Heterotrimeric gtp binding proteins. Medical search. Frequent questions

Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. (wikipedia.org)
Muscarinic acetylcholine receptors are G protein-coupled receptors that respond to acetylcholine and play important signaling roles in the nervous system. (rcsb.org)
Only two PI3Ks are directly regulated downstream from G protein-coupled receptors (GPCRs): the class I enzymes PI3Kβ and PI3Kγ. (elsevierpure.com)
extrinsic plasma membrane proteins (cytoplasmic face) that function as a complex to transduce signals from G-protein coupled receptors to an effector protein. (umassmed.edu)
Heterotrimeric G proteins, comprising α, β and γ subunits, respond to extracellular signals generated by activated seven-transmembrane (7TM) receptors by modulating intracellular effector proteins such as enzymes and ion channels. (sigmaaldrich.com)
Identification from the molecular mechanisms that determine specificity of coupling interactions between gastrin-releasing peptide receptors (GRPrs) and their cognate heterotrimeric GTP-binding proteins is a fundamental step in understanding the signal transduction cascade initiated by receptor-ligand interaction. (biopaqc.com)
The EC50 for GRP was 3.5 nM which correlates well with the reported (1994) 46 235 The apparent receptor reconstitution method will allow molecular characterization of G protein coupling to other heptahelical receptors. (biopaqc.com)
Multiple endocytic pathways of G protein-coupled receptors delineated by GIT1 sensitivity. (duke.edu)
Here, we report that GIT1 overexpression regulates internalization of numerous, but not all, G protein-coupled receptors. (duke.edu)
GIT1 only affects the function of G protein-coupled receptors that are internalized through the clathrin-coated pit pathway in a beta-arrestin- and dynamin-sensitive manner. (duke.edu)
Furthermore, the GIT1 effect is not limited to G protein-coupled receptors because overexpression of this protein also affects internalization of the epidermal growth factor receptor. (duke.edu)
Thus, GIT1 is a protein involved in regulating the function of signaling receptors internalized through the clathrin pathway and can be used as a diagnostic tool for defining the endocytic pathway of a receptor. (duke.edu)
Receptors transduce these signals to alter intracellular metabolism and cellular responsiveness through heterotrimeric G protein/second messenger pathways or through small GTP-binding protein/protein kinase cascades. (duke.edu)
Broadly, my research focuses on the role for G protein-coupled receptors in the pathophysiology of asthma. (duke.edu)
G protein-coupled receptors figure largely in the pathology and treatment of this disease. (duke.edu)
Unlike most GPCRs, including the chemokine receptors, coupling to G i is not required for LPC/G2A-mediated chemotaxis, but coupling to G q/11 and G 12/13 is necessary as judged by inhibition with dominant negative forms of these alpha subunits or with regulators of G-protein signaling (RGS) constructs. (ashpublications.org)
Many types of molecular controls regulate this process, but G-protein-coupled receptors are especially important. (ashpublications.org)
Our laboratory has used the phototransduction pathway in retinal rods, a beautifully designed sensory response system, to study how G protein coupled receptors (GPCRs) propagate highly amplified signals. (cornell.edu)
Research in our laboratory focuses on the elucidation of the molecular and regulatory mechanisms controlling the functionality of a class of proteins called receptors located on the cell surface and to which the brain chemical dopamine attaches and induces their activation. (ohri.ca)
The dopamine receptors (D1R, D2R, D3R, D4R and D5R) are plasma membrane proteins sharing structural and functional similarities with one of the largest family of receptors found in living organisms. (ohri.ca)
Indeed, dopamine receptors belong to the large family of G protein-coupled receptors or GPCRs. (ohri.ca)
The communication or cross-talk between dopamine receptors and its effectors is mediated by the stimulation of molecular switches known as heterotrimeric G proteins on which dopamine receptors initiate a repetitive exchange cycle between two guanosine nucleotides: guanosine diphosphate or GDP-bound G proteins (switch off) guanosine triphosphate or GTP-bound G proteins (switch on). (ohri.ca)
While dopamine receptors mediate their effects on cells through the classical regulation of G proteins, they can also control cell activity in a G protein-independent manner via direct protein-protein interactions with effectors and other intracellular partners. (ohri.ca)
Odorant receptors (ORs) are G protein-coupled receptors (GPCRs) that are essential for detecting and distinguishing among odorants. (bmbreports.org)
Regulators of G protein signaling (RGS) proteins are key players in regulating signaling via G protein-coupled receptors. (unh.edu)
Thus far nearly 20 chemokine receptors have been recognized (Balkwill 2004 Gilman 1987 Pierce Premont & Lefkowitz 2002 Viola & Luster 2008 The large number of chemokines compared to chemokine receptors indicates substantial redundancy in chemokine receptor relationships with multiple ligands binding to the same receptor and vice versa. (scienceexhibitions.org)
Chemokine receptors are pertussis toxin-sensitive GTP-binding proteins of Gi type. (scienceexhibitions.org)
Lysophosphatidic acid (LPA) is a water-soluble phospholipid derivative and a potent signalling molecule that binds to six known lysophosphatidic acid receptors (LPARs), named LPA1-LPA6. (smpdb.ca)
All six receptors belong to the G protein-coupled receptor (GPCR) superfamily which initiates intracellular signalling cascades via four G protein classes differentiated by their α subunit type: Gαs, Gαi/o, Gαq/11, Gα12/13. (smpdb.ca)
It is suggested that G-protein-coupled receptors might modulate cytoskeletal dynamics, intracellular traffic, and cellular architecture. (jneurosci.org)
These peptides typically bind to GPCRs (G-protein-coupled receptors) and activate signaling through heterotrimeric G protein / complexes, which are stimulated by guanine nucleotide exchange of GDP for GTP and inactivated by autocatalytic GTPase activity and reassociation with the bg heterodimer (Fig. 1A). (andreasullivanclarke.com)
Heterotrimeric G proteins are essential mediators of G protein-coupled receptors (GPCRs) signalling to intracellular effectors. (nih.gov)

The GTPase-containing subunits of heterotrimeric GTP-binding proteins. (jefferson.edu)
Cloning of the human cDNA encoding this protein revealed a strong sequence similarity with palmitoyl protein thioesterase (PPT), an enzyme that removes palmitate from H-Ras and the G alpha s subunits of heterotrimeric GTP-binding proteins in vitro. (inrae.fr)

The off-switch is maintained by hydrolysis of GTP to GDP and a phosphate moiety. (biophysics.org)
Hydrolysis of GTP by the inherent GTPase activity of the subunit causes it to revert to its inactive (heterotrimeric) form. (jefferson.edu)
The duration of the signal is determined by the intrinsic GTP hydrolysis rate of the a subunit followed by reassociation of αGDP with βγ. (sigmaaldrich.com)
They include both the hydrolysis resistant GTP analogs, GTP-γ-S and GDP-β-S, that hold the Gα subunit in active and inactive conformations, respectively, and various bacterial toxins. (sigmaaldrich.com)
RGS proteins directly bind to the Gα-subunits of activated heterotrimeric G-proteins, and accelerate the rate of GTP hydrolysis, thereby rapidly deactivating G-proteins. (unh.edu)
Factors that utilize energy from the hydrolysis of GTP to GDP for peptide chain elongation. (childrensmercy.org)

Guanine nucleotide-binding protein G(o) subunit alpha is a protein that in humans is encoded by the GNAO1 gene. (wikipedia.org)
Mutagenesis of the amino terminus of the alpha subunit of the G protein Go. (wikipedia.org)
This study investigates whether the interaction between diuretics and alpha-adducin (ADD1) G460W or G-protein beta3-subunit (GNB3) rs2301339 polymorphism modifies the risk of myocardial infarction (MI) or stroke. (ox.ac.uk)
In the inactive state, GDP is tightly bound to the a subunit of the heterotrimer. (sigmaaldrich.com)
Upon receptor activation GDP is exchanged for GTP, followed by α-subunit dissociation from βγ or alternatively their molecular rearrangement to form active αGTP and βγ complexes. (sigmaaldrich.com)
In addition to the intrinsic GTPase activity of the α subunit, G protein deactivation is accelerated by GTPase activating proteins (GAPs). (sigmaaldrich.com)
This pathway starts with the absorption of a photon by the GPCR rhodopsin, resulting in its activation of the heterotrimeric G protein transducin by catalyzing GDP-GTP exchange on the transducin-alpha subunit (GαT). (cornell.edu)
After a ligand binds to the GPCR, it activates a heterotrimeric G-protein, which is composed of three subunits: a guanine nucleotide binding α-subunit, and a βγ-heterodimer (98). (pancreapedia.org)
Depending on which family the G protein is, it goes on to activate (G αs protein subunit) or inhibit (G αi protein subunit) the membrane-bound cyclase. (pancreapedia.org)
This enzyme also activates the transcription factor NF-KAPPA B and is composed of alpha and beta catalytic subunits, which are protein kinases and gamma, a regulatory subunit. (uchicago.edu)
and Its Interaction with Kaposi Sarcoma-associated Herpes Virus Flice-interacting Protein and IKK Subunit ß by EPR Spectroscopy. (uchicago.edu)
After chemokine binding the heterotrimeric G protein is activated from the exchange of GDP for GTP and dissociates into the GTP-bound α and the βγ subunits (Goldsmith & Dhanasekaran 2007 Mellado Rodriguez-Frade Manes & Martinez 2001 The dissociated βγ subunit activates two major transmission transduction enzymes a phospholipase C-β (PLC-β) which is definitely specific for phosphatidylinositol and a phosphatidylinositol-3-OH kinase (PI3K). (scienceexhibitions.org)
The binding of LPA to an LPAR allosterically activates the heterotrimeric G protein by exchanging GDP for GTP at the G protein's alpha subunit. (smpdb.ca)
Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit. (embl.de)
Members of this family include the signal-transducing G protein beta subunit, as well as other proteins that regulate signal transduction, transcription, pre-mRNA splicing, cytoskeletal organization, and vesicular fusion. (embl.de)

The G protein-coupled receptor (GPCR) kinases (GRKs) phosphorylate and desensitize agonist-occupied GPCRs. (duke.edu)
G2A is a G-protein-coupled receptor (GPCR) involved in immune regulation. (ashpublications.org)
The results of these studies will enable us to further develop a comprehensive mechanistic picture for how an activated G protein regulates its biological effector in phototransduction, and how this signal is rapidly terminated when its stimulation has ceased, as well as provide fundamentally important insights into key steps essential for other GPCR-sensory responses. (cornell.edu)
One mechanism is the binding of an extracellular ligand to a transmembrane G-protein-coupled receptor (GPCR). (pancreapedia.org)
Introduction of the Gα12-coupling BRET assay is expected to accelerate characterization of GPCR actions on this understudied G protein. (nih.gov)

The signal is determined by a small molecule, Guanosine triphosphate (GTP). (biophysics.org)
However, propofol had no effect on the formation of inositol phosphates induced by NaF, an activator of heterotrimeric guanosine triphosphate (GTP)-binding proteins. (silverchair.com)

Recently, we identified a GTPase-activating protein for the ADP ribosylation factor family of small GTP-binding proteins that we call GIT1. (duke.edu)
Much of our current work concerns the RanBP2 complex, which consists of RanBP2 (a large nucleoporin that is also known as Nup358), SUMO-1-conjugated RanGAP1 (the activating protein for the Ran GTPase), and Ubc9 (the conjugating enzyme for the SUMO family of ubiquitin-like modifiers). (nih.gov)

The asymmetrical distribution of Ran-GTP and Ran-GDP drives cargo transport between the nucleus and cytoplasm through karyopherins, a family of nuclear transport carrier proteins that bind to Ran-GTP. (nih.gov)

However, PI3Kβ is also regulated by direct interactions with receptor tyrosine kinases (RTKs) and their tyrosine phosphorylated substrates, and similar to the class II and III PI3Ks, it binds activated Rab5. (elsevierpure.com)
6. Somlyo AP, Somlyo AV. Ca2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase. (almazovcentre.ru)
This protein initially was identified as an interacting partner for the G protein-coupled receptor kinases, and its overexpression was found to affect signaling and internalization of the prototypical beta(2)-adrenergic receptor. (duke.edu)
Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. (duke.edu)
Receptor and G betagamma isoform-specific interactions with G protein-coupled receptor kinases. (duke.edu)
Our major endeavors employ recombinant DNA technologies, RNA interference, biochemical and cell biological approaches to probe the underlying molecular relationships between dopamine receptor structure, G protein and effector regulation, kinases, phosphatases and drug action. (ohri.ca)
Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. (rush.edu)
AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP. (rush.edu)
A subcategory of phosphohydrolases that are specific for MITOGEN-ACTIVATED PROTEIN KINASES. (rush.edu)

Binding of GTP-bound GαT subunits to PDE6 activates its ability to hydrolyze cGMP to GMP, thus closing cGMP-gated ion channels in retinal rod membranes and sending a signal to the optic nerve. (cornell.edu)
Recently, we solved a cryoEM structure for a complex in solution that contains two GTP-bound GαT subunits and PDE6, leading to a model describing how transducin activates its biological effector. (cornell.edu)
The interaction of tubulin with these polypeptides involves a GTP transfer from the exchangeable GTP-binding site (E site) of tubulin to Gα, which activates the G-protein (transactivation) ( Roychowdhury and Rasenick, 1994 ). (jneurosci.org)

We determined structures for the rhodopsin-transducin complex by cryo-electron microscopy (cryoEM), which together with efforts from other laboratories, led to a detailed picture of how GPCRs activate their G protein partners. (cornell.edu)
There is a considerable diversity of G protein subunits that channel signals initiated by GPCRs into specific outcome. (nih.gov)

Proteins which are found in membranes including cellular and intracellular membranes. (curehunter.com)
2010 2 CXCR4/CXCL12 SIGNALING CXCL12 binding to CXCR4 initiates numerous downstream signaling pathways that result in AMG-47a a plethora of reactions (Fig. 2.1) such as increase in intracellular calcium gene transcription chemotaxis cell survival and proliferation (Ganju et al. (scienceexhibitions.org)
Cytoskeletal proteins appear to be involved in the control of intracellular signaling. (jneurosci.org)

A single ligand occupied receptor is able to activate several G protein molecules during the lifetime of a single αGTP complex. (sigmaaldrich.com)
The ultimate pellet was resuspended in remedy A supplemented with 12% (wt/vol) sucrose and aliquots had been frozen and kept at ?80°C. Ligand Binding. (biopaqc.com)
GRPr ligand binding sites in the membrane. (biopaqc.com)
The ligand-binding site is in the extracellular domain and the cytosolic domain has a heterotrimeric G protein-binding site (127). (pancreapedia.org)
1 In general, a resolution of 3Å or better is required to determine the binding mode of a fragment-sized ligand structure, and such resolution can be routinely achieved using cryo-EM. (technologynetworks.com)

Price TJ, Das V, Dussor G. Adenosine Monophosphate-activated Protein Kinase (AMPK) Activators For the Prevention, Treatment and Potential Reversal of Pathological Pain. (rush.edu)
A protein serine-threonine kinase that catalyzes the PHOSPHORYLATION of I KAPPA B PROTEINS. (uchicago.edu)
Both calcium and DAG activate the kinase activity of protein kinase C beta (PKC). (smpdb.ca)

Here, we present and compare the cryo-electron microscopy structures of M1R in complex with G 11 and M2R in complex with G oA The M1R-G 11 complex exhibits distinct features, including an extended transmembrane helix 5 and carboxyl-terminal receptor tail that interacts with G protein. (rcsb.org)
Most mammalian adenylyl cyclases are large transmembrane proteins and subject to complex regulation, most importantly through direct interactions with heterotrimeric GTP-binding (G) proteins (Figure 2 , left-hand side). (biomedcentral.com)
Mammalian adenylyl cyclases (left-hand side) are typically transmembrane proteins activated by heterotrimeric G proteins. (biomedcentral.com)
The receptor protein has seven transmembrane α-helices connected by alternating cytosolic and extracellular loops. (pancreapedia.org)

Conformational dynamics of a regulator of G-protein signaling protein reveals a mechanism of allosteric inhibition by a small molecule. (unh.edu)
Using atomistic simulations and NMR spectroscopy, we have studied in molecular detail the mechanism of action of CCG-50014, a potent small molecule inhibitor of RGS4 that covalently binds to cysteine residues on RGS4. (unh.edu)

In contrast, mammalian guanylyl cyclases are modular receptor enzymes: they exist as homo- or heterodimers with one catalytic domain in each polypeptide chain, and their activity can be modulated directly by hormones or other factors that bind to their accessory domains (Figure 2 , right-hand side) [ 9 ]. (biomedcentral.com)
Mammalian guanylyl cyclases (right-hand side) are receptor enzymes directly activated by hormones, such as nitric oxide (NO), or other factors that bind to its regulatory domains (for simplicity, the regulatory domains are not shown). (biomedcentral.com)
In particular, mammalian genomes contain 16 conserved genes encoding G protein α subunits with unique properties. (nih.gov)

The unusually complex regulation of PI3Kβ by small and trimeric G proteins and RTKs leads to a rich landscape of signaling responses at the cellular and organismic levels. (elsevierpure.com)

The non-functional active site is the binding site for the activator forskolin, a well-known hypotensive agent. (biomedcentral.com)

GTP-Binding Protein alpha Subunits" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
The GTP-Binding protein alpha subunits are grouped into families according to the type of action they have on second messenger systems. (jefferson.edu)
This graph shows the total number of publications written about "GTP-Binding Protein alpha Subunits" by people in this website by year, and whether "GTP-Binding Protein alpha Subunits" was a major or minor topic of these publications. (jefferson.edu)
Below are the most recent publications written about "GTP-Binding Protein alpha Subunits" by people in Profiles. (jefferson.edu)

Here, we report that the guanine nucleotide-binding protein (G protein)-coupled receptor-activated WD-repeat Gβ interacts with the glucocorticoid receptor (GR), comigrates with it into the nucleus and suppresses GR-induced transactivation of the glucocorticoid-responsive genes. (rupress.org)

It is known to act through protein phosphorylation via PRKA and through the activation of guanine nucleotide exchange factors like EPAC. (plos.org)
blue) bound to a nucleotide analog (shown as a stick model). (biomedcentral.com)
Cyclic nucleotides form when the phosphate group of the molecule of nucleotide triphosphate (ATP or GTP) is attacked by the 3' hydroxyl group of the ribose, forming a cyclic 3',5'-phosphate ester with release of pyrophosphate. (pancreapedia.org)

Both enzymes produce phosphatidylinositol 3,4,5-trisposphate in vivo and are regulated by both heterotrimeric G proteins and small GTPases from the Ras or Rho families. (elsevierpure.com)

The specificity of the GIT1 effect is not related to the type of G protein to which a receptor couples, but rather to the endocytic route it uses. (duke.edu)
The specificity of the proteins is determined by the sequences outside the repeats themselves. (embl.de)

However, the only studied example of a WD-repeat protein, G beta, synthesized in vitro in a rabbit reticulocyte lysate, is unable to fold into a native structure without its partner protein G gamma. (embl.de)
It is not known whether all WD-repeat proteins are unable to fold when synthesized in an in vitro system. (embl.de)

GTP-bound GαT subunits then interact with their effector protein, the cyclic GMP (cGMP) phosphodiesterase-6 (PDE6), a tetrameric enzyme with two catalytic subunits (PDEα, PDEβ) and two subunits (PDEγ) that bind GαT. (cornell.edu)
However, there is still a great deal to learn about how activated G proteins execute a precise regulation of their effector proteins. (cornell.edu)
We will now test important aspects of this model through two broad experimental aims, each comprised of a number of sub-aims: 1) Determine how activated Gα subunits of the retinal G protein transducin exert a highly tuned regulation of their biological effector PDE6. (cornell.edu)
2) Establish a mechanistic basis for how a membrane environment influences the ability of the retinal G protein to activate its biological effector. (cornell.edu)

Overexpression of ZmWUS1-B is due to multimerized binding sites for type-B RESPONSE REGULATORs (RRs), key transcription factors in cytokinin signaling. (nature.com)

Endogenous substances, usually proteins, that are involved in the blood coagulation process. (lookformedical.com)

We will use: (i) fluorescence read-outs to monitor GαT-PDE6 interactions to determine how membranes facilitate PDE6 activation by GαT, and (ii) FRET to examine the orientation of the PDEγ subunits on PDE6 in the presence and absence of GTP-bound GαT in a membrane environment. (cornell.edu)

Pasteurella multocida toxin (PMT, produced by Pasteurella multocida ) offers the possibility to discriminate between Gα q and Gα 11 proteins, since it stimulates inositol phosphate formation in a strictly Gα q -dependent manner. (sigmaaldrich.com)
IP3 diffuses through the cytoplasm to the ER and binds to the inositol 1,4,5-trisphosphate (Ins3P) receptor, releasing calcium from the endoplasmic reticulum into the cytoplasm. (smpdb.ca)

This results in the dissociation of a Gα-GTP monomer and a Gβγ dimer from the receptor which allows both complexes to begin signalling cascades via downstream effectors. (smpdb.ca)

1996 Although CXCL12 is the only known chemokine that binds CXCR4 recent studies suggest that extracellular ubiquitin also functions as an immune modulator through CXCR4-mediated signaling (Saini Marchese & Majetschak AMG-47a 2010 Tripathi et al. (scienceexhibitions.org)
2013 Although CXCR4 is known to bind only CXCL12 in 2005 another chemokine receptor CXC receptor 7 (CXCR7 ACKR3 RDC1 CMKOR1 or GPR159) was founded like a receptor for CXCL12 (Balabanian et al. (scienceexhibitions.org)

the Vwf extracted membrane preparations representing a 2- to 3-fold enrichment of binding sites compared with the membranes before extraction. (biopaqc.com)
neuromedin B. Reconstitution of urea extracted membranes with a purified Gαq showed that receptor-catalyzed binding of GTPγS was dependent on agonist (GRP) and Gβγ subunits. (biopaqc.com)

They consist of two types, peripheral and integral proteins. (curehunter.com)
Trafficking between the nucleus and cytoplasm occurs through nuclear pore complexes (NPCs), which consist of ca. thirty distinct proteins called nucleoporins. (nih.gov)

WD-repeat proteins are a large family found in all eukaryotes and are implicated in a variety of functions ranging from signal transduction and transcription regulation to cell cycle control and apoptosis. (embl.de)

they belong to a superfamily of GTPases that includes monomeric proteins such as EF-Tu and RAS. (umassmed.edu)

Mutation of the conserved DRY motif of G2A resulted in loss of chemotaxis to LPC, suggesting a requirement for G-protein signaling. (ashpublications.org)

Heterotrimeric G-proteins are molecular switches that are omnipresent in animal and plant cells. (biophysics.org)

The signal imparted by the binding of a single agonist to its receptor is thus transduced and amplified leading to generation of several active αGTP and βγ molecules during the lifetime of the first αGTP. (sigmaaldrich.com)
Because most tubulin is not membrane associated, this study investigates whether tubulin translocates to the membrane in response to an agonist so that it might regulate G-protein signaling. (jneurosci.org)

GAPs for heterotrimeric G proteins include G protein effectors, such as the Gα q -dependent phospholipase Cβ and the Gα 13 -dependent p115RhoGEF, as well as the family of regulators of G protein signaling (RGS proteins). (sigmaaldrich.com)

Exploring the role of insulin-like growth factor binding protein-1 in identifying idiopathic multicentric Castleman's disease types: Implications for the mTOR signaling pathway. (harvard.edu)

The microtubule protein tubulin regulates adenylyl cyclase and phospholipase Cβ 1 (PLCβ 1 ) signaling via transactivation of the G-protein subunits Gαs, Gαi1, and Gαq. (jneurosci.org)

This cyclic conformation allows cyclic nucleotides to bind to proteins to which other nucleotides cannot. (pancreapedia.org)

In the cytosol, the signal molecule binds to the heme-binding domain of the soluble cyclase. (pancreapedia.org)

Although a high level of PDE10A gene expression is observed in the testis, information on the identity of the isoforms or on the cell type that express the PDE10 protein is lacking. (plos.org)

Structural basis for the selectivity of the RGS protein, GAIP, for Galphai family members. (wikipedia.org)
Detailed analysis of these structures provides a framework for understanding the molecular determinants of G-protein coupling selectivity. (rcsb.org)
These methods AM 694 have allowed the examination of G protein selectivity using purified G protein subunits as well as extended our knowledge of the molecular pharmacology of the GRPr. (biopaqc.com)

Anatomy22

Organisms9

Chemicals and Drugs156

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes46

Information Science3

Receptors32

Subunits of heterotrimeric GTP-bind2

Hydrolysis6

Subunit15

GPCR5

Guanosine triphosphate2

GTPase2

Carrier Proteins1

Kinases9

Activates3

GPCRs2

Intracellular3

Ligand5

Kinase3

Transmembrane4

Small molecule2

Mammalian3

Cellular1

Activator1

Alpha subunits4

Guanine nucleot1

Nucleotide3

Enzymes1

Specificity2

Vitro2

Effector4

Transcription factors1

Substances1

Interactions1

Inositol2

Dissociation1

Chemokine2

Membranes2

Consist2

Regulation1

Superfamily1

Mutation1

Molecular switches1

Agonist2

Effectors1

Disease1

Phospholipase1

Cyclic1

Signal1

Isoforms1

Selectivity3