• Acetyl- and butyrylcholinesterase (ACHE, BCHE) from evolutionarily distant species display a high degree of primary sequence homology and have biochemically similar catalytic properties, yet they differ in substrate specificity and affinity for various inhibitors. (inrae.fr)
  • A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
  • In another embodiment, the present invention relates to a gene comprising a DNA sequence having at least a portion of the nucleotide sequence set forth in SEQ ID NO: 1. (justia.com)
  • In one embodiment, the nucleic acid comprises a nucleotide sequence having at least a portion of the sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 41, and SEQ ID NO: 43. (justia.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • However, large-scale nucleotide sequencing directed at the human genome (3 billion nucleotides! (nationalacademies.org)
  • The derived amino sequence was found to contain a region of high homology (54%) to the rabbit skeletal muscle enzyme and also very significant homology (35%) to the catalytic subunit of phosphorylase b kinase and cGMP-dependent protein kinase. (torvergata.it)
  • 16S rRNA genes from different species have significant homology, but the gene also includes hypervariable regions that are generally speciesspecific, and are determined by the microbial composition of the community. (neb.com)
  • The completion of the sequencing of the human genome has uncovered 32,000 to 35,000 predicted genes, offering the prospect for many more drugable targets. (ddw-online.com)
  • The sequence analysis of the 32-35,000 predicted genes indicates the there may be as many as 1,000 kinase or kinase-like genes. (ddw-online.com)
  • These characteristics enable the use of universal primer pairs to amplify 16S genes from many organisms in the same PCR reaction and then, through subsequent sequencing of the PCR products, the individual species represented can be identified. (neb.com)
  • For any given set of species, gene families are created by first identifying all the genes in all species and then clustering them based on their sequence similarities. (biorxiv.org)
  • Dimerized STATs then translocate to the nucleus, where they bind DNA in the promoter sequences of target genes to activate transcription. (medscape.com)
  • The male sterilizing factors are produced by recombination of the mitochondrial genome and consist of unusual genes or open reading frames (ORFs) that usually contain a portion of functional mitochondrial genes and sequences of unknown origin ( Hanson and Bentolila, 2004 ). (frontiersin.org)
  • The env genes contain a cis-acting RNA target sequence for the rev protein (= GENE PRODUCTS, REV ), termed the rev-responsive element (RRE). (lookformedical.com)
  • 3 ] found that disease genes evolved with higher nonsynonymous/synonymous substitution rate ratios (Ka/Ks) than non-disease genes, but Huang et al. (biomedcentral.com)
  • Eisenberg and Levanon found that housekeeping genes were compact in their coding lengths, which could be the result of higher selective pressure[ 11 ]. (biomedcentral.com)
  • Studies of AMINO ACID SEQUENCE HOMOLOGY and NUCLEIC ACID SEQUENCE HOMOLOGY provide useful information about the genetic relatedness of genes, gene products, and species. (bvsalud.org)
  • Evolutionary events that change genomic sequences are often classified into small changes and large structural changes [ 6 ]. (biomedcentral.com)
  • Structural changes involve longer genomic segments, thereby affecting the structure and order of genomic sequences. (biomedcentral.com)
  • One could say the primary limitation to crop improvement is no longer, "can we make a precise sequence change in a precise location in eukaryotic genomic DNA? (frontiersin.org)
  • Other services include online platforms for comparative genomic analysis of multiple sequences obtained from metagenomic experiments using the 'next generation sequencing platforms' (e.g. (biomedcentral.com)
  • The GB hepatitis viruses (GBV-A, GBV-B and GBV-C). The GB hepatitis viruses were cloned recently and preliminary genomic characterization shows that they are related to other positive-stranded RNA viruses with local regions of sequence identity with various flaviviruses. (medmuv.com)
  • We demonstrate a robust high-throughput duplex TaqMan qPCR assay for identification of male-specific genomic signatures using a novel MADC2 qPCR probe. (cannaqa.wiki)
  • The highest degree of homology in the Cox 1 nucleic acid sequences and predicted amino acid sequences was found between A. agkistrodontis and A. armillatus . (unl.edu)
  • Comparison of amino acid sequences among MTGR1, MTG8, and Nervy revealed four evolutionarily conserved regions (NHR1 to NHR4). (embl-heidelberg.de)
  • Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
  • It possesses a universal sample-processing cartridge for conducting multiplexed assays, either nucleic acid or immunoassay-based, and is field deployable. (genengnews.com)
  • We also carried out assay development for high-resolution melting analysis (HRM), loop-mediated isothermal amplification (LAMP), and TwistDx recombinase polymerase amplification (RPA) assays using MADC2 for male identification. (cannaqa.wiki)
  • With these markers developed for high-throughput testing assays, the cannabis industry will be able to easily screen and select for the desired sex of a given cultivar depending on the application. (cannaqa.wiki)
  • In one embodiment, the present invention relates to a recombinant protein having a protein comprising at least a portion of the amino acid sequence set forth in SEQ ID NO:2. (justia.com)
  • In some embodiments, the present invention provides a protein which consists of the amino acid sequence shown in SEQ ID NO: 2 having a deletion, substitution, or addition of at least one amino acid and which has neurogenesis inducing activity. (justia.com)
  • The present invention also relates to a gene which hybridizes with the neurogenesis inducing gene encoding for a protein comprising at least a portion of the amino acid sequence set forth in SEQ ID NO:2. (justia.com)
  • In another embodiment, the present invention relates to an isolated nucleic acid encoding a protein having at least a portion of the amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 42 and in SEQ ID NO: 44. (justia.com)
  • The genetic relationships between 64 representative strains from Spain and other reported isolates from the United States, Germany, Italy, Japan, and Sweden were described by analyzing the partial sequence of the major capsid protein (VP1) gene. (cdc.gov)
  • Changes in EV13 coding sequence involved several differences in the C-terminal extreme of the VP1 protein. (cdc.gov)
  • Although five HEV species exist ( 2 ), they are grouped into four clusters (HEV-A, -B, -C, and -D) on the basis of sequence analysis of the major capsid protein (VP1) and the 3′ noncoding region ( 3 ). (cdc.gov)
  • To predict ARDs in the intestinal microbiota, we developed a method based on protein homology modelling (see Methods) that we termed pairwise comparative modelling (PCM). (nature.com)
  • Both of the cAMP-dependent protein kinase phosphorylation sites were identified by sequence homology. (torvergata.it)
  • The architecture of virions is composed of nucleic acid and nucleocapsid protein to form the helical nucleocapsid. (newvita.com)
  • Chemical shift changes upon the addition of single-stranded RNAs (ssRNAs) identified a group of residues that form a positively charged patch on the protein surface as the binding site responsible for the previously reported affinity for nucleic acids. (pdfroom.com)
  • or the transmembrane protein gene cassettes of some pathogenic bacteria ( Santoyo and Romero, 2005 ), gene conversion can also generate sequence diversity. (elifesciences.org)
  • In this work, we show that substrate recognition of Tsp is mediated by a PDZ domain, a small protein module that promotes protein-protein interactions by binding to internal or C-terminal sequences of their partner proteins. (embl.de)
  • The isolated PDZ domain (amino acids 206-334) is capable of folding into a well-behaved structure and binds to a nonpolar peptide with a dissociation constant (K(D)) of 1.9 microM, similar to that of the intact Tsp protein. (embl.de)
  • In serotype 4b, gtcA appears to be the first member of a bicistronic operon which includes a gene with homology to Bacillus subtilis rpmE , encoding ribosomal protein L31. (asm.org)
  • The PPR protein family is consequently divided in subfamilies depending on the number and type of repeats present in their sequence as well as optional C-terminal domains. (frontiersin.org)
  • While measured rates of gp32 binding and dissociation indicate nM binding affinity, at â ¼1000-fold higher protein concentrations gp32 continues to bind into and restructure the gp32-ssDNA filament, leading to an increase in its helical pitch and elongation of the substrate. (bvsalud.org)
  • In doing so, this GEF-facilitated exchange of GDP for GTP is associated with structural changes in the GTP-binding protein which influence the degree to which the GTP-binding protein can interact with other molecules. (justia.com)
  • This invention provides an APP-cleaving protein and related nucleic acid compounds. (everypatent.com)
  • Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
  • These ring helicases bind single stranded nucleic acid through their central channel and their subunits display a high degree of cooperativity, which is among the topic of our studies. (rutgers.edu)
  • Specificity takes into account the homology of various primers and probes for the many different HPV genomes, given that there may be numerous species that share some of these sequences. (genengnews.com)
  • Sequence comparison through multiple alignment is an indispensable tool for understanding genomes and their shared histories [ 1 ]. (biomedcentral.com)
  • Non-colinear alignments, as opposed to colinear alignments, model all kinds of evolutionary changes and thereby enable correct homology prediction for whole genomes with non-colinear changes. (biomedcentral.com)
  • Over and above, non-colinear multiple global alignments of whole genomes, genome alignments for short, integrate as much sequence similarity information as is available. (biomedcentral.com)
  • Together with the prediction of homology, genome alignments provide a segmentation of the genomes originating from large structural changes. (biomedcentral.com)
  • This endeavour holds particular promise for translational medical microbiology, where a potentially unlimited number of sequenced microbial genomes might be coupled with the breadth of infectious diseases literature. (biomedcentral.com)
  • Mumame is not aiming to find strain-level differences in taxonomic composition, thus enabling it to operate at much lower sequencing depths as complete coverage of the targeted genomes is not necessary for the analysis. (pensoft.net)
  • Recent advances in rapid, low-cost sequencing have opened up the opportunity to study complete genome sequences. (biomedcentral.com)
  • Here, we present 20 draft genome sequences of Clostridium botulinum type A isolates originating from foodborne outbreaks in the United States and Ethiopia. (cdc.gov)
  • The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)
  • Accordingly, the present invention envisions an isolated nucleic acid encoding the GEF-H1S polypeptide of SEQ ID NO:2, as well as an isolated nucleic acid comprising the sequence of SEQ ID NO:1. (justia.com)
  • In this context, the invention also contemplates an isolated GEF-H1S polypeptide comprising the sequence of SEQ ID NO:2. (justia.com)
  • In a preferred embodiment, a GEF-H1 polypeptide is provided that lacks the amino acid region between residues 162 and 354 of SEQ ID NO. 2. (justia.com)
  • When antibodies, small molecules, or nucleic acids bind to the surface they affect the current flow, which is recorded as a change in impedence in the chip that carries the sample. (genengnews.com)
  • IRF-3 is constitutively expressed in most cell types and becomes phosphorylated following the recognition of a variety of pathogen-associated molecular patterns (PAMPs) (33) including viral nucleic acids (74). (health-ground.com)
  • DNA sequences that form the coding region for the viral envelope (env) proteins in retroviruses. (lookformedical.com)
  • The introduction of solid supports for DNA hybridisation/reassociations greatly broadened the range of applications of nucleic acid hybridisations, and provided the basis for solid-based methods being used today. (ddw-online.com)
  • Understanding the molecular basis of higher brain functions is important, not only to elucidate the universal principle of these processes, but also in the development of new therapeutic methods for treatment of diseases involving brain functions (i.e., neurogenesis). (justia.com)
  • The advent of nucleic acid amplification methods has facilitated the study of the molecular epidemiology of HEV ( 4 - 6 ). (cdc.gov)
  • Several reverse transcription-polymerase chain reaction (RT-PCR) methods were developed for this purpose, most of which analyze different sequence fragments within the VP1 gene ( 7 - 9 ). (cdc.gov)
  • Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
  • As described in Materials and Methods, we constructed a cDNA library from fat body tissues of E. coli - challenged A. mylitta larvae and randomly sequenced a large number of inserts from the library. (biomedcentral.com)
  • In previous studies, traditional PCR and non-high-throughput methods have been reported for the detection of male cannabis, and in our study, we present multiple methodologies that can be carried out in high-throughput commercial cannabis testing. (cannaqa.wiki)
  • However, attempts to quantify point mutations in metagenomic sequencing data often go wrong because the methods do not distinguish sufficiently well between mutated and wildtype variants of the same gene. (pensoft.net)
  • More than 60 percent of all National Institutes of Health grants-covering a wide range of problems in medicine and biology-propose use of the molecular methods of cloning, mapping, or sequencing to investigate problems. (nationalacademies.org)
  • Here, we address this problem by providing a software tool called Mumame, which can distinguish between wildtype and mutated sequences in shotgun metagenomic data and quantify their relative abundances. (pensoft.net)
  • One of the most appealing targets for collaboration has been whole genome analysis, especially the linkage of new sequencing data with the wealth of unstructured information captured in biomedical literature. (biomedcentral.com)
  • Nineteen C. botulinum type B isolates from Hawaii and one type B isolate from California were analyzed by whole-genome sequencing. (cdc.gov)
  • In another embodiment, serine-67 is present in a peptide of less than 30 amino acids that comprises the sequence of SEQ ID NO. 4. (justia.com)
  • 1. A purified protease which comprises the amino acid sequence SEQ ID NO:1, or a functional equivalent thereof produced by conservative amino acid alterations which result in a protease which exhibits the same biological, biochemical, chemical, physical and structural properties of the protease of SEQ ID NO:1. (everypatent.com)
  • Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
  • The degree of homology between target and probe results in stable hybridization. (enzolifesciences.com)
  • The RNA polymerases encoded by certain bacteriophages have a simple and economical organization, Interestingly, these polymerases show homology to mitochondrial and chloroplast RNA polymerases. (rutgers.edu)
  • Following duplication events, the resulting stretches of homologous sequence can promote recombination between gene copies. (elifesciences.org)
  • Genome alignments, which are the focus of this article, integrate more information than rearrangement studies by combining segmentation and sequence similarity. (biomedcentral.com)
  • The degree of similarity between sequences. (bvsalud.org)
  • The sequence and structure prediction of CCCVd has been documented. (wikipedia.org)
  • This is comparable to the way global alignments integrate more information than local alignments by assigning all parts of sequences to each other, and the way multiple alignments take information from more than two sequences into account for homology prediction. (biomedcentral.com)
  • The goal is homology prediction instead of reconstruction of evolutionary histories. (biomedcentral.com)
  • For this purpose, we developed and validated an annotation method (called pairwise comparative modelling) on the basis of a three-dimensional structure (homology comparative modelling), leading to the prediction of 6,095 ARDs in a catalogue of 3.9 million proteins from the human intestinal microbiota. (nature.com)
  • We describe the molecular epidemiology of the EV13 isolates obtained in Spain from the 2000 outbreak by analyzing the VP1 partial sequences of 64 identified viruses. (cdc.gov)
  • Molecular biologists have made many important theoretical advances from such comparisons, notably elucidation of the central role of DNA (deoxyribonucleic acid) as the carrier of genetic information in all free-living organisms. (nationalacademies.org)
  • 1995). Diisocyanates and acid anhydrides cyanates has a low association with atopy, low prevalence are among the most common low molecular weight (LMW) with specific IgE antibodies and a mixed Th1-Th2 response classes of chemicals responsible for occupational asthma. (cdc.gov)
  • The symptoms of LMW asthma are similar to those induced allergens like trimellitic anhydride (TMA), the relationship by high molecular weight (HMW) compounds. (cdc.gov)
  • Random priming is a type of primer extension in which a mixture of small oligonucleotide sequences, acting as primers, anneal to a heat-denatured double-stranded template. (enzolifesciences.com)
  • In another embodiment, an isolated nucleic acid encoding the GEF-H1 peptide of SEQ ID NO:3 is provided. (justia.com)
  • In similar fashion, one other embodiment provides an isolated nucleic acid encoding the GEF-H1 peptide of SEQ ID NO:4. (justia.com)
  • In another embodiment, a peptide consisting essentially of the sequence of SEQ ID NO. 4 is also provided. (justia.com)
  • Thus, in one embodiment, peptides comprising the sequences described in SEQ ID NOs. (justia.com)
  • In another embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is less than 25 amino acids in length. (justia.com)
  • In a more preferred embodiment, the peptide comprising the sequence of SEQ ID NO. 4 is 18 amino acids long. (justia.com)
  • DNA sequences that form the coding region for retroviral enzymes including reverse transcriptase, protease, and endonuclease/integrase. (lookformedical.com)
  • A4 is often an aspartic acid (Asp), indicating that a protease which cleaves between the methionine (Met) at position 596 (Met.sub.596 using the numbering system according to Kang J. et al. (everypatent.com)
  • Kunitz-like domain"--A protease inhibitor similar to soybean trypsin inhibitor or a nucleic acid sequence encoding a protease inhibitor which is similar to the soybean trypsin inhibitor. (everypatent.com)
  • Based on the 18S rRNA sequence, the shortest hereditary distance was found between A. agkistrodontis and Raillietiella spp. (unl.edu)
  • To test this hypothesis, the phylogenetic composition of faecal microbiomes was investigated by a 16S rRNA gene amplicon sequence-based approach for Bacteria and Archaea . (springer.com)
  • This new DNA-binding motif shares structuralhomology with T4 RNase H, E. coli endonuclease III and Bacillus subtilisDNA polymerase I. The structural homology suggests a mechanism forsubstrate recognition by these enzymes. (embl.de)
  • The iPSCs were transduced with CRISPR guide RNAs, Cas9 endonuclease and a donor homology template. (nature.com)
  • We found that the majority of predicted ARDs (pdARDs) were distantly related to known ARDs (mean amino acid identity 29.8%) and found little evidence supporting their transfer between species. (nature.com)
  • Multiple MTG8-related sequences are found in several vertebrate species, from fish to mammals, albeit not in a urodele. (embl-heidelberg.de)
  • Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
  • A novel DNA-binding motif shares structural homology to DNA replicationand repair nucleases and polymerases. (embl.de)
  • In preferred embodiments, the present invention provides a nucleic acid encoding a neurogenesis inducing gene (e.g., early neurogenesis inducing gene). (justia.com)
  • DNA microarrays take advantage of the major feature of the DNA double helix, the sequence complementarity of the two-paired strands, by using DNA capture probes which are the complement of the expressed target sequence (mRNA, cRNA or cDNA made from the mRNA). (ddw-online.com)
  • Northern blot analysis and S1 protection and mapping experiments have revealed that the mRNA for MLCK is 5.5 kilobases in length, but there also exists a second mRNA of 2.7 kilobases that shares a high degree of homology with about 520 base pairs at the 3' end of the cDNA for MLCK. (torvergata.it)
  • Yet, this assumption is poorly supported by empirical evidence due to the distant homologies between known ARDs (mostly from culturable bacteria) and ARDs from the intestinal microbiota. (nature.com)
  • In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)
  • The qPCR cycle quotient (Cq) value representative of MADC2 detection in 3,156 males, the detection of tissue control cannabinoid synthesis for 8,200 samples, and the absence of MADC2 detection in 5,047 non-males demonstrate a robust high-throughput real-time genotyping assay for Cannabis . (cannaqa.wiki)
  • Therefore, much larger numbers of sequences may be required for reliable detection of mutations than for most other applications of shotgun metagenomics. (pensoft.net)
  • Interestingly, the composition of membrane-associated lipoteichoic acid was not affected. (asm.org)
  • The TAF homology (TAFH) or Nervy homology region 1 (NHR1) domain is a domain of 95-100 amino acids present in eukaryotic proteins of the MTG/ETO family and whereof the core ~75-80 residues occur in TAF proteins. (embl-heidelberg.de)
  • Despite the gene's repetitive and GC-rich sequences, 13% of RPGR gene copies showed mutation correction and conversion to the wild-type allele. (nature.com)
  • CRISPR gRNAs were selected to minimize the off-target profile and to maximize the degree of homology with a target sequence directly centered on the mutation site. (nature.com)
  • Also known as nervy homology region 1 (NHR1). (embl-heidelberg.de)
  • Systematic deletion mutagenesis of Tsp localized the binding site to amino acids 206-307, a region that completely encompasses the putative PDZ domain (217-301). (embl.de)
  • We are investigating how these steps are controlled by the sequence of the promoter and by accessory proteins to obtain a quantitative understanding of gene expression. (rutgers.edu)
  • Such 'high-level' analyses are computationally expensive and often require parallel computing on distributed computing systems. (biomedcentral.com)
  • Oxr1p belongs to the group of TLDc domain containing proteins that are highly conserved in higher eukaryotes. (upstate.edu)
  • Early studies of DNA melting and reformation were carried out in aqueous solutions and yielded important information about the dependence of melting temperature (Tm) on the G+C composition and salt concentration, as well as information on the dependence of the rate of reassociation on the sequence complexity of the nucleic acid. (ddw-online.com)
  • Graphs have proven to be a powerful tool for coping with the complexity of genome-scale sequence alignments. (biomedcentral.com)
  • Coconuts from Asia and the South Pacific have been found to have viroids with similar (homologous) nucleic acid sequences of CCCVd. (wikipedia.org)
  • PDZ domain proteins are frequently associated with the plasma membrane, a compartment where high concentrations of phosphatidylinositol 4,5-bisphosphate (PIP2) are found. (embl.de)
  • Within L. monocytogenes , sequences homologous to gtcA were found in all serogroup 4 isolates but not in strains of any other serotypes. (asm.org)
  • Studies on the GM process have shown that the foreign gene inserts invariably damages the genome, scrambling and rearranging DNA sequences, resulting in inappropriate gene expression that can trigger cancer [3, 4]. (i-sis.org.uk)
  • Although it is still not possible to isolate and culture the vast majority of microorganisms (estimated to be over 95%), analysis of total nucleic acid from microbiome samples has enabled significant advances in the field. (neb.com)
  • Comparison of the three predicted human MTG8-related polypeptides to their Drosophila counterpart (nervy) highlights four separate regions of sequence conservation that may correspond to distinct domains. (embl-heidelberg.de)
  • The genome atlases allowed for distinguishing distinct DNA structures and highlighted suspicious high transcription zones. (mdpi.com)
  • This study reveals a new mechanism for the fluid gain of beneficial mutations in genetic regions undergoing active recombination in viruses and illustrates the value of long read sequencing technologies for investigating complex genome dynamics in diverse biological systems. (elifesciences.org)
  • Further experiments will be required to uncover the physiological role of the interaction suof Oxr1 with the V-ATPase, and whether the observations obtained for the yeast system are conserved in higher organisms, including humans. (upstate.edu)
  • Although there are effective vaccines and treatment strategies against hepatitis B (HB), it is still a significant health concern worldwide that can present in acute, permanent, severe liver failure and cancer forms resulting in high morbidity and death. (sciendo.com)
  • An expressed sequence tag (EST) library was constructed from mRNA obtained from fat bodies of A. mylitta larvae that had been challenged by infection with Escherichia coli cells. (biomedcentral.com)
  • Our analysis generates both the first wheat leaf transcriptional map and one of the most comprehensive descriptions to date of the developmental history of chloroplasts in higher plants. (biomedcentral.com)
  • As for many other gram-positive bacteria, the cell wall of L. monocytogenes contains large amounts of the anionic polymer teichoic acid (TA), covalently linked to peptidoglycan. (asm.org)