well with the expression of AID transcripts. (bmj.com)

These findings suggest that cell fusion might have induced epigenetic SCE frequency changes possibly in the same way as modulation of SCE frequencies is known to occur in the human leukocyte series. (biologists.com)

It has also been used to study placental transfer of highly purified non-dioxin-like polychlorinated biphenyls in BeWo cells. (sigmaaldrich.com)

Current terminology of parapsoriasis refers to 2 disease processes that are caused by T-cell-predominant infiltrates in the skin. (medscape.com)

Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. (sigmaaldrich.com)

They are known to be involved in the regulation of NK and T cell responses, mostly by homotypic interactions except for SLAMF2 (CD48) and SLAMF4 that interact with each other [ 10 ]. (nature.com)

The AID transcription level was higher than background in three of the six lymphocyte predominant HL cases, although it was lower than that seen in centroblasts. (bmj.com)

The overproduced monoclonal proteins, termed myeloma proteins, commonly circulate in blood, may accumulate in urine, and are the hallmarks of plasma cell dyscrasias including their most malignant forms viz. (wikipedia.org)
It has been suggested that sister-chromatid exchange (SCE) might reflect a pathway by which cells can repair certain forms of chromosome damage( Latt & Juergens, 1977 ). (biologists.com)

Although the development of research tools, whether imaging tools, vectors, animal models, cell lines, and such are not heralded, they always assist in the pivotal discoveries of our time. (pharmaceuticalintelligence.com)

multiple myeloma, light chain multiple myeloma, and plasma cell leukemia. (wikipedia.org)

In B cells, this class of receptors has been shown to be expressed in distinct patterns associated with development and activation [ 11 ]. (nature.com)

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