• Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. (wikipedia.org)
  • Results obtained using the FPIA method described earlier were compared to results obtained using high performance liquid chromatography (HPLC) with fluorometric detection at 385 nm excitation and 515 nm emission. (cdc.gov)
  • Immunoassays are used in most screening processes because they allow for large-scale screening and quick detection (Reference Armbruster, Schwarzhoff, and HubsterArmbruster 1993). (icg-12.org)
  • 1514 Eisenhower Place Ann Arbor, MI 48108-3284, USA Arbor Assays builds the highest quality detection and immunoassay products for clinically important biomolecules. (contractresearchmap.com)
  • The antibody detection method - enzyme-linked immunosorbent assay (ELISA) is especially relevant when examining imported unvaccinated livestock. (journalveterinariya.ru)
  • The assay detected signal across at least 3 orders of magnitude of lysate input with a linear range spanning 1-2 orders and a low femtomole limit of detection for EGFR phosphotyrosine. (biomedcentral.com)
  • The obvious benefit of this method is that the low sample requirement allows detection of SH2 binding in samples which are difficult to analyze using traditional protein interaction assays. (biomedcentral.com)
  • Its fluorescence detection system uses deep blocking filters and dichroic mirrors for the best level of performance in research and screening applications. (psu.edu)
  • Find an extensive collection of application notes, research, and detection technology related to microplate reader assays and applications including ELISAs, western blots, and protein and nucleic acid detection. (moleculardevices.com)
  • AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies. (stratech.co.uk)
  • Short- and long-range FRET co-functionalized fluorescence quenching collapsar probe regulates amplified and accelerated detection of Salmonella. (translationandethics.com)
  • Beckman Coulter Life Sciences' Valita Titer assays range are rapid, high throughput IgG quantification assays which use fluorescence polarization (FP) for detection. (mybeckman.ca)
  • Our robust and High-end microplate readers range from Single-detection mode plate readers up to Multi-mode plate readers, with up to seven detection modes. (isogen-lifescience.com)
  • In particular, we are working on biosensors based upon photonic crystal concepts that can either be built from low-cost flexible plastic materials, or integrated with semiconductor-based active devices, such as light sources and photodetectors, for high performance integrated detection systems. (illinois.edu)
  • High Resolution Label-Free Detection Applied to Microarray Research," L. Laing and B.T. Cunningham, Functional Protein Microarray in Drug Discovery, edited by P.F. Predki, CRC Press, p. 217-236, 2007. (illinois.edu)
  • ELISA Radio immunoassay FRET Magnetic immunoassay Fluorescence Immunoscreening Lateral flow test Cloned enzyme donor immunoassay Surround optical fiber immunoassay Plate reader Nielsen K, Lin M, Gall D, Jolley M (September 2000). (wikipedia.org)
  • The OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals recommends the use of four serological tests to rule out brucellosis in international animal trade: the buffered Brucella antigen tests (rose bengal test and buffered plate agglutination test), the complement fixation test (CFT), the enzyme-linked immunosorbent assays (ELISA) and fluorescence polarization assay (FPA). (journalveterinariya.ru)
  • Enzyme-linked immunosorbent assay (ELISA) was for IL-6 levels in peritoneal fluid and cell culture supernatant. (endometriosi.it)
  • Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)
  • The Synergy 2 is controlled by BioTek's Gen5™ Reader Control and Data Analysis Software, is certified for HTRF®, DLR™ and LanthaScreen™, and is compatible with AlphaScreen assays. (psu.edu)
  • Here a microplate reader equipped with a flash lamp is used to detect ERK phosphorylation using an AlphaScreen ® SureFire ® proximity-based assay. (bmglabtech.com)
  • The AlphaScreen ® assay uses the diffusion of singlet state oxygen from Donor to Acceptor beads. (bmglabtech.com)
  • The AlphaScreen ® SureFire ® ERK1/2 phosphorylation assay is based on an immuno-sandwich assay principle (Figure 1). (bmglabtech.com)
  • In addition, the EMIT assays for MTX lack the sensitivity of reliable quantification to 0.05 μmol/L needed because of today's more rigorous requirements. (elsevierpure.com)
  • It enables researchers to further increase their throughput for IgG quantification, with the ability to analyze up to 4X the number of samples per plate, using 33% less sample volume, versus the traditional Valita Titer 96-well assay. (mybeckman.ca)
  • Microplate reader control and data analysis software with preconfigured protocols and custom assay workflow. (moleculardevices.com)
  • BMG LABTECH has developed a wide range of dedicated and multi-mode microplate readers for life sciences applications and high-throughput screening. (news-medical.net)
  • When a fluorescent-labelled molecule (tracer) binds to another molecule the rotational motion will change, resulting in an altered intensity of plane-polarized light, which results in altered fluorescence polarization. (wikipedia.org)
  • They include a fluorescence polarisation immunoassay, which uses a fluorescent dye to detect cortisol in water, and a labeled-antibody assay, which relies on antibodies bound to fixed cortisol. (charlesreedcagle.com)
  • The device has also shown a reliable fluorescent recognition of the assay. (news-medical.net)
  • With more than a decade of experience in this field, PerkinElmer provides a portfolio of high-content screening products - imaging instruments, software, automation, fluorescent probes and microplates - to help you answer complex biological questions. (gulf-scientific.com)
  • Our fluorescent agents and labels are optimized for use in the full range of PerkinElmer optical in vivo imaging systems, as well as other fluorescence microscopy systems and many in vitro and cell-based systems. (gulf-scientific.com)
  • FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays. (stratech.co.uk)
  • An important assay in our arsenal is the analysis of aggregation kinetics using fluorescent amyloid dyes. (isogen-lifescience.com)
  • Despite originating as a method for direct interaction studies, the technique has been adopted by high-throughput screening (HTS) since the mid 1990s to help facilitate the drug discovery process by studying complex enzymatic interaction. (wikipedia.org)
  • This feature along with short assay runtime makes this method a useful platform for the development of high throughput assays to determine modular domain-ligand interactions which could have wide-ranging applications in both basic and translational cancer research. (biomedcentral.com)
  • The Opera Phenix is designed for high-throughput, phenotypic screening and assays involving complex disease models, such as live cells, primary cells, and microtissues. (gulf-scientific.com)
  • Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
  • To realise optimal high throughput on the PHERAstar we use a stacker, which is ideal for the many samples we measure for our clients projects. (isogen-lifescience.com)
  • 6859 Quality Way Portage, MI 49002 Eurofins AvTech Labs offers a range of analytical and bioanalytical services including in vitro ADME assays, in vivo PK, bioanalysis, immunoassay development and biomarker ID and GMP method development. (contractresearchmap.com)
  • In future experiments, we seek to monitor the effects of TBI-induced injuries over a prolonged time and at a higher temporal resolution to better understand the subtleties of the biomarker release kinetics and the cell recovery phases. (hes-so.ch)
  • For over 5 years, we use the CLARIOstar plate reader successfully for our biomarker assay research of neurodegenerative diseases. (isogen-lifescience.com)
  • We provide these biomarker assays to CRO's and pharma companies. (isogen-lifescience.com)
  • Our results demonstrate that the asymmetric configuration, substituted by both the donor and acceptor on the diarylethene molecule, exhibits the highest switching ratio and rectification ratio. (bvsalud.org)
  • To measure the acceptor fluorescence gain was set using a sample with the highest acceptor dye and no donor, for example, well P3. (bmglabtech.com)
  • Similarly, gain for donor fluorescence measurement was set on well with highest donor dye and no acceptor dye, such as well D1. (bmglabtech.com)
  • Cloned enzyme donor immunoassay (CEDIA), enzyme-multiplied immunoassay method (EMIT), fluorescence polarization immunoassay, immunoturbidimetric assay, and radioimmunoassay are all immunoassay techniques. (icg-12.org)
  • From 1925 to 1926 Francis Perrin detailed a quantitative theory for fluorescence polarization in multiple significant publications which remain relevant to this day. (wikipedia.org)
  • An immunoassay was used for quantitative analysis of MTX levels. (lww.com)
  • There are three assay platforms for SH2 profiling: quantitative far-Western, rosette, and oligonucleotide-tagged multiplex (OTM) assays [ 10 ]. (biomedcentral.com)
  • Our in vitro studies included scratch wound-healing migration assays and transwell assays to examine PPI's effect on HCC cell migration and invasion. (bvsalud.org)
  • The SH2-PLA assay utilizes oligonucleotide-conjugated anti-GST and anti-EGFR antibodies recognizing a GST-SH2 probe and cellular EGFR, respectively. (biomedcentral.com)
  • Current enzyme multiplied immunoassay technique (EMIT) assays for caffeine, amikacin, and MTX lack low-end precision. (elsevierpure.com)
  • The goal of the present study was to optimize the EMIT method parameters on the Dimension RxL Max, thereby providing applications with improved precision for all the three analytes and enhancing the sensitivity of the EMIT methotrexate assay. (elsevierpure.com)
  • These EMIT method applications now compare well with other established assays. (elsevierpure.com)
  • We build robust immunoassay and enzyme activity kits to quantitate biomolecules in biological matrices, provide custom assay testing services, and offer world-class customer support. (contractresearchmap.com)
  • The Meso QuickPlex instrument is the newest multiplexing instrument from MSD, offering affordable access to high‑performance, electrochemiluminescence immunoassays. (psu.edu)
  • We showed for the first time that interactions between SH2 domain probes and EGFR in cell lysate can be determined in a microliter-scale assay using SH2-PLA. (biomedcentral.com)
  • The assays rely on interactions between a fluorescently labeled IgG Fc-specific probe and the Fc of an IgG. (mybeckman.ca)
  • Advanced instruments enable high resolution imaging of biochemical and cellular interactions with the ability to monitor images of biochemical interactions as a function of time. (illinois.edu)
  • The QuickPlex has a wide menu of commercially available assay kits and a full line of components and reagents for developing your own assays. (psu.edu)
  • Browse PerkinElmer's reagents for in vivo imaging solutions, as well as high-content screening analysis. (gulf-scientific.com)
  • PhenoVue™ cellular imaging reagents are designed to streamline your high-content imaging workflows. (gulf-scientific.com)
  • PerkinElmer's drug discovery reagents cover a wide array of applications ranging from immunoassays platforms using Alpha, TR-FRET, and TRF technologies, to kinase assays, reporter gene, cytotoxicity and cell proliferation assays. (gulf-scientific.com)
  • to a large decrease in measurement time and a higher sensitivity, resulting in the use of smaller amounts of reagents. (isogen-lifescience.com)
  • Using the sensors and instrumentation, we are exploring new applications for optical biosensor technology including protein microarrays, biosensor/mass spectrometry systems, and microfluidics-based assays using nanoliter quantities of reagents. (illinois.edu)
  • We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyrosine phosphorylation state of cells. (biomedcentral.com)
  • In-Cell Western assay was used for protein expression of CCL17 and phosphorylation levels of ERK, JNK, and P38. (endometriosi.it)
  • In addition to fabricating devices, our group is also focused on the design, prototyping, and testing of biosensor instrumentation for high sensitivity, portability, and resolution. (illinois.edu)
  • This two-stage amplification closed-tube diagnostic assay was used to test SARS-CoV-2 samples with significantly enhanced sensitivity compared to conventional RT-PCR with dynamic range of at least seven orders of magnitude (2 × 10 −4 -2000 TCID 50 /reaction) for RNA from SARS infected cells and below 10 copies per reaction for DNA plasmid as positive standards [ 1 ] . (encyclopedia.pub)
  • Non-fasting serum lipid levels were measured using the Cobas Integra 400 Plus Chemistry analyser, an automated analyser that employs four different technologies: absorption photometry, fluorescence polarization immunoassay, immune-turbidimetry, and potentiometry for accurate analysis. (pgscatalog.org)
  • The rotation of the molecules between absorption and emission of the photon has the effect of "twisting" the polarization of the light. (mybeckman.ca)
  • Within- and between-day imprecision were less than 6% for low to high concentrations of caffeine and amikacin controls and are less than 7.5% for MTX concentrations greater than 0.3 μmol/L and 12.3% at 0.06 μmol/L. The correlation coefficients for caffeine, amikacin, and MTX plotted for the Dimension RxL Max versus the methods used at Quest Diagnostics were 0.973, 0.986, and 0.992, respectively. (elsevierpure.com)
  • Complete solution of validation tools and services as well as a broad range of consumables and assays for all your lab needs. (moleculardevices.com)
  • Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the Avidin-biotin interaction to be used for the development of robust and highly sensitive assays. (genomicglossaries.com)
  • Easy-to-use, robust assay kits for life science research drug discovery and development, and bioassays. (moleculardevices.com)
  • Isogen Life Science is proud to distribute the robust, high quality BMG plate readers for already 20 years. (isogen-lifescience.com)
  • With the Real-Time Quaking Induced Conversion (RT-QuIC) assay we can detect aggregates of alpha-synuclein in cerebrospinal fluid of patients. (isogen-lifescience.com)
  • One limitation of current SH2 profiling methods is that these assays themselves do not provide the identity of the binding proteins. (biomedcentral.com)
  • Responses for many GPCRs such as Gi coupled GPCRs are not easily measured in current assay formats and other methods have been investigated as alternative readout. (bmglabtech.com)
  • The Synergy 2 is BioTek's high-performance multi-mode plate reader designed for the life science research and drug discovery markets. (psu.edu)
  • Valita Titer plate-based, 96- and 384-well assays that offers a rapid, cost effective way to measure IgG. (mybeckman.se)
  • Valita Titer is among the industry's fastest IgG tests and is compatible with all plate readers with a Fluorescence Polarization module. (mybeckman.se)
  • Figure 1 depicts the expected assay results for a 1:1 protein-protein interaction. (bmglabtech.com)
  • Be it in vivo imaging or high-content analysis, PerkinElmer's imaging solutions allow you to measure cellular and biological events, providing information rich datasets to streamline and accelerate basic research and drug discovery. (gulf-scientific.com)
  • Observing that polarization increased with viscosity of the solvent and the size of the dye molecule, but decreased with an increase in temperature, he deduced that polarization increased with a decrease in mobility of the emitting species. (wikipedia.org)
  • Cells were incubated for 18h and then the assay performed. (bmglabtech.com)
  • The CCL17-CCR4 axis between endometrial stromal cells and macrophages contributes to the high levels of IL-6 in ectopic milieu. (endometriosi.it)
  • The effects of AKBA on the cell viability in A549, H460, H1299, and BEAS-2B cells were determined by the CCK-8 assay. (boswellia.org)
  • Available for use in the BCL to read bead platform assays, the Bio-Plex® 200 uses Bio-Plex Manager™ Software to read both polystyrene and magnetic bead assays and can perform up to 100 different analytes in a single well. (psu.edu)
  • HPLC-FD, HPLC-ED, and GC/MS). Thus, the Abbott Homocysteine assay was used as the primary method for determination of plasma total homocysteine in NHANES 2003-2004. (cdc.gov)
  • Cell Meter™ cellular functional assay kits, a complete set of tools for functional analysis of cellular events. (stratech.co.uk)
  • The CLARIOstar is perfect due the flexibility of the monochromator, as we measure all kinds of samples and kits in different assays. (isogen-lifescience.com)
  • Operating under fixed temperature and viscosity allows for the fluorescence polarization to be directly proportional to the size of the fluorophore. (wikipedia.org)
  • This leads the crude oil viscosity of the system containing normal asphaltene is higher than that of the system containing acidic asphaltene under the same mass fraction (382 µ Pa·s for AAsp and 416 µ Pa·s for NAsp). (bvsalud.org)
  • From basic research to assay development and screening, PerkinElmer's high-content analysis products are designed to help you take your research further, in less time than ever before. (gulf-scientific.com)
  • Intravenous high-dose methotrexate (MTX ≥ 1 g/m 2 ) is frequently used in patients with cerebral lymphoma or other malignancies. (lww.com)
  • Trigging isothermal circular amplification-based tuning of rigorous fluorescence quenching into complete restoration on a multivalent aptamer probe enables ultrasensitive dete ction of Salmonella. (translationandethics.com)
  • The colony formation assay was used to identify the effects of AKBA on cell proliferation. (boswellia.org)
  • After intramuscular administration, high MTX concentrations are found in SM and cortical and trabecular bone of RA patients. (urosario.edu.co)
  • Its multiple capabilities include syringe pump and peristaltic pump dispensers for washing and dispensing in standard assays and vacuum filtration protocols on a single platform. (psu.edu)
  • Uncover deep biological understanding in your everyday assays and innovative applications using the Operetta CLS high-content screening analysis system. (gulf-scientific.com)
  • Although nanosized transition-metal-based oxides/hydroxides exhibit high catalytic activity toward the oxygen evolution reaction (OER), many of them suffer from low stability at an anode current density in industrial scale. (bvsalud.org)
  • Retrieved on December 11, 2023 from https://www.news-medical.net/whitepaper/20180614/Inhibition-of-Mitochondrial-Permeability-Transition-Pore-Evaluated-by-Calcium-Retention-Capacity-Assay.aspx. (news-medical.net)
  • Feature papers represent the most advanced research with significant potential for high impact in the field. (mdpi.com)
  • After adjustment, PCPs with high belief in evidence uncertainty were less likely (OR=0.19, 95% CI=0.06, 0.62) to offer PSA and more likely to practice shared decision making (OR=1.80, 95% CI=1.22-2.67). (cdc.gov)
  • This generic type of device includes immunoassays and chromatographic assays for tacrolimus. (medicregister.com)
  • Hence this type of assay has the ability to measure cellular changes activated by Gs, Gi and Gq coupled receptors giving potential to use a single assay format for screening. (bmglabtech.com)
  • The confocal scanning unit of the Opera Phenix™ High-Content Screening System provides an increased distance between pinholes to reduce the pinhole crosstalk, leading to improved 3D image quality. (gulf-scientific.com)
  • The Opera Phenix® high-content screening system is the premier confocal solution for the most demanding high content applications. (gulf-scientific.com)
  • Introduction An important yet understudied component of postpartum type 2 diabetes risk reduction among high risk women is experiences with the healthcare system. (cdc.gov)