High affinity functional protein. Medical search. Frequent questions

Monoclonal mouse Strep -tag antibodies are used to detect recombinant proteins carrying the short Strep -tag affinity tag epitope with high specificity and sensitivity. (qiagen.com)
Proteins can be detected with high specificity and sensitivity using mouse monoclonal Strep -tag or Anti·His antibodies. (qiagen.com)
This project aims to develop a revolutionary screening platform that will allow for the rapid isolation of hundreds of high affinity and specificity synthetic ligands for proteins in a highly parallel fashion. (nih.gov)
2009 identified 21 families of these substrate capture proteins, each with a different specificity predicted by genome context analyses. (tcdb.org)
These ligands, having multiple binding portions, may be expected to bind with high affinity and specificity. (elsevierpure.com)
Ablexis' AlivaMab® Mouse platform is used by the majority of the world's leading pharmaceutical companies as the platform of choice for the rapid and efficient generation of diverse panels of antibodies with inherent qualities needed for antibody drugs, including affinity, potency, specificity and developability. (pharmiweb.com)
In particular their high stability should allow for simple conjugation to radioisotopes and their small size and high affinity binding with precise specificity should allow for superior targeting of tumors. (bigcountryhomepage.com)
The kinetic parameters and the pharmacological specificity of a high affinity leukotriene D4 (LTD4) receptor antagonist, ICI-198615, binding to guinea pig lung membranes were characterized. (aspetjournals.org)
However, the molecular mechanisms underlying this seemingly relaxed sequence specificity are not understood and functional studies have focused on 3΄ intronic sites only. (crick.ac.uk)
We show that a two-domain RRM1-ZnF unit recognizes a GGA-centered motif enriched in RBM10 exonic sites with high affinity and specificity and test that the interaction with these exonic sequences promotes exon skipping. (crick.ac.uk)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)

The binding of allosteric modulators to a target protein induces a conformational change in the protein structure and changes the activity of orthosteric ligands ( Figure 1 ). (frontiersin.org)
The researchers discovered that the chelating systems featuring three and four hydroxypyridinone functional groups show a high affinity for lanthanide metals, and in particular europium, suggesting that these peptoid-based chelators could be used to design ligands tailor-made for a wide range of applications with f-block metals, such as chemical separation processes, optical device optimization, and pharmaceutical development. (lbl.gov)
The magnetic beads with surface-reactive amino groups allow immobilization of ligands such as proteins, peptides, carbohydrates or other target specific molecules. (gbiosciences.com)
Finally, amine reactive heterobifunctional cross-linkers can be used to introduce other functional groups for coupling ligands. (gbiosciences.com)
Here, we describe an approach called ''consolidated" ligands to probe structural and functional activities of multidomain proteins. (elsevierpure.com)
The ligands with two binding portions can block simultaneously the one SH2 and one SH3, or two SH3 domains of Grb'2 with better than 100 nM affinity. (elsevierpure.com)
This study extends the feasibility of consolidated ligands, and this general protocol can be used to identify the interfaces between domains for rational design of higher affinity reagents. (elsevierpure.com)
The particles are used to specifically conjugate primary amine-containing targeted protein or ligands in a simple mix-and-go format for use in custom affinity purification experiments with significant low non-specific binding. (nanowerk.com)
A recently developed algorithm allows Rigid Body Docking of ligands to proteins, regardless of the accessibility and location of the binding site. (kobv.de)
In drug discovery, it is of utmost importance to accurately calculate the free energies of binding ligands to various protein targets, such as enzymes and receptors. (lu.se)

The following product was used in this experiment: FceR1 alpha Monoclonal Antibody (MAR-1), Functional Grade, eBioscience™ from Thermo Fisher Scientific, catalog # 16-5898-82, RRID AB_469137. (thermofisher.com)
The two-step affinity purification system, using the Strep- tag Antibody for detection, is highly suited for applications where high purity is at a premium or is difficult to achieve. (qiagen.com)
In this study, the sp2 gene was cloned and expressed in Escherichia coli to produce a 6xHis-tagged fusion protein, which was then used to generate a polyclonal antibody. (hindawi.com)
Antiverse has successfully identified functional antibody clusters targeting two G-protein-coupled receptors (GPCRs), both displaying versatile binding profiles and nanomolar affinity. (ddw-online.com)
This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. (abcam.com)
The antibody also binds to P462L-substituted S318-510 fragments of the SARS spike protein. (abcam.com)
Precisely, this antibody binds to the 'open' conformation of the spike protein to the amino acids 318-510 in the S1 domain of the SARS-CoV as well as SARS-CoV-2 strains (32245784, Joyce et al. (abcam.com)
DARPins combine the small molecule feature of rapid tumor penetration and clearance from the body, with the antibody-like ability of binding to a wider range of proteins and other cell surface targets. (bigcountryhomepage.com)
Linking these isolated, shortened peptides (PWSLNR and SGVYK) with an antibody-binding peptide (NKFRGKYK) created dual-functional peptides that are capable of QD surface functionalisation by antibodies. (whiterose.ac.uk)
We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)
To ensure that recently developed antibody tests for seroepidemiology of Chlamydia trachomatis (Ct), the causative agent of urogenital chlamydia and the blinding eye disease trachoma, have high performance, we generated a chimeric antibody to the immunodominant Ct antigen Pgp3. (cdc.gov)

The interaction of sGAG with α2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and serine protease HTRA1 (HTRA1) was confirmed in reverse assays. (degruyter.com)
FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. (thermofisher.com)
This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. (nih.gov)
The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). (nih.gov)
This protein has also been found to be a functional receptor for Coxsackievirus A10 and may be an alternative entry receptor for SARS-CoV-2. (nih.gov)
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. (novusbio.com)
Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. (novusbio.com)
Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. (novusbio.com)
The low expression level of the angiotensin converting enzyme 2 receptor and lower receptor affinity to SARS-CoV-2 spike, which could be overcome by overexpression of canine angiotensin converting enzyme 2 in trans, strengthened the cellular barrier to productive infection. (cdc.gov)
The growth factor receptor-binding protein Grb2, composed of one SH2 and two SH3 domains, acts as an adaptor protein in transduction of signals from tyrosine-phosphorylaled proteins (EGF-receptor, or SHC), to the Ras dependent pathways. (elsevierpure.com)
Based on multiplex screening assays performed on the Beacon® platform, these SARS-CoV-2 S1 antibodies may be binned into subsets based on their binding to the receptor binding domain (S1 B ) of SARS-CoV2, blocking of S1 B binding to the ACE2 receptor, and cross-reactivity with the S1 spike protein of the SARS-CoV virus. (pharmiweb.com)
Binding of radiolabeled high affinity antagonist to leukotriene D4 receptor in guinea pig lung membranes: interconversion of agonist-receptor binding affinity states. (aspetjournals.org)
Lymphocyte activation gene-3 (LAG-3) is a coinhibitory receptor associated with impaired T-cell function and is frequently coexpressed with programmed cell death protein-1 (PD-1) in the context of human cancers. (aacrjournals.org)
Proteomic analysis reveals that TRP channel-associated factor 2 (TCAF2), a partner protein of the transient receptor potential cation channel subfamily M member 8 (TRPM8), is overexpressed in TPCs from patients with CRCLM. (bvsalud.org)
The functional high affinity IL-12 receptor is composed of at least IL12rb1 and IL12rb2. (lu.se)
These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)

Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
The Two-Step Affinity Purification System, which is based on the proven 6xHis tag and the short Strep -tag II, enables simple and highly efficient purification of ultrapure proteins in a fast and standardized procedure. (qiagen.com)
Recombinant proteins carrying both tags are purified sequentially on Ni-NTA and Strep -Tactin matrices (see figure Ultrapure protein in two steps. Thioredoxin, expressed in NIH-3T3 cells, was purified using the Two-Step Affinity Purification System. [A] Coomassie-stained gel [B] Silver-stained gel. "> Ultrapure protein in two steps ). (qiagen.com)
98% pure) protein (see figure Two-step affinity purification procedure. Two-step affinity purification procedure. "> Two-step affinity purification procedure ). (qiagen.com)
The standardized purification procedure also increases throughput by eliminating the need for protein-specific purification protocol development and optimization. (qiagen.com)

When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. (thermofisher.com)
For example, alkaline vacuole/lysosome are deficient in autophagy, Golgi pH regulates its ability to glycosylate proteins and failure to maintain endosomal pH perturbs with its ability to recycle receptors to the Plasma membrane or, the trans-Golgi. (upstate.edu)
Therefore, Sig1R demonstrates properties that can be attributed to both chaperone proteins and receptors. (frontiersin.org)
Nicotinic acetylcholine receptors are transmembrane oligomeric proteins that mediate interconversions between open and closed channel states under the control of neurotransmitters. (unige.ch)
Coupling newly developed nanomaterials with other recognition elements such as nanobodies, peptide aptamers, and protein receptors would open up new avenues for electrochemical immunoassays and further extend their applications. (cdc.gov)

In the interaction with proteins, lead binds with virtually every available functional group, including sulfhydryl, amine, phosphate, and carboxyl groups, with sulfhydryl having the highest affinity. (cdc.gov)
Toluene binds to TodS with high affinity (Kd = 684 +/- 13 nM) and 1:1 stoichiometry. (nih.gov)
Binds to IL-12 with a low affinity. (lu.se)
Thyroid-binding globulin (TBG) is produced in the liver and is a circulating protein that reversibly binds thyroid hormones3,5,3'-triiodothyronine (T3) and thyroxine (T4) and carries them in the bloodstream. (medscape.com)

Sig1R is an endoplasmic reticulum membrane protein that, in addition to its promiscuous high-affinity ligand binding, has been shown to have chaperone activity. (frontiersin.org)
This proposal presents an innovative approach for screening and selecting a new class of highly stable protein capture reagents and developing a new versatile approach for ligand immobilization that together enable rapid production of cyclotide-based microarrays for proteomics research. (nih.gov)
A higher order consolidated ligand with three binding portions was developed and high affinity binding was achieved. (elsevierpure.com)
We mostly used molecular mechanics (MM) in order to model the protein-ligand interactions, which is more approximate than quantum-mechanical (QM) methods, but necessary to reduce the computational cost when doing calculations on protein-ligand systems, which often contain tens of thousand of atoms. (lu.se)
In one study of a large set of protein-ligand complexes, we tried to improve the free energies of binding by using MD simulations with QM-derived charges, which sometimes led to improved results, but not always. (lu.se)
This entropy largely stems from the fluctuation of the protein and ligand. (lu.se)
Additionally, we compared how MD and grand-canonical Monte Carlo (GCMC) can be used to assess dynamics and thermodynamics of protein-ligand binding for both buried and solvent-exposed binding sites. (lu.se)

2023 ). (1) the BioMNY complex with ATPase (BioM) and transmembrane protein (BioN). (tcdb.org)
Some transporters have a conserved transmembrane protein and two nucleotide binding proteins similar to those of ABC transporters. (tcdb.org)

This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. (abcam.com)
The antimicrobial functionality of LF is dependent on its protein conformation and milieu conditions (Naidu and Arnold, 1997). (ift.org)

Particularly, hyaluronic acid and its derivatives bind with high affinity to cell surface protein, CD44 enriched tumor cells. (intechopen.com)

The expressed protein in Escherichia coli was used to generate polyclonal antibodies. (hindawi.com)
The company has raised $3 million in funding, which will enable in-house development of the antibodies showing the highest affinity blocking function. (ddw-online.com)
Presence of both antibodies delivers a blocking action of the SARS-COV RBD-ACE2 interaction, by binding two distinct and functional epitopes (16796401). (abcam.com)
SAN DIEGO and EMERYVILLE, Calif., April 22, 2020 /PRNewswire/ -- Ablexis, LLC , AlivaMab Discovery Services, LLC ("ADS") and Berkeley Lights, Inc. today announced the rapid generation and recovery of sequences encoding a rich panel of human antibodies against the S1 spike protein of the SARS-CoV-2 virus. (pharmiweb.com)
Using the Berkeley Lights platform with our process of ultra high-throughput function-first screening, we efficiently recovered different categories of antibodies with diverse activities, each of which we believe could contribute to the fight against this terrible disease. (pharmiweb.com)
AlivaMab Discovery Services immunized AlivaMab Mouse by expressing native trimeric SARS-CoV-2 spike protein in vivo using its propriety AMMPD-DNA technology, a process validated for rapidly eliciting strong IgG titers, and molecularly diverse, high affinity (very low picomolar) antibodies. (pharmiweb.com)
135 antibodies against 65 mainly immunoregulatory proteins, we of a multitude of different genes (8, 9). (lu.se)

As opposed to single strand (ss) DNA binding proteins such as SSB and RPA, which protect and occlude ssDNA from recombination, RAD52/Redβ SSAPs promote recombination and share several biochemical similarities. (nature.com)
These shared biochemical, protein sequence and functional similarities suggest the existence of an ancestral annealing mechanism involved in HR. (nature.com)
Biochemical assays revealed that the BioM, N, and Y proteins form stable complexes in membranes of the heterologous host. (tcdb.org)

As evaluated by co-immunoprecipitation experiments, the dsDNA recombination function relates to the Redα-Redβ protein-protein interaction, which requires not only contacts in the C-terminal domain but also a region near the N-terminus. (nature.com)
Because the nucleoprotein filament formed with C-terminally truncated Redβ has altered properties, the second C-terminal function could be due to an interaction required for functional filaments. (nature.com)
Further experiments will be required to uncover the physiological role of the interaction suof Oxr1 with the V-ATPase, and whether the observations obtained for the yeast system are conserved in higher organisms, including humans. (upstate.edu)
After cell lysis and clearing of the lysate, proteins are initially purified using an immobilized-metal affinity chromatography procedure that is based on the proven 6xHis-tag-Ni-NTA interaction. (qiagen.com)
W278A/W279A/Y380A lost the ability to bind β-CD although a trace affinity was still observed, perhaps reflecting a weak interaction in the substrate binding cleft. (dtu.dk)
Protein-protein interaction (PPI) network analysis presents an essential role in understanding the functional relationship among proteins in a living biological system. (nyu.edu)
Our approach is tested with three protein-protein interaction networks and performs well with significant random noises of deletions and insertions in true PPIs. (nyu.edu)
The high-affinity interaction of LF with pore-forming outer-membrane proteins (OMPs) of Gram-negative enterics, including Escherichia coli , is critical for the antimicrobial outcome of LF (Gado et al. (ift.org)
The interaction of LF with microbial surfaces-OMPs of Gram-negative bacteria in particular-has led to other antimicrobial mechanisms, such as the inhibition of microbial attachment to sub-epithelial matrix proteins and detachment of bacteria from mucosal surfaces. (ift.org)

These age-related immunological changes resemble those seen during treated HIV-1 infection and include high levels of soluble inflammatory proteins, high levels of monocyte and T cell activation, T cell exhaustion and senescence, and low levels of naïve T cells ( 14 - 25 ). (frontiersin.org)
Gastrointestinal absorption of soluble lead salts in adult humans can be high during fasting (40-50%), but is about 3-15% when ingested with food. (cdc.gov)
Additionally, soluble IL-1R2 recruits soluble IL-1RAP with high affinity without impacting affinity for IL-1RA . (medchemexpress.com)

Botulinum neurotoxins (BoNTs) are bacterial proteins that cause the life-threatening disease botulism 1 . (cdc.gov)

Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
The main pathological features of AD are characterised by neurofibrillary tangles and senile plaques caused by the progressive deposition of Aβ peptides in the brain, composed mainly of 39-43 peptides generated by proteolytic cleavage of the Aβ precursor protein (APP). (bmj.com)
Among five QD-binding peptide candidates, a series of truncated peptides designed from two high-affinity peptides were subjected to an array-based binding assay with QDs to assess their functional core sequences and characteristics. (whiterose.ac.uk)

A combination of molecular simulation with hydrogendeuterium-exchange mass spectrometry and electron paramagnetic resonance spectroscopy reveal an asymmetry across the membrane: ATP-induced conformational changes in the cytosolic cavity promote unfolded pre-protein structure, while the exterior cavity favours its formation. (elifesciences.org)

When evidence suggests that homologous membrane transport proteins of the ABC2 type couple transport to ATP hydrolysis using a homologue of the ABC-type ATPases, we list these proteins in the ABC superfamily. (tcdb.org)

This ability to exploit structure within a pre-protein is an unexplored area of protein transport, which may apply to other protein transporters, such as those of the endoplasmic reticulum and mitochondria. (elifesciences.org)
The AAA-ATPase (ATPases associated with diverse cellular activities) valosin-containing protein (VCP), is essential for many cellular pathways including but not limited to endoplasmic reticulum-associated degradation (ERAD), DNA damage responses, and cell cycle regulation. (portlandpress.com)
They have been defined as monotropic integral membrane proteins located primarily in the endoplasmic reticulum (COX-1) and the perinuclear envelope (COX-2). (medscape.com)

However, unlike typical ABC transporters (E.I. Sun & M.H. Saier, unpublished results), they use small integral membrane proteins that are postulated to capture specific substrates. (tcdb.org)

BioMNY proteins are considered to constitute tripartite biotin transporters in prokaryotes. (tcdb.org)

Enzymatic cleavage assays represent an attractive approach where the functional active toxins are detected by monitoring the cleavage of their target substrates, or peptide mimics in most cases, by means of different techniques, such as immunological detection 18 , 19 , capillary electrophoresis 20 , fluorescence analysis 21 , Forster resonance energy transfer (ALISSA) 22 , and high performance liquid chromatography 23 . (cdc.gov)

High affinity mRNA in situ hybridisation and immunohistochemistry were complemented by functional genomic and bioimaging techniques. (uea.ac.uk)

Moreover, as the word 'coding' refers to the relationship between nucleic acids and proteins, rather than the mere transcription of DNA into RNA, it is logical to call the strand with the mRNA sequence the coding strand, as in the first example. (genomicglossaries.com)
The transcription of COX-1 yields a 2.7-kilobase (kb) messenger ribonucleic acid (mRNA) that encodes a 576-residue, 65-kd protein. (medscape.com)
Conversely, the transcription of COX-2 yields a 4.5-kb mRNA that encodes a 70-kd protein with roughly 70-75% homology to the COX-1 protein. (medscape.com)
Other studies show that the protein can attach to specific regions of messenger RNA (mRNA), which is a chemical cousin of DNA that serves as the genetic blueprint for protein production. (medlineplus.gov)
It is thought that the AFF2 protein helps control the process by which the mRNA blueprint is cut and rearranged to produce different versions of proteins (alternative splicing). (medlineplus.gov)

Transport of proteins across membranes is a fundamental process, achieved in every cell by the 'Sec' translocon. (elifesciences.org)
The encapsulation and compartmentalisation of cells has necessitated the evolution of machineries that conduct proteins across membranes, including for protein secretion and organellar import. (elifesciences.org)

Allosteric regulation is the regulation of protein activity by binding an effector molecule at a site other than the orthosteric or active site of a protein ( Figure 1 ). (frontiersin.org)
The substrate binding cleft of AMY1 is composed of two aglycone and seven glycone binding subsites, of which subsite -6 (key residue: Y105) is characterized by having the highest binding affinity and being situated at the opposite end of the enzyme molecule of SBS2 at a distance of 60 Å. (dtu.dk)
Protein separation conditions always pose the risk of denaturation or structural alteration of the LF molecule. (ift.org)
1994). The highly cationic N-terminus region of LF could facilitate charge-induced protein aggregation and inactivate the molecule. (ift.org)

The specific binding of [3H] ICI-198615 was dependent upon the concentration of membrane protein. (aspetjournals.org)
The Fe(III) complexes investigated had long enough lifetime to participate in electron transfer with other molecules in solution, if the concentration was high. (lu.se)
Abnormal serum thyroid hormones concentration with healthy functional gland: a review on the metabolic role of thyroid hormones transporter proteins. (medscape.com)

A functional model is presented in which IHF favors the contact between the TodT activator, bound further upstream, and the alpha-subunit of RNA polymerase bound to the downstream promoter element. (nih.gov)

VCP primarily identifies ubiquitylated proteins in these pathways and mediates their unfolding and degradation by the 26S proteasome. (portlandpress.com)
Valosin-containing protein (VCP, also p97, or Cdc48p in yeast) is an evolutionarily conserved, homo-hexameric, ubiquitin-selective, AAA-ATPase that functions in numerous ubiquitin-dependent protein quality control pathways. (portlandpress.com)
Objective: To investigate the regulation of stem cell-driven tissue renewal by canonical Wnt and TGFβ/bone morphogenetic protein (BMP) pathways in the native human colonic epithelium. (uea.ac.uk)
Furthermore, at very high concentrations of the photosensitiser a light-induced charge-disproportionation reaction outcompeted all other deactivation pathways. (lu.se)

2009 have a dedicated energizing module, but in 459 cases distributed among almost 100 gram-positive bacteria, different and unrelated substrate capture proteins share the same energy-coupling module. (tcdb.org)

Small angle neutron scattering (SANS) provides unique insight into biomacromolecular complexes by combining solvent contrast variation (H2O:D2O exchange) with either natural contrast between different classes of biomolecules (proteins, RNA/DNA, lipids/detergents) and/or by applying artificial contrast, i.e. deuteration of specific biomolecules. (lu.se)
In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)

High-throughput assays are indispensable for comprehensive functional proteome research. (nih.gov)

By mechanism, DNA annealing proteins are divided into ATPases capable of strand invasion (RecA, RAD51) and single strand annealing proteins (SSAPs) that do not utilize ATP 4 . (nature.com)

1991), none of the the structural details of the heterogeneous protein surface and experimental studies mentioned so far could provide un- takes into account a variety of short-range interactions, direct ambiguous information about oligomer structure. (lu.se)

In this respect, in contrast to predictors based for each serum protein was determined by comparing the samples upon tumor characteristics at the time of surgery, serum is a par- collected at the primary operation and then 3-6 mo later. (lu.se)
The results from this prevali- the field of cancer biomarkers (11-13), and that approach was dation study showed that patients could be classified into high- adopted here to define predictive serum biomarkers associated versus low-risk groups for developing metastatic breast cancer with tumor relapse in breast cancer patients. (lu.se)
This risk assessment was not dependent on the type of adju- decoding patterns of immunoregulatory serum proteins could re- vant therapy received by the patients. (lu.se)
Conse- demonstrated that this protein signature provided an added value quently, using minute amounts of nonfractionated serum (14) and compared with conventional clinical parameters. (lu.se)
TBG transports nearly 75% of serum T4 and T3 and has a 10-fold greater affinity for T4 than for T3. (medscape.com)

Single strand annealing proteins (SSAPs) like Redβ initiate homologous recombination by annealing complementary DNA strands. (nature.com)
Also, they share a similar protein architecture based on an N-terminal ssDNA binding domain of ~180 amino acids and a C-terminal extension that in the case of RAD52 is required for homologous recombination (HR) through specific protein-protein interactions 6 . (nature.com)

VCP identifies ubiquitylated substrates through numerous dedicated adaptor proteins and unfolds substrates by threading them through a central pore in the hexamer ( Figure 1 ). (portlandpress.com)
VCP interacts with adaptor proteins to identify ubiquitylated substrates for degradation by the proteasome. (portlandpress.com)

The reagents must be stable to thermal and proteolytic degradation, have high affinity, be easy to produce and present low cross-reactivity. (nih.gov)

Mutations in three genes coding for the amyloid precursor protein ( APP ), presenilin 1 ( PS-1 ), and presenilin 2 ( PS-2 ) account for most cases of early onset, autosomal dominant familial AD (FAD), but only for 2% of all the AD cases. (bmj.com)

As the assembly was carried out in the absence of ATP, this suggests that formation of the holoenzyme from the individual subcomplexes is solely driven by protein-protein interactions at the interface. (upstate.edu)
Unlike reversible disassembly, which requires ATP hydrolysis on V 1 to break protein-protein interactions, Oxr1p mediated disassembly of V-ATPase is ATP independent and therefore a novel mode of activity regulation. (upstate.edu)
Initially, a combination of geometrical and force-field based methods is used as a Coarse Docking strategy, considering only Lennard-Jones interactions between the target and pharmaceutically relevant atoms or functional groups. (kobv.de)

Recombinant proteins that carry two small affinity tags (the 6xHis tag and Strep -tag II) are efficiently expressed in E. coli , insect, or mammalian cells using pQE-TriSystem His· Strep vectors. (qiagen.com)
After elution from the Ni-NTA matrix using imidazole, recombinant proteins (which also carry the Strep -tag II epitope) are loaded directly onto a Strep -Tactin matrix. (qiagen.com)
IL-1R2 Protein, Human (HEK293) is a recombinant human IL-1R2 protein and is expressed in HEK293 cells. (medchemexpress.com)

The method adds a large amount of genetic information as a basis for complete analysis of TRPV1 ion channel genetics and its functional consequences. (researchgate.net)
Rarely, small deletions of genetic material from the AFF2 gene are associated with fragile XE syndrome, although how these deletions affect the protein and lead to intellectual disability is unknown. (medlineplus.gov)

These mechanisms include the ability of lead to inhibit or mimic the action of calcium and to interact with proteins. (cdc.gov)
1997) at higher how proteins interact with themselves in solution. (lu.se)

Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
The identification of the intracellular sGAG-interacting proteins could help to unravel these functions. (degruyter.com)

We also visualized several lipids that are bound by the protein and modeled how the human channel interacts with icilin. (bvsalud.org)

Glycosaminoglycans (GAGs) are multifunctional polysaccharides of the extracellular matrix (ECM) responsible for ECM hydration and binding of cations and proteins due to their negative charge. (degruyter.com)

By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
Decomposition of the high dimensional conformational space of bio-molecules into metastable subsets is used for data reduction of long molecular trajectories in order to facilitate chemical analysis and to improve convergence of simulations within these subsets. (kobv.de)
This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
Melko M, Douguet D, Bensaid M, Zongaro S, Verheggen C, Gecz J, Bardoni B. Functional characterization of the AFF (AF4/FMR2) family of RNA-binding proteins: insights into the molecular pathology of FRAXE intellectual disability. (medlineplus.gov)
How- recurrence after primary operation could be assessed for each pa- ever, recent developments in affinity proteomics have advanced tient, using her molecular portraits. (lu.se)

The National Cancer Institute's 'Therapeutically Applicable Research to Generate Effective Treatments' (TARGET) initiative seeks to identify therapeutic targets for high-risk paediatric tumours through comprehensive characterization and integrative analyses ( http://ocg.cancer.gov/programs/target ). (nature.com)
Kinetic analyses identified BioY as a high-capacity transporter which was converted into a high-affinity system in the presence of BioMN. (tcdb.org)

showing the cavities through the channel, with the protein in grey surface, the pre-protein pore constrictions in red (SecY) or purple (SecA) mesh, and the SecY plug in red helix. (elifesciences.org)

Oligonucleotide which displays specific binding to a protein or other target, often selected by an iterative cycle of affinity- based enrichment. (genomicglossaries.com)

Possibly, these two transmembrane proteins comprise the pathway for transmembrane transport. (tcdb.org)
The vast majority of proteins entering the secretory pathway are synthesized on ribosomes docked at ER translocons and are co-transationally translocated into the ER lumen. (stanford.edu)
Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)

BioMNY-mediated biotin uptake was severely impaired by replacement of the Walker A lysine residue of BioM, demonstrating dependency of high-affinity transport on a functional ATPase. (tcdb.org)
Proteins synthesized at the ER are subject to covalent modifications that include N- and O-glycosylation, disulfide bond formation, and in some cases, proline and lysine hydroxylation. (stanford.edu)

Moreover, a D614G mutation in the spike protein did not appear to affect SARS-CoV-2 cell tropism. (cdc.gov)

IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. (novusbio.com)
This 58-residue (6.5 kDa) basic (pI ¼ 10.5) isoelectric point of BPTI, with phosphate as the salting-out protein has long been thought to undergo self-association. (lu.se)

Hillman MA, Gecz J. Fragile XE-associated familial mental retardation protein 2 (FMR2) acts as a potent transcription activator. (medlineplus.gov)

The gastric iodide pump is, relationship between increasing incidence however, phylogenetically more primitive of goitre due to iodine deficiency and inci- than that of the thyroid, has a lower affinity dence of gastric cancer in some regions of for iodide and does not respond to thyroid the country [ 2 ]. (who.int)
TBG, transthyretin (TTR or prealbumin), and albumin are the 3 proteins that carry thyroid hormone in the body. (medscape.com)
In cases of high TBG, more thyroid hormone will be bound, decreasing free hormone in the blood. (medscape.com)
The total thyroid hormone level will be high, free thyroid hormone levels will be normal and TBG will be high. (medscape.com)
If a patient is truly hypothyroid or hyperthyroid, then TBG levels will be normal and the total thyroid hormone levels will be low or high, respectively. (medscape.com)

The cause of the accentuated and/or accelerated aging in people with HIV remains to be fully elucidated, with factors like HIV associated chronic immune activation and dysfunction, ART toxicity and the higher prevalence of traditional risk factors each having been implicated to contribute to age-associated comorbidity development ( 8 , 9 ). (frontiersin.org)
The model predicts that (in order to overcome the intrinsic stability of the B state and to produce the appropriate cooperativity) channel activation is driven by an A state with a Kd in the 50 nM range, hence some 140-fold stronger than the apparent affinity of the open state deduced previously. (unige.ch)
POINT's active clinical trials include FRONTIER, a phase 1 trial for PNT2004, a pan-cancer program targeting fibroblast activation protein-α (FAP-α), and SPLASH, the phase 3 trial for PNT2002 for people with metastatic castration resistant prostate cancer (mCRPC) after second-line hormonal treatment. (mywabashvalley.com)

To accelerate research in this field, new protein capture tools for the detection and identification of specific proteins are needed. (nih.gov)
In addition, the effectiveness of antitoxin treatment may vary depending on circulating toxin levels, so that rapid identification and quantification of BoNT proteins in clinical specimens and food is desirable for optimization of treatment. (cdc.gov)

Patients with MLLT1 -mutant tumours present at a younger age and have a high prevalence of precursor intralobar nephrogenic rests. (nature.com)

Protein crowding induces membrane curvatures through an entropic mechanism. (portlandpress.com)

The cryo-EM analysis also revealed a population of free V 1 bound to Oxidation Resistance 1 (Oxr1p), a poorly characterized protein that has been implicated in protecting cells from oxidative stress. (upstate.edu)
Most of the circulating testosterone is bound to carrier proteins (SHBG = sex hormone-binding globulin). (cdc.gov)

Crowding of asymmetric proteins results in an asymmetric lateral pressure across the membrane which can be used by cells in a number of biological processes involving membrane remodeling. (portlandpress.com)

Storage protein 2 (SP2) not only is an important source of energy for the growth and development of silkworm but also has inhibitory effects on cell apoptosis. (hindawi.com)
A recent study showed that another protein in silkworm hemolymph, storage protein 2 (SP2), can also inhibit staurosporine-induced HeLa cell apoptosis and ROS generation [ 8 ]. (hindawi.com)
Using 3′-UTR sequence probes, we have observed specific DNA protein binding with nuclear proteins from lymphocyte, astrocytoma, and neuroblastoma cell lines, but not from the microglia cell line. (bmj.com)
Next, Berkeley Lights, a leader in single cell selection and functional screening, deployed its platform capabilities using its Beacon® system to interrogate single plasma B cells over a two-day period. (pharmiweb.com)
The ER is the 'port of entry' for proteins destined for the cell surface and beyond. (stanford.edu)
We propose that the BPTI decamer, with a heparin chain threading the decamer channel, plays a functional role in the mast cell. (lu.se)

In competition studies, LTD4, stereoisomer (5R,6S)-LTD4, and leukotriene E4 (LTE4) competed with [3H]ICI-198615 binding to specific sites, with a stereoselectivity and a rank order of potency equivalent to those described in [3H]LTD4 binding studies and in functional studies. (aspetjournals.org)
SK&F 104353 and SK&F 104373, also competed with the [3H]ICI-198615 specific binding, with binding affinities comparable to those expected from the functional studies. (aspetjournals.org)
The TodS and TodT proteins form a previously unrecognized and highly specific two-component regulatory system in which the TodS sensor protein contains two input domains, each of which are coupled to a histidine kinase domain. (nih.gov)

Lead interferes with the synthesis of heme, resulting in accumulation of ALA in tissues and elevated excretion of ALA in urine, elevation of zinc protoporphyrin in erythrocyte, reductions in blood hemoglobin, and in a hypochromic, normocytic anemia at higher levels of exposure. (cdc.gov)

Anatomy15

Organisms13

Chemicals and Drugs95

Analytical, Diagnostic and Therapeutic Techniques and Equipment49

Phenomena and Processes83

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science7

Health Care1

Specificity11

Ligands10

Antibody11

Receptor16

Purification5

Receptors5

Binds4

Ligand7

Transmembrane protein2

Conformation3

Bind with high affinity1

Antibodies7

Biochemical3

Interaction9

Soluble3

Bacterial Proteins1

Peptides3

Mass spectrometry1

Membrane transpor1

Endoplasmic reticulum3

Integral membrane1

Transporters1

Chromatography1

Genomic1

MRNA5

Membranes2

Molecule4

Concentration3

Subunit1

Pathways4

Substrate1

Complexes2

Assays1

ATPases1

Structural1

Serum5

Homologous2

Adaptor2

Reagents1

Genes1

Interactions3

Recombinant3

Genetic2

Interact2

Intracellular2

Lipids1

Cations1

Molecular5

Analyses2

MeSH1

Enrichment1

Pathway3

Lysine2

Spike protein1

Residue2

Transcription1

Thyroid5

Activation3

Identification2

Prevalence1

Induces1

Bound2

Asymmetric1

Cell6

Specific3

Synthesis1