• This was also seen when preactivated platelets, which cannot undergo further morphological changes, were used, and when platelets were activated in the presence of shape-change inhibitors such as cytochalasin D and vincristine. (nature.com)
  • Hg(II), cytochalasin B (cytB) and forskolin, inhibitors of glucose transportation via GLUTs, decreased glucose transportation but had small inhibition on CH3As(OH)2 transportation. (edrc2013.org)
  • Cytochalasins are fungal metabolites that have the ability to bind to actin filaments and block polymerization and the elongation of actin. (wikipedia.org)
  • The effects of many different cytochalasins on actin filaments were analyzed and higher concentrations (2-20 μM) of CD were found to be needed to remove stress fibers. (wikipedia.org)
  • How do actin filaments form higher-order assemblies that produce and respond to force? (mechanobio.info)
  • In addition, Atf3 mRNA levels are increased in response to cytochalasin D treatment, an inducer of chondrocyte maturation. (biomedcentral.com)
  • We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. (umassmed.edu)
  • Cells were washed and incubated for 20 hrs in culture medium with the cytokinesis blocker, Cytochalasin B. Cells were then washed, fixed, stained and scored for micronuclei (MN) in binucleated cells. (cdc.gov)
  • This test is based on the principle that cytochalasin D very effectively inhibits platelet function by inhibition of the contractile elements. (wikipedia.org)
  • Clots also had ~ 60% lower fibrinogen (fibrin-based EXTEM activated test with platelet inhibitor cytochalasin D A10) and 36%-50% reduced fibrinogen-to-platelet ratio (11%-14% vs. 22% baseline). (edu.au)
  • Cytochalasins are known to bind to the barbed, fast growing plus ends of microfilaments, which then blocks both the assembly and disassembly of individual actin monomers from the bound end. (wikipedia.org)
  • Actin microfilaments have been widely studied using cytochalasins. (wikipedia.org)
  • Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. (techscience.com)
  • For example, cytochalasin A and cytochalasin B can also inhibit the transport of monosaccharides across the cell membrane, cytochalasin H has been found to regulate plant growth, cytochalasin D inhibits protein synthesis and cytochalasin E prevents angiogenesis. (wikipedia.org)
  • An Enantioselective, Modular, and General Route to the Cytochalasins: Synthesis of L-696,474 and Cytochalasin B" (PDF). (wikipedia.org)
  • This result, together with observations of a relatively high cell stiffness probed through integrins compared with the small stiffness probed through E-cadherins, suggests that mechanical force transmission might also be important in regulating cell spreading. (illinois.edu)
  • This is accompanied by increased Atf3 promoter activity in cytochalasin D-treated chondrocytes. (biomedcentral.com)
  • High salt enhanced the activity of the p38 mitogen-activated protein kinase (p38/MAPK) and increased the interleukin (IL)-8 release in a p38/MAPK-dependent manner. (frontiersin.org)
  • Glut 4 complexed with cytochalasin B was retrieved from the protein data bank (Rcsb.pdb). (ijpsr.com)
  • The transient increase of Orai1 protein abundance was abrogated by Rac1 inhibitor NSC23766 (50 μM) and by prevention of actin reorganization with cytochalasin B (1 μM). (biomedcentral.com)
  • Our results indicate that the active factor is either a high molecular weight protein or protein complex and is not the respiratory protein hemocyanin. (nature.com)
  • These in-vitro investigations suggest that Quercetin, in particular, could be a strong therapeutic agent with greater therapeutic efficacy than Cytochalasin B in the therapy of cancer by inhibiting GLUT4. (ijpsr.com)
  • Visikol scientists used the cell painting technique to elucidate the effects of a small molecule anti-proliferative therapeutic and cytochalasin B in cancer cells. (visikol.com)
  • As a result of the inhibition of actin polymerization, cytochalasins can change cellular morphology, inhibit cellular processes such as cell division, and even cause cells to undergo apoptosis. (wikipedia.org)
  • Cytochalasins have the ability to permeate cell membranes, prevent cellular translocation and cause cells to enucleate. (wikipedia.org)
  • NIH 3T3 cells were treated with cytochalasin D (2 μM) for 120 min, or with cytochalasin following 30 min pretreatment with latrunculin B (5 μM). (biomedcentral.com)
  • Cells exert traction forces on the ECM and generate tension at focal adhesions through actin stress fibers , which are higher-order structures in the cytoplasm that consist of parallel contractile bundles of actin and myosin filaments. (mechanobio.info)
  • High-content screening differs from traditional drug discovery methods in that it provides richer datasets than traditional assays, allowing for the examination of the specific effect of compound treatments on specific sub-populations of cells, as well as providing access to more complex measurements than can be accomplished with traditional assay formats. (visikol.com)
  • Visikol's future perspectives include incorporating high-throughput robotics and automation to seed cells and deliver compounds to microplates, improving high throughput technology in imaging and its computational image processing pipeline by integrating the data generated with AI and machine learning tools. (visikol.com)
  • C3H10T1/2 cells were cultured as pellets in either 4µM cytochalasin D or 4µM nocodazole and subjected to 3 days of cyclic hydrostatic compression (1 Hz, 5 MPa, 2 h per day). (openorthopaedicsjournal.com)
  • A correct IZUMO1 repositioning following in vitro induction of F-actin depolymerization, by cytochalasin D treatment, occurred. (ijbs.com)
  • CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor. (techscience.com)
  • It was rapidly demonstrated that the toxins target the cytoskeleton and inhibit the actin polymerization by specifically sequestering the G-actin monomers with a high affinity [4] , unlike cytochalasin D that targets the actin filament [5] . (beilstein-journals.org)
  • High affinity monosaccharide (KM ≈ 20 µM):H+ symporter, Stp6 (takes up glucose, 3-O-methylglucose, mannose, fructose, galactose and to a lesser extent, xylose and ribulose. (lbl.gov)
  • This compound activated the tyrosine kinase domain of the IR β-subunit at concentrations of 1 μmol/l or less but had no effect on insulin binding to the IR α-subunit even at much higher concentrations. (diabetesjournals.org)
  • Higher concentrations led to inhomogeneous formulations. (europa.eu)
  • High salt caused reduced spontaneous movement, degranulation and impaired production of reactive oxygen species (ROS) while leaving neutrophil viability unchanged. (frontiersin.org)
  • Erythrocyte cellular and nuclear dimensions of tetraploid mud loach were significantly higher than those of diploids, as expected. (springeropen.com)
  • The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)
  • However, recent advances in high-content screening have made it possible to get a better understanding of how drugs impact cellular pathways, accelerating the drug discovery process. (visikol.com)
  • High-content screening is a type of drug discovery method that utilizes high-throughput imaging and analysis to assess multiple endpoints simultaneously at a cellular resolution. (visikol.com)
  • Instead, the induction rate of tetraploidy was highest under 7500 psi hydrostatic pressure treatment. (springeropen.com)
  • The production rate was highest in the experimental groups treated with hydrostatic pressure alone, whereas the production rate was lowest in groups treated under hydrostatic pressure with chemical treatment. (springeropen.com)
  • Cytochalasin has found practical application in thromboelastometry (TEM) whole blood assays for the assessment of fibrinogen and fibrin polymerization disorders in the FIBTEM assay on ROTEM. (wikipedia.org)
  • Cell Painting is a high-content, multiplexed image-based assay used for cytological profiling. (visikol.com)
  • High-content screening is a powerful tool for drug discovery, and Visikol's Cell Painting Assay is a game-changer in this field. (visikol.com)
  • The human lymphocytes micronucleus assay has been extensively used in Europe since the cytochalasin-B method was introduced. (uab.cat)
  • The outcome of the study with 30 European laboratories surveyed, reflects an immature state of the micronuclei assay in Europe, with (a) disparity in criteria and methodologies, (b) high diversity in the methods of statistical analyses employed, (c) non-uniform criteria for determining positive responses, and (d) a still rare use of new or emerging methodologies. (uab.cat)
  • It cannot be overstated that the rate at which cancer uses glucose for proliferation is one of the many variables contributing to the alarmingly high mortality rate of cancer over time. (ijpsr.com)
  • G-actin regulated genes were induced by treatment with cytochalasin D ( CD , 2 μM, 90 min) and repressed by latrunculin B ( LB , 5 μM). (biomedcentral.com)
  • The compounds were characterized using infrared, nuclear magnetic resonance, and high-resolution mass spectrometry techniques. (bvsalud.org)
  • The results of this in-silico study showed that the docking scores of the 10 compounds were higher than those of the co-crystallized compound. (ijpsr.com)
  • Cytochalasins can also have an effect on other aspects of biological processes unrelated to actin polymerization. (wikipedia.org)
  • A decrease in the nuclear division index denoting loss of viability was noted at the highest dose (10 ug/ml). (cdc.gov)
  • Stimulation of neutrophils with phorbol-12-myristate-13-acetate (PMA) overcame high salt-induced impairment in ROS production and restored antimicrobial activity of neutrophils. (frontiersin.org)
  • Effects of Cytochalasin and Phalloidin on Actin" (PDF). (wikipedia.org)
  • high-energy' such possibilities' were moreefficient, excellent kingdom fries owned to existing and first-year experiment. (postgrp.com)
  • The highest concentration tested was 5000 µg/mL, tested as supplied (equivalent to 4585 ug enzyme concentrated dry matter/mL). (europa.eu)
  • Studies done with cytochalasin D (CD) have found that CD-actin dimers contain ATP-bound actin upon formation. (wikipedia.org)
  • When plasma from hyperlipidaemic patients with or without coronary heart disease and from age‐matched healthy controls was studied, the plasma oxidizability was found to be highest in the patients with coronary heart disease and lowest in the controls. (iospress.com)
  • Correction was associated with lower plasma tissue factor, higher plasminogen activator inhibitor-1, and lower D-dimers (5% of controls at 60 min). (edu.au)
  • Strand specific total-RNA-seq following DSN normalisation and validation by qRT-PCR from NIH3T3 stimulated by serum or Cytochalasin D in presence or absence of LatrunculinB and/or U0126 drugs. (nih.gov)
  • The control revertant numbers for 2 of the strains (TA1535 and TA1537) are unusually high, and therefore the reliability of the study is questionable. (europa.eu)
  • This guarantees lowest latencies and highest reliability of the system. (judson.pl)
  • Whereas inhibition of p38/MAPK did not result in improved neutrophil defense, pharmacological blockade of the phagocyte oxidase (PHOX) or its genetic ablation mimicked the impaired antimicrobial activity detected under high salt conditions. (frontiersin.org)
  • This result is comparable to Cytochalasin H another non-coxsackie-adenovirus receptor binding Ad (Ad37p), where substitution of one amino acid abolishes virus-cell connection. (grandlacs-med-journal.com)
  • Here we show that, in neutrophils, increases in Na + (high salt, HS) impair the ability of human and murine neutrophils to eliminate Escherichia coli and Staphylococcus aureus . (frontiersin.org)
  • Currently, there is no human vaccine, and the available treatments are associated with toxicity, high cost, and the emergence of resistant strains. (bvsalud.org)
  • In our work, we characterized subcellular organization of F-actin in human high- and low-quality spermatozoa (A- and B-SPZ), respectively, showing that F-actin over-expression in sperm head of B-SPZ affected IZUMO1 localization. (ijbs.com)
  • Interestingly, F-actin depolymerization was also associated with a correct acrosome repositioning, thus to favor a proper acrosome reaction onset, with changes in sperm nuclear size parameters and histone acetylation rate reaching high-quality conditions. (ijbs.com)
  • Due to their chemical nature, cytochalasins can help researchers understand the importance of actin in various biological processes. (wikipedia.org)
  • Cytochalasin B is a chemical that disrupts microfilament formation. (quizlet.com)
  • Early menstruation, late menopause, puberty-related obesity, high estrogen levels in postmenopausal women and not breastfeeding are all examples of hormonal imbalances that increase women's chances of developing cancer 8, 9, 10, 11, 12, 13 . (ijpsr.com)
  • Hence, we conclude that high salt-impaired PHOX activity results in diminished antimicrobial activity. (frontiersin.org)
  • In conjunction with earlier results, our findings Cytochalasin H also strongly suggest that sB2AR is equivalent to CD46. (grandlacs-med-journal.com)
  • Development in higher order organisms commences at conception and continues into old age. (mechanobio.info)
  • The production rate was 3.1 %, which was highest in all experimental groups exposed to 6000 psi for 10 min after being fertilized for 100 min. (springeropen.com)
  • The following figure explains a signal, if sampled at a higher rate than 2w in the frequency domain. (judson.pl)
  • High salt diets ( 1 , 2 ), renal impairment ( 3 - 6 ), inflammation, and infection ( 7 - 11 ) can induce Na + accumulation in skin tissues that can be simulated by addition of approximately 40 mM NaCl to standard cell culture media (= high salt condition, HS) ( 2 , 8 , 12 , 13 ). (frontiersin.org)
  • genome-edited to contain tumor-associated genetic driver mutations revealed by The Malignancy Genome Atlas project for glioblastoma (GBM) results in formation of high-grade gliomas. (grandlacs-med-journal.com)
  • Rat GLUT1 was selected for this research because it includes a higher level of CH3As(OH)2 transportation than individual GLUT1 SMAD9 9. (edrc2013.org)
  • Thermal undetected cheats Power TDP call of duty modern warfare 2 buy hacks the average power, in watts, the processor dissipates when operating at Base Frequency with all cores active under an Intel-defined, high-complexity workload. (judson.pl)